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https://www.readbyqxmd.com/read/28092855/lc-ms-ms-determination-of-d-mannose-in-human-serum-as-a-potential-cancer-biomarker
#1
Lyndsey White, Jing Ma, Su Liang, Beatriz Sanchez-Espiridion, Dong Liang
Several metabolites in human serum have been identified as potential cancer biomarkers for early detection. This study focuses on the LC-MS/MS method development and validation of d-mannose in human serum. Surrogate blank serum, coupled with stable isotope d-mannose-(13)C6, as internal standard, was used for generating standard curves ranging from 1 to 50μg/mL. Separation was achieved by an Agilent 1200 series HPLC equipped with a SUPELCOGELTM Pb, 6% Crosslinked column with HPLC water as a mobile phase at flow rate of 0...
December 28, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28092158/polyolefin-thermoplastics-for-multiple-shape-and-reversible-shape-memory
#2
Yuan Gao, Weifeng Liu, Shiping Zhu
This work reports the first pure hydrocarbon thermoplastic polyolefin material with reversible shape memory effect under stress-free or very small external loading condition. A thermoplastic ethylene/1-octene diblock copolymer with designed chain microstructure was synthesized. The polyolefin material performed not only the conventional one-way multi-shape memory effects, but also a two-way reversible shape memory effect (RSME). The elongation and contraction induced by oriented crystallization with heating was confirmed as the mechanism of RSME without chemical crosslinking...
January 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28091670/enhanced-temperature-effect-of-electrorheological-fluid-based-on-cross-linked-poly-ionic-liquid-particles-rheological-and-dielectric-relaxation-studies
#3
Yang Liu, Jinhua Yuan, Yuezhen Dong, Xiaopeng Zhao, Jianbo Yin
Recent research of using poly(ionic liquid) (PIL) particles as the dispersal phase has provided a new strategy to develop a high-performance anhydrous polyelectrolyte-based electrorheological (ER) fluid. However, the working temperature range of the ER fluid of PIL particles is still narrow due to an inherently low glass transfer temperature caused by the plasticization of polyatomic organic counter ions in PILs. In this paper, we develop a new ER system based on cross-linked PIL (C-PIL) particles and demonstrate that crosslinking with a suitable degree only slightly degrades the ER properties but significantly improves the working temperature range of the ER fluid of PIL particles...
January 16, 2017: Soft Matter
https://www.readbyqxmd.com/read/28088691/stability-effect-of-cholesterol-poly-acrylic-acid-in-a-stimuli-responsive-polymer-liposome-complex-obtained-from-soybean-lecithin-for-controlled-drug-delivery
#4
M G Simões, P Alves, Manuela Carvalheiro, P N Simões
The development of polymer-liposome complexes (PLCs), in particular for biomedical applications, has grown significantly in the last decades. The importance of these studies comes from the emerging need in finding intelligent controlled release systems, more predictable, effective and selective, for applications in several areas, such as treatment and/or diagnosis of cancer, neurological, dermatological, ophthalmic and orthopedic diseases, gene therapy, cosmetic treatments, and food engineering. This work reports the development and characterization of a pH sensitive system for controlled release based on PLCs...
January 4, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28088646/bone-microarchitecture-in-adolescent-boys-with-autism-spectrum-disorder
#5
Ann M Neumeyer, Natalia Cano Sokoloff, Erin McDonnell, Eric A Macklin, Christopher J McDougle, Madhusmita Misra
BACKGROUND: Boys with autism spectrum disorder (ASD) have lower areal bone mineral density (aBMD) than typically developing controls (TDC). Studies of volumetric BMD (vBMD) and bone microarchitecture provide information about fracture risk beyond that provided by aBMD but are currently lacking in ASD. OBJECTIVES: To assess ultradistal radius and distal tibia vBMD, bone microarchitecture and strength estimates in adolescent boys with ASD compared to TDC. DESIGN/METHODS: Cross-sectional study of 34 boys (16 ASD, 18 TDC) that assessed (i) aBMD at the whole body (WB), WB less head (WBLH), hip and spine using dual X-ray absorptiometry (DXA), (ii) vBMD and bone microarchitecture at the ultradistal radius and distal tibia using high-resolution peripheral quantitative CT (HRpQCT), and (iii) bone strength estimates (stiffness and failure load) using micro-finite element analysis (FEA)...
