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https://www.readbyqxmd.com/read/28724183/physiological-intra-cytoplasmic-sperm-injection-picsi-outcomes-after-oral-pretreatment-and-semen-incubation-with-myo-inositol-in-oligoasthenoteratozoospermic-men-results-from-a-prospective-randomized-controlled-trial
#1
T Korosi, C Barta, K Rokob, T Torok
OBJECTIVE: The aim of the current study was to evaluate the effect of an oral pretreatment with a mix of myo-inositol (Myo-Ins), folic acid, vitamin E, L-carnitine, L-arginine and selenium (Folandrol, Exeltis, Hungary) and subsequent direct Myo-Ins incubation of spermatozoa before Physiological Intra-Cytoplasmic Sperm Injection (PICSI) procedures in infertile couples due to oligoasthenoteratozoospermia with previous failed PICSI procedures. PATIENTS AND METHODS: We performed a prospective, randomized controlled trial at the Assisted Reproduction Unit of the Kaáli Institute (Győr, Hungary)...
June 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28718081/blastocyst-utilization-rates-after-continuous-culture-in-two-commercial-single-step-media-a-prospective-randomized-study-with-sibling-oocytes
#2
Ioannis A Sfontouris, Efstratios M Kolibianakis, George T Lainas, Christos A Venetis, George K Petsas, Basil C Tarlatzis, Tryfon G Lainas
PURPOSE: The aim of this study is to determine whether blastocyst utilization rates are different after continuous culture in two different commercial single-step media. METHODS: This is a paired randomized controlled trial with sibling oocytes conducted in infertility patients, aged ≤40 years with ≥10 oocytes retrieved assigned to blastocyst culture and transfer. Retrieved oocytes were randomly allocated to continuous culture in either Sage one-step medium (Origio) or Continuous Single Culture (CSC) medium (Irvine Scientific) without medium renewal up to day 5 post oocyte retrieval...
July 17, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28717267/semen-culture-and-the-assessment-of-genitourinary-tract-infections
#3
REVIEW
Michael Solomon, Ralf Henkel
The male factor contributes approximately 50% to infertility-related cases in couples with an estimated 12%-35% of these cases attributable to male genital tract infections. Depending on the nature of the infection, testicular sperm production, sperm transport, and sperm function can be compromised. Yet, infections are potentially treatable causes of infertility. Male genital tract infections are increasingly difficult to detect. Moreover, they often remain asymptomatic ("silent") with the result that they are then passed on to the relevant sexual partner leading to fertilization and pregnancy failure as well as illness of the offspring...
July 2017: Indian Journal of Urology: IJU: Journal of the Urological Society of India
https://www.readbyqxmd.com/read/28716598/effect-of-lecithin-nanoliposome-or-soybean-lecithin-supplemented-by-pomegranate-extract-on-post-thaw-flow-cytometric-microscopic-and-oxidative-parameters-in-ram-semen
#4
Mahdieh Mehdipour, Hossein Daghigh Kia, Maryam Nazari, Abouzar Najafi
This investigation was carried out to study the effect of soybean lecithin 1.5% (wt/vol) (0, 2.5, 5 and 7.5 mg l(-1) pomegranate extract (PE)) or PE-loaded lecithin nanoliposome (0, 2.5, 5 and 7.5 mg l(-1)) to Tris-based extender. Sperm motility (CASA), viability, membrane integrity (HOS test), abnormalities, mitochondrial activity, apoptosis status, lipid peroxidation, total antioxidant capacity (TAC)) and antioxidant activities (GPX, SOD) were investigated following freeze-thawing. No significant differences (P < 0...
July 14, 2017: Cryobiology
https://www.readbyqxmd.com/read/28708514/feeding-rosemary-leaves-powder-ameliorates-rooster-age-related-subfertility
#5
Seyyed Mohsen Borghei-Rad, Saeed Zeinoaldini, Mahdi Zhandi, Hossein Moravej, Mahdi Ansari
Having a high proportion of polyunsaturated fatty acids avian spermatozoa predispose to lipoperoxidation which results in fertility reduction. In the current study, rosemary leaves powder (RLP) was fed to senescent breeder roosters to improve their reproductive performance. Twenty four 70-week-old roosters were randomly divided into four groups and received following treatments including 0 (RLP-0), 2.5 (RLP-2.5), 5 (RLP-5) or 7.5 (RLP-7.5) g of RLP/kg of diet for eight consecutive weeks. Semen characteristics were evaluated weekly...
