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BMP-2 electrospun

Marion Strub, Xavier Van Bellinghen, Florence Fioretti, Fabien Bornert, Nadia Benkirane-Jessel, Ysia Idoux-Gillet, Sabine Kuchler-Bopp, François Clauss
Current approaches of regenerative therapies constitute strategies for bone tissue reparation and engineering, especially in the context of genetical diseases with skeletal defects. Bone regeneration using electrospun nanofibers' implant has the following objectives: bone neoformation induction with rapid healing, reduced postoperative complications, and improvement of bone tissue quality. In vivo implantation of polycaprolactone (PCL) biomembrane functionalized with BMP-2/Ibuprofen in mouse maxillary defects was followed by bone neoformation kinetics evaluation using microcomputed tomography...
2018: BioMed Research International
Lin Weng, Sunil Kumar Boda, Hongjun Wang, Matthew J Teusink, Franklin D Shuler, Jingwei Xie
An ideal synthetic bone graft is a combination of the porous and nanofibrous structure presented by natural bone tissue as well as osteoinductive biochemical factors such as bone morphogenetic protein 2 (BMP-2). In this work, ultralight 3D hybrid nanofiber aerogels composed of electrospun PLGA-collagen-gelatin and Sr-Cu codoped bioactive glass fibers with incorporation of heptaglutamate E7 domain specific BMP-2 peptides have been developed and evaluated for their potential in cranial bone defect healing. The nanofiber aerogels are surgically implanted into 8 mm × 1 mm (diameter × thickness) critical-sized defects created in rat calvariae...
March 2, 2018: Advanced Healthcare Materials
Ying Qu, BeiYu Wang, BingYang Chu, ChenLu Liu, Xin Rong, Hua Chen, JinRong Peng, ZhiYong Qian
Spinal fusion is the classic treatment to achieve spinal stability for the treatment of the spinal disease. Generally, spinal fusion still has to combine a certain of bone matrix for promoting bone formation to achieve the desired fusion effect based on the surgery, including the traditional bone matrix, such as the autologous bone, allografts and xenografts. Nevertheless, some problems still existed such as the immunogenic problems, the secondary wound, and pathogenic transfer and so on. Here the injectable thermosensitive hydrogel could substitute to avoid the problems as a potential biological scaffold for tissue engineering...
February 7, 2018: ACS Applied Materials & Interfaces
Jingjing Luo, Haitao Zhang, Jiang Zhu, Xiaokang Cui, Junjiu Gao, Xin Wang, Jie Xiong
In the field of bone tissue engineering, an ideal three-dimensional (3-D) scaffold should not only structurally mimic the extracellular matrix (ECM) in large tissues but also mechanically support the bone healing process and provide biochemical cues to induce osteogenesis. In this study, we investigated the feasibility of functionalisation of scaffolds by coupling polyglutamate acid conjugated with BMP-2 peptide onto silk fibroin (SF)/polycaprolactone (PCL) (SF/PCL) blend nanofibers. The morphology, composition, and mineralisation, were confirmed by FE-SEM, XRD, and FT-IR spectroscopy...
March 1, 2018: Colloids and Surfaces. B, Biointerfaces
S Babitha, Meenakshi Annamalai, Michal Marcin Dykas, Surajit Saha, Kingshuk Poddar, Jayarama Reddy Venugopal, Seeram Ramakrishna, Thirumalai Venkatesan, Purna Sai Korrapati
A biomimetic Zein polydopamine based nanofiber scaffold was fabricated to deliver bone morphogenic protein-2 (BMP-2) peptide conjugated titanium dioxide nanoparticles in a sustained manner for investigating its osteogenic differentiation potential. To prolong its retention time at the target site, BMP-2 peptide has been conjugated to titanium dioxide nanoparticles owing to its high surface to volume ratio. The effect of biochemical cues from BMP-2 peptide and nanotopographical stimulation of electrospun Zein polydopamine nanofiber were examined for its enhanced osteogenic expression of human fetal osteoblast cells...
April 2018: Journal of Tissue Engineering and Regenerative Medicine
Vera Lukasova, Matej Buzgo, Vera Sovkova, Jana Dankova, Michala Rampichova, Evzen Amler
OBJECTIVES: Bioactive peptides derived from receptor binding motifs of native proteins are a potent source of bioactive molecules that can induce signalling pathways. These peptides could substitute for osteogenesis promoting supplements. The work presented here compares three kinds of bioactive peptides derived from collagen III, bone morphogenetic protein 7 (BMP-7) and BMP-2 with their potential osteogenic activity on the model of porcine mesenchymal stem cells (pMSCs). MATERIALS AND METHODS: pMSCs were cultured on electrospun polycaprolactone nanofibrous scaffolds with different concentrations of the bioactive peptides without addition of any osteogenic supplement...
