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https://www.readbyqxmd.com/read/28217884/male-age-and-strain-affect-ejaculate-quality-in-the-mexican-fruit-fly
#1
Mariana Herrera-Cruz, Solana Abraham, Nicolas Nuñez-Beverido, Norma Flores-Estévez, Martha Reyes-Hernández, Mayvi Alvarado, Diana Pérez-Staples
Aging in all organisms is inevitable. Male age can have profound effects on mating success and female reproduction, yet relatively little is known on the effects of male age on different components of the ejaculate. Furthermore, in mass-reared insects used for the Sterile Insect Technique, there are often behavioral differences between mass-reared and wild males, while differences in the ejaculate have been less studied. The ejaculate in insects is composed mainly of sperm and accessory gland proteins. Here, we studied how male age and strain affected (i) protein quantity of testes and accessory glands, (ii) the biological activity of accessory gland products injected into females, (iii) sperm viability and (iv) sperm quantity stored by females in wild and mass-reared Anastrepha ludens (Diptera: Tephritidae)...
February 19, 2017: Insect Science
https://www.readbyqxmd.com/read/28216914/the-quality-of-sperm-preparation-medium-affects-the-motility-viability-and-dna-integrity-of-human-spermatozoa
#2
Fatemeh Anbari, Iman Halvaei, Ali Nabi, Shahin Ghazali, Mohammad Ali Khalili, Lars Johansson
AIM: The goal was to compare the effects of three different sperm preparation media on sperm motility, viability, and DNA integrity of semen samples from normozoospermic men. METHODS: A total of 15 normozoospermic males were included in the study. The semen analysis (SA) was performed in accordance with the WHO guidelines (2010). After SA, each sample was divided into three aliquots, and swim-up was performed with three different sperm preparation media (Sperm Preparation Media, Origio, Denmark; Ham's F10, Biochrome, Berlin, Germany; and VitaSperm™, Innovative Biotech, Iran)...
October 2016: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/28215683/effects-of-hormonal-stimulation-on-the-concentration-and-quality-of-excreted-spermatozoa-in-the-critically-endangered-panamanian-golden-frog-atelopus-zeteki
#3
Gina Della Togna, Vance L Trudeau, Brian Gratwicke, Matthew Evans, Lauren Augustine, Han Chia, Edward J Bronikowski, James B Murphy, Pierre Comizzoli
Knowledge of basic gamete biology is critical to better protect and propagate endangered amphibian species and also to develop reproductive technologies combined with germplasm cryopreservation. The objectives of the study were to test different hormonal stimulations and then characterize the quantity and quality of Panamanian golden frog (Atelopus zeteki) spermatozoa. Following intraperitoneal injection of the gonadotropin-releasing hormone agonist (des-Gly(10), D-Ala(6), Pro-NHEt(9-)-GnRH 1, 2 or 4 μg/g of body weight), human chorionic gonadotropin (hCG; 5 or 10 IU/gbw), or Amphiplex™ (0...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28215681/sperm-evaluation-of-jungle-cat-felis-chaus-obtained-by-urethral-catheterization-ct-after-medetomidine-administration
#4
M S Kheirkhah, M Mollapour Sisakht, M Mohammadsadegh, H R Moslemi
This study aimed to evaluate semen from Jungle Cat (Felis chaus) by urethral catheterization (CT) after medetomidine administration that offers feasible and different approaches to obtaining good quality sperm, especially in wild felids. Accordingly, this method was tested in five Jungle Cats. After general anesthesia with the α2-agonist medetomidine (which also stimulates semen release into the urethra) and ketamine, an abdomen ultrasound was performed to locate dilation of the first segment of the urethra (prostatic urethra)...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28215454/sperm-quality-and-oxidative-status-as-affected-by-homogenization-of-liquid-stored-boar-semen-diluted-in-short-and-long-term-extenders
#5
Mariana B Menegat, Ana Paula G Mellagi, Rafael C Bortolin, Tila A Menezes, Amanda R Vargas, Mari Lourdes Bernardi, Ivo Wentz, Daniel P Gelain, José Cláudio F Moreira, Fernando P Bortolozzo
Homogenization of diluted boar semen during storage has for a long time been regarded as beneficial. Recent studies indicated an adverse effect of homogenization on sperm quality for yet unknown reasons. This study aimed to verify the effect of homogenization on sperm parameters and to elucidate the impact of oxidative stress. Twenty-one normospermic ejaculates (21 boars) were diluted with Androstar(®) Plus (AND) and Beltsville Thawing Solution (BTS). Semen doses were submitted to no-homogenization (NoHom) or twice-a-day manual homogenization (2xHom) during storage at 17°C for 168h...
