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https://www.readbyqxmd.com/read/29341332/cornea-derived-mscs-therapeutically-modulate-macrophage-immunophenotype-and-angiogenic-function
#1
Medi Eslani, Ilham Putra, Xiang Shen, Judy Hamouie, Asha Tadepalli, Khandaker N Anwar, John A Kink, Samaneh Ghassemi, Gaurav Agnihotri, Sofiya Reshetylo, Alireza Mashaghi, Reza Dana, Peiman Hematti, Ali R Djalilian
Macrophages are crucial drivers of inflammatory corneal neovascularization and thus are potential targets for immunomodulatory therapies. We hypothesized that therapeutic use of cornea derived mesenchymal stromal cells (cMSCs) may alter the function of macrophages. We found that cMSCs can modulate the phenotype and angiogenic function of macrophages. In vitro, cMSCs induce apoptosis of macrophages while preferentially promoting a distinct CD14hi CD16hi CD163hi CD206hi immunophenotype that has significantly reduced angiogenic effects based on in vitro angiogenesis assays...
January 17, 2018: Stem Cells
https://www.readbyqxmd.com/read/29332242/selective-ikk2-inhibitor-imd0354-disrupts-nf-%C3%AE%C2%BAb-signaling-to-suppress-corneal-inflammation-and-angiogenesis
#2
Anton Lennikov, Pierfrancesco Mirabelli, Anthony Mukwaya, Mira Schaupper, Muthukumar Thangavelu, Mieszko Lachota, Zaheer Ali, Lasse Jensen, Neil Lagali
Corneal neovascularization is a sight-threatening condition caused by angiogenesis in the normally avascular cornea. Neovascularization of the cornea is often associated with an inflammatory response, thus targeting VEGF-A alone yields only a limited efficacy. The NF-κB signaling pathway plays important roles in inflammation and angiogenesis. Here, we study consequences of the inhibition of NF-κB activation through selective blockade of the IKK complex IκB kinase β (IKK2) using the compound IMD0354, focusing on the effects of inflammation and pathological angiogenesis in the cornea...
January 13, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29325388/-the-research-advances-of-microrna-184-and-related-ocular-diseases
#3
R R Zong, Y P Zhou, Z G Liu
microRNA-184 (miR-184) is a small, non-coding, endogenic RNA molecule of 22 nucleotides in length. It is a highly conserved sequence throughout many different species. Multiple studies have demonstrated that miR-184 is an important factor in regulating gene expression at the post-transcriptional level. miR-184 plays vital roles in many biological processes, including development and differentiation in many tissues and organs. Meanwhile, the research on the physiological and pathological role of miR-184 in eyes draws more and more attention lately...
December 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/29305888/comparative-immunological-study-of-penetrating-and-anterior-lamellar-keratoplasty-in-a-murine-model
#4
Hyun Soo Lee, Ji Young Kwon, Choun-Ki Joo
In lamellar keratoplasty, the diseased part of a cornea is replaced while the healthy tissue remains. lamellar keratoplasty has the advantage of better graft survival compared to penetrating keratoplasty (PK). We compared the immunological responses to PK and anterior lamellar keratoplasty (ALK) in a murine model. PK or ALK was performed using C57BL/6 donor grafts and BALB/c recipients, and graft opacity was assessed to evaluate graft rejection up to 8 weeks. We evaluated the immunological responses in both groups, which were not clinically considered as graft failure on postoperative day 21...
January 3, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29301108/mesenchymal-stromal-cells-inhibit-inflammatory-lymphangiogenesis-in-the-cornea-by-suppressing-macrophage-in-a-tsg-6-dependent-manner
#5
Hyun Beom Song, Se Yeon Park, Jung Hwa Ko, Jong Woo Park, Chang Ho Yoon, Dong Hyun Kim, Jeong Hun Kim, Mee Kum Kim, Ryang Hwa Lee, Darwin J Prockop, Joo Youn Oh
The cornea is a transparent tissue devoid of blood and lymphatic vessels. However, various inflammatory conditions can cause hemangiogenesis and lymphangiogenesis in the cornea, compromising transparency and visual acuity. Mesenchymal stem/stromal cells (MSCs) have therapeutic potentials in a variety of diseases because of anti-inflammatory properties. Herein, we investigated the effects of MSCs on corneal angiogenesis using a model of suture-induced inflammatory corneal neovascularization. Data demonstrated that an intravenous administration of MSCs suppressed corneal inflammation and neovascularization, inhibiting both hemangiogenesis and lymphangiogenesis...