August 2017: Cell Proliferation
Raquel Vayas, Ricardo Reyes, María Rodríguez-Évora, Carlos Del Rosario, Araceli Delgado, Carmen Évora
In this study a poly(lactide-co-glycolide) acid (PLGA) tri-layer scaffold is proposed for cartilage repair. The trilayer system consists of a base layer formed by a tablet of PLGA microspheres, a second layer composed of a microsphere suspension placed on top of the tablet, and the third layer, which constitutes an external electrospun PLGA thin polymeric membrane. Combinations of bone morphogenetic protein-2 (BMP-2) encapsulated in the microspheres of the suspension layer, and bone marrow mesenchymal stem cells (bMSC) seeded on the electrospun membrane, are evaluated by histologic analyses and immunohistochemistry in a critical size osteochondral defect in rabbits...
July 4, 2017: Biomedical Materials
Lihua Yin, Shaohua Yang, Miaomiao He, Yuchen Chang, Kaijuan Wang, Yidan Zhu, Yuhui Liu, Yaoren Chang, Zhanhai Yu
Coaxial electrospun fibrous membranes show favorable mechanical properties for use in guided bone regeneration (GBR). We used coaxial electrospinning technology to fabricate three-dimensional nanofiber membranes loaded with BMP-2 and IGF-1, and assessed the physicochemical and biological properties of these novel membranes in vitro. We fabricated four experimental groups of BMP-2/IGF-1/BSA-loaded membranes with different flow ratios (shell/core). Membrane characteristics were assessed by scanning and transmission electron microscopy, and laser confocal microscopy...
June 2017: Journal of Materials Science. Materials in Medicine
Chong Wang, William Weijia Lu, Min Wang
Electrospun scaffolds incorporated with both calcium phosphates (Ca-P) and bone morphogenetic protein-2 (BMP-2) have been used for bone tissue regeneration. However, in most cases BMP-2 and Ca-P were simply mixed and loaded in a monolithic structure, risking low BMP-2 loading level, reduced BMP-2 biological activity, uncontrolled BMP-2 release and inhomogeneous Ca-P distribution. In this investigation, novel bicomponent scaffolds having evenly distributed rhBMP-2-containing fibers and Ca-P nanoparticle-containing fibers were made using an established dual-source dual-power electrospinning technique with the assistance of emulsion electrospinning and blend electrospinning...
August 2017: Journal of Biomedical Materials Research. Part A
Marian H Hettiaratchi, Catherine Chou, Nicholas Servies, Johanna M Smeekens, Albert Cheng, Camden Esancy, Ronghu Wu, Todd C McDevitt, Robert E Guldberg, Laxminarayanan Krishnan
Tissue engineering strategies involving the in vivo delivery of recombinant growth factors are often limited by the inability of biomaterials to spatially control diffusion of the delivered protein within the site of interest. The poor spatiotemporal control provided by porous collagen sponges, which are used for the clinical delivery of bone morphogenetic protein-2 (BMP-2) for bone regeneration, has necessitated the use of supraphysiological protein doses, leading to inflammation and heterotopic ossification...
July 2017: Tissue Engineering. Part A
Lingyan Cao, Yuanman Yu, Jing Wang, Jerome A Werkmeister, Keith M McLean, Changsheng Liu
The aim of this study was to develop a 2-N, 6-O-sulfated chitosan (26SCS) modified electrospun fibrous PCL scaffold for bone morphogenetic protein-2 (BMP-2) delivery to improve osteoinduction. The PCL scaffold was modified by an aminolysis reaction using ethylenediamine (ED) and 26SCS was immobilized via electrostatic interactions (PCL-N-S). Scaffolds were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and contact angle measurements...
May 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Jinkyu Lee, Sajeesh Kumar Madhurakkat Perikamana, Taufiq Ahmad, Min Suk Lee, Hee Seok Yang, Do-Gyoon Kim, Kyobum Kim, Bosun Kwon, Heungsoo Shin
Although bone morphogenetic protein-2 (BMP-2) has been frequently used to stimulate bone formation, it has several side effects to be addressed, including the difficulty in optimization of clinically relevant doses and unwanted induction of cancerous signaling processes. In this study, an osteogenic peptide (OP) derived from BMP-2 was investigated as a substitute for BMP-2. In vitro studies showed that OP was able to enhance the osteogenic differentiation and mineralization of human mesenchymal stem cells (hMSCs)...
April 2017: Tissue Engineering. Part A
Bingjie Niu, Bin Li, Yong Gu, Xiaofeng Shen, Yong Liu, Liang Chen
Bone tissue engineering by using osteoinductive scaffolds seeded with stem cells to promote bone extracellular matrix (ECM) production and remodeling has evolved into a promising approach for bone repair and regeneration. In order to mimic the ECM of bone tissue structurally and compositionally, nanofibrous silk fibroin (SF) scaffolds containing hydroxyapatite (HAP) nanoparticles and bone morphogenetic protein 2 (BMP-2) were fabricated in this study using electrospinning technique. The microstructure, mechanical property, biocompatibility, and osteogenic characteristics were examined...
