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hyaluronan matrix

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https://www.readbyqxmd.com/read/28196904/extracellular-matrix-receptor-expression-in-subtypes-of-lung-adenocarcinoma-potentiates-outgrowth-of-micrometastases
#1
Laura E Stevens, William Kc Cheung, Sally J Adua, Anna Arnal-Estapé, Minghui Zhao, Zongzhi Liu, Kelly Brewer, Roy S Herbst, Don X Nguyen
Mechanisms underlying the propensity of latent lung adenocarcinoma (LUAD) to relapse are poorly understood. In this study, we show how differential expression of a network of extracellular matrix (ECM) molecules and their interacting proteins contributes to risk of relapse in distinct LUAD subtypes. Overexpression of the hyaluronan receptor HMMR in primary LUAD was associated with an inflammatory molecular signature and poor prognosis. Attenuating HMMR in LUAD cells diminished their ability to initiate lung tumors and distant metastases...
February 14, 2017: Cancer Research
https://www.readbyqxmd.com/read/28188289/human-keratinocytes-respond-to-extracellular-utp-by-induction-of-hyaluronan-synthase-2-expression-and-increased-hyaluronan-synthesis
#2
Tiina A Jokela, Riikka Kärnä, Leena Rauhala, Genevieve Bart, Sanna Pasonen-Seppänen, Sanna Oikari, Markku I Tammi, Raija H Tammi
The release of nucleotides into extracellular space is triggered by insults like wounding and ultraviolet radiation, resulting in stimulatory or inhibitory signals via plasma membrane nucleotide receptors. As similar insults are known to activate hyaluronan synthesis we explored the possibility that extracellular UTP or its breakdown products UDP and UMP act as mediators for hyaluronan synthase (HAS) activation in human epidermal keratinocytes. UTP increased hyaluronan both in the pericellular matrix and in the culture medium of HaCaT cells...
February 10, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28183614/evaluation-of-cell-surface-interaction-using-a-3d-spheroid-cell-culture-model-on-artificial-extracellular-matrices
#3
Wolfgang Metzger, Sandra Rother, Tim Pohlemann, Stephanie Möller, Matthias Schnabelrauch, Vera Hintze, Dieter Scharnweber
Since decades, cell-surface interactions are studied in 2D cell culture approaches, but cells organized in 3D (spheroids) reflect the normal situation of cells in tissues much better due to intense cell-cell-contacts. Accordingly, this study aimed to prove, if spheroids could be used to study cell-surface interaction. Spheroids consisting of fibroblasts and/or osteoblasts were seeded on artificial extracellular matrices. Here, non-sulfated hyaluronan as a biological relevant compound of the extracellular matrix was chemically sulfated to different extents and co-fibrillised with collagen...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28168629/role-of-hyal1-expression-in-primary-breast-cancer-in-the-formation-of-brain-metastases
#4
Isabell Witzel, Anna K Marx, Volkmar Müller, Harriet Wikman, Jakob Matschke, Udo Schumacher, Christine Stürken, Peter Prehm, Elena Laakmann, Barbara Schmalfeldt, Karin Milde-Langosch, Leticia Oliveira-Ferrer
BACKGROUND: The incidence of brain metastases in breast cancer patients has increased in the last years. However, the knowledge about tumor cell invasion in the brain is still very limited. Based on our recent study on cDNA microarray data of breast cancer patients, we hypothesized that two enzymes involved in the hyaluronan metabolism, namely, hyaluronan synthase 2 (HAS2) and hyaluronidase 1 (HYAL1) are associated with brain metastases formation. METHODS: Protein expression levels of hyaluronan, HAS2, and HYAL1 were analyzed in primary breast cancer, and metastatic tissue samples from different localizations (brain, bone, skin, liver, and lung) were included in four different cohorts by immunohistochemistry...
February 6, 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/28166932/hyaluronan-mediates-the-adhesion-of-porcine-peripheral-blood-mononuclear-cells-to-poly-i-c-treated-intestinal-cells-and-modulates-their-cytokine-production
#5
María José Docampo, Jennifer Cabrera, Anna Bassols
Hyaluronan (HA), a major component of the extracellular matrix (ECM), has been increasingly recognized as a regulator of inflammation. Its role is complex since it has pro- and anti-inflammatory actions by modulating the expression of inflammatory genes, the recruitment of inflammatory cells and the production of inflammatory cytokines, but also by attenuating the course of inflammation and providing protection against tissue damage. Certain viruses and other inflammatory stimuli induce organization of HA into cable-like structures, which may be responsible for leukocyte recruitment and, on the other hand, low molecular weight fragments of HA have been shown to activate various inflammatory responses...
