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https://www.readbyqxmd.com/read/29231961/a-mechanism-by-which-astragalus-polysaccharide-protects-against-ros-toxicity-through-inhibiting-the-protein-dephosphorylation-of-boar-sperm-preserved-at-4-%C3%A2-c
#1
Jieli Fu, Qiangzhen Yang, Yuhua Li, Peifei Li, Lirui Wang, Xinhong Li
Numerous studies have shown that Astragalus polysaccharide (APS) has strong antioxidant effects and high practical value for preserving semen at low temperatures in vitro. However, to date, little attention has been paid to the precise mechanism of APS in sperm preservation at 4 °C. Thus, to gain further insight into the protective effects of APS, the present study was performed to assess the changes in sperm quality parameters, antioxidant capacity, ATP content and protein phosphorylation levels. Here, we demonstrated that supplementation with APS could effectively preserve boar sperm quality parameters such as sperm motility, acrosome integrity and mitochondrial membrane potential...
December 12, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29231502/-clinical-observation-of-chronic-perianal-eczema-treated-with-auricular-point-sticking-therapy-and-western-medication
#2
Yong Wen, Jun Li, Qing Long, Chuan Wan, Xiaolin Lan, Ye Chen
OBJECTIVE: To observe the differences in the therapeutic effects on chronic perianal eczema between auricular point sticking therapy and auricular sticking pseudo-press therapy on the basis of the treatment with western medication. METHODS: Ninety patients were randomized into an auricular therapy group and an auricular pseudo-press therapy group (pseudo group), 45 cases in each one. The western medication was used in both of the groups. In the auricular therapy group, the auricular sticking method with semen vaccariae was added at Fengxi (SF1,zi), Shenshangxian (TG2p), Duipingjian (AT1,2,4i), Fei (CO14), Pi (CO13), Gangmen (HX5)...
June 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
https://www.readbyqxmd.com/read/29231494/-optimization-of-acupoint-application-scheme-in-the-treatment-of-bronchial-asthma-based-on-the-orthogonal-design-method
#3
Kuan Shi, Wenzhong Wu, Lanying Liu, Hesheng Wang, Dong Chen, Chengyong Liu, Cong Zhang
OBJECTIVE: To study the primary and secondary factors of the allergic history, the frequency of acupoint application and the time of acupoint application in the treatment of bronchial asthma and optimize its scheme. METHODS: Eighty patients of bronchial asthma were selected as the subjects in the orthogonal trial. The herbal medicines were the empirical formula of acupoint application (prepared at the ratio as 2:2:1:1:1:1:1:1:1 of semen brassicae, rhizome corydalis, unprocessed radix kansui, asarum sieboldii, ephedra, semen lepidii, syzygium aromaticum, cortex cinnamomi and fructus gleditsiae) and used on bilateral Feishu (BL 13), Xinshu (BL 15), Geshu (BL 17) and Shenshu (BL 23)...
June 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
https://www.readbyqxmd.com/read/29231151/in-vitro-effect-of-various-cryoprotectants-on-the-semen-quality-of-endangered-oravka-chicken
#4
Andrea Svoradová, Lenka Kuželová, Jaromír Vašíček, Andrej Baláži, Emília Hanusová, Peter Chrenek
We aimed to compare the effect of three different permeating cryoprotectants on the post-thaw spermatozoa quality. Pooled semen from Oravka cock line (n = 6) was diluted in Kobidil+ extender and frozen in cryoprotectant solutions containing 8% dimethylsulfoxide (DMSO), 8% ethylene glycol (EG) or 8% glycerol (GL) in liquid nitrogen vapours before being plunged into the liquid nitrogen. Spermatozoa motility parameters were assessed in vitro after freezing-thawing by a computer-assisted semen analysis (CASA) system and viability status was examined using fluorescent probes...
December 12, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/29230840/correlation-of-biochemical-constituents-of-seminal-plasma-with-semen-quality-in-teddy-goat-capra-hircus-bucks
#5
S Umar, M Ahmad, I Ahmad, M Zubair, Z Umar, A S Qureshi, A Manzoor, A Murtaza, A Shaukat
This study was planned to determine the relationship between semen quality parameters and the levels of biochemical constituents of seminal plasma of Teddy (Capra hircus) buck semen. For this purpose, semen ejaculates were collected from five mature healthy Teddy bucks. All the experimental bucks were kept under natural environmental conditions. Semen was collected twice in a week for the duration of 6 weeks by Artificial Vagina (AV) in the breeding season (February-April). Two successive ejaculates of single buck were pooled at time of collection, and a total of 60 semen samples were processed for semen analysis...
