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https://www.readbyqxmd.com/read/29689065/effect-of-monotherapy-with-darunavir-cobicistat-on-viral-load-and-semen-quality-of-hiv-1-patients
#1
Miguel A López-Ruz, Miguel A López-Zúñiga, María Carmen Gonzalvo, Antonio Sampedro, Juan Pasquau, Carmen Hidalgo, Javier Rosario, Jose Antonio Castilla
Many patients previously using darunavir/ritonavir (DRV/r) (800/100mg) have switched to darunavir/cobicistat (DRV/C) (800/150 mg) either as part of triple therapy (ART) or as monotherapy with DRV (mDRV). The latter approach continues to be used in some countries for patients receiving long-term treatment. However, to date, the behaviour of DRV/C in the seminal compartment has not been analysed. This study explores how the combination behaves in monotherapy, with respect to the control of viral load and seminal quality...
2018: PloS One
https://www.readbyqxmd.com/read/29688289/testicular-aging-an-overview-of-ultrastructural-cellular-and-molecular-alterations
#2
Joana Santiago, Joana V Silva, Marco G Alves, Pedro F Oliveira, Margarida Fardilha
The trend in parenthood at an older age is increasing for both men and women in developed countries, raising concerns about the reproductive ability and the consequences for the offspring's health. While reproductive activity in women stops with menopause, a complete cessation of the reproductive potential does not occur in men. Although several studies have been published on the effects of aging on semen parameters and spermatozoa DNA integrity, literature on impact of aging on the testis, particularly cellular and molecular alterations, has been, so far, limited and controversial...
April 21, 2018: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/29688091/molecular-detection-of-hhv1-5-aav-and-hpv-in-semen-specimens-and-their-impact-on-male-fertility
#3
Emad Behboudi, Talat Mokhtari-Azad, Jila Yavarian, Nastaran Ghavami, Seyed Mahmood Seyed Khorrami, Farhad Rezaei, Javad Charostad, Somayeh Shatizadeh Malekshahi, Nazanin Zahra Shafiei-Jandaghi
Viral infections have been considered as possible destructive factors that influence male fertility. The aim of this study was to determine the prevalence of human herpes viruses 1-5 (HHV1-5), adeno associated virus (AAV) and human papilloma virus (HPV) in semen and whether these influence semen quality. DNA extraction was performed using phenol-chloroform protocol, then three different nested-PCRs were done to detect HHV1-5, AAV and HPV DNAs in the semen samples. Of 145 samples, 66 (45.5%) were positive at least for one of the viruses...
April 24, 2018: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/29684825/exposure-to-polybrominated-diphenyl-ethers-and-phthalates-in-healthy-men-living-in-the-greater-montreal-area-a-study-of-hormonal-balance-and-semen-quality
#4
Océane Albert, Jonathan Y Huang, Katarina Aleksa, Barbara F Hales, Cynthia G Goodyer, Bernard Robaire, Jonathan Chevrier, Peter Chan
Studies investigating the associations between exposure of young men to polybrominated diphenyl ethers (PBDEs) or phthalates and hormone levels or semen quality have produced inconsistent results. Our goal was to investigate the association of exposure to PBDEs or phthalate metabolites with changes in markers of thyroid (TSH, free T3 and free T4) and reproductive function (sperm concentrations, motility, and quality; serum LH and testosterone) in 153 healthy young men from the greater Montreal area. Using covariate-adjusted models, we found that each 10-fold increase in BDE-47 was associated with lower TSH levels (-17...
