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https://www.readbyqxmd.com/read/29786885/automated-buildup-of-biomimetic-films-in-cell-culture-microplates-for-high-throughput-screening-of-cellular-behaviors
#1
Paul Machillot, Catarina Quintal, Fabien Dalonneau, Loic Hermant, Pauline Monnot, Kelsey Matthews, Vincent Fitzpatrick, Jie Liu, Isabelle Pignot-Paintrand, Catherine Picart
An automatic method is established for layer-by-layer (LbL) assembly of biomimetic coatings in cell culture microplates using a commercial liquid-handling robot. Highly homogeneous thin films are formed at the bottom of each microwell. The LbL film-coated microplates are compatible with common cellular assays, using microplate readers and automated microscopes. Cellular adhesion is screened on crosslinked and peptide-functionalized LbL films and stem cell differentiation in response to increasing doses of bone morphogenetic proteins (2, 4, 7, 9)...
May 22, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29785178/functionally-graded-bone-and-tendon-like-polyurethane-for-rotator-cuff-repair
#2
Dai Fei Elmer Ker, Dan Wang, Anthony William Behn, Evelyna Tsi Hsin Wang, Xu Zhang, Benjamin Yamin Zhou, Ángel Enrique Mercado-Pagán, Sungwoo Kim, John Kleimeyer, Burhan Gharaibeh, Yaser Shanjani, Drew Nelson, Marc Safran, Emilie Cheung, Phil Campbell, Yunzhi Peter Yang
Critical considerations in engineering biomaterials for rotator cuff repair include bone-tendon-like mechanical properties to support physiological loading and biophysicochemical attributes that stabilize the repair site over the long-term. In this study, UV-crosslinkable polyurethane based on quadrol (Q), hexamethylene diisocyante (H), and methacrylic anhydride (M; QHM polymers), which are free of solvent, catalyst, and photoinitiator, is developed. Mechanical characterization studies demonstrate that QHM polymers possesses phototunable bone- and tendon-like tensile and compressive properties (12-74 MPa tensile strength, 0...
May 2018: Advanced Functional Materials
https://www.readbyqxmd.com/read/29785079/decreasing-recurrent-bowel-obstructions-improving-quality-of-life-with-physiotherapy-controlled-study
#3
Amanda D Rice, Kimberley Patterson, Evette D Reed, Belinda F Wurn, Kristen Robles, Bernhard Klingenberg, Leonard B Weinstock, Janey Sa Pratt, C Richard King, Lawrence J Wurn
AIM: To compare (1) quality of life and (2) rate of recurrent small bowel obstructions (SBO) for patients treated with novel manual physiotherapy vs no treatment. METHODS: One hundred and three subjects (age 19-89) with a history of recurrent adhesive SBO were treated with a manual physiotherapy called the Clear Passage Approach (CPA) which focused on decreasing adhesive crosslinking in abdominopelvic viscera. Pre- and post-therapy data measured recurring obstructions and quality of life, using a validated test sent 90 d after therapy...
May 21, 2018: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29784551/residual-byproducts-of-peroxide-crosslinked-vitamin-e-blended-ultrahigh-molecular-weight-polyethylene
#4
David A Bichara, Caitlin C O'Brien, Brinda N Doshi, Gunnlaugur P Nielsen, Ebru Oral, Orhun K Muratoglu
BACKGROUND: Wear resistance of ultrahigh molecular weight polyethylene (UHMWPE) is improved via ionizing radiation crosslinking and subsequent high temperature melting for improved toughness. Our group has previously reported that crosslinking can also be achieved chemically using organic peroxides. However, volatile peroxide byproducts are generated during consolidation. The purpose of this study was to quantify elution of volatile peroxide byproducts from UHMWPE before and after in vivo implantation, and to determine their effects on local tissues...