December 12, 2017: Andrologia
https://www.readbyqxmd.com/read/29223503/multiple-collections-of-common-carp-cyprinus-carpio-l-semen-during-the-reproductive-period-and-its-effects-on-sperm-quality
#6
Beata Irena Cejko, Beata Sarosiek, Sławomir Krejszeff, Radosław Kajetan Kowalski
Multiple collections of semen during the reproductive period of the common carp Cyprinus carpio L. were used to analyse changes in semen quality. Semen collection was performed on June 1 (first collection), 12 (second collection), and 19 (third collection) from individual males (n=11) by gentle abdominal massage. Semen quantity (semen volume and sperm count), quality (sperm motility and sperm viability), as well as seminal plasma parameters (pH of seminal plasma and seminal plasma osmotic pressure) and its enzymatic activity, e...
December 5, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29223009/influence-of-spermatozoal-lipidomic-profile-on-the-cryoresistance-of-frozen-spermatozoa-from-stallions
#7
T Cabrera, C Ramires-Neto, K R A Belaz, C P Freitas-Dell'aqua, D Zampieri, A Tata, M N Eberlin, M A Alvarenga, F F Souza
The membrane of spermatozoa, which contributes to cellular cryoresistance, contains numerous lipids with a composition that directly affects membrane fluidity and the fertilization process. In light of variations in the degree of sensitivity in equine seminal freezing, this study aimed to correlate equine semen lipids with post-thawing characteristics of spermatozoa. We used ejaculates from 34 stallions, which were evaluated (total motility ≥ 60%), frozen and thawed and reevaluated for motility of spermatozoa, membrane integrity and lipid peroxidation...
November 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/29216684/the-impact-of-corynebacterium-glucuronolyticum-on-semen-parameters-a-prospective-pre-post-treatment-study
#8
T Meštrović, B Bedenić, J Wilson, S Ljubin-Sternak, M Sviben, M Neuberg, R Ribić, G Kozina, Z Profozić
Corynebacterium glucuronolyticum (C. glucuronolyticum) is a rare isolate that is only recently being acknowledged as a potential urogenital pathogen. The bibliographical references on this bacterial species are scarce, and its influence on all semen parameters was hitherto unknown - therefore, the aim of this study was to evaluate its effects on a range of sperm quality parameters. A prospective approach to compare semen parameters before and after treatment was used in this study. C. glucuronolyticum in semen specimens was identified using analytical profile index biotyping system (API Coryne) and additionally confirmed by matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (MALDI-TOF MS), with the determination of antimicrobial susceptibility by Kirby-Bauer method...
December 7, 2017: Andrology
https://www.readbyqxmd.com/read/29215767/exposure-to-levonorgestrel-increases-nest-acquisition-success-and-decreases-sperm-motility-in-the-male-fathead-minnow-pimephales-promelas
#9
Tyler Frankel, Lance Yonkos, Franklin Ampy, Jack Frankel
Progestins are utilized as a component of human contraceptives, and enter the environment via wastewater treatment plant effluent. Certain progestins activate fish androgen receptors and cause decreases in fecundity and masculinization of females. In this study, a nest acquisition assay and computer assisted sperm analysis (CASA) was used to examine the effects of levonorgestrel (LNG) on male fathead minnow (Pimephales promelas) reproductive fitness. Males were exposed to 0, 10, or 100ng/L LNG for 14d. Combinations of a control male and a male from one of the treatments were placed into a competitive nesting assay, and time each male spent holding the nest and time spent exhibiting aggressive behaviors were analyzed at 48hrs post-exposure...
December 7, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/29214481/environmental-levels-of-triclosan-and-male-fertility
#10
Joanna Jurewicz, Michał Radwan, Bartosz Wielgomas, Paweł Kałużny, Anna Klimowska, Paweł Radwan, Wojciech Hanke
Triclosan is a synthetic chemical with broad antimicrobial activity that has been used extensively in consumer products, including personal care products, textiles, and plastic kitchenware, although the exposure which is widespread evidence from human studies is scarce. Our study aims to investigate the relationship between triclosan exposure and male fertility. Triclosan (TCS) urinary concentrations were measured using gas chromatography coupled with tandem mass spectrometry in 315 men recruited from a male reproductive health clinic with normal sperm concentration (≥ 15 mln/ml) (WHO 2010) under 45 years of age...
