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https://www.readbyqxmd.com/read/29331831/effect-of-bone-morphogenetic-proteins-2-and-4-on-survival-and-development-of-bovine-secondary-follicles-cultured-in%C3%A2-vitro
#1
E V da Cunha, L R F Melo, G B Sousa, V R Araújo, G L Vasconcelos, A W B Silva, J R V Silva
This study evaluated the effect of bone morphogenetic proteins 2 (BMP2) and 4 (BMP2) on follicle development and mRNA expression for GDF9, Cyclin B1, BMPR1A, BMPR1B, BMPRII, FSHR and SMAD1 in bovine secondary follicles cultured in vitro. Isolated secondary follicles were cultured for 18 days in TCM199+ medium alone or supplemented with BMP2 (10 ng/mL), BMP4 (100 ng/mL) or combination of both BMP2 and 4. Real-time PCR was used to analyze mRNA levels in fresh and cultured follicles. After 18 days of culture, follicles cultured with BMP2 alone or with BMP4 alone had larger diameters when compared to control (P < ...
December 27, 2017: Theriogenology
https://www.readbyqxmd.com/read/29275294/effects-of-2-3-bis-4-hydroxyphenyl-propionitrile-on-induction-of-polyovular-follicles-in-the-mouse-ovary
#2
Tomomi Sato, Hannah Kim, Hanako Kakuta, Taisen Iguchi
BACKGROUND/AIM: Neonatal diethylstilbestrol (DES) treatment induces polyovular follicles (PFs), which contain more than two oocytes in a follicle, through estrogen receptor (ER) β, not ERα. 2,3-Bis(4-hydroxyphenyl)-propionitrile (DPN) is a specific ERβ agonist; the effects of neonatal DPN exposure on PF induction and gene expression in the mouse ovary were examined. MATERIALS AND METHODS: Histological analysis and real-time reverse transcription-polymerase chain reaction were performed...
January 2018: In Vivo
https://www.readbyqxmd.com/read/29274749/the-genetic-polymorphisms-of-tgf%C3%AE-superfamily-genes-are-associated-with-litter-size-in-a-chinese-indigenous-sheep-breed-hu-sheep
#3
Weimin Wang, Yongfu La, Xiang Zhou, Xiaoxue Zhang, Fadi Li, Bang Liu
Litter size, which is a complex economic trait in sheep, is affected by polygenes. Hu sheep are a prolific native breed in China and also an ideal resource for studying the genetic mechanism that controls litter size in sheep. In this study, we investigated the genetic effects of candidate genes on litter size in a large experimental population (n = 2021) of Hu sheep. A total of 23 single nucleotide polymorphisms (SNPs) including six reported major mutations (FecB, FecXI, FecXB, FecXH, FecGI, and FecGH) and 17 novel SNPs in 10 candidate genes involved in reproduction were genotyped using KASPar technology...
December 16, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29233618/nutritional-impact-on-gene-expression-and-competence-of-oocytes-used-to-support-embryo-development-and-livebirth-by-cloning-procedures-in-goats
#4
C C L Fernandes, L H Aguiar, C E M Calderón, A M Silva, J P M Alves, R Rossetto, L R Bertolini, M Bertolini, D Rondina
Changes in the nutritional plan have been shown to affect oocyte quality, crucial to oocyte donors animals used in cloning. This study aimed to evaluate the impact of diets with increasing nutritional levels (maintenance diet=M; 1.3M; 1.6M; 1.9M) fed to goats for four weeks on follicular fluid composition, gene expression and oocyte competence used to cloning in goats. Donor females were superovulated for the retrieval of matured oocytes and physical measurements reported. After four weeks, groups receiving diets above maintenance increased thickness of subcutaneous adipose tissue and body weight, with higher values in 1...
October 19, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29221447/disruption-of-postnatal-folliculogenesis-and-development-of-ovarian-tumor-in-a-mouse-model-with-aberrant-transforming-growth-factor-beta-signaling
#5
Yang Gao, Xin Fang, David F Vincent, David W Threadgill, Laurent Bartholin, Qinglei Li
BACKGROUND: Transforming growth factor beta (TGFB) superfamily signaling is implicated in the development of sex cord-stromal tumors, a category of poorly defined gonadal tumors. The aim of this study was to determine potential effects of dysregulated TGFB signaling in the ovary using Cre recombinase driven by growth differentiation factor 9 (Gdf9) promoter known to be expressed in oocytes. METHODS: A mouse model containing constitutively active TGFBR1 (TGFBR1CA) using Gdf9-iCre (termed TGFBR1-CAG9Cre) was generated...