March 13, 2018: Journal of Arthroplasty
https://www.readbyqxmd.com/read/29784386/fabrication-of-powdery-polymer-aerogel-as-the-stationary-phase-for-high-resolution-gas-chromatographic-separation
#5
Juan Zheng, Cuiming Lu, Junlong Huang, Luyi Chen, Chuyi Ni, Xintong Xie, Fang Zhu, Dingcai Wu, Gangfeng Ouyang
Novel powdery polymer aerogel (PPA) prepared via the (micro)emulsion polymerization and the following hyper crosslinking reaction was fabricated as stationary phase of capillary column for the first time. Due to its powdery morphology, unique 3D nano-network structure, high surface area and good thermostability, the PPA-coated capillary column demonstrated high-resolution chromatographic separation towards nonpolar and weakly polar organic compounds, including benzene series, n-alkanes, ketone mixtures and trichlorobenzenes...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29783776/layer-by-layer-heparinization-of-the-cell-surface-by-using-heparin-binding-peptide-functionalized-human-serum-albumin
#6
Guowei Song, Yaning Hu, Yusheng Liu, Rui Jiang
Layer-by-layer heparinization of therapeutic cells prior to transplantation is an effective way to inhibit the instant blood-mediated inflammatory reactions (IBMIRs), which are the major cause of early cell graft loss during post-transplantation. Here, a conjugate of heparin-binding peptide (HBP) and human serum albumin (HSA), HBP-HSA, was synthesized by using heterobifunctional crosslinker. After the first heparin layer was coated on human umbilical vein endothelial cells (HUVECs) by means of the HBP-polyethylene glycol-phospholipid conjugate, HBP-HSA and heparin were then applied to the cell surface sequentially to form multiple layers...
May 20, 2018: Materials
https://www.readbyqxmd.com/read/29782703/spatial-micropatterning-of-growth-factors-in-3d-hydrogels-for-location-specific-regulation-of-cellular-behaviors
#7
Oju Jeon, Keewon Lee, Eben Alsberg
Growth factors are potent stimuli for regulating cell function in tissue engineering strategies, but spatially patterning their presentation in 3D in a facile manner using a single material is challenging. Micropatterning is an attractive tool to modulate the cellular microenvironment with various biochemical and physical cues and study their effects on stem cell behaviors. Implementing heparin's ability to immobilize growth factors, dual-crosslinkable alginate hydrogels are micropatterned in 3D with photocrosslinkable heparin substrates with various geometries and micropattern sizes, and their capability to establish 3D micropatterns of growth factors within the hydrogels is confirmed...
May 21, 2018: Small
https://www.readbyqxmd.com/read/29782701/tgf-%C3%AE-1-modified-hyaluronic-acid-poly-glycidol-hydrogels-for-chondrogenic-differentiation-of-human-mesenchymal-stromal-cells
#8
Thomas Böck, Verena Schill, Martin Krähnke, Andre F Steinert, Jörg Tessmar, Torsten Blunk, Jürgen Groll
In cartilage regeneration, the biomimetic functionalization of hydrogels with growth factors is a promising approach to improve the in vivo performance and furthermore the clinical potential of these materials. In order to achieve this without compromising network properties, multifunctional linear poly(glycidol) acrylate (PG-Acr) is synthesized and utilized as crosslinker for hydrogel formation with thiol-functionalized hyaluronic acid via Michael-type addition. As proof-of-principle for a bioactivation, transforming growth factor-beta 1 (TGF-β1) is covalently bound to PG-Acr via Traut's reagent which does not compromise the hydrogel gelation and swelling behavior...
May 21, 2018: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29782154/miclip-mapseq-a-substrate-identification-approach-for-radical-sam-rna-methylating-enzymes
#9
Vanja Stojković, Tongyue Chu, Gabriel Therizols, David E Weinberg, Danica Galonić Fujimori
Although present across bacteria, the large family of radical SAM RNA methylating enzymes is largely uncharacterized. Escherichia coli RlmN, the founding member of the family, methylates an adenosine in 23S rRNA and several tRNAs to yield 2-methyladenosine (m2A). However, varied RNA substrate specificity among RlmN enzymes, combined with the ability of certain family members to generate 8-methyladenosine (m8A), makes functional predictions across this family challenging. Here, we present a method for unbiased substrate identification that exploits highly efficient, mechanism-based crosslinking between the enzyme and its RNA substrates...
