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https://www.readbyqxmd.com/read/28215684/expression-of-genes-involved-in-bmp-and-estrogen-signaling-and-ampk-production-can-be-important-factors-affecting-total-number-of-antral-follicles-in-ewes
#1
Golshan Foroughinia, Ali Fazileh, Shahin Eghbalsaied
Follicular growth and ovulation of healthy oocytes is a complicated process which is regulated by several endocrine and paracrine factors as well as cross-talk between the oocyte and its surrounding somatic cells. This study compared the expression profile of some candidate genes involved in BMP signaling as well as estrogen and AMPK production in cumulus-oocyte complex (COC) of small and large antral follicles and their associated somatic cell layers in ovaries from ewes with high- and low-antral follicle count (AFC)...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28214889/regulatory-role-of-mirna-375-in-expression-of-bmp15-gdf9-receptors-and-its-effect-on-proliferation-and-apoptosis-of-bovine-cumulus-cells
#2
Hongyan Chen, Chang Liu, Hao Jiang, Yan Gao, Mingqiang Xu, Jiawei Wang, Siyuan Liu, Yao Fu, Xulei Sun, Jiajun Xu, Jiabao Zhang, Lisheng Dai
BACKGROUND: Bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) are members of the transforming growth factor beta (TGF-β) superfamily. Through autocrine and paracrine mechanisms, these two factors can regulate cell differentiation, proliferation, and other functions in the ovary locally. Furthermore, GDF9 and BMP15 play vital roles in follicular growth, atresia, ovulation, fertilization, reproduction, and maintenance. Numerous studies have demonstrated a synergy between BMP15 and GDF9...
January 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28203731/expression-analysis-of-growth-differentiation-factor-9-gdf9-gdf9-anti-m%C3%A3-llerian-hormone-amh-amh-and-aromatase-cyp19a1a-cyp19a1a-during-gonadal-differentiation-of-the-zebrafish-danio-rerio%C3%A2
#3
https://www.readbyqxmd.com/read/28112985/comparison-of-human-amniotic-chorionic-and-umbilical-cord-multipotent-mesenchymal-stem-cells-regarding-their-capacity-for-differentiation-toward-female-germ-cells
#4
Hamid Reza Asgari, Mohammad Akbari, Hossein Yazdekhasti, Zahra Rajabi, Shadan Navid, Fereshte Aliakbari, Niloufar Abbasi, Freidon Sargolzaei Aval, Alireza Shams, Mehdi Abbasi
Placenta harbors a plentiful source of various cells with stem cells or stem-like cell properties, which can be used in therapeutic procedures and research. Mesenchymal stem cells (MSCs) have attracted much attention due to their specific differentiation potential and tolerogenic properties. MSCs have been isolated from different parts of placenta; however, in this study, we isolated MSCs from amnion and chorion membrane, as well as umbilical cord (Wharton's jelly [WJ]) and compared their capacity regarding differentiation toward female germ cells under influence of 10 ng/mL BMP4...
February 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28093199/effect-of-different-cryo-devices-on-in%C3%A2-vitro-maturation-and-development-of-vitrified-warmed-immature-buffalo-oocytes
#5
Yelisetti Uma Mahesh, Henderson Rose Winnie Gibence, Sisinthy Shivaji, Brahmasani Sambasiva Rao
The aim of the study was to identify a cryo-device that would be best suited for the vitrification of buffalo immature cumulus-oocyte complexes (COCs) as judged by viability and meiotic competence of the vitrified-warmed oocytes and their development ability following in vitro fertilization (IVF). The expression of oocyte secreting factors and their receptors (GDF9, BMP15, BMPR2, TGFBR1) and apoptosis related genes (BCL2, BAX, P53, C-MYC) were compared in vitrified-warmed oocytes after in vitro maturation...
January 16, 2017: Cryobiology
https://www.readbyqxmd.com/read/28087314/a-protective-role-of-cumulus-cells-after-short-term-exposure-of-rat-cumulus-cell-oocyte-complexes-to-lifestyle-or-environmental-contaminants
#6
Kelly A Campen, Kenneth P McNatty, Janet L Pitman
Ovarian follicular fluid provides a potential reservoir for exogenous compounds that may adversely affect oocyte quality. This study examined the effects of common lifestyle and environmental contaminants, namely bisphenol-A (BPA), caffeine, 3,4-methylenedioxymethamphetamine (MDMA), nicotine and Δ(9)-tetrahydrocannabinol (THC) on gap junction genes (Gja1, Gja4) and proteins (GJA1), glucose metabolism genes (Gfpt1, Pfkp) and oocyte growth factor genes (Bmp15, Gdf9), as well as gap junction transfer rate, in rat cumulus-oocyte complexes (COCs)...
