keyword
MENU ▼
Read by QxMD icon Read
search

Protein Kinase

keyword
https://www.readbyqxmd.com/read/29668110/phenylbutyl-isoselenocyanate-induces-reactive-oxygen-species-to-inhibit-androgen-receptor-and-to-initiate-p53-mediated-apoptosis-in-lncap-prostate-cancer-cells
#1
Wei Wu, Deepkamal Karelia, Kartick Pramanik, Shantu G Amin, Arun K Sharma, Cheng Jiang, Junxuan Lu
Previous studies have established the in vivo bioavailability and efficacious dosages of phenylbutyl isoselenocyanate (ISC-4), a selenium-substituted isothiocyanate, against mouse xenograft models of human melanoma and colorectal cancer. To explore its potential attributes against prostate cancer, we treated human LNCaP prostate cancer cells with ISC-4 and examined their apoptosis responses, and interrogated the signaling mechanisms through pharmacological and siRNA knockdown approaches. Our results show that ISC-4 was more potent at inducing apoptosis than its sulfur analog phenylbutyl isothiocyanate (PBITC) without suppressing protein kinase AKT Ser473 phosphorylation...
April 18, 2018: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/29668051/evidence-for-a-specific-and-critical-role-of-mitogen-activated-protein-kinase-20-in-uni-to-binucleate-transition-of-microgametogenesis-in-tomato
#2
Lifei Chen, Dandan Yang, Youwei Zhang, Limin Wu, Yaoyao Zhang, Lei Ye, Changtian Pan, Yanjun He, Li Huang, Yong-Ling Ruan, Gang Lu
Mitogen-activated protein kinases (MAPKs) regulate diverse aspects of plant growth. However, their potential role in reproductive development remains elusive. Here, we discovered an unique role of SlMPK20, a plant-specific group D MAPK, in pollen development in tomato. RNAi-mediated suppression of SlMPK20 or its knockout using CRISPR/Cas9 significantly reduced or completely abolished pollen viability, respectively, with no effects on maternal fertility. Cell biology and gene expression analyses established that SlMPK20 exerts its role specifically at the uni-to-binucleate transition during microgametogenesis...
April 18, 2018: New Phytologist
https://www.readbyqxmd.com/read/29667925/massive-over-representation-of-solute-binding-proteins-sbps-from-the-tripartite-tricarboxylate-transporter-ttt-family-in-the-genome-of-the-%C3%AE-proteobacterium-rhodoplanes-sp-z2-yc6860
#3
Leonardo T Rosa, Vicki Springthorpe, Matheus E Bianconi, Gavin H Thomas, David J Kelly
Lineage-specific expansion (LSE) of protein families is a widespread phenomenon in many eukaryotic genomes, but is generally more limited in bacterial genomes. Here, we report the presence of 434 genes encoding solute-binding proteins (SBPs) from the tripartite tricarboxylate transporter (TTT) family, within the 8.2 Mb genome of the α-proteobacterium Rhodoplanes sp. Z2-YC6860, a gene family over-representation of unprecedented abundance in prokaryotes. Representing over 6 % of the total number of coding sequences, the SBP genes are distributed across the whole genome but are found rarely in low-GC islands, where the gene density for this family is much lower...
April 18, 2018: Microbial Genomics
https://www.readbyqxmd.com/read/29667914/inactivation-of-map3k7-in-foxd1-expressing-cells-results-in-loss-of-mesangial-pdgfr%C3%AE-and-juvenile-kidney-scarring
#4
Michele J Karolak, Justin A Guay, Leif Oxburgh
Transforming growth factor beta (TGFβ) plays a central role in renal scarring, controlling extracellular matrix deposition by interstitial cells and mesangial cells. TGFβ signals through Smad and mitogen activated protein kinase (MAPK) pathways. To understand the role of MAPK in interstitial and mesangial cells, we genetically inactivated TGFβ-activated kinase 1 (Map3k7) using Foxd1+/cre. Embryonic kidney development was unperturbed in mutants, but spontaneous scarring of the kidney ensued during the first postnatal week, with retention of embryonic nephrogenic rests and accumulation of collagen IV in the mesangium...
