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specular microscopy

Uri Elbaz, Kamiar Mireskandari, Nasrin Tehrani, Carl Shen, Saad Khan, Sara Luck, Asim Ali
PURPOSE: To establish a normative database of endothelial cell density (ECD) using in-vivo specular microscopy in children under 5 years old. DESIGN: Cross sectional study. METHODS: Specular microscopy was performed during a clinic visit in cooperative children in the standard upright position. In uncooperative children, specular microscopy was performed in the lateral decubitus position under general anesthesia, prior to surgery for other reasons...
October 13, 2016: American Journal of Ophthalmology
Zachary M Mayko, Beth Ann Benetz, Harry Menegay, Christopher P Donovan, Christopher G Stoeger, Mark A Terry, Jonathan H Lass
PURPOSE: To evaluate a single eye bank's measurement of endothelial cell density (ECD) of Descemet membrane endothelial keratoplasty (DMEK) grafts before and after preparation using 2 separate counting methods. METHODS: A series of 60 donor tissues were prepared for DMEK surgery. One to 4 specular images of the central endothelium were taken both before and after preparation, and ECDs were evaluated for a total of 345 unique images. Images were then masked and provided to the Cornea Image Analysis Reading Center (CIARC) for independent analysis...
October 12, 2016: Cornea
Christophe Panthier, Romain Courtin, Emmanuel Guilbert, Damien Gatinel, Alain Saad
PURPOSE: To evaluate the clinical impact of implanting donor tissue torn during preparation for the Descemet membrane endothelial keratoplasty procedure. METHODS: In this single-center retrospective analysis of consecutive cases, 10 cases with torn donor tissue from one surgeon's caseload of 175 procedures were analyzed and compared with control cases. Best-corrected visual acuity, endothelial cell density of the donor tissue measured by specular microscopy, and the recipient's central corneal thickness were recorded preoperatively and again 1, 3, and 6 months after surgery...
October 12, 2016: Cornea
Scott MacRae, Jack T Holladay, Gene Hilmantel, Don Calogero, Samuel Masket, Walter Stark, Adrian Glasser, Eva Rorer, Michelle E Tarver, Tieuvi Nguyen, Malvina Eydelman
No abstract text is available yet for this article.
October 7, 2016: Ophthalmology
Remzi Karadag, Murat Unluzeybek, Ozgur Cakici, Ayse Yagmur Kanra, Huseyin Bayramlar
AIM: The aim of the study is to compare the measurements of central corneal thickness (CCT) performed by two examiners with four different methods at different times inter- and intra-individually. METHODS: Thirty healthy people were included in the study. In these measurements, an optical low-coherence reflectometry (OLCR), an optic coherence tomography (OCT), a specular microscopy (SM), and a corneal topography (CT) were used. Two examiners performed the measurements in a consecutive manner...
October 8, 2016: International Ophthalmology
Mahmood Nejabat, Fatemeh Heidary, Mohammad Reza Talebnejad, Ramin Salouti, Mohammad Hossein Nowroozzadeh, Masoumeh Masoumpour, Hamideh Mahdaviazad, Zahra Tajbakhsh, Maryam Keshtkar, Hossein Jamali, Mohammad Reza Khalili, Hossein Movahedan, Narges Roustaei, Reza Gharebaghi
INTRODUCTION: This study aimed to determine intraocular pressure (IOP) and central corneal thickness (CCT) measurements in healthy Persian children to find clinical reference values in this ethnicity. Additionally, we examined the possible relationship between these measurements. METHODS: This cross-sectional study included 262 eyes of 131 Persian primary school children between 6 and 13 years of age. All eyes were healthy and had no anterior or posterior segment abnormalities, corneal disease, or evidence of glaucoma...
October 5, 2016: Ophthalmology and Therapy
Yuan Zeng, Jian-Hua Gao
OBJECTIVE: This study aimed to evaluate the effect of Mydrin eye-drops on central corneal thickness values and investigate the duration of the effect. METHODS: In this prospective randomised self-controlled study, we enrolled 60 myopic patients (120 eyes) undergoing corneal laser refractive surgery. The central corneal thickness was measured before and one and four hours after administration of Mydrin eye-drops (major components are tropicamide and phenylephrine hydrochloride) using the Orbscan II anterior segment analysis system and a SP-2000P non-contact specular microscope, respectively...