January 10, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28075701/expression-analysis-of-genes-associated-with-prolificacy-in-fecb-carrier-and-noncarrier-indian-sheep
#7
S Goyal, J Aggarwal, P K Dubey, B P Mishra, P Ghalsasi, C Nimbkar, B K Joshi, R S Kataria
The effect of FecB mutation on the gene expression in FecB carrier and noncarrier estrous synchronized ewes, has been analyzed. For this study the whole ovarian tissues and Graafian follicles were collected from estrus synchronized FecB carrier Garole, and non-carrier Deccani Indian sheep, showing remarkable differences in the numbers of preovulatory follicles among two groups. Eleven potential candidate genes (BMP15, GDF9, BMP4, BMP7, BMPR1B, BMPR1A, SMAD9, LHCGR, FSHR, IGF1R, and STAT5) were selected for their expression analysis by SybrGreen based real-time PCR, across ovaries and Graafian follicles of different fecundity groups, for having better insights into the effect of FecB genotypes on follicular development...
January 11, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28072828/ggpp-mediated-protein-geranylgeranylation-in-oocyte-is-essential-for-the-establishment-of-oocyte-granulosa-cell-communication-and-primary-secondary-follicle-transition-in-mouse-ovary
#8
Chen Jiang, Fan Diao, Yong-Juan Sang, Na Xu, Rui-Lou Zhu, Xiu-Xing Wang, Zhong Chen, Wei-Wei Tao, Bing Yao, Hai-Xiang Sun, Xing-Xu Huang, Bin Xue, Chao-Jun Li
Folliculogenesis is a progressive and highly regulated process, which is essential to provide ova for later reproductive life, requires the bidirectional communication between the oocyte and granulosa cells. This physical connection-mediated communication conveys not only the signals from the oocyte to granulosa cells that regulate their proliferation but also metabolites from the granulosa cells to the oocyte for biosynthesis. However, the underlying mechanism of establishing this communication is largely unknown...
January 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28069901/bmp15-regulates-the-inhibin-activin-system-independently-of-ovulation-rate-control-in-sheep
#9
Anthony Estienne, Belén Lahoz, Peggy Jarrier, Loys Bodin, José Folch, José Luis Alabart, Stephane Fabre, Danielle Monniaux
Polymorphisms in the gene encoding bone morphogenetic protein 15 (BMP15) have been associated with multiple ovulations in sheep. As BMP15 regulates inhibin expression in rodents, we assumed that the ovarian inhibin/activin system could mediate part of the effect of BMP15 mutations in the regulation of ovulation rate in sheep. To answer this question, we have studied the effects of two natural loss-of-function mutations of BMP15 on the expression of components of this system. The FecX(R) and the FecX(Gr) mutations, when present respectively in Rasa Aragonesa ewes at the heterozygous state and in Grivette ewes at the homozygous state, were associated with a two-fold increase in ovulation rate...
January 9, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28040471/developmental-effects-of-imatinib-mesylate-on-follicle-assembly-and-early-activation-of-primordial-follicle-pool-in-postnatal-rat-ovary
#10
Babak Asadi-Azarbaijani, Regiane R Santos, Kirsi Jahnukainen, Saskia Braber, Majorie B M van Buursen, Jorma Toppari, Ola D Saugstad, Mirja Nurmio, Irma C Oskam
Imatinib mesylate is an anti-cancer agent that competitively inhibits several receptor tyrosine kinases (RTKs). RTKs play important roles in the regulation of primordial follicle formation, the recruitment of primordial follicles into the pool of growing follicles and maturation of the follicles. In the present study, we investigated the effects of the tyrosine kinase inhibitor imatinib on primordial follicle assembly and early folliculogenesis in postnatal rats. Female Sprague-Dawley rats were treated with either imatinib (150mg/kg) or placebo (water) on postnatal days 2-4...
December 28, 2016: Reproductive Biology
https://www.readbyqxmd.com/read/28039065/microrna-224-delays-oocyte-maturation-through-targeting-ptx3-in-cumulus-cells
#11
Xiufang Li, Huidan Wang, Yan Sheng, Zhongqing Wang
MicroRNAs (miRNAs) have been improved to regulate oocyte development in a cell- or stage-specific manner. In this study, we aimed to clarify microRNA-224's (miR-224) role in cumulus cells (CCs), to find out whether a change level of miR-224 in CCs could influence the maturation of oocyte. We found that overexpression of miR-224 of CCs led to the impairment of cell expansion, along with a decrease in the gene expression associated with cell expansion and maturation of oocyte. The increased expression of miR-224 in CC interrupted oocyte cell cycle at the GV stage...
