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https://www.readbyqxmd.com/read/28527403/interplay-between-bacillus-subtilis-recd2-and-the-recg-or-ruvab-helicase-in-recombinational-repair
#1
Rubén Torres, Hector Romero, Violeta Rodríguez-Cerrato, Juan C Alonso
Bacillus subtilis AddAB, RecS, RecQ, PcrA, HelD, DinG, RecG, RuvAB, PriA and RecD2 are genuine recombinational repair enzymes, but the biological role of RecD2 is poorly defined. A ΔrecD2 mutation sensitizes cells to DNA-damaging agents that stall or collapse replication forks. We found that this ΔrecD2 mutation impaired growth, and that a mutation in the pcrA gene (pcrA596) relieved this phenotype. The ΔrecD2 mutation was not epistatic to ΔaddAB, ΔrecQ, ΔrecS, ΔhelD, pcrA596 and ΔdinG, but epistatic to recA...
May 12, 2017: DNA Repair
https://www.readbyqxmd.com/read/28511132/activity-and-in-vivo-dynamics-of-bacillus-subtilis-disa-are-affected-by-rada-sms-and-by-holliday-junction-processing-proteins
#2
Carolina Gándara, Daniella K C de Lucena, Rubén Torres, Ester Serrano, Stephan Altenburger, Peter L Graumann, Juan C Alonso
Bacillus subtilis c-di-AMP synthase DisA and RecA-related RadA/Sms are involved in the repair of DNA damage in exponentially growing cells. We provide genetic evidence that DisA or RadA/Sms is epistatic to the branch migration translocase (BMT) RecG and the Holliday junction (HJ) resolvase RecU in response to DNA damage. We provide genetic evidence damage. Functional DisA-YFP formed dynamic foci in exponentially growing cells, which moved through the nucleoids at a speed compatible with a DNA-scanning mode...
May 5, 2017: DNA Repair
https://www.readbyqxmd.com/read/28509381/topography-guided-control-of-local-migratory-behaviors-and-protein-expression-of-cancer-cells
#3
Jung-Youn Shin, Hong Nam Kim, Suk Ho Bhang, Jeong-Kee Yoon, Kahp-Yang Suh, Noo Li Jeon, Byung-Soo Kim
In vivo cancer cell migration and invasion are directed by biophysical guidance mechanisms such as pre-existing microtracks and basement membrane extracellular matrices. Here, this paper reports the correlation of the local migratory behavior of cancer cells and the biochemical signal expression using the topography that can guide or inhibit cell behaviors. To this end, the local apparent migration and the protein expression level are investigated with respect to the topographical feature size (flat, nanoline, and microline) and orientation (microline, microconcentric, and microradial) with the collectively migrating (A431) and individually migrating (MDA-MB-231 and U-87-MG) cancer cells...
May 16, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28410532/role-of-p16-ink4a-and-bmi-1-in-oxidative-stress-induced-premature-senescence-in-human-dental-pulp-stem-cells
#4
Cristina Mas-Bargues, José Viña-Almunia, Marta Inglés, Jorge Sanz-Ros, Juan Gambini, José Santiago Ibáñez-Cabellos, José Luis García-Giménez, José Viña, Consuelo Borrás
Human dental pulp stem cells (hDPSCs) are a source for cell therapy. Before implantation, an in vitro expansion step is necessary, with the inconvenience that hDPSCs undergo senescence following a certain number of passages, loosing their stemness properties. Long-term in vitro culture of hDPSCs at 21% (ambient oxygen tension) compared with 3-6% oxygen tension (physiological oxygen tension) caused an oxidative stress-related premature senescence, as evidenced by increased β-galactosidase activity and increased lysil oxidase expression, which is mediated by p16(INK4a) pathway...
April 7, 2017: Redox Biology
https://www.readbyqxmd.com/read/27903910/structural-insights-into-dynamics-of-recu-hj-complex-formation-elucidates-key-role-of-ntr-and-stalk-region-toward-formation-of-reactive-state
#5
Sagar Khavnekar, Sarath Chandra Dantu, Svetlana Sedelnikova, Sylvia Ayora, John Rafferty, Avinash Kale
Holliday junction (HJ) resolving enzyme RecU is involved in DNA repair and recombination. We have determined the crystal structure of inactive mutant (D88N) of RecU from Bacillus subtilis in complex with a 12 base palindromic DNA fragment at a resolution of 3.2 Å. This structure shows the stalk region and the essential N-terminal region (NTR) previously unseen in our DNA unbound structure. The flexible nature of the NTR in solution was confirmed using SAXS. Thermofluor studies performed to assess the stability of RecU in complex with the arms of an HJ indicate that it confers stability...
