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hyaluronan matrix

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https://www.readbyqxmd.com/read/28636254/pirfenidone-reduces-subchondral-bone-loss-and-fibrosis-after-murine-knee-cartilage-injury
#1
Deva D Chan, Jun Li, Wei Luo, Dan N Predescu, Brian J Cole, Anna Plaas
Pirfenidone is an anti-inflammatory and anti-fibrotic drug that has shown efficacy in lung and kidney fibrosis. Because inflammation and fibrosis have been linked to the progression of osteoarthritis, we investigated the effects of oral Pirfenidone in a mouse model of cartilage injury, which results in chronic inflammation and joint-wide fibrosis in mice that lack hyaluronan synthase 1 (Has1(-/-) ) in comparison to wild-type. Femoral cartilage was surgically injured in wild-type and Has1(-/-) mice, and Pirfenidone was administered in food starting after 3 days...
June 21, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28629001/biomimetic-electrospun-scaffolds-from-main-extracellular-matrix-components-for-skin-tissue-engineering-application-the-role-of-chondroitin-sulfate-and-sulfated-hyaluronan
#2
Sirsendu Bhowmick, Sandra Rother, Heike Zimmermann, Poh S Lee, Stephanie Moeller, Matthias Schnabelrauch, Veena Koul, Rainer Jordan, Vera Hintze, Dieter Scharnweber
Incorporation of bioactive components like glycosaminoglycans (GAGs) into tissue engineering scaffolds, is a promising approach towards developing new generation functional biomaterial. Here, we have designed electrospun nanofibrous scaffolds made of gelatin and different concentrations of chemically sulfated or non-sulfated hyaluronan (sHA or HA) and chondroitin sulfate (CS). Evenly distributed fiber morphology was observed with no differences between varying concentrations and types of GAGs. In vitro release kinetics revealed that GAGs release is driven by diffusion...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28627617/sorafenib-controls-the-epithelial%C3%A2-mesenchymal-transition-of-ovarian-cancer-cells-via-egf-and-the-cd44%C3%A2-ha-signaling-pathway-in-a-cell-type%C3%A2-dependent-manner
#3
Ga Bin Park, Hyun-Suk Ko, Daejin Kim
Cluster of differentiation (CD) 44 and epidermal growth factor (EGF) are closely involved in cellular migration and have been used as stem cell markers. Although the hyaluronan (HA)‑binding CD44 is responsible for enhanced cellular motility, the mechanism underlying its actions in various cell types and clinical conditions have yet to be elucidated. In the present study, the multikinase inhibitor sorafenib was used to investigate the diverse effects of EGF stimulation on epithelial‑mesenchymal transition (EMT) in ovarian cancer cells using immunoblotting and reverse transcription‑polymerase chain reaction...
June 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28625420/formation-and-remodeling-of-the-brain-extracellular-matrix-in-neural-plasticity-roles-of-chondroitin-sulfate-and-hyaluronan
#4
REVIEW
Shinji Miyata, Hiroshi Kitagawa
BACKGROUND: The extracellular matrix (ECM) of the brain is rich in glycosaminoglycans such as chondroitin sulfate (CS) and hyaluronan. These glycosaminoglycans are organized into either diffuse or condensed ECM. Diffuse ECM is distributed throughout the brain and fills perisynaptic spaces, whereas condensed ECM selectively surrounds parvalbumin-expressing inhibitory neurons (PV cells) in mesh-like structures called perineuronal nets (PNNs). The brain ECM acts as a non-specific physical barrier that modulates neural plasticity and axon regeneration...
June 15, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28606795/endothelium-independent-primitive-myxoid-vascularization-creates-invertebrate-like-channels-to-maintain-blood-supply-in-optic-gliomas
#5
Matija Snuderl, Guoan Zhang, Pamela Wu, Tara S Jennings, Seema Shroff, Valerio Ortenzi, Rajan Jain, Benjamin Cohen, Jason J Reidy, Mitchell S Dushay, Jeffrey H Wisoff, David H Harter, Matthias A Karajannis, David Fenyo, Thomas A Neubert, David Zagzag
Optic gliomas are brain tumors characterized by slow growth, progressive loss of vision, and limited therapeutic options. Optic gliomas contain various amounts of myxoid matrix, which can represent most of the tumor mass. We sought to investigate biological function and protein structure of the myxoid matrix in optic gliomas to identify novel therapeutic targets. We reviewed histological features and clinical imaging properties, analyzed vasculature by immunohistochemistry and electron microscopy, and performed liquid chromatography-mass spectrometry on optic gliomas, which varied in the amount of myxoid matrix...
