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hyaluronan matrix

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https://www.readbyqxmd.com/read/29346283/the-biological-role-of-hyaluronan-rich-oocyte-cumulus-extracellular-matrix-in-female-reproduction
#1
REVIEW
Eva Nagyova
Fertilization of the mammalian oocyte requires interactions between spermatozoa and expanded cumulus extracellular matrix (ECM) that surrounds the oocyte. This review focuses on key molecules that play an important role in the formation of the cumulus ECM, generated by the oocyte-cumulus complex. In particular, the specific inhibitors (AG1478, lapatinib, indomethacin and MG132) and progesterone receptor antagonist (RU486) exerting their effects through the remodeling of the ECM of the cumulus cells surrounding the oocyte have been described...
January 18, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29339093/the-proteoglycan-biglycan-mediates-inflammatory-response-by-activating-tlr-4-in-human-chondrocytes-inhibition-by-specific-sirna-and-high-polymerized-hyaluronan
#2
Angela Avenoso, Angela D'Ascola, Michele Scuruchi, Giuseppe Mandraffino, Alberto Calatroni, Antonino Saitta, Salvatore Campo, Giuseppe M Campo
Cartilage degeneration are hallmarks of wear, tear, mechanical and inflammatory damage of the joint cartilage. Tissue degradation as well as compromising the integrity and function of the organ, produces different intermediates, directly able to stimulate further inflammatory effect, therefore, amplifying the inflammation response. Biglycan is a soluble component of the extracellular matrix that is released during tissue injury. It has been reported that released biglycan is an endogenous ligand for TLR-2/4 in some cell type...
January 12, 2018: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29331336/udp-sugar-accumulation-drives-hyaluronan-synthesis-in-breast-cancer
#3
Sanna Oikari, Tiia Kettunen, Satu Tiainen, Jukka Häyrinen, Amro Masarwah, Mazen Sudah, Anna Sutela, Ritva Vanninen, Markku Tammi, Päivi Auvinen
Increased uptake of glucose, a general hallmark of malignant tumors, leads to an accumulation of intermediate metabolites of glycolysis. We investigated whether the high supply of these intermediates promotes their flow into UDP-sugars, and consequently into hyaluronan, a tumor-promoting matrix molecule. We quantified UDP-N-Acetylglucosamine (UDP-GlcNAc) and UDP-glucuronic acid (UDP-GlcUA) in human breast cancer biopsies, the levels of enzymes contributing to their synthesis, and their association with the hyaluronan accumulation in the tumor...
January 10, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29322079/fibroblast-and-keratinocyte-gene-expression-following-exposure-to-extracts-of-neem-plant-azadirachta-indica
#4
Takao Someya, Katsura Sano, Kotaro Hara, Yoshimasa Sagane, Toshihiro Watanabe, R G S Wijesekara
This data article provides gene expression profiles, determined by using real-time PCR, of fibroblasts and keratinocytes treated with 0.01% and 0.001% extracts of neem plant (Azadirachta indica), local name "Kohomba" in Sri Lanka, harvested in Sri Lanka. For fibroblasts, the dataset includes expression profiles for genes encoding hyaluronan synthase 1 (HAS1), hyaluronan synthase 2 (HAS2), hyaluronidase-1 (HYAL1), hyaluronidase-2 (HYAL2), versican, aggrecan, CD44, collagen, type I, alpha 1 (COL1A1), collagen, type III, alpha 1 (COL3A1), collagen, type VII, alpha 1 (COL7A1), matrix metalloproteinase 1 (MMP1), acid ceramidase, basic fibroblast growth factor (bFGF), fibroblast growth factor-7 (FGF7), vascular endothelial growth factor (VEGF), interleukin-1 alpha (IL-1α), cyclooxygenase-2 (cox2), transforming growth factor beta (TGF-β), and aquaporin 3 (AQP3)...
February 2018: Data in Brief
https://www.readbyqxmd.com/read/29315518/hyaluronan-binding-by-cd44-reduces-the-memory-potential-of-activated-murine-cd8-t-cells
#5
Sally S M Lee-Sayer, Nina Maeshima, Meghan N Dougan, Anita Dahiya, Arif A Arif, Manisha Dosanjh, Christopher A Maxwell, Pauline Johnson
Expansion and death of effector CD8 T cells are regulated to limit immunopathology and cells that escape contraction go on to generate immunological memory. CD44, a receptor for the extracellular matrix component hyaluronan, is a marker of activated and memory T cells. Here, we show with a murine model that the increase in CD44 expression and hyaluronan binding induced upon CD8 T cell activation was proportional to the strength of TCR engagement, thereby identifying the most strongly activated T cells. When CD44-/- and CD44+/+ OT-I CD8 T cells were adoptively transferred into mice challenged with Listeria-OVA, there was a slight increase in the percentage of CD44+/+ cells at the effector site...