January 25, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/27799325/muts2-promotes-homologous-recombination-in-bacillus-subtilis
#6
Peter E Burby, Lyle A Simmons
Bacterial MutS proteins are subdivided into two families, MutS1 and MutS2. MutS1 family members recognize DNA replication errors during their participation in the well-characterized mismatch repair (MMR) pathway. In contrast to the well-described function of MutS1, the function of MutS2 in bacteria has remained less clear. In Helicobacter pylori and Thermus thermophilus, MutS2 has been shown to suppress homologous recombination. The role of MutS2 is unknown in the Gram-positive bacterium Bacillus subtilis In this work, we investigated the contribution of MutS2 to maintaining genome integrity in B...
January 15, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/27268429/casein-kinase1-like-protein2-regulates-actin-filament-stability-and-stomatal-closure-via-phosphorylation-of-actin-depolymerizing-factor
#7
Shuangshuang Zhao, Yuxiang Jiang, Yang Zhao, Shanjin Huang, Ming Yuan, Yanxiu Zhao, Yan Guo
The opening and closing of stomata are crucial for plant photosynthesis and transpiration. Actin filaments undergo dynamic reorganization during stomatal closure, but the underlying mechanism for this cytoskeletal reorganization remains largely unclear. In this study, we identified and characterized Arabidopsis thaliana casein kinase 1-like protein 2 (CKL2), which responds to abscisic acid (ABA) treatment and participates in ABA- and drought-induced stomatal closure. Although CKL2 does not bind to actin filaments directly and has no effect on actin assembly in vitro, it colocalizes with and stabilizes actin filaments in guard cells...
June 2016: Plant Cell
https://www.readbyqxmd.com/read/27131283/tuning-of-the-stability-and-energy-levels-of-singlet-exciton-fission-relevant-excited-states-of-pentacenes-by-site-specific-substitution
#8
Li Shen, Yuhan Chen, Xiyou Li, Chungang Li
Pentacene crystals or oligomers undergo efficient singlet exciton fission (SF) after photo-excitation, which is expected to be useful in overcoming the Shockley-Queisser theoretical limit of solar cells. However, pentacenes are extremely unstable in air due to oxidation by oxygen. In this work, we designed a group of pentacene compounds with different substituents at different positions. The energy levels of HOMO and LUMO, which are believed to be closely related to the stability of pentacene, were calculated...
May 2016: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/27085264/alterations-in-intraocular-cytokine-levels-following-intravitreal-ranibizumab
#9
Farzin Forooghian, Peter J Kertes, Kenneth T Eng, David A Albiani, Andrew W Kirker, Andrew B Merkur, Nader Fallah, Sijia Cao, Jing Cui, Chris Or, Joanne A Matsubara
OBJECTIVE: Our previous work has shown that, after intravitreal bevacizumab (IVB) administration, decreases in the levels of vascular endothelial growth factor (VEGF)-A and placental growth factor (PlGF), along with increases in the levels of interleukin (IL)-8 and transforming growth factor (TGF)-β2, can be observed. It is not yet known if similar changes occur after intravitreal ranibizumab (IVR). The purpose of this study was to examine intraocular cytokine changes after IVR. DESIGN: Prospective clinical study...
April 2016: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
https://www.readbyqxmd.com/read/27047566/comparison-between-intraperitoneal-and-intravenous-lidocaine-for-postoperative-analgesia-after-elective-abdominal-hysterectomy-a-double-blind-placebo-controlled-study
#10
Saghar Samimi, Arman Taheri, Fatemeh Davari Tanha
OBJECTIVE: To compare the efficacy of intravenous and intraperitoneal injection of lidocaine and normal saline in relieving postoperative pain after elective abdominal hysterectomy. MATERIALS AND METHODS: For this double-blind randomized controlled study 109 patients undergoing elective abdominal hysterectomy were randomly allocated to three groups :1) IV group (intravenous injection group) received intravenous lidocaine %2 bolus 1.5mg/kg 30 min before incision and then a continuous lidocaine infusion of 2mg/kg and before the wound closure an intraperitoneal injection of N/S , 2) IP group (intraperitoneal group) received intravenous N/S and intraperitoneal lidocaine 3mg/kg , 3) P group (placebo, N/S) received both intravenous and intraperitoneal N/S...
