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https://www.readbyqxmd.com/read/29040510/genomic-fragmentation-and-extrachromosomal-telomeric-repeats-impact-assessment-of-telomere-length-in-human-spermatozoa-quantitative-experiments-and-systematic-review
#1
P Kurjanowicz, S Moskovtsev, C Librach
STUDY QUESTION: Can differences in DNA isolation alter assessment of sperm telomere length (spTL) and do they account for conflicting results in the literature on spTL and male fertility? SUMMARY ANSWER: DNA isolation methods preferentially include or exclude short, extrachromosomal (EC) telomere-specific sequences that alter spTL measurements, and are responsible for a proportion of the disparity observed between investigations. WHAT IS KNOWN ALREADY: The relationship between spTL and male fertility has become an active area of research...
September 29, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29031946/spermatozoa-dna-methylation-patterns-differ-due-to-peripubertal-age-in-bulls
#2
Simon Lambert, Patrick Blondin, Christian Vigneault, Rémi Labrecque, Isabelle Dufort, Marc-André Sirard
In the dairy industry, using semen as soon as the bull is mature enough to produce it is advantageous for breeding purposes. Mammalian spermatogenesis is a hormone-dependent developmental program in which a complex cascade of events must take place to ensure that germ cells reach the proper stage of development at the proper time. Conventional indicators of semen quality such as sperm cell motility and viability usually improve as bulls mature, meeting quality criteria satisfactorily at around 16 months. Using semen before that age may affect embryo viability, but other changes occurring during the peripubertal period should be considered...
October 6, 2017: Theriogenology
https://www.readbyqxmd.com/read/29031884/human-sperm-cryopreservation-in-cancer-patients-links-with-deprivation-and-mortality
#3
Chey G Dearing, Channa N Jayasena, Kevin Lindsay
Evidence is mounting for a relationship between human semen quality and environmental/lifestyle/socioeconomic factors including long term health outcomes such as mortality. The relationship between pre-freeze and post-thaw semen quality in cancer patients and these factors are unknown. Frozen semen from 217 cancer patients was thawed and analysed using a validated CASA method. Post-thaw quality was matched and compared with WHO semen analysis performed prior to storage. The English Indices of Deprivation 2010 were matched with patients and then examined for relationships with ejaculate pre-freeze and post-thaw semen quality...
October 11, 2017: Cryobiology
https://www.readbyqxmd.com/read/29025071/the-expression-characteristics-of-fam71d-and-its-association-with-sperm-motility
#4
Qian Ma, Yuchi Li, Manling Luo, Huan Guo, Shouren Lin, Jianbo Chen, Ye Du, Zhimao Jiang, Yaoting Gui
STUDY QUESTION: What are the features of FAM71D (Family with sequence similarity 71, member D) expression and is there an association between FAM71D expression and sperm motility? SUMMARY ANSWER: FAM71D, a novel protein exclusively expressed in the testis, is located in sperm flagella and is functionally involved in sperm motility. WHAT IS KNOWN ALREADY: Some testis-specific proteins have been reported as potential diagnostic biomarkers to evaluate the spermatogenesis process and sperm quality...
September 12, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29024969/preconception-urinary-phthalate-concentrations-and-sperm-dna-methylation-profiles-among-men-undergoing-ivf-treatment-a-cross-sectional-study
#5
Haotian Wu, Molly S Estill, Alexander Shershebnev, Alexander Suvorov, Stephen A Krawetz, Brian W Whitcomb, Holly Dinnie, Tayyab Rahil, Cynthia K Sites, J Richard Pilsner
STUDY QUESTION: Are preconception phthalate and phthalate replacements associated with sperm differentially methylated regions (DMRs) among men undergoing IVF? SUMMARY ANSWER: Ten phthalate metabolites were associated with 131 sperm DMRs that were enriched in genes related to growth and development, cell movement and cytoskeleton structure. WHAT IS KNOWN ALREADY: Several phthalate compounds and their metabolites are known endocrine disrupting compounds and are pervasive environmental contaminants...
September 12, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29024507/dietary-patterns-and-semen-quality-a-systematic-review-and-meta-analysis-of-observational-studies
#6
REVIEW
A Arab, N Rafie, M Mansourian, M Miraghajani, H Hajianfar
A number of studies have examined the association between dietary patterns and semen quality, but the findings have been inconclusive. Herein, we conducted a systematic review and meta-analysis of observational studies to assess the association between dietary patterns and semen quality. PubMed, Cochrane library, Science direct, Scopus, Google Scholar, and ISI web of science databases were searched up to August 2016 for observational studies assessing the association between common dietary patterns and sperm quality markers...
