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https://www.readbyqxmd.com/read/28066145/effects-of-high-glucose-with-or-without-other-metabolic-substrates-on-alpha-adrenergic-contractions-in-rat-mesenteric-and-femoral-arteries
#1
Rany Vorn, Hae Young Yoo
Hyperglycemia is associated with an increased risk of cardiovascular diseases. It has been demonstrated that chronic exposure to high glucose impaired endothelial functions. However, specific effects of short-term exposure to high glucose on vascular reactivity are controversial. Moreover, the combined effects of other metabolic substrates such as free fatty acids (FFA) on vascular reactivity remain poorly understood. Here we investigate the effects of short-term exposure to high glucose with or without other metabolic substrates including FFAs termed "nutrition full" (NF) solution, on mesenteric (MA) and deep femoral arteries (DFA) of rats...
January 2017: Korean Journal of Physiology & Pharmacology
https://www.readbyqxmd.com/read/28060318/isolation-and-primary-culture-of-mouse-aortic-endothelial-cells
#2
Jie-Mei Wang, Alex F Chen, Kezhong Zhang
The vascular endothelium is essential to normal vascular homeostasis. Its dysfunction participates in various cardiovascular disorders. The mouse is an important model for cardiovascular disease research. This study demonstrates a simple method to isolate and culture endothelial cells from the mouse aorta without any special equipment. To isolate endothelial cells, the thoracic aorta is quickly removed from the mouse body, and the attached adipose tissue and connective tissue are removed from the aorta. The aorta is cut into 1 mm rings...
December 19, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28060075/removal-and-repositioning-of-intracorneal-ring-segments-improving-corneal-topography-and-clinical-outcomes-in-keratoconus-and-ectasia
#3
Kahei Chan, Peter S Hersh
PURPOSE: To evaluate the efficacy of removal and relocation of intracorneal ring segments for improving outcomes in treatment of keratoconus and corneal ectasia. METHODS: This is a retrospective case series conducted at a cornea and refractive surgery subspecialty practice setting. Patients with previous insertion of 2 intracorneal ring segments underwent surgical removal and repositioning of segments because of unsatisfactory visual and topographic outcomes. The principal outcomes included uncorrected and corrected visual acuities, manifest refraction, topography-derived maximum keratometry (Kmax), inferior-superior topography power difference (I - S), and higher-order aberration profile derived from wavefront analysis...
February 2017: Cornea
https://www.readbyqxmd.com/read/28060059/efficacy-of-intrastromal-corneal-ring-segments-combined-with-flash-collagen-cross-linking-in-keratoconus
#4
Carlos H Gordillo, Juan C Grandin, Roger Zaldivar, Roberto Zaldivar, Adriana C Lotfi
PURPOSE: To evaluate outcomes, efficacy, and safety of intrastromal corneal ring segment implantation for treatment of keratoconus combined with collagen cross-linking (CXL) with 1-year follow-up. METHODS: One hundred nineteen eyes of 82 patients with keratoconus (37 females) underwent femtosecond (Intralase FS; Abbott Medical Optics, Inc) laser-assisted intrastromal corneal ring segment implantation (INTRASEG; Gamma Vision) with flash CXL (18 mW/cm for 5 minutes)...
February 2017: Cornea
https://www.readbyqxmd.com/read/28060027/advantages-of-anterior-segment-optical-coherence-tomography-evaluation-of-the-kayser-fleischer-ring-in-wilson-disease
#5
Mittanamalli S Sridhar
PURPOSE: To present anterior segment optical coherence tomography (AS-OCT) findings of the Kayser-Fleischer (KF) ring in Wilson disease (WD) and to discuss the potential advantages of evaluating the KF ring by AS-OCT. METHODS: This is a retrospective case series of 7 patients with WD with KF rings seen in our institute during the study period (August 2015 to June 2016). All patients underwent slit-lamp examination of the cornea and AS-OCT (Optovue RTVue Premier)...
