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https://www.readbyqxmd.com/read/29678467/effect-of-achillea-millefolium-loaded-nanophytosome-in-the-post-thawing-sperm-quality-and-oxidative-status-of-rooster-semen
#1
Davood Najafi, Ramezan Ali Taheri, Abouzar Najafi, Abbas Abbas Rouhollahi, Manuel Alvarez-Rodriguez
The aim of this study was to compare the effectiveness of antioxidants including Achillea millefolium extract (AmE) (n0t1.5: 1.5, n0t3: 3 and n0t4.5: 4.5 mg/L) and AmE loaded in nano phytosome (n1t1.5: 1.5, n1t3: 3 and n1t4.5: 4.5 mg/L) in the freezing of Ross 308 rooster semen. Sperm motility (CASA), membrane integrity (HOS test), viability, total abnormality and enzymatic parameters (SOD, CAT and GPx) were assessed after thawing. AmE-loaded nano phytosome at a concentration of 3 mg/l resulted in significantly (P < 0...
April 17, 2018: Cryobiology
https://www.readbyqxmd.com/read/29676290/increased-expression-of-pelp1-in-human-sperm-is-correlated-with-decreased-semen-quality
#2
Izabela Skibinska, Miroslaw Andrusiewicz, Michal Soin, Magdalena Jendraszak, Paulina Urbaniak, Piotr Jedrzejczak, Malgorzata Kotwicka
Proline-, glutamic acid-, and leucine-rich protein 1 (PELP1) is a scaffolding protein involved in both genomic and nongenomic estrogen signal transduction pathways. To date, the role of PELP1 protein has yet to be characterized in human sperm and has not been associated with sperm parameters. To confirm the presence of PELP1 in human sperm, fresh semen samples were obtained from 178 donors. The study was designed to establish both mRNA and protein presence, and protein cellular localization. Additionally, the number of PELP1-positive spermatozoa was analyzed in men with normal and abnormal semen parameters...
April 20, 2018: Asian Journal of Andrology
https://www.readbyqxmd.com/read/29676088/-explore-microcosmic-connection-between-autophagy-mechanism-and-follicular-development-based-on-kidney-governing-reproduction-theory
#3
REVIEW
Jun Bai, Ke-Ming Wu, Ran-Ran Gao
In the theory of traditional Chinese medicine(TCM) that "kidney storing essence and governing reproduction", reproductive essence is an important part of the kidney essence and acts as the original material of offspring embryos. Sperm, oocyte and zygote should be all included in the range of reproductive essence. Ovum is the essence of reproduction from inborn. The follicles maturation depends on the quality of oocyte and the vigor of kidney essence. Meanwhile, discharge of mature ovum relies on the stimulation and promotion by kidney Qi...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29673661/vitamin-c-attenuates-negative-effects-of-vitrification-on-sperm-parameters-chromatin-quality-apoptosis-and-acrosome-reaction-in-neat-and-prepared-normozoospermic-samples
#4
Esmat Mangoli, Ali Reza Talebi, Morteza Anvari, Fatemeh Taheri, Mahboobeh Vatanparast, Tahereh Rahiminia, Akram Hosseini
OBJECTIVE: Aim of this study was to evaluate the effects of vitamin C on sperm parameters, sperm chromatin quality and apoptosis resulted of vitrification in neat semen and prepared spermatozoa of normozoospermic samples. MATERIAL AND METHODS: Forty semen samples from normozoospermic men were included in this prospective study. Each sample was divided into five groups. Group I: control or fresh semen, group II: semen prepared by swim-up method and then vitrified, group III: neat semen was vitrified, group IV: vitamin C (600 μM) was added to prepared spermatozoa and then vitrified and group V: vitamin C (600 μM) was added to neat semen and then vitrified...
April 2018: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29673083/fine-particle-matter-disrupts-the-blood-testis-barrier-by-activating-tgf-%C3%AE-3-p38-mapk-pathway-and-decreasing-testosterone-secretion-in-rat
#5
Jianhui Liu, Lihua Ren, Jialiu Wei, Jin Zhang, Yupeng Zhu, Xiangyang Li, Li Jing, Junchao Duan, Xianqing Zhou, Zhiwei Sun
Fine particle matter (PM) is correlated with male reproductive dysfunction in animals and humans, but the underlying mechanisms remain unknown. To investigate the toxic mechanism of PM, 32 male Sprague-Dawley (SD) rats were exposed to saline or PM2.5 with the doses of 1.8, 5.4, and 16.2 mg/kg.b.w. via intratracheal instillation, respectively, one time every 3 days, in total times for 30 days. Sperm concentration, hormone level, the expressions of BTB-associated protein and the mitogen-activated protein kinase (MAPK) pathway, tumor necrosis factor α and transforming growth factor β3 levels were detected...
