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Semen quality

Masoud Hamerezaee, Somayeh F Dehghan, Farideh Golbabaei, Asad Fathi, Loghman Barzegar, Naseh Heidarnejad
Background: This study was conducted to investigate the heat stress and semen quality among male workers in a steel industry in Iran and investigate the relationship between heat stress indices and semen parameters. Methods: The study was conducted on workers exposed ( n  = 30) and unexposed ( n  = 14) to heat in a steel industry. After obtaining a brief biography of the selected employees, scrotal temperature, oral temperature, and environmental parameters were measured, and their semen samples were analyzed according to the procedure recommended by the World Health Organization...
June 2018: Safety and Health At Work
Eva Tvrdá, Francisca Arroyo, Jaime Gosálvez
AIM: The purpose of the study was to evaluate the impact of seminal plasma in human ejaculates on the sperm DNA quality and DNA longevity. METHODS: Semen samples for this study were obtained from 20 donors with a normal spermiogram. Following centrifugation, the sperm pellet was resuspended either in the seminal plasma proceeding from its respective donor, or in an equal amount of PBS, adjusting the concentration to 50 × 106 /ml. Each set of samples was incubated at 37 °C for 24 h and the sperm DNA damage was assessed using the chromatin-dispersion test following 0, 2, 6, and 24 h of incubation...
June 20, 2018: International Urology and Nephrology
Sadia Naz, Muhammad Umair, Sajid Iqbal
Our objectives were to ascertain the comparison of Tris egg yolk-based, Triladyl® and Optixell® extender on postthaw quality, CASA parameters and in vivo fertility of Nili Ravi buffalo (Bubalus bubalis) bull spermatozoa. Semen samples (n = 35) from five bulls were diluted in Tris egg yolk-based, Triladyl® , Optixell® extender and frozen in 0.50 ml French straws. Postthaw sperm CASA motility (%) was higher (p < 0.05) in Optixell extender as compared to Triladyl and Tris egg yolk-based extender. Although sperm progressive motility (%), morphology (%), average path velocity (μm/s), straight line velocity (μm/s), curvilinear velocity (μm/s), amplitude of lateral head displacement (μm), beat cross-frequency (Hz), straightness (%), length of curvilinear path (μm), length of average path (μm), intact plasma and acrosome membrane (%), and DNA integrity (%) were higher (p < 0...
June 19, 2018: Andrologia
Anne Grosen, Jacob Nersting, Mona Bungum, Lisbet Ambrosius Christensen, Kjeld Schmiegelow, Marcello Spanò, Mette Julsgaard, Eugenia Cordelli, Giorgio Leter, Peter Braad Larsen, Christian Lodberg Hvas, Jens Kelsen
Background & Aims: Sperm DNA integrity, concentration and motility are suspected to be altered by thiopurines (azathioprine (AZA) and 6-mercaptopurine (6-MP)). We investigated the impact of thiopurines on semen quality in men with inflammatory bowel disease (IBD) by a comprehensive panel of semen analyses. Methods: Semen from 40 men with IBD in remission on AZA/6-MP therapy was prospectively collected and compared with samples from 40 healthy volunteers. Paired samples (off and on AZA/6-MP) were obtained from a subset of IBD patients, and blood and semen were collected to determine 6-MP transmission to the ejaculate...
June 16, 2018: Journal of Crohn's & Colitis
A Allaoui, B Safsaf, M Tlidjane, I Djaalab, H Djaalab Mansour
Aim: The aim of the study was to assess the effect of two levels of wasted date (WD) by replacing commercial concentrate on the reproductive performance of Ouled Djellal (OD) rams. Materials and Methods: Eighteen mature (2-year-old) OD rams were equally allocated to three groups and fed during 11 weeks with one of three different experimental diets, that contained 0% (0 WD), 50% (50 WD), or 75% (75 WD) of WDs in concentrate diet. Live body weight (LBW), body condition scoring (BCS), scrotal circumference (SC), testicular weight (TW), sperm production and quality, plasma testosterone concentration (T), and sexual behavior (reaction time and number of mounts with ejaculation) were regularly recorded from every ram...
May 2018: Veterinary World
L Touazi, B Aberkane, Y Bellik, N Moula, M Iguer-Ouada
Aim: This study aimed to investigate the protective effect of Rosmarinus officinalis (L.) essential oil on rooster sperm motility during 4°C short-term storage. Materials and Methods: R. officinalis essential oil was analyzed using gas chromatography coupled to mass spectrometry to identify the active components. 10 of 45-week-old Hubbard commercial broilers were subjected to biweekly semen collections during 3 weeks. At each collection, sperm was pooled and divided into four aliquots and then diluted with Tris extender supplemented with 870, 87, or 8...