September 29, 2016: Clinical & Experimental Optometry: Journal of the Australian Optometrical Association
Hossein Jamali, Sara Jahanian, Reza Gharebaghi
PURPOSE: To evaluate the effects of prophylactic laser peripheral iridotomy on corneal endothelial cell density and cell morphology in subjects with primary angle closure suspect (PACS) within a one-year follow-up period. METHODS: In this quasi-experimental prospective study, from June 2012 to November 2013, thirty-five PACS eyes underwent laser peripheral iridotomy at clinics affiliated to Shiraz University of Medical Sciences, Shiraz, Iran. After obtaining informed consent, specular microscopy was performed at baseline and at 3-month, 6-month and 12-month follow-up visits...
July 2016: Journal of Ophthalmic & Vision Research
Yumi Hasegawa, Ryohei Nejima, Yosai Mori, Toshihiro Sakisaka, Keiichiro Minami, Kazunori Miyata, Tetsuro Oshika
PURPOSE: The aim of this study was to investigate the factors associated with decreases in corneal endothelial cell density (ECD) resulting from cataract surgery in eyes with pseudoexfoliation syndrome (PEX). METHODS: The clinical records of 78 eyes of 78 patients with PEX who had undergone cataract surgery were reviewed. ECD was measured preoperatively and at 3 months postoperatively with specular microscopy. Multiple regression analysis was used to assess the factors that were significantly related to the rate of ECD loss...
2016: Clinical Ophthalmology
Babu Lal Kumawat, Ranjan Gupta, Arundhati Sharma, Seema Sen, Shikha Gupta, Radhika Tandon
BACKGROUND: Congenital hereditary endothelial dystrophy (CHED) is an autosomal recessive disorder characterized by bilateral, symmetrical, noninflammatory corneal clouding (edema) present at birth or shortly thereafter. This study reports on an unusual delayed presentation of CHED with compound heterozygous SLC4A11 mutations. MATERIALS AND METHODS: A 45-year-old female, presenting with bilateral decreased vision since childhood that deteriorated in the last 5 years, was evaluated to rule out trauma, viral illness, chemical injury, glaucoma, and corneal endothelial dystrophies...
July 2016: Indian Journal of Ophthalmology
Feng Gao, Tao Lin, Yingzhe Pan
Diabetic keratopathy is an ocular complication that occurs with diabetes. In the present study, the effect of diabetic keratopathy on corneal optical density, central corneal thickness, and corneal endothelial cell count was investigated. One hundred and eighty diabetic patients (360 eyes) were enrolled in the study during the period from March, 2012 to March, 2013. The patients were divided into three age groups: <5, 5-10 and >10 years, with 60 patients per group (120 eyes). During the same period, 60 healthy cases (120 eyes) were selected and labeled as the normal control group...
September 2016: Experimental and Therapeutic Medicine
Atsushi Shiraishi, Xiaodong Zheng, Yuri Sakane, Yuko Hara, Yasuhito Hayashi
PURPOSE: To determine the morphological characteristics of the cornea in eyes with posterior corneal vesicles (PCV) determined by in vivo confocal microscopy (IVCM). In addition, to compare the morphological characteristics of the corneas of eyes with PCV to those of eyes with posterior polymorphous corneal dystrophy (PPCD). METHODS: Three males and three females aged 17-75 years who were diagnosed with PCV by slit-lamp biomicroscopic findings were studied. The slit-lamp findings, specular microscopic images, corneal endothelial cell densities (CDs), central corneal thickness, and IVCM images were evaluated...
September 1, 2016: Japanese Journal of Ophthalmology
Hiroshi Takahashi
Phacoemulsification has become the most commonly used procedure in cataract surgery owing to the development of new devices and surgical techniques; however, corneal endothelial damage still represents a serious complication. Although several factors are known to cause corneal endothelial damage, effects of irrigating pressure and temperature rise in the anterior chamber should also be considered. Furthermore, free radical formation is another important harmful factor. Ultrasound in aqueous solution induces cavitation, which results in the formation of hydroxyl free radicals that damage the corneal endothelium...
November 2016: Cornea
Lía Fernández Del Río, Hans Arwin, Kenneth Järrendahl
The optical properties of several scarab beetles have been previously studied but few attempts have been made to compare beetles in the same genus. To determine whether there is any relation between specimens of the same genus, we have studied and classified seven species from the Chrysina genus. The polarization properties were analyzed with Mueller-matrix spectroscopic ellipsometry and the structural characteristics with optical microscopy and scanning electron microscopy. Most of the Chrysina beetles are green colored or have a metallic look (gold or silver)...