April 20, 2018: Environment International
https://www.readbyqxmd.com/read/29682163/increased-f-2-isoprostane-levels-in-semen-and-immunolocalization-of-the-8-iso-prostaglandin-f-2-%C3%AE-in-spermatozoa-from-infertile-patients-with-varicocele
#5
Giulia Collodel, Elena Moretti, Mariangela Longini, Nicola Antonio Pascarelli, Cinzia Signorini
Polyunsaturated fatty acid damages lead to alterations in sperm function. This study aimed to investigate the involvement of F2 -isoprostanes (F2 -IsoPs), oxidized lipid products from arachidonic acid, in sperm quality impairment. For this purpose, F2 -IsoP levels in semen and F2 -IsoP localization in spermatozoa were explored in infertile subjects affected by idiopathic infertility or varicocele, as well as in fertile men. As compared to fertile men, in the idiopathic infertility and varicocele groups, sperm concentration, motility, morphology, viability, and fertility index were significantly lower and the mean scores concerning sperm apoptosis, necrosis, and immaturity were significantly higher...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29678467/effect-of-achillea-millefolium-loaded-nanophytosome-in-the-post-thawing-sperm-quality-and-oxidative-status-of-rooster-semen
#6
Davood Najafi, Ramezan Ali Taheri, Abouzar Najafi, Abbas Abbas Rouhollahi, Manuel Alvarez-Rodriguez
The aim of this study was to compare the effectiveness of antioxidants including Achillea millefolium extract (AmE) (n0t1.5: 1.5, n0t3: 3 and n0t4.5: 4.5 mg/L) and AmE loaded in nano phytosome (n1t1.5: 1.5, n1t3: 3 and n1t4.5: 4.5 mg/L) in the freezing of Ross 308 rooster semen. Sperm motility (CASA), membrane integrity (HOS test), viability, total abnormality and enzymatic parameters (SOD, CAT and GPx) were assessed after thawing. AmE-loaded nano phytosome at a concentration of 3 mg/l resulted in significantly (P < 0...
April 17, 2018: Cryobiology
https://www.readbyqxmd.com/read/29676290/increased-expression-of-pelp1-in-human-sperm-is-correlated-with-decreased-semen-quality
#7
Izabela Skibinska, Miroslaw Andrusiewicz, Michal Soin, Magdalena Jendraszak, Paulina Urbaniak, Piotr Jedrzejczak, Malgorzata Kotwicka
Proline-, glutamic acid-, and leucine-rich protein 1 (PELP1) is a scaffolding protein involved in both genomic and nongenomic estrogen signal transduction pathways. To date, the role of PELP1 protein has yet to be characterized in human sperm and has not been associated with sperm parameters. To confirm the presence of PELP1 in human sperm, fresh semen samples were obtained from 178 donors. The study was designed to establish both mRNA and protein presence, and protein cellular localization. Additionally, the number of PELP1-positive spermatozoa was analyzed in men with normal and abnormal semen parameters...
April 20, 2018: Asian Journal of Andrology
https://www.readbyqxmd.com/read/29673661/vitamin-c-attenuates-negative-effects-of-vitrification-on-sperm-parameters-chromatin-quality-apoptosis-and-acrosome-reaction-in-neat-and-prepared-normozoospermic-samples
#8
Esmat Mangoli, Ali Reza Talebi, Morteza Anvari, Fatemeh Taheri, Mahboobeh Vatanparast, Tahereh Rahiminia, Akram Hosseini
OBJECTIVE: Aim of this study was to evaluate the effects of vitamin C on sperm parameters, sperm chromatin quality and apoptosis resulted of vitrification in neat semen and prepared spermatozoa of normozoospermic samples. MATERIAL AND METHODS: Forty semen samples from normozoospermic men were included in this prospective study. Each sample was divided into five groups. Group I: control or fresh semen, group II: semen prepared by swim-up method and then vitrified, group III: neat semen was vitrified, group IV: vitamin C (600 μM) was added to prepared spermatozoa and then vitrified and group V: vitamin C (600 μM) was added to neat semen and then vitrified...
April 2018: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29672007/how-general-semen-quality-influences-the-blastocyst-formation-rate-analysis-of-4205-ivf-cycles
#9
Mariana M Piccolomini, Tatiana Cs Bonetti, Eduardo La Motta, Paulo C Serafini, Jose R Alegretti
OBJECTIVE: To select embryos with higher implantation potential, the extended culture has been the most frequently applied strategy worldwide, and consequently leads to higher live birth rates per transfer. Sperm quality is a determining feature, and it may influence the outcomes of IVF from fertilization to embryo development. Therefore, we hypothesize that blastocyst formation may also be impaired by general semen quality. METHODS: We analyzed 4205 IVF cycles...