May 21, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29780993/ideal-reversible-polymer-networks
#10
German Alberto Parada, Xuanhe Zhao
In this article we introduce the concept of ideal reversible polymer networks, which have well-controlled polymer network structures similar to ideal covalent polymer networks but exhibit viscoelastic behaviors due to the presence of reversible crosslinks. We first present a theory to describe the mechanical properties of ideal reversible polymer networks. Because short polymer chains of equal length are used to construct the network, there are no chain entanglements and the chains' Rouse relaxation time is much shorter than the reversible crosslinks' characteristic time...
May 21, 2018: Soft Matter
https://www.readbyqxmd.com/read/29780985/the-correlation-between-gelatin-macroscale-differences-and-nanoparticle-properties-providing-insight-into-biopolymer-variability
#11
André T Stevenson, Danny J Jankus, Max A Tarshis, Abby R Whittington
From therapeutic delivery to sustainable packaging, manipulation of biopolymers into nanostructures imparts biocompatibility to numerous materials with minimal environmental pollution during processing. While biopolymers are appealing natural based materials, the lack of nanoparticle (NP) physicochemical consistency has decreased their nanoscale translation into actual products. Insights regarding the macroscale and nanoscale property variation of gelatin, one of the most common biopolymers already utilized in its bulk form, are presented...
May 21, 2018: Nanoscale
https://www.readbyqxmd.com/read/29779665/assaying-oxidative-coupling-activity-of-cyp450-enzymes
#12
Vinayak Agarwal
Cytochrome P450 (CYP450) enzymes are ubiquitous catalysts in natural product biosynthetic schemes where they catalyze numerous different transformations using radical intermediates. In this protocol, we describe procedures to assay the activity of a marine bacterial CYP450 enzyme Bmp7 which catalyzes the oxidative radical coupling of polyhalogenated aromatic substrates. The broad substrate tolerance of Bmp7, together with rearrangements of the aryl radical intermediates leads to a large number of products to be generated by the enzymatic action of Bmp7...
2018: Methods in Enzymology
https://www.readbyqxmd.com/read/29779627/in-vitro-assessment-of-ribose-modified-two-step-etch-and-rinse-dentine-adhesive
#13
U Daood, J K H Tsoi, P Neelakantan, J P Matinlinna, H A K Omar, M Al-Nabulsi, A S Fawzy
OBJECTIVE: Collagen fibrils aid in anchoring resin composite restorations to the dentine substrate. The aim of the study was to investigate effect of non-enzymatic glycation on bond strength and durability of demineralized dentine specimens in a modified two-step etch-and-rinse dentine adhesive. METHODS: Dentine surfaces were etched with 37% phosphoric acid, bonded with respective in vitro ethanol and acetone adhesives modified with (m/m, 0, 1%, 2% and 3% ribose), restored with restorative composite-resin, and sectioned into resin-dentine slabs and beams to be stored for 24h or 12 months in artificial saliva...
May 17, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29779246/regulation-of-plasminogen-activation-on-cell-surfaces-and-fibrin
#14
REVIEW
Tetsumei Urano, Francis J Castellino, Yuko Suzuki
The fibrinolytic system dissolves fibrin and maintains vascular patency. Recent advances in imaging analyses allowed visualization of the spatiotemporal regulatory mechanism of fibrinolysis, as well as its regulation by other plasma haemostasis cofactors. Vascular endothelial cells (VECs) retain tissue-type plasminogen activator (tPA) after secretion and maintain high plasminogen (plg) activation potential on their surfaces. As in plasma, the serpin, plasminogen activator inhibitor type 1 (PAI-1), regulates fibrinolytic potential via inhibition of the VEC surface-bound plg activator, tPA...
