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specular microscopy

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https://www.readbyqxmd.com/read/29133638/comparison-of-the-endosaver-with-noninjector-techniques-in-descemet-s-stripping-endothelial-keratoplasty
#1
Michael Tsatsos, Ioannis Athanasiadis, Nikolaos Kopsachilis, Radhika Krishnan, Parwez Hossain, David Anderson
PURPOSE: Minimal loss of corneal endothelial cells during corneal transplantation is a major target but remains a point of controversy among specialists. Hence, the available method to best achieve this continues to stir progress in the field. The aim of this study is to evaluate the use of the Endosaver injector device for graft insertion in Descemet's stripping endothelial keratoplasty (DSEK) and compare the visual outcomes and endothelial cell survival between the Endosaver injector and Goosey grasping forceps insertion techniques...
November 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29120464/insight-on-the-silver-catalyst-distribution-during-silicon-nanowire-array-formation-an-x-ray-reflectivity-study
#2
Jesse W Kremenak, Christopher J Arendse, Franscious R Cummings, Yiyao Chen, Paul F Miceli
Although metal-catalysts are commonly used to create nanoscale materials at surfaces, little is quantitatively known or understood about the depth distribution profile of the catalyst during the growth process. Using X-ray reflectivity, we report the first quantitative investigation, with nanoscale resolution, of the Ag metal-catalyst depth distribution profile during metal-assisted chemical etch (MACE) growth of Si nanowire (SiNW) arrays on Si(100). Given the very low optical reflectivity of these nanowire arrays, specular reflection from these materials in the X-ray region is extremely challenging to measure because it probes interfaces on the nanoscale...
November 9, 2017: Nanoscale
https://www.readbyqxmd.com/read/29119310/on-the-regional-variability-of-averaged-cell-area-estimates-for-the-human-corneal-endothelium-in-relation-to-the-extent-of-polymegethism
#3
M J Doughty
PURPOSE: To assess variability in the coefficient of variation (COV) in cell area estimates when using different numbers of cells for endothelial morphometry. METHODS: Using non-contact specular microscopy images of the corneal endothelium, 4 sets of 20 cases were selected that included 200 cells and had overall (global) COV values of less than 30 (group 1), 31-40 (group 2), 41-50 (group 3) and over 50% (group 4). Subjects could be normal, or had ophthalmic disease (such as diabetes), a history of rigid or soft contact lens wear or were assessed after cataract surgery...
November 7, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/29111821/correlations-of-corneal-optical-densitometry-endothelial-hexagonality-percentage-and-epithelium-thickness
#4
Gökhan Pekel, Fatih Özbakış, Alperen Bahar, Evre Pekel, Ebru Nevin Çetin
PURPOSE: Our aim was to examine the associations between the cornea morphological parameters, endothelial cell density, and anterior chamber measurements. METHODS: This observational study included 50 eyes of 50 healthy volunteers. The thicknesses of corneal epithelium, Bowman's layer, stroma, Descemet's membrane-endothelium complex, and pre-ocular tear film were measured by means of anterior segment optical coherence tomography. The keratometry (K), corneal volume (CV), corneal optical densitometry (COD), iridocorneal angle (ICA), anterior chamber depth (ACD), and anterior chamber volume (ACV) measurements were taken by the Scheimpflug anterior segment imaging...
November 7, 2017: Current Eye Research
https://www.readbyqxmd.com/read/29109893/correlation-of-corneal-endothelial-cell-density-with-corneal-tomographic-parameters-in-eyes-with-keratoconus
#5
Banu Bozkurt, Mevlüt Yılmaz, Ali Meşen, Ümit Kamış, Bengü Ekinci Köktekir, Süleyman Okudan
Objectives: To examine changes in corneal endothelial cell density (ECD) in different stages of keratoconus and evaluate its correlation with corneal tomographic parameters. Materials and Methods: Two hundred six patients with keratoconus were enrolled in the study. Corneal topography was performed by Sirius (CSO, Italy), which has a rotating Scheimpflug camera and a Placido disc topographer. Automatic endothelial analysis was done with the non-contact endothelial microscope (20x probe) of Confoscan-4 (NIDEK, Japan)...
