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Semen quality

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https://www.readbyqxmd.com/read/28547739/determination-of-spermatic-vein-reflux-after-varicocele-repair-helps-to-define-the-efficacy-of-treatment-in-improving-sperm-parameters-of-subfertile-men
#1
S D'Andrea, A Micillo, A Barbonetti, A V Giordano, S Carducci, A Mancini, S Necozione, F Francavilla, S Francavilla
PURPOSE: A continuous spermatic venous reflux (SVR) at colour Doppler ultrasound (CDU) is an evidence for varicocele, a frequent correlate for male subfertility. We explored whether SVR after left varicocele repair is predictive for changes in semen quality in subfertile men. METHODS: Blood hormones (FSH, LH, and total testosterone) and scrotal CDU were obtained in subfertile patients with left grade II or grade III varicocele on physical evaluation and a poor sperm quality...
May 25, 2017: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/28545986/a-simple-field-friendly-technique-for-cryopreserving-semen-from-asian-elephants-elephas-maximus
#2
Danielle M Arnold, Charlie Gray, Terri L Roth, Sebastian Mitchell, Laura H Graham
The specific objectives of the present study were to investigate the effects of manual seeding, differing freeze and thaw rates as well as storage for 24h at 4°C prior to cryopreservation on post-thaw sperm quality in Asian elephants. Extended semen was cooled in an equitainer to 4°C, frozen in liquid nitrogen vapour at various rates with and without manual seeding or in a dry shipper and thawed at 37, 50 and 75°C. There was a significant effect of freeze rate on post-thaw motility (P<0.0001) and acrosomal integrity (P<0...
May 18, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28543808/comparison-of-two-methods-of-seminal-plasma-removal-on-buffalo-bubalus-bubalis-sperm-cryopreservation
#3
R S Albuquerque, R Morais, A N Reis, M S Miranda, Ear Dias, F M Monteiro, Ccp Paz, M Nichi, Gkv Kawai, Cpf Della'Aqua, F O Papa, R B Viana, L U Gimenes
Cryopreservation causes damage to spermatozoa, and methods minimizing this damage are therefore needed. Although much discussed, seminal plasma removal has become an alternative to improve sperm quality and viability after freezing and has been applied to different species in attempt to obtain good results. The objective of this study was to evaluate semen quality in buffaloes submitted to two methods for seminal plasma removal (filtration and centrifugation). Semen samples were collected from seven Murrah buffalo bulls (Bubalus bubalis) once a week for 8 weeks...
May 19, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28541378/what-does-a-single-semen-sample-tell-you-implications-for-male-factor-infertility-research
#4
Yu-Han Chiu, Regina Edifor, Bernard A Rosner, Feiby L Nassan, Audrey J Gaskins, Lidia Minguez-Alarcon, Paige L Williams, Cigdem Tanrikut, Russ Hauser, Jorge E Chavarro
Semen parameters are variable within-man but it is unclear whether one sample could represent a man's long-term average in epidemiologic studies. Between 2005 and 2014, 329 men from a fertility clinic provided 768 semen samples in the EARTH study in Boston, Massachusetts.Total sperm count, concentration, morphology, motility, and ejaculate volume were assessed. We used linear mixed models to compare men's first samples to their long-term averages, and calculate intraclass correlation coefficients for each parameter...
May 24, 2017: American Journal of Epidemiology
https://www.readbyqxmd.com/read/28537816/effect-of-vitamin-e-and-polyunsaturated-fatty-acids-on-cryopreserved-sperm-quality-in-bos-taurus-bulls-under-testicular-heat-stress
#5
João D A Losano, Daniel S R Angrimani, Andressa Dalmazzo, Carolina C Rocha, Maíra M Brito, Eduardo G A Perez, Roberta H Tsunoda, Paola A A Góes, Camilla M Mendes, Mayra E O A Assumpção, Valquiria H Barnabe, Marcilio Nichi
Taurine bulls are highly susceptible to heat stress, leading to increased oxidative stress (OS) and impaired sperm viability. Polyunsaturated fatty acids (PUFAs) supplementation can be an alternative to improve semen quality, which also results in more sperm susceptibility to lipid peroxidation. Moreover, this deleterious effect can be exacerbated in animals affected by heat stress. Vitamin E is a key antioxidant that counteracts lipid peroxidation of sperm membrane caused by OS. Thus, combining PUFAs with vitamin E may improve sperm quality...