April 18, 2018: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29667822/ypaoamides-b-and-c-linear-lipopeptides-from-an-okeania-sp-marine-cyanobacterium
#5
Kosuke Sueyoshi, Miki Yamada, Aki Yamano, Kaori Ozaki, Shimpei Sumimoto, Arihiro Iwasaki, Kiyotake Suenaga, Toshiaki Teruya
Two new pyrrolinone-containing lipopeptides, ypaoamides B (1) and C (2), were isolated from an Okeania sp. marine cyanobacterium collected in Okinawa. Their structures were determined by spectroscopic analysis and Marfey's analysis of acid hydrolysates. Ypaoamides B (1) and C (2) stimulated glucose uptake in cultured rat L6 myotubes. In particular, ypaoamide B (1) showed potent activity and activated AMP-activated protein kinase.
April 18, 2018: Journal of Natural Products
https://www.readbyqxmd.com/read/29667773/benzyl-isothiocyanate-attenuates-the-hydrogen-peroxide-induced-interleukin-13-expression-through-glutathione-s-transferase-p-induction-in-t-lymphocytic-leukemia-cells
#6
Yue Tang, Sho Naito, Naomi Abe-Kanoh, Seiji Ogawa, Shu Yamaguchi, Beiwei Zhu, Yoshiyuki Murata, Yoshimasa Nakamura
We investigated the effect of benzyl isothiocyanate (BITC) on the hydrogen peroxide-induced gene expression of a T-helper-2 cytokine, interleukin (IL)-13, in T lymphocytic leukemia Jurkat cells. The 24-h pretreatment of BITC significantly inhibited the IL-13 expression enhanced by hydrogen peroxide. Although the BITC pretreatment did not change the enhanced level of the phosphorylated c-Jun N-terminal kinase (JNK), it significantly inhibited the nuclear translocation of c-Jun induced by hydrogen peroxide. BITC also increased the protein expression of glutathione S-transferase (GST) isozymes, GSTP1/2, as well as the total GST activity...
April 18, 2018: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/29667627/propofol-inhibits-hela-cells-by-impairing-autophagic-flux-via-amp-activated-protein-kinase-ampk-activation-and-endoplasmic-reticulum-stress-regulated-by-calcium
#7
Xi Chen, Kai Li, Guoqing Zhao
BACKGROUND Propofol has antitumor effects against various cancers. However, the mechanism of action of propofol in HeLa human cervical cancer cells has not been elucidated. MATERIAL AND METHODS We treated HeLa human cervical cancer cells with different concentrations of propofol. Cell viability was evaluated with Cell Counting Kit-8 and apoptosis was analyzed by annexin V-fluorescein isothiocyanate and propidium iodide staining and flow cytometry. Autophagosome formation was evaluated based on microtubule-associated protein light chain (LC)3 conversion and light chain 3 puncta formation...
April 18, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29667548/flavonoids-and-platelet-derived-thrombotic-disorders
#8
Jose J Lopez, Mohammed El Haouari, Isaac Jardin, Nieves Alonso, Sergio Regodon, Raquel Diez Bello, Pedro Cosme Redondo, Juan A Rosado
Thrombotic disorders are characterized by an increase in the probability of the formation of unnecessary thrombi that might be due to the activation of the coagulation cascade or the circulating platelets. Platelets or thrombocytes play an essential role in hemostasis but abnormal platelet function leads to the development of a number of cardiovascular complications, including thrombotic disorders. Under pathological conditions, platelets are associated with the development of different thrombotic disorders, including atherosclerosis, arterial thrombosis and stroke, deep venous thrombosis and pulmonary embolism; therefore, platelets are the target of a number of anti-thrombotic strategies...
