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BMP-2 electrospun

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https://www.readbyqxmd.com/read/27319221/delivery-of-rhbmp-2-plasmid-dna-complexes-via-a-plla-collagen-electrospun-scaffold-induces-ectopic-bone-formation
#1
Xia Zhao, David E Komatsu, Michael Hadjiargyrou
The development of effective strategies for gene delivery is a critical goal in DNA-based tissue engineering. Previously, our laboratory utilized the process of electrospinning to fabricate plasmid DNA-based polymeric scaffolds. Although there lease of DNA was robust, the in vitro transfection efficiency was low. In order to optimize these results, we recently modified our approach and utilized a strategy to adsorb plasmid DNA transfection complexes onto a PLLA/Collagen I electrospun scaffold for the delivery of recombinant human Bone Morphogenetic Protein-2 (rhBMP-2)...
June 2016: Journal of Biomedical Nanotechnology
https://www.readbyqxmd.com/read/26642073/fabrication-of-multi-biofunctional-gelatin-based-electrospun-fibrous-scaffolds-for-enhancement-of-osteogenesis-of-mesenchymal-stem-cells
#2
Wei-Han Lin, Jiashing Yu, Guoping Chen, Wei-Bor Tsai
Biofunctional scaffolds that support the adhesion, proliferation, and osteo-differentiation of mesenchymal stem cells (MSCs) are critical for bone tissue engineering. In this study, a simple in situ UV-crosslinking strategy was utilized to fabricate gelatin electrospun fibrous (GEF) scaffolds with multiple biosignals, including cell adhesive Arg-Gly-Asp (RGD) peptide, osteo-conductive hydroxyapatite (HAp) nanoparticles, and osteo-inductive bone morphogenic protein-2 (BMP-2). The adhesion and proliferation of MSCs on the GEF scaffolds were improved by the incorporation of RGD...
February 1, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/26474622/-wound-healing-effects-of-silk-fibroin-bone-morphogenetic-protein-2-scaffolds-on-inflammatory-pulp-in-rats
#3
Tao Yu, Ting Jiang, Qing-mei Wei, Yi-fen Li, David L Kaplan
OBJECTIVE: To identify the healing effect of electrospun silk fibroin-BMP-2 as a biologic pulp capping agent to inflammatory pulp in rat caused by lipopolysaccharide (LPS). METHODS: A total of 30 healthy adult male Wistar rats were randomly divided into five groups:(1) normal control group without operation; (2) blank control group without capping agents; (3) calcium hydroxide capping group; (4) electrospun silk fibroin capping group; (5) electrospun silk fibroin-BMP-2 capping group...
October 18, 2015: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/26119373/a-nano-micro-alternating-multilayer-scaffold-loading-with-rbmscs-and-bmp-2-for-bone-tissue-engineering
#4
Shan Ding, Long Li, Xian Liu, Guang Yang, Guangliang Zhou, Shaobing Zhou
In this study, we develop a nano-micro alternating multilayer scaffold for bone tissue engineering by incorporation of monodispersed calcium alginate microbeads into electrospun polymer nanofibers. Both rat bone marrow mesenchymal stem cells (rBMSCs) and bone morphogenetic protein-2 (BMP-2) are simultaneously loaded into the microbeads, which are generated from a microfluidic device. The layer number of the scaffold can be readily controlled by alternately repeating the electrospinning and the microfluidic processes...
September 1, 2015: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/26106926/membrane-reinforced-three-dimensional-electrospun-silk-fibroin-scaffolds-for-bone-tissue-engineering
#5
Sung Yeun Yang, Tae Heon Hwang, Lihua Che, Jin Soo Oh, Yoon Ha, WonHyoung Ryu
Electrospun silk fibroin (SF) scaffolds have drawn much attention because of their resemblance to natural tissue architecture such as extracellular matrix, and the biocompatibility of SF as a candidate material to replace collagen. However, electrospun scaffolds lack the physical integrity of bone tissue scaffolds, which require resistance to mechanical loadings. In this work, we propose membrane-reinforced electrospun SF scaffolds by a serial process of electrospinning and freeze-drying of SF solutions in two different solvents: formic acid and water, respectively...
