keyword
MENU ▼
Read by QxMD icon Read
search

Protein Kinase

keyword
https://www.readbyqxmd.com/read/29055152/predominance-of-regorafenib-over-sorafenib-restoration-of-membrane-bound-mica-in-hepatocellular-carcinoma-cells
#1
Jun Arai, Kaku Goto, Anthony Stephanou, Yasushi Tanoue, Sayaka Ito, Ryosuke Muroyama, Yasuo Matsubara, Ryo Nakagawa, Sayuri Morimoto, Yoshimi Kaise, Lay Ahyoung Lim, Hitoshi Yoshida, Naoya Kato
BACKGROUND AND AIM: The multi-kinase inhibitor regorafenib (REG) was recently demonstrated to be effective in patients with sorafenib (SOR)-resistant hepatocellular carcinoma (HCC). Interestingly, SOR is known to enhance the accumulation of membrane-bound MHC class I polypeptide-related sequence A (mMICA) in HCC cells, and to block production of soluble MICA (sMICA), an immunological decoy. In addition, MICA is associated with HCC in patients with chronic hepatitis C. We have now compared the impact of REG and SOR on MICA in HCC cells, as well as the immunotherapeutic implications thereof...
October 21, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/29055086/acute-mitogen-activated-protein-kinase-1-2-inhibition-improves-functional-recovery-and-vascular-changes-after-ischemic-stroke-in-rat-monitored-by-9-4-t-magnetic-resonance-imaging
#2
Maryam Mostajeran, Friedrich Wetterling, Frank W Blixt, Lars Edvinsson, Saema Ansar
AIM: The aim was to evaluate the beneficial effect of early mitogen activated protein kinase (MEK)1/2 inhibition administered at a clinical relevant time-point using the transient middle cerebral artery occlusion model and a dedicated rodent magnetic resonance imaging system (9.4T) to monitor cerebrovascular changes non-invasively for two weeks. METHOD: Transient middle cerebral artery occlusion was induced in male rats for two hours followed by reperfusion. The specific MEK1/2 inhibitor U0126 was administered i...
October 21, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/29055016/sgk1-is-a-critical-component-of-an-akt-independent-pathway-essential-for-pi3k-mediated-tumor-development-and-maintenance
#3
Arturo Orlacchio, Michela Ranieri, Martina Brave, Valeria Antico Arciuch, Toni Forde, Daniela De Martino, Karen E Anderson, Phillip Hawkins, Antonio Di Cristofano
Activation of the PI3K-AKT signaling cascade is a common critical event during malignant transformation. In this study, we used thyroid gland epithelial cells and a series of genetically engineered mouse strains as model systems to demonstrate that, while necessary, AKT activation is not sufficient for PI3K-driven transformation. Instead, transformation requires the activity of the PDK1-regulated AGC family of protein kinases. In particular, SGK1 was found to be essential for proliferation and survival of thyroid cancer cells harboring PI3K-activating mutations...
October 20, 2017: Cancer Research
https://www.readbyqxmd.com/read/29055012/type-i-interferon-enhances-necroptosis-of-salmonella-typhimurium-infected-macrophages-by-impairing-antioxidative-stress-responses
#4
Nina Judith Hos, Raja Ganesan, Saray Gutiérrez, Deniz Hos, Jennifer Klimek, Zeinab Abdullah, Martin Krönke, Nirmal Robinson
Salmonella enterica serovar Typhimurium exploits the host's type I interferon (IFN-I) response to induce receptor-interacting protein (RIP) kinase-mediated necroptosis in macrophages. However, the events that drive necroptosis execution downstream of IFN-I and RIP signaling remain elusive. In this study, we demonstrate that S Typhimurium infection causes IFN-I-mediated up-regulation of the mitochondrial phosphatase Pgam5 through RIP3. Pgam5 subsequently interacts with Nrf2, which sequesters Nrf2 in the cytosol, thereby repressing the transcription of Nrf2-dependent antioxidative genes...
October 20, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/29054976/regulation-of-insulin-like-growth-factor-receptors-by-ubiquilin1
#5
Zimple Kurlawala, Rain Dunaway, Parag P Shah, Julie A Gosney, Leah J Siskind, Brian P Ceresa, Levi J Beverly
Insulin-like growth factor-1 receptor (IGF1R) is a receptor tyrosine kinase that mediates growth, proliferation and survival. Dysregulation of IGF pathway contributes to initiation, progression and metastasis of cancer and is also involved in diseases of glucose metabolism, such as Diabetes. We have identified Ubiquilin1 (UBQLN1) as a novel interaction partner of IGF1R, IGF2R and Insulin Receptor. UBQLN family of proteins have been studied primarily in the context of protein quality control and in the field of neurodegenerative disorders...