April 17, 2018: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29667328/insights-into-the-regulation-of-cbf-cold-signaling-in-plants
#9
REVIEW
Jingyan Liu, Yiting Shi, Shuhua Yang
Cold temperatures, a major abiotic stress, threaten the growth and development of plants worldwide. To cope with this adverse environmental cue, plants from temperate climates have evolved an array of sophisticated mechanisms to acclimate to cold periods, increasing their ability to tolerate freezing stress. Over the last decade, significant progress has been made in determining the molecular mechanisms underpinning cold acclimation following the identification of several pivotal components, including candidates for cold sensors, protein kinases, and transcription factors...
April 18, 2018: Journal of Integrative Plant Biology
https://www.readbyqxmd.com/read/29666959/carney-complex-a-case-with-thyroid-follicular-adenoma-without-a-prkar1a-mutation
#10
Shinji Hattori, Yukou Yamane, Ryoichi Shimomura, Yuki Uchida, Nobuhiko Toyota, Yoshio Miura, Setsujyo Shiota, Yoshitsugu Tajima
BACKGROUND: Carney complex (CNC) is a very rare disease. Although thyroid lesions are included in the diagnostic criteria for CNC, they are an infrequent occurrence. CASE PRESENTATION: The patient was a 69-year-old woman who had undergone the removal of a left atrial myxoma 10 years earlier, at the age of 59. At the time of the operation, thyroid ultrasonography (US) revealed multiple hypoechoic nodules. Thyroid scintigraphy revealed an increased uptake of 99m Tc in these lesions, which was consistent with toxic multinodular goiter, and she was diagnosed with CNC...
April 17, 2018: Surgical Case Reports
https://www.readbyqxmd.com/read/29666899/altered-mitochondrial-bioenergetics-and-ultrastructure-in-the-skeletal-muscle-of-young-adults-with-type-1-diabetes
#11
Cynthia M F Monaco, Meghan C Hughes, Sofhia V Ramos, Nina E Varah, Christian Lamberz, Fasih A Rahman, Chris McGlory, Mark A Tarnopolsky, Matthew P Krause, Robert Laham, Thomas J Hawke, Christopher G R Perry
AIMS/HYPOTHESIS: A comprehensive assessment of skeletal muscle ultrastructure and mitochondrial bioenergetics has not been undertaken in individuals with type 1 diabetes. This study aimed to systematically assess skeletal muscle mitochondrial phenotype in young adults with type 1 diabetes. METHODS: Physically active, young adults (men and women) with type 1 diabetes (HbA1c 63.0 ± 16.0 mmol/mol [7.9% ± 1.5%]) and without type 1 diabetes (control), matched for sex, age, BMI and level of physical activity, were recruited (n = 12/group) to undergo vastus lateralis muscle microbiopsies...
April 18, 2018: Diabetologia
https://www.readbyqxmd.com/read/29666854/casein-glycomacropeptide-hydrolysates-inhibit-pge2-production-and-cox2-expression-in-lps-stimulated-raw-264-7-macrophage-cells-via-akt-mediated-nf-%C3%AE%C2%BAb-and-mapk-pathways
#12
Tiange Li, Dongxiao Gao, Min Du, Xue Cheng, Xueying Mao
A casein glycomacropeptide hydrolysate (GMPH) was found to possess inhibitory activity against lipopolysaccharide (LPS)-induced inflammatory response in our previous study. In the current study, the inhibitory effect and the underlying molecular mechanism of GMPH on inflammatory response in LPS-stimulated RAW264.7 macrophages were further investigated. Results showed that GMPH significantly suppressed LPS-induced intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) production. GMPH reduced the production of prostaglandin E2 (PEG2) and the expression of cyclooxygenase-2 (COX-2) and cytosolic phospholipase A2 (cPLA2) in LPS-stimulated macrophages...
