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Semen quality

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https://www.readbyqxmd.com/read/28078721/use-of-the-fluorescent-dye-tetramethylrhodamine-methyl-ester-perchlorate-for-mitochondrial-membrane-potential-assessment-in-human-spermatozoa
#1
P Uribe, J V Villegas, R Boguen, F Treulen, R Sánchez, P Mallmann, V Isachenko, G Rahimi, E Isachenko
Mitochondrial membrane potential (ΔΨm) is an indicator of sperm quality and its evaluation complements the standard semen analysis. The fluorescent dye JC-1 has been widely used to assess sperm ΔΨm; however, some problems have been detected under certain experimental conditions. Another fluorescent compound, tetramethylrhodamine methyl ester perchlorate (TMRM), has been used in somatic cells and bovine spermatozoa but not in human spermatozoa. TMRM accumulates in hyperpolarised mitochondria and the fluorescence intensity of this compound correlates with ΔΨm...
January 11, 2017: Andrologia
https://www.readbyqxmd.com/read/28077271/occupational-exposure-to-pesticides-reproductive-hormone-levels-and-sperm-quality-in-young-brazilian-men
#2
Cleber Cremonese, Camila Piccoli, Fabio Pasqualotto, Ruth Clapauch, Rosalina Jorge Koifman, Sergio Koifman, Carmen Freire
The association of occupational exposure to current-use pesticides with reproductive hormones, semen quality, and genital measures was investigated among young men in the South of Brazil. A cross-sectional study was conducted in 99 rural and 36 urban men aged 18-23 years. Information on pesticide use was obtained through questionnaire. Serum and semen samples were analyzed for sex hormones and sperm parameters, respectively, and measurement of anogenital distance (AGD) and testis volume (TV) were performed...
January 7, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28076980/status-of-sperm-born-oocyte-activating-factors-pawp-plc%C3%AE-and-sperm-chromatin-in-uncapacitated-capacitated-and-acrosome-reacted-conditions
#3
Marziyeh Tavalaee, Kazem Parivar, Abdol-Hossein Shahverdi, Kamran Ghaedi, Mohammad Hossein Nasr-Esfahani
The aim of this study was to investigate the value of sperm factors PAWP and PLCζ involved in oocyte activation and sperm chromatin status, as indicators of sperm quality during uncapacitated, capacitated and acrosome-reacted conditions. Semen samples collected from 10 normozoospermic men and sperm factors PAWP, PLCζ, protamine deficiency and DNA fragmentation evaluated in fresh samples, processed sample by DGC (density gradient centrifugation), capacitated and acrosome reacted sperm, using immunofluorescence and flow cytometry...
January 12, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28070708/delayed-treatment-of-undescended-testes-may-promote-hypogonadism-and-infertility
#4
Julia Rohayem, Alessandra Luberto, Eberhard Nieschlag, Michael Zitzmann, Sabine Kliesch
CONTEXT: Undescended testes at birth may be caused by testosterone deficiency during fetal development. It is unclear whether the process of failed descent contributes to permanent endocrine impairment. OBJECTIVES: To evaluate the impact of age at treatment of undescended testes on endocrine and spermatogenic testicular function in middle-aged men. PATIENTS AND METHODS: Reproductive hormone and semen data of 357 men with previously undescended testes were evaluated with respect to age at correction of testicular position and compared to those of 709 controls with eutopic testes at birth and normozoospermia...
January 9, 2017: Endocrine
https://www.readbyqxmd.com/read/28066955/dose-rates-of-antimicrobial-substances-in-boar-semen-preservation-time-to-establish-new-protocols
#5
M Schulze, M Grobbel, A Riesenbeck, S Brüning, J Schaefer, M Jung, R Grossfeld
To achieve a standardized number of spermatozoa in the final AI dose, varying amounts of extender fluid with a fixed concentration of antimicrobial substances are currently added to boar ejaculates. This practice ignores the different degrees of dilution of the antimicrobials in the end product. In calculating the final concentration of gentamicin in AI doses from 27,538 processed boar ejaculates, we demonstrated varying gentamicin concentrations in the resultant extended boar semen samples. The median concentration was 220...