June 24, 2015: Biomedical Materials
https://www.readbyqxmd.com/read/26082632/mesoporous-bioactive-glass-surface-modified-poly-lactic-co-glycolic-acid-electrospun-fibrous-scaffold-for-bone-regeneration
#6
Shijie Chen, Zhiyuan Jian, Linsheng Huang, Wei Xu, Shaohua Liu, Dajiang Song, Zongmiao Wan, Amanda Vaughn, Ruisen Zhan, Chaoyue Zhang, Song Wu, Minghua Hu, Jinsong Li
A mesoporous bioactive glass (MBG) surface modified with poly(lactic-co-glycolic acid) (PLGA) electrospun fibrous scaffold for bone regeneration was prepared by dip-coating a PLGA electrospun fibrous scaffold into MBG precursor solution. Different surface structures and properties were acquired by different coating times. Surface morphology, chemical composition, microstructure, pore size distribution, and hydrophilicity of the PLGA-MBG scaffold were characterized. Results of scanning electron microscopy indicated that MBG surface coating made the scaffold rougher with the increase of MBG content...
2015: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/25823598/effects-of-immobilized-bmp-2-and-nanofiber-morphology-on-in-vitro-osteogenic-differentiation-of-hmscs-and-in-vivo-collagen-assembly-of-regenerated-bone
#7
Sajeesh Kumar Madhurakkat Perikamana, Jinkyu Lee, Taufiq Ahmad, Yonghoon Jeong, Do-Gyoon Kim, Kyobum Kim, Heungsoo Shin
Engineering bone tissue is particularly challenging because of the distinctive structural features of bone within a complex biochemical environment. In the present study, we fabricated poly(L-lactic acid) (PLLA) electrospun nanofibers with random and aligned morphology immobilized with bone morphogenic protein-2 (BMP-2) and investigated how these signals modulate (1) in vitro osteogenic differentiation of human mesenchymal stem cells (hMSCs) and (2) in vivo bone growth rate, mechanical properties, and collagen assembly of newly formed bone...
April 29, 2015: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/25813520/salicylic-acid-based-polymers-for-guided-bone-regeneration-using-bone-morphogenetic-protein-2
#8
Sangeeta Subramanian, Ashley Mitchell, Weiling Yu, Sabrina Snyder, Kathryn Uhrich, J Patrick O'Connor
Bone morphogenetic protein-2 (BMP-2) is used clinically to promote spinal fusion, treat complex tibia fractures, and to promote bone formation in craniomaxillofacial surgery. Excessive bone formation at sites where BMP-2 has been applied is an established complication and one that could be corrected by guided tissue regeneration methods. In this study, anti-inflammatory polymers containing salicylic acid [salicylic acid-based poly(anhydride-ester), SAPAE] were electrospun with polycaprolactone (PCL) to create thin flexible matrices for use as guided bone regeneration membranes...
July 2015: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/25689580/evaluation-of-nanostructure-and-microstructure-of-bone-regenerated-by-bmp-2-porous-scaffolds
#9
Carlos Del Rosario, Maria Rodríguez-Evora, Ricardo Reyes, Alejandro González-Orive, Alberto Hernández-Creus, Kevin M Shakesheff, Lisa J White, Araceli Delgado, Carmen Evora
In this study, three systems containing BMP-2 were fabricated, including two electrospun sandwich-like-systems of PLGA 75:25 and PLGA 50:50 and a microsphere system of PLGA 50:50 to be implanted in a critical size defect in rat calvaria. The in vivo BMP-2 release profiles of the three systems were similar. The total dose was released during the first two weeks. To evaluate the nano and microstructure of the regenerated bone a multi-technique analysis was used, including stereo microscope, X-Ray; AFM, micro-CT, and histological analyses...
September 2015: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/25453952/controlled-dual-delivery-of-bmp-2-and-dexamethasone-by-nanoparticle-embedded-electrospun-nanofibers-for-the-efficient-repair-of-critical-sized-rat-calvarial-defect
#10
Long Li, Guangliang Zhou, Yi Wang, Guang Yang, Shan Ding, Shaobing Zhou
There is an urgent need to develop biomimetic bone tissue engineering scaffolds for the repair of critical-sized calvarial defect. In this study, we developed a new nanoparticle-embedded electrospun nanofiber scaffold for the controlled dual delivery of BMP-2 and dexamethasone (DEX). The scaffold was achieved by (1) the encapsulation of BMP-2 into bovine serum albumin (BSA) nanoparticles to maintain the bioactivity of BMP-2 and (2) the co-electrospinning of the blending solution composed of the BSA nanoparticles, DEX and the poly(ε-caprolactone)-co-poly(ethylene glycol) (PCE) copolymer...
January 2015: Biomaterials
https://www.readbyqxmd.com/read/24935525/in-vitro-and-in-vivo-studies-of-bmp-2-loaded-pcl-gelatin-bcp-electrospun-scaffolds
#11
Bo-Ram Kim, Thuy Ba Linh Nguyen, Young-Ki Min, Byong-Taek Lee
To confirm the effect of recombinant human bone morphogenetic protein-2 (BMP-2) for bone regeneration, BMP-2-loaded polycaprolactone (PCL)-gelatin (Gel)-biphasic calcium phosphate (BCP) fibrous scaffolds were fabricated using the electrospinning method. The electrospinning process to incorporate BCP nanoparticles into the PCL-Gel scaffolds yielded an extracellular matrix-like microstructure that was a hybrid system composed of nano- and micro-sized fibers. BMP-2 was homogeneously loaded on the PCL-Gel-BCP scaffolds for enhanced induction of bone growth...