May 2018: Veterinary World
Min Xie, Khyra Sarah Utzinger, Kerstin Blickenstorfer, Brigitte Leeners
Circadian and circannual rhythms influence not only the environment, but also human physiology. In times of increasing numbers of couples struggling with infertility, and thus increasing demand for successful assisted reproduction, the aim of our study was to evaluate circadian and circannual rhythms and their association with semen quality. A total of 12 245 semen samples from 7068 men, collected at the andrology laboratory of the Department of Reproductive Endocrinology, University Hospital Zurich, between 1994 and 2015, were uniformly analysed in terms of sperm concentration, total sperm count, progressive motility and normal morphology...
June 18, 2018: Chronobiology International
F Dardmeh, H Alipour, H I Nielsen, S Rasmussen, J T Yousefi, N E Ditlevsen, H A A K T Yassin, E Morina, R K Duus, P Gazerani
Aims Sex hormones play an important role in pain in many chronic pain conditions. Relationship between chronic pain and sperm quality has not been investigated thoroughly and may provide an insight to better understanding, management and treatment of cases where chronic pain and male sub-fertility co-exist. Methods Neat (fresh semen) and processed sperm from 15 males with orthopedic chronic pain (CP) were assessed and compared with 15 healthy age matched controls. Sperm analysis was performed using the SCA computer-aided sperm analyzer...
December 29, 2017: Scandinavian Journal of Pain
Clare Nevin, Lauren McNeil, Nessar Ahmed, Chris Murgatroyd, Daniel Brison, Michael Carroll
Glycation is the non-enzymatic reaction between reducing sugars, such as glucose, and proteins, lipids or nucleic acids, producing Advanced Glycation End (AGE) products. AGEs, produced during natural senescence as well as through lifestyle factors such as diet and smoking, are key pathogenic compounds in the initiation and progression of diabetes. Importantly, many of these factors and conditions also have influence on male fertility, affecting sperm count and semen quality, contributing to the decreasing trend in male fertility...
June 13, 2018: Scientific Reports
Paula Domingo, Maite Olaciregui, Noelia González, Ignacio DE Blas, Lydia Gil
The purpose of this research was to assess whether the presence of seminal plasma (SP) can improve sperm quality of rabbit spermatozoa stored at 16ºC for 72 hours and moreover evaluate the cryoprotectant effects of glycerol, N-N-Dimethylformamide (DMF), and N-methyl-2-pyrrolidone (NMP). Semen samples were pooled and divided in eight fractions. Four of them were diluted with INRA (extender A), INRA with 6% glycerol (extender B), INRA with 6% DMF (extender C), or INRA with 6% NMP (extender D), respectively. The other four fractions were centrifuged, and the supernatant was removed in order to eliminate SP...
June 13, 2018: Experimental Animals
Dongmei Wu, Lang Chen, Fayuan Ji, Yang Si, Hongbin Sun
OBJECTIVE: To investigate the effects of antiepileptic drugs (AEDs; oxcarbazepine [OXC], levetiracetam [LEV], and lamotrigine [LTG]) on semen quality, sexual function, and sex hormones in male adults with epilepsy. METHODS: Individual treatment with OXC, LEV, or LTG was randomly assigned to 38 newly diagnosed male adult patients with epilepsy. Semen quality and sex hormones were measured before treatment and 6 months after taking the medicine. A questionnaire was administered using the International Index of Erectile Function Scale-5 and the Premature Ejaculation Diagnostic Tool Self-Assessment Scale to evaluate sexual function, followed by an analysis of the comparison between the treated patients and healthy volunteers (healthy controls) as well as the changes and differences between the patients themselves before and after treatment...
June 11, 2018: Epilepsia
Houshang Nouri, Kamal Shojaeian, Ghasem Jalilvand, Hamid Kohram
The objective was to assess the influence of pomegranate seed oil supplementation on the quality of fresh, cooled and frozen-thawed Arabian breed stallion semen. Eight stallions (n = 4 per group) received their normal diet (control group) or normal diet top dressed with 200 ml of pomegranate seed oil (PSO group). Semen was collected every fifteen days for 90 days. Stallions were reversed across the treatments after a sixty-day interval. In cooled and stored condition (2, 12 and 24 hr), spermatozoa motion characteristics, membrane integrity, viability, morphology and lipid peroxidation were analysed...
June 11, 2018: Reproduction in Domestic Animals, Zuchthygiene
Nathalie Rives, Aurélie Rives, Christine Rondanino, Mireille Castanet, Ariane Cuny, Louis Sibert
Spermatozoa have occasionally been identified in ejaculate of adult Klinefelter syndrome (KS) patients but very exceptionally in KS adolescents. Spermatozoa can also be retrieved in testicular tissue of KS adolescents. The testis may also harbor spermatogonia and noncompletely differentiated germ cells. Neither clinical features nor hormonal parameters could predict germ cell recovery in KS adults or adolescents. No predictive factors can actually demonstrate that early diagnosis of KS would allow increasing the chance of sperm retrieval even if it has been suggested that semen quality may decline with age in KS patients...