January 11, 2017: Bone
https://www.readbyqxmd.com/read/28088005/nicotine-hydrogen-tartrate-loaded-chitosan-nanoparticles-formulation-characterization-and-in-vitro-delivery-from-dry-powder-inhaler-formulation
#6
Hui Wang, Graeme George, Selena Bartlett, Changyou Gao, Nazrul Islam
This study reports the development of nanoparticles in the form of inhalable micro-aggregates of biodegradable chitosan (CS) loaded with nicotine hydrogen tartrate (NHT) for potential pulmonary delivery of nicotine from dry powder inhaler (DPI) formulations with prolonged release profile. The NHT-loaded CS particles were prepared using a water-in-oil emulsion crosslinking method. The prepared particles were characterized using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) for morphological studies; Zetasizer and Mastersizer were applied for particle size analysis...
January 11, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28087410/repair-of-8-oxog-a-mismatches-by-the-mutyh-glycosylase-mechanism-metals-medicine
#7
REVIEW
Douglas M Banda, Nicole N Nuñez, Michael A Burnside, Katie M Bradshaw, Sheila S David
Reactive oxygen and nitrogen species (RONS) may infringe on the passing of pristine genetic information by inducing DNA inter- and intra-strand crosslinks, protein-DNA crosslinks, and chemical alterations to the sugar or base moieties of DNA. 8-Oxo-7,8-dihydroguanine (8-oxoG) is one of the most prevalent DNA lesions formed by RONS and is repaired through the base excision repair (BER) pathway involving the DNA repair glycosylases OGG1 and MUTYH in eukaryotes. MUTYH removes adenine (A) from 8-oxoG:A mispairs, thus mitigating the potential of G:C to T:A transversion mutations from occurring in the genome...
January 10, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28087406/erythrocyte-membrane-coated-nanogel-for-combinatorial-antivirulence-and-responsive-antimicrobial-delivery-against-staphylococcus-aureus-infection
#8
Yue Zhang, Jianhua Zhang, Wangsong Chen, Pavimol Angsantikul, Kevin A Spiekermann, Ronnie H Fang, Weiwei Gao, Liangfang Zhang
We reported an erythrocyte membrane-coated nanogel (RBC-nanogel) system with combinatorial antivirulence and responsive antibiotic delivery for the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infection. RBC membrane was coated onto the nanogel via a membrane vesicle templated in situ gelation process, whereas the redox-responsiveness was achieved by using a disulfide bond-based crosslinker. We demonstrated that the RBC-nanogels effectively neutralized MRSA-associated toxins in extracellular environment and the toxin neutralization in turn promoted bacterial uptake by macrophages...
January 10, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28087384/epidermal-growth-factor-egf-fragment-guided-anticancer-theranostic-particles-for-ph-responsive-release-of-doxorubicin
#9
Myun Koo Kang, Wei Mao, Jun Bae Lee, Hyuk Sang Yoo
An EGF fragment and doxorubicin were chemically co-decorated on single magnetic nanoparticles for concomitant cancer targeting and treatment. Magnetic nanoparticles (MNPs) were prepared by the precipitation of ferric chloride hydrates in an ammonia solution and subsequent surface-functionalization with amines. The terminal thiol group of the EGF fragment (EGFfr) was first conjugated to surface amines of the MNPs using a heterofunctional crosslinker, and doxorubicin was sequentially conjugated to the MNPs via a hydrazone linker, where the degree of substation of the surface amines to EGF was varied from 1% to 40%...
January 10, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28087330/molecular-characterization-and-functional-analysis-of-a-salmon-louse-lepeophtheirus-salmonis-kr%C3%A3-yer-1838-heme-peroxidase-with-a-potential-role-in-extracellular-matrixes
#10
Aina-Cathrine Øvergård, Christiane Eichner, Frank Nilsen, Sussie Dalvin
Heme peroxidases are the most abundant type of peroxidase catalyzing a H2O2-dependent oxidation of a wide variety of substrates. They are involved in numerous processes like the innate immune response, hormone and prostaglandin synthesis and crosslinking of proteins within extracellular matrixes (ECM) as well as molecules within the cuticle and chorion of arthropods and nematodes. In the present study, a Lepeophtheirus salmonis heme peroxidase (LsHPX) 1 was characterized. Amino acids in the active site of heme peroxidases were conserved, and the predicted protein sequence showed the highest similarity to genes annotated as chorion peroxidases and genes suggested to be involved in cuticle hardening or adhesion...