November 2015: Journal of Family & Reproductive Health
https://www.readbyqxmd.com/read/26877477/p-171-pregnancy-induced-hypertension-in-patients-with-mitral-stenosis
#11
N Benatta, L Hamou, D D Batouche, T Merzouk, M Benbouabdellah
OBJECTIFS: La prise en charge du rétrécissement mitral (RM) associé à l'hypertension gravidique continue à poser un défi au médecin particulièrement dans les pays en voie de développement. Le but de cette étude était d'étudier le profil clinique, thérapeutique et évolutif des RM accompagnés hypertension gravidique. MéTHODES: 83 patientes atteintes d'un rétrécissement mitral suivis durant la grossesse dont 7 patientes ont compliqué d'une Hypertension gravidique. RéSULTATS: Le diagnostic de l'Hypertension gravidique a été posé par la MAPA, le RM était serré dans les 7 cas avec une surface mitrale anatomique entre 1 et 1,5cm(2)...
December 2015: Annales de Cardiologie et D'angéiologie
https://www.readbyqxmd.com/read/26877393/p-090-the-autonomic-disorders-and-high-blood-pressure-during-guillain-barre-syndrome-in-children-crossed-university-hospital-center-in-oran
#12
D Batouche, K Elhalimi, N Chaib, S Abassini, Y Touhami, M A Negadi, Z Mentouri
OBJECTIFS: Le syndrome de Guillain-Barré (SGB) est une polyradiculonévrite aiguë dont l'évolution peut nécessiter une prise en charge en réanimation. Les complications dysautonomiques neurovégétatives cardiovasculaires (DNVcv) sont importantes à connaître car elles peuvent être responsables d'un mauvais pronostic. Notre objectif est de déterminer la prévalence et le pronostic de la DNVcv. MéTHODES: Nous avons entamé un travail au sein du Service de Réanimation Pédiatrique du CHU d'Oran durant la période de janvier 2011 à aout 2015...
December 2015: Annales de Cardiologie et D'angéiologie
https://www.readbyqxmd.com/read/26877359/p-058-recovery-of-renal-function-in-malignant-hypertension-patients-on-dialysis
#13
M Khalfaoui, N Mtioui, S S Khayat, M A Zamd, G Medkouri, M Benghanem Gharbi, B Ramdani
OBJECTIFS: L'HTA maligne est une cause rare de micro-angiopathie throm-botique (MAT) de l'adulte. La (MAT) est la lésion commune du syndrome hémolytique et urémique (SHU). Les rémissions spontanées sont possibles chez certains patients qui n'ont reçu qu'un traitement symptomatique à base d'anitihypertenseurs. MéTHODES: Nous rapportons 4 cas de sortie de dialyse chez des malades ayant une HTA maligne. RéSULTATS: Il s'agissait de 3 hommes et d'1 femme âgés en moyenne de 38 ans...
December 2015: Annales de Cardiologie et D'angéiologie
https://www.readbyqxmd.com/read/26877345/co-03-evaluation-of-the-screening-for-myocardial-ischemia-among-hypertensive-women-at-risk-of-cardiovascular-diseases
#14
A Madika, C Mounier-Vehier, F Boudghene, P Delsart, N Kpogbemabou
OBJECTIFS: La maladie coronaire est au premier rang des maladies cardiovas-culaires, qui reste la première cause de mortalité chez la femme. Chez elle, la prévention, le dépistage et les épreuves diagnostiques tendent à être tardifs et moins fréquemment réalisés, et le traitement sous-optimal. Il n'existe pas actuellement de données de la littérature sur les conséquences d'un dépistage ciblé de l'ischémie myocardique chez la femme à risque. La sélection des patientes qui bénéficieront le plus de ce dépistage reste encore à définir...
December 2015: Annales de Cardiologie et D'angéiologie
https://www.readbyqxmd.com/read/26877334/p4-36-home-orthostatic-hypotension-measurement-in-the-elderly-feasability-and-relevance
#15
E Duron, J Vidal, F Roca, H Rananja, C Boully, L Caillard, S Chauvelier, M Seux, A Rigaud, O Hanon
OBJECTIFS: Déterminer la faisabilité et la pertinence de en auto-mesure ou auto-mesure aidée de l'hypotension orthostatique (HO) au domicile chez de sujets âgés ambulatoires. MéTHODES: 185 patients évalués en hôpital de jour (HDJ) d'évaluation gériatrique, âgés de plus de 60 ans, ayant un MMSE>25 ou un aidant naturel pouvant effectuer les mesures et pouvant tenir debout au moins 3 minutes, ont été inclus. L'HO était mesurée à 1 et 3 minutes lors de la consultation Les participants ou leur aidant principal ont reçu une information détaillée sur les modalités de la recherche d'HO à domicile...