December 7, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29203468/edc-impact-reduced-sperm-counts-in-rats-exposed-to-human-relevant-mixtures-of-endocrine-disrupters
#11
Marta Axelstad, Ulla Hass, Martin Scholze, Sofie Christiansen, Andreas Kortenkamp, Julie Boberg
Human semen quality is declining in many parts of the world, but the causes are ill-defined. In rodents, impaired sperm production can be seen with early life exposure to certain endocrine disrupting chemicals, but the effects of combined exposures are not properly investigated. In this study, we examined the effects of early exposure to the painkiller paracetamol and mixtures of human relevant endocrine disrupting chemicals in rats. One mixture contained four estrogenic compounds, another contained eight anti-androgenic environmental chemicals, and a third mixture contained estrogens, anti-androgens and paracetamol...
December 4, 2017: Endocrine Connections
https://www.readbyqxmd.com/read/29198215/dietary-l-arginine-supplementation-improves-semen-quality-and-libido-of-boars-under-high-ambient-temperature
#12
J Q Chen, Y S Li, Z J Li, H X Lu, P Q Zhu, C M Li
l-Arginine is a nutritionally essential amino acid for spermatogenesis and plays versatile roles in animal health and can be utilized as a potential agent to improve reproductive performance of boars under high ambient temperature. The present study aimed to determine whether dietary l-arginine could alleviate heat stress-induced infertility in boars. In all, 20 boars (PIC 1040; 248.59±3.84 kg BW and 407.65±6.40 days of age) were selected and randomly assigned to four groups (group 0.0%, basal diet; group 0...
December 4, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29196809/melatonin-affects-membrane-integrity-intracellular-reactive-oxygen-species-caspase3-activity-and-akt-phosphorylation-in-frozen-thawed-human-sperm
#13
Atefeh Najafi, Emmanuel Adutwum, Abazar Yari, Ensieh Salehi, Saideh Mikaeili, Fariba Dashtestani, Farid Abolhassani, Leila Rashki, Setareh Shiasi, Ebrahim Asadi
Cryopreservation is known to induce oxidative stress in spermatozoa. Although melatonin has powerful antioxidant properties, little is known about its effects on human sperm quality during cryopreservation. The present study was undertaken to investigate the effects of melatonin treatment on human sperm parameters essential for fertilization. We first evaluated the effects of various concentrations of melatonin (0-15 mM) on human sperm parameters such as motility, viability and levels of intracellular reactive oxygen species during cryopreservation in order to identify an optimal dose with the greatest effects for further studies...
December 1, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/29193350/curcumin-nanomicelle-improves-semen-parameters-oxidative-stress-inflammatory-biomarkers-and-reproductive-hormones-in-infertile-men-a-randomized-clinical-trial
#14
Fatemeh Alizadeh, Maryam Javadi, Ali Akbar Karami, Fereshteh Gholaminejad, Maria Kavianpour, Hossein Khadem Haghighian
At least 50% of infertile couple's problems are related to male factor infertility. This Randomized, Double-Blind, Placebo-Controlled Clinical Trial conducted in Urology unit of Infertility Clinic on 60 infertile men. Patients were randomly assigned to one of intervention and placebo (n = 30) groups. Finally, 28 subjects in each group completed the study. Participants in the intervention group took daily 80 mg curcumin nanomicelle and those in the placebo group took daily placebo for 10 weeks. Semen analysis, anthropometric, physical activity assessments, total antioxidant capacity, malondialdehyde, inflammatory factors, and reproductive hormones were measured at the baseline and at the end of the study...
November 28, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/29192451/-study-on-naphthopyrone-reference-extract-and-application-on-assay-of-semen-cassiae
#15
De-Yong Zhou, Yi Luo, Lei Su, Yan-Yan Jiang, Bin Liu
A quality control method of Semen Cassiae was established by using naphthopyrone reference extract(NRE). Meanwhile, the feasibility about NRE replacing single component reference in quality control of traditional Chinese medicine was explored. After NRE of Semen Cassiae being prepared by chromatographic separation technology, we determined the three main components, cassiaside B2, rubrofusarin-6-O-β-D-gentiobioside and cassiaside C. In the meantime, an HPLC analytical method, based on the NRE as standard substance, was developed to determinate the contents of three main components in Semen Cassiae...