January 10, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28087161/effect-of-acetabular-position-on-polyethylene-liner-wear-measured-using-simultaneous-biplanar-acquisition
#11
Prateek Goyal, James L Howard, Xunhua Yuan, Matthew G Teeter, Brent A Lanting
BACKGROUND: Studies that have previously examined the relationship between acetabular component inclination angle and polyethylene wear have shown increased wear of conventional polyethylene with high inclination angles. To date, there are no long-term in vivo studies examining the correlation between cup position and polyethylene wear with highly crosslinked polyethylene. METHODS: An institutional arthroplasty database was used to identify patients who had metal-on-highly crosslinked polyethylene primary total hip arthroplasty using the same component design with a minimum follow-up of 10 years...
December 14, 2016: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28081493/optical-and-vibrational-properties-of-phosphorylcholine-based-contact-lenses-experimental-and-theoretical-investigations
#12
Katarzyna Filipecka, Rafał Miedziński, Maciej Sitarz, Jacek Filipecki, Małgorzata Makowska-Janusik
The Raman, MIR and UV-vis spectroscopy have been used to characterize Omafilcon A material constructing the one of the Proclear family contact lenses. The Omafilcon A is hydrogel material composed of 2-hydroxyethyl methacrylate (HEMA) and 2-methacryloyloxyethyl phosphorylcholine (PC) polymers crosslinked with ethyleneglycol dimethacrylate (EGDMA). Vibrational and electronic properties of the Omafilcon A material were also investigated by quantum chemical calculations. Experimentally obtained Raman, MIR and optical spectra were compared to the theoretical ones calculated applying RHF and DFT methodology...
January 5, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28081456/robust-tough-and-anti-fatigue-cationic-latex-composite-hydrogels-based-on-dual-physically-cross-linked-networks
#13
Song Gu, Lijie Duan, Xiuyan Ren, Guang Hui Gao
Dual physically cross-linked hydrogels, which are triggered by cationic latexes as hydrophobic association and ionic crosslinking centers, were easily fabricated via a one-pot in situ polymerization method. First, the hydrophobic alkyl chains of hydrophobic monomers are adsorbed on the surface of latex microspheres and stabilized in the presence of surfactants, forming hydrophobic association centers as the first physical crosslinking points. Meanwhile, the anionic sulfate radicals dissociated by persulphate are attracted towards the cationic molecular chains of latex microspheres through ionic interactions, forming the secondary physical crosslinking centers, and initiate the copolymerization between acrylamide and hydrophobic vinyl monomers...
January 4, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28079590/the-evaluation-of-corneal-fragility-after-uva-riboflavin-crosslinking
#14
Zhiwei Li, Yumeng Wang, Yanyun Xu, Vishal Jhanji, Chunxiao Zhang, Guoying Mu
PURPOSE: To evaluate the fragility of cornea after UVA/riboflavin crosslinking (CXL). METHODS: Sixty New Zealand rabbits received UVA/riboflavin crosslinking treatment (wavelength 365 nm, irradiance 3.0 mW/cm, and total dose 5.4 J/cm) on right eyes. Animals were sacrificed before and immediately after treatment (day 0), day 1, 3, 7, and 28 after treatment. A 4×10 mm corneal strip for biomechanical evaluation was harvested after sacrifice. The corneal fragility was evaluated by measurement of elongation rate, whereby the elongation rate equals elongation length/baseline length...
January 10, 2017: Eye & Contact Lens
https://www.readbyqxmd.com/read/28079290/clamped-hybridization-chain-reactions-for-the-self-assembly-of-patterned-dna-hydrogels
#15
Jianbang Wang, Jie Chao, Huajie Liu, Shao Su, Lianhui Wang, Wei Huang, Itamar Willner, Chunhai Fan
DNA hydrogels hold great potential for biological and biomedical applications owing to their programmable nature and macroscopic sizes. However, most previous studies involve spontaneous and homogenous gelation procedures in solution, which often lack precise control. A clamped hybridization chain reaction (C-HCR)-based strategy has been developed to guide DNA self-assembly to form macroscopic hydrogels. Analogous to catalysts in chemical synthesis or seeds in crystal growth, we introduced DNA initiators to induce the gelation process, including crosslinked self-assembly and clamped hybridization in three dimensions with spatial and temporal control...