October 12, 2017: Andrology
https://www.readbyqxmd.com/read/29022649/the-quality-of-semen-among-a-sample-of-young-healthy-men-from-lower-silesia-androls
#7
Marek Mędraś, Felicja Lwow, Paweł Jóźków, Leszek Szmigiero, Aleksandra Zagrodna, Ewa Zagocka, Małgorzata Słowińska-Lisowska
INTRODUCTION: Contrary to other parts of the continent, little information is available regarding semen quality among subjects from central and eastern Europe. MATERIAL AND METHODS: We evaluated semen profiles among a sample of men from an industrialised region of Poland. We directly invited 5000 healthy inhabitants of the region (aged 18-35 years; with unchecked fecundity) to participate in the study. Among the 500 who were eligible and willing to participate, we acquired detailed information and semen and blood samples from 177 subjects...
October 12, 2017: Endokrynologia Polska
https://www.readbyqxmd.com/read/29020465/donor-sperm-insemination-after-failed-intra-couple-intracytoplasmic-sperm-injection
#8
Sylviane Hennebicq, Oxana Blagosklonov, Florence Eustache, Aline Papaxanthos, Veronique Drouineaud, Catherine Guillemain, Sophie Mirallie, Beatrice Delepine, Nathalie Rives, Isabelle Berthaut, Cynthia Frapsauce, Jean-François Guérin, Mehdi Benchaib
The purpose of the present multicenter study was to investigate whether an artificial insemination with donor sperm (AID) procedure after intra-couple intracytoplasmic sperm injection (ICSI) failure offers a significant chance of pregnancy and to identify prognostic factors for pregnancy after an AID procedure. An eleven-year retrospective multicenter study was conducted among 13 Centre d'Etude et de Conservation des Oeufs et du Sperme (CECOS) centers. A total of 319 couples having undergone an AID procedure after intra-conjugal ICSI failure were included in this study; a total of 1,159 AID and 1,011 intra-conjugal ICSI cycles were performed...
October 11, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29016685/lactobacillus-rhamnosus-pb01-dsm-14870-supplementation-affects-markers-of-sperm-kinematic-parameters-in-a-diet-induced-obesity-mice-model
#9
Fereshteh Dardmeh, Hiva Alipour, Parisa Gazerani, Gerhard van der Horst, Erik Brandsborg, Hans Ingolf Nielsen
Probiotics have been proposed as alternatives to pharmacological products in several medical conditions including the modulation of obesity, which is frequently associated with poor semen quality. However, effects of probiotics on male fertility have been less investigated. This study assessed the effect of Lactobacillus rhamnosus PB01 (DSM-14870) on sperm kinematic parameters in Normal-weight (NW) and diet-induced obese (DIO) models. NW and DIO C57BL/6NTac mice were divided into two subgroups with or without a single daily dose (1x109CFU) of L...
2017: PloS One
https://www.readbyqxmd.com/read/28992366/possible-involvement-of-the-glucocorticoid-receptor-nr3c1-and-selected-nr3c1-gene-variants-in-regulation-of-human-testicular-function
#10
L Nordkap, K Almstrup, J E Nielsen, A K Bang, L Priskorn, M Krause, S A Holmboe, S B Winge, D L Egeberg Palme, N Mørup, J H Petersen, A Juul, N E Skakkebaek, E Rajpert-De Meyts, N Jørgensen
Perceived stress has been associated with decreased semen quality but the mechanisms have not been elucidated. It is not known whether cortisol, the major stress hormone in humans, can act directly via receptors in the testis, and whether variants in the gene encoding the glucocorticoid receptor (NR3C1) can possibly modulate the effect. To address these questions, we investigated the expression of the glucocorticoid receptor in human testicular tissue, including adult and fetal samples (n = 20) by immunohistochemical staining, and in silico analysis of publicly available datasets...