April 18, 2018: Food & Function
https://www.readbyqxmd.com/read/29666781/role-of-muramyl-dipeptide-in-lipopolysaccharide-mediated-biological-activity-and-osteoclast-activity
#13
REVIEW
Hideki Kitaura, Masahiko Ishida, Keisuke Kimura, Haruki Sugisawa, Akiko Kishikawa, Kazuhiro Shima, Saika Ogawa, Jiawei Qi, Wei-Ren Shen
Lipopolysaccharide (LPS) is an endotoxin and bacterial cell wall component that is capable of inducing inflammation and immunological activity. Muramyl dipeptide (MDP), the minimal essential structural unit responsible for the immunological activity of peptidoglycans, is another inflammation-inducing molecule that is ubiquitously expressed by bacteria. Several studies have shown that inflammation-related biological activities were synergistically induced by interactions between LPS and MDP. MDP synergistically enhances production of proinflammatory cytokines that are induced by LPS exposure...
2018: Analytical Cellular Pathology (Amsterdam)
https://www.readbyqxmd.com/read/29666759/phosphoproteomic-insights-into-processes-influenced-by-the-kinase-like-protein-dia1-c3orf58
#14
Agnieszka Hareza, Magda Bakun, Bianka Świderska, Małgorzata Dudkiewicz, Alicja Koscielny, Anna Bajur, Jacek Jaworski, Michał Dadlez, Krzysztof Pawłowski
Many kinases are still 'orphans,' which means knowledge about their substrates, and often also about the processes they regulate, is lacking. Here, DIA1/C3orf58, a member of a novel predicted kinase-like family, is shown to be present in the endoplasmic reticulum and to influence trafficking via the secretory pathway. Subsequently, DIA1 is subjected to phosphoproteomics analysis to cast light on its signalling pathways. A liquid chromatography-tandem mass spectrometry proteomic approach with phosphopeptide enrichment is applied to membrane fractions of DIA1-overexpressing and control HEK293T cells, and phosphosites dependent on the presence of DIA1 are elucidated...
2018: PeerJ
https://www.readbyqxmd.com/read/29666673/pan-cdk-inhibition-augments-cisplatin-lethality-in-nasopharyngeal-carcinoma-cell-lines-and-xenograft-models
#15
Nicholas L Syn, Pei Li Lim, Li Ren Kong, Lingzhi Wang, Andrea Li-Ann Wong, Chwee Ming Lim, Thomas Kwok Seng Loh, Gerhard Siemeister, Boon Cher Goh, Wen-Son Hsieh
In addition to their canonical roles in regulating cell cycle transition and transcription, cyclin-dependent kinases (CDKs) have been shown to coordinate DNA damage response pathways, suggesting a rational pairing of CDK inhibitors with genotoxic chemotherapeutic agents in the treatment of human malignancies. Here, we report that roniciclib (BAY1000394), a potent pan-CDK inhibitor, displays promising anti-neoplastic activity as a single agent and potentiates cisplatin lethality in preclinical nasopharyngeal carcinoma (NPC) models...
2018: Signal Transduction and Targeted Therapy
https://www.readbyqxmd.com/read/29666569/inhibitory-effects-of-betulinic-acid-on-lps-induced-neuroinflammation-involve-m2-microglial-polarization-via-camkk%C3%AE-dependent-ampk-activation
#16
Chuwen Li, Chao Zhang, Hefeng Zhou, Yu Feng, Fan Tang, Maggie P M Hoi, Chengwei He, Dan Ma, Chao Zhao, Simon M Y Lee
In response to the microenvironment, microglia may polarize into either an M1 pro-inflammatory phenotype, exacerbating neurotoxicity, or an M2 anti-inflammatory phenotype, conferring neuroprotection. Betulinic acid (BA) is a naturally pentacyclic triterpenoid with considerable anti-inflammatory properties. Here, we aim to investigate the potential effects of BA on microglial phenotype polarization and to reveal the underlying mechanisms of action. First, we confirmed that BA promoted M2 polarization and inhibited M1 polarization in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells...
