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https://www.readbyqxmd.com/read/29756589/effect-of-combination-therapy-with-neuroprotective-and-vasoprotective-agents-on-cerebral-ischemia
#1
Jiping Yang, Bei Yang, Baoxin Xiu, Jinchong Qi, Huaijun Liu
Because most tested drugs are active against only one of the damaging processes associated with stroke, other mechanisms may cause cellular death. Thus, a combination of protective agents targeting different pathophysiological mechanisms may obtain better effects than a single agent. The major objective of this study was to investigate the effect of combination therapy with vascular endothelial growth factor (VEGF) and nerve growth factor (NGF) after controlled ischemic brain injury in rabbits. METHODS: Animals were randomly assigned to one of the following groups: sham group, saline-treated control group or NGF+VEGF-treated group...
May 2018: Canadian Journal of Neurological Sciences. le Journal Canadien des Sciences Neurologiques
https://www.readbyqxmd.com/read/29710510/long-non-coding-rna-nnt-as1-affects-progression-of-breast-cancer-through-mir-142-3p-zeb1-axis
#2
Yan Li, Min Lv, Ziyan Song, Zhi Lou, Ran Wang, Min Zhuang
Some evidences have been provided to verify the effects of lncRNA NNT-AS1 on cancer progression. However, the crucial impacts of NNT-AS1 on the malignancy of breast cancer have not been elaborated. This study aims to detect the expression pattern and functional effects of NNT-AS1 in breast cancer. qRT-PCR analysis was applied to detect the expression of NNT-AS1 in both BC tissues and matched normal tissues. Loss of function assay was carried out to detect the effects of silenced NNT-AS1 on proliferation, metastasis and EMT process of BC cells...
April 24, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29648978/fgf21-protects-the-blood-brain-barrier-by-upregulating-ppar%C3%AE-via-fgfr1-%C3%AE-klotho-following-traumatic-brain-injury
#3
Jun Chen, Jian Hu, Huan Liu, Ye Xiong, Yuchi Zou, Wenting Huang, Mingjie Shao, Jiamin Wu, Li Yu, Xiaojie Wang, Xue Wang, Li Lin
Blood-brain barrier (BBB) disruption and dysfunction result in brain edema, which is responsible for more than half of all deaths after severe traumatic brain injury (TBI). Fibroblast growth factor 21 (FGF21) has a potential neuroprotective function in the brain. However, the effects and underlying possible mechanism of action on BBB integrity following TBI remain unknown. The purpose of the current study was to determine the effects of FGF21 on BBB protection and TBI treatment. The effects of recombinant human FGF21 (rhFGF21) on BBB integrity and on tight junction and adherens junction proteins were investigated both in a TBI mouse model and an in vitro BBB disruption model established with TNF-α-induced human brain microvascular endothelial cells (HBMECs)...
March 6, 2018: Journal of Neurotrauma
https://www.readbyqxmd.com/read/29538234/intravenous-administration-of-synthetic-platelets-synthoplatetm-in-a-mouse-liver-injury-model-of-uncontrolled-hemorrhage-improves-hemostasis
#4
Mitchell R Dyer, DaShawn Hickman, Norman Luc, Shannon Haldeman, Patricia Loughran, Christa Pawlwoski, Anirban Sen Gupta, Matthew D Neal
BACKGROUND: Clinical resuscitative treatment of traumatic hemorrhage involves transfusion of RBC, platelets and plasma in controlled ratios. However, use of such blood components, especially platelets, present many challenges including availability, portability, contamination risks, and short shelf-life, which limit the use of platelet transfusions outside of large trauma centers such as remote civilian hospitals and austere pre-hospital settings. This has prompted significant research in platelet substitutes that may resolve the above issues while providing platelet-mimetic hemostatic action...
March 12, 2018: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/29247687/genotypic-variations-between-wild-type-and-small-colony-variant-of-staphylococcus-aureus-in-prosthetic-valve-infectious-endocarditis-a-comparative-genomic-and-transcriptomic-analysis
#5
Hongbin Chen, Qi Wang, Yuyao Yin, Shuguang Li, Deng-Ke Niu, Hui Wang
Staphylococcus aureus small colony variants (SCVs) can cause persistent infections. However, the genomes and transcriptomes of S. aureus SCVs remain poorly understood. A pair of isogenic wild-type and SCV methicillin-resistant S. aureus (MRSA) strains (IE1 and IE2, respectively) were isolated from a patient with prosthetic valve infectious endocarditis. The SCV strain IE2 grew more slowly than the wild-type strain, and serum killing and mouse lethality assays revealed that the virulence of SCV strain IE2 was decreased...