December 8, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29219221/improved-developmental-competence-in-embryos-treated-with-lycopene-during-in-vitro-culture
#6
M M R Chowdhury, Ayman Mesalam, Imran Khan, Myeong-Don Joo, Kyeong-Lim Lee, Lianguang Xu, Fahmida Afrin, Il-Keun Kong
In vitro embryo development remains suboptimal compared to in vivo development due to the challenge from various stressors associated with in vitro culturing of oocytes. When 0.2 μM lycopene was added to oocyte in vitro maturation and embryo culture media, to assess its antioxidant effects on embryo development, we observed a significant (p<0.05) increase in cleavage and blastocyst development rates compared to the corresponding controls (84.3 ± 0.6% vs. 73.1 ± 1.9% and 41.0 ± 1.4% vs. 33.4 ± 0.7%, respectively)...
December 8, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29177034/maximum-likelihood-approaches-reveal-signatures-of-positive-selection-in-bmp15-and-gdf9-genes-modulating-ovarian-function-in-mammalian-female-fertility
#7
Hafiz Ishfaq Ahmad, Guiqiong Liu, Xunping Jiang, Shishay Girmay Edallew, Teketay Wassie, Birhanu Tesema, Yu Yun, Liu Pan, Chenhui Liu, Yuqing Chong, Zhao Jia Yu, Han Jilong
Bone morphogenetic proteins (BMPs) and the growth factors (GDFs) play an important role in ovarian folliculogenesis and essential regulator of processes of numerous granulosa cells. BMP15 gene variations linked to various ovarian phenotypic consequences subject to the species, from infertility to improved prolificacy in sheep, primary ovarian insufficiency in women or associated with minor subfertility in mouse. To study the evolving role of BMP15 and GDF9, a phylogenetic analysis was performed. To find out the candidate gene associated with prolificacy in mammals, the nucleotide sequence of BMP15 and GDF9 genes was recognized under positive selection in various mammalian species...
November 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/29164452/identification-of-suh-gene-and-evidence-for-involvement-of-notch-signaling-pathway-on-gonadal-differentiation-of-yellow-river-carp-cyprinus-carpio
#8
Yongfang Jia, Fang Wang, Ruihua Zhang, Tingting Liang, WanWan Zhang, Xiaolin Ji, Qiyan Du, Zhongjie Chang
The suh gene is crucial in Notch pathway and regulates mammalian gonad development. In this study, the sequences of suh1 and suh2 genes in Yellow River carp (Cyprinus carpio) were verified. The partial 5'-flanking regions of suh1 and suh2 were analyzed and several potential transcription factor-binding sites were identified. Phylogenetic, gene structure, and chromosome synteny analyses revealed that carp suh1 and suh2 were orthologs and homologous to vertebrate suh. Investigation of the expression profiles of suh1 and suh2 with qPCR showed that these genes were abundant in the brain and gonad of carp, with suh1 exhibiting sexual dimorphism expression pattern in gonad...
November 21, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29157197/selection-for-female-traits-of-high-fertility-affects-male-reproductive-performance-and-alters-the-testicular-transcriptional-profile
#9
Marten Michaelis, Alexander Sobczak, Dirk Koczan, Martina Langhammer, Norbert Reinsch, Jennifer Schoen, Joachim M Weitzel
BACKGROUND: Many genes important for reproductive performance are shared by both sexes. However, fecundity indices are primarily based on female parameters such as litter size. We examined a fertility mouse line (FL2), which has a considerably increased number of offspring and a total litter weight of 180% compared to a randomly bred control line (Ctrl) after more than 170 generations of breeding. In the present study, we investigated whether there might be a parallel evolution in males after more than 40 years of breeding in this outbred mouse model...
November 21, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29098533/follicle-activation-is-a-significant-and-immediate-cause-of-follicle-loss-after-ovarian-tissue-transplantation
#10
Zohar Gavish, Itay Spector, Gil Peer, Stefan Schlatt, Joachim Wistuba, Hadassa Roness, Dror Meirow
PURPOSE: Extensive follicle loss has been demonstrated in ovarian grafts post transplantation, reducing their productivity and lifespan. Several mechanisms for this loss have been proposed, and this study aims to clarify when and how the massive follicle loss associated with transplantation of ovarian tissue graft occurs. An understanding of the mechanisms of follicle loss will pinpoint potential new targets for optimization and improvement of this important fertility preservation technique...