February 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28140522/hybrid-extracellular-matrix-design-for-cartilage-mediated-bone-regeneration
#6
Paiyz E Mikael, Hyun S Kim, Syam P Nukavarapu
Recapitulating long bone repair through endochondral ossification (EO) is increasingly becoming a more popular approach. A successful EO Process depends greatly on the establishment of a healthy hypertrophic-cartilage template (HCT). The aim of this work is to design a hydrogel system, which closely mimics the extracellular matrix of HCT. We examined the combinatorial effect of two commonly used hydrogels for bone and cartilage regeneration strategies, hyaluronan (HA) and fibrin (FB), to induce HCT formation...
January 31, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28125209/dual-functional-hydrazide-reactive-and-anhydride-containing-oligomeric-hydrogel-building-blocks
#7
Christian Kascholke, Tina Loth, Caroline Kohn-Polster, Stephanie Möller, Peter Bellstedt, Michaela Schulz-Siegmund, Matthias Schnabelrauch, Michael C Hacker
Biomimetic hydrogels are advanced biomaterials that have been developed following different synthetic routes. Covalent postfabrication functionalization is a promising strategy to achieve efficient matrix modification decoupled of general material properties. To this end, dual-functional macromers were synthesized by free radical polymerization of maleic anhydride with diacetone acrylamide (N-(1,1-dimethyl-3-oxobutyl)acrylamide) and pentaerythritol diacrylate monostearate. Amphiphilic oligomers (Mn < 7.5 kDa) with anhydride contents of 7-20% offered cross-linking reactivity to yield rigid hydrogels with gelatinous peptides (E = 4-13 kPa) and good cell adhesion properties...
February 8, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28123575/anti-tumor-and-anti-invasion-effects-of-a-combination-of-4-methylumbelliferone-and-ionizing-radiation-in-human-fibrosarcoma-cells
#8
Ryo Saga, Satoru Monzen, Mitsuru Chiba, Hironori Yoshino, Toshiya Nakamura, Yoichiro Hosokawa
Hyaluronan (HA) is a major component of the extracellular matrix that is synthesized in excess in cancer tissues. 4-methylumbelliferone (MU) inhibits the synthesis of HA and is closely related to the invasion and metastasis of cancer. However, the effects of MU in conjunction with cancer radiotherapy remain unknown. The present study assessed the anti-tumor and anti-invasion effects of the concomitant use of ionizing radiation (IR) and 100 µM MU on human fibrosarcoma HT1080 cells. Cell viability and cellular invasion potency assays were performed...
January 2017: Oncology Letters
https://www.readbyqxmd.com/read/28110937/adult-human-retinal-m%C3%A3-ller-glia-display-distinct-peripheral-and-macular-expression-of-cd117-and-cd44-stem-cell-associated-proteins
#9
Lay Khoon Too, Gary Gracie, Enisa Hasic, Julia H Iwakura, Svetlana Cherepanoff
Experimental evidence suggests human Müller glia exhibit neural progenitor properties in vitro. CD117 and CD44 are known to be expressed by stem cells, the survival of which appears to depend critically on interactions with hyaluronan-rich extracellular matrix (ECM). Here, we characterise Müller glia expression of CD117 and CD44 in normal adult human retina and describe how it correlates with hyaluronan distribution in ocular ECM. By using chromogen-based immunohistochemistry, CD117 expression was found in entire Müller glia cytoplasm spanning from inner to outer limiting membrane in both peripheral retina (PR) and macular retina (MR), mirroring expression of the established Müller glia marker vimentin...
January 19, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28109952/ha-metabolism-in-skin-homeostasis-and-inflammatory-disease
#10
REVIEW
Rafaela-Maria Kavasi, Aikaterini Berdiaki, Ioanna Spyridaki, Emanuela Corsini, Aristidis Tsatsakis, George Tzanakakis, Dragana Nikitovic
Hyaluronan (HA), an unsulfated glycosaminoglycan, is an important component of the complex extracellular matrix network which surrounds and supports cells in tissues. HA is detected in all vertebrate tissues, but the bulk of HA is produced and deposited in the skin. In this review we focus on the role of HA in skin-associated inflammatory disease and wound healing. Properties of HA are directly dependent on its molecular weight. Thus, high molecular weight HA (HMWHA) is deposited in normal tissues during homeostasis and promotes their stability whereas low molecular weight HA fragments (LMWHA), on the other hand, may arise from enzymatic or chemical activities...