January 8, 2018: European Journal of Immunology
https://www.readbyqxmd.com/read/29310782/the-biochemistry-and-immunohistochemistry-of-versican
#6
Stephen P Evanko, Christina K Chan, Pamela Y Johnson, Charles W Frevert, Thomas N Wight
Versican is a chondroitin sulfate proteoglycan found in the extracellular matrix that is important for changes in cell phenotype associated with development and disease. Versican has been shown to be involved in cardiovascular disorders, as well as lung disease and fibrosis, inflammatory bowel disease, cancer, and several other diseases that have an inflammatory component. Versican was first identified as a fibroblast proteoglycan and forms large multimolecular complexes with hyaluronan and other components of the provisional matrix during wound healing and inflammation...
2018: Methods in Cell Biology
https://www.readbyqxmd.com/read/29289686/the-therapeutic-potential-of-hyaluronan-in-copd
#7
Gerard M Turino, Shuren Ma, Yong Y Lin, Jerome O Cantor
Insights into the clinical course in COPD indicate the need for new therapies in COPD. The discovery of alpha-1 antitrypsin deficiency (AATD) led to the protease-antiprotease imbalance hypothesis which was applied to COPD related and not related to AATD. The discovery of AATD brought recognition to the importance of elastin fibers in maintaining lung matrix structure. Two cross-linking amino acids are unique to mature elastin, desmosine and isodesmosine (DI), which can serve as biomarkers of the degradation of elastin...
December 28, 2017: Chest
https://www.readbyqxmd.com/read/29287161/evaluation-of-equine-synovial-derived-extracellular-matrix-scaffolds-seeded-with-equine-synovial-derived-mesenchymal-stem-cells
#8
Nathalie A Reisbig, Hayam A Hussein, Erin Pinnell, Alicia L Bertone
OBJECTIVE To create a bioactive synovium scaffold by infusing decellularized synovial-derived extracellular matrix (synECM) with synovial-derived mesenchymal stem cells (synMSCs). SAMPLE Synovium from the femoropatellar and medial femorotibial joints of equine cadavers. PROCEDURES The synMSCs were cultured in monolayer and not treated or cotransduced to enhance expression of green fluorescent protein (GFP) and human bone morphogenetic protein (BMP)-2. The synECM was decellularized with 0.1% peracetic acid and then seeded with synMSCs (0...
January 2018: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/29282520/tgf%C3%AE-counteracts-lyve-1-mediated-induction-of-lymphangiogenesis-by-small-hyaluronan-oligosaccharides
#9
Jochen Bauer, Melanie Rothley, Anja Schmaus, Luca Quagliata, Markus Ehret, Moritz Biskup, Véronique Orian-Rousseau, David G Jackson, Ronald J Pettis, Alfred Harvey, Stefan Bräse, Wilko Thiele, Jonathan P Sleeman
During tissue injury, inflammation, and tumor growth, enhanced production and degradation of the extracellular matrix glycosaminoglycan hyaluronan (HA) can lead to the accumulation of small HA (sHA) oligosaccharides. We have previously reported that accumulation of sHA in colorectal tumors correlates with lymphatic invasion and lymph node metastasis, and therefore, investigated here are the effects of sHA on the lymphatic endothelium. Using cultured primary lymphatic endothelial cells (LECs) and ex vivo and in vivo lymphangiogenesis assays, we found that in contrast to high-molecular-weight HA (HMW-HA), sHA of 4-25 disaccharides in length can promote the proliferation of LECs and lymphangiogenesis in a manner that is dependent on their size and concentration...
December 27, 2017: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/29247178/changes-in-hyaluronan-deposition-in-the-rat-myenteric-plexus-after-experimentally-induced-colitis
#10
Viviana Filpa, Michela Bistoletti, Ilaria Caon, Elisabetta Moro, Annalisa Grimaldi, Paola Moretto, Andreina Baj, Maria Cecilia Giron, Evgenia Karousou, Manuela Viola, Francesca Crema, Gianmario Frigo, Alberto Passi, Cristina Giaroni, Davide Vigetti
Myenteric plexus alterations hamper gastrointestinal motor function during intestinal inflammation. Hyaluronan (HA), an extracellular matrix glycosaminoglycan involved in inflammatory responses, may play a role in this process. In the colon of control rats, HA-binding protein (HABP), was detected in myenteric neuron soma, perineuronal space and ganglia surfaces. Prominent hyaluronan synthase 2 (HAS2) staining was found in myenteric neuron cytoplasm, suggesting that myenteric neurons produce HA. In the myenteric plexus of rats with 2, 4-dinitrobenzene sulfonic (DNBS)-induced colitis HABP staining was altered in the perineuronal space, while both HABP staining and HA levels increased in the muscularis propria...