October 2017: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/29090046/preparation-and-thickness-profile-of-endothelial-keratoplasty-lenticules-from-donated-whole-eyes-with-previous-photorefractive-keratectomy
#6
Mozhgan Rezaei Kanavi, Pejman Fahim, Mohsen Rahmanian, Tahereh Chamani, Bahar Kheiri, Sahar Balagholi, Mohammad Ali Javadi
PURPOSE: To describe the preparation and thickness profiles of endothelial keratoplasty lenticules harvested from eyes with previous photorefractive keratectomy (PRK). METHODS: Donor whole eyes that underwent PRK were subjected to microkeratome-assisted dissection for Descemet stripping automated endothelial keratoplasty. Specular microscopy and Visante optical coherence tomography were performed on precut corneas. Endothelial cell indices and thickness profiles of endothelial keratoplasty lenticules were statistically analyzed...
October 2017: Journal of Ophthalmic & Vision Research
https://www.readbyqxmd.com/read/29076809/compact-3d-printed-module-for-fluorescent-and-label-free-imaging-using-evanescent-excitation
#7
Vikas Pandey, Shalini Gupta, Ravikrishnan Elangovan
Total internal reflection fluorescence (TIRF) microscopy is widely used for selective excitation and high-resolution imaging of fluorophores, and more recently label-free nanosized objects, with high vertical confinement near a liquid/solid interface. Traditionally, high numerical aperture (NA) objectives (> 1.4) are used to simultaneously generate evanescent waves and collect fluorescence emission signals which limits their use to small area imaging (< 0.1 mm2). Objective-based TIRFs are also expensive as they require dichroic mirrors and efficient notch filters to prevent specular reflection within the objective lenses...
October 27, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/29073200/torsional-phacoemulsification-a-pilot-study-to-revise-the-harm-scale-evaluating-the-endothelial-damage-and-the-visual-acuity-after-cataract-surgery
#8
Francesco Saverio Sorrentino, Silvia Matteini, Aurelio Imburgia, Claudio Bonifazzi, Adolfo Sebastiani, Francesco Parmeggiani
PURPOSE: To study the effect of torsional phacoemulsification energy on corneal endothelium evaluating the relationship between changes of endothelial cells and postoperative visual acuity. METHODS: This prospective clinical observational cohort study included 50 patients with cataract who underwent torsional phacoemulsification. Sequential quantitative and qualitative morphometric endothelial cell analyses of the cornea were performed four weeks preoperatively and six weeks postoperatively using noncontact specular microscopy...
2017: PloS One
https://www.readbyqxmd.com/read/29059495/2d-spherulites-of-a-semi-fluorinated-alkane-controlled-access-to-either-radial-or-ring-banded-morphologies
#9
Xianhe Liu, Salomé Mielke, Christophe Contal, Damien Favier, Akihisa Yamamoto, Motomu Tanaka, Marie Pierre Krafft
Thin films of a semi-fluorinated alkane cast onto solid substrates consist of well-formed two-dimensional non-birefringent ring-banded and/or radial spherulites. Controlling the experimental conditions allows orientation of the crystallization toward either radial-only or ring-banded-only morphologies. Intermediate states were also captured in which both radial and ring-banded spherulites coexist. Monitoring of the formation of these intermediate states brought evidence for a first crystallization mode that sweeps radially outwards from a central nucleus until the propagating front edge experiences a second crystallization mode that proceeds through a diffusion-controlled rhythmic crystallization mechanism that leads to high (≈2 μm) concentric ridges...
October 23, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/29044058/femtosecond-laser-assisted-cataract-surgery-versus-2-2-mm-clear-corneal-phacoemulsification
#10
H Ranjini, Praveen R Murthy, Gowri J Murthy, Vinay R Murthy
PURPOSE: Phacoemulsification is the surgical procedure of choice for cataract, providing excellent visual and safety outcomes. Femtosecond laser-assisted cataract surgery (FLACS) is an addition to the surgical armamentarium. The study aims to compare the outcomes of FLACS using LenSx™ (Alcon Inc., USA) to standard 2.2 mm clear corneal phacoemulsification. Prospective case-control, comparative, interventional study was conducted in a tertiary care center. METHODS: In each group, 55 eyes of 55 patients underwent cataract surgery using either FLACS or conventional phacoemulsification (control group)...