December 28, 2016: Mechanisms of Development
https://www.readbyqxmd.com/read/27965086/the-effect-of-poly-adp-ribosyl-ation-inhibition-on-the-porcine-cumulus-oocyte-complex-during-in%C3%A2-vitro-maturation
#12
Duk Hyoun Kim, Hye Ran Lee, Min Gyeong Kim, Jun Sung Lee, Su Jin Jin, Hoon Taek Lee
Poly(ADP-ribosyl)ation (PARylation) plays important roles in DNA repair, apoptosis, transcriptional regulation, and cell death, and occurs via the activity of poly(ADP-ribose) polymerases (PARPs). Previous studies have shown that PARylation affects mouse and porcine pre-implantation development and participates in mechanisms of autophagy. However, there have not yet been reported the role of PARylation during in vitro maturation (IVM) of porcine oocytes. Thus, we investigated the effect of PARylation inhibition on this process; cumulus-oocyte complexes (COCs) were cultured with 3-aminobenzamide (3-ABA, PARP inhibitor) during porcine IVM...
January 29, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27943152/oocyte-maturation-embryo-development-and-gene-expression-following-two-different-methods-of-bovine-cumulus-oocyte-complexes-vitrification
#13
Mehdi Azari, Mojtaba Kafi, Bita Ebrahimi, Roya Fatehi, Mahboobeh Jamalzadeh
OBJECTIVE: To examine the maturational competence, embryo development and expression of genes involved in oocyte maturation and cumulus expansion (GDF9, BMP15, HAS2, TNFAIP6, FGF17 and FSHr) following two standard methods of bovine COCs vitrification. METHODS: Bovine cumulus-oocyte complexes (COCs) were aspirated from slaughtered ovaries and then distributed into three groups: non-vitrified COCs (control), vitrification 1 group (V1); vitrification was performed by 15% ethylene glycol (EG) and 15% DMSO in holding media (TCM-199 with 20% FCS); and vitrification 2 group (V2); vitrification was performed by 40% EG in holding media...
March 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/27930667/protein-phosphatase-6-protects-prophase-i-arrested-oocytes-by-safeguarding-genomic-integrity
#14
Meng-Wen Hu, Tie-Gang Meng, Zong-Zhe Jiang, Ming-Zhe Dong, Heide Schatten, Xingzhi Xu, Zhen-Bo Wang, Qing-Yuan Sun
Mammalian oocytes are arrested at prophase of the first meiotic division in the primordial follicle pool for months, even years, after birth depending on species, and only a limited number of oocytes resume meiosis, complete maturation, and ovulate with each reproductive cycle. We recently reported that protein phosphatase 6 (PP6), a member of the PP2A-like subfamily, which accounts for cellular serine/threonine phosphatase activity, functions in completing the second meiosis. Here, we generated mutant mice with a specific deletion of Ppp6c in oocytes from the primordial follicle stage by crossing Ppp6cF/F mice with Gdf9-Cre mice and found that Ppp6cF/F; GCre+ mice are infertile...
December 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27925309/bovine-ovarian-stem-cells-differentiate-into-germ-cells-and-oocyte-like-structures-after-culture-in-vitro
#15
G B de Souza, Jjn Costa, E V da Cunha, Jrs Passos, R P Ribeiro, Mva Saraiva, R van den Hurk, Jrv Silva
Stem cells have been isolated from ovaries, and their ability to differentiate into oocytes in vitro has been demonstrated for mice and human, but not for bovine species. The aims of this study were to isolate germline stem cells from bovine ovaries and to evaluate the effects of bone morphogenetic proteins (BMPs) 2 and 4, and follicular fluid on the differentiation of these stem cells into oocyte-like structures. The ovarian stem cells were isolated and cultured in α-MEM(+) supplemented with BMP2, BMP4 or follicular fluid...
December 7, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27866059/glucocorticoid-metabolism-in-equine-follicles-and-oocytes
#16
D Scarlet, N Ille, R Ertl, B G Alves, G D A Gastal, S O Paiva, M O Gastal, E L Gastal, C Aurich
The objective of this study was to determine whether (1) systemic and intrafollicular cortisol concentrations in horses are directly related and (2) supraphysiological levels of glucocorticoids affect in vitro maturation (IVM) rates of oocytes. Specifically, we studied the (1) changes in the intrafollicular cortisol and progesterone in context with granulosa cell gene expression during maturation of equine follicles (from 5-9 mm, 10-14 mm, 15-19 mm, 20-24 mm, and ≥25 mm in diameter) and (2) effects of cortisol supplementation on IVM rates and gene expression of equine cumulus-oocyte complexes (COCs)...