June 9, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28605029/the-pancreatic-cancer-microenvironment-a-true-double-agent
#6
REVIEW
Laleh G Melstrom, Marcela D Salazar, Don J Diamond
The tumor microenvironment in pancreatic cancer is a complex balance of pro- and anti-tumor components. The dense desmoplasia consists of immune cells, extracellular matrix, growth factors, cytokines, and cancer associated fibroblasts (CAF) or pancreatic stellate cells (PSC). There are a multitude of targets including hyaluronan, angiogenesis, focal adhesion kinase (FAK), connective tissue growth factor (CTGF), CD40, chemokine (C-X-C motif) receptor 4 (CXCR-4), immunotherapy, and Vitamin D. The developing clinical therapeutics will be reviewed...
June 12, 2017: Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28604766/the-deubiquitinating-enzymes-usp4-and-usp17-target-hyaluronan-synthase-2-and-differentially-affect-its-function
#7
M Mehić, V K de Sa, S Hebestreit, C-H Heldin, P Heldin
The levels of hyaluronan, a ubiquitous glycosaminoglycan prominent in the extracellular matrix, is balanced through the actions of hyaluronan-synthesizing enzymes (HAS1, 2 and 3) and degrading hyaluronidases (Hyal 1, 2, 3 and PH20). Hyaluronan accumulates in rapidly remodeling tissues, such as breast cancer, due to deregulated expression of the HAS2 gene and/or alterations of HAS2 activity. The activity of HAS2 is regulated by post-translational modifications, including ubiquitination. In order to identify deubiquitinating enzymes (DUBs) that are involved in de-ubiquitination of HAS2, a complementary (cDNA) library of 69 Flag-HA-tagged human DUBs cloned into retroviral vectors was screened in human embryonic kidney (HEK) 293T cells for their ability to de-ubiquitinate myc-tagged HAS2...
June 12, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28591491/cover-image-volume-118-number-8-august-2017
#8
Léa Gaucherand, Ben A Falk, Stephen P Evanko, Gail Workman, Christina K Chan, Thomas N Wight
Cover: The cover image, by Léa Gaucherand et al., is based on the Article Crosstalk Between T Lymphocytes and Lung Fibroblasts: Generation of a Hyaluronan-Enriched Extracellular Matrix Adhesive for Monocytes, DOI: 10.1002/jcb.25842.
August 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28582436/is-hyaluronan-deposition-in-the-stroma-of-pancreatic-ductal-adenocarcinoma-of-prognostic-significance
#9
Florian Gebauer, Marius Kemper, Guido Sauter, Peter Prehm, Udo Schumacher
BACKGROUND: Pancreatic ductal adenocarcinoma (PDAC) has a dismal prognosis and the number of PDAC-related deaths is rising. Recently the tumour stroma and in particular one of its main components, hyaluronan (HA), have attracted considerable attention as intravenous hyaluronidase treatment together with conventional chemotherapy considerably prolonged survival in HA-rich PDA patients. We therefore wanted to investigate the prognostic significance of HA deposition in PDA using both antibodies to HA and hyaluronan binding protein (HABP)...
2017: PloS One
https://www.readbyqxmd.com/read/28564597/synaptic-remodeling-depends-on-signaling-between-serotonin-receptors-and-the-extracellular-matrix
#10
Monika Bijata, Josephine Labus, Daria Guseva, Michał Stawarski, Malte Butzlaff, Joanna Dzwonek, Jenny Schneeberg, Katrin Böhm, Piotr Michaluk, Dmitri A Rusakov, Alexander Dityatev, Grzegorz Wilczyński, Jakub Wlodarczyk, Evgeni Ponimaskin
Rewiring of synaptic circuitry pertinent to memory formation has been associated with morphological changes in dendritic spines and with extracellular matrix (ECM) remodeling. Here, we mechanistically link these processes by uncovering a signaling pathway involving the serotonin 5-HT7 receptor (5-HT7R), matrix metalloproteinase 9 (MMP-9), the hyaluronan receptor CD44, and the small GTPase Cdc42. We highlight a physical interaction between 5-HT7R and CD44 (identified as an MMP-9 substrate in neurons) and find that 5-HT7R stimulation increases local MMP-9 activity, triggering dendritic spine remodeling, synaptic pruning, and impairment of long-term potentiation (LTP)...
