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https://www.readbyqxmd.com/read/28526652/retinoic-acid-induces-differentiation-of-buffalo-bubalus-bubalis-embryonic-stem-cells-into-germ-cells
#1
Syed Mohmad Shah, Suresh Kumar Singla, Prabhat Palta, Radhey Sham Manik, Manmohan Singh Chauhan
Development of precise and reproducible culture system for in vitro differentiation of embryonic stem (ES) cells into germ cells counts as a major leap forward for understanding not only the remarkable process of gametogenesis, otherwise obscured by limited availability of precursor primordial germ cells (PGCs), but in finally treating the catastrophic infertility. Taking into account the significant role of retinoic acid (RA) during in vivo gametogenesis, we designed the present study to investigate the effects of its stimulation on directing the differentiation of ES cells into germ cells...
May 16, 2017: Gene
https://www.readbyqxmd.com/read/28506985/histone-acetyltransferase-kat8-is-essential-for-mouse-oocyte-development-by-regulating-ros-levels
#2
Shi Yin, Xiaohua Jiang, Hanwei Jiang, Qian Gao, Fang Wang, Suixing Fan, Teka Khan, Nazish Jabeen, Manan Khan, Asim Ali, Peng Xu, Tej K Pandita, Heng-Yu Fan, Yuanwei Zhang, Qinghua Shi
Proper oocyte development is critical for female fertility and requires timely and accurate control of gene expression. K (Lysine) Acetyltransferase 8 (KAT8), an important component of the X chromosome dosage compensation system in Drosophila, regulates gene activity by acetylating histone H4 preferentially at lysine 16. To explore the function of Kat8 during mouse oocyte development, we crossed Kat8(flox/flox) mice with Gdf9-Cre mice to specifically delete Kat8 in oocytes. Oocyte Kat8 deletion resulted in female infertility with follicle development failure in the secondary and preantral follicle stages...
May 15, 2017: Development
https://www.readbyqxmd.com/read/28506298/fecx-bar-a-novel-bmp15-mutation-responsible-for-prolificacy-and-female-sterility-in-tunisian-barbarine-sheep
#3
Narjess Lassoued, Zohra Benkhlil, Florent Woloszyn, Ahmed Rejeb, Mohamed Aouina, Mourad Rekik, Stephane Fabre, Sonia Bedhiaf-Romdhani
BACKGROUND: Naturally occurring mutations in growth and differentiation factor 9 (GDF9) or bone morphogenetic protein 15 (BMP15) genes are associated with increased ovulation rate (OR) and litter size (LS) but also sterility. Observing the Tunisian Barbarine ewes of the "W" flock selected for improved prolificacy, we found prolific and infertile ewes with streaky ovaries. Blood genomic DNA was extracted from a subset of low-ovulating, prolific and infertile ewes of the "W" flock, and the entire coding sequences of GDF9 and BMP15 were sequenced...
May 15, 2017: BMC Genetics
https://www.readbyqxmd.com/read/28481036/generation-and-characterization-of-a-conditional-allele-of-interferon-regulatory-factor-6
#4
Arianna L Smith, Youssef A Kousa, Akira Kinoshita, Kate Fodor, Baoli Yang, Brian C Schutte
Interferon Regulatory Factor 6 (IRF6) is a critical regulator of differentiation, proliferation and migration of keratinocytes. Mutations in IRF6 cause two autosomal dominant disorders characterized by cleft lip with or without cleft palate. In addition, DNA variation in IRF6 confers significant risk for non-syndromic cleft lip and palate. IRF6 is also implicated in adult onset development and disease processes, including mammary gland development and squamous cell carcinoma. Mice homozygous for a null allele of Irf6 die shortly after birth due to severe skin, limb, and craniofacial defects, thus impeding the study of gene function after birth...
May 8, 2017: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/28451989/tissue-engineered-human-amniotic-membrane-application-in-mouse-ovarian-follicular-culture
#5
M Motamed, Z Sadr, M R Valojerdi, A Moini, S Oryan, M Totonchi, B Ebrahimi, S Maroufizadeh, E Taghiabadi, R Fathi
Since folliculogenesis requires a powerful cell-matrix interaction, natural scaffolds seem to be needed for follicular culture. Human amniotic membrane (HAM) offers promise as a support of in vitro ovarian follicular culture. HAM was decellularized with trypsin and EDTA. DNA and histology assays were performed to determine the elimination rate of genomic components. Cyto-biocompatibility of decellular AM (DAM) was verified by the cell viability (MTT) test. The small parts of intact amniotic membrane (IAM) and DAM were coated on the bottom of 96-well and each well was filled with 150 µL of base medium...