October 29, 2016: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/27862569/fibroblast-growth-factor-10-markedly-improves-in-vitro-maturation-of-porcine-cumulus-oocyte-complexes
#17
Yeo-Jin Son, Seung-Eun Lee, Hyuk Hyun, Min-Young Shin, Yun-Gwi Park, Sang-Gi Jeong, Eun-Young Kim, Se-Pill Park
Growth factors synthesized by ovarian somatic cells affect cumulus cell expansion and oocyte maturation in vitro. Fibroblast growth factor 10 (FGF10), for example, is a known regulator of mammalian cumulus-oocyte complex maturation. In this study, we investigated the effects of 0, 5, 10, 50, and 100 ng/mL FGF10 (5F, 10F, 50F, and 100F, respectively) on in vitro cumulus cell expansion, oocyte maturation, and embryo development. The percentage of fully expanded cumulus cells at the oocyte's metaphase-II (MII) stage was significantly higher in the 10F-treated group than in the control...
November 11, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27852727/ovarian-tissue-culture-in-the-presence-of-vegf-and-fetuin-stimulates-follicle-growth-and-steroidogenesis
#18
Ebrahim Asadi, Atefeh Najafi, Ashraf Moeini, Reihaneh Pirjani, Gholamreza Hassanzadeh, Saideh Mikaeili, Ensieh Salehi, Emmanuel Adutwum, Mansoureh Soleimani, Fariba Khosravi, Mahmood Barati, Farid Abolhassani
Ovarian tissue cryopreservation together with follicle culture provides a promising technique for fertility preservation in cancer patients. The study aimed to evaluate follicle parameters in a culture medium supplemented with VEGFA165 and/or fetuin. Vitrified-warmed ovarian cortical pieces were divided randomly into four culture groups consisting of basic culture medium (control), and the basic culture medium supplemented with VEGFA165, fetuin or both. After six days of culture, we evaluated the following: percentage of resting, primary and secondary growing follicles; survival rate; steroid hormones production; levels of reactive oxygen species, lipid peroxidation and total antioxidant capacity; and developmental and antioxidant gene expression...
February 2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/27836978/a-homozygous-nobox-truncating-variant-causes-defective-transcriptional-activation-and-leads-to-primary-ovarian-insufficiency
#19
Lin Li, Binbin Wang, Wei Zhang, Beili Chen, Minna Luo, Jing Wang, Xi Wang, Yunxia Cao, Kehkooi Kee
STUDY QUESTION: Does a novel homozygous NOBOX truncating variant, identified in whole exome sequencing (WES) of patients with primary ovarian insufficiency (POI), cause defective transcriptional activation of multiple oocyte-related genes? SUMMARY ANSWER: A novel homozygous truncating mutation of NOBOX was confirmed to exhibit a loss-of-function effect using well-defined molecular and functional analyses. WHAT IS KNOWN ALREADY: Several NOBOX mutations have been reported to be associated with POI but all of them are heterozygous mutations...
January 2017: Human Reproduction
https://www.readbyqxmd.com/read/27803898/candidate-gene-expression-in-bos-indicus-ovarian-tissues-prepubertal-and-postpubertal-heifers-in-diestrus
#20
Mayara Morena Del Cambre Amaral Weller, Marina Rufino S Fortes, Laercio R Porto-Neto, Matthew Kelly, Bronwyn Venus, Lisa Kidd, João Paulo Arcelino do Rego, Sophia Edwards, Gry B Boe-Hansen, Emily Piper, Sigrid A Lehnert, Simone Eliza Facioni Guimarães, Stephen Stewart Moore
Growth factors such as bone morphogenetic proteins 6, 7, 15, and two isoforms of transforming growth factor-beta (BMP6, BMP7, BMP15, TGFB1, and TGFB2), and insulin-like growth factor system act as local regulators of ovarian follicular development. To elucidate if these factors as well as others candidate genes, such as estrogen receptor 1 (ESR1), growth differentiation factor 9 (GDF9), follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), bone morphogenetic protein receptor, type 2 (BMPR2), type 1 insulin-like growth factor receptor (IGFR1), and key steroidogenic enzymes cytochrome P450 aromatase and 3-β-hydroxysteroid dehydrogenase (CYP19A1 and HSD3B1) could modulate or influence diestrus on the onset of puberty in Brahman heifers, their ovarian mRNA expression was measured before and after puberty (luteal phase)...
2016: Frontiers in Veterinary Science
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