keyword
MENU ▼
Read by QxMD icon Read
search

carbonylated proteins

keyword
https://www.readbyqxmd.com/read/29160372/an-experimental-study-of-ascorbic-acid-effects-in-acute-renal-failure-under-general-anesthesia
#1
Omur Ozturk, Sefer Ustebay, Huseyin Avni Eroglu, Murat Günay, Yasemen Adali, İlksen Donmez, Mesut Erbas
PURPOSE: To evaluate the preventive effect of ascorbic acid on sevoflurane-induced acute renal failure in an experimental rat model. METHODS: Twenty-four adult male Wistar rats were randomly distributed into three groups. Subjects were allocated into 3 groups: Group I received sevoflurane only, whereas Groups II and III had moderate (150 mg/kg) and high (300 mg/kg) doses of AA in addition to sevoflurane, respectively. Rhabdomyolysis and myohemoglobinuric ARF was formed by intramuscular administration of glycerol on the upper hind limb on the 15th minute of inhalation anesthesia...
October 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29158541/dysregulation-of-mitochondrial-function-and-biogenesis-modulators-in-adipose-tissue-of-obese-children
#2
R Zamora-Mendoza, H Rosas-Vargas, M T Ramos-Cervantes, P Garcia-Zuniga, H Perez-Lorenzana, P Mendoza-Lorenzo, A Perez-Ortiz, J Estrada, A Miliar-Garcia, E Lara-Padilla, G Ceballos, A Rodriguez, F Villarreal, I Ramirez-Sanchez
BACKGROUND/OBJECTIVES: We aimed to evaluate mitochondrial biogenesis (MB), structure, metabolism, and dysfunction in abdominal adipose tissue from male pediatric patients with obesity. SUBJECTS/METHODS: Samples were collected from five children with obesity (percentile ⩾95) and five eutrophic boys (percentile ⩾5/⩽85) (8-12 years old) following parental informed consent. We analyzed the expression of key genes involved in MB (SIRT1, PPARγ, PGC1α, NRF1, NRF2, and TFAM) and surrogates for mitochondrial function/structure/metabolism (porin, TOMM20, complex I and V, UCP1, UCP2, SIRT3, SOD2) by Western blot...
November 21, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29158224/a-novel-fluorescent-reporter-detects-plastic-remodeling-of-mitochondria-er-contact-sites
#3
Zhaoying Yang, Xiaocui Zhao, Jiashen Xu, Weina Shang, Chao Tong
Mitochondria-ER contact sites (MERCs) enable communication between ER and mitochondria and serve as platforms for many cellular events, including autophagy. Nonetheless, the molecular organization of MERCs is not known, and there is no bona fide marker of these contact sites in mammalian cells. In this study, we designed a genetically encoded reporter using split super-folder GFP protein for labeling MERCs. We subsequently analyzed its distribution and dynamics during the cell cycle and under normal or stressful cellular conditions such as starvation, apoptosis, and ER stress...
November 20, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/29156622/advanced-glycation-end-products-in-the-pathogenesis-of-psoriasis
#4
REVIEW
Anastasia Papagrigoraki, Martina Maurelli, Micol Del Giglio, Paolo Gisondi, Giampiero Girolomoni
Advanced glycation end products (AGEs) are extremely oxidant and biologically reactive compounds, which form through oxidation of sugars, lipids and amino acids to create aldehydes that bind covalently to proteins. AGEs formation and accumulation in human tissues is a physiological process during ageing but it is enhanced in case of persistent hyperglycemia, hyperlipidemia and oxidative or carbonyl stress, which are common in patients with moderate to severe psoriasis. Exogenous AGEs may derive from foods, UV irradiation and cigarette smoking...
November 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29155085/ir-spectroscopy-analysis-of-pancreatic-lipase-related-protein-2-interaction-with-phospholipids-2-discriminative-recognition-of-various-micellar-systems-and-characterization-of-plrp2-dppc-bile-salt-complexes
#5
Eduardo Mateos-Diaz, Priscila Sutto-Ortiz, Moulay Sahaka, Deborah Byrne, Hélène Gaussier, Frédéric Carrière
The interaction of pancreatic lipase-related protein 2 (PLRP2) with various micelles containing phospholipids was investigated using pHstat enzyme activity measurements, differential light scattering, size exclusion chromatography (SEC) and transmission IR spectroscopy. Various micelles of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and lysophosphatidylcholine were prepared with either bile salts (sodium taurodeoxycholate or glycodeoxycholate) or Triton X-100, which are substrate-dispersing agents commonly used for measuring phospholipase activities...