December 13, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29244083/nucleomethylin-deficiency-impairs-embryonic-erythropoiesis
#6
Shohei Murakami, Takuma Suzuki, Wataru Yokoyama, Satoko Yagi, Keita Matsumura, Yuka Nakajima, Hideo Harigae, Akiyoshi Fukamizu, Hozumi Motohashi
Nucleomethylin (NML) has been shown to contribute to ribosome formation through regulating transcription and post-transcriptional modification of rRNA. Based on the observation that NML-/- mice are frequently embryonic lethal, we analyzed NML-/- embryos to clarify the role of NML in embryogenesis. We found that NML deficiency leads to lethality at the time point between E10.5 and E12.5. Most of E10.5 NML-/- embryos exhibited growth retardation and/or malformation with marked impairment of erythropoiesis. Consistent with a previous study, the m1A in 28S rRNA was dramatically reduced in NML-/- fetal liver cells...
December 13, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28961460/bacillus-subtilis-disa-helps-to-circumvent-replicative-stress-during-spore-revival
#7
Marina Raguse, Rubén Torres, Elena M Seco, Carolina Gándara, Silvia Ayora, Ralf Moeller, Juan C Alonso
The mechanisms that allow to circumvent replicative stress, and to resume DNA synthesis are poorly understood in Bacillus subtilis. To study the role of the diadenylate cyclase DisA and branch migration translocase (BMT) RadA/Sms in restarting a stalled replication fork, we nicked and broke the circular chromosome of an inert mature haploid spore, damaged the bases, and measured survival of reviving spores. During undisturbed ripening, nicks and breaks should be repaired by pathways that do not invoke long-range end resection or genetic exchange by homologous recombination, after which DNA replication might be initiated...
November 2017: DNA Repair
https://www.readbyqxmd.com/read/28771880/limited-effectiveness-with-a-10-day-bismuth-containing-quadruple-therapy-pylera-%C3%A2-in-third-line-recue-treatment-for-helicobacter-pylori-infection-a-real-life-multicenter-study
#8
Enrique Rodríguez de Santiago, Carlos Martín de Argila de Prados, Hector Miguel Marcos Prieto, Miguel Ãngel Jorge Turrión, Eva Barreiro Alonso, Alvaro Flores de Miguel, Cristobal de la Coba Ortiz, Carlos Rodríguez Escaja, Gustavo Pérez Álvarez, Carlos Ferre Aracil, Lara Aguilera Castro, Ana García García de Paredes, Antonio Rodríguez Pérez, Agustin Albillos Martínez
BACKGROUND: Helicobacter pylori antibiotic resistance is an increasing problem worldwide. Pylera(®) may be an option as salvage therapy. AIM: To assess the effectiveness, safety, and tolerance of Pylera(®) as a third-line in clinical practice. MATERIALS AND METHODS: This was a multicenter, observational, prospective database study in four Spanish hospitals. Consecutive H. pylori-infected individuals treated with Pylera(®) and a proton-pump inhibitor (PPI) were invited to participate if they had failed to respond to PPI-clarithromycin-amoxicillin as first-line and to levofloxacin-amoxicillin-PPI as second-line therapy...
August 3, 2017: Helicobacter
https://www.readbyqxmd.com/read/28532385/erratum-to-the-holliday-junction-resolvase-recu-is-required-for-chromosome-segregation-and-dna-damage-repair-in-staphylococcus-aureus
#9
Ana R Pereira, Patricia Reed, Helena Veiga, Mariana G Pinho
No abstract text is available yet for this article.
May 22, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28527403/interplay-between-bacillus-subtilis-recd2-and-the-recg-or-ruvab-helicase-in-recombinational-repair
#10
Rubén Torres, Hector Romero, Violeta Rodríguez-Cerrato, Juan C Alonso
Bacillus subtilis AddAB, RecS, RecQ, PcrA, HelD, DinG, RecG, RuvAB, PriA and RecD2 are genuine recombinational repair enzymes, but the biological role of RecD2 is poorly defined. A ΔrecD2 mutation sensitizes cells to DNA-damaging agents that stall or collapse replication forks. We found that this ΔrecD2 mutation impaired growth, and that a mutation in the pcrA gene (pcrA596) relieved this phenotype. The ΔrecD2 mutation was not epistatic to ΔaddAB, ΔrecQ, ΔrecS, ΔhelD, pcrA596 and ΔdinG, but epistatic to recA...
