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specular microscopy

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https://www.readbyqxmd.com/read/28060145/corneal-endothelial-cell-density-and-morphology-in-different-trimesters-of-pregnancy
#1
Nurgül Örnek, Zeynep Özcan Dağ, Kemal Örnek
OBJECTIVES: To evaluate corneal endothelial cell density (CD) and morphology in different trimesters of pregnancy. METHODS: This prospective study included 66 (66 eyes) pregnant women. The patient group was divided into three subgroups comprised of 22 women in the first, second, and third trimesters of pregnancy. An age-matched group of 22 (22 eyes) volunteers served as controls. Corneal measurements were performed using specular microscopy (Noncon Robo SP8000, Konan Medical)...
January 4, 2017: Eye & Contact Lens
https://www.readbyqxmd.com/read/28060074/eventual-endothelial-failure-after-initial-corneal-clearing-after-a-detached-endothelial-graft-in-fuchs-dystrophy
#2
Hyunjoo J Lee, Wuqaas M Munir
PURPOSE: To report a case of eventual corneal endothelial cell failure after spontaneous resolution of corneal edema after failed Descemet membrane endothelial keratoplasty (DMEK). METHODS: Retrospective case report. RESULTS: A 56-year-old man with Fuchs endothelial corneal dystrophy underwent cataract and DMEK surgery in the left eye. The transplanted DMEK graft detached in the early postoperative period and formed a peripheral scroll. Despite graft detachment, corneal edema resolved with endothelial cell repopulation of the central cornea...
February 2017: Cornea
https://www.readbyqxmd.com/read/28060070/enhancing-dmek-success-by-identifying-optimal-levels-of-trypan-blue-dye-application-to-donor-corneal-tissue
#3
Parag A Majmudar, Lauren Johnson
PURPOSE: The purpose of this study was to determine the correct concentration and duration of application of trypan blue dye to Descemet membrane endothelial keratoplasty (DMEK) donor corneal tissue before transplant to enhance visibility while minimizing damage to the tissue. METHODS: Corneas were precut for DMEK using an 8-mm punch, and each was stained with either 0.06% or 0.15% trypan blue for a duration of 1, 3, or 5 minutes. After staining, each tissue was placed in balanced salt solution and observed to determine how quickly the stain faded from the tissue using a grading system...
February 2017: Cornea
https://www.readbyqxmd.com/read/28060061/conventional-and-iontophoresis-corneal-cross-linking-for-keratoconus-efficacy-and-assessment-by-optical-coherence-tomography-and-confocal-microscopy
#4
Léa Jouve, Vincent Borderie, Otman Sandali, Cyrille Temstet, Elena Basli, Laurent Laroche, Nacim Bouheraoua
PURPOSE: To compare the efficacy, safety, and microstructural corneal changes during 2 years after conventional corneal collagen cross-linking (C-CXL) and transepithelial corneal CXL by iontophoresis (I-CXL) for keratoconus. METHODS: Eighty eyes of 80 patients with progressive keratoconus were treated by C-CXL (n = 40) or I-CXL (n = 40). Patients were investigated before surgery and 1, 3, 6, 12, and 24 months after treatment. We measured central corneal thickness and maximal simulated keratometry values (Kmax) and performed specular microscopy and in vivo confocal microscopy at each time point...
February 2017: Cornea
https://www.readbyqxmd.com/read/28009792/clinical-features-in-children-with-posterior-polymorphous-corneal-dystrophy
#5
Ye Jin Ahn, Soon Il Choi, Hae Ri Yum, Sun Young Shin, Shin Hae Park
PURPOSE: To describe clinical features in children diagnosed with posterior polymorphous corneal dystrophy (PPCD) in their first or second decade of life. METHODS: A retrospective study was performed with the medical records of seven unrelated Korean pediatric patients who were diagnosed with PPCD and were followed up for a minimum of 3 years. Thorough ocular examinations were performed, including best-corrected visual acuity, intraocular pressure, refractive and keratometric measurements, slit-lamp biomicroscopy, and specular microscopy at all visits...