November 3, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29073214/robust-gdf9-and-bmp15-expression-in-the-oocytes-of-ovotestes-through-the-figla-independent-pathway-in-the-hermaphroditic-black-porgy-acanthopagrus-schlegelii
#11
Guan-Chung Wu, Jia-Wun Luo, Hau-Wen Li, Chen-Hsiu Huang, Ching-Fong Chang
More than 1,500 fish species are hermaphroditic, but no hermaphroditic lineage appears to be evolutionarily ancient in fishes. Thus, whether more than one sex at a time was present during the evolutionary shift from gonochorism to hermaphroditism in fishes is an intriguing question. Ectopic oocytes were created in the ovotestes of protandrous black porgy via the withdrawal of estradiol (E2) administration. These ectopic oocytes reprogrammed the surrounding cells, which changed from Sertoli cells to follicle-like cells...
2017: PloS One
https://www.readbyqxmd.com/read/29058487/lc3-dependent-autophagy-in-pig-2-cell-cloned-embryos-could-influence-the-degradation-of-maternal-mrna-and-the-regulation-of-epigenetic-modification
#12
Daming Chi, Yaqiong Zeng, Mingzhu Xu, Linan Si, Xiao Qu, Honglin Liu, Juan Li
In this study, the distribution as well as the effect of autophagy on reprogramming in pig cloned embryos were observed immediately after somatic cell nuclear transfer. Results showed that the LC3 was at the highest level in cloned embryos at 2-cell stage, and it decreased with the development from 2-cell stage to blastocyst. Different to cloned embryos, the intensity of LC3 in parthenogenetic activation (PA) embryos was at the highest level at 4-cell stage. A markedly higher level of Bmp15, H1foo, and Dppa3 was shown in cloned embryos at 2-cell stage (p < 0...
October 23, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/29049922/effect-of-co-culture-canine-cumulus-and-oviduct-cells-with-porcine-oocytes-during-maturation-and-subsequent-embryo-development-of-parthenotes-in%C3%A2-vitro
#13
Seok Hee Lee, Hyun Ju Oh, Min Jung Kim, Geon A Kim, Yoo Bin Choi, Young Kwang Jo, Erif Maha Nugraha Setyawan, Byeong Chun Lee
In the estrus stage, canine oocytes are surrounded by cumulus cells and undergo maturation in the oviduct for 2-3 days after ovulation. We hypothesized that canine oviduct cells (cOC) and canine cumulus cells (cCC) during this stage might affect the maturation of oocytes and thereby improve subsequent embryo development. Therefore, the objective of this study was to compare the effects of a cOC and cCC co-culture on oocyte in vitro maturation (IVM) and subsequent embryo development, and to analyze the gene expressions in a molecular fashion what co-culture actually gives the specific pathways in which the co-culture cells act to improve maturation and embryo development...
October 3, 2017: Theriogenology
https://www.readbyqxmd.com/read/29047400/observation-of-the-influences-of-diosgenin-on-aging-ovarian-reserve-and-function-in-a-mouse-model
#14
Mingjie Shen, Cong Qi, Yan-Ping Kuang, Yang Yang, Qi-Feng Lyu, Hui Long, Zhi-Guang Yan, Ying-Yu Lu
BACKGROUND: The aim of this study was to investigate the impact of diosgenin, an important monomer of sapogenins in yams, on ovarian reserve in a natural aging mice model. STUDY DESIGN: This randomized controlled trial included 60 9-month-old C57 naturally aging female mice. Twenty-one mice were assigned to the dio group and were fed a single dose of diosgenin (200 mg/kg/day) suspended in 0.3% CMC. Twenty mice were assigned to the DHEA group and were fed a single dose of DHEA (1...
October 18, 2017: European Journal of Medical Research
https://www.readbyqxmd.com/read/29044499/identification-of-the-first-homozygous-1-bp-deletion-in-gdf9-gene-leading-to-primary-ovarian-insufficiency-by-using-targeted-massively-parallel-sequencing
#15
M M França, M F A Funari, M Y Nishi, A M Narcizo, S Domenice, E M F Costa, A M Lerario, B B Mendonca
Targeted massively parallel sequencing (TMPS) has been used in genetic diagnosis for Mendelian disorders. In the past few years, the TMPS has identified new and already described genes associated with primary ovarian insufficiency (POI) phenotype. Here, we performed a targeted gene sequencing to find a genetic diagnosis in idiopathic cases of Brazilian POI cohort. A custom SureSelectXT DNA target enrichment panel was designed and the sequencing was performed on Illumina NextSeq sequencer. We identified 1 homozygous 1-bp deletion variant (c...