July 2017: DNA Repair
https://www.readbyqxmd.com/read/28511132/activity-and-in-vivo-dynamics-of-bacillus-subtilis-disa-are-affected-by-rada-sms-and-by-holliday-junction-processing-proteins
#11
Carolina Gándara, Daniella K C de Lucena, Rubén Torres, Ester Serrano, Stephan Altenburger, Peter L Graumann, Juan C Alonso
Bacillus subtilis c-di-AMP synthase DisA and RecA-related RadA/Sms are involved in the repair of DNA damage in exponentially growing cells. We provide genetic evidence that DisA or RadA/Sms is epistatic to the branch migration translocase (BMT) RecG and the Holliday junction (HJ) resolvase RecU in response to DNA damage. We provide genetic evidence damage. Functional DisA-YFP formed dynamic foci in exponentially growing cells, which moved through the nucleoids at a speed compatible with a DNA-scanning mode...
July 2017: DNA Repair
https://www.readbyqxmd.com/read/28509381/topography-guided-control-of-local-migratory-behaviors-and-protein-expression-of-cancer-cells
#12
Jung-Youn Shin, Hong Nam Kim, Suk Ho Bhang, Jeong-Kee Yoon, Kahp-Yang Suh, Noo Li Jeon, Byung-Soo Kim
In vivo cancer cell migration and invasion are directed by biophysical guidance mechanisms such as pre-existing microtracks and basement membrane extracellular matrices. Here, this paper reports the correlation of the local migratory behavior of cancer cells and the biochemical signal expression using the topography that can guide or inhibit cell behaviors. To this end, the local apparent migration and the protein expression level are investigated with respect to the topographical feature size (flat, nanoline, and microline) and orientation (microline, microconcentric, and microradial) with the collectively migrating (A431) and individually migrating (MDA-MB-231 and U-87-MG) cancer cells...
August 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28410532/role-of-p16-ink4a-and-bmi-1-in-oxidative-stress-induced-premature-senescence-in-human-dental-pulp-stem-cells
#13
Cristina Mas-Bargues, José Viña-Almunia, Marta Inglés, Jorge Sanz-Ros, Juan Gambini, José Santiago Ibáñez-Cabellos, José Luis García-Giménez, José Viña, Consuelo Borrás
Human dental pulp stem cells (hDPSCs) are a source for cell therapy. Before implantation, an in vitro expansion step is necessary, with the inconvenience that hDPSCs undergo senescence following a certain number of passages, loosing their stemness properties. Long-term in vitro culture of hDPSCs at 21% (ambient oxygen tension) compared with 3-6% oxygen tension (physiological oxygen tension) caused an oxidative stress-related premature senescence, as evidenced by increased β-galactosidase activity and increased lysil oxidase expression, which is mediated by p16(INK4a) pathway...
August 2017: Redox Biology
https://www.readbyqxmd.com/read/27903910/structural-insights-into-dynamics-of-recu-hj-complex-formation-elucidates-key-role-of-ntr-and-stalk-region-toward-formation-of-reactive-state
#14
Sagar Khavnekar, Sarath Chandra Dantu, Svetlana Sedelnikova, Sylvia Ayora, John Rafferty, Avinash Kale
Holliday junction (HJ) resolving enzyme RecU is involved in DNA repair and recombination. We have determined the crystal structure of inactive mutant (D88N) of RecU from Bacillus subtilis in complex with a 12 base palindromic DNA fragment at a resolution of 3.2 Å. This structure shows the stalk region and the essential N-terminal region (NTR) previously unseen in our DNA unbound structure. The flexible nature of the NTR in solution was confirmed using SAXS. Thermofluor studies performed to assess the stability of RecU in complex with the arms of an HJ indicate that it confers stability...
January 25, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/27799325/muts2-promotes-homologous-recombination-in-bacillus-subtilis
#15
Peter E Burby, Lyle A Simmons
Bacterial MutS proteins are subdivided into two families, MutS1 and MutS2. MutS1 family members recognize DNA replication errors during their participation in the well-characterized mismatch repair (MMR) pathway. In contrast to the well-described function of MutS1, the function of MutS2 in bacteria has remained less clear. In Helicobacter pylori and Thermus thermophilus, MutS2 has been shown to suppress homologous recombination. The role of MutS2 is unknown in the Gram-positive bacterium Bacillus subtilis In this work, we investigated the contribution of MutS2 to maintaining genome integrity in B...
