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https://www.readbyqxmd.com/read/29160014/loss-of-tenascin-x-gene-function-impairs-injury-induced-stromal-angiogenesis-in-mouse-corneas
#1
Takayoshi Sumioka, Hiroki Iwanishi, Yuka Okada, Yuka Nidegawa, Masayasu Miyajima, Ken-Ichi Matsumoto, Shizuya Saika
To determine the contribution by tenascin X (Tnx) gene expression to corneal stromal angiogenesis, the effects were determined of its loss on this response in TNX knockout (KO) mice. In parallel, the effects of such a loss were evaluated on vascular endothelial growth factor (VEGF) and transforming growth factor β1 (TGFβ1) gene and protein expression in fibroblasts and macrophages in cell culture. Histological, immunohistochemical and quantitative RT-PCR changes determined if Tnx gene ablation on angiogenic gene expression, inflammatory cell infiltration and neovascularization induced by central corneal stromal cauterization...
November 21, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29149239/label-free-in-vivo-imaging-of-corneal-lymphatic-vessels-using-microscopic-optical-coherence-tomography
#2
Jens Horstmann, Hinnerk Schulz-Hildebrandt, Felix Bock, Sebastian Siebelmann, Eva Lankenau, Gereon Hüttmann, Philipp Steven, Claus Cursiefen
Purpose: Corneal neovascularization, in particular lymphangiogenesis, is a limiting factor in corneal transplant survival. Novel treatment approaches focus on (selective) inhibition and regression of lymphatic vessels. Imaging clinically invisible corneal lymphatic vessels is a prerequisite for these strategies. Using a murine model, this study investigates whether corneal lymphatic vessels can be imaged using microscopic optical coherence tomography (mOCT). Methods: Corneal neovascularization was induced by intrastromal placement of 11...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29117277/naringenin-eye-drops-inhibit-corneal-neovascularization-by-anti-inflammatory-and-antioxidant-mechanisms
#3
Ana P M T Oguido, Miriam S N Hohmann, Felipe A Pinho-Ribeiro, Jefferson Crespigio, Talita P Domiciano, Waldiceu A Verri, Antonio M B Casella
Purpose: To investigate the effect of naringenin eye drops in corneal neovascularization induced by alkali (1 N NaOH) burn in mice. Methods: Corneal neovascularization in the right eye of male Swiss mice was induced by alkali. Treatment with naringenin eye drops (0.08-80 μg; 8 μL of 0.01-10 g/L solution) or vehicle (saline) started 2 days before corneal neovascularization was induced and was performed twice a day. Mice were treated up until the time animals were euthanized and cornea tissue was collected for testing, which was 2, 4, and 6 hours after alkali stimulus for cytokine and antioxidant capacity measurements, and 3 and/or 7 days after alkali stimulus for the assessment of corneal epithelial thickness and neovascularization, neutrophil, and macrophage recruitment, and vascular endothelial growth factor (Vegf), platelet-derived growth factor (Pdgf), matrix metalloproteinase-14 (Mmp14), and pigment epithelium-derived factor (Pedf) mRNA expression...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29080632/gene-based-antiangiogenic-applications-for-corneal-neovascularization
#4
REVIEW
Siyin Liu, Vito Romano, Bernhard Steger, Stephen B Kaye, Kevin J Hamill, Colin E Willoughby
Corneal avascularity is maintained by angiogenic privilege, an active process involving the production of higher level of angiostatic factors to offset the effect of angiogenic factors. A wide range of pathological insults to the cornea can disrupt this intricate equilibrium and promote angiogenesis and corneal neovascularization with resultant visual impairment. Corneal neovascularization is also a major risk factor for graft failure post-keratoplasty. Current treatment options for corneal neovascularization are restricted by limited efficacy, adverse effects, and a short duration of action...