June 16, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708505/seasonal-changes-of-dna-fragmentation-and-quality-of-raw-and-cold-stored-stallion-spermatozoa
#6
L Wach-Gygax, D Burger, E Malama, H Bollwein, A Fleisch, E Jeannerat, S Thomas, G Schuler, F Janett
In this study annual fluctuations of DNA fragmentation and quality of cold-stored equine sperm were evaluated. Ejaculates were collected weekly during one year from 15 stallions. Ejaculate volume, sperm concentration and total sperm count were determined and semen was then extended and cold-stored for 48 h. Sperm motility was evaluated by CASA before and after 24 as well as 48 h of cold storage. In addition, the percentages of sperm with intact plasma membrane and acrosome (PMAI %) and with low intracellular Ca(2+) level were determined in cold-stored semen (24 h, 48 h)...
May 30, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708497/freezing-dog-semen-using%C3%A2-80%C3%A2-%C3%A2-c-ultra-freezer-sperm-function-and-in%C3%A2-vivo-fertility
#7
F Pezo, C Cheuquemán, P Salinas, J Risopatrón
Long term storage of canine frozen semen is conventionally performed in liquid nitrogen (LN2). However, previous works in freezing canine semen using a -80 °C ultra-freezer (-80°C-UF) showed no differences on sperm quality after thawing. The main objective of this study was to compare the effects of the freezing techniques using LN2 or -80°C-UF on sperm function and in vivo fertility of frozen-thawed dog semen. The sperm-rich fraction of the ejaculate was collected separately from five Chihuahua breed, and each one divided into two aliquots, and frozen and stored in LN2 or -80°C-UF...
May 7, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708490/effect-of-season-and-age-on-indian-red-jungle-fowl-gallus-gallus-murghi-semen-characteristics-a-4-year-retrospective-study
#8
B A Rakha, M S Ansari, S Akhter, E Blesbois
The reproductive potential of the adult males is expected to vary with age/season and largely differ not only in closely related avian species but even in subspecies, breeds and/or strains of the same species. Thus, it is pre-requisite to have knowledge of seminal parameters to achieve maximum production potential of at-risk species for ex situ in vitro conservation programs. A 4-year study was designed to evaluate the effect of age and season (spring, summer, autumn and winter) on semen characteristics of Indian red jungle fowl (Gallus gallus murghi) in a retrospective manner...
May 24, 2017: Theriogenology
https://www.readbyqxmd.com/read/28706397/assessment-of-sperm-deoxyribose-nucleic-acid-fragmentation-using-sperm-chromatin-dispersion-assay
#9
Harsha Pratap, Suma Yekappa Hottigoudar, Kubera Siddappa Nichanahalli, Parkash Chand
The integrity of sperm deoxyribose nucleic acid (DNA) is one of the determinants that ensure normal fertilization, implantation, pregnancy, and the generation of a healthy progeny. Assessment of sperm DNA fragmentation has gained importance as a tool to provide significant information regarding sperm quality, and it can independently predict sperm fertilizing potential. The sperm chromatin dispersion (SCD) assay is one of the available techniques to detect sperm DNA damage with results comparable to the gold standard - Sperm Chromatin Structure Assay...
April 2017: Journal of Pharmacology & Pharmacotherapeutics
https://www.readbyqxmd.com/read/28705580/relationship-between-serum-dioxin-like-polychlorinated-biphenyls-and-post-testicular-maturation-in-human-sperm
#10
Raiza Paul, Julia Moltó, Nuria Ortuño, Alejandro Romero, Carlos Bezos, Jon Aizpurúa, Mª José Gómez-Torres
The relationship between dioxin-like polychlorinated biphenyl (DL-PCB) levels in serum and semen parameters were investigated. Our case-control included two groups of patients. Total concentrations of PCBs were significantly higher in the low semen quality (n=24) than in the normal semen quality (n=26) group. A significant negative correlation was found between PCB 126 and viability in men with low semen quality, while PCBs 77 and 81 were positively correlated with morphology, and PCB 118, mono-ortho and total DL-PCBs were positively correlated with volume...