February 12, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28214572/clinical-relevance-of-oxidation-reduction-potential-in-the-evaluation-of-male-infertility
#6
Ashok Agarwal, Siew May Wang
Objectives To evaluate 1) the relationship between oxidation-reduction potential (ORP) and abnormal sperm quality, 2) changes in ORP and sperm parameters over time, in search of a potential surrogate marker of poor sperm quality that may assist in the diagnosis of oxidative stress- related male infertility. Methods 194 infertile men were included and 28 patients were identified to have repeated semen analyses and ORP measurements. The semen samples obtained were categorized into normal and abnormal sperm parameters based on the World Health Organization (WHO) 5(th) edition guidelines...
February 15, 2017: Urology
https://www.readbyqxmd.com/read/28214066/identification-of-g-170g-a-and-g-332g-a-mutations-in-exon-3-of-leptin-gene-bcnl-and-cail-and-their-association-with-semen-quality-and-testicular-dimensions-in-sanjabi-rams
#7
Roya Bakhtiar, Alireza Abdolmohammadi, Hadi Hajarian, Zahra Nikousefat, Davood Kalantar-Neyestanaki
The purpose of this study was to investigate leptin gene polymorphisms and their relationships with the characteristics of sperm quality and testicular dimensions. Semen samples were collected from 96 Sanjabi rams during autumn and spring seasons over two years. Simultaneously, the dimensions of length, width and scrotal circumference were measured. Blood samples were taken from the jugular vein to extract DNA. PCR was performed to amplify a 463bp fragment including exon 3 of leptin gene. PCR products were digested by Bcnl and Cail restriction enzymes to identify 170G>A and 332G>A mutations in exon 3, respectively...
February 4, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28208955/high-serum-estradiol-and-heavy-metals-responsible-for-human-spermiation-defect-a-pilot-study
#8
Manish Jain, Amanpreet Kaur Kalsi, Amita Srivastava, Yogendra Kumar Gupta, Ashutosh Halder
INTRODUCTION: Spermiation is a process of releasing sperm into the lumen of seminiferous tubules. Failure in releasing sperm into the lumen is designated as spermiation defect. Spermiation defect cases present as oligo-azoospermia or azoospermia despite normal gonadotropins and testicular histology/cytology. Human spermiation defect never got attention to investigate infertility practice. Most of the information on spermiation defect, so far is from animal experiments. We assume some cases of non-obstructive azoospermia with normal gonadotropins and testicular histology/cytology could be due to spermiation defect...
December 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28195228/ejaculation-and-sperm-characteristics-in-men-with-cauda-equina-and-conus-medullaris-syndromes
#9
N Hadiji, R Mieusset, J G Previnaire, E Castel-Lacanal, J M Soler
STUDY DESIGN: Retrospective. OBJECTIVES: The objective of the study was to describe the type of ejaculation in patients with cauda equina (CE) and conus medullaris (CM) lesions, and to analyse sperm quality. SETTING: France. METHODS: One hundred sixty-six patients with CE and CM lesions were included. Diagnosis was based on clinical (no motor responses, sensation or sacral reflexes) and urodynamic assessments (no detrusor activity)...
February 14, 2017: Spinal Cord
https://www.readbyqxmd.com/read/28193304/physiological-response-and-semen-quality-of-rabbit-bucks-supplemented-with-moringa-leaves-ethanolic-extract-during-summer-season
#10
N I El-Desoky, N M Hashem, A Elkomy, Z R Abo-Elezz
Exposure of rabbit bucks to summer heat stress reduces their homeostasis and semen quality leading to a temporal subfertility. The potentiality of ethanolic extract of Moringa oleifera leaves (M. oleifera ethanolic extract (MLEE)) to reduce negative impacts of heat stress on physiological and semen quality traits was investigated. A total of 28 adult V-line rabbit bucks were randomly distributed among four experimental groups of seven rabbits each. The first group received water (placebo) and served as a control (M0)...