April 27, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28420820/high-insulin-impaired-ovarian-function-in-early-pregnant-mice-and-the-role-of-autophagy-in-this-process
#6
Yan Su, Juan Wu, Junlin He, Xueqing Liu, Xuemei Chen, Yubin Ding, Chen Zhang, Wenqi Chen, Yingxiong Wang, Rufei Gao
Metabolic disorders, such as PCOS (polycystic ovarian syndrome) and T2DM (type 2 diabetes mellitus), are associated with menstrual dysfunction, anovulation, infertility, and early pregnancy loss. Ovarian dysfunction is not only related to low pregnancy rates but also to the increased risk of miscarriage. Women with PCOS or T2DM, characterized by hyperinsulinemia, commonly experience ovarian dysfunction. In this study, we first explored whether high insulin levels directly affected ovarian functioning during embryo implantation...
April 18, 2017: Endocrine Journal
https://www.readbyqxmd.com/read/28420163/beneficial-effects-of-melatonin-on-the-in-vitro-maturation-of-sheep-oocytes-and-its-relation-to-melatonin-receptors
#7
Xiuzhi Tian, Feng Wang, Lu Zhang, Changjiu He, Pengyun Ji, Jing Wang, Zhenzhen Zhang, Dongying Lv, Wusiman Abulizi, Xuguang Wang, Zhengxing Lian, Guoshi Liu
(1) Background: The binding sites of melatonin, as a multifunctional molecule, have been identified in human, porcine, and bovine samples. However, the binding sites and mechanisms of melatonin have not been reported in sheep; (2) Methods: Cumulus-oocyte complexes (COCs) were cultured in TCM-199 supplemented with melatonin at concentrations of 0, 10(-3), 10(-5), 10(-7), 10(-9), and 10(-11) M. Melatonin receptors (MT1 and MT2) were evaluated via immunofluorescence and Western blot. The effects of melatonin on cumulus cell expansion, nuclear maturation, embryo development, and related gene (GDF9, DNMT1, PTX3, HAS2, and EGFR) expression were investigated...
April 17, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28377898/bmp15-and-gdf9-gene-mutations-in-premature-ovarian-failure
#8
Ravindra Kumar, Madhuri Alwani, Susmit Kosta, Ravjyot Kaur, Sarita Agarwal
BACKGROUND: Premature ovarian failure (POF) is an ovarian defect characterized by the premature depletion of ovarian follicles before the age of 40, representing one major cause of female infertility. Mutations in bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) have been shown to be associated with POF. METHODS: Genomic DNA was isolated from 52 idiopathic premature ovarian failure patients and 100 normal control individuals. Exons of BMP15 and GDF9 gene were amplified using PCR method and subjected to directed sequencing...
January 2017: Journal of Reproduction & Infertility
https://www.readbyqxmd.com/read/28377895/short-term-culture-of-vitrified-human-ovarian-cortical-tissue-to-assess-the-cryopreservation-outcome-molecular-and-morphological-analysis
#9
Mehdi Ramezani, Mojdeh Salehnia, Mina Jafarabadi
BACKGROUND: The aim of the present study was to evaluate the effectiveness of human ovarian vitrification protocol followed with in vitro culture at the morphological and molecular levels. METHODS: Ovarian tissues were obtained from 10 normal transsexual women and cut into small pieces and were divided into non-vitrified and vitrified groups and some of the tissues fragments in both groups were randomly cultured for two weeks. The morphological study using hematoxylin and eosin and Masson's trichrome staining was done...