2018: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29666506/insights-into-aluminum-tolerance-pathways-in-stylosanthes-as-revealed-by-rna-seq-analysis
#17
Caode Jiang, Lusheng Liu, Xiaofeng Li, Rongrong Han, Yunmin Wei, Yongxiong Yu
Stylo has a great potential for Al3+ resistance in acidic soils through secretion of citrate from the roots. To get insight into the molecular mechanisms responsible, transcriptomic changes were investigated in the roots after treatment with T01 (-Al3+ , pH6.0), T02 (-Al3+ , pH4.3) and T03 (50 µM AlCl3 , pH4.3). In total, 83,197 unigenes generated from 130,933 contigs were obtained. Of them, 282, 148 and 816 differentially expressed unigenes (DEGs) were revealed in T01_vs_T02, T02_vs_T03 and T01_vs_T03 comparison, respectively (FDR < 0...
April 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29666472/caspase-8-deficiency-in-mouse-embryos-triggers-chronic-ripk1-dependent-activation-of-inflammatory-genes-independently-of-ripk3
#18
Tae-Bong Kang, Ju-Seong Jeong, Seung-Hoon Yang, Andrew Kovalenko, David Wallach
Deletion of the Casp8 gene in epithelial tissues of mice results in severe inflammatory pathologies. Its ubiquitous deletion, or its specific deletion in endothelial cells, results in intrauterine death associated with capillary damage. These pathologies are all preventable by co-deletion of Casp8 and the genes encoding either the RIPK1 or the RIPK3 protein kinase. Since activation of RIPK3 in Caspase-8-deficient cells can trigger necroptotic cell death, and since RIPK1 can activate RIPK3, it is widely assumed that the inflammatory states resulting from Caspase-8 deficiency occur as a consequence of RIPK3-induced necroptosis...
April 17, 2018: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29666369/lithium-associated-transcriptional-regulation-of-crmp1-in-patient-derived-olfactory-neurons-and-symptom-changes-in-bipolar-disorder
#19
Charlee K McLean, Soumya Narayan, Sandra Y Lin, Narayan Rai, Youjin Chung, MariaMananita S Hipolito, Nicola G Cascella, John I Nurnberger, Koko Ishizuka, Akira S Sawa, Evaristus A Nwulia
There is growing evidence that lithium used in the treatment of bipolar disorder (BD) affects molecular targets that are involved in neuronal growth, survival, and maturation, but it remains unclear if neuronal alterations in any of these molecules predict specific symptom changes in BD patients undergoing lithium monotherapy. The goals of this study were to (a) determine which molecular changes in the olfactory neurons of symptomatic patients receiving lithium are associated with antimanic or antidepressant response, and (b) uncover novel intraneuronal regulatory mechanisms of lithium therapy...
April 18, 2018: Translational Psychiatry
https://www.readbyqxmd.com/read/29666307/interleukin-2-shapes-the-cytotoxic-t-cell-proteome-and-immune-environment-sensing-programs
#20
Christina M Rollings, Linda V Sinclair, Hugh J M Brady, Doreen A Cantrell, Sarah H Ross
Interleukin-2 (IL-2) and Janus kinases (JAKs) regulate transcriptional programs and protein synthesis to promote the differentiation of effector CD8+ cytotoxic T lymphocytes (CTLs). Using high-resolution mass spectrometry, we generated an in-depth characterization of how IL-2 and JAKs configure the CTL proteome to control CTL function. We found that IL-2 signaling through JAK1 and JAK3 (JAK1/3) increased the abundance of a key subset of proteins to induce the accumulation of critical cytokines and effector molecules in T cells...
April 17, 2018: Science Signaling
keyword
keyword
36678
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"