February 14, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28187743/the-influence-of-macro-and-microelements-in-seminal-plasma-on-diluted-boar-sperm-quality
#11
Maja Zakošek Pipan, Janko Mrkun, Breda Jakovac Strajn, Katarina Pavšič Vrtač, Janko Kos, Anja Pišlar, Petra Zrimšek
BACKGROUND: Growing evidence indicates that macro- and microelements in the seminal plasma of humans and various domestic animals are of great importance due to their roles in sperm metabolism, function, survival and oxidative stress. In the present study, we therefore determined the concentrations of macro- and microelements in fresh boar seminal plasma and their relation to sperm quality parameters after 3 days of liquid storage was assessed. Twenty ejaculates from eight boars were collected, and semen volume, concentration, sperm motility, morphology, tail membrane integrity, plasma membrane permeability, mitochondrial membrane potential and DNA fragmentation were determined on the day of collection (day 0) and day 3 (72 h) of storage at 15-17 °C...
February 10, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28187504/extreme-spermatogenesis-failure-andrological-phenotype-and-intracytoplasmic-sperm-injection-outcomes
#12
P Plouvier, A-L Barbotin, F Boitrelle, D Dewailly, V Mitchell, J-M Rigot, V Lefebvre-Khalil, G Robin
Patients with very low sperm count through direct sperm examination can exhibit extreme oligozoospermia or cryptozoospermia (after centrifugation). The management of these patients is a real challenge for both clinicians and biologists. In this retrospective and comparative cohort study, we compared the andrological phenotype of patients with extreme alterations of spermatogenesis and assessed whether the origin of spermatozoa (testicular or ejaculate) had any influence on intracytoplasmic sperm injection (ICSI) outcomes...
February 10, 2017: Andrology
https://www.readbyqxmd.com/read/28185121/the-association-between-coenzyme-q10-concentrations-in-follicular-fluid-with-embryo-morphokinetics-and-pregnancy-rate-in-assisted-reproductive-techniques
#13
Süleyman Akarsu, Funda Gode, Ahmet Zeki Isik, Zeliha Günnur Dikmen, Mustafa Agah Tekindağ
PURPOSE: This study seeks to evaluate the association between follicular fluid (FF) coenzyme Q10 (CoQ10) levels, embryo morphokinetics, and pregnancy rate. METHODS: Sixty infertile patients who underwent intracytoplasmic sperm injection (ICSI) cycles were included in the study. For each patient, CoQ10 level of the follicular fluid was measured by high-performance liquid chromatography system. After the ICSI of each oocyte, the relationship between the level of CoQ10 content of each follicular fluid, the subsequent embryo quality, and embryo morphokinetics was investigated...
February 9, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28181402/allethrin-toxicity-causes-reproductive-dysfunction-in-male-rats
#14
Golla Madhubabu, Suresh Yenugu
Pyrethroids are widely used for domestic and agricultural purposes and their use is increasing, especially in developing countries. Uncontrolled use of these insecticides resulted in their entry into the food chain thereby causing toxicity to different organ systems. Allethrin is one of the widely used pyrethroids, but its toxicological effects are underreported when compared to other pyrethroids. Further, its effects on the male reproductive tract remain uncharacterized. In this study, its toxicity on the male reproductive tract was evaluated by administering 25-150 mg/kg body weight allethrin to adult rats for 60 days...
February 9, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28178360/melatonin-protects-oocyte-quality-from-bisphenol-a-induced-deterioration-in-the-mouse
#15
Mianqun Zhang, Xiaoxin Dai, Yajuan Lu, Yilong Miao, Changyin Zhou, Zhaokang Cui, Honglin Liu, Bo Xiong
BPA (Bisphenol A) has been reported to adversely affect the mammalian reproductive system in both sexes. However, the underlying mechanisms regarding how BPA disrupts the mammalian oocyte quality and how to prevent it have not been fully defined. Here, we document that BPA weakens oocyte quality by impairing both oocyte meiotic maturation and fertilization ability. We find that oral administration of BPA (100 μg/kg body weight per day for 7 days) compromises the first polar body extrusion (78.0% VS 57.0%, p < 0...