January 2017: Journal of Reproduction & Infertility
https://www.readbyqxmd.com/read/28359091/bmp15-mutations-associated-with-primary-ovarian-insufficiency-reduce-expression-activity-or-synergy-with-gdf9
#10
Liliana C Patiño, Kelly L Walton, Thomas D Mueller, Katharine E Johnson, William Stocker, Dulama Richani, David Agapiou, Robert B Gilchrist, Paul Laissue, Craig A Harrison
Context: Bone morphogenetic protein (BMP)15 is an oocyte-specific growth factor, which, together with growth differentiation factor (GDF) 9, regulates folliculogenesis and ovulation rate. Multiple mutations in BMP15 have been identified in women with primary ovarian insufficiency (POI), supporting a pathogenic role; however, the underlying biological mechanism of many of these mutants remains unresolved. Objectives: To determine how mutations associated with ovarian dysfunction alter the biological activity of human BMP15...
March 1, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28332739/gdf9-affects-the-development-and-tight-junction-functions-of-immature-bovine-sertoli-cells
#11
K Tang, L Wang, Y Jin, W Yang, L Yang
Transforming growth factor-β (TGFβ) superfamily are critical regulators of germ cell development that act as extracellular ligands of the signal transduction pathways regulating proliferation, apoptosis and other aspects of cell behaviour. As a member of the TGF-β superfamily, growth differentiation factor 9 (GDF9) plays a critical role in ovarian follicular development and the ovulation rate in females; however, its role in the testis has not been well elucidated. The aim of this study was to investigate the expression of GDF9 and its receptor genes BMPRII and ALK5 in prepuberal bovine Sertoli cells (SCs)...
March 23, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28300526/effects-of-bone-morphogenetic-protein-4-bmp4-on-in-vitro-development-and-survival-of-bovine-preantral-follicles-enclosed-in-fragments-ovarian-tissue
#12
Ellen de Vasconcelos da Cunha, Glaucinete Borges de Souza, José Renato de Sousa Passos, Anderson Weiny Barbalho Silva, Andressa Minussi Dau, Márcia Viviane Alves Saraiva, Raimundo Nonato Braga Lobo, José Roberto Viana Silva
The aim of this study was to evaluate the effects of different concentrations of BMP4 on activation, development and mRNA expression of GDF9, BMP15, PCNA, Bax and Bcl2 in cultured bovine follicles enclosed in ovarian tissues. Ovarian tissue fragments were cultured for 6 days in α-MEM+ alone or supplemented with different concentrations of BMP4 (10, 50 or 100 ng/ml). Classical histology was performed to analyze follicle growth and morphology, while real-time PCR was used to analyze mRNA levels in fresh and cultured tissues...
March 16, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28244827/deriving-cells-expressing-markers-of-female-germ-cells-from-premature-ovarian-failure-patient-specific-induced-pluripotent-stem-cells
#13
Yanfei Wen, Wen He, Manbo Jiang, Minhui Zeng, Liuhong Cai
AIM: We proposed a two-step protocol for deriving cells expressing markers of female germ cells (FGCs) from premature ovarian failure patient-specific induced pluripotent stem cells (POF-iPSCs). MATERIAL & METHODS: We cultured POF-iPSCs in suspension and pretreated them with TGFβ-1 (1 ng/ml) for 2 days and continued with both TGFβ-1 and BMP4 (50 ng/ml) for 5 more days. Then changed to media containing retinoic acid (1 μM) and 5% follicular fluid for another 7 days...
March 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28244110/the-p2x7-receptor-links-mechanical-strain-to-cytokine-il-6-up-regulation-and-release-in-neurons-and-astrocytes
#14
Wennan Lu, Farraj Albalawi, Jonathan M Beckel, Jason C Lim, Alan M Laties, Claire H Mitchell
Mechanical strain in neural tissues can lead to the up-regulation and release of multiple cytokines including interleukin 6 (IL-6). In the retina, the mechanosensitive release of ATP can autostimulate P2X7 receptors on both retinal ganglion cell neurons and optic nerve head astrocytes. Here, we asked whether the purinergic signaling contributed to the IL-6 response to increased intraocular pressure (IOP) in vivo, and stretch or swelling in vitro. Rat and mouse eyes were exposed to non-ischemic elevations in IOP to 50-60 mmHg for 4 h...
