keyword
MENU ▼
Read by QxMD icon Read
search

ubiquinol

keyword
https://www.readbyqxmd.com/read/28361965/transcriptomics-and-proteomic-studies-reveal-acaricidal-mechanism-of-octadecanoic-acid-3-4-tetrahydrofuran-diester-against-sarcoptes-scabiei-var-cuniculi
#1
Xu Song, Zhenzhen Chen, Renyong Jia, Mei Cao, Yuanfeng Zou, Lixia Li, Xiaoxia Liang, Lizi Yin, Changliang He, Guizhou Yue, Zhongqiong Yin
In our previous study, a new compound, octadecanoic acid-3, 4-tetrahydrofuran diester, possessing potent acaricidal activity was obtained from neem oil. This study performed RNA-seq transcriptomics and iTRAQ proteomics to uncover the acaricidal mechanism of the compound against Sarcoptes scabiei var. cuniculi. The results of transcriptomics indicated that after treatment with octadecanoic acid-3, 4-tetrahydrofuran diester, genes related to "Energy metabolism" were significantly up-/down-regulated, including citrate cycle, oxidative phosphorylation pathway and fatty acid metabolism...
March 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28332314/the-mitochondrial-hinge-protein-uqcrh-is-a-novel-prognostic-factor-for-hepatocellular-carcinoma
#2
Eun-Ran Park, Sang-Bum Kim, Jee-San Lee, Yang-Hyun Kim, Dong-Hyoung Lee, Eung-Ho Cho, Sun-Hoo Park, Chul Ju Han, Bu-Yeo Kim, Dong Wook Choi, Young Do Yoo, Ami Yu, Jae Won Lee, Ja June Jang, Young Nyun Park, Kyung-Suk Suh, Kee-Ho Lee
Alterations in mitochondrial respiration contribute to the development and progression of cancer via abnormal biogenesis, including generation of reactive oxygen species. Ubiquinol-cytochrome c reductase hinge protein (UQCRH) consists of the cytochrome bc1 complex serving respiration in mitochondria. In the present study, we analyzed UQCRH abnormalities in hepatocellular carcinoma (HCC) and its association with clinical outcomes of patients. UQCRH expression in HCC was determined via semiquantitative and quantitative real-time reverse transcriptase polymerase chain reaction of 96 surgically resected HCC tissues positive for hepatitis B virus surface antigen...
April 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28241731/the-q-cycle-mechanism-of-the-bc1-complex-a-biologist-s-perspective-on-atomistic-studies
#3
Antony R Crofts, Stuart W Rose, Rodney L Burton, Amit V Desai, Paul J A Kenis, Sergei A Dikanov
The Q-cycle mechanism of the bc1 complex is now well enough understood to allow application of advanced computational approaches to the study of atomistic processes. In addition to the main features of the mechanism, these include control and gating of the bifurcated reaction at the Qo-site, through which generation of damaging reactive oxygen species is minimized. We report a new molecular dynamics model of the Rhodobacter sphaeroides bc1 complex implemented in a native membrane, and constructed so as to eliminate blemishes apparent in earlier Rhodobacter models...
April 20, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28235459/searching-for-the-low-affinity-ubiquinone-binding-site-in-cytochrome-bo3-from-escherichia-coli
#4
Sylvia K Choi, Myat T Lin, Hanlin Ouyang, Robert B Gennis
The cytochrome bo3 ubiquinol oxidase is one of three respiratory oxygen reductases in the aerobic respiratory chain of Escherichia coli. The generally accepted model of catalysis assumes that cyt bo3 contains two distinct ubiquinol binding sites: (i) a low affinity (QL) site which is the traditional substrate binding site; and (ii) a high affinity (QH) site where a "permanently" bound quinone acts as a cofactor, taking two electrons from the substrate quinol and passing them one-by-one to the heme b component of the enzyme which, in turn, transfers them to the heme o3/CuB active site...
February 21, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28150130/three-year-follow-up-of-high-dose-ubiquinol-supplementation-in-a-case-of-familial-multiple-system-atrophy-with-compound-heterozygous-coq2-mutations
#5
Jun Mitsui, Ken Koguchi, Toshimitsu Momose, Miwako Takahashi, Takashi Matsukawa, Tsutomu Yasuda, Shin-Ichi Tokushige, Hiroyuki Ishiura, Jun Goto, Shigeaki Nakazaki, Tomoyoshi Kondo, Hidefumi Ito, Yorihiro Yamamoto, Shoji Tsuji
We report a 3-year follow-up of high-dose ubiquinol supplementation in a case of familial multiple system atrophy (MSA) with compound heterozygous nonsense (R387X) and missense (V393A) mutations in COQ2. A high-dose ubiquinol supplementation substantially increased total coenzyme Q10 levels in cerebrospinal fluid as well as in plasma. The patient was at the advanced stage of MSA, and the various scores of clinical rating scales remained stable without changes during the 3 years. The cerebral metabolic ratio of oxygen measured by (15)O2 PET, however, increased by approximately 30% after administration of ubiquinol, suggesting that ubiquinol can improve mitochondrial oxidative metabolism in the brain...
