Safoura Izanlou, Alireza Afshar, Afshin Zare, Kulyash R Zhilisbayeva, Shabnam Bakhshalizadeh, Zahra Safaei, Soheila Sehat-Bakhsh, Sajed Khaledi, Hamid-Reza Asgari, Somaieh Kazemnejad, Mansoureh Ajami, Monireh Ajami, Masoumeh Dehghan Tarzjani, Vahid Najafzadeh, Mohammad Reza Kouchakian, Nadiar M Mussin, Asset A Kaliyev, Raisa A Aringazina, Mahdi Mahdipour, Reza Shirazi, Amin Tamadon
Three-dimensional nanofiber scaffolds offer a promising method for simulating in vivo conditions within the laboratory. This study aims to investigate the influence of a bilayer amniochorionic membrane/nanofibrous fibroin scaffold on the differentiation of human menstrual blood mesenchymal stromal/stem cells (MenSCs) into female germ cells. MenSCs were isolated and assigned to four culture groups: (i) MenSCs co-cultured with granulosa cells (GCs) using the scaffold (3D-T group), (ii) MenSCs using the scaffold alone (3D-C group), (iii) MenSCs co-cultured only with GCs (2D-T group), and (iv) MenSCs without co-culture or scaffold (2D-C group)...
September 11, 2023: Tissue & Cell