December 22, 2016: Optometry and Vision Science: Official Publication of the American Academy of Optometry
https://www.readbyqxmd.com/read/28003782/corneal-endothelial-cell-density-in-healthy-caucasian-population
#6
Rahmi Duman, Mediha Tok Çevik, Sadık Görkem Çevik, Reşat Duman, İrfan Perente
AIM: To reveal the changes of corneal endothelial characteristics with aging among Caucasian population. METHODS: Non-contact specular microscopy was performed in 564 eyes of 282 healthy Caucasian Turkish patients. Endothelial cell density (MCD), mean cell area (MCA), coefficient of variation (CV) in cell size, percentage of hexagonal cells, and central corneal thickness (CCT) were measured. RESULTS: The mean age was 42 ± 17.1 (6-85) years...
October 2016: Saudi Journal of Ophthalmology: Official Journal of the Saudi Ophthalmological Society
https://www.readbyqxmd.com/read/27998103/nb2o5-nanostructure-evolution-on-nb-surfaces-via-low-energy-he-ion-irradiation
#7
Theodore Joseph Novakowski, Jitendra Kumar Tripathi, Ahmed Hassanein
We propose low-energy, broad-beam He(+) ion irradiation as a novel processing technique for the generation of Nb2O5 surface nanostructures due to its relative simplicity and scalability in a commercial setting. Since there have been relatively few studies involving the interaction of high-fluence, low-energy He(+) ion irradiation and Nb (or its oxidized states), this systematic study explores both effects of fluence and sample temperature during irradiation on resulting surface morphology. Detailed normal and cross-sectional scanning electron microscopy (SEM) studies reveal subsurface He bubble formation and elucidate potential driving mechanisms for nanostructure evolution...
December 21, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27984505/relationship-between-corneal-thickness-and-radius-to-body-height
#8
Sven Jonuscheit, Michael J Doughty, Raúl Martín, Ana Rio-Cristobal
PURPOSE: To investigate the possible association between body stature (height) and corneal thickness and radius in younger-adult Caucasians, especially within the context of previously published literature. METHODS: Body height and weight were measured in 109 healthy subjects, with an average age of 24 ± 6 years (mean ± SD). Subjects underwent an ophthalmic assessment including anterior segment imaging by Scheimpflug topography and specular microscopy. Central and peripheral corneal thickness and corneal radius were analyzed...
December 15, 2016: Optometry and Vision Science: Official Publication of the American Academy of Optometry
https://www.readbyqxmd.com/read/27982210/comparison-of-central-corneal-thickness-estimated-by-an-ultrasonic-pachymeter-and-non-contact-specular-microscopy
#9
Sadık Görkem Çevik, Rahmi Duman, Mediha Tok Çevik, Sertaç Argun Kıvanç, Berna Akova-Budak, Irfan Perente, Resat Duman
Purpose: To compare central corneal thickness (CCT) measurements of healthy individuals obtained with ultrasonic pachymetry (UP) and non-contact specular microscopy (NCSM). Method: In total, 148 eyes of 74 subjects with no ocular or systemic diseases were included in the study. Central corneal thickness measurements of all patients performed with UP and NCCM were compared. Results: A total of 74 subjects (38 females) were included in this study...
September 2016: Arquivos Brasileiros de Oftalmologia
https://www.readbyqxmd.com/read/27980360/changes-in-corneal-endothelial-cell-after-ahmed-glaucoma-valve-implantation-and-trabeculectomy-1-year-follow-up
#10
Min Su Kim, Kyoung Nam Kim, Chang-Sik Kim
PURPOSE: To compare changes in corneal endothelial cell density (CECD) after Ahmed glaucoma valve (AGV) implantation and trabeculectomy. METHODS: Changes in corneal endothelium in patients that underwent AGV implantation or trabeculectomy were prospectively evaluated. Corneal specular microscopy was performed at the central cornea using a non-contact specular microscope before surgery and 6 months and 12 months after surgery. The CECD, hexagonality of the endothelial cells, and the coefficient of variation of the cell areas were compared between the two groups...