December 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29232172/halo-202-randomized-phase-ii-study-of-pegph20-plus-nab-paclitaxel-gemcitabine-versus-nab-paclitaxel-gemcitabine-in-patients-with-untreated-metastatic-pancreatic-ductal-adenocarcinoma
#11
Sunil R Hingorani, Lei Zheng, Andrea J Bullock, Tara E Seery, William P Harris, Darren S Sigal, Fadi Braiteh, Paul S Ritch, Mark M Zalupski, Nathan Bahary, Paul E Oberstein, Andrea Wang-Gillam, Wilson Wu, Dimitrios Chondros, Ping Jiang, Sihem Khelifa, Jie Pu, Carrie Aldrich, Andrew E Hendifar
Purpose Metastatic pancreatic ductal adenocarcinoma is characterized by excessive hyaluronan (HA) accumulation in the tumor microenvironment, elevating interstitial pressure and impairing perfusion. Preclinical studies demonstrated pegvorhyaluronidase alfa (PEGPH20) degrades HA, thereby increasing drug delivery. Patients and Methods Patients with previously untreated metastatic pancreatic ductal adenocarcinoma were randomly assigned to treatment with PEGPH20 plus nab-paclitaxel/gemcitabine (PAG) or nab-paclitaxel/gemcitabine (AG)...
December 12, 2017: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/29229920/integration-of-neurogenesis-and-angiogenesis-models-for-constructing-a-neurovascular-tissue
#12
Hiroyuki Uwamori, Takuya Higuchi, Ken Arai, Ryo Sudo
Neurovascular unit (NVU) is a basic unit in the brain, including neurons, glial cells, blood vessels and extracellular matrix. This concept implies the importance of a three-dimensional (3D) culture model including these cell types for investigating brain functions. However, little is known about the construction of an in vitro 3D NVU model. In the present study, we aimed at constructing 3D neurovascular tissues by combining in vitro neurogenesis and angiogenesis models using a microfluidic platform, which is a critical step toward the NVU construction in vitro...
December 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29211034/glucocorticoids-improve-myogenic-differentiation-in-vitro-by-suppressing-the-synthesis-of-versican-a-transitional-matrix-protein-overexpressed-in-dystrophic-skeletal-muscles
#13
Natasha McRae, Leonard Forgan, Bryony McNeill, Alex Addinsall, Daniel McCulloch, Chris Van der Poel, Nicole Stupka
In Duchenne muscular dystrophy (DMD), a dysregulated extracellular matrix (ECM) directly exacerbates pathology. Glucocorticoids are beneficial therapeutics in DMD, and have pleiotropic effects on the composition and processing of ECM proteins in other biological contexts. The synthesis and remodelling of a transitional versican-rich matrix is necessary for myogenesis; whether glucocorticoids modulate this transitional matrix is not known. Here, versican expression and processing were examined in hindlimb and diaphragm muscles from mdx dystrophin-deficient mice and C57BL/10 wild type mice...
December 6, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29210510/glycosaminoglycan-and-proteoglycan-based-biomaterials-current-trends-and-future-perspectives
#14
REVIEW
Jelena Rnjak-Kovacina, Fengying Tang, John M Whitelock, Megan S Lord
Proteoglycans and their glycosaminoglycans (GAG) are essential for life as they are responsible for orchestrating many essential functions in development and tissue homeostasis, including biophysical properties and roles in cell signaling and extracellular matrix assembly. In an attempt to capture these biological functions, a range of biomaterials are designed to incorporate off-the-shelf GAGs, typically isolated from animal sources, for tissue engineering, drug delivery, and regenerative medicine applications...