January 3, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29279602/new-therapeutic-perceptions-in-a-patient-with-complicated-herpes-simplex-virus-1-keratitis-a-case-report-and-review-of-the-literature
#6
REVIEW
Dimitrios Kalogeropoulos, Aliki Geka, Konstantinos Malamos, Maria Kanari, Chris Kalogeropoulos
BACKGROUND Keratitis caused by herpes simplex virus (HSV) can have detrimental effects on the cornea leading to loss of vision. Modern therapies can contribute to the prevention of anatomical and functional damage. CASE REPORT An 80-year-old male with complicated HSV-1 keratitis of the left eye (confirmed diagnosis after microbiological investigation) presented three months after antiviral treatment with corneal blurring, severe epitheliopathy, thinning of the stroma, and neovascularization. At the time he was referred, the visual acuity of his left eye was very low, as he could only count fingers at a one-foot distance...
December 27, 2017: American Journal of Case Reports
https://www.readbyqxmd.com/read/29259894/suppression-of-corneal-neovascularization-by-curcumin-via-inhibition-of-wnt-%C3%AE-catenin-pathway-activation
#7
Yong-Kang Zhang, Jing-Ming Li, Li Qin
AIM: To investigate whether curcumin suppressed corneal neovascularization (CNV) formation via inhibiting activation of Wnt/β-catenin pathway. METHODS: Suture-induced CNV was established on Sprague-Dawley (SD) rats. Curcumin were daily administrated by subconjunctival injection. Phosphorylation of low-density lipoprotein receptor-related protein 6 (LRP6) and nuclear accumulation of β-catenin, two indicators of activated Wnt/β-catenin pathway, were determined by Western-blot analysis in subconfluent/proliferating human microvascular endothelial cells (HMEC) and neovascularized corneas...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/29259285/peroxisome-proliferator-activated-receptor-alpha-agonist-suppresses-neovascularization-by-reducing-both-vascular-endothelial-growth-factor-and-angiopoietin-2-in-corneal-alkali-burn
#8
Takeshi Arima, Masaaki Uchiyama, Yuichiro Nakano, Shinya Nagasaka, Dedong Kang, Akira Shimizu, Hiroshi Takahashi
We investigated the effect of a peroxisome proliferator-activated receptor alpha (PPARα) agonist ophthalmic solution in wound healing using a rat corneal alkali burn model. After instillation of a selective agonist of PPARα, fenofibrate, onto the burned cornea, PPARα-positive cells were observed in vascular endothelial cells, and there was upregulation of mRNA of PPARα in corneal stroma. Fenofibrate suppressed expression of neutrophils and macrophages during the early phase, and development of neovascularization and myofibroblast generation during the late phase...
December 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29246498/epithelium-derived-mir-204-inhibits-corneal-neovascularization
#9
Xiaoping Zhang, Guohu Di, Muchen Dong, Mingli Qu, Xiaowen Zhao, Haoyun Duan, Xiaoli Hu, Ting Liu, Qingjun Zhou, Weiyun Shi
MicroRNA-204 (miR-204) is highly expressed in cornea, here we explored the role and mechanism of miR-204 in corneal neovascularization (CNV). Mouse CNV was induced by intrastromal placement of suture in BALB/c mice with the subconjunctival injection of miR-204 agomir or negative control. Human primary limbal epithelial cells (LECs) and immortalized microvascular endothelial cells (HMECs) were used to evaluate the expression changes and anti-angiogenic effects of miR-204 under biomechanical stress (BS). The expression and localization of miR-204, vascular endothelial growth factor (VEGF) and their receptors were detected by quantitative real-time PCR, in situ hybridization, immunohistochemistry and Western blot...
December 12, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/29233043/topical-dimethyl-sulfoxide-inhibits-corneal-neovascularization-and-stimulates-corneal-repair-in-rabbits-following-acid-burn
#10
S Altan, H Sağsöz, Z Oğurtan
Neovascularization of the cornea is characterized by the growth of blood vessels caused by imbalances between angiogenic and anti-angiogenic factors. We investigated whether the expression of Vascular endothelial growth factor (VEGF), Vascular endothelial growth factor receptor (VEGF), Vascular endothelial growth inhibitor (VEGI) receptors, as well as topical drug treatments, participate in regulating corneal neovascularization after corneal damage and remodeling. We used 72 mature male New Zealand rabbits...