July 10, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28703337/paternal-age-as-an-independent-factor-does-not-affect-embryo-quality-and-pregnancy-outcomes-of-testicular-sperm-extraction-intracytoplasmic-sperm-injection-in-azoospermia
#11
Y S Park, S H Lee, C K Lim, H W Choi, J H An, C W Park, H S Lee, J S Lee, J T Seo
This study was performed to evaluate the independent influence of paternal age affecting embryo development and pregnancy using testicular sperm extraction (TESE)-intracytoplasmic sperm injection (ICSI) in obstructive azoospermia (OA) and nonobstructive azoospermia (NOA). Paternal patients were divided into the following groups: ≤30 years, 31-35 years, 36-40 years, 41-45 years and ≥46 years. There were no differences in the rates of fertilisation or embryo quality according to paternal and maternal age...
July 13, 2017: Andrologia
https://www.readbyqxmd.com/read/28702741/massive-weight-loss-obtained-by-bariatric-surgery-affects-semen-quality-in-morbid-male-obesity-a-preliminary-prospective-double-armed-study
#12
Jinous Samavat, Giulia Cantini, Francesco Lotti, Alessandra Di Franco, Lara Tamburrino, Selene Degl'Innocenti, Elisa Maseroli, Erminio Filimberti, Enrico Facchiano, Marcello Lucchese, Monica Muratori, Gianni Forti, Elisabetta Baldi, Mario Maggi, Michaela Luconi
OBJECTIVES: The aim of this study is to evaluate the effect of massive weight loss on the seminal parameters at 6 months from bariatric surgery. DESIGN: Two-armed prospective study performed in 31 morbidly obese men, undergoing laparoscopic roux-en-Y-gastric bypass (n = 23) or non-operated (n = 8), assessing sex hormones, conventional (sperm motility, morphology, number, semen volume), and non-conventional (DNA fragmentation and seminal interleukin-8), semen parameters, at baseline and after 6 months from surgery or patients' recruitment...
July 12, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/28700645/overweight-in-young-males-reduce-fertility-in-rabbit-model
#13
Francisco Marco-Jiménez, José Salvador Vicente
Semen quality has certainly declined over the past few decades, possibly owing to modern lifestyle factors. In this sense, the role of overweight and obesity in the development of subfertility in males has generated a considerable amount of interest in recent years. However, there is no consensus on whether overweight or obesity impaired sperm quality. Thus, based on the ongoing debate about risk factors for subfertility associated with overweight and obesity in men, this study was designed to investigate the effect of overweight on sperm quality parameters and fertility success in randomized controlled trial in a rabbit model...
2017: PloS One
https://www.readbyqxmd.com/read/28699207/supplemental-effect-of-different-levels-of-taurine-in-modena-on-boar-semen-quality-during-liquid-preservation-at-17%C3%A2-c
#14
Hao Li, Xiao-Gang Zhang, Qian Fang, Qi Liu, Ren-Rang Du, Gong-She Yang, Li-Qiang Wang, Jian-Hong Hu
Peroxidation damage induces sublethal injury to boar sperm during the storage process. Taurine has already been demonstrated to protect cells effectively from oxidant-induced injury. This study was aimed to evaluate the effect of different concentrations of taurine (0.5, 1, 5 and 10 mmol/L) in Modena diluent on boar sperm quality during liquid storage at 17°C. Ejaculates from sexually mature Duroc pigs were collected, pooled and preserved in the Modena containing different concentrations of taurine. Sperm motility, plasma membrane integrity, acrosome integrity, total antioxidative capacity (T-AOC) activity and malondialdehyde content (MDA) were examined every 24 h...
July 12, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28697988/the-addition-of-reduced-glutathione-to-cryopreservation-media-induces-changes-in-the-structure-of-motile-subpopulations-of-frozen-thawed-boar-sperm
#15
Efrén Estrada, Maria M Rivera Del Álamo, Joan E Rodríguez-Gil, Marc Yeste
Adding cryopreservation media with reduced glutathione (GSH) has previously been shown to maintain the motility, membrane integrity and fertilizing ability of frozen-thawed boar sperm, although the effects of GSH on good (GFE) and poor freezability (PFE) ejaculates rely upon the intrinsic ejaculate freezability. The resilience to withstand freeze-thawing procedures has previously been related to the existence of a specific distribution of motile sperm subpopulations, which differs between GFE and PFE. Thus, the main aim of this study was to determine whether the addition of GSH to freezing media has any impact on the distribution of motile sperm subpopulations in GFE and PFE...