February 8, 2017: Journal of Pineal Research
https://www.readbyqxmd.com/read/28176384/the-effect-of-some-cryoprotectants-on-dromedary-camel-frozen-thawed-semen
#16
D A El-Badry, R H Mohamed, H A El-Metwally, T R Abo Al-Naga
The cryopreserved camel semen is often associated with poor quality and fertility. This study aimed to improve the dromedary frozen semen quality by comparing the efficiency of four cryoprotectant agents (CPAs) on sperm freezability. Semen samples were collected from seven male Maghrabi camels, diluted with Shotor diluent supplemented with glycerol (Sh-G), dimethyl formamide (DMF, Sh-DF), dimethyl sulfoxide (DMSO, Sh-DS) or ethylene glycol (EG, Sh-EG), all at 6% final concentration, and the samples were subjected to cryopreservation...
February 7, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28176378/effects-of-supplemental-conjugated-linoleic-acids-cla-on-fresh-and-post-thaw-sperm-quality-of-holstein-bulls
#17
R Karimi, A Towhidi, S Zeinoaldini, K Rezayazdi, M Mousavi, H Safari, F Martinez-Pastor
This study was designed to investigate the effects of feeding-protected conjugated linoleic acid (CLA) on the semen production and sperm freezability in Holstein bulls. Twelve bulls were randomly assigned to two groups (n = 6 per group). Bulls received the normal diet (control group) or the normal diet top-dressed with 50 g of CLA (treated group) for 10 weeks. The control group received 40 g/day calcium soap of fatty acid. Fresh and post-thaw semen quality was assessed on ejaculates collected at the 0, 4, 6, 8 and 10 week of supplementation...
February 7, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28171687/seminal-coagulation-and-sperm-quality-in-different-social-contexts-in-captive-tufted-capuchin-monkeys-sapajus-apella
#18
Julianne S Lima, Danuza L Leão, Karol G Oliveira, Adriel B Brito, Wlaisa V Sampaio, Regiane R Santos, Helder L Queiroz, Sheyla F Domingues
In the present study, we aimed to assess the influence of different social contexts on the seminal coagulation and sperm quality in captive tufted capuchin monkeys. For this, males were housed either individually, in mixed-sex groups (with females), or in male-only groups. Monkeys were housed in cages and each cage type (i.e., individual or group cage) was placed in a different room. Forty-one males were subjected to semen collection by rectal electroejaculation. The degree of seminal coagulation was determined on a scale of I-IV...
February 7, 2017: American Journal of Primatology
https://www.readbyqxmd.com/read/28166993/resveratrol-and-epigallocatechin-3-gallate-addition-to-thawed-boar-sperm-improves-in%C3%A2-vitro-fertilization
#19
B Gadani, D Bucci, M Spinaci, C Tamanini, G Galeati
Thawing is one of the most delicate process after semen cryopreservation as spermatozoa pass from a dormant metabolic stage to a sudden awakening in cellular metabolism. The rapid oxygen utilization leads to an overproduction of reactive oxygen species that can damage sperm cells, thus causing a significant decrease of fertilizing potential of frozen-thawed spermatozoa. Resveratrol (Res) is a natural grape-derived phytoalexin and Epigallocatechin-3-gallate (EGCG) is the major polyphenol in green tea (Camellia sinensis); both molecules are known to possess high levels of antioxidant activity...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28166992/the-effect-of-dietary-supplementation-of-algae-rich-in-docosahexaenoic-acid-on-boar-fertility
#20
E M Murphy, C Stanton, C O ' Brien, C Murphy, S Holden, R P Murphy, P Varley, M P Boland, S Fair
The objective of this study was to assess the effects of dietary supplementation of a commercial algal product rich in docosahexaenoic acid (DHA) on boar fertility as assessed in vitro and in vivo. Boars were fed one of three experimental diets for 19 weeks: (i) Control (Ctl) diet (n = 31), (ii) Ctl diet plus 75g All-G-Rich per day (n = 31) or (iii) Ctl diet plus 150g All-G-Rich per day (n = 30). Parameters assessed were (i) raw semen quality; volume, sperm concentration, total motility and morphology (ii) liquid semen quality; progressive motility, viability, hypotonic resistance and acrosomal integrity (iii) frozen-thawed semen quality; motility, thermal stress, viability, membrane fluidity and mitochondrial activity (iv) sperm and seminal plasma (SP) fatty acid composition (FAC) (v) total antioxidant capacity (TAC) of SP and (vi) farrowing rates and litter sizes of sows (n = 1158) inseminated with liquid semen...
March 1, 2017: Theriogenology
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