December 2016: Korean Journal of Ophthalmology: KJO
https://www.readbyqxmd.com/read/27977477/iridocorneal-endothelial-syndrome-presenting-with-large-diurnal-intraocular-pressure-fluctuation
#11
Rachel S Mogil, Jung Min Lee, Andrew Tirsi, Celso Tello, Sung Chul Park
PURPOSE OF THE STUDY: The purpose of the study was to report a case of iridocorneal endothelial syndrome with an initial presentation of a large diurnal fluctuation of intraocular pressure (IOP) which peaked early in the morning. METHODS: A 31-year-old white man had transient blurry vision oculus sinister (OS) upon awakening in the morning for the past 2 months. The blurry vision improved within 1 to 2 hours. Clinical examination, diurnal IOP measurements, and specular microscopy were performed to investigate the cause of his transient blurry vision...
December 13, 2016: Journal of Glaucoma
https://www.readbyqxmd.com/read/27965261/very-early-endothelial-cell-loss-after-penetrating-keratoplasty-with-organ-cultured-corneas
#12
Anne Sophie Gauthier, Thibaud Garcin, Gilles Thuret, Zhiguo He, Remy Jullienne, Marie Caroline Trone, Chaker Nefzaoui, Sophie Acquart, Fabien Forest, Michel Péoc'h, Bernard Delbosc, Philippe Gain
AIMS: After keratoplasty, postoperative endothelial cell loss is calculated between the eye bank endothelial cell density (ebECD) and the postoperative specular microscopy (SM). To elucidate the very early cell loss, always described after penetrating keratoplasty (PK), we designed two complementary studies. METHODS: (1) Clinical prospective study of 90 consecutive PKs (keratoconus, Fuchs' corneal dystrophy, lattice dystrophy, bullous keratopathy) with organ-cultured corneas and postoperative follow-up by SM at day 5 (D5), D15, month 1 (M1) and M3...
December 13, 2016: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/27899387/activation-of-cytomegalovirus-corneal-endotheliitis-following-laser-in-situ-keratomileusis
#13
Tien-En Tan, Chui Ming Gemmy Cheung, Jodhbir S Mehta
A case of Cytomegalovirus (CMV) corneal endotheliitis following laser in-situ keratomileusis (LASIK) is presented. A 32-year-old man presented 3 weeks after uncomplicated myopic LASIK with unilateral LASIK flap oedema, interface fluid accumulation, keratic precipitates, anterior uveitis and raised intraocular pressure. Despite treatment with topical corticosteroids, he had 3 further recurrent episodes. Specular microscopy showed decreased endothelial cell density and aqueous humour. Polymerase chain reaction (PCR) testing was positive for CMV DNA...
November 29, 2016: BMJ Case Reports
https://www.readbyqxmd.com/read/27878429/comparison-of-specular-microscopy-and-ultrasound-pachymetry-before-and-after-cataract-surgery
#14
Alberto López-Miguel, María Sanchidrián, Itziar Fernández, Alfredo Holgueras, Miguel J Maldonado
PURPOSE: To compare specular microscopy (Topcon SP-3000P, Topcon Corporation, Tokyo, Japan) and ultrasound (US) technology when evaluating central corneal thickness (CCT) prior to and after phacoemulsification. METHODS: Corneal edema was assessed in phacoemulsification patients due to senile cataract by measuring CCT preoperatively and 1 day, 1 month, and 3 months postoperatively. Bland-Altman analysis was performed to assess interchangeability between pachymetry techniques for each visit...