May 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28215684/expression-of-genes-involved-in-bmp-and-estrogen-signaling-and-ampk-production-can-be-important-factors-affecting-total-number-of-antral-follicles-in-ewes
#15
Golshan Foroughinia, Ali Fazileh, Shahin Eghbalsaied
Follicular growth and ovulation of healthy oocytes is a complicated process which is regulated by several endocrine and paracrine factors as well as cross-talk between the oocyte and its surrounding somatic cells. This study compared the expression profile of some candidate genes involved in BMP signaling as well as estrogen and AMPK production in cumulus-oocyte complex (COC) of small and large antral follicles and their associated somatic cell layers in ovaries from ewes with high- and low-antral follicle count (AFC)...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28214889/regulatory-role-of-mirna-375-in-expression-of-bmp15-gdf9-receptors-and-its-effect-on-proliferation-and-apoptosis-of-bovine-cumulus-cells
#16
Hongyan Chen, Chang Liu, Hao Jiang, Yan Gao, Mingqiang Xu, Jiawei Wang, Siyuan Liu, Yao Fu, Xulei Sun, Jiajun Xu, Jiabao Zhang, Lisheng Dai
BACKGROUND: Bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) are members of the transforming growth factor beta (TGF-β) superfamily. Through autocrine and paracrine mechanisms, these two factors can regulate cell differentiation, proliferation, and other functions in the ovary locally. Furthermore, GDF9 and BMP15 play vital roles in follicular growth, atresia, ovulation, fertilization, reproduction, and maintenance. Numerous studies have demonstrated a synergy between BMP15 and GDF9...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28203731/expression-analysis-of-growth-differentiation-factor-9-gdf9-gdf9-anti-m%C3%A3-llerian-hormone-amh-amh-and-aromatase-cyp19a1a-cyp19a1a-during-gonadal-differentiation-of-the-zebrafish-danio-rerio%C3%A2
#17
https://www.readbyqxmd.com/read/28112985/comparison-of-human-amniotic-chorionic-and-umbilical-cord-multipotent-mesenchymal-stem-cells-regarding-their-capacity-for-differentiation-toward-female-germ-cells
#18
Hamid Reza Asgari, Mohammad Akbari, Hossein Yazdekhasti, Zahra Rajabi, Shadan Navid, Fereshte Aliakbari, Niloufar Abbasi, Freidon Sargolzaei Aval, Alireza Shams, Mehdi Abbasi
Placenta harbors a plentiful source of various cells with stem cells or stem-like cell properties, which can be used in therapeutic procedures and research. Mesenchymal stem cells (MSCs) have attracted much attention due to their specific differentiation potential and tolerogenic properties. MSCs have been isolated from different parts of placenta; however, in this study, we isolated MSCs from amnion and chorion membrane, as well as umbilical cord (Wharton's jelly [WJ]) and compared their capacity regarding differentiation toward female germ cells under influence of 10 ng/mL BMP4...
February 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28093199/effect-of-different-cryo-devices-on-in%C3%A2-vitro-maturation-and-development-of-vitrified-warmed-immature-buffalo-oocytes
#19
Yelisetti Uma Mahesh, Henderson Rose Winnie Gibence, Sisinthy Shivaji, Brahmasani Sambasiva Rao
The aim of the study was to identify a cryo-device that would be best suited for the vitrification of buffalo immature cumulus-oocyte complexes (COCs) as judged by viability and meiotic competence of the vitrified-warmed oocytes and their development ability following in vitro fertilization (IVF). The expression of oocyte secreting factors and their receptors (GDF9, BMP15, BMPR2, TGFBR1) and apoptosis related genes (BCL2, BAX, P53, C-MYC) were compared in vitrified-warmed oocytes after in vitro maturation...
April 2017: Cryobiology
https://www.readbyqxmd.com/read/28087314/a-protective-role-of-cumulus-cells-after-short-term-exposure-of-rat-cumulus-cell-oocyte-complexes-to-lifestyle-or-environmental-contaminants
#20
Kelly A Campen, Kenneth P McNatty, Janet L Pitman
Ovarian follicular fluid provides a potential reservoir for exogenous compounds that may adversely affect oocyte quality. This study examined the effects of common lifestyle and environmental contaminants, namely bisphenol-A (BPA), caffeine, 3,4-methylenedioxymethamphetamine (MDMA), nicotine and Δ(9)-tetrahydrocannabinol (THC) on gap junction genes (Gja1, Gja4) and proteins (GJA1), glucose metabolism genes (Gfpt1, Pfkp) and oocyte growth factor genes (Bmp15, Gdf9), as well as gap junction transfer rate, in rat cumulus-oocyte complexes (COCs)...
January 10, 2017: Reproductive Toxicology
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