April 19, 2018: Environmental Toxicology
https://www.readbyqxmd.com/read/29672007/how-general-semen-quality-influences-the-blastocyst-formation-rate-analysis-of-4205-ivf-cycles
#6
Mariana M Piccolomini, Tatiana Cs Bonetti, Eduardo La Motta, Paulo C Serafini, Jose R Alegretti
OBJECTIVE: To select embryos with higher implantation potential, the extended culture has been the most frequently applied strategy worldwide, and consequently leads to higher live birth rates per transfer. Sperm quality is a determining feature, and it may influence the outcomes of IVF from fertilization to embryo development. Therefore, we hypothesize that blastocyst formation may also be impaired by general semen quality. METHODS: We analyzed 4205 IVF cycles...
April 19, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29670898/effects-of-withania-somnifera-on-reproductive-system-a-systematic-review-of-the-available-evidence
#7
REVIEW
Ramin Nasimi Doost Azgomi, Afshar Zomorrodi, Hossein Nazemyieh, Seyed Mohammad Bagher Fazljou, Homayoun Sadeghi Bazargani, Fatemeh Nejatbakhsh, Arezoo Moini Jazani, Yadollah Ahmadi AsrBadr
Introduction: Withania somnifera (WS) also known as ashwagandha is a well-known medicinal plant used in traditional medicine in many countries for infertility treatment. The present study was aimed at systemically reviewing therapeutic effects of WS on the reproductive system. Methods: This systematic review study was designed in 2016. Required data were obtained from PubMed, Scopus, Google Scholar, Cochrane Library, Science Direct, Web of Knowledge, Web of Science, and manual search of articles, grey literature, reference checking, and expert contact...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29670053/testosterone-dependent-mir-26a-5p-and-let-7g-5p-act-as-signaling-mediators-to-regulate-sperm-apoptosis-via-targeting-pten-and-pmaip1
#8
Jideng Ma, Yu Fan, Jinwei Zhang, Siyuan Feng, Zihui Hu, Wanling Qiu, Keren Long, Long Jin, Qianzi Tang, Xun Wang, Qi Zhou, Yiren Gu, Weihang Xiao, Lingyan Liu, Xuewei Li, Mingzhou Li
Recent evidence suggests that testosterone deficiency can dramatically decrease the quality of sperm. MicroRNAs (miRNAs) are conserved mediators of post-transcriptional gene regulation in eukaryotes. However, the systemic regulation and function of miRNAs in sperm quality decline induced by testosterone deficiency has not been investigated. Here, we found that the sperm apoptosis was significantly enhanced and the sperm motility was dramatically decreased in hemicastrated pigs. We then used small RNA sequencing to detect miRNA profiles of sperm from pigs with prepubertal hemicastration (HC) and compared them with control libraries...
April 18, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29665384/cryopreservation-of-sperm-bundles-spermatozeugmata-from-endangered-livebearing-goodeids
#9
Yue Liu, Leticia Torres, Terrence R Tiersch
More than half of fishes in the family Goodeidae are considered to be endangered, threatened, or vulnerable. Sperm cryopreservation is an effective tool for conserving genetic resources of imperiled populations, but development of protocols with livebearing fishes faces numerous challenges including the natural packaging of sperm into bundles. In this study the cryopreservation of sperm bundles (spermatozeugmata) of three goodeids species was evaluated. Sperm quality was evaluated by activation with NaCl-NaOH solution (at 300 mOsmol/kg and pH 11...
April 14, 2018: Cryobiology
https://www.readbyqxmd.com/read/29665058/testicular-germ-cell-apoptosis-and-sperm-defects-in-mice-upon-long-term-high-fat-diet-feeding
#10
Songita Ghosh, Sutapa Mukherjee
The growing prevalence of male infertility is a matter of serious concern. One of the putative causes being nutritional excess from continuous consumption of high fat diet (HFD) leading to insulin resistance albeit the specific relationship is not fully understood. Besides, there are many contradictions in the available literature on the subject. Therefore, we sought to characterize in detail the effects of HFD upon testicular function and sperm quality in mice with particular focus on isolated testicular germ cells and spermatozoa, respectively...