January 9, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28066832/antioxidant-supplements-and-semen-parameters-an-evidence-based-review
#6
REVIEW
Sedigheh Ahmadi, Reihane Bashiri, Akram Ghadiri-Anari, Azadeh Nadjarzadeh
Many studies have focused on male infertility. There is limited evidence about the influence of nutrition on quality of semen. Approximately, 30-80% of infertility cases are caused by oxidative stress and decreased level of seminal total antioxidant capacity. This study was aimed to review the effects of oral antioxidant supplements on improving major semen parameters such as sperm concentration, motility, morphology, DNA damage, and fertility rate. Data were extracted from PubMed and Google scholar database by using the terms "antioxidant", "multivitamin", "carnitine", "CoQ10", "vitamin C", "vitamin E", "zinc", "folic acid", "N-acetyl cysteine" and "selenium" combined with "male infertility", "semen", and "sperm" to generate a set of relevant citations...
December 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/28058745/lipid-and-protein-oxidation-levels-in-spermatozoa-and-seminal-plasma-of-asian-elephants-elephas-maximus-and-their-relationship-with-semen-parameters
#7
S Satitmanwiwat, K Promthep, K Buranaamnuay, S Mahasawangkul, K Saikhun
Peroxidation damage to spermatozoa and seminal plasma has an important role in sperm quality. Thus, the objective of this study was to determine the levels of lipid and protein oxidation in spermatozoa and seminal plasma of Asian elephants (Elephas maximus) with varying percentage of progressive motility. Lipid and protein oxidation was measured by the thiobarbituric acid-reactive species (TBARS) assay and the 2, 4-dinitrophenylhydrazine (DNPH) carbonyl groups assay, respectively. Fresh semen samples were collected from Asian elephants and classified according to the percentage of motile spermatozoa into good (>60%) and poor (≤20%) motility...
January 6, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28058738/epigallocatechin-3-gallate-egcg-and-green-tea-polyphenols-do-not-improve-stallion-semen-parameters-during-cooling-at-4%C3%A2-c
#8
D Bucci, M Spinaci, B Mislei, B Gadani, G Rizzato, C C Love, C Tamanini, G Galeati, G Mari
Stallion semen storage for artificial insemination is mainly based on liquid cooled storage. In many stallions this technique maintains sperm quality for an extended period of time (24-72 hr) at 7°C. While this technique is commonly used in the horse industry, there can be a decline in fertility in some stallions, due to an inability of their sperm to tolerate the cool storage process. The aim of the present work was to evaluate the effect of two natural antioxidants (epigallocatechin-3-gallate (EGCG) at 20, 60 and 120 μm and green tea polyphenols, and p at ...
January 6, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28052756/small-rna-sequencing-of-cryopreserved-semen-from-single-bull-revealed-altered-mirnas-and-pirnas-expression-between-high-and-low-motile-sperm-populations
#9
E Capra, F Turri, B Lazzari, P Cremonesi, T M Gliozzi, I Fojadelli, A Stella, F Pizzi
BACKGROUND: Small RNAs present in bovine ejaculate can be linked to sperm abnormalities and fertility disorders. At present, quality parameters routinely used in semen evaluation are not fully reliable to predict bull fertility. In order to provide additional quality measurements for cryopreserved semen used for breeding, a method based on deep sequencing of sperm microRNA (miRNA) and Piwi-interacting RNA (piRNA) from individual bulls was developed. To validate our method, two populations of spermatozoa isolated from high and low motile fractions separated by Percoll were sequenced, and their small RNAs content characterized...