October 9, 2017: Andrology
https://www.readbyqxmd.com/read/28991532/a-new-approach-for-identification-of-medicinal-almonds-by-fourier-transform-infrared-spectroscopy-and-systematic-clustering-of-characteristic-peaks
#11
Chun-Song Cheng, Can-Jian Wang, Jie Liang, Chi-Chou Lao, Hua Zhou, Zhi-Feng Zhang
Medicinal almonds have been used for over 2 000 years and its clinical efficacy includes relieving cough and asthma. The domestic market in China is flooded with different kinds of dried almonds, such as bitter almond (Armeniacae Semen Amarum, AAS), sweet almond (Armeniacae Semen Dulce, ADS), salted almond (Armeniacae Semen Salsa, ASS), and their sulfur-fumigating products (Armeniacae Semen Sulphur Fumabat, ASFS). Wide varieties of almonds may lead to uncertain efficacy, aberrant quality, and even increased safety risk...
September 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28988559/ram-semen-deterioration-by-short-term-exposure-to-high-altitude-is-prevented-by-improvement-of-antioxidant-status
#12
E Cofré, O A Peralta, A Raggi, M De Los Reyes, F Sales, A González-Bulnes, V H Parraguez
Ovine reproduction efficiency in herds at high altitude (ha) is lower than that at low altitude (la). In ewes, ha effects are due to hypoxia and oxidative stress. Our aim was to establish the effect of antioxidant vitamin supplementation on semen traits and antioxidant status of rams exposed to short or long time ha. A total of 32 rams native to la (~500 m) were used, 16 were kept at la and the other 16 were brought to ha (~3600 m), where they were placed in the same flock as the ha native rams (n=16). Half of the animals in each group were supplemented daily with vitamins C 600 mg and E 450 IU per os, during the entire experimental period, starting the 4th day after animal's arrival at ha (day 0)...
October 9, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28986135/impact-of-supplementation-of-semen-extender-with-antioxidants-on-the-quality-of-chilled-or-cryopreserved-arabian-stallion-spermatozoa
#13
sAbdelRaouf M Ghallab, Abdallah M Shahat, Aya M Fadl, Mohamed M Ayoub, Adel R Moawad
The aim of the present study was to evaluate the effects of supplementation of semen extender with various non-enzymatic antioxidants on the quality of cooled or cryopreserved Arabian stallion spermatozoa. Semen collected from four pure Arabian stallions was centrifuged at 600g for 15 min. Spermatozoa were then diluted in INRA-82 extender supplemented with bovine serum albumin (BSA; 0, 10, 15 and 20 mg/mL) or trehalose (0, 75, 100 and 150 mM) or zinc sulphate (0, 100, 150 and 200 μM). The diluted semen was then either cooled at 5 °C or cryopreserved in 0...
October 4, 2017: Cryobiology
https://www.readbyqxmd.com/read/28985041/influence-of-the-abstinence-period-on-human-sperm-quality-analysis-of-2-458-semen-samples
#14
Vanessa A Comar, Claudia G Petersen, Ana L Mauri, Mariana Mattila, Laura D Vagnini, Adriana Renzi, Bruna Petersen, Andreia Nicoletti, Felipe Dieamant, Joao Batista A Oliveira, Ricardo L R Baruffi, José G Franco
OBJECTIVE: The aim of this study was to evaluate the influence of different periods of abstinence on conventional semen parameters as well as functional parameters in human semen, including mitochondrial function, chromatin packing and sperm DNA fragmentation. METHODS: We recruited a cohort of 2,458 men undergoing infertility investigation. Semen analyses were performed according to WHO guidelines/morphology-motile sperm organelle morphology examination/MSOME. For DNA integrity analysis, the percentages of DNA fragmentation (TUNEL), abnormal chromatin packaging/underprotamination (chromomycin A3/CMA3), abnormal mitochondrial membrane potential (MMP/MitoTracker Green), and apoptosis (annexin-V) were recorded...
October 6, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28981651/the-diagnosis-of-male-infertility-an-analysis-of-the-evidence-to-support-the-development-of-global-who-guidance-challenges-and-future-research-opportunities
#15
Christopher L R Barratt, Lars Björndahl, Christopher J De Jonge, Dolores J Lamb, Francisco Osorio Martini, Robert McLachlan, Robert D Oates, Sheryl van der Poel, Bianca St John, Mark Sigman, Rebecca Sokol, Herman Tournaye
BACKGROUND: Herein, we describe the consensus guideline methodology, summarize the evidence-based recommendations we provided to the World Health Organization (WHO) for their consideration in the development of global guidance and present a narrative review of the diagnosis of male infertility as related to the eight prioritized (problem or population (P), intervention (I), comparison (C) and outcome(s) (O) (PICO)) questions. Additionally, we discuss the challenges and research gaps identified during the synthesis of this evidence...