January 18, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28107185/hyaluronan-synthase-3-mediated-oncogenic-action-through-forming-inter-regulation-loop-with-tumor-necrosis-factor-alpha-in-oral-cancer
#11
Yi-Zih Kuo, Wei-Yu Fang, Cheng-Chih Huang, Sen-Tien Tsai, Yi-Ching Wang, Chih-Li Yang, Li-Wha Wu
Hyaluronan (HA) is a major extracellular matrix component. However, its role and mediation in oral cancer remains elusive. Hyaluronan synthase 3 (HAS3), involved in pro-inflammatory short chain HA synthesis, was the predominant synthase in oral cancer cells and tissues. HAS3 overexpression significantly increased oral cancer cell migration, invasion and xenograft tumorigenesis accompanied with the increased expression of tumor necrosis factor alpha (TNF-α) and monocyte chemoattractant protein 1 (MCP-1). Conversely, HAS3 depletion abrogated HAS3-mediated stimulation...
January 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/28081210/mutations-in-hyal2-encoding-hyaluronidase-2-cause-a-syndrome-of-orofacial-clefting-and-cor-triatriatum-sinister-in-humans-and-mice
#12
Martina M A Muggenthaler, Biswajit Chowdhury, S Naimul Hasan, Harold E Cross, Brian Mark, Gaurav V Harlalka, Michael A Patton, Miho Ishida, Elijah R Behr, Sanjay Sharma, Kenneth Zahka, Eissa Faqeih, Brian Blakley, Mike Jackson, Melissa Lees, Vernon Dolinsky, Leroy Cross, Philip Stanier, Claire Salter, Emma L Baple, Fowzan S Alkuraya, Andrew H Crosby, Barbara Triggs-Raine, Barry A Chioza
Orofacial clefting is amongst the most common of birth defects, with both genetic and environmental components. Although numerous studies have been undertaken to investigate the complexities of the genetic etiology of this heterogeneous condition, this factor remains incompletely understood. Here, we describe mutations in the HYAL2 gene as a cause of syndromic orofacial clefting. HYAL2, encoding hyaluronidase 2, degrades extracellular hyaluronan, a critical component of the developing heart and palatal shelf matrix...
January 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28077237/interplay-of-extracellular-matrix-and-leukocytes-in-lung-inflammation
#13
REVIEW
Thomas N Wight, Charles W Frevert, Jason S Debley, Stephen R Reeves, William C Parks, Steven F Ziegler
During inflammation, leukocytes influx into lung compartments and interact with extracellular matrix (ECM). Two ECM components, versican and hyaluronan, increase in a range of lung diseases. The interaction of leukocytes with these ECM components controls leukocyte retention and accumulation, proliferation, migration, differentiation, and activation as part of the inflammatory phase of lung disease. In addition, bronchial epithelial cells from asthmatic children co-cultured with human lung fibroblasts generate an ECM that is adherent for monocytes/macrophages...
February 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28069922/mechanisms-of-nmda-receptor-and-voltage-gated-l-type-calcium-channel-dependent-hippocampal-ltp-critically-rely-on-proteolysis-that-is-mediated-by-distinct-metalloproteinases
#14
Grzegorz Wiera, Daria Nowak, Inge Van Hove, Piotr Dziegiel, Lieve Moons, Jerzy W Mozrzymas
: Long-term potentiation (LTP) is widely perceived as a memory substrate and in the hippocampal CA3-CA1 pathway, distinct forms of LTP depend on N-methyl-D-aspartate (NMDA) receptors (nmdaLTP) or L-type voltage-gated calcium channels (vdccLTP). Long-term potentiation is also known to be effectively regulated by extracellular proteolysis that is mediated by various enzymes. Herein, we investigated whether in mice hippocampal slices these distinct forms of LTP are specifically regulated by different metalloproteinases (MMPs)...
January 9, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28053085/inhibition-of-mammalian-glycoprotein-ykl-40-identification-of-the-physiological-ligand
#15
Abhishek A Kognole, Christina M Payne
YKL-40 is a mammalian glycoprotein associated with progression, severity, and prognosis of chronic inflammatory diseases and a multitude of cancers. Despite this well-documented association, identification of the lectin's physiological ligand, and accordingly, biological function, has proven experimentally difficult. YKL-40 has been shown to bind chito-oligosaccharides; however, the production of chitin by the human body has not yet been documented. Possible alternative ligands include proteoglycans, polysaccharides, and fibers like collagen, all of which makeup the extracellular matrix...