October 20, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/29054929/mitogen-and-stress-activated-protein-kinase-1-is-required-for-gonadotropin-releasing-hormone-mediated-activation-of-gonadotropin-%C3%AE-subunit-expression
#6
Majd Haj, Andrea Wijeweera, Sergei Rudnizky, Jack Taunton, Lilach Pnueli, Philippa Melamed
Pituitary gonadotropin hormones are regulated by gonadotropin-releasing hormone (GnRH) via MAPK signaling pathways which stimulate gene transcription of the common α-subunit (Cga) and the hormone-specific β-subunits of gonadotropin. We have reported previously that GnRH-induced activities at these genes include various histone modifications, but we did not examine histone phosphorylation. This modification adds a negative charge to residues of the histone tails that interact with the negatively charged DNA, is associated with closed chromatin during mitosis, but is increased at certain genes for transcriptional activation...
October 20, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29054927/the-c-terminal-region-of-a-kinase-anchor-protein-350-akap350a-enables-formation-of-microtubule-nucleation-centers-and-interacts-with-pericentriolar-proteins
#7
Elena Kolobova, Joseph T Roland, Lynne A Lapierre, Janice A Williams, Twila A Mason, James R Goldenring
Microtubules in animal cells assemble (nucleate) from both the centrosome and the cis-Golgi cisternae. A-kinase anchoring protein 350 KDa (AKAP350A, also called AKAP450/CG-NAP/AKAP9) is a large scaffolding protein located at both the centrosome and Golgi apparatus. Previous findings have suggested that AKAP350 is important for microtubule dynamics at both locations, but how this scaffolding protein assembles microtubule nucleation machinery is unclear. Here, we found that overexpression of the C-terminal third of AKAP350A, EGFP-AKAP350A(2691-3907), induces the formation of multiple microtubule-nucleation centers (MTNCs)...
October 20, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29054922/protein-turnover-measurements-in-human-serum-by-serial-immunoaffinity-lc-ms-ms
#8
Vahid Farrokhi, Xiaoying Chen, Hendrik Neubert
BACKGROUND: The half-life of target proteins is frequently an important parameter in mechanistic pharmacokinetic and pharmacodynamic (PK/PD) modeling of biotherapeutics. Clinical studies for accurate measurement of physiologically relevant protein turnover can reduce the uncertainty in PK/PD model-based predictions, for example, of the therapeutic dose and dosing regimen in first-in-human clinical trials. METHODS: We used a targeted mass spectrometry work flow based on serial immunoaffinity enrichment of multiple human serum proteins from a [5,5,5-(2)H3]-L-leucine tracer pulse-chase study in healthy volunteers...
October 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/29054855/h-ras-deletion-protects-against-angiotensin-ii-induced-arterial-hypertension-and-cardiac-remodeling-through-protein-kinase-g-i%C3%AE-pathway-activation
#9
Paloma Martín-Sánchez, Alicia Luengo, Mercedes Griera, María Jesús Orea, Marina López-Olañeta, Antonio Chiloeches, Enrique Lara-Pezzi, Sergio de Frutos, Manuel Rodríguez-Puyol, Laura Calleros, Diego Rodríguez-Puyol
Ras proteins regulate cell survival, growth, differentiation, blood pressure, and fibrosis in some organs. We have demonstrated that H-ras gene deletion produces mice hypotension via a soluble guanylate cyclase-protein kinase G (PKG)-dependent mechanism. In this study, we analyzed the consequences of H-ras deletion on cardiac remodeling induced by continuous angiotensin II (AngII) infusion and the molecular mechanisms implied. Left ventricular posterior wall thickness and mass and cardiomyocyte cross-sectional area were similar between AngII-treated H-Ras knockout (H-ras(-/-) ) and control wild-type (H-ras(+/+) ) mice, as were extracellular matrix protein expression...
October 20, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29054824/4-hydroxycinnamic-acid-protects-mice-from-cigarette-smoke-induced-pulmonary-inflammation-via-mapk-pathways
#10
Sung-Hyeuk Park, Je-Won Ko, Na-Rae Shin, Dong-Ho Shin, Young-Kwon Cho, Chang-Seob Seo, Jong-Choon Kim, Joong-Sun Kim, In-Sik Shin
Cigarette smoke (CS) is the main etiological cause of chronic obstructive pulmonary disease, the prevalence of which has continuously increased in recent years. 4-Hydroxycinnamic acid (HA) is a plant phenolic acid that has anti-inflammatory activities. In this study, we explored the therapeutic effects of HA on airway inflammation caused by CS and lipopolysaccharide (LPS) in mice. The animals received 1 h of CS exposure for 7 days and intranasal instillation of LPS on day 4. HA (10 and 20 mg/kg) was administered to animals via oral gavage 1 h before CS exposure...