January 4, 2017: Cornea
https://www.readbyqxmd.com/read/28056120/clinical-characterization-of-cngb1-related-autosomal-recessive-retinitis-pigmentosa
#6
Sarah Hull, Marcella Attanasio, Gavin Arno, Keren Carss, Anthony G Robson, Dorothy A Thompson, Vincent Plagnol, Michel Michaelides, Graham E Holder, Robert H Henderson, F Lucy Raymond, Anthony T Moore, Andrew R Webster
Importance: There are limited published data on the phenotype of retinitis pigmentosa (RP) related to CNGB1 variants. These data are needed both for prognostic counseling of patients and for understanding potential treatment windows. Objective: To describe the detailed clinical and molecular genetic findings in a series of patients with RP with likely pathogenic variants in CNGB1. Design, Setting, and Participants: In this case series, 10 patients from 9 families underwent full ophthalmologic examination...
January 5, 2017: JAMA Ophthalmology
https://www.readbyqxmd.com/read/28047142/we-ab-bra-04-investigation-of-mri-derived-thermal-dose-models
#7
C MacLellan, D Fuentes, H Espinoza, S Prabhu, G Rao, J Weinberg, R Stafford
PURPOSE: To develop and investigate a novel thermal dose model methodology using MR temperature imaging (MRTI) and post-treatment MRI changes as a surrogate of tissue damage in clinical laser ablation of the brain. METHODS: Post-treatment contrast enhanced T1-weighted images were registered to MRTI acquired during two MR-guided thermal ablation procedures. The non-enhancing central region and hyperintense ring of vascular disruption characteristic of the thermal lesion were segmented manually and resampled into the 2D geometry of the MRTI...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28035448/lipoinjection-and-multiple-internal-cuts-for-congenital-constriction-bands-a-new-treatment-approach
#8
Yanko Castro-Govea, Amin Vela-Martinez, Luis Alberto Treviño-Garcia
BACKGROUND: Traditional treatment for a congenital constriction band of the limb involves multiple Z-plasties and W-plasties. We propose an alternative surgical procedure for the treatment of congenital constriction bands that obviates the need for Z-plasties and eliminates the constriction band. METHODS: We present the case of a 36-year-old woman with a congenital constriction band of the leg. Using a minimally invasive approach, the skin segment that included the band was dissected from the deep tissues...
December 29, 2016: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28024654/coronary-computed-tomography-angiography-derived-plaque-quantification-in-patients-with-acute-coronary%C3%A2-syndrome
#9
Christian Tesche, Damiano Caruso, Carlo N De Cecco, Darby C Shuler, Jess D Rames, Moritz H Albrecht, Taylor M Duguay, Akos Varga-Szemes, David Jochheim, Moritz Baquet, Richard R Bayer, Ullrich Ebersberger, Sheldon E Litwin, Salvatore A Chiaramida, Ellen Hoffmann, U Joseph Schoepf
This study investigated the discriminatory value of quantitative atherosclerotic plaque markers derived from coronary computed tomography angiography (cCTA) in patients with first acute coronary syndrome (ACS) compared with patients with stable coronary artery disease (CAD). Forty patients (56.9 ± 9.3 years, 55% men) admitted with their first ACS and Framingham risk score-matched controls with stable CAD were retrospectively analyzed. All patients had undergone cCTA followed by invasive coronary angiography...
December 3, 2016: American Journal of Cardiology
https://www.readbyqxmd.com/read/28010676/influence-of-different-sitting-positions-on-healthy-infants-reaching-movements
#10
Erika Shirley Moreira da Silva, Gabriela Lopes Dos Santos, Ana Luiza Righetto Greco, Eloisa Tudella
The authors analyze the influence of different sitting positions (ring and flexion) on the reaching performance (spatiotemporal variables) of full-term infants 6 and 7 months old. In addition, they correlated level of trunk control, measured by the Segmental Assessment of Trunk Control, with performance during reaching. The different sitting positions only influenced the reaching movements of 6-month-old infants, who showed the best performance in the ring position. However, this influence was observed only for the trunk displacement, deceleration time, movement units, straightness index and mean velocity...