July 8, 2017: Cryobiology
https://www.readbyqxmd.com/read/28695635/taurine-does-not-improve-the-quality-of-short-term-stored-rabbit-spermatozoa-in-vitro
#16
D Paál, F Strejček, E Tvrdá, J Vašíček, A Baláži, P Chrenek, P Massányi
This study examines the impact of taurine on the viability, morphology and acrosome integrity of rabbit spermatozoa in vitro. Semen samples, obtained from four to five sexually mature and healthy New Zealand White rabbits, were pooled in heterospermic semen sample. This was divided and treated with taurine in a concentration of 0 (control), 1.5, 7, 12.5, 50 mM to a final concentration of 10(8)  spermatozoa/ml. The samples were then incubated at 37°C for 4 hr. A combination of fluorescent probes SYBR-14/propidium iodide/PNA-Alexa Fluor 647 was used to assess spermatozoa viability and acrosome integrity on a flow cytometer...
July 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28695606/orally-administered-chrysin-improves-post-thawed-sperm-quality-and-fertility-of-rooster
#17
M Zhandi, M Ansari, P Roknabadi, A Zare Shahneh, M Sharafi
Chrysin is a bioflavonoid compound found in passion flower, chamomile, propolis and honey at high levels. Post-thawed sperm quality and fertility of Chrysin-fed roosters were assessed in this study. Twenty 40-week-old male broiler breeders were randomly divided into four groups and fed basal diet supplemented with different levels of Chrysin including 0 (Ch-0), 25 (Ch-25), 50 (Ch-50) or 75 (Ch-75) mg/day for 12 consecutive weeks. Semen samples were weekly collected from 6th to 9th week of experiment to evaluate some sperm quality parameters including total and progressive motility, plasma membrane integrity and functionality (in fresh and post-thawed samples) and mitochondrial activity (only in post-thawed samples)...
July 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28694462/transgenerational-inheritance-of-susceptibility-to-diabetes-induced-male-subfertility
#18
Gabriela Pavlinkova, Hasmik Margaryan, Eva Zatecka, Eliska Valaskova, Fatima Elzeinova, Alena Kubatova, Romana Bohuslavova, Jana Peknicova
Male infertility is a worldwide problem associated with genetic background, environmental factors, and diseases. One of the suspected contributing factors to male infertility is diabetes mellitus. We investigated the molecular and morphological changes in sperms and testicular tissue of diabetic males. The study was performed in streptozotocin-induced type 1 diabetes mouse model. Diabetes decreased sperm concentration and viability and increased sperm apoptosis. Changes in protamine 1/protamine 2 ratio indicated reduced sperm quality...
July 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28692609/human-semen-quality-sperm-dna-damage-and-the-level-of-reproductive-hormones-in-relation-to-urinary-concentrations-of-parabens
#19
Joanna Jurewicz, Michał Radwan, Bartosz Wielgomas, Emila Dziewirska, Anetta Karwacka, Anna Klimowska, Paweł Kałużny, Paweł Radwan, Michał Bochenek, Wojciech Hanke
OBJECTIVE: The aim of this study was to evaluate the association between environmental exposure to parabens and semen quality parameters [main semen parameters, computer-aided semen analysis (CASA parameters], sperm chromatin structure, and the level of reproductive hormones in men [follicle-stimulating hormone (FSH), testosterone, estradiol]. METHODS: Urine samples collected from 315 men who attended the infertility clinic for diagnostic purposes with normal semen concentration of 15 to 300 mln/mL were analyzed for five parabens concentrations using a validated gas chromatography ion-tap mass spectrometry method...
July 7, 2017: Journal of Occupational and Environmental Medicine
https://www.readbyqxmd.com/read/28691350/serum-testosterone-sperm-quality-cytological-physicochemical-and-biochemical-characteristics-of-the-prostatic-fraction-of-dogs-with-prostatomegaly
#20
A Aquino-Cortez, B Q Pinheiro, Hvr Silva, Dbc Lima, Tfp Silva, M B Souza, D A Viana, Faf Xavier Júnior, Jsam Evangelista, F Z Brandão, Ldm Silva
Prostatomegaly is a common finding in older non-neutered dogs. This study compared the serum testosterone, sperm quality and characteristics of the prostatic fraction between healthy dogs and dogs with prostatomegaly. Blood samples of ten dogs (five dogs from each group) were taken for serum testosterone measurement. Sperm motility, vigour, concentration, viability, membrane functionality and morphology were analysed in sperm-rich fraction. Osmolality, pH, cell types, and albumin, haemoglobin, acid phosphatase, alkaline phosphatase, glucose, triglycerides, cholesterol, calcium, phosphorus, magnesium and chloride were analysed in prostatic fraction...
July 9, 2017: Reproduction in Domestic Animals, Zuchthygiene
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