February 1, 2017: Cerebellum
https://www.readbyqxmd.com/read/28127420/permanent-draft-genome-of-thiobacillus-thioparus-dsm-505-t-an-obligately-chemolithoautotrophic-member-of-the-betaproteobacteria
#6
Lee P Hutt, Marcel Huntemann, Alicia Clum, Manoj Pillay, Krishnaveni Palaniappan, Neha Varghese, Natalia Mikhailova, Dimitrios Stamatis, Tatiparthi Reddy, Chris Daum, Nicole Shapiro, Natalia Ivanova, Nikos Kyrpides, Tanja Woyke, Rich Boden
Thiobacillus thioparus DSM 505(T) is one of first two isolated strains of inorganic sulfur-oxidising Bacteria. The original strain of T. thioparus was lost almost 100 years ago and the working type strain is Culture C(T) (=DSM 505(T) = ATCC 8158(T)) isolated by Starkey in 1934 from agricultural soil at Rutgers University, New Jersey, USA. It is an obligate chemolithoautotroph that conserves energy from the oxidation of reduced inorganic sulfur compounds using the Kelly-Trudinger pathway and uses it to fix carbon dioxide It is not capable of heterotrophic or mixotrophic growth...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/28127418/tualang-honey-protects-the-rat-midbrain-and-lung-against-repeated-paraquat-exposure
#7
Suk Peng Tang, Sirajudeen Kuttulebbai Nainamohamed Salam, Hasnan Jaafar, Siew Hua Gan, Mustapha Muzaimi, Siti Amrah Sulaiman
Paraquat (PQ) is a dopaminergic neurotoxin and a well-known pneumotoxicant that exerts its toxic effect via oxidative stress-mediated cellular injuries. This study investigated the protective effects of Tualang honey against PQ-induced toxicity in the midbrain and lungs of rats. The rats were orally treated with distilled water (2 mL/kg/day), Tualang honey (1.0 g/kg/day), or ubiquinol (0.2 g/kg/day) throughout the experimental period. Two weeks after the respective treatments, the rats were injected intraperitoneally with saline (1 mL/kg/week) or PQ (10 mg/kg/week) once per week for four consecutive weeks...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28099963/low-molecular-weight-hydrophilic-and-lipophilic-antioxidants-in-nonmelanoma-skin-carcinomas-and-adjacent-normal-looking-skin
#8
COMPARATIVE STUDY
Konstantina Grammenandi, Maria Kyriazi, Alexandra Katsarou-Katsari, Othon Papadopoulos, Ioanna Anastassopoulou, Georgios T Papaioannou, Alexandros Sagriotis, Michail Rallis, Howard I Maibach
Low-molecular-weight antioxidants are some of the most efficient agents of the skin defense mechanism against environmental factors, such as cosmic rays, smoke, and pollutants. The total skin concentrations of hydrophilic ascorbic and uric acids, as well as lipophilic α-tocopherol, β-carotene, and ubiquinol-10 antioxidants were determined by an HPLC-EC detector from 18 biopsies of human nonmelanoma skin carcinomas and 18 biopsies from skin areas adjacent to carcinomas. No significant differences in the concentrations of lipophilic antioxidants in both carcinomas and normal-looking skin areas adjacent to carcinomas were observed...
2016: Skin Pharmacology and Physiology
https://www.readbyqxmd.com/read/28088729/new-insights-into-aox2-transcriptional-regulation-in-chlamydomonas-reinhardtii
#9
Mariya Ostroukhova, Zhanneta Zalutskaya, Elena Ermilova
A feature of the mitochondrial electron transport chain in plants, some protists and many fungi is the presence of two terminal oxidases, the energy-conserving cytochrome oxidase and another termed alternative oxidase (AOX). AOX branches from the main respiratory chain, directly coupling the oxidation of ubiquinol with reduction of oxygen to water. The AOX genes can be divided into two discrete subfamilies, AOX1 and AOX2. Although AOX has been proposed to play essential roles in stress tolerance of organisms, the role of subfamily AOX2 is largely unknown...