April 19, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29670898/effects-of-withania-somnifera-on-reproductive-system-a-systematic-review-of-the-available-evidence
#10
REVIEW
Ramin Nasimi Doost Azgomi, Afshar Zomorrodi, Hossein Nazemyieh, Seyed Mohammad Bagher Fazljou, Homayoun Sadeghi Bazargani, Fatemeh Nejatbakhsh, Arezoo Moini Jazani, Yadollah Ahmadi AsrBadr
Introduction: Withania somnifera (WS) also known as ashwagandha is a well-known medicinal plant used in traditional medicine in many countries for infertility treatment. The present study was aimed at systemically reviewing therapeutic effects of WS on the reproductive system. Methods: This systematic review study was designed in 2016. Required data were obtained from PubMed, Scopus, Google Scholar, Cochrane Library, Science Direct, Web of Knowledge, Web of Science, and manual search of articles, grey literature, reference checking, and expert contact...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29659832/average-sperm-count-remains-unchanged-despite-reduction-in-maternal-smoking-results-from-a-large-cross-sectional-study-with-annual-investigations-over-21-years
#11
L Priskorn, L Nordkap, A K Bang, M Krause, S A Holmboe, D L Egeberg Palme, S B Winge, N Mørup, E Carlsen, U N Joensen, M Blomberg Jensen, K M Main, A Juul, N E Skakkebaek, T K Jensen, N Jørgensen
STUDY QUESTION: How are temporal trends in lifestyle factors, including exposure to maternal smoking in utero, associated to semen quality in young men from the general population? SUMMARY ANSWER: Exposure to maternal smoking was associated with lower sperm counts but no overall increase in sperm counts was observed during the study period despite a decrease in this exposure. WHAT IS KNOWN ALREADY: Meta-analyses suggest a continuous decline in semen quality but few studies have investigated temporal trends in unselected populations recruited and analysed with the same protocol over a long period and none have studied simultaneous trends in lifestyle factors...
April 11, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29655722/a-new-strategy-for-statistical-analysis-based-fingerprint-establishment-application-to-quality-assessment-of-semen-sojae-praeparatum
#12
Hui Guo, Zhen Zhang, Yuan Yao, Jialin Liu, Ruirui Chang, Zhao Liu, Hongyuan Hao, Taohong Huang, Jun Wen, Tingting Zhou
Semen sojae praeparatum with homology of medicine and food is a famous traditional Chinese medicine. A simple and effective quality fingerprint analysis, coupled with chemometrics methods, was developed for quality assessment of Semen sojae praeparatum. First, similarity analysis (SA) and hierarchical clusting analysis (HCA) were applied to select the qualitative markers, which obviously influence the quality of Semen sojae praeparatum. 21 chemicals were selected and characterized by high resolution ion trap/time-of-flight mass spectrometry (LC-IT-TOF-MS)...
August 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29655402/determining-the-relationship-between-bull-sperm-kinematic-subpopulations-and-fluorescence-groups-using-an-integrated-sperm-quality-analysis-technique
#13
J L Yániz, I Palacín, K S Caycho, C Soler, M A Silvestre, P Santolaria
The aim of the present study was to determine whether there is an association between the kinematic sperm subpopulations and fluorescent groups in bulls using a new fluorescent staining method that allows classification of spermatozoa into groups depending on their acrosomal and membrane integrity, as well as functional status, without inhibiting sperm motility. Cryopreserved semen samples from 10 Holstein bulls were used in the study. A multiparametric analysis of results obtained by the ISAS 3Fun kit (Proiser) was performed...
April 16, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29653389/how-does-the-microbial-load-affect-the-quality-of-equine-cool-stored-semen
#14
E Varela, J Rey, E Plaza, P Muñoz de Propios, J M Ortiz-Rodríguez, M Álvarez, L Anel-López, L Anel, P De Paz, M C Gil, J M Morrell, C Ortega-Ferrusola
Contaminating bacteria present in stallion ejaculates may compromise sperm quality during storage. Different procedures have been used to reduce the load of microorganisms in semen and avoid bacterial growth during storage. The aims of this study were: 1) to evaluate different techniques to eliminate bacteria in semen 2) to study the relationship between total microflora load (TML) and ROS production; and 3) to determine if TML affects the functionality of cool-stored sperm. Ejaculates from 11 stallions were split and processed in 3 ways: A...
March 26, 2018: Theriogenology
https://www.readbyqxmd.com/read/29651938/semen-proteomics-reveals-the-impact-of-enterococcus-faecalis-on-male-fertility
#15
Giuseppe Grande, Federica Vincenzoni, Francesca Mancini, Silvia Baroni, Giovanni Luca, Riccardo Calafiore, Riccardo Marana, Massimo Castagnola, Alfredo Pontecorvi, Domenico Milardi
Infectious etiologies contribute to 15% of male factor infertility. Enterococcus faecalis is commonly identified in semen colture of infertile men and it is associated with significantly poorer semen quality. Aim of this study was to identify new seminal biomarkers for the male tract infection by E. faecalis, using proteomic profiling, in order to understand the effect of E. faecalis on the physiopathology of male reproduction. We included in the study ten patients seeking medical care for primary infertility with prostate-vescicular-epidydimitis and with microbiological analysis on semen and/or prostatic secretions positive for Enterococcus faecalis...