October 17, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29054429/surface-chemistry-induces-mitochondria-mediated-apoptosis-of-breast-cancer-cells-via-pten-pi3k-akt-signaling-pathway
#11
Jing Zhang, Li Li, Yueting Peng, Yu Chen, Xiaoying Lv, Shun Li, Xiang Qin, Hong Yang, Chunhui Wu, Yiyao Liu
Tumor cell can be significantly influenced by various chemical groups of the extracellular matrix proteins. However, the underlying molecular mechanisms involved in the interaction between cancer cells and functional groups in the extracellular matrix remain unknown. Using chemically modified surfaces with biological functional groups (CH3, NH2, OH), it was found that hydrophobic surfaces modified with CH3 and NH2 suppressed cell proliferation and induced the number of apoptotic cells. Mitochondrial dysfunction, cytochrome c release, Bax upregulation, cleaved caspase-3 and PARP, and Bcl-2 downregulation indicated that hydrophobic surfaces with CH3 and NH2 triggered the activation of intrinsic apoptotic signaling pathway...
October 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29054405/standardized-extract-of-bacopa-monnieri-cdri-08-effect-on-germ-cell-dynamics-and-possible-mechanisms-of-its-beneficial-action-on-spermatogenesis-and-sperm-quality-in-male-mice
#12
Shishir Kumar Patel, Shilpi Singh, Shio Kumar Singh
Bacopa monnieri (BM) is used in traditional medicine as nerve tonic. We have recently shown that CDRI-08, a standardized extract of BM, improves testicular functions and epididymal sperm quality in Parkes (P) mice. The aim of the present study was to investigate the effect of CDRI-08 on germ cell dynamics and mechanisms of its action on spermatogenesis and sperm quality in P mice, and to determine the chemical profile of the extract. CDRI-08 (40 and 80 mg/kg body weight) was orally administered to male mice for 28 days...
October 17, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29054375/atr-chk1-inhibitors-and-cancer-therapy
#13
REVIEW
Zhaojun Qiu, Nancy L Oleinick, Junran Zhang
The cell cycle checkpoint proteins ataxia-telangiectasia-mutated-and-Rad3-related kinase (ATR) and its major downstream effector checkpoint kinase 1 (CHK1) prevent the entry of cells with damaged or incompletely replicated DNA into mitosis when the cells are challenged by DNA damaging agents, such as radiation therapy (RT) or chemotherapeutic drugs, that are the major modalities to treat cancer. This regulation is particularly evident in cells with a defective G1 checkpoint, a common feature of cancer cells, due to p53 mutations...
October 17, 2017: Radiotherapy and Oncology: Journal of the European Society for Therapeutic Radiology and Oncology
https://www.readbyqxmd.com/read/29054256/drug-target-interaction-prediction-a-bayesian-ranking-approach
#14
Ladislav Peska, Krisztian Buza, Júlia Koller
BACKGROUND AND OBJECTIVE: In silico prediction of drug-target interactions (DTI) could provide valuable information and speed-up the process of drug repositioning - finding novel usage for existing drugs. In our work, we focus on machine learning algorithms supporting drug-centric repositioning approach, which aims to find novel usage for existing or abandoned drugs. We aim at proposing a per-drug ranking-based method, which reflects the needs of drug-centric repositioning research better than conventional drug-target prediction approaches...
December 2017: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/29054108/increase-in-pro-apoptotic-activity-of-inhibitory-pas-domain-protein-via-phosphorylation-by-mk2
#15
Shuya Kasai, Mary Jane Elizabeth Richardson, Satoru Torii, Ken-Ichi Yasumoto, Hiroki Shima, Kazuhiko Igarashi, Ken Itoh, Kazuhiro Sogawa, Kazutaka Murayama
Inhibitory PAS domain protein (IPAS) is a bifunctional protein that downregulates hypoxic gene expression and exerts pro-apoptotic activity by preventing pro-survival activity of Bcl-xL and its related factors. Pro-apoptotic activity of IPAS is attenuated by the activation of the PINK1-Parkin pathway, and involved in neuronal degeneration in an experimental mouse model of Parkinson's disease. The current study shows that phosphorylation of IPAS at Ser184 by MAPK-activated protein kinase 2 (MK2 or MAPKAPK2) enhances the pro-apoptotic function of IPAS...
October 20, 2017: FEBS Journal
https://www.readbyqxmd.com/read/29054072/cysteine-perthiosulfenic-acid-cys-ssoh-a-novel-intermediate-in-thiol-based-redox-signaling
#16
David E Heppner, Milena Hristova, Tomoaki Ida, Ana Mijuskovic, Christopher M Dustin, Virág Bogdándi, Jon M Fukuto, Tobias P Dick, Péter Nagy, Jianing Li, Takaaki Akaike, Albert van der Vliet
The reversible oxidation of protein cysteine residues (Cys-SH) is a key reaction in cellular redox signaling involving initial formation of sulfenic acids (Cys-SOH), which are commonly detected using selective dimedone-based probes. Here, we report that significant portions of dimedone-tagged proteins are susceptible to cleavage by DTT reflecting the presence of perthiosulfenic acid species (Cys-SSOH) due to similar oxidation of hydropersulfides (Cys-SSH), since Cys-S-dimedone adducts are stable toward DTT...