October 25, 2017: Survey of Ophthalmology
https://www.readbyqxmd.com/read/29075761/corneal-mesenchymal-stromal-cells-are-directly-antiangiogenic-via-pedf-and-sflt-1
#5
Medi Eslani, Ilham Putra, Xiang Shen, Judy Hamouie, Neda Afsharkhamseh, Soroush Besharat, Mark I Rosenblatt, Reza Dana, Peiman Hematti, Ali R Djalilian
Purpose: To evaluate the angiogenic properties of corneal derived mesenchymal stromal cells (Co-MSC). Methods: Co-MSCs were extracted from human cadaver, and wild-type (C57BL/6J) and SERPINF1-/- mice corneas. The MSC secretome was collected in a serum-free medium. Human umbilical vein endothelial cell (HUVEC) tube formation and fibrin gel bead assay (FIBA) sprout formation were used to assess the angiogenic properties of Co-MSC secretome. Complete corneal epithelial debridement was used to induce corneal neovascularization in wild-type mice...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28991852/preparation-and-biomechanical-properties-of-an-acellular-porcine-corneal-stroma
#6
Qing Li, Hongmei Wang, Zhenye Dai, Yichen Cao, Chuanyu Jin
PURPOSE: To construct an acellular porcine corneal stroma (aPCS) as a human corneal stroma alternative and to further explore its biomechanical properties. METHODS: A combination of DNA-RNA enzymes and ultrasound technology was used to strip the native porcine corneal cells. The microstructure of aPCS was observed by H&E staining, DAPI staining, and α-Gal tests. The mechanical properties were detected by a tension machine. Cytotoxicity of aPCS was measured by the MTT assay...
November 2017: Cornea
https://www.readbyqxmd.com/read/28973427/histopathological-and-molecular-changes-in-the-rabbit-cornea-from-arsenical-vesicant-lewisite-exposure
#7
Neera Tewari-Singh, Dinesh G Goswami, Rama Kant, David A Ammar, Dileep Kumar, Robert W Enzenauer, Robert P Casillas, Claire R Croutch, J Mark Petrash, Rajesh Agarwal
Lewisite (LEW), a potent arsenical vesicating chemical warfare agent, poses a continuous risk of accidental exposure in addition to its feared use as a terrorist weapon. Ocular tissue is exquisitely sensitive to LEW and exposure can cause devastating corneal lesions. However, detailed pathogenesis of corneal injury and related mechanisms from LEW exposure that could help identify targeted therapies are not available. Using an established consistent and efficient exposure system, we evaluated the pathophysiology of the corneal injury in New-Zealand white rabbits following LEW vapor exposure (at 0...
September 18, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28954027/effects-of-topical-and-subconjunctival-use-of-bevacizumab-on-corneal-neovascularization-in-rabbits-eyes
#8
Gerson Jorge Aparecido Lopes, Antonio Marcelo Barbante Casella, Ana Paula Oguido, Tiemi Matsuo
Purpose: To evaluate and compare the effects of topical application and subconjunctival injection of bevacizumab on corneal neovascularization (CNV) in rabbits' eyes after chemical burning of the cornea. Methods: The animals were randomly distributed into four groups of five animals. In one group, the drug was instilled, while in another, it was administered by subconjunctival injection. The two procedures using bevacizumab were compared with instillation and subconjunctival injection of saline solution (S)...
July 2017: Arquivos Brasileiros de Oftalmologia
https://www.readbyqxmd.com/read/28947445/a-study-protocol-for-a-multicentre-randomised-clinical-trial-evaluating-the-safety-and-feasibility-of-a-bioengineered-human-allogeneic-nanostructured-anterior-cornea-in-patients-with-advanced-corneal-trophic-ulcers-refractory-to-conventional-treatment
#9
Miguel González-Andrades, Rosario Mata, María Del Carmen González-Gallardo, Santiago Medialdea, Salvador Arias-Santiago, Juliana Martínez-Atienza, Antonio Ruiz-García, Lorena Pérez-Fajardo, Antonio Lizana-Moreno, Ingrid Garzón, Antonio Campos, Miguel Alaminos, Gloria Carmona, Natividad Cuende
INTRODUCTION: There is a need to find alternatives to the use of human donor corneas in transplants because of the limited availability of donor organs, the incidence of graft complications, as well as the inability to successfully perform corneal transplant in patients presenting limbal deficiency, neo-vascularized or thin corneas, etc. We have designed a clinical trial to test a nanostructured fibrin-agarose corneal substitute combining allogeneic cells that mimics the anterior human native cornea in terms of optical, mechanical and biological behaviour...
September 24, 2017: BMJ Open
https://www.readbyqxmd.com/read/28939878/immunophysical-analysis-of-corneal-neovascularization-mechanistic-insights-and-implications-for-pharmacotherapy
#10
Youness Azimzade, Jiaxu Hong, Alireza Mashaghi
The cornea lacks adaptive immune cells and vasculature under healthy conditions, but is populated by both cell types under pathologic conditions and after transplantation. Here we propose an immunophysical approach to describe postoperative neovascularization in corneal grafts. We develop a simple dynamic model that captures not only the well-established interactions between innate immunity and vascular dynamics but also incorporates the contributions of adaptive immunity to vascular growth. We study how these interactions determine dynamic changes and steady states of the system as well as the clinical outcome, i...