May 30, 2017: Cell Reports
https://www.readbyqxmd.com/read/28552385/regional-distribution-of-phospholipids-in-porcine-vitreous-humor
#11
Abigail Schnepf, Marta Cecilia Yappert, Douglas Borchman
This project explores the regional phospholipid distribution in porcine vitreous humor, retina, and lens. Matrix-assisted laser desorption mass spectrometry has been used previously to image lipids, proteins, and other metabolites in retinas and lenses. However, the regional composition of phospholipids in vitreous humors is not known. To address this issue, we have applied this mass spectral method to explore the regional phospholipid distribution in porcine vitreous humor both ex-situ and in-vitro. To establish the possible source(s) of phospholipids in the vitreous humor, compositional studies of the lens and retina were also pursued...
May 25, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28549864/hyaluronan-hydrogels-with-a-low-degree-of-modification-as-scaffolds-for-cartilage-engineering
#12
Annalisa La Gatta, Giulia Ricci, Antonietta Stellavato, Marcella Cammarota, Rosanna Filosa, Agata Papa, Antonella D'Agostino, Marianna Portaccio, Ines Delfino, Mario De Rosa, Chiara Schiraldi
In the field of cartilage engineering, continuing efforts have focused on fabricating scaffolds that favor maintenance of the chondrocytic phenotype and matrix formation, in addition to providing a permeable, hydrated, microporous structure and mechanical support. The potential of hyaluronan-based hydrogels has been well established, but the ideal matrix remains to be developed. This study describes the development of hyaluronan sponges-based scaffolds obtained by lysine methyl-ester crosslinking. The reaction conditions are optimized with minimal chemical modifications to obtain materials that closely resemble elements in physiological cellular environments...
May 24, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28534077/white-matter-injury-in-the-preterm-infant-pathology-and-mechanisms
#13
REVIEW
Stephen A Back
The human preterm brain is particularly susceptible to cerebral white matter injury (WMI) that disrupts the normal progression of developmental myelination. Advances in the care of preterm infants have resulted in a sustained reduction in the severity of WMI that has shifted from more severe focal necrotic lesions to milder diffuse WMI. Nevertheless, WMI remains a global health problem and the most common cause of chronic neurological morbidity from cerebral palsy and diverse neurobehavioral disabilities. Diffuse WMI involves maturation-dependent vulnerability of the oligodendrocyte (OL) lineage with selective degeneration of late oligodendrocyte progenitors (preOLs) triggered by oxidative stress and other insults...
May 22, 2017: Acta Neuropathologica
https://www.readbyqxmd.com/read/28531976/synthesis-of-in-situ-formable-hydrogels-with-collagen-and-hyaluronan-through-facile-michael-addition
#14
Riwang Li, Zhengwei Cai, Zhiwen Li, Qian Zhang, Shuyun Zhang, Li Deng, Lu Lu, Lihua Li, Changren Zhou
To mimic the natural extracellular matrix (ECM) and to facilitate tissue regeneration, in-situ formable collagen/hyaluronan composite hydrogels were prepared by a facile approach via Michael addition reaction, with maleilated collagen (Col-MA) and thiol derivatized hyaluronan (HA-SH). The hydrogels were denoted as CHG-1, CHG-2, CHG-3 and CHG-4 by vinyl/free thiol molar ratio (f) of 1:1, 1:2, 1:3 and 1:4, respectively. Results showed that with decrease of the f values, the gelation time decreased from 43s to 15s, Young's modulus increased from 1671...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28510506/signaling-at-the-crossroads-matrix-derived-proteoglycan-and-reactive-oxygen-species-signaling
#15
Madalina-Viviana Nastase, Andrea Janicova, Malgorzata Wygrecka, Liliana Schaefer
SIGNIFICANCE: Proteoglycans (PGs), besides their structural contribution, have emerged as dynamic components that mediate a multitude of cellular events. The various roles of PGs are attributed to their structure, spatial localization, and ability to act as ligands and receptors. Reactive oxygen species (ROS) are small mediators, which are generated in physiological and pathological conditions. Besides their reactivity and ability to induce oxidative stress, a growing body of data suggests that ROS signaling is more relevant than direct radical damage in development of human pathologies...