October 16, 2017: Clinical Genetics
https://www.readbyqxmd.com/read/29043702/n-acetylcysteine-compared-to-metformin-improves-the-expression-profile-of-growth-differentiation-factor-9-and-receptor-tyrosine-kinase-c-kit-in-the-oocytes-of-patients-with-polycystic-ovarian-syndrome
#16
Ebrahim Cheraghi, Malek Soleimani Mehranjani, Seyed Mohammad Ali Shariatzadeh, Mohammad Hossein Nasr Esfahani, Behrang Alani
Background: Paracrine disruption of growth factors in women with polycystic ovarian syndrome (PCOS) results in production of low quality oocyte, especially following ovulation induction. The aim of this study was to investigate the effects of metformin (MET), N-acetylcysteine (NAC) and their combination on the hormonal levels and expression profile of GDF-9, BMP-15 and c-kit, as hallmarks of oocyte quality, in PCOS patients. MATERIALS AND METHODS: This prospective randomized, double-blind, placebo controlled trial aims to study the effects of MET, NAC and their combination (MET+NAC) on expression of GDF-9, BMP-15 and c-kit mRNA in oocytes [10 at the germinal vesicle (GV) stage, 10 at the MI stage, and 10 at the MII stage from per group] derived following ovulation induction in PCOS...
January 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29040417/neonatal-immune-activation-depletes-the-ovarian-follicle-reserve-and-alters-ovarian-acute-inflammatory-mediators-in-neonatal-rats
#17
Erin A Fuller, Luba Sominsky, Jessie M Sutherland, Kate A Redgrove, Lauren Harms, Eileen A McLaughlin, Deborah M Hodgson
Normal ovarian development is crucial for female reproductive success and longevity. Interruptions to the delicate process of initial folliculogenesis may lead to ovarian dysfunction. We have previously demonstrated that an early life immune challenge in the rat, induced by administration of lipopolysaccharide (LPS) on postnatal day (PND) 3 and 5, depletes ovarian follicle reserve long term. Here, we hypothesised that this neonatal immune challenge leads to an increase in peripheral and ovarian inflammatory signalling, contributing to an acute depletion of ovarian follicles...
October 10, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29028960/ovarian-follicular-theca-cell-recruitment-differentiation-and-impact-on-fertility-2017-update
#18
JoAnne S Richards, Yi A Ren, Nicholes Candelaria, Jaye E Adams, Aleksandar Rajkovic
The major goal of this review is to summarize recent exciting findings that have been published within the past 10 years that, to our knowledge, have not been presented in detail in previous reviews and that may impact altered follicular development in polycystic ovarian syndrome (PCOS) and premature ovarian failure (POF) in women. Specifically, we will cover 1) mouse models that have led to discovery of the derivation of two precursor populations of theca cells in the embryonic gonad, 2) the key roles of the oocyte-derived factor GDF9 on the hedgehog (HH) signaling pathway and theca cell functions and 3) the impact of the HH pathway on both the specification of theca endocrine cells and theca fibroblast and smooth muscle cells in developing follicles...
October 5, 2017: Endocrine Reviews
https://www.readbyqxmd.com/read/29023802/a-novel-12-bp-indel-polymorphism-within-the-gdf9-gene-is-significantly-associated-with-litter-size-and-growth-traits-in-goats
#19
Xinyu Wang, Qing Yang, Ke Wang, Sihuan Zhang, Chuanying Pan, Hong Chen, Lei Qu, Hailong Yan, Xianyong Lan
No abstract text is available yet for this article.
October 11, 2017: Animal Genetics
https://www.readbyqxmd.com/read/28986745/nutritional-and-metabolic-stressors-on-ovine-oocyte-development-and-granulosa-cell-functions-in-vitro
#20
S Nandi, S K Tripathi, P S P Gupta, S Mondal
The present study was undertaken to study the effect of ammonia, urea, non-esterified fatty acid (NEFA), and β-hydroxybutyric acid (β-OHB) on oocyte development and granulosa cell (GC) growth parameter of ovine (Ovis aries). Ovine oocytes were matured in vitro in the presence of different concentration of ammonia, urea, NEFA, and β-OHB for 24 h, in vitro inseminated and evaluated for cleavage and blastocyst yield. Same concentrations of ammonia, urea, NEFA, and β-OHB were examined on growth parameters and hormone secretion activity of granulosa cells in vitro...
October 6, 2017: Cell Stress & Chaperones
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