February 2017: Journal of Biomaterials Science. Polymer Edition
Xia Zhao, David E Komatsu, Michael Hadjiargyrou
The development of effective strategies for gene delivery is a critical goal in DNA-based tissue engineering. Previously, our laboratory utilized the process of electrospinning to fabricate plasmid DNA-based polymeric scaffolds. Although there lease of DNA was robust, the in vitro transfection efficiency was low. In order to optimize these results, we recently modified our approach and utilized a strategy to adsorb plasmid DNA transfection complexes onto a PLLA/Collagen I electrospun scaffold for the delivery of recombinant human Bone Morphogenetic Protein-2 (rhBMP-2)...
June 2016: Journal of Biomedical Nanotechnology
Wei-Han Lin, Jiashing Yu, Guoping Chen, Wei-Bor Tsai
Biofunctional scaffolds that support the adhesion, proliferation, and osteo-differentiation of mesenchymal stem cells (MSCs) are critical for bone tissue engineering. In this study, a simple in situ UV-crosslinking strategy was utilized to fabricate gelatin electrospun fibrous (GEF) scaffolds with multiple biosignals, including cell adhesive Arg-Gly-Asp (RGD) peptide, osteo-conductive hydroxyapatite (HAp) nanoparticles, and osteo-inductive bone morphogenic protein-2 (BMP-2). The adhesion and proliferation of MSCs on the GEF scaffolds were improved by the incorporation of RGD...
February 1, 2016: Colloids and Surfaces. B, Biointerfaces
Tao Yu, Ting Jiang, Qing-mei Wei, Yi-fen Li, David L Kaplan
OBJECTIVE: To identify the healing effect of electrospun silk fibroin-BMP-2 as a biologic pulp capping agent to inflammatory pulp in rat caused by lipopolysaccharide (LPS). METHODS: A total of 30 healthy adult male Wistar rats were randomly divided into five groups:(1) normal control group without operation; (2) blank control group without capping agents; (3) calcium hydroxide capping group; (4) electrospun silk fibroin capping group; (5) electrospun silk fibroin-BMP-2 capping group...
October 18, 2015: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
Shan Ding, Long Li, Xian Liu, Guang Yang, Guangliang Zhou, Shaobing Zhou
In this study, we develop a nano-micro alternating multilayer scaffold for bone tissue engineering by incorporation of monodispersed calcium alginate microbeads into electrospun polymer nanofibers. Both rat bone marrow mesenchymal stem cells (rBMSCs) and bone morphogenetic protein-2 (BMP-2) are simultaneously loaded into the microbeads, which are generated from a microfluidic device. The layer number of the scaffold can be readily controlled by alternately repeating the electrospinning and the microfluidic processes...
September 1, 2015: Colloids and Surfaces. B, Biointerfaces
Sung Yeun Yang, Tae Heon Hwang, Lihua Che, Jin Soo Oh, Yoon Ha, WonHyoung Ryu
Electrospun silk fibroin (SF) scaffolds have drawn much attention because of their resemblance to natural tissue architecture such as extracellular matrix, and the biocompatibility of SF as a candidate material to replace collagen. However, electrospun scaffolds lack the physical integrity of bone tissue scaffolds, which require resistance to mechanical loadings. In this work, we propose membrane-reinforced electrospun SF scaffolds by a serial process of electrospinning and freeze-drying of SF solutions in two different solvents: formic acid and water, respectively...
June 24, 2015: Biomedical Materials
Shijie Chen, Zhiyuan Jian, Linsheng Huang, Wei Xu, Shaohua Liu, Dajiang Song, Zongmiao Wan, Amanda Vaughn, Ruisen Zhan, Chaoyue Zhang, Song Wu, Minghua Hu, Jinsong Li
A mesoporous bioactive glass (MBG) surface modified with poly(lactic-co-glycolic acid) (PLGA) electrospun fibrous scaffold for bone regeneration was prepared by dip-coating a PLGA electrospun fibrous scaffold into MBG precursor solution. Different surface structures and properties were acquired by different coating times. Surface morphology, chemical composition, microstructure, pore size distribution, and hydrophilicity of the PLGA-MBG scaffold were characterized. Results of scanning electron microscopy indicated that MBG surface coating made the scaffold rougher with the increase of MBG content...
2015: International Journal of Nanomedicine
Sajeesh Kumar Madhurakkat Perikamana, Jinkyu Lee, Taufiq Ahmad, Yonghoon Jeong, Do-Gyoon Kim, Kyobum Kim, Heungsoo Shin
Engineering bone tissue is particularly challenging because of the distinctive structural features of bone within a complex biochemical environment. In the present study, we fabricated poly(L-lactic acid) (PLLA) electrospun nanofibers with random and aligned morphology immobilized with bone morphogenic protein-2 (BMP-2) and investigated how these signals modulate (1) in vitro osteogenic differentiation of human mesenchymal stem cells (hMSCs) and (2) in vivo bone growth rate, mechanical properties, and collagen assembly of newly formed bone...
April 29, 2015: ACS Applied Materials & Interfaces
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