May 20, 2018: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/29779110/translocation-of-oligonucleotide-oligosaccharide-complexes-into-cells-of-the-brain
#15
N N Dygalo, T S Kalinina, D A Lanshakov
Irradiation of a mixture of oligonucleotides with dextran resulted in the formation of a complex that is detected by a decelerated migration of an irradiated sample in electrophoretic gel compared to a non-irradiated one. When injected into the brain of neonatal rats, the formed complex penetrated into the cells 3 times easier compared to the original oligonucleotide, thus indicating that the use of radiation crosslinking of oligonucleotides with oligosaccharides is promising to enhance the efficiency of delivery of gene-targeted oligonucleotide drugs into cells...
March 2018: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29778960/rapid-colorimetric-detection-of-p53-protein-function-using-dna-gold-nanoconjugates-with-applications-for-drug-discovery-and-cancer-diagnostics
#16
Enock Assah, Walter Goh, Xin Ting Zheng, Ting Xiang Lim, Jun Li, David Lane, Farid Ghadessy, Yen Nee Tan
The tumor suppressor protein p53 plays a central role in preventing cancer through interaction with DNA response elements (REs) to regulate target gene expression in cells. Due to its significance in cancer biology, relentless efforts have been directed toward understanding p53-DNA interactions for the development of cancer therapeutics and diagnostics. In this paper, we report a rapid, label-free and versatile colorimetric assay to detect wildtype p53 DNA-binding function in complex solutions. The assay design is based on a concept that alters interparticle-distances between RE-AuNPs from a crosslinking effect induced through tetramerization of wildtype p53 protein (p53-WT) upon binding to canonical DNA motifs modified on gold nanoparticles (RE-AuNPs)...
May 5, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29778454/influence-of-the-beam-profile-crosslinking-setting-on-changes-in-corneal-topography-and-tomography-in-progressive-keratoconus-preliminary-results
#17
Robert Herber, Kathleen S Kunert, Věra Veliká, Eberhard Spoerl, Lutz E Pillunat, Frederik Raiskup
PURPOSE: To evaluate the effect of accelerated corneal crosslinking (CXL) with a "higher peripheral intensity" profile setting compared with a standard "top hat" profile setting performed using different CXL systems. SETTING: Department of Ophthalmology, University Hospital Carl Gustav Carus, Dresden, Germany. DESIGN: Retrospective case series. METHODS: Eyes from patients with progressive keratoconus were included...
May 16, 2018: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/29778107/photorefractive-intrastromal-corneal-crosslinking-for-treatment-of-myopic-refractive-error-findings-from-12-month-prospective-study-using-an-epithelium-off-protocol
#18
Matthias Elling, Inga Kersten-Gomez, H Burkhard Dick
PURPOSE: To assess the long-term effectiveness and safety of photorefractive intrastromal corneal crosslinking (CXL) in patients with low myopia. SETTING: Ruhr University Eye Hospital, Bochum, Germany. DESIGN: Prospective case series. METHODS: Healthy eyes with myopia were treated with photorefractive intrastromal CXL using the Mosaic System. Riboflavin 0.1% solution (VibeX Rapid) was topically administered, followed by 365 nm ultraviolet-A (30 mW/cm2 ) irradiance...
April 2018: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/29777818/influence-of-degree-of-substitution-and-folic-acid-coinitiator-on-pullulan-hema-hydrogel-properties-crosslinked-under-visible-light-initiating-system
#19
Elbadawy A Kamoun, M A Abu-Seaid, A S Doma, Henning Menzel, Xin Chen
Hydroxyethyl methacrylate-derivative pullulan (pullulan-HEMA) was synthesized by activation of HEMA followed by catalyzed coupling reaction for adjusting the degree of substitution (DS) of copolymer. Pullulan-HEMA was photocrosslinked using new three-components photoinitiating system composed of carboxylated camphorquinone-folic acid-iodonium salt under visible light. Folic acid was employed as new coinitiator for improving the entire hydrogel properties and avoiding harms of traditional used tertiary amine coinitiators...
May 16, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29777678/uva-riboflavin-collagen-crosslinking-stiffening-effects-on-anterior-and-posterior-corneal-flaps
#20
Hamed Hatami-Marbini, Sandeep M Jayaram
The UVA/riboflavin collagen crosslinking (CXL