December 13, 2017: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29217022/focal-limbal-stem-cell-deficiency-associated-with-soft-contact-lens-wear
#11
Karolien Termote, Steven Schendel, Gregory Moloney, Simon P Holland, Alex P Lange
OBJECTIVE: The aim of this study is to summarize the clinical characteristics of patients with contact lens-associated focal limbal stem cell deficiency (FLSCD) from a tertiary corneal referral centre. DESIGN: Retrospective, observational case series in a tertiary care centre. METHODS: Patients with contact lens-associated FLCSD were identified in our database. Clinical data were retrieved by chart review. A questionnaire asking for contact lens brand, type, cleaning solution, and duration of contact lens wear was sent to the patients with telephone follow-up...
December 2017: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
https://www.readbyqxmd.com/read/29181306/effect-of-itraconazole-on-the-cornea-in-a-murine-suture-model-and-penetrating-keratoplasty-model
#12
Yang Kyung Cho, Eun Young Shin, Hironori Uehara, Balamurali Ambati
AIM: To investigate the anti-(lymph)angiogenic and/or anti-inflammatory effect of itraconazole in a corneal suture model and penetrating keratoplasty (PK) model. METHODS: Graft survival, corneal neovascularization, and corneal lymphangiogenesis were compared among itraconazole, amphotericin B, dexamethasone, phosphate buffered saline (PBS) and surgery-only groups following subconjunctival injection in mice that underwent PK and corneal suture. Immunohistochemical staining and analysis were performed in each group...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/29160014/loss-of-tenascin-x-gene-function-impairs-injury-induced-stromal-angiogenesis-in-mouse-corneas
#13
Takayoshi Sumioka, Hiroki Iwanishi, Yuka Okada, Yuka Nidegawa, Masayasu Miyajima, Ken-Ichi Matsumoto, Shizuya Saika
To determine the contribution by tenascin X (Tnx) gene expression to corneal stromal angiogenesis, the effects were determined of its loss on this response in TNX knockout (KO) mice. In parallel, the effects of such a loss were evaluated on vascular endothelial growth factor (VEGF) and transforming growth factor β1 (TGFβ1) gene and protein expression in fibroblasts and macrophages in cell culture. Histological, immunohistochemical and quantitative RT-PCR changes determined if Tnx gene ablation on angiogenic gene expression, inflammatory cell infiltration and neovascularization induced by central corneal stromal cauterization...
November 21, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29149239/label-free-in-vivo-imaging-of-corneal-lymphatic-vessels-using-microscopic-optical-coherence-tomography
#14
Jens Horstmann, Hinnerk Schulz-Hildebrandt, Felix Bock, Sebastian Siebelmann, Eva Lankenau, Gereon Hüttmann, Philipp Steven, Claus Cursiefen
Purpose: Corneal neovascularization, in particular lymphangiogenesis, is a limiting factor in corneal transplant survival. Novel treatment approaches focus on (selective) inhibition and regression of lymphatic vessels. Imaging clinically invisible corneal lymphatic vessels is a prerequisite for these strategies. Using a murine model, this study investigates whether corneal lymphatic vessels can be imaged using microscopic optical coherence tomography (mOCT). Methods: Corneal neovascularization was induced by intrastromal placement of 11...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29117277/naringenin-eye-drops-inhibit-corneal-neovascularization-by-anti-inflammatory-and-antioxidant-mechanisms
#15
Ana P M T Oguido, Miriam S N Hohmann, Felipe A Pinho-Ribeiro, Jefferson Crespigio, Talita P Domiciano, Waldiceu A Verri, Antonio M B Casella
Purpose: To investigate the effect of naringenin eye drops in corneal neovascularization induced by alkali (1 N NaOH) burn in mice. Methods: Corneal neovascularization in the right eye of male Swiss mice was induced by alkali. Treatment with naringenin eye drops (0.08-80 μg; 8 μL of 0.01-10 g/L solution) or vehicle (saline) started 2 days before corneal neovascularization was induced and was performed twice a day. Mice were treated up until the time animals were euthanized and cornea tissue was collected for testing, which was 2, 4, and 6 hours after alkali stimulus for cytokine and antioxidant capacity measurements, and 3 and/or 7 days after alkali stimulus for the assessment of corneal epithelial thickness and neovascularization, neutrophil, and macrophage recruitment, and vascular endothelial growth factor (Vegf), platelet-derived growth factor (Pdgf), matrix metalloproteinase-14 (Mmp14), and pigment epithelium-derived factor (Pedf) mRNA expression...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29080632/gene-based-antiangiogenic-applications-for-corneal-neovascularization
#16
REVIEW
Siyin Liu, Vito Romano, Bernhard Steger, Stephen B Kaye, Kevin J Hamill, Colin E Willoughby
Corneal avascularity is maintained by angiogenic privilege, an active process involving the production of higher level of angiostatic factors to offset the effect of angiogenic factors. A wide range of pathological insults to the cornea can disrupt this intricate equilibrium and promote angiogenesis and corneal neovascularization with resultant visual impairment. Corneal neovascularization is also a major risk factor for graft failure post-keratoplasty. Current treatment options for corneal neovascularization are restricted by limited efficacy, adverse effects, and a short duration of action...