April 17, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29664790/should-we-consider-telomere-length-and-telomerase-activity-in-male-factor-infertility
#11
Carlos Balmori, Elisa Varela
PURPOSE OF REVIEW: The purpose of this review is to analyze what is known to date about the relation between telomeres and male fertility, and if it is possible for telomeres, or elements related to them, to be used as new prognostic biomarkers in fertility treatment. RECENT FINDINGS: Cells in germ series, including spermatozoids, have longer telomeres (10-20 kb), and do not seem to undergo the shortening that takes place in somatic cells with age as they present telomerase activity...
April 16, 2018: Current Opinion in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29659832/average-sperm-count-remains-unchanged-despite-reduction-in-maternal-smoking-results-from-a-large-cross-sectional-study-with-annual-investigations-over-21-years
#12
L Priskorn, L Nordkap, A K Bang, M Krause, S A Holmboe, D L Egeberg Palme, S B Winge, N Mørup, E Carlsen, U N Joensen, M Blomberg Jensen, K M Main, A Juul, N E Skakkebaek, T K Jensen, N Jørgensen
STUDY QUESTION: How are temporal trends in lifestyle factors, including exposure to maternal smoking in utero, associated to semen quality in young men from the general population? SUMMARY ANSWER: Exposure to maternal smoking was associated with lower sperm counts but no overall increase in sperm counts was observed during the study period despite a decrease in this exposure. WHAT IS KNOWN ALREADY: Meta-analyses suggest a continuous decline in semen quality but few studies have investigated temporal trends in unselected populations recruited and analysed with the same protocol over a long period and none have studied simultaneous trends in lifestyle factors...
April 11, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29656391/proteomic-analysis-of-sperm-proteins-in-infertile-men-with-high-levels-of-reactive-oxygen-species
#13
A Ayaz, A Agarwal, R Sharma, N Kothandaraman, Z Cakar, S Sikka
Oxidative stress is a significant risk factor for male infertility. A pro-oxidant testicular environment may alter the expression profile of functional sperm proteins and result in poor sperm quality. Patients and donors were divided into ROS (-) and ROS (+) groups. Using computational studies, and data mining of available literature on spermatozoa, oxidative stress and proteomics, we identified three core regulatory proteins angiotensin-converting enzyme (ACE), heat-shock protein (Hsp70) family A member 2 (HSPA2) and ribosomal protein subunit 27A (RPS27A) and seven interlink proteins NOS2, SUMO2, UBL4A, FBXO25, MAP3K3, APP and UBC...
April 15, 2018: Andrologia
https://www.readbyqxmd.com/read/29655402/determining-the-relationship-between-bull-sperm-kinematic-subpopulations-and-fluorescence-groups-using-an-integrated-sperm-quality-analysis-technique
#14
J L Yániz, I Palacín, K S Caycho, C Soler, M A Silvestre, P Santolaria
The aim of the present study was to determine whether there is an association between the kinematic sperm subpopulations and fluorescent groups in bulls using a new fluorescent staining method that allows classification of spermatozoa into groups depending on their acrosomal and membrane integrity, as well as functional status, without inhibiting sperm motility. Cryopreserved semen samples from 10 Holstein bulls were used in the study. A multiparametric analysis of results obtained by the ISAS 3Fun kit (Proiser) was performed...
April 16, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29653389/how-does-the-microbial-load-affect-the-quality-of-equine-cool-stored-semen
#15
E Varela, J Rey, E Plaza, P Muñoz de Propios, J M Ortiz-Rodríguez, M Álvarez, L Anel-López, L Anel, P De Paz, M C Gil, J M Morrell, C Ortega-Ferrusola
Contaminating bacteria present in stallion ejaculates may compromise sperm quality during storage. Different procedures have been used to reduce the load of microorganisms in semen and avoid bacterial growth during storage. The aims of this study were: 1) to evaluate different techniques to eliminate bacteria in semen 2) to study the relationship between total microflora load (TML) and ROS production; and 3) to determine if TML affects the functionality of cool-stored sperm. Ejaculates from 11 stallions were split and processed in 3 ways: A...