July 19, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/28974479/prenatal-and-postnatal-pcb-153-and-p-p-dde-exposures-and-behavior-scores-at-5-9-years-of-age-among-children-in-greenland-and-ukraine
#16
Aske Hess Rosenquist, Birgit Bjerre Høyer, Jordi Julvez, Jordi Sunyer, Henning Sloth Pedersen, Virissa Lenters, Bo A G Jönsson, Jens Peter Bonde, Gunnar Toft
BACKGROUND: Studies have reported some evidence of adverse effects of organochlorine exposures on child development, but the results have been inconsistent, and few studies have evaluated associations with child behavior. OBJECTIVE: We investigated the association between prenatal and early-life exposures to 2,2',4,4',5,5'-hexachlorobiphenyl (PCB-153) and 1,1-dichloro-2,2-bis(p-chlorophenyl)-ethylene (p,p'-DDE) and behaviors in children between 5 and 9 y of age...
October 3, 2017: Environmental Health Perspectives
https://www.readbyqxmd.com/read/28969455/the-impact-of-cigarette-smoking-on-protamines-1-and-2-transcripts-in-human-spermatozoa
#17
Mohammed Hamad, Nyaz Shelko, Mathias Montenarh, Mohammed Eid Hammadeh
This study was conducted to evaluate the relation between cigarette smoking, semen quality and ratios of protamine mRNAs in smokers and non-smokers. Spermatozoa from 123 men and 64 smokers and 59 non-smokers whose female partners attended an assisted reproduction and andrology laboratory were evaluated. Protamine mRNA was extracted from purified sperm, reverse-transcribed and subjected to real-time quantitative PCR using specific primer pairs for protamine 1 (PRM1) and protamine 2 (PRM2). The main outcomes showed that PRM1 mRNA levels in smokers were significantly lower (p = 0...
October 2, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28969406/effect-of-superoxide-dismutase-supplementation-on-sperm-dna-fragmentation
#18
Luciano Negri, Renzo Benaglia, Emanuela Monti, Emanuela Morenghi, Alessandro Pizzocaro, Paolo E Levi Setti
BACKGROUND: antioxidants supplementation improves sperm quality, but few trials have analyzed the effects on sperm DNA fragmentation (SDF). This study compares the effectiveness of SOD-based antioxidant supplementation plus hydroxytyrosol and carnosol in reducing SDF with other antioxidants without SOD, hydroxytyrosol, and carnosol. MATERIALS AND METHODS: men with high SDF at baseline were selected in our clinical database. The patients taken into account had a 2-month control...
October 3, 2017: Archivio Italiano di Urologia, Andrologia
https://www.readbyqxmd.com/read/28967061/effects-of-electromagnetic-waves-emitted-from-3g-wi-fi-modems-on-human-semen-analysis
#19
Koosha Kamali, Mohammadmehdi Atarod, Saeedeh Sarhadi, Javad Nikbakht, Maryam Emami, Robab Maghsoudi, Hormoz Salimi, Bita Fallahpour, Negar Kamali, Abdolreza Momtazan, Mojtaba Ameli
OBJECTIVE: The purpose of this study was to evaluate the effects of 3G+wifi modems on human sperm quality.A total of 40 semen specimens were gathered between March and September 2015, from healthy adult men. METHODS: The sperm samples were divided into two groups - 3G+wi-fi exposed and unexposed groups. In the unexposed group, the specimens were shielded by aluminum foil in three layers and put into an incubator at a temperature of 37°C for 50 minutes. The exposed group was positioned in another room in an incubator at a temperature of 37°C for 50 minutes...
September 14, 2017: Urologia
https://www.readbyqxmd.com/read/28965574/effect-of-selenium-on-testicular-damage-induced-by-varicocele-in-adult-male-wistar-rats
#20
Leila Taghizadeh, Akram Eidi, Pejman Mortazavi, Ali Haeri Rohani
PROJECT: Varicocele is an abnormal tortuosity and distension of the veins of the pampiniform plexus in the spermatic cord. It is the most common surgically correctable cause of male infertility. Several studies have revealed the effects of increased oxidative stress on serum, semen, and testicular tissues in patients with varicocele or in animal models. The aim of this study was to investigate the effects of sodium selenite on testicular damage induced by experimental left varicocele in male Wistar rats...
December 2017: Journal of Trace Elements in Medicine and Biology
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