January 4, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28050956/the-effects-of-storing-and-transporting-cryopreserved-semen-samples-on-dry-ice
#10
David Til, Vera L L Amaral, Rafael A Salvador, Alfred Senn, Thais S de Paula
OBJECTIVE: This study aimed to test the effects on sperm viability of transporting cryopreserved semen samples on dry ice. METHODS: Twenty normozoospermic semen samples were cryopreserved and divided into five groups. The samples in Group 1 were immersed in liquid nitrogen throughout the experiment in cryogenic storage tanks; the cryopreserved straws in Group 2 were placed in a Styrofoam box containing dry ice and kept under these conditions for 48 hours; the samples in Group 3 were kept for 48 hours on dry ice under the same conditions as the Group 2 samples, and were then moved to a storage tank filled with liquid nitrogen; Group 4 samples were also kept for 48 hours in dry ice storage, and the Styrofoam box containing the samples was shipped by plane to assess the effects of shipping; the samples in Group 5 were shipped together with the Group 4 samples and were placed in a storage tank with liquid nitrogen after spending 48 hours stored on dry ice...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28050014/semen-quality-in-the-21-st-century
#11
REVIEW
Helena E Virtanen, Niels Jørgensen, Jorma Toppari
Although semen quality is an important determinant of fertility, defining clear thresholds for normal ranges has proven difficult. According to 'time to pregnancy' studies, fecundity starts to decline when sperm concentrations fall below 30-55 × 10(6)/ml, whereas the WHO criterion for normal values is currently 15 × 10(6)/ml. Multiple studies over the past 15 years have reported median sperm concentrations of 41-55 × 10(6)/ml in young men (mean age 18-21 years) from the general population, suggesting that many of them have suboptimal semen quality...
January 4, 2017: Nature Reviews. Urology
https://www.readbyqxmd.com/read/28048968/supplementing-rooster-sperm-with-cholesterol-loaded-cyclodextrin-improves-fertility-after-cryopreservation
#12
Napapach Chuaychu-Noo, Pachara Thananurak, Vibantita Chankitisakul, Thevin Vongpralub
Little is known about the effects of Cholesterol-Loaded Cyclodextrin (CLC) on post-thaw semen quality in chicken. The aim of the present study is to investigate the efficacy of CLC levels (0, 1, 2 and 3 mg/mL Schramm diluent) on post-thawed semen quality and fertility in two breeds of chicken Pradu Hang Dum (native chicken) and Rhode Island Red. Semen samples of each breed were pooled, divided into 4 aliquots and diluted with Schramm diluents, cooled to 5 °C when DMF was added (6% of final volume). Semen straws were subjected to cryopreservation using the liquid nitrogen vapor method...
December 31, 2016: Cryobiology
https://www.readbyqxmd.com/read/28043372/relationships-among-stallion-fertility-and-semen-traits-using-estimated-breeding-values-of-german-warmblood-stallions
#13
Maren Gottschalk, Harald Sieme, Gunilla Martinsson, Ottmar Distl
A high quality of stallion semen is of particular importance for maximum reproductive efficiency. In the present study, we estimated the relationships among estimated breeding values (EBVs) of semen traits and EBVs for the paternal component of the pregnancy rate per estrus cycle (EBV-PAT) for 100 German Warmblood stallions using correlation and general linear model analyses. The most highly correlated sperm quality trait was total number of progressively motile sperm (r = 0.36). EBV-PAT was considered in three classes with stallions 1 SD below (<80), around (80-120), and above (>120) the population mean of 100...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043366/semen-cryopreservation-and-radical-reduction-capacity-of-seminal-fluid-in-captive-african-lion-panthera-leo
#14
I Luther, U Jakop, I Lueders, A Tordiffe, C Franz, J Schiller, A Kotze, K Müller
Optimizing cryopreservation protocols for nondomestic felids contributes to the successful development of assisted reproduction techniques and genetic resource banking. In this study, we describe a simple cryopreservation procedure for African lion (Panthera leo) ejaculates, which was tested with different packaging options and different sperm numbers per dose. By applying urethral catheterization and electroejaculation, 17 ejaculates with greater than 20% motile and greater than 5% progressively motile sperm were collected...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043365/cooled-storage-of-equine-semen-does-not-induce-major-changes-in-sperm-dna-methylation
#15
Rodrigo Arruda de Oliveira, Dragos Scarlet, Natascha Ille, Christine Aurich