November 16, 2017: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/29154839/participation-of-the-anti-inflammatory-and-antioxidative-activity-of-docosahexaenoic-acid-on-indomethacin-induced-gastric-injury-model
#6
Elizabeth Arlen Pineda-Peña, Yoalli Martínez-Pérez, Marina Galicia-Moreno, Araceli Navarrete, José Segovia, Pablo Muriel, Liliana Favari, Gilberto Castañeda-Hernández, Aracely Evangelina Chávez-Piña
Adverse gastrointestinal (GI) effects caused by nonsteroidal anti-inflammatory drugs (NSAIDs), including indomethacin, are recognized as the major limitation to their clinical use. NSAID-induced gastric damage is generated by cyclooxygenase inhibition, activation of inflammatory processes, and oxidative stress. Docosahexaenoic acid (DHA), an omega-3 polyunsaturated fatty acid, has shown gastroprotective effects; however, the molecular mechanisms underlying these effects have not been fully explained. As a result, the aim of this study was to examine DHA's anti-inflammatory and antioxidative actions in a mouse model of indomethacin-induced gastric injury...
November 14, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29154063/the-impact-of-silymarin-on-antioxidant-and-oxidative-status-in-patients-with-%C3%AE-thalassemia-major-a-crossover-randomized-controlled-trial
#7
Hadi Darvishi-Khezri, Ebrahim Salehifar, Mehrnoush Kosaryan, Hossein Karami, Abbas Alipour, Fatemeh Shaki, Aily Aliasgharian
BACKGROUND & AIMS: Blood transfusion therapy is lifesaving for individuals with β-thalassemia major (β-TM). Iron burden following blood transfusion is the main cause of oxidative stress (OS) and organ dysfunction in these patients. The aim of this study was to evaluate the effects of silymarin on serum antioxidant and oxidative status in patients with β-TM. METHODS: A crossover, randomized controlled trial was performed on 82 thalassemia patients. In two periods of 12 weeks, patients received 420mg silymarin (divided into three equal 140-mg daily doses) and placebo...
December 2017: Complementary Therapies in Medicine
https://www.readbyqxmd.com/read/29151266/crystal-structure-and-catalytic-characterization-of-the-dehydrogenase-reductase-sdr-family-member-4-dhrs4-from-caenorhabditis-elegans
#8
Michael Kisiela, Annette Faust, Bettina Ebert, Edmund Maser, Axel J Scheidig
The human dehydrogenase/reductase SDR family member 4 (DHRS4) is a tetrameric protein that is involved in the metabolism of several aromatic carbonyl compounds, steroids and bile acids. The only invertebrate DHRS4 that has been characterized to date is that from the model organism Caenorhabditis elegans. We have previously cloned and initially characterized this protein that was recently annotated as DHRS4_CAEEL in the UniProtKB database. Crystallization and X-ray diffraction studies of the full-length DHRS4_CAEEL protein in complex with diacetyl revealed its tetrameric structure and showed that two subunits are connected via an intermolecular disulfide bridge that is formed by N-terminal cysteine residues (Cys5) of each protein chain, which increases the enzymatic activity...
November 19, 2017: FEBS Journal
https://www.readbyqxmd.com/read/29148897/oxidative-stress-response-in-sh-sy5y-cells-exposed-to-short-term-1800%C3%A2-mhz-radiofrequency-radiation
#9
Ana Marija Marjanovic Cermak, Ivan Pavicic, Ivancica Trosic
The exact mechanism that could explain the effects of radiofrequency (RF) radiation exposure at non-thermal level is still unknown. Increasing evidence suggests a possible involvement of reactive oxygen species (ROS) and development of oxidative stress. To test the proposed hypothesis, human neuroblastoma cells (SH-SY5Y) were exposed to 1800 MHz short-term RF exposure for 10, 30 and 60 minutes. Electric field strength within Gigahertz Transverse Electromagnetic cell (GTEM) was 30 V m(-1) and specific absorption rate (SAR) was calculated to be 1...