January 15, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/27268429/casein-kinase1-like-protein2-regulates-actin-filament-stability-and-stomatal-closure-via-phosphorylation-of-actin-depolymerizing-factor
#16
Shuangshuang Zhao, Yuxiang Jiang, Yang Zhao, Shanjin Huang, Ming Yuan, Yanxiu Zhao, Yan Guo
The opening and closing of stomata are crucial for plant photosynthesis and transpiration. Actin filaments undergo dynamic reorganization during stomatal closure, but the underlying mechanism for this cytoskeletal reorganization remains largely unclear. In this study, we identified and characterized Arabidopsis thaliana casein kinase 1-like protein 2 (CKL2), which responds to abscisic acid (ABA) treatment and participates in ABA- and drought-induced stomatal closure. Although CKL2 does not bind to actin filaments directly and has no effect on actin assembly in vitro, it colocalizes with and stabilizes actin filaments in guard cells...
June 2016: Plant Cell
https://www.readbyqxmd.com/read/27131283/tuning-of-the-stability-and-energy-levels-of-singlet-exciton-fission-relevant-excited-states-of-pentacenes-by-site-specific-substitution
#17
Li Shen, Yuhan Chen, Xiyou Li, Chungang Li
Pentacene crystals or oligomers undergo efficient singlet exciton fission (SF) after photo-excitation, which is expected to be useful in overcoming the Shockley-Queisser theoretical limit of solar cells. However, pentacenes are extremely unstable in air due to oxidation by oxygen. In this work, we designed a group of pentacene compounds with different substituents at different positions. The energy levels of HOMO and LUMO, which are believed to be closely related to the stability of pentacene, were calculated...
May 2016: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/27085264/alterations-in-intraocular-cytokine-levels-following-intravitreal-ranibizumab
#18
Farzin Forooghian, Peter J Kertes, Kenneth T Eng, David A Albiani, Andrew W Kirker, Andrew B Merkur, Nader Fallah, Sijia Cao, Jing Cui, Chris Or, Joanne A Matsubara
OBJECTIVE: Our previous work has shown that, after intravitreal bevacizumab (IVB) administration, decreases in the levels of vascular endothelial growth factor (VEGF)-A and placental growth factor (PlGF), along with increases in the levels of interleukin (IL)-8 and transforming growth factor (TGF)-β2, can be observed. It is not yet known if similar changes occur after intravitreal ranibizumab (IVR). The purpose of this study was to examine intraocular cytokine changes after IVR. DESIGN: Prospective clinical study...
April 2016: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
https://www.readbyqxmd.com/read/27047566/comparison-between-intraperitoneal-and-intravenous-lidocaine-for-postoperative-analgesia-after-elective-abdominal-hysterectomy-a-double-blind-placebo-controlled-study
#19
Saghar Samimi, Arman Taheri, Fatemeh Davari Tanha
OBJECTIVE: To compare the efficacy of intravenous and intraperitoneal injection of lidocaine and normal saline in relieving postoperative pain after elective abdominal hysterectomy. MATERIALS AND METHODS: For this double-blind randomized controlled study 109 patients undergoing elective abdominal hysterectomy were randomly allocated to three groups :1) IV group (intravenous injection group) received intravenous lidocaine %2 bolus 1.5mg/kg 30 min before incision and then a continuous lidocaine infusion of 2mg/kg and before the wound closure an intraperitoneal injection of N/S , 2) IP group (intraperitoneal group) received intravenous N/S and intraperitoneal lidocaine 3mg/kg , 3) P group (placebo, N/S) received both intravenous and intraperitoneal N/S...
November 2015: Journal of Family & Reproductive Health
https://www.readbyqxmd.com/read/26877477/p-171-pregnancy-induced-hypertension-in-patients-with-mitral-stenosis
#20
N Benatta, L Hamou, D D Batouche, T Merzouk, M Benbouabdellah
OBJECTIFS: La prise en charge du rétrécissement mitral (RM) associé à l'hypertension gravidique continue à poser un défi au médecin particulièrement dans les pays en voie de développement. Le but de cette étude était d'étudier le profil clinique, thérapeutique et évolutif des RM accompagnés hypertension gravidique. MéTHODES: 83 patientes atteintes d'un rétrécissement mitral suivis durant la grossesse dont 7 patientes ont compliqué d'une Hypertension gravidique. RéSULTATS: Le diagnostic de l'Hypertension gravidique a été posé par la MAPA, le RM était serré dans les 7 cas avec une surface mitrale anatomique entre 1 et 1,5cm(2)...
December 2015: Annales de Cardiologie et D'angéiologie
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