October 25, 2017: Survey of Ophthalmology
https://www.readbyqxmd.com/read/29075761/corneal-mesenchymal-stromal-cells-are-directly-antiangiogenic-via-pedf-and-sflt-1
#17
Medi Eslani, Ilham Putra, Xiang Shen, Judy Hamouie, Neda Afsharkhamseh, Soroush Besharat, Mark I Rosenblatt, Reza Dana, Peiman Hematti, Ali R Djalilian
Purpose: To evaluate the angiogenic properties of corneal derived mesenchymal stromal cells (Co-MSC). Methods: Co-MSCs were extracted from human cadaver, and wild-type (C57BL/6J) and SERPINF1-/- mice corneas. The MSC secretome was collected in a serum-free medium. Human umbilical vein endothelial cell (HUVEC) tube formation and fibrin gel bead assay (FIBA) sprout formation were used to assess the angiogenic properties of Co-MSC secretome. Complete corneal epithelial debridement was used to induce corneal neovascularization in wild-type mice...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28991852/preparation-and-biomechanical-properties-of-an-acellular-porcine-corneal-stroma
#18
Qing Li, Hongmei Wang, Zhenye Dai, Yichen Cao, Chuanyu Jin
PURPOSE: To construct an acellular porcine corneal stroma (aPCS) as a human corneal stroma alternative and to further explore its biomechanical properties. METHODS: A combination of DNA-RNA enzymes and ultrasound technology was used to strip the native porcine corneal cells. The microstructure of aPCS was observed by H&E staining, DAPI staining, and α-Gal tests. The mechanical properties were detected by a tension machine. Cytotoxicity of aPCS was measured by the MTT assay...
November 2017: Cornea
https://www.readbyqxmd.com/read/28973427/histopathological-and-molecular-changes-in-the-rabbit-cornea-from-arsenical-vesicant-lewisite-exposure
#19
Neera Tewari-Singh, Dinesh G Goswami, Rama Kant, David A Ammar, Dileep Kumar, Robert W Enzenauer, Robert P Casillas, Claire R Croutch, J Mark Petrash, Rajesh Agarwal
Lewisite (LEW), a potent arsenical vesicating chemical warfare agent, poses a continuous risk of accidental exposure in addition to its feared use as a terrorist weapon. Ocular tissue is exquisitely sensitive to LEW and exposure can cause devastating corneal lesions. However, detailed pathogenesis of corneal injury and related mechanisms from LEW exposure that could help identify targeted therapies are not available. Using an established consistent and efficient exposure system, we evaluated the pathophysiology of the corneal injury in New-Zealand white rabbits following LEW vapor exposure (at 0...
September 18, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28954027/effects-of-topical-and-subconjunctival-use-of-bevacizumab-on-corneal-neovascularization-in-rabbits-eyes
#20
Gerson Jorge Aparecido Lopes, Antonio Marcelo Barbante Casella, Ana Paula Oguido, Tiemi Matsuo
Purpose: To evaluate and compare the effects of topical application and subconjunctival injection of bevacizumab on corneal neovascularization (CNV) in rabbits' eyes after chemical burning of the cornea. Methods: The animals were randomly distributed into four groups of five animals. In one group, the drug was instilled, while in another, it was administered by subconjunctival injection. The two procedures using bevacizumab were compared with instillation and subconjunctival injection of saline solution (S)...
July 2017: Arquivos Brasileiros de Oftalmologia
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