October 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29041135/improved-performance-of-multi-view-fringe-projection-3d-microscopy
#11
Meng Wang, Yongkai Yin, Dingnan Deng, Xiangfeng Meng, Xiaoli Liu, Xiang Peng
Fringe projection 3D microcopy (FP-3DM) plays an increasingly important role in micro manufacturing and measurement. In recent decades, research on FP-3DM has made considerable progress. Nevertheless, some disadvantages arising from the limited depth of field, local specular reflection and occlusion still exist and need to be further addressed. In this paper, a multi-view FP-3DM (MVFP-3DM) is presented. Four imaging branches with the Scheimpflug condition and one vertical projection branch are deployed to establish the system...
August 7, 2017: Optics Express
https://www.readbyqxmd.com/read/29030797/corneal-endothelial-changes-following-a-single-session-of-selective-laser-trabeculoplasty-for-pseudoexfoliative-glaucoma
#12
Nurgül Örnek, Kemal Örnek
PURPOSE: To evaluate corneal endothelial cell parameters after a single session of selective laser trabeculoplasty (SLT) in pseudoexfoliative glaucoma (PEG) patients. METHODS: Corneal endothelial cell parameters of 18 PEG patients were compared with 18 healthy subjects following SLT treatment. All patients underwent SLT treatment to 180 degrees of inferior trabecular meshwork. Corneal measurements were performed using specular microscopy (Noncon Robo SP8000, Konan Medical, Hyogo, Japan)...
October 13, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/29022296/efficacy-and-safety-of-intracameral-bevacizumab-for-treatment-of-neovascular-glaucoma
#13
Jun Young Ha, Tae Hee Lee, Mi Sun Sung, Sang Woo Park
PURPOSE: To evaluate the long-term efficacy and safety of intracameral bevacizumab in patients with neovascular glaucoma. METHODS: This retrospective study included 26 eyes of 26 neovascular glaucoma patients who received intracameral bevacizumab injection between January 2013 and May 2015, and were followed-up for at least 1 year. All patients were treated with topical and/or systemic intraocular pressure (IOP)-lowering medications, intracameral bevacizumab, and panretinal photocoagulation (PRP)...
August 26, 2017: Korean Journal of Ophthalmology: KJO
https://www.readbyqxmd.com/read/28993974/unique-variations-and-characteristics-of-iridocorneal-endothelial-syndrome-in-china-a-case-series-of-58-patients
#14
Bo Feng, Xin Tang, Hong Chen, Xia Sun, Ningli Wang
PURPOSE: Iridocorneal endothelial (ICE) syndrome is a rare condition, and unique characteristics in Chinese patients can make diagnosis difficult. Our purpose was to describe the clinical characteristics and variations of ICE syndrome in 58 consecutive Chinese patients. METHODS: The clinical data of consecutive patients with ICE syndrome who were seen between 2008 and 2011 at the glaucoma clinic of our ophthalmology department were retrospectively reviewed. The diagnostic criteria for ICE syndrome were a "hammered-silver" appearance of the corneal endothelium and specular microscopy showing ICE cells characterized by the absence of a hexagonal appearance, dark areas within the cells, and a light-dark reversal pattern...
October 10, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28985815/young-donor-graft-assisted-endothelial-keratoplasty-pdek-dmek-with-epithelial-debridement-for-chronic-pseudophakic-bullous-keratopathy
#15
Amar Agarwal, Priya Narang, Dhivya A Kumar, Ashvin Agarwal
OBJECTIVE: The aim of this study was to describe the applicability and report visual outcomes for the treatment of subepithelial fibrosis and anterior stromal scarring in cases of chronic pseudophakic bullous keratopathy (PBK) with epithelial debridement and endothelial keratoplasty (EK) (pre-Descemet endothelial keratoplasty [PDEK]; Descemet membrane endothelial keratoplasty [(DMEK]) using young donor tissue. DESIGN: Prospective, single-centre, interventional study...