May 24, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28535997/sperm-sexing-in-nili-ravi-buffalo-through-modified-swim-up-validation-using-sybr-%C3%A2-green-real-time-pcr
#6
Asma-Ul-Husna, Muhammad Amjad Awan, Abid Mehmood, Tasawar Sultana, Qaisar Shahzad, Muhammad Sajjad Ansari, Bushra Allah Rakha, S M Saqlan Naqvi, Shamim Akhter
Sperm sexing through flow-sorting technology is relatively expensive, requires considerable technical support and is actually not practicable in many developing countries. The aim of this study was to investigate the feasibility of producing enriched pools of X or Y chromosome-bearing sperm by a modified swim-up method. For this purpose semen was collected from five mature Nili-Ravi buffalo bulls for a period of six weeks. The qualifying ejaculates were divided into two aliquots for further processing through modified swim-up or control (untreated)...
May 6, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28535305/preservation-of-domesticated-honey-bee-hymenoptera-apidae-drone-semen
#7
M Paillard, A Rousseau, P Giovenazzo, J L Bailey
Preservation of honey bee (Apis mellifera L., Hymenoptera: Apidae) sperm, coupled with instrumental insemination, is an effective strategy to protect the species and their genetic diversity. Our overall objective is to develop a method of drone semen preservation; therefore, two experiments were conducted. Hypothesis 1 was that cryopreservation (-196 °C) of drone semen is more effective for long-term storage than at 16 °C. Our results show that after 1 yr of storage, frozen sperm viability was higher than at 16 °C, showing that cryopreservation is necessary to conserve semen...
May 23, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28534278/the-effect-of-antioxidant-agents-addition-and-freezing-method-on-quality-parameters-of-frozen-thawed-ram-semen
#8
Leonidas Vichas, Ioannis A Tsakmakidis, Dimitrios Vafiadis, Georgios Tsousis, Eleni Malama, Constantin M Boscos
The aim of this study was to evaluate the effect of antioxidant agents and freezing methods on the ability of ram sperm to preserve its post-thaw quality characteristics. Six Chios rams were subjected to 52 weekly semen collections. Each ram was used as semen donor for freezing experiments once every 2 weeks. Equal number of good quality spermatozoa from each ejaculate (concentration ≥1 × 10(9) spermatozoa/ml, motility ≥70%, motility score ≥3.5) were pooled. Three equal aliquots of the pooled sample were diluted using three different fractions of a milk-based and glycerol extender (control, quercetin-enriched, α-tocopherol-enriched)...
May 22, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28532825/growth-performance-reproductive-traits-and-offspring-survivability-of-genetically-modified-rams-overexpressing-toll-like-receptor-4
#9
Yu-Chang Yao, Hong-Bing Han, Xu-Ting Song, Shou-Long Deng, Yu-Feng Liu, Ming-Hai Lu, Yun-Hai Zhang, Mei-Yu Qi, Hai-Juan He, Su-Mei Wang, Guo-Shi Liu, Wu Li, Zheng-Xing Lian
Genetic modification provides a means to enhancing disease resistance in animals. Toll-like receptor 4 (TLR4), a member of the TLR family, is critical for the recognition of lipopolysaccharide (LPS)/endotoxin from Gram-negative bacteria by host immune cells, which initiates cell activation and subsequently triggers a proinflammatory response to the invading pathogens. In this study, the first generation of genetically modified (GM) sheep overexpressing TLR4 was produced by microinjection for better disease resistance...