September 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28938378/severe-corneal-disorders-developed-after-brimonidine-tartrate-ophthalmic-solution-use
#11
Yuko Maruyama, Yoko Ikeda, Norihiko Yokoi, Kazuhiko Mori, Hiroaki Kato, Morio Ueno, Shigeru Kinoshita, Chie Sotozono
PURPOSE: The primary side effects associated with 0.1% brimonidine tartrate (BT) ophthalmic solution with sodium chlorite are allergic conjunctivitis, blepharitis, and conjunctival hyperemia. However, cornea-related side effects are rare. In this study, we report 2 similar cases in which corneal neovascularization, corneal infiltration, and corneal opacity developed after BT eye-drop use. METHODS: Retrospective report of 2 cases of corneal infiltration after BT eye-drop use...
December 2017: Cornea
https://www.readbyqxmd.com/read/28932340/contact-lenses-for-keratoconus-current-practice
#12
Marilita M Moschos, Eirini Nitoda, Panagiotis Georgoudis, Miltos Balidis, Eleftherios Karageorgiadis, Nikos Kozeis
BACKGROUND: Keratoconus is a chronic, bilateral, usuallly asymmetrical, non-inflammatory, ectatic disorder, being characterized by progressive steepening, thinning and apical scarring of the cornea. Initially, the patient is asymptomatic, but the visual acuity gradually decreases, resulting in significant vision loss due to the development of irregular astigmatism, myopia, corneal thinning and scarring. The classic treatment of visual rehabilitation in keratoconus is based on spectacles and contact lenses (CLs)...
2017: Open Ophthalmology Journal
https://www.readbyqxmd.com/read/28924439/corneal-neovascularization-with-associated-lipid-keratopathy-in-a-patient-with-obstructive-sleep-apnea-hypopnea-syndrome-using-a-continuous-positive-airway-pressure-machine
#13
Konstantinos Oikonomakis, Myrsini Petrelli, Konstantinos Andreanos, Andreas Mouchtouris, Petros Petrou, Ilias Georgalas, Dimitrios Papaconstantinou, George Kymionis
OBJECTIVE: To report a case of corneal neovascularization with secondary lipid keratopathy in a patient treated with continuous positive airway pressure (CPAP) for obstructive sleep apnea-hypopnea syndrome (OSAHS). CASE REPORT: A 49-year-old male had been diagnosed with obstructive sleep apnea syndrome 10 years ago and has been treated with the application of a CPAP machine during night sleep ever since. For the past year, the patient had been complaining for ocular irritation and excessive tearing of the left eye on awakening...
May 2017: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/28906020/diagnostic-impact-of-anterior-segment-angiography-of-limbal-stem-cell-insufficiency-in-pax6-related-aniridia
#14
Barbara Käsmann-Kellner, Lorenz Latta, Fabian Fries, Arne Viestenz, Berthold Seitz
BACKGROUND: PAX6 is a master gene of ocular development and postnatal ocular equilibrium. Congenital aniridia is the hallmark of PAX6 gene haploinsufficiency (Chr. 11 p. 13), but PAX6-associated aniridia is a profound, progressive pan-ocular developmental disorder often leading to blindness. Limbal stem cell insufficiency and corneal findings in PAX6 syndrome: There is congenital visual impairment with advancing loss of vision mainly due to secondary glaucoma and to corneal blindness caused by limbal stem cell insufficiency (LSCI)...
September 14, 2017: Clinical Anatomy
https://www.readbyqxmd.com/read/28902936/corneal-angiogenesis-based-on-different-protocols-of-alkaline-cauterization-in-murine-models
#15
COMPARATIVE STUDY
Flor Diana Yokoay Claros-Chacaltana, Karina Kamachi Kobashigawa, Ivan Ricardo Martinez Padua, Gisele Pereira Valdetaro, Marcela Aldrovani, José Luiz Laus
Purpose: To establish and compare protocols of alkaline cauterization for inducing corneal angiogenesis in murine models. Methods: Twenty-four adult Wistar rats were distributed into four groups (G1, G2, G3, and G4). The right eye cornea from each rat was cauterized using filter paper (3 mm), soaked in a solution of silver and potassium nitrates (3:1). Cauterization times were 10 (G1 and G4), or 20 seconds (G2 and G3). Cauterized corneas were washed with Ringer's lactate solution...