October 2017: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
https://www.readbyqxmd.com/read/28979090/effects-of-temperature-and-fluid-media-on-the-scroll-width-size-of-the-descemet-s-membrane-endothelial-keratoplasty-dmek-donor-graft
#16
Ellen H Koo, Allen O Eghrari, Ryan S Meshkin, Wei Shi, William J Feuer, Karen G DeMarco, Arthur C Kurz
AIM: Our study was conducted to evaluate whether higher temperature leads to increased - or wider - scroll widths of the Descemet's membrane endothelial keratoplasty (DMEK) donor graft. PURPOSE: To investigate the effects of temperature and fluid media on the DMEK donor graft scroll widths. MATERIALS AND METHODS: This research work was a laboratory investigation. Baseline cell count was taken via specular microscopy for the donor corneas at room temperature (20°C-25°C)...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/28972777/coupled-effects-of-spreading-solvent-molecules-and-electrostatic-repulsions-on-the-behavior-of-ps-b-paa-monolayers-at-the-air-water-interface
#17
Zineb Guennouni, Michel Goldmann, Marie-Claude Fauré, Philippe Fontaine, Patrick Perrin, Denis Limagne, Fabrice Cousin
We describe the surface behavior of PS-b-PAA monolayers at the air/water interface using N,N-dimethyformamide (DMF) as spreading solvent. At low pH, when the PAA blocks are neutral, the surface pressure versus molecular area isotherm shows a pseudoplateau associated with the presence of remaining spreading solvent molecules in the monolayer, as we described in a former study (Guennouni et al., Langmuir, 2016). We show here that the width of the plateau decreases when increasing pH up to its complete disappearance at high pH, when PAA blocks are fully charged, although two regimes of compressibilities on the isotherm still exist...
October 23, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28938379/dark-endothelial-spots-after-descemet-membrane-endothelial-keratoplasty-may-appear-as-recurrent-fuchs-dystrophy-or-herald-graft-failure-or-rejection
#18
Vasiliki Zygoura, Lamis Baydoun, Claire Monnereau, Maria Satué, Silke Oellerich, Gerrit R J Melles
PURPOSE: To evaluate the clinical significance of dark spots in the donor endothelial cell layer as observed with specular microscopy, in patients who underwent Descemet membrane endothelial keratoplasty (DMEK) for Fuchs endothelial dystrophy (FED). METHODS: Specular microscopy images of 83 consecutive eyes up to 7 years after DMEK were retrospectively reviewed in a masked fashion for the presence of dark spots and morphologic changes in the endothelial cell layer and processed for endothelial cell density (ECD) measurements...
December 2017: Cornea
https://www.readbyqxmd.com/read/28914231/plasmonic-resonances-in-hybrid-systems-of-aluminum-nanostructured-arrays-and-few-layer-graphene-within-the-uv-ir-spectral-range
#19
Ricardo González-Campuzano, J M Saniger, Doroteo Mendoza
The size-controllable and ordered Al nanocavities and nanodomes arrays were synthesized by electrochemical anodization of aluminum using phosphoric acid, citric acid and mixture both acids. Few layer graphene (FLG) was transferred directly on top of Al nanostructures and their morphology were evaluated by Scanning Electron Microscopy. The interaction between FLG and the plasmonic properties of Al nanostructures arrays were investigated based on specular reflectivity in the UV-Vis-IR range and Raman Spectroscopy...
September 15, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28886202/fuchs-endothelial-corneal-dystrophy-and-rna-foci-in-patients-with-myotonic-dystrophy
#20
V Vinod Mootha, Brock Hansen, Ziye Rong, Pradeep P Mammen, Zhengyang Zhou, Chao Xing, Xin Gong
Purpose: The most common cause of Fuchs' endothelial corneal dystrophy (FECD) is an intronic CTG repeat expansion in TCF4. Expanded CUG repeat RNA colocalize with splicing factor, muscleblind-like 1 (MBNL1), in nuclear foci in endothelium as a molecular hallmark. Myotonic dystrophy type 1 (DM1) is a neuromuscular disorder caused by a CTG repeat expansion in the 3'-untranslated region (UTR) of DMPK. In this study, we examine for RNA-MBNL1 foci in endothelial cells of FECD subjects with DM1, test the hypothesis that DM1 patients are at risk for FECD, and determine prevalence of TCF4 and DMPK expansions in a FECD cohort...
September 1, 2017: Investigative Ophthalmology & Visual Science
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