December 2014: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/24785365/infrapatellar-fat-pad-derived-stem-cells-maintain-their-chondrogenic-capacity-in-disease-and-can-be-used-to-engineer-cartilaginous-grafts-of-clinically-relevant-dimensions
#12
Yurong Liu, Conor Timothy Buckley, Henrique V Almeida, Kevin J Mulhall, Daniel John Kelly
A therapy for regenerating large cartilaginous lesions within the articular surface of osteoarthritic joints remains elusive. While tissue engineering strategies such as matrix-assisted autologous chondrocyte implantation can be used in the repair of focal cartilage defects, extending such approaches to the treatment of osteoarthritis will require a number of scientific and technical challenges to be overcome. These include the identification of an abundant source of chondroprogenitor cells that maintain their chondrogenic capacity in disease, as well as the development of novel approaches to engineer scalable cartilaginous grafts that could be used to resurface large areas of damaged joints...
November 2014: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/24559435/smurf1-knocked-down-mesenchymal-stem-cells-and-bmp-2-in-an-electrospun-system-for-bone-regeneration
#13
María Rodríguez-Évora, Emiliano García-Pizarro, Carlos del Rosario, Javier Pérez-López, Ricardo Reyes, Araceli Delgado, José C Rodríguez-Rey, Carmen Évora
A sandwich-like system, fabricated with electrospun, poly(lactic-co-glycolic-acid) (PLGA) membranes incorporating either human recombinant bone morphogenetic protein 2 (BMP-2) enriched microspheres, rat bone marrow mesenchymal stem cells (rMSC), or rMSC with their Smurf1 (SMAD ubiquitin regulatory factor-1) expression knocked down by means of siRNA (rMSC573) at varying densities was evaluated in a rat calvarial, critical-size defect. The behavior of four membrane varieties, fabricated with different PLGA copolymers, was initially studied in rMSC cultures to decide on optimal membrane degradation and cell proliferation and differentiation characteristics...
April 14, 2014: Biomacromolecules
https://www.readbyqxmd.com/read/23956214/osteogenetic-properties-of-electrospun-nanofibrous-pcl-scaffolds-equipped-with-chitosan-based-nanoreservoirs-of-growth-factors
#14
Alice Ferrand, Sandy Eap, Ludovic Richert, Stéphanie Lemoine, Deepak Kalaskar, Sophie Demoustier-Champagne, Hassan Atmani, Yves Mély, Florence Fioretti, Guy Schlatter, Liisa Kuhn, Guy Ladam, Nadia Benkirane-Jessel
Bioactive implants intended for rapid, robust, and durable bone tissue regeneration are presented. The implants are based on nanofibrous 3D-scaffolds of bioresorbable poly-ϵ-caprolactone mimicking the fibrillar architecture of bone matrix. Layer-by-layer nanoimmobilization of the growth factor BMP-2 in association with chitosan (CHI) or poly-L-lysine over the nanofibers is described. The osteogenetic potential of the scaffolds coated with layers of CHI and BMP-2 is demonstrated in vitro, and in vivo in mouse calvaria, through enhanced osteopontin gene expression and calcium phosphate biomineralization...
January 2014: Macromolecular Bioscience
https://www.readbyqxmd.com/read/23515177/the-promotion-of-bone-regeneration-by-nanofibrous-hydroxyapatite-chitosan-scaffolds-by-effects-on-integrin-bmp-smad-signaling-pathway-in-bmscs
#15
Huanhuan Liu, Hongju Peng, Yan Wu, Can Zhang, Youzhi Cai, Guowei Xu, Qin Li, Xiao Chen, Junfeng Ji, Yanzhong Zhang, Hong Wei OuYang
In bone tissue engineering, a combination of biomimetic nanofibrous scaffolds with renewable stem cells has recently emerged as a new strategy for promoting bone regeneration. We have previously developed a biomimetic nanocomposite nanofibrous scaffold of hydroxyapatite/chitosan (nHAp/CTS) [1]. However, the mechanism behind the supportive function of the scaffolds has not yet been adequately explored. Here, we evaluated the effect of nHAp/CTS seeded with bone marrow mesenchymal stem cells (BMSCs) on bone regeneration and examined the underlying mechanism in vitro and in vivo...