January 12, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28079002/neurotoxicity-associated-with-platinum-based-anti-cancer-agents-what-are-the-implications-of-copper-transporters
#16
Vanesa Stojanovska, Rachel McQuade, Emma Rybalka, Kulmira Nurgali
Platinum-based anti-cancer agents, which include cisplatin, carboplatin and oxaliplatin, are an important class of drugs used in clinical setting to treat a variety of cancers. The cytotoxic efficacy of these drugs is mediated by the formation of interstrand and intrastrand crosslinks, or platinum adducts on nuclear DNA. There is also evidence demonstrating that mitochondrial DNA is susceptible to platinum-adduct damage in dorsal root ganglia neurons. Although all platinum-based agents form similar DNA adducts, they are quite different in terms of activation, systemic toxicity and tolerance...
January 11, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28078347/alterations-of-the-platelet-proteome-in-type-i-glanzmann-thrombasthenia-caused-by-different-homozygous-delg-frameshift-mutations-in-itga2b
#17
Stefan Loroch, Katharina Trabold, Stepan Gambaryan, Cora Reiß, Kathrin Schwierczek, Ingrid Fleming, Albert Sickmann, Wolfgang Behnisch, Barbara Zieger, René P Zahedi, Ulrich Walter, Kerstin Jurk
Glanzmann thrombasthenia (GT) is one of the best characterized inherited platelet function disorders but global platelet proteome has not been determined in these patients. We investigated the proteome and function of platelets from two patients with type I GT, caused by different homozygous ITGA2b mutations, from family members and unrelated controls. The global proteome of highly purified washed platelets was quantified by liquid chromatography-mass spectrometry (LC-MS) and targeted MS-methods. Platelet function was analysed by flow cytometry, light transmission aggregometry and flow-based assays...
January 12, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28078343/differential-effects-of-silver-and-iron-oxide-nanoparticles-on-iapp-amyloid-aggregation
#18
Miaoyi Wang, Aleksandr Kakinen, Emily H Pilkington, Thomas P Davis, Pu Chun Ke
Recent studies have shown promise on the use of small molecules and nanoparticles (NPs) for the inhibition of protein aggregation, a hallmark of neurodegenerative diseases and type 2 diabetes (T2D). Towards this end here we show the differential effects of silver and iron oxide nanoparticles (AgNPs and IONPs) on the mesoscopic properties of human islet amyloid polypeptide (IAPP) aggregation associated with T2D. Both citrate- and branched polyethyleneimine-coated AgNPs (c-AgNPs, bPEI-AgNPs) inhibited IAPP aggregation at 500 μg mL(-1), likely through electrostatic attraction and sequestering of IAPP monomers from fibrillation...
January 12, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28078338/dual-physically-crosslinked-double-network-hydrogels-with-high-toughness-and-self-healing-properties
#19
Xuefeng Li, Qian Yang, Youjiao Zhao, Shijun Long, Jie Zheng
Toughness and self-healing properties are desirable characteristics in engineered hydrogels used for many practical applications. However, it is still challenging to develop hydrogels exhibiting both of these attractive properties in a single material. In this work, we present the fabrication of fully physically-linked Agar/PAAc-Fe(3+) DN gels. These hydrogels exhibited dual physical crosslinking through a hydrogen bonded crosslinked agar network firstly, and a physically linked PAAc-Fe(3+) network via Fe(3+) coordination interactions secondly...
January 12, 2017: Soft Matter
https://www.readbyqxmd.com/read/28077981/dna-polymerase-kappa-protects-human-cells-against-mmc-induced-genotoxicity-through-error-free-translesion-dna-synthesis
#20
Yuki Kanemaru, Tetsuya Suzuki, Akira Sassa, Kyomu Matsumoto, Noritaka Adachi, Masamitsu Honma, Satoshi Numazawa, Takehiko Nohmi
BACKGROUND: Interactions between genes and environment are critical factors for causing cancer in humans. The genotoxicity of environmental chemicals can be enhanced via the modulation of susceptible genes in host human cells. DNA polymerase kappa (Pol κ) is a specialized DNA polymerase that plays an important role in DNA damage tolerance through translesion DNA synthesis. To better understand the protective roles of Pol κ, we previously engineered two human cell lines either deficient in expression of Pol κ (KO) or expressing catalytically dead Pol κ (CD) in Nalm-6-MSH+ cells and examined cytotoxic sensitivity against various genotoxins...
2017: Genes and Environment: the Official Journal of the Japanese Environmental Mutagen Society
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