January 4, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28038750/a-tracheal-scaffold-of-gelatin-chondroitin-sulfate-hyaluronan-polyvinyl-alcohol-with-orientated-porous-structure
#16
Xueping Yu, Guowen Qian, Si Chen, Dong Xu, Xiujuan Zhao, Chang Du
Scaffold of gelatin-chondroitin sulfate-hyaluronan-polyvinyl alcohol (GCH-PVA) with orientated microtubule structure and good hydrophilicity was fabricated by unidirectional freeze-drying method mimicking the composition and structure of tracheal cartilage extracellular matrix. PVA was incorporated to improve flexibility and viscoelasticity of GCH scaffold. All wet scaffolds showed similar compressive elastic modulus with native cartilage. GCH-PVA scaffolds showed high relative remaining stress during relaxation indicating good mechanical stability...
March 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28003146/injectable-hyaluronic-acid-down-regulates-interferon-signaling-molecules-igfbp3-and-ifit3-in-the-bovine-intervertebral-disc
#17
Zepur Kazezian, Zhen Li, Mauro Alini, Sibylle Grad, Abhay Pandit
: Low back pain which is a major cause of disability for people aged between 20 and 50 years imposes a serious socio-economic burden. The current focus of regenerative medicine is on identifying molecular markers to facilitate the design of targeted therapeutics. Previously, we have demonstrated that expression of the anti-proliferative interferon-induced protein with tetratricopeptide repeats 3 (IFIT3) and pro-apoptotic insulin-like growth factor-binding protein-3 (IGFBP3), are up-regulated as downstream targets of the inflammatory cytokine interferon α (IFNα) signaling pathway in the human annulus fibrosus (AF)...
December 18, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27987780/collagen-glycosaminoglycan-coatings-enhance-new-bone-formation-in-a-critical-size-bone-defect-a-pilot-study-in-rats
#18
Yvonne Förster, Ricardo Bernhardt, Vera Hintze, Stephanie Möller, Matthias Schnabelrauch, Dieter Scharnweber, Stefan Rammelt
Bone regeneration in critical size bone defects still represents an important but unsolved clinical problem. Glycosaminoglycans (GAGs) like chondroitin sulfate (CS) or hyaluronan (HA) are important multifunctional components of the extracellular matrix (ECM) in bone and may stimulate bone healing by recruitment of mesenchymal stromal cells and by supporting their differentiation. Sulfation of GAGs affects their biological activity and thus their interactions with growth factors and/or cells involved in the bone healing process...
February 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27982477/crosstalk-between-t-lymphocytes-and-lung-fibroblasts-generation-of-a-hyaluronan-enriched-extracellular-matrix-adhesive-for-monocytes
#19
Léa Gaucherand, Ben A Falk, Stephen P Evanko, Gail Workman, Christina K Chan, Thomas N Wight
In immunity and inflammation, T cells are often associated with stromal mesenchymal cells such as fibroblasts. Hyaluronan and proteins that associate with hyaluronan such as versican and tumor necrosis factor-inducible gene-6 (TSG-6) are extracellular matrix (ECM) components that promote leukocyte adhesion, accumulation, and activation. However, the factors responsible for producing this specialized ECM and its impact on inflammatory events are not well understood. In this study, we explored the role of T cells in stimulating lung fibroblasts to produce an ECM that impacts monocyte adhesion...
December 16, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27981209/crohn-s-disease-fibroblasts-overproduce-the-novel-protein-kiaa1199-to-create-proinflammatory-hyaluronan-fragments
#20
Artin Soroosh, Sami Albeiroti, Gail A West, Belinda Willard, Claudio Fiocchi, Carol A de la Motte
BACKGROUND & AIMS: Crohn's Disease (CD) is a chronic inflammatory disease of the gastrointestinal tract. Fibrosis, a serious complication of CD, occurs when activated intestinal fibroblasts deposit excessive amounts of extracellular matrix (ECM) in affected areas. A major component of the ECM is high-molecular-weight hyaluronan (HA) that, when depolymerized to low-molecular-weight fragments, becomes proinflammatory and profibrotic. Mechanisms for HA degradation are incompletely understood, but the novel protein KIAA1199 recently was discovered to degrade HA...
May 2016: Cellular and Molecular Gastroenterology and Hepatology
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