2018: Endocrine Development
Erika Aline Ribeiro Dias, Suzane Peres Campanholi, Guilherme Fazan Rossi, Camila de Paula Freitas Dell'Aqua, José Antonio Dell'Aqua, Frederico Ozanam Papa, Mariana Furtado Zorzetto, Claudia Cristina Paro de Paz, Letícia Zoccolaro Oliveira, Maria Eugênia Zerlotti Mercadante, Fabio Morato Monteiro
Semen cryopreservation comprises different steps, among them are the cooling and freezing rates which significantly influence the quality of thawed sperm. Different systems with variable freezing rates are used for freezing bull semen in the field, with a consequence of variable success rates. The objective of this study was to compare different systems for freezing bull semen in the field. Five cooling methods of semen and two methods for the subsequent freezing phase (5 × 2 factorial scheme) were used...
May 23, 2018: Animal Reproduction Science
Florencia Beracochea, María Noel Viera, Lucía Acevedo, Julián Santiago-Moreno, Rodolfo Ungerfeld
In most goat breeds, testosterone serum concentration and semen quality decrease during the nonbreeding season. However, bucks reproductive activity may be stimulated with the administration of equine chorionic gonadotropin (eCG). Therefore, the aim of this study was to determine whether the repeated administration of eCG stimulates the reproductive status of bucks during the nonbreeding season. The study was performed with 19 bucks that were assigned to a group that was treated with eCG (GeCG) and an untreated control group (GCon)...
June 8, 2018: Reproduction in Domestic Animals, Zuchthygiene
Viviane Maria Codognoto, Paulo Henrique Yamada, Rúbia Alves Schmith, Felipe Rydygier de Ruediger, Caroline Scott, Patrícia de Faria Lainetti, Suzane Brochine, Camila de Paula Freitas-Dell'Aqua, Fabiana Ferreira de Souza, Eunice Oba
The objective of the present study was to describe the proteins from the seminal plasma of buffalo and correlate these proteins with sperm motility. Ejaculates from sixteen Murrah buffalo were used. Semen collection was performed by electroejaculation, and the ejaculate was evaluated by macroscopic (volume) and microscopic analysis (subjective motility and vigor, as well as sperm concentration). After the analysis, the samples were centrifuged (800g for 10 min and 10,000 for 30 min at 4 °C), and the supernatant (seminal plasma) was used to determine total protein concentration by the Bradford method...
June 2, 2018: Animal Reproduction Science
Matteo Tesi, Chiara Sabatini, Iacopo Vannozzi, Gloria Di Petta, Duccio Panzani, Francesco Camillo, Alessandra Rota
Although semen analysis is routinely used to evaluate the male reproductive potential in the canine species, only a few authors have attempted to relate semen characteristics to donor traits, such as age or weight, and fertility. This study evaluated the effects of dogs' age (young: 0-24 months, adult: 25-84 months, old: >84 months), size (small: <15 kg, medium: 16-40 kg, large: >40 kg) and reason for semen collection (evaluation, artificial insemination, cryopreservation or research purposes) on the seminal characteristics and related the main seminal characteristics with fresh AI results...
May 17, 2018: Theriogenology
J P Kastelic, G Rizzoto, J Thundathil
Several structural and functional features keep bull testes 2°C to 6°C below body temperature, essential for the production of morphologically normal, motile and fertile sperm. The testicular vascular cone (TVC), located above the testis, consists of a highly coiled testicular artery surrounded by a complex network of small veins (pampiniform plexus). The TVC functions as a counter-current heat exchanger to transfer heat from the testicular artery to the testicular vein, cooling blood before it enters the testis...
June 2018: Animal: An International Journal of Animal Bioscience
Durgesh Murhari Golher, Arumugam Kumaresan, Kaustubh Kishor Saraf, Shivani Chhillar, Samiksha Nayak, Utkarsh K Tripathi, Chavan Nitin Bhaskar, Surender Singh Lathwal, Tushar Kumar Mohanty
Seasonality in reproduction and effects of climatic variables on testicular cytology and semen quality in bucks reared under subtropical climatic conditions were not well understood. In the present study, using testicular cytology, semen evaluation and melatonin concentrations assessed over a period of 1 year, we report that bucks reared under subtropical climatic conditions did not show seasonality in reproduction. Climatic variables including temperature, relative humidity, temperature-humidity index (THI), sunshine hours and day length were recorded daily during the whole period of experimentation (one complete year)...
June 7, 2018: International Journal of Biometeorology
Nahid Maleki-Saghooni, Khadijeh Mirzaeii, Hossein Hosseinzadeh, Ramin Sadeghi, Morvarid Irani
Objective: We performed this systematic review and meta-analysis study to determine saffron ( Crocus sativus ) effectiveness and safety in male infertility problems. Materials and Methods: The databases PubMed, Scopus, Cochrane, Google Scholar, SID, IranMedex and Magiran until July 2016 and reference section of relevant articles, were searched to find both English and Persian clinical trials on male infertility issues that used saffron as medical treatment. Also, the quality of these trials was evaluated by Oxford Center for Evidence Based Medicine checklist...
May 2018: Avicenna Journal of Phytomedicine
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