April 12, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29651158/effect-of-dietary-probiotics-on-the-semen-traits-and-antioxidative-activity-of-male-broiler-breeders
#16
Takio Inatomi, Konosuke Otomaru
This study aimed to investigate the effect of probiotics on the intestinal morphology, intestinal microflora, oxidative activity (biological antioxidant potential), and semen quality of male broiler breeders. For this, 180 Cobb male broiler breeders (60 weeks of age) were randomly distributed into two groups. The control group was fed a basal diet, and the probiotics group was fed basal diet supplemented with probiotics for 6 weeks. Probiotics containing Bacillus amyloliquefaciens TOA5001 improved the above mentioned characteristics of the male broiler breeders...
April 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29649854/effect-of-superoxide-dismutase-catalase-and-glutathione-peroxidase-supplementation-in-the-extender-on-chilled-semen-of-fertile-and-hypofertile-dogs
#17
Chiara Del Prete, Francesca Ciani, Simona Tafuri, Maria Pia Pasolini, Giovanni Della Valle, Veronica Palumbo, Lucia Abbondante, Antonio Calamo, Vincenza Barbato, Roberto Gualtieri, Riccardo Talevi, Natascia Cocchia
This study investigated the correlation between oxidative stress status and main canine sperm parameters and the effect of addition of Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione Peroxidase (GPx) combination in egg yolk tris-citrate glucose (EYT-G) extender on semen during 10 days of storage at 4° C. Ten Boxer dogs were included in this study and divided in two groups, fertile (F) and hypofertile (H), depending on pregnancy and live birth rates in the previous year. The semen evaluation was performed at the day of collection (D0) and after 5 (D5) and 10 (D10) days of cooled storage at 5° C...
April 12, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29644166/electroejaculation-combined-with-assisted-reproductive-technology-in-psychogenic-anejaculation-patients-refractory-to-penile-vibratory-stimulation
#18
Xianghu Meng, Longchang Fan, Tao Wang, Shaogang Wang, Zengjun Wang, Jihong Liu
Background: Psychogenic anejaculation (PAE) is a rare disease in clinic seriously affecting quality of life, especially fertility of patients. However, there is not an effective treatment for these patients. The aim of the study was to evaluate the results of treatment with electroejaculation (EEJ), intrauterine insemination (IUI), and intracytoplasmic sperm injection (ICSI) in patients with PAE who were refractory to penile vibratory stimulation (PVS). Methods: Twenty PVS-refractory patients with PAE were included in the retrospective study...
March 2018: Translational Andrology and Urology
https://www.readbyqxmd.com/read/29629734/changes-of-sperm-parameters-along-time-among-groups-of-different-qualities
#19
Shimi Barda, Tomer Bar-Noy, Amnon Botchan, Ofer Lehavi, Haim Yavetz, Ron Hauser
BACKGROUND: Male infertility is solely responsible for approximately 20% of all infertility in couples. Various factors have been proposed as having a negative effect on sperm quality; however, the reasons for the global decline in sperm parameters during the last few decades are still controversial. OBJECTIVES: To investigate the fluctuations of semen parameters (sperm concentration, motility, and morphology) in three sperm quality groups and to examine the trends of those parameters in the same men over time...
April 2018: Israel Medical Association Journal: IMAJ
https://www.readbyqxmd.com/read/29628206/odorant-receptor-might-be-related-to-sperm-dna-integrity-in-apis-cerana-cerana
#20
Lina Guo, Huiting Zhao, Bing Xu, Yusuo Jiang
Olfactory receptors (ORs) are important for insects to recognize and discriminate odorants in the environment and are mainly expressed in olfactory and gustatory organs. Little is known about the potential OR functions in non-olfactory tissues. In the present study, we evaluated the possibility of odorant receptors AcerOr1 and AcerOr2 (AcerOr2 is orthologous to the co-receptor) mediating sperm DNA integrity, and the relationship between sperm DNA integrity and semen parameters in Apis cerana cerana. Based on previous findings in mammals, we speculated that the Ca2+ /calmodulin (CaM)/CaM-dependent protein kinase II (CaMKII) signaling pathway might be involved in the regulation of sperm motility in A...
March 31, 2018: Animal Reproduction Science
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