December 23, 2016: Journal of Motor Behavior
https://www.readbyqxmd.com/read/28007449/structural-studies-of-influenza-virus-rnps-by-electron-microscopy-indicate-molecular-contortions-within-np-supra-structures
#11
John R Gallagher, Udana Torian, Dustin M McCraw, Audray K Harris
Ribonucleoprotein (RNP) complexes of influenza viruses are composed of multiple copies of the viral nucleoprotein (NP) that can form filamentous supra-structures. RNPs package distinct viral genomic RNA segments of different lengths into pleomorphic influenza virions. RNPs also function in viral RNA transcription and replication. Different RNP segments have varying lengths, but all must be incorporated into virions during assembly and then released during viral entry for productive infection cycles. RNP structures serve varied functions in the viral replication cycle, therefore understanding their molecular organization and flexibility is essential to understanding these functions...
December 19, 2016: Journal of Structural Biology
https://www.readbyqxmd.com/read/27991790/electron-interference-in-hall-effect-measurements-on-gaas-inas-core-shell-nanowires
#12
Fabian Haas, Patrick Zellekens, Mihail Lepsa, Torsten Rieger, Detlev Grützmacher, Hans Lüth, Thomas Schäpers
We present low-temperature magnetotransport measurements on GaAs/InAs core/shell nanowires contacted by regular source-drain leads as well as laterally attached Hall contacts, which only touch parts of the nanowire sidewalls. Low-temperature measurements between source and drain contacts show typical phase coherent effects, such as universal conductance fluctuations in a magnetic field aligned perpendicularly to the nanowire axis as well as Aharonov-Bohm-type oscillations in a parallel aligned magnetic field...
December 19, 2016: Nano Letters
https://www.readbyqxmd.com/read/27984365/mid-term-results-of-a-single-intrastromal-corneal-ring-segment-for-mild-to-moderate-progressive-keratoconus
#13
Mohammad Miraftab, Hassan Hashemi, Farhad Hafezi, Soheila Asgari
PURPOSE: To study the mid-term effects of implanting a single intracorneal ring segment (ICRS) for treatment of mild to moderate progressive keratoconus. METHODS: This retrospective study was conducted on patients with progressive keratoconus treated with ICRS. The mean follow-up time was 15.2 ± 8.8 (range, 7-26) months before and 27.8 ± 7.5 (range, 18-35) months after surgery. Patients received no other treatment during this time. Of the cases studied, 59.1% had inferior keratoconus...
December 14, 2016: Cornea
https://www.readbyqxmd.com/read/27984362/efficacy-of-intrastromal-corneal-ring-segments-combined-with-flash-collagen-cross-linking-in-keratoconus
#14
Carlos H Gordillo, Juan C Grandin, Roger Zaldivar, Roberto Zaldivar, Adriana C Lotfi
PURPOSE: To evaluate outcomes, efficacy, and safety of intrastromal corneal ring segment implantation for treatment of keratoconus combined with collagen cross-linking (CXL) with 1-year follow-up. METHODS: One hundred nineteen eyes of 82 patients with keratoconus (37 females) underwent femtosecond (Intralase FS; Abbott Medical Optics, Inc) laser-assisted intrastromal corneal ring segment implantation (INTRASEG; Gamma Vision) with flash CXL (18 mW/cm for 5 minutes)...
December 14, 2016: Cornea
https://www.readbyqxmd.com/read/27974620/will-the-circle-be-unbroken-specific-mutations-in-the-yeast-sm-protein-ring-expose-a-requirement-for-assembly-factor-brr1-a-homolog-of-gemin2
#15
Beate Schwer, Allen J Roth, Stewart Shuman
A seven-subunit Sm protein ring assembles around specific U-rich RNA segments of the U1, U2, U4, and U5 snRNPs that direct pre-mRNA splicing. Using human snRNP crystal structures to guide mutagenesis in Saccharomyces cerevisiae, we gained new insights to structure-function relationships of the SmD1 and SmD2 subunits. Of sixteen conserved amino acids comprising their RNA-binding sites or inter-subunit interfaces, only Arg88 in SmD1 and Arg97 in SmD2 were essential for growth. Tests for genetic interactions with non-Sm splicing factors identified benign mutations of SmD1 (N37A, R88K, R93A) and SmD2 (R49A, N66A, R97K, D99A) that were synthetically lethal with null alleles of U2 snRNP subunits Lea1 and/or Msl1...