November 17, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/29147907/protein-repair-from-glycation-by-glyoxals-by-the-dj-1-family-maillard-deglycases
#10
Mouadh Mihoub, Jad Abdallah, Gilbert Richarme
DJ-1 and its prokaryotic homologs, Hsp31, YhbO and YajL from Escherichia coli and PfpI from Pyrococcus furiosus, repair proteins from glycation by glyoxals (R-CO-CHO), which constitute their major glycating agents. Glycation is a non-enzymatic covalent reaction discovered by Louis Camille Maillard in 1912, between reactive carbonyls (reducing sugars and glyoxals) and amino acids (cysteine, arginine and lysine), which inactivates proteins. By degrading Maillard adducts formed between carbonyls and thiols or amino groups, the DJ-1 family Maillard deglycases prevent the formation of the so-called advanced glycation end products (AGEs) that arise from Maillard adducts after dehydrations, oxidations and rearrangements...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29146341/oxidation-and-nitrosation-of-meat-proteins-under-gastro-intestinal-conditions-consequences-in-terms-of-nutritional-and-health-values-of-meat
#11
Diane de La Pomélie, Véronique Santé-Lhoutellier, Thierry Sayd, Philippe Gatellier
The chemical changes (oxidation/nitrosation) of meat proteins during digestion lead to a decrease in their nutritional value. Moreover, oxidized and nitrosated amino acids are suspected to promote various human pathologies. To investigate the mechanisms and the kinetics of these endogenous protein modifications, we used a dynamic artificial digestive system (DIDGI®) that mimics the physicochemical conditions of digestion. The combined effect of meat cooking and endogenous addition of ascorbate and nitrite was evaluated on protein oxidation (by measuring carbonyl groups), protein nitrosation (by measuring nitrosamines), and proteolysis...
March 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29146333/comparative-time-course-of-lipid-and-myofibrillar-protein-oxidation-in-different-biphasic-systems-under-hydroxyl-radical-stress
#12
Jiayi Yang, Youling L Xiong
This study aimed to investigate the relative reaction rate of protein and lipid oxidation in different biphasic model systems (linoleic acid; liposome; emulsion) containing myofibrillar protein (MFP at 1, 8 and 20 mg/mL) under hydroxyl radical stress. Protein oxidation markers exhibited significant changes in 2 h: reduced tryptophan fluorescence intensity, carbonyl formation, and extensive polymerization of myosin. In contrast, no detectable changes (P > .05) in lipid peroxide occurred within 2 h except for samples with 1 mg/mL MFP which showed an early TBARS formation...
March 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29143122/vitamin-d-supplementation-attenuates-oxidative-stress-in-paraspinal-skeletal-muscles-in-patients-with-low-back-pain
#13
Katarzyna Dzik, Wojciech Skrobot, Damian Jozef Flis, Mateusz Karnia, Witold Libionka, Wojciech Kloc, Jan Jacek Kaczor
PURPOSE: The aim of the study was to evaluate markers of oxidative stress and vitamin D receptor in paraspinal muscles in low back pain patients with vitamin D deficiency, with normal level of vitamin D, and after 5 weeks of vitamin D supplementation. METHODS: Patients were divided into three groups: supplemented (SUP) with vitamin D, placebo with normal concentration of vitamin D (SUF), and the placebo group with vitamin D deficiency (DEF). The concentration of serum vitamin D was measured before and after the supplementation with vitamin D (3200 IU/ day for 5 weeks)...
November 15, 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/29140133/ite-inhibits-growth-of-human-pulmonary-artery-endothelial-cells
#14
Ling-Pin Pang, Yan Li, Qing-Yun Zou, Chi Zhou, Wei Lei, Jing Zheng, Shi-An Huang
AIM: Pulmonary arterial hypertension (PAH), a deadly disorder is associated with excessive growth of human pulmonary artery endothelial (HPAECs) and smooth muscle (HPASMCs) cells. Current therapies primarily aim at promoting vasodilation, which only ameliorates clinical symptoms without a cure. 2-(1'H-indole-3'-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE) is an endogenous aryl hydrocarbon receptor (AhR) ligand, and mediates many cellular function including cell growth. However, the roles of ITE in human lung endothelial cells remain elusive...
October 2017: Experimental Lung Research
https://www.readbyqxmd.com/read/29139577/copper-uptake-patterns-of-bioaccumulation-and-effects-in-glochidia-larvae-of-the-freshwater-mussel-lampsilis-cardium
#15
Marianna Basso Jorge, Adalto Bianchini, Chris M Wood, Patricia L Gillis
The early life stages of freshwater mussels are particularly sensitive to Cu contamination. We measured the acute toxicity, bioaccumulation and sublethal effects of Cu in glochidia. Additionally, we used radiolabeled Cu ((64) Cu) to examine the time-dependent kinetics of uptake over 24 h. (64) Cu uptake by live and dead glochidia exposed to 0.11 μmol/L exhibited similar hyperbolic patterns over the first 40 min, indicating an adsorptive phase independent of larval metabolism. Thereafter, uptake was linear with time, with a 10-fold lower bioaccumulation rate in live than in dead animals, representing a close to steady state of Cu regulation...