December 2015: Annales de Cardiologie et D'angéiologie
https://www.readbyqxmd.com/read/26820318/very-early-initiation-of-chemical-venous-thromboembolism-prophylaxis-after-blunt-solid-organ-injury-is-safe
#16
COMPARATIVE STUDY
Patrick B Murphy, Niroshan Sothilingam, Tanya Charyk Stewart, Brandon Batey, Brad Moffat, Daryl K Gray, Neil G Parry, Kelly N Vogt
BACKGROUND: The optimal timing of initiating low-molecular weight heparin (LMWH) in patients who have undergone nonoperative management (NOM) of blunt solid organ injuries (SOIs) remains controversial. We describe the safety of early initiation of chemical venous thromboembolism (VTE) prophylaxis among patients undergoing NOM of blunt SOIs. METHODS: We retrospectively studied severely injured adults who sustained blunt SOI without significant intracranial hemorrhage and underwent an initial NOM at a Canadian lead trauma hospital between 2010 and 2014...
April 2016: Canadian Journal of Surgery. Journal Canadien de Chirurgie
https://www.readbyqxmd.com/read/26806755/-impact-of-sperm-dna-and-acrosome-integrity-and-acrosome-reaction-rate-on-outcomes-of-rescue-intracytoplasmic-sperm-injection
#17
Yongzhi He, Dawen Li, Junping Cheng, Zhongchao Huo, Hongyi Huang, Xin Xiao
UNLABELLED: Objective To explore the effects of sperm DNA integrity rate, acrosome integrity rate and acrosome reaction rate on the outcomes of rescue intracytoplasmic sperm injection (ICSI). METHODS: This retrospective analysis was conducted among 97 infertile couples receiving rescue ICSI due to failure of in vitro fertilization procedures in our Reproductive Medicine Center. Of these 97 women, 41 had clinical pregnancy and 56 did not, and the effects of sperm DNA integrity rate (estimated by DNA fragmentation index, DFI), acrosome integrity rate and acrosome reaction rate on rescue ICSI outcomes were analyzed...
January 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/26386687/e804-induces-growth-arrest-differentiation-and-apoptosis-of-glioblastoma-cells-by-blocking-stat3-signaling
#18
Yanmin Zhang, Zhaoxiao Du, Zirong Zhuang, Yanjun Wang, Fen Wang, Shuai Liu, Han Wang, Hairong Feng, Haoyuan Li, Liyan Wang, Xianghong Zhang, Aijun Hao
Glioblastoma, the most common type of primary malignant brain tumor, is a devastating disease associated with a median survival of approximately 12 months. Here, we have tested E804, the commercially available indirubin derivatives, against U251 and U87 glioblastoma cells. Treatment with E804 significantly inhibits the growth of human glioblastoma cells lines via induction of differentiation and apoptosis. Differentiation induction is coupled with increased expression of glial fibriliary acidic protein, a marker for mature astrocytes...
November 2015: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/26346199/efficacy-of-acupuncture-versus-combined-oral-contraceptive-pill-in-treatment-of-moderate-to-severe-dysmenorrhea-a-randomized-controlled-trial
#19
Intira Sriprasert, Suparerk Suerungruang, Porntip Athilarp, Anuchart Matanasarawoot, Supanimit Teekachunhatean
This open-label randomized controlled trial was designed to compare the efficacy of acupuncture and combined oral contraceptive (COC) pill in treating moderate-to-severe primary dysmenorrhea. Fifty-two participants were randomly assigned to receive either acupuncture (n = 27) or COC (n = 25) for three menstrual cycles. Mefenamic acid was prescribed as a recue analgesic drug with both groups. The statistical approach used for efficacy and safety assessments was intention-to-treat analysis. By the end of the study, both treatments had resulted in significant improvement over baselines in all outcomes, that is, maximal dysmenorrhea pain scores, days suffering from dysmenorrhea, amount of rescue analgesic used, and quality of life assessed by SF-36 questionnaire...
2015: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/26219080/end-of-life-co-residence-of-older-parents-and-their-sons-in-rural-china
#20
Zhen Cong, Merril Silverstein
This study examined how intergenerational exchanges with sons and daughters predicted older parents' likelihood of co-residing with a son prior to death in a rural area of China's Anhui Province. Our investigation drew on theories of contingent co-residence, modernization, and social exchange, conceptualizing co-residence as having practical and symbolic importance in rural Chinese culture. The sample included 470 older parents, reported as deceased during 2001-2009, and their posthumous informants. We used logistic regression to assess intergenerational support and cohesion as predictors of co-residence with a son just prior to death...
September 2015: Canadian Journal on Aging, la Revue Canadienne du Vieillissement
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