April 2017: European Journal of Protistology
https://www.readbyqxmd.com/read/28067626/broad-aox-expression-in-a-genetically-tractable-mouse-model-does-not-disturb-normal-physiology
#10
Marten Szibor, Praveen K Dhandapani, Eric Dufour, Kira M Holmström, Yuan Zhuang, Isabelle Salwig, Ilka Wittig, Juliana Heidler, Zemfira Gizatullina, Timur Gainutdinov, Helmut Fuchs, Valérie Gailus-Durner, Martin Hrabě de Angelis, Jatin Nandania, Vidya Velagapudi, Astrid Wietelmann, Pierre Rustin, Frank N Gellerich, Howard T Jacobs, Thomas Braun
Plants and many lower organisms, but not mammals, express alternative oxidases (AOXs) that branch the mitochondrial respiratory chain, transferring electrons directly from ubiquinol to oxygen without proton pumping. Thus, they maintain electron flow under conditions when the classical respiratory chain is impaired, limiting excess production of oxygen radicals and supporting redox and metabolic homeostasis. AOX from Ciona intestinalis has been used to study and mitigate mitochondrial impairments in mammalian cell lines, Drosophila disease models and, most recently, in the mouse, where multiple lentivector-AOX transgenes conferred substantial expression in specific tissues...
February 1, 2017: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/28060946/high-osmolarity-environments-activate-the-mitochondrial-alternative-oxidase-in-debaryomyces-hansenii
#11
Wilson Garcia-Neto, Alfredo Cabrera-Orefice, Salvador Uribe-Carvajal, Alicia J Kowaltowski, Luis Alberto Luévano-Martínez
The oleaginous yeast Debaryomyces hansenii is a good model to understand molecular mechanisms involved in halotolerance because of its impressive ability to survive under a wide range of salt concentrations. Several cellular adaptations are implicated in this response, including the presence of a cyanide-insensitive ubiquinol oxidase (Aox). This protein, which is present in several taxonomical orders, has been related to different stress responses. However, little is known about its role in mitochondria during transitions from low to high saline environments...
2017: PloS One
https://www.readbyqxmd.com/read/27987212/the-occurrence-and-control-of-nitric-oxide-generation-by-the-plant-mitochondrial-electron-transport-chain
#12
Nicole A Alber, Hampavi Sivanesan, Greg C Vanlerberghe
The plant mitochondrial electron transport chain (ETC) is bifurcated such that electrons from ubiquinol are passed to oxygen via the usual cytochrome path or through alternative oxidase (AOX). We previously showed that knockdown of AOX in transgenic tobacco increased leaf concentrations of nitric oxide (NO), implying that an activity capable of generating NO had been effected. Here we identify the potential source of this NO. Treatment of leaves with antimycin A (AA, Qi -site inhibitor of Complex III) increased NO amount more than treatment with myxothiazol (Myxo, Qo -site inhibitor) despite both being equally effective at inhibiting respiration...
December 16, 2016: Plant, Cell & Environment
https://www.readbyqxmd.com/read/27986792/alternative-oxidase-transcription-factors-aod2-and-aod5-of-neurospora-crassa-control-the-expression-of-genes-involved-in-energy-production-and-metabolism
#13
Zhigang Qi, Kristina M Smith, Erin L Bredeweg, Natasa Bosnjak, Michael Freitag, Frank E Nargang
In Neurospora crassa, blocking the function of the standard mitochondrial electron transport chain results in the induction of an alternative oxidase (AOX). AOX transfers electrons directly from ubiquinol to molecular oxygen. AOX serves as a model of retrograde regulation since it is encoded by a nuclear gene that is regulated in response to signals from mitochondria. The N. crassa transcription factors AOD2 and AOD5 are necessary for the expression of the alternative oxidase gene. To gain insight into the mechanism by which these factors function, and to determine if they have roles in the expression of additional genes in N...
December 16, 2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/27909649/in-silico-profiling-of-cell-growth-and-succinate-production-in-escherichia-coli-nzn111
#14
Xingxing Jian, Ningchuan Li, Cheng Zhang, Qiang Hua
BACKGROUND: Succinic acid is a valuable product due to its wide-ranging utilities. To improve succinate production and reduce by-products formation, Escherichia coli NZN111 was constructed by insertional inactivation of lactate dehydrogenase (LDH) and pyruvate formate lyase (PFL) encoded by the genes ldhA and pflB, respectively. However, this double-deletion mutant is incapable of anaerobically growing on glucose in rich or minimal medium even with acetate supplementation. A widespread hold view is that the inactivation of NADH-dependent LDH limits the regeneration of NAD(+) and consequently disables proper growth under anaerobic conditions...
2016: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/27907044/coenzyme-q10-status-as-a-determinant-of-muscular-strength-in-two-independent-cohorts
#15
Alexandra Fischer, Simone Onur, Petra Niklowitz, Thomas Menke, Matthias Laudes, Gerald Rimbach, Frank Döring
Aging is associated with sarcopenia, which is a loss of skeletal muscle mass and function. Coenzyme Q10 (CoQ10) is involved in several important functions that are related to bioenergetics and protection against oxidative damage; however, the role of CoQ10 as a determinant of muscular strength is not well documented. The aim of the present study was to evaluate the determinants of muscular strength by examining hand grip force in relation to CoQ10 status, gender, age and body mass index (BMI) in two independent cohorts (n = 334, n = 967)...