August 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28889066/effect-of-lentivirus-mediated-gene-silencing-targeting-toll-like-receptor-2-on-corneal-allograft-transplantation-in-rats
#16
Lang Bai, Weiyi Liang, Minting Chen, Yacouba Cissé, Jing Liu, Yaru Su, Jian Yu, Qiong Liu
AIM: The present work aims to assess the effectiveness of lentiviral vector (LV) mediated Toll-like receptor 2 (TLR2) gene silencing in the survival of transplanted corneal allografts, against immune rejection, in rats. METHODS: LV mediated TLR2 small interference RNA (SiRNA) with enhanced green fluorescent protein (eGFP) [LV-TLR2-siRNA-eGFP] was realised and transfected to both rat corneal epithelial (EC) and stromal cells (SC). Multiplicity of infection (MOI) was optimized for transfection efficiency using flow cytometric (FCM) analysis...
November 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28874077/effects-of-sunitinib-and-bevacizumab-on-vegf-and-mirna-levels-on-corneal-neovascularization
#17
Harun Cakmak, Esra Gokmen, Gokay Bozkurt, Tolga Kocaturk, Kemal Ergin
AIM: To evaluate the effects of sunitinib (0.5 mg/ml) and bevacizumab (5 mg/ml) on VEGF-A, VEGFR-2 and microRNA (miRNA) levels on corneal neovascularization (CNV). METHODS: In this study, CNV was induced by silver nitrate application to the cornea, and 40 Albino male rats were equally divided into four subgroups: Group 1 (sunitinib): After silver nitrate application to the cornea, 0.5 mg/ml sunitinib eyedrop was administered twice daily for two weeks (n = 10)...
September 20, 2017: Cutaneous and Ocular Toxicology
https://www.readbyqxmd.com/read/28861341/blockade-of-angiopoietin-2-tie2-signaling-pathway-specifically-promotes-inflammation-induced-angiogenesis-in-mouse-cornea
#18
Zhi-Xin Yan, Yi Luo, Ning-Fei Liu
AIM: To investigate angiopoietin-2 (Ang-2)/Tie2 signaling pathway involving in inflammatory angiogenesis. METHODS: Three interrupted 11-0 nylon sutures were placed into the corneal stroma of BALB/c mice (6wk old) to induce inflammatory neovascularization. Expression of Ang-2 and Tie2 protein on neovascularization were examined by immunofluorescence. The dynamic expression of Ang-2 mRNA on neovascularization was examined by quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR)...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28811496/genome-wide-expression-differences-in-anti-vegf-and-dexamethasone-treatment-of-inflammatory-angiogenesis-in-the-rat-cornea
#19
Pierfrancesco Mirabelli, Anthony Mukwaya, Anton Lennikov, Maria Xeroudaki, Beatrice Peebo, Mira Schaupper, Neil Lagali
Angiogenesis as a pathological process in the eye can lead to blindness. In the cornea, suppression of angiogenesis by anti-VEGF treatment is only partially effective while steroids, although effective in treating inflammation and angiogenesis, have broad activity leading to undesirable side effects. In this study, genome-wide expression was investigated in a suture-induced corneal neovascularization model in rats, to investigate factors differentially targeted by dexamethasone and anti-Vegf. Topical treatment with either rat-specific anti-Vegf, dexamethasone, or normal goat IgG (sham) was given to sutured corneas for 48 hours, after which in vivo imaging, tissue processing for RNA microarray, and immunofluorescence were performed...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28803936/inhibition-of-huvec-tube-formation-via-suppression-of-nf%C3%AE%C2%BAb-suggests-an-anti-angiogenic-role-for-slurp1-in-the-transparent-cornea
#20
Sudha Swamynathan, Chelsea L Loughner, Shivalingappa K Swamynathan
Previously, we have reported that the Secreted Ly6/uPAR related protein-1 (SLURP1) serves an important immunomodulatory function in the ocular surface. Here, we examine the involvement of SLURP1 in regulating corneal angiogenic privilege. Slurp1 expression detected by QPCR, immunoblots and immunofluorescent stain, was significantly decreased in mouse corneas subjected to alkali burn-induced corneal neovascularization (CNV). Addition of exogenous SLURP1 (6XHis-tagged, E. coli expressed and partially purified using Ni-ion columns) significantly suppressed the tumor necrosis factor-α (TNF-α)-stimulated human umbilical cord vascular endothelial cell (HUVEC) tube formation...
November 2017: Experimental Eye Research
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