July 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28531319/improved-sperm-kinematics-in-semen-samples-collected-after-2-h-versus-4-7-days-of-ejaculation-abstinence
#10
H Alipour, G Van Der Horst, O B Christiansen, F Dardmeh, N Jørgensen, H I Nielsen, C Hnida
STUDY QUESTION: Does a short abstinence period of only 2 h yield spermatozoa with better motility characteristics than samples collected after 4-7 days? SUMMARY ANSWER: Despite lower semen volume, sperm concentration, total sperm counts and total motile counts, higher percentages of motile spermatozoa with higher velocity and progressiveness were detected in samples obtained after 2 h. WHAT IS KNOWN ALREADY: Most studies that have assessed the effect of abstinence periods on sperm motility parameters in men with a sperm concentration below 15 million/ml have detected a higher percentage of motile spermatozoa in samples obtained after short abstinence periods...
May 22, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28523277/the-effect-of-l-carnitine-hypotaurine-and-taurine-supplementation-on-the-quality-of-cryopreserved-chicken-semen
#11
Agnieszka Partyka, Olga Rodak, Joanna Bajzert, Joanna Kochan, Wojciech Niżański
The objective of this study was to investigate the effect of L-carnitine (LC), hypotaurine (HT), and taurine (T) on the quality of frozen-thawed chicken semen. Pooled semen samples were divided into seven aliquots (control, 1 mM LC, 5 mM LC, 1 mM HT, 10 mM HT, 1 mM T, and 10 mM T) and subjected to cryopreservation. Postthaw sperm motility was determined by IVOS system and sperm characteristics were assessed with fluorochromes and flow cytometry. The highest sperm motility and the highest percentage of viable sperm were in the HT1 group (P < 0...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28522219/racial-variation-in-semen-quality-at-fertility-evaluation
#12
Yash S Khandwala, Chiyuan A Zhang, Shufeng Li, Barry Behr, David Guo, Michael L Eisenberg
OBJECTIVE: To identify racial differences in semen quality among men living in the same geographic area seeking fertility evaluation. METHODS: Men obtaining a semen analysis for infertility evaluation or treatment between 2012 and 2016 at a single center were identified and demographic data including height, weight, BMI and age were described. Mean semen parameters and the proportions of men with suboptimal parameters based on the WHO 5(th) edition criteria were also compared based on race...
May 15, 2017: Urology
https://www.readbyqxmd.com/read/28521575/high-frequency-of-de-novo-daz-microdeletion-in-sperm-nuclei-of-subfertile-men-possible-involvement-of-genome-instability-in-idiopathic-male-infertility
#13
Hossein Mozdarani, Pegah Ghoraeian, Sohail Mozdarani, Parvin Fallahi, Anahita Mohseni-Meybodi
The occurrence and diagnosis of Y-chromosome microdeletions, specifically deletions of the DAZ (Deleted in Azoospermia) genes are an important issue in male infertility. Screening Y chromosome microdeletion is mainly done using polymerase chain reaction (PCR) on blood leukocytes. However, there is some evidence indicating that presence of DAZ in somatic cells might not be indicative of its presence in the germ cell lineage. Therefore, a total of 130 men with poor semen quality were examined for presence of DAZ microdeletion in their leukocytes...
May 19, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28520588/evaluation-of-the-quality-of-semen-and-sexual-function-in-men-with-inflammatory-bowel-disease
#14
Paz Valer, Alicia Algaba, Daniel Santos, Manuel E Fuentes, Elva Nieto, Javier P Gisbert, Pilar López, Elvira Quintanilla, Francisco J García-Alonso, Iván Guerra, Álvaro Páez, Fernando Bermejo
BACKGROUND: Data on fertility rates and medication safety in men with inflammatory bowel disease (IBD) are limited. The aim of this study was to evaluate whether there is a seminal alteration in patients with IBD and, if so, to evaluate the mechanisms that may play a role according to what has been described in the literature. Its secondary aim was to evaluate the impact on male sexual function of IBD. METHODS: Multicenter, cross-sectional, case series study comparing men with IBD and control subjects...
May 16, 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/28517368/testicular-thermoregulation-scrotal-surface-temperature-patterns-and-semen-quality-of-water-buffalo-bulls-reared-in-a-tropical-climate
#15
L K X Silva, J S Sousa, A O A Silva, J B Lourenço Junior, C Faturi, L G Martorano, I M Franco, M H A Pantoja, D V Barros, A R Garcia
This study evaluated the capacity of thermoregulation and its consequences on the scrotal surface temperature patterns and semen quality of buffalo bulls raised in a wet tropical climate. Eleven water buffaloes were evaluated in the rainiest, in the transitional and in the less rainy season. Air temperature and humidity were consistently high, but the animals did not show thermal stress in any season. The scrotal temperature gradient of buffalo bulls using infrared thermography was described, and three parallel and decreasing thermal bands were characterised...