July 2016: Physical Review. E
Chris S Ivanoff, Jie Jayne Wu, Hadi Mirzajani, Cheng Cheng, Quan Yuan, Stepan Kevorkyan, Radostina Gaydarova, Desislava Tomlekova
AC electrokinetics (ACEK) has been shown to deliver certain drugs into human teeth more effectively than diffusion. However, using electrical wires to power intraoral ACEK devices poses risks to patients. The study demonstrates a novel interdigitated electrode arrays (IDE) assembly powered by inductive coupling to induce ACEK effects at appropriate frequencies to motivate drugs wirelessly. A signal generator produces the modulating signal, which multiplies with the carrier signal to produce the amplitude modulated (AM) signal...
October 2016: Biomedical Microdevices
Anry Pitchkhadze, Natalia Bilenko, Jaime Levy, Nadav Belfair, Tova Lifshitz
INTRODUCTION: Corneal endothelium is essential for adequate corneal hydration and transparency. Age and ethnicity- related variability in endothelial properties is known. OBJECTIVES: To determine specular microscopy trends in the elderly and to provide normative data of endothelial features of the sampled cohort of the Israeli population aged 55-88 years. METHODS: This is a retrospective cross-sectional study analyzing specular microscopy data of the Israeli population aged 55-88 years and the determination of correlation of age to specular microscopy features...
May 2016: Harefuah
Stefan J Lang, Anja Freysoldt, Daniel Böhringer, Thomas Reinhard, Claudia Auw-Haedrich
PURPOSE: Corneal endothelial cell density and the integrity of the monolayer are essential for maintenance of a clear cornea. In 1992, Williams et al. introduced a method to estimate the endothelial cell density in histopathologic examination. It would enable an evaluation of the corneal host endothelium, even if preoperative measurement was not possible. The goal of this study is to evaluate the accuracy of the Williams equation in corneal buttons obtained from penetrating keratoplasties...
October 2016: Graefe's Archive for Clinical and Experimental Ophthalmology
Argyrios Tzamalis, Chrysanthos Symeonidis, Irini P Brazitikou, Dimitra Tzetzi, Nikos Chalvatzis, Sofia Androudi, Periklis Brazitikos
PURPOSE: To describe the safety and efficacy of removing posteriorly dislocated lens fragments with the use of intravitreal ultrasonic fragmentation through a limbal, clear cornea self-sealing incision. METHODS: Patients presenting with posteriorly luxated nuclei were enrolled in this prospective case series. Preoperative examination included evaluation of the corneal endothelium by means of specular microscopy. A 3-port 25+ pars plana vitrectomy was initially performed...
July 14, 2016: Retina
Thomas S Shute, Ursula M Dietrich, Julia F M Baker, K Paige Carmichael, William Wustenberg, Iqbal Ike K Ahmed, Arsham Sheybani
PURPOSE: The purpose of this study was to evaluate the ocular safety of a novel microfistula implant and its composite materials in an animal model. METHODS: The anterior chambers of 12 rabbit eyes were injected with either glutaraldehyde cross-linked porcine gelatin extract or balanced salt solution and were followed by serial slit lamp examinations over 3 days. The eyes of 18 canines underwent microfistula implantation or a sham procedure. The animals were monitored over the subsequent 12 months, using serial slit lamp examinations, indirect ophthalmoscopy, tonometry, specular microscopy, and high-resolution ultrasonography...
July 1, 2016: Investigative Ophthalmology & Visual Science
Mun Yueh Faria, Nuno Ferreira, Eliana Neto
OBJECTIVE: To report visual outcomes, complication rate, and safety of retropupillary iris-claw intraocular lens (ICIOL) in ectopia lentis in Marfan syndrome (MFS). DESIGN: Retrospective study. METHODS: Six eyes of three MFS patients with ectopia lentis underwent surgery for subluxation lens and retropupillary ICIOL implantation from October 2014 to October 2015 at the Department of Ophthalmology, Santa Maria Hospital in Lisbon, Portugal. Demographics, preoperative and postoperative best-corrected visual acuity (BCVA), and intraocular pressure were evaluated...
2016: International Medical Case Reports Journal
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