A decrease in fertility of equine semen during cooled-storage so far has mainly been attributed to changes in sperm membrane function. In the present study we hypothesized that cooled-storage also changes the sperm DNA methylation level. For this purpose, semen was collected from 10 fertile stallions and processed for cooled-storage at 5 °C. Two final concentrations, 50 × 10(6) and 100 × 10(6) cells/mL, were used. Semen was analyzed for total motility, progressive motility, membrane integrity, phosphatidylserine translocation (PST), mitochondrial membrane potential and chromatin condensation, immediately after processing and at 24 h-intervals until 72 h of storage...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043360/effects-of-an-extension-of-the-equilibration-period-up-to-96%C3%A2-hours-on-the-characteristics-of-cryopreserved-bull-semen
#16
A Fleisch, E Malama, U Witschi, C Leiding, M Siuda, F Janett, H Bollwein
This study was designed to investigate the effects of an equilibration period up to 96 hours and three extenders (AndroMed, OPTIXcell, and Triladyl) on the quality of cryopreserved bull semen and to evaluate, whether an extension of the equilibration time to 72 hours does affect fertility in the field. One ejaculate of 17 bulls was collected and divided into three equal aliquots and diluted, respectively, with the three extenders. Each aliquot was again divided into five parts and equilibrated for 4, 24, 48, 72, and 96 hours before freezing in an automatic freezer...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043357/do-antimicrobial-peptides-pr-39-pmap-36-and-pmap-37-have-any-effect-on-bacterial-growth-and-quality-of-liquid-stored-boar-semen
#17
Eva Bussalleu, Sílvia Sancho, Maria D Briz, Marc Yeste, Sergi Bonet
The use of antimicrobial peptides (AMP) has become one of the most promising alternatives to the use of antibiotics (Abs) in semen extender's formulation to overcome the increasing bacterial resistance to antibiotics. However, AMP may impair boar sperm quality, so that their deleterious effects might be higher than their effectiveness against bacteria. Thus, the aim of this study was to determine whether three different AMP, the proline-arginine-rich antimicrobial peptide PR-39 (PR-39), and the porcine myeloid antimicrobial peptides 36 (PMAP-36) and 37 (PMAP-37) had any effect upon boar sperm quality and bacterial growth...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043354/effect-of-sex-sorting-and-cryopreservation-on-the-post-thaw-sperm-quality-of-iberian-red-deer-spermatozoa
#18
L Anel-López, O García-Álvarez, I Parrilla, D Del Olmo, A Maroto-Morales, M R Fernandez-Santos, J A Ortiz, A J Soler, E M Martínez, J M Vazquez, J J Garde
This study investigated the effect of sex-sorting and cryopreservation on post-thaw characteristics and fertility of red deer (Cervus elaphus) sperm for the first time. Semen was collected by electroejaculation from 10 mature stags during the breeding season, and each ejaculate split into four experimental groups: Bulk sorted spermatozoa, sorted but not sexed (BSS); sorted high purity X-spermatozoa (XSS); sorted high purity Y-spermatozoa (YSS); and, control non-sorted spermatozoa (NS). Following, all samples were frozen over liquid nitrogen...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043349/effects-of-guanidinoacetic-acid-diet-supplementation-on-semen-quality-and-fertility-of-broiler-breeder-roosters
#19
Ramin Shahabi Tapeh, Mahdi Zhandi, Mojtaba Zaghari, Amir Akhlaghi
Decreased semen quality and fertility rate is a common feature in broiler breeder roosters. This decrease is associated with dysfunction of Sertoli cells and defective spermatogenesis. Guanidinoacetic acid (GAA), as a precursor of creatine, plays an important role in the proper functioning of Sertoli cells and energy metabolism in sperm. Twenty, 29-wk-old broiler breeder roosters (Ross 308) were randomly allotted to 4 treatment groups and fed diets supplemented with different levels of GAA, including 0 (GAA-0), 600 (GAA-600), 1200 (GAA-1200), and 1800 (GAA-1800) mg GAA/kg of diet for 26 successive weeks...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043348/the-value-of-trans-scrotal-ultrasonography-at-bull-breeding-soundness-evaluation-bbse-the-relationship-between-testicular-parenchymal-pixel-intensity-and-semen-quality
#20
Martin Tomlinson, Amy Jennings, Alastair Macrae, Isabelle Truyers
Bull breeding soundness evaluation (BBSE) is commonly undertaken to identify bulls that are potentially unfit for use as breeding sires. Various studies worldwide have found that approximately 20% of the bulls fail their routine prebreeding BBSE and are therefore considered subfertile. Multiple articles describe the use of testicular ultrasound as a noninvasive aid in the identification of specific testicular and epididymal lesions. Two previous studies have hypothesized a correlation between ultrasonographic testicular parenchymal pixel intensity (PI) and semen quality; however to date, no published studies have specifically examined this link...
February 2017: Theriogenology
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