May 16, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28493326/hyperglycaemia-and-aberrated-insulin-signalling-stimulate-tumour-progression-via-induction-of-the-extracellular-matrix-component-hyaluronan
#16
Sören Twarock, Christina Reichert, Ulrike Peters, Daniel J Gorski, Katharina Röck, Jens W Fischer
Epidemiological studies have detected a higher incidence of various tumour entities in diabetic patients. However, the underlying mechanisms remain insufficiently understood. Glucose-derived pericellular and extracellular hyaluronan (HA) promotes tumour progression and development. In our study, we tested the hypothesis that a diabetic metabolic state, characterised by hyperglycaemia and concomitant aberrant insulin signalling, stimulates tumour progression via the induction of HA synthesis. In a streptozotocin-induced diabetic nude mouse tumour xenograft model, hyperglycaemia and lack of insulin caused an increased formation of tumour-associated HA-matrix, which in turn accelerated tumour progression and neoangiogenesis...
May 10, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28488903/versican-a-subdomain-is-required-for-its-adequate-function-in-dermal-development
#17
Sonoko Hatano, Naoko Nagai, Nobuo Sugiura, Jun Tsuchimoto, Zenzo Isogai, Koji Kimata, Akinobu Ota, Sivasundaram Karnan, Yoshitaka Hosokawa, Hideto Watanabe
Versican, a large chondroitin sulfate (CS) proteoglycan, serves as a structural macromolecule of the extracellular matrix (ECM) and regulates cell behavior. We determined the function of versican in dermal development using Vcan(Δ3/Δ3) mutant mice expressing versican with deleted A-subdomain of the N-terminal G1 domain. The mutant versican showed a decreased hyaluronan (HA)-binding ability and failed to accumulate in the ECM. In the early developmental stage, Vcan(Δ3/Δ3) dermis showed a decrease in versican expression as compared with WT...
May 10, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28483644/human-mesenchymal-stem-cells-secrete-hyaluronan-coated-extracellular-vesicles
#18
Uma Thanigai Arasu, Riikka Kärnä, Kai Härkönen, Sanna Oikari, Arto Koistinen, Heikki Kröger, Chengjuan Qu, Mikko J Lammi, Kirsi Rilla
Extracellular vesicles (EVs) secreted by stem cells are potential factors mediating tissue regeneration. They travel from bone marrow stem cells into damaged tissues, suggesting that they can repair tissue injuries without directly replacing parenchymal cells. We have discovered that hyaluronan (HA) synthesis is associated with the shedding of HA-coated EVs. The aim of this study was to test whether bone marrow-derived hMSCs secrete HA-coated EVs. The EVs secreted by MSCs were isolated by differential centrifugation and characterized by nanoparticle tracking analysis...
May 5, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28482035/microvasculature-of-the-mouse-cerebral-cortex-exhibits-increased-accumulation-and-synthesis-of-hyaluronan-with-aging
#19
May J Reed, Robert B Vernon, Mamatha Damodarasamy, Christina K Chan, Thomas N Wight, Itay Bentov, William A Banks
The microvasculature of the aged brain is less dense and more vulnerable to dysfunction than that of the young brain. Brain microvasculature is supported by its surrounding extracellular matrix, which is comprised largely of hyaluronan (HA). HA is continually degraded into lower molecular weight forms that induce neuroinflammation. We examined HA associated with microvessels (MV) of the cerebral cortex of young (4 months), middle-aged (14 months), and aged (24-26 months) mice. We confirmed that the density of cortical MV decreased with age...
June 1, 2017: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/28470629/hyaluronan-thiomer-gel-matrix-mediated-healing-of-articular-cartilage-defects-in-new-zealand-white-rabbits-a-pilot-study
#20
Christoph Bauer, Vivek Jeyakumar, Eugenia Niculescu-Morzsa, Daniela Kern, Stefan Nehrer
BACKGROUND: Articular cartilage defects are limited to their regenerative potential in human adults. Our current study evaluates tissue regeneration in a surgically induced empty defect site with hyaluronan thiomer as a provisional scaffold in a gel/matrix combination without cells on rabbit models to restore tissue formation. METHODS: An osteochondral defect of 4 mm in diameter and 5 mm in depth was induced by mechanical drilling in the femoral center of the trochlea in 18 New Zealand White rabbits...
December 2017: Journal of Experimental Orthopaedics
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