March 26, 2018: Theriogenology
https://www.readbyqxmd.com/read/29651197/a-strategy-for-sperm-cryopreservation-of-atlantic-salmon-salmo-salar-for-remote-commercial-scale-high-throughput-processing
#16
Huiping Yang, E Hu, John T Buchanan, Terrence R Tiersch
Sperm cryopreservation is an essential tool for long-term storage of genetic resources for aquaculture fishes. The goal of this study was to develop an efficient and streamlined protocol for high-throughput processing for sperm cryopreservation in Atlantic salmon, Salmo salar . The objectives were to evaluate: 1) osmolality of blood serum for determining extender osmolality; 2) effects of extenders for fresh sperm dilution and refrigerated storage; 3) effects of methanol and dimethyl sulfoxide (DMSO) on fresh sperm motility, and 4) motility and fertility after thawing...
February 2018: Journal of the World Aquaculture Society
https://www.readbyqxmd.com/read/29650062/fish-sperm-motility-analysis-the-central-role-of-the-flagellum
#17
Sergii Boryshpolets, Vitaliy Kholodnyy, Jacky Cosson, Borys Dzyuba
Motility analysis of spermatozoa relies on the investigation of either head trajectories or flagellum characteristics. Those two sets of parameters are far from being independent, the flagellum playing the role of motor, whereas the head plays a passive role of cargo. Therefore, quantitative descriptions of head trajectories represent a simplification of the complex pattern of whole sperm cell motion, resulting from the waves developed by the flagellum. The flagellum itself responds to a large variety of signals that precisely control its axoneme to allow activation, acceleration, slowing down or reorientation of the whole spermatozoon...
April 13, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29649854/effect-of-superoxide-dismutase-catalase-and-glutathione-peroxidase-supplementation-in-the-extender-on-chilled-semen-of-fertile-and-hypofertile-dogs
#18
Chiara Del Prete, Francesca Ciani, Simona Tafuri, Maria Pia Pasolini, Giovanni Della Valle, Veronica Palumbo, Lucia Abbondante, Antonio Calamo, Vincenza Barbato, Roberto Gualtieri, Riccardo Talevi, Natascia Cocchia
This study investigated the correlation between oxidative stress status and main canine sperm parameters and the effect of addition of Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione Peroxidase (GPx) combination in egg yolk tris-citrate glucose (EYT-G) extender on semen during 10 days of storage at 4° C. Ten Boxer dogs were included in this study and divided in two groups, fertile (F) and hypofertile (H), depending on pregnancy and live birth rates in the previous year. The semen evaluation was performed at the day of collection (D0) and after 5 (D5) and 10 (D10) days of cooled storage at 5° C...
April 12, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29644166/electroejaculation-combined-with-assisted-reproductive-technology-in-psychogenic-anejaculation-patients-refractory-to-penile-vibratory-stimulation
#19
Xianghu Meng, Longchang Fan, Tao Wang, Shaogang Wang, Zengjun Wang, Jihong Liu
Background: Psychogenic anejaculation (PAE) is a rare disease in clinic seriously affecting quality of life, especially fertility of patients. However, there is not an effective treatment for these patients. The aim of the study was to evaluate the results of treatment with electroejaculation (EEJ), intrauterine insemination (IUI), and intracytoplasmic sperm injection (ICSI) in patients with PAE who were refractory to penile vibratory stimulation (PVS). Methods: Twenty PVS-refractory patients with PAE were included in the retrospective study...
March 2018: Translational Andrology and Urology
https://www.readbyqxmd.com/read/29629734/changes-of-sperm-parameters-along-time-among-groups-of-different-qualities
#20
Shimi Barda, Tomer Bar-Noy, Amnon Botchan, Ofer Lehavi, Haim Yavetz, Ron Hauser
BACKGROUND: Male infertility is solely responsible for approximately 20% of all infertility in couples. Various factors have been proposed as having a negative effect on sperm quality; however, the reasons for the global decline in sperm parameters during the last few decades are still controversial. OBJECTIVES: To investigate the fluctuations of semen parameters (sperm concentration, motility, and morphology) in three sperm quality groups and to examine the trends of those parameters in the same men over time...
April 2018: Israel Medical Association Journal: IMAJ
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