September 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29191079/comparison-of-semen-quality-between-university-based-and-private-assisted-reproductive-technology-laboratories
#16
Christian Fuglesang S Jensen, Omar Khan, Jens Sønksen, Mikkel Fode, James M Dupree, Tariq Shah, Dana A Ohl
OBJECTIVE: Obtaining a semen analysis (SA) is an essential step in evaluating infertile men. Despite using standardized procedures for analysis semen quality in the same individual often varies on repeated tests. The objective of this study was to investigate inter-laboratory variation in semen quality between private- and university-based assisted reproductive technology (ART) laboratories. MATERIALS AND METHODS: IRB approval was obtained to retrospectively evaluate men with a SA at both the private- and university-based ART laboratories...
December 1, 2017: Scandinavian Journal of Urology
https://www.readbyqxmd.com/read/29190351/the-associations-of-thiopurines-with-male-fertility-and-paternally-exposed-offspring-a-systematic-review-and-meta-analysis
#17
Melek Simsek, Cornelis B Lambalk, Janneke A Wilschut, Chris J J Mulder, Nanne K H de Boer
BACKGROUND: Thiopurines are widely used immunosuppressive agents. In high dosages, they inhibit the purine synthesis and are considered to be possibly harmful to spermatogenesis, and subsequently to men's fertility and their offspring. However, the clear association between thiopurine exposure and male fertility and reproduction safety, if any, is still poorly understood. OBJECTIVE AND RATIONALE: The aim of this review was to systematically summarize and meta-analyse the available data, derived from animal and human studies, regarding the influence of thiopurine exposure on fertility and conception safety in men and their offspring...
November 28, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/29184797/etiologic-classification-evaluation-and-management-of-hematospermia
#18
REVIEW
Yiji Suh, Jason Gandhi, Gunjan Joshi, Min Yea Lee, Steven J Weissbart, Noel L Smith, Gargi Joshi, Sardar Ali Khan
Hematospermia is defined by the presence of blood in the semen typically occurring in men younger than 40 years of age. Symptoms can occur due to a multitude of reasons, but are usually benign and self-limiting, requiring no additional treatment or evaluation. Despite this, the condition often impairs quality of life due to associated anxiety and must be taken seriously by the patient and the physician, particularly if recurrent, refractory, and painful. The etiology of hematospermia can be classified into inflammatory, infectious, lithiasis, cystic, obstructive, tumoral, vascular, traumatic, iatrogenic, and systemic origin...
October 2017: Translational Andrology and Urology
https://www.readbyqxmd.com/read/29184796/oncofertility-in-sarcoma-patients
#19
REVIEW
Diana M Lopategui, Raphael Yechieli, Ranjith Ramasamy
Treatment for sarcoma can significantly decrease fertility, both due to the irradiation of gonads, and the impact of chemotherapy on gametogenesis. Infertility in cancer survivors causes significant regret and decreased quality of life in their adulthood. As this cancer mainly affects children and young adults, fertility preservation is an essential part of survivorship care, however it remains one of the least implemented services in adolescent and young adult cancer patients. Success of fertility preservation is highly dependent on the referral prior to oncologic treatment...
October 2017: Translational Andrology and Urology
https://www.readbyqxmd.com/read/29184363/effect-of-antioxidants-supplementation-on-the-quality-of-beetal-buck-semen-stored-at-4%C3%A2-c
#20
Archana Sarangi, Pardeep Singh, Meenakshi Virmani, A S Yadav, Subhasish Sahu, H M Ajithakumar, Anuradha Kumari, A P Rath
Aim: An experiment was designed to evaluate the role of Vitamin E and glutathione in improving the seminal parameters during hypothermic storage of liquid semen at 4°C for 72 h. Materials and Methods: Thirty-six semen ejaculates were collected by artificial vagina from 6 bucks (Beetal) during the normal reproduction season (September to November) at weekly interval. The samples were centrifuged, and the seminal plasma was removed. The sperm pellet was diluted with Tris-based extender and divided into three groups...
October 2017: Veterinary World
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