December 14, 2016: RNA
https://www.readbyqxmd.com/read/27958755/amphiphilic-ferrocene-containing-peg-block-copolymers-as-micellar-nanocarriers-and-smart-surfactants
#16
Arda Alkan, Sarah Wald, Benoit Louage, Bruno G De Geest, Katharina Landfester, Frederik R Wurm
An important and usually the only function of most surfactants in heterophase systems is stabilizing one phase in another, for example, droplets or particles in water. Surfactants with additional chemical or physical handles are promising in controlling the colloidal properties by external stimuli. The redox stimulus is an attractive feature; however, to date only a few ionic redox-responsive surfactants have been reported. Herein, the first nonionic and noncytotoxic ferrocene-containing block copolymers are prepared, carrying a hydrophilic poly(ethylene glycol) (PEG) chain and multiple ferrocenes in the hydrophobic segment...
December 29, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27933259/deformity-correction-and-extremity-lengthening-in-the-lower-leg-comparison-of-clinical-outcomes-with-two-external-surgical-procedures
#17
Eugen Reitenbach, Robert Rödl, Georg Gosheger, Björn Vogt, Frank Schiedel
OBJECTIVE: Distraction osteogenesis is a method of stimulating the growth of new bone tissue in order to lengthen the extremities or bridge resected bone defects. In addition to the now-established intramedullary procedures, two different fixator systems are in use. The present study investigated the classical Ilizarov ring fixator (IRF) and a hexapod to assess the precision of lower-leg lengthening and complications classified using the Paley criteria for problems, obstacles, and complications...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27933139/the-neuromuscular-system-of-pycnophyes-kielensis-kinorhyncha-allomalorhagida-investigated-by-confocal-laser-scanning-microscopy
#18
Andreas Altenburger
BACKGROUND: Kinorhynchs are ecdysozoan animals with a phylogenetic position close to priapulids and loriciferans. To understand the nature of segmentation within Kinorhyncha and to infer a probable ancestry of segmentation within the last common ancestor of Ecdysozoa, the musculature and the nervous system of the allomalorhagid kinorhynch Pycnophyes kielensis were investigated by use of immunohistochemistry, confocal laser scanning microscopy, and 3D reconstruction software. RESULTS: The kinorhynch body plan comprises 11 trunk segments...
2016: EvoDevo
https://www.readbyqxmd.com/read/27932442/contraceptive-sales-in-the-setting-of-the-zika-virus-epidemic
#19
Luis Bahamondes, Moazzam Ali, Ilza Monteiro, Arlete Fernandes
STUDY QUESTION: Has there been any influence of the Zika virus (ZIKV) outbreak on the sales of contraceptive methods in Brazil? SUMMARY ANSWER: Contraceptive sales in the 24 months of evaluation showed little variation and no significant change has been observed since the ZIKV outbreak. WHAT IS KNOWN ALREADY: Transmission of ZIKV is primarily by Aedes aegypti mosquitoes; however, sexual transmission has also been described. The association of several birth defects and the ZIKV infection during pregnancy has been established, and it was estimated in Bahia, Brazil that the infection rate could range from 10% to 80%...
January 2017: Human Reproduction
https://www.readbyqxmd.com/read/27930793/intracorneal-ring-segments-implantation-for-corneal-ectasia
#20
Natalia T Giacomin, Glauco R Mello, Carla S Medeiros, Alyin Kiliç, Cristine C Serpe, Hirlana G Almeida, Newton Kara-Junior, Marcony R Santhiago
PURPOSE: To provide an overview of the predictability, safety, and efficacy of intrastromal corneal ring segment (ICRS) implantation as a tool to improve visual acuity and its association with other techniques such as corneal collagen cross-linking (CXL), addressing biomechanical outcomes, models, surgical planning and technique, indications, contraindications, and complications in ectatic corneas. METHODS: Literature review. RESULTS: ICRSs have been used to regularize the corneal shape and reduce corneal astigmatism and higher order aberrations, improve visual acuity to acceptable limits, and delay, or eventually prevent, a corneal keratoplasty in keratoconic eyes...
December 1, 2016: Journal of Refractive Surgery
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