June 2013: Biomaterials
https://www.readbyqxmd.com/read/23281203/biological-activity-of-a-nanofibrous-barrier-membrane-containing-bone-morphogenetic-protein-formed-by-core-shell-electrospinning-as-a-sustained-delivery-vehicle
#16
Huiyong Zhu, Dan Yu, Ying Zhou, Chi Wang, Mengfei Gao, Hongliang Jiang, Huiming Wang
This study reports the in vitro and in vivo biological activities of recombinant human bone morphogenetic protein 2 (rhBMP-2) released from the core-shell structure of a nanofibrous barrier membrane as a sustained delivery model for bone regeneration. RhBMP-2 incorporating poly(ethylene glycol) was used as the core, and poly(caprolactone) was used as the shell surrounding the core. To determine its release profiles, the release solution was collected and the amount of rhBMP-2 was measured by ELlSA at different time points...
May 2013: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/23198877/dynamic-regulation-of-bone-morphogenetic-proteins-in-engineered-osteochondral-constructs-by-biomechanical-stimulation
#17
Jin Nam, Priyangi Perera, Bjoern Rath, Sudha Agarwal
Osteochondral tissue-engineered grafts are proposed to hold greater potential to repair/regenerate damaged cartilage through enhanced biochemical and mechanical interactions with underlying subchondral bone as compared to simple engineered cartilage. Additionally, biomechanical stimulation of articular chondrocytes (ACs) or osteoblasts (OBs) was shown to induce greater morphogenesis of the engineered tissues composed of these cells. In this report, to define the advantages of biomechanical stimulation to osteochondral grafts for tissue engineering, we examined whether (1) ACs and OBs in three-dimensional (3D) osteochondral constructs support functional development of each other at the molecular level, and (2) biomechanical stimulation of osteochondral constructs further promotes the regenerative potential of such grafts...
March 2013: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/22718044/functionalisation-of-plla-nanofiber-scaffolds-using-a-possible-cooperative-effect-between-collagen-type-i-and-bmp-2-impact-on-colonization-and-bone-formation-in-vivo
#18
Markus D Schofer, Lisa Tünnermann, Hendric Kaiser, Philip P Roessler, Christina Theisen, Johannes T Heverhagen, Jacqueline Hering, Maximilian Voelker, Seema Agarwal, Turgay Efe, Susanne Fuchs-Winkelmann, Jürgen R J Paletta
The reconstruction of large bone defects after injury or tumor resection often requires the use of bone substitution. Artificial scaffolds based on synthetic biomaterials can overcome disadvantages of autologous bone grafts, like limited availability and donor side morbidity. Among them, scaffolds based on nanofibers offer great advantages. They mimic the extracellular matrix, can be used as a carrier for growth factors and allow the differentiation of human mesenchymal stem cells. Differentiation is triggered by a series of signaling processes, including integrin and bone morphogenetic protein (BMP), which act in a cooperative manner...
September 2012: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/22658161/a-silk-hydrogel-based-delivery-system-of-bone-morphogenetic-protein-for-the-treatment-of-large-bone-defects
#19
Tamim Diab, Eleanor M Pritchard, Brent A Uhrig, Joel D Boerckel, David L Kaplan, Robert E Guldberg
The use of tissue grafting for the repair of large bone defects has numerous limitations including donor site morbidity and the risk of disease transmission. These limitations have prompted research efforts to investigate the effects of combining biomaterial scaffolds with biochemical cues to augment bone repair. The goal of this study was to use a critically-sized rat femoral segmental defect model to investigate the efficacy of a delivery system consisting of an electrospun polycaprolactone (PCL) nanofiber mesh tube with a silk fibroin hydrogel for local recombinant bone morphogenetic protein 2 (BMP-2) delivery...
July 2012: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/22396132/the-impact-of-immobilization-of-bmp-2-on-pdo-membrane-for-bone-regeneration
#20
Ji-Eun Kim, Eun-Jung Lee, Hyoun-Ee Kim, Young-Hag Koh, Jun-Hyeog Jang
Poly(dioxanone) (PDO) is colorless, crystalline, a biodegradable synthetic polymers that is used for biomedical applications, such as surgical sutures, cardiovascular applications, orthopedics, and plastic surgery. Recently, bone morphogenetic protein-2 (BMP-2) is widely used for bone tissue engineering. For the first time we report here on the in vitro performance of an electrospun PDO membrane immobilized with BMP-2. Immobilized BMP-2 on PDO membrane enhanced ALPase activity, the osteogenic differentiation gene expressions as well as cell attachment, except cell proliferation when compared to that of PDO membrane alone...
June 2012: Journal of Biomedical Materials Research. Part A
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