November 22, 2016: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/27861306/enhancing-dmek-success-by-identifying-optimal-levels-of-trypan-blue-dye-application-to-donor-corneal-tissue
#15
Parag A Majmudar, Lauren Johnson
PURPOSE: The purpose of this study was to determine the correct concentration and duration of application of trypan blue dye to Descemet membrane endothelial keratoplasty (DMEK) donor corneal tissue before transplant to enhance visibility while minimizing damage to the tissue. METHODS: Corneas were precut for DMEK using an 8-mm punch, and each was stained with either 0.06% or 0.15% trypan blue for a duration of 1, 3, or 5 minutes. After staining, each tissue was placed in balanced salt solution and observed to determine how quickly the stain faded from the tissue using a grading system...
November 16, 2016: Cornea
https://www.readbyqxmd.com/read/27856284/multiwavelength-micromirrors-in-the-cuticle-of-scarab-beetle-chrysina-gloriosa
#16
Gonzague Agez, Chloé Bayon, Michel Mitov
: Beetles from the genus Chrysina show vivid reflections from bright green to metallic silver-gold as a consequence of the cholesteric liquid crystal organization of chitin molecules. Particularly, the cuticle of Chrysina gloriosa exhibits green and silver stripes. By combining confocal microscopy and spectrophotometry, scanning electron microscopy and numerical simulations, the relationship between the reflectance and the structural parameters for both stripes at the micro- and nanoscales are established...
November 14, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27827347/imaging-pulsed-laser-deposition-growth-of-homo-epitaxial-srtio3-by-low-energy-electron-microscopy
#17
A J H van der Torren, S J van der Molen, J Aarts
By combining low-energy electron microscopy with in situ pulsed laser deposition we have developed a new technique for film growth analysis, making use of both diffraction and real-space information. Working at the growth temperature, we can use: the intensity and profile variations of the specular beam to follow the coverage in a layer-by-layer fashion; real-space microscopy to follow e.g. atomic steps at the surface; and electron reflectivity to probe the unoccupied band structure of the grown material. Here, we demonstrate our methodology for homo-epitaxial growth of SrTiO3...
November 9, 2016: Nanotechnology
https://www.readbyqxmd.com/read/27824383/360-degree-iris-burns-following-conductive-keratoplasty
#18
Hanefi Çakir, Selim Genç, Emre Güler
PURPOSE: The authors report a case with multiple iris burns after conductive keratoplasty to correct hyperopia. METHODS: Case report. RESULTS: A 52-year-old woman with hyperopia had a previous conductive keratoplasty procedure and underwent a conductive keratoplasty re-treatment 6 months later. Postoperatively, she presented with 360-degree iris burns in both eyes that were correlated with the corneal conductive keratoplasty scars. In addition, specular microscopy revealed decreased endothelial cell density for both eyes...
November 1, 2016: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/27746297/corneal-endothelial-cell-density-in-children-normative-data-from-birth-to-5-years-old
#19
Uri Elbaz, Kamiar Mireskandari, Nasrin Tehrani, Carl Shen, Muhammad Saad Khan, Sara Williams, Asim Ali
PURPOSE: To establish a normative database of endothelial cell density (ECD) using in vivo specular microscopy in children under 5 years old. DESIGN: Cross-sectional study. METHODS: Specular microscopy was performed during a clinic visit in cooperative children in the standard upright position. In uncooperative children, specular microscopy was performed in the lateral decubitus position under general anesthesia, before surgery for other reasons...
January 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/27741015/donor-endothelial-cell-density-measurements-do-not-change-immediately-after-dmek-preparation
#20
Zachary M Mayko, Beth Ann Benetz, Harry Menegay, Christopher P Donovan, Christopher G Stoeger, Mark A Terry, Jonathan H Lass
PURPOSE: To evaluate a single eye bank's measurement of endothelial cell density (ECD) of Descemet membrane endothelial keratoplasty (DMEK) grafts before and after preparation using 2 separate counting methods. METHODS: A series of 60 donor tissues were prepared for DMEK surgery. One to 4 specular images of the central endothelium were taken both before and after preparation, and ECDs were evaluated for a total of 345 unique images. Images were then masked and provided to the Cornea Image Analysis Reading Center (CIARC) for independent analysis...
October 12, 2016: Cornea
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