October 9, 2017: Redox Biology
https://www.readbyqxmd.com/read/29054015/synergistic-effect-of-copper-and-arsenic-upon-oxidative-stress-inflammation-and-autophagy-alterations-in-brain-tissues-of-gallus-gallus
#17
Xiao Sun, Jinglun Li, Hongjing Zhao, Yu Wang, Juanjuan Liu, Yizhi Shao, Yuan Xue, Mingwei Xing
Arsenic or copper is one of the most highly toxic pollution that can cause dysfunction to brains, however, the exact mechanism remains unclear. The aim of the study is to investigate the mechanisms of arsenic or/and copper-induced oxidative stress, inflammation and autophagy in chicken brains and elucidate the interactions between arsenic and copper. A total of 72 1-day-old Hy-line chickens were divided into four groups (18 chickens per group) treated with 30mg/kg arsenic trioxide (As2O3) or/and 300mg/kg copper sulfate (CuSO4) for 12weeks...
October 12, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29053988/intermedin-reduces-neointima-formation-by-regulating-vascular-smooth-muscle-cell-phenotype-via-camp-pka-pathway
#18
Qing Zhu, Xian-Qiang Ni, Wei-Wei Lu, Jin-Sheng Zhang, Jin-Ling Ren, Di Wu, Yao Chen, Lin-Shuang Zhang, Yan-Rong Yu, Chao-Shu Tang, Yong-Fen Qi
BACKGROUND AND AIMS: Vascular smooth muscle cell (VSMC) dedifferentiation contributes to neointima formation, which results in various vascular disorders. Intermedin (IMD), a cardiovascular paracrine/autocrine polypeptide, is involved in maintaining circulatory homeostasis. However, whether IMD protects against neointima formation remains largely unknown. The purpose of this study is to investigate the role of IMD in neointima formation and the possible mechanism. METHODS: IMD1-53 (100ng/kg/h) or saline water was used on rat carotid-artery balloon-injury model...
October 9, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/29053970/mtorc1-activator-slc38a9-is-required-to-efflux-essential-amino-acids-from-lysosomes-and-use-protein-as-a-nutrient
#19
Gregory A Wyant, Monther Abu-Remaileh, Rachel L Wolfson, Walter W Chen, Elizaveta Freinkman, Laura V Danai, Matthew G Vander Heiden, David M Sabatini
The mTORC1 kinase is a master growth regulator that senses many environmental cues, including amino acids. Activation of mTORC1 by arginine requires SLC38A9, a poorly understood lysosomal membrane protein with homology to amino acid transporters. Here, we validate that SLC38A9 is an arginine sensor for the mTORC1 pathway, and we uncover an unexpectedly central role for SLC38A9 in amino acid homeostasis. SLC38A9 mediates the transport, in an arginine-regulated fashion, of many essential amino acids out of lysosomes, including leucine, which mTORC1 senses through the cytosolic Sestrin proteins...
October 19, 2017: Cell
https://www.readbyqxmd.com/read/29053969/pebp1-wardens-ferroptosis-by-enabling-lipoxygenase-generation-of-lipid-death-signals
#20
Sally E Wenzel, Yulia Y Tyurina, Jinming Zhao, Claudette M St Croix, Haider H Dar, Gaowei Mao, Vladimir A Tyurin, Tamil S Anthonymuthu, Alexandr A Kapralov, Andrew A Amoscato, Karolina Mikulska-Ruminska, Indira H Shrivastava, Elizabeth M Kenny, Qin Yang, Joel C Rosenbaum, Louis J Sparvero, David R Emlet, Xiaoyan Wen, Yoshinori Minami, Feng Qu, Simon C Watkins, Theodore R Holman, Andrew P VanDemark, John A Kellum, Ivet Bahar, Hülya Bayır, Valerian E Kagan
Ferroptosis is a form of programmed cell death that is pathogenic to several acute and chronic diseases and executed via oxygenation of polyunsaturated phosphatidylethanolamines (PE) by 15-lipoxygenases (15-LO) that normally use free polyunsaturated fatty acids as substrates. Mechanisms of the altered 15-LO substrate specificity are enigmatic. We sought a common ferroptosis regulator for 15LO. We discovered that PEBP1, a scaffold protein inhibitor of protein kinase cascades, complexes with two 15LO isoforms, 15LO1 and 15LO2, and changes their substrate competence to generate hydroperoxy-PE...
October 19, 2017: Cell
keyword
keyword
36678
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"