May 18, 2017: Andrologia
https://www.readbyqxmd.com/read/28517043/sperm-chromatin-quality-and-dna-integrity-in-partial-versus-total-globozoospermia
#16
A R Talebi, J Ghasemzadeh, M A Khalili, I Halvaei, F Fesahat
Globozoospermia is a severe form of teratozoospermia with low incidence in infertile patients, considered as one of the important causes of male infertility. The objective was to investigate the chromatin/DNA integrity as well as apoptosis in ejaculated spermatozoa of cases with partial or total globozoospermia. Fifty-seven semen samples were divided into three groups of partial globozoospermia (n = 17), total globozoospermia (n = 10) and normozoospermia (control; n = 30). Sperm chromatin condensation, DNA integrity and apoptosis were assessed using cytochemical assays...
May 18, 2017: Andrologia
https://www.readbyqxmd.com/read/28511996/comparative-characteristics-of-spermatozoa-harvested-and-cryopreserved-in-culture-and-cryoprotectant-media-with-or-without-donor-serum-proteins
#17
Ghofraan A Ata'Allah, Noor Azmi Mat Adenan, Nuguelis Razali, Kannappan Palaniappan, Rosliza Saad, Siti Khadijah Idris, Krishnan Kanniah, Jaffar Ali
The objectives of this study is to evaluate the efficacy of protein-free media in the preparation, holding and crypreservation of spermatazoa for use in ART. Normozoospermic semen samples (N=71) were used to compare the effects of media on the survival and quality of spermatozoa when washed and cultured with different media with and without added proteins at 4°C, 15°C, 22°C and 37°C for 0, 4-7 and 24h. Survival and quality of spermatozoa were assessed after freeze-thaw with synthetic cryoprotectant with and without proteins...
May 13, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28511862/effect-of-different-concentrations-of-egg-yolk-and-virgin-coconut-oil-in-tris-based-extenders-on-chilled-and-frozen-thawed-bull-semen
#18
A A Tarig, H Wahid, Y Rosnina, N Yimer, Y M Goh, F H Baiee, A M Khumran, H Salman, M Ebrahimi
The aim of this study was to evaluate the effects of 8% virgin coconut oil (VCO) combined with different percentages of egg yolk in Tris extender on the quality of chilled and frozen-thawed bull semen. A total of 24 ejaculates from four bulls were collected using an electroejaculator. Semen samples were diluted with 8% VCO in Tris extender which contained different concentrations 0% (control), 4%, 8%, 12%, 16% and 20% egg yolk. The diluted semen samples were divided into two fractions: one was chilled and stored at 4°C until evaluation after 24, 72, and 144h; the second fraction was processed by chilling for 3h at 4°C to equilibrate, then packaged in 0...
April 10, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28505938/re-cancer-risk-in-first-and-second-degree-relatives-of-men-with-poor-semen-quality
#19
Craig Niederberger
No abstract text is available yet for this article.
June 2017: Journal of Urology
https://www.readbyqxmd.com/read/28501790/obesity-aggravates-toxic-effect-of-bpa-on-spermatogenesis
#20
Weiyue Hu, Tianyu Dong, Lingling Wang, Quanquan Guan, Ling Song, Daozhen Chen, Zuomin Zhou, Minjian Chen, Yankai Xia, Xinru Wang
Both bisphenol A (BPA) and obesity affect male reproductive system. However, whether there is an interaction between them remains poorly understood. The aim of the present study was to evaluate the interaction between BPA exposure and obesity on semen quality and elucidate the mechanism in humans and animals. We firstly analyzed the interaction on semen volume, sperm count per ejaculate, sperm concentration and sperm motility in 357 men, and found that urinary BPA concentration was significantly correlated with sperm count per ejaculate in obese men (β=-34...
May 11, 2017: Environment International
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