November 15, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/29138968/mitochondrial-dysfunctions-contribute-to-energy-deficits-in-rodent-model-of-hepatic-encephalopathy
#16
Saurabh Dhanda, Aditya Sunkaria, Avishek Halder, Rajat Sandhir
Perturbations in the cerebral energy metabolism are anticipated to be an important factor by which ammonia may exert its toxic effects on the central nervous system. The present study was designed to investigate the role of impaired mitochondrial functions and cerebral energy metabolism in the development hepatic encephalopathy (HE) induced by of bile duct ligation (BDL). After four weeks of BDL, a significant increase in hepatic hydroxyproline and collagen content was observed which confirmed biliary fibrosis...
November 14, 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/29138680/enhancement-of-antioxidant-mechanisms-and-reduction-of-oxidative-stress-in-chickens-after-the-administration-of-drinking-water-enriched-with-polyphenolic-powder-from-olive-mill-waste-waters
#17
Aliki Papadopoulou, Konstantinos Petrotos, Dimitrios Stagos, Konstantinos Gerasopoulos, Antonios Maimaris, Haralampos Makris, Ioannis Kafantaris, Sotiria Makri, Efthalia Kerasioti, Maria Halabalaki, Vincent Brieudes, Georgia Ntasi, Stylianos Kokkas, Pavlos Tzimas, Panagiotis Goulas, Alexander M Zakharenko, Kirill S Golokhvast, Aristidis Tsatsakis, Demetrios Kouretas
The aim of the study was to examine the effects of a polyphenolic powder from olive mill wastewater (OMWW) administered through drinking water, on chickens' redox status. Thus, 75 chickens were divided into three groups. Group A was given just drinking water, while groups B and C were given drinking water containing 20 and 50 μg/ml of polyphenols, respectively, for 45 days. The antioxidant effects of the polyphenolic powder were assessed by measuring oxidative stress biomarkers in blood after 25 and 45 days of treatment...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29135935/tucum-do-cerrado-bactris-setosa-mart-may-promote-anti-aging-effect-by-upregulating-sirt1-nrf2-pathway-and-attenuating-oxidative-stress-and-inflammation
#18
Marcela de Sá Barreto da Cunha, Sandra Fernandes Arruda
Aging may be related to oxidative damage accumulation and a low-grade inflammation, both responses are modulated by iron and phytochemicals. This study investigated the effect of tucum-do-cerrado (Bactris setosa Mart.) consumption on the expression of sirtuins (SIRT 1 and 3) and senescence marker protein-30 (SMP30), and on the redox and inflammatory responses, in adult rats supplemented or not with dietary iron. Male Wistar rats were treated for 12 weeks with: control diet (CT); iron enriched-diet (+Fe); control diet + 15% tucum-do-cerrado (Tuc); or iron enriched-diet + 15% tucum-do-cerrado (Tuc + Fe)...
November 14, 2017: Nutrients
https://www.readbyqxmd.com/read/29132320/histologic-chorioamnionitis-does-not-modulate-the-oxidative-stress-and-antioxidant-status-in-pregnancies-complicated-by-spontaneous-preterm-delivery
#19
Laura Fernandes Martin, Natália Prearo Moço, Moisés Diôgo de Lima, Jossimara Polettini, Hélio Amante Miot, Camila Renata Corrêa, Ramkumar Menon, Márcia Guimarães da Silva
BACKGROUND: Infection induced-inflammation and other risk factors for spontaneous preterm birth (PTB) and preterm premature rupture of membranes (pPROM) may cause a redox imbalance, increasing the release of free radicals and consuming antioxidant defenses. Oxidative stress, in turn, can initiate intracellular signaling cascades that increase the production of pro-inflammatory mediators. The objective of this study was to evaluate the oxidative damage to proteins and antioxidant capacity profiles in amniochorion membranes from preterm birth (PTB) and preterm premature rupture of membranes (pPROM) and to determine the role of histologic chorioamnionitis in this scenario...
November 13, 2017: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/29131581/template-assisted-enzymatic-synthesis-of-oligopeptides-from-a-polylactide-chain
#20
Jenny Fagerland, Daniela Pappalardo, Björn Schmidt, Per-Olof Syrén, Anna Finne Wistrand
Peptides are often attached to polymer materials, as bioactive components, for the control of interactions between the material and its surrounding proteins and cells. However, synthesizing peptides and attaching them to polymers can be challenging and laborious. Herein, we describe the grafting of oligopeptides to an aliphatic polyester, using a one-step chemo-enzymatic synthesis with papain as the biocatalyst. To enable enzyme-mediated functionalization of the polyester, ethyl hept-6-enoylalaninate (grafter) was synthesized and attached to polylactide chains using thiol-ene click reactions...
November 13, 2017: Biomacromolecules
keyword
keyword
36460
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"