December 6, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29204833/the-macula-flava-of-the-human-vocal-fold-as-a-stem-cell-microenvironment
#15
Kiminori Sato
1. There is growing evidence to suggest that the cells in the maculae flavae are tissue stem cells of the human vocal fold and maculae flavae are a candidate for a stem cell niche. 2. The latest research shows that the cells in the human maculae flavae are involved in the metabolism of extracellular matrices that are essential for the viscoelasticity in the human vocal fold mucosa as a vibrating tissue, and considered to be important cells in the growth, development, and aging of the human vocal fold mucosa...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29186441/imaging-specific-cellular-glycan-structures-using-glycosyltransferases-via-click-chemistry
#16
Zhengliang L Wu, Anthony Person, Matthew Anderson, Barbara Burroughs, Timothy Tatge, Kshitij Khatri, Yonglong Zou, Lianchun Wang, Todd Geders, Joseph Zaia, Robert Sackstein
Heparan sulfate (HS) is a polysaccharide fundamentally important for biologically activities. T/Tn antigens are universal carbohydrate cancer markers. Here, we report the specific imaging of these carbohydrates using a mesenchymal stem cell line and human umbilical vein endothelial cells (HUVEC). The staining specificities were demonstrated by comparing imaging of different glycans and validated by either removal of target glycans, which results in loss of signal, or installation of target glycans, which results in gain of signal...
November 25, 2017: Glycobiology
https://www.readbyqxmd.com/read/29184125/stiffness-dependent-motility-and%C3%A2-proliferation-uncoupled-by-deletion-of-cd44
#17
Ziba Razinia, Paola Castagnino, Tina Xu, Alexandra Vázquez-Salgado, Ellen Puré, Richard K Assoian
Information in the microenvironment guides complex cellular decisions such as whether or not to proliferate and migrate. The effects of soluble extracellular signals on these cellular functions are fairly well understood, but relatively little is known about how the extracellular matrix (ECM), and particularly the mechanical information in the ECM, guides these cellular decisions. Here, we show that CD44, a major receptor for the glycosaminoglycan ECM component hyaluronan, coordinates the motility and proliferative responses to ECM stiffening...
November 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29183997/hyaluronan-content-governs-tissue-stiffness-in-pancreatic-islet-inflammation
#18
Nadine Nagy, Adi de la Zerda, Gernot Kaber, Pamela Y Johnson, Kenneth H Hu, Michael J Kratochvil, Koshika Yadava, Wenting Zhao, Yi Cui, Guadalupe Navarro, Justin P Annes, Thomas N Wight, Sarah C Heilshorn, Paul L Bollyky, Manish J Butte
We have identified a novel role for hyaluronan (HA), an extracellular matrix (ECM) polymer, in governing the mechanical properties of inflamed tissues. We recently reported that insulitis in type 1 diabetes (T1D) of mice and humans is preceded by intra-islet accumulation of HA, a highly hygroscopic polymer. Using the DORmO double transgenic (DO11.10 x RIPmOVA) mouse model of T1D, we asked whether autoimmune insulitis was associated with changes in the stiffness of islets. To measure islet stiffness, we used atomic force microscopy (AFM) and developed a novel "bed of nails"-like approach that uses quartz glass nanopillars to anchor islets, solving a long-standing problem of keeping tissue-scale objects immobilized while performing AFM...
November 28, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29178472/enzymatic-degradation-of-hyaluronan-hydrogels-with-different-capacity-for-in-situ-bio-mineralization
#19
Liyang Shi, Yu Zhang, Dmitri Ossipov
In situ cross-linked hyaluronan (HA) hydrogels with different capacities for biomineralization were prepared and their enzymatic degradation was monitored. Covalent incorporation of bisphosphonates (BPs) into HA hydrogel results in the increased stiffness of the hydrogel in comparison with the unmodified HA hydrogel of the same cross-linking density. The rate of enzymatic degradation of HABP hydrogel was significantly lower than the rate of degradation of control HA hydrogel in vitro. This effect is observed only in the presence of calcium ions that strongly bind to the matrix-anchored BP groups and promote further mineralization of the matrix...
November 27, 2017: Biopolymers
https://www.readbyqxmd.com/read/29175954/cd44-signaling-mediates-high-molecular-weight-hyaluronan-induced-anti-hyperalgesia
#20
Luiz F Ferrari, Eugen V Khomula, Dioneia Araldi, Jon D Levine
We studied, in male Sprague-Dawley rats, the role of the cognate hyaluronan (HA) receptor, CD44 signaling in the anti-hyperalgesia induced by high molecular weight hyaluronan (HMWH). Low molecular weight hyaluronan (LMWH) acts at both peptidergic and non-peptidergic nociceptors to induce mechanical hyperalgesia that is prevented by intrathecal oligodeoxynucleotide (ODN) antisense to CD44 mRNA, which also prevents hyperalgesia induced by a CD44 receptor agonist, A6. Ongoing LMWH and A6 hyperalgesia is reversed by HMWH...
November 23, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
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