2016: PloS One
https://www.readbyqxmd.com/read/27845902/systematic-expression-analysis-of-the-mitochondrial-complex-iii-subunits-identifies-uqcrc1-as-biomarker-in-clear-cell-renal-cell-carcinoma
#16
Jörg Ellinger, Arabella Gromes, Mirjam Poss, Maria Brüggemann, Doris Schmidt, Nadja Ellinger, Yuri Tolkach, Dimo Dietrich, Glen Kristiansen, Stefan C Müller
Mitochondrial dysfunction is common in cancer, and the mitochondrial electron transport chain is often affected in carcinogenesis. So far, few is known about the expression of the mitochondrial complex III (ubiquinol-cytochrome c reductase complex) subunits in clear cell renal cell carcinoma (ccRCC). In this study, the NextBio database was used to determine an expression profile of the mitochondrial complex III subunits based on published microarray studies. We observed that five out of 11 subunits of the complex III were downregulated in at least three microarray studies...
December 27, 2016: Oncotarget
https://www.readbyqxmd.com/read/27836196/optimization-of-benzoquinone-and-hydroquinone-derivatives-as-potent-inhibitors-of-human-5-lipoxygenase
#17
Antonella Peduto, Maria Scuotto, Verena Krauth, Fiorentina Roviezzo, Antonietta Rossi, Veronika Temml, Veronica Esposito, Hermann Stuppner, Daniela Schuster, Bruno D'Agostino, Chiara Schiraldi, Mario de Rosa, Oliver Werz, Rosanna Filosa
Aiming to assess the biological activities of synthetic 1,4-benzoquinones, we previously synthesized different libraries of benzoquinones with lipophilic and bulky alkyl- or aryl-substituents that inhibited 5-lipoxygenase (5-LO). The high potency of 4,5-dimethoxy-3-alkyl-1,2-benzoquinones on 5-LO led to the idea to further modify the structures and thus to improve the inhibitory potential in vitro and in vivo as well as to investigate SARs. Systematic structural optimization through accurate structure-based design resulted in compound 30 (3-tridecyl-4,5-dimethoxybenzene-1,2-diol), an ubiquinol derivative that exhibited the strongest anti-inflammatory effect, with a 10-fold improved 5-LO inhibitory activity (IC50 = 28 nM) in activated neutrophils...
February 15, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27834404/small-regulatory-rnas-of-rickettsia-conorii
#18
Hema P Narra, Casey L C Schroeder, Abha Sahni, Mark Rojas, Kamil Khanipov, Yuriy Fofanov, Sanjeev K Sahni
Small regulatory RNAs comprise critically important modulators of gene expression in bacteria, yet very little is known about their prevalence and functions in Rickettsia species. R. conorii, the causative agent of Mediterranean spotted fever, is a tick-borne pathogen that primarily infects microvascular endothelium in humans. We have determined the transcriptional landscape of R. conorii during infection of Human Microvascular Endothelial Cells (HMECs) by strand-specific RNA sequencing to identify 4 riboswitches, 13 trans-acting (intergenic), and 22 cis-acting (antisense) small RNAs (termed 'Rc_sR's)...
November 11, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27816999/heterologous-expression-of-the-crassostrea-gigas-pacific-oyster-alternative-oxidase-in-the-yeast-saccharomyces-cerevisiae
#19
Aaron Robertson, Kyle Schaltz, Karina Neimanis, James F Staples, Allison E McDonald
Alternative oxidase (AOX) is a terminal oxidase within the inner mitochondrial membrane (IMM) present in many organisms where it functions in the electron transport system (ETS). AOX directly accepts electrons from ubiquinol and is therefore capable of bypassing ETS Complexes III and IV. The human genome does not contain a gene coding for AOX, so AOX expression has been suggested as a gene therapy for a range of human mitochondrial diseases caused by genetic mutations that render Complex III and/or IV dysfunctional...
October 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27810396/respiratory-complex-ii-ros-production-and-the-kinetics-of-ubiquinone-reduction
#20
Vera G Grivennikova, Vladimir S Kozlovsky, Andrei D Vinogradov
Bovine heart mitochondrial respiratory complex II generates ROS, mostly as superoxide, at the rate of about 20% of that detected during simultaneous operation of complex I and II when oxidation of ubiquinol is prevented by myxothiazol. ROS generating activity at different fumarate/succinate concentrations ratio implies that an enzyme component with a midpoint potential 40mV more positive than that of fumarate/succinate couple is the donor for one-electron reduction of oxygen. This suggests that the iron-sulfur cluster(s) is(are) involved in superoxide formation...
February 2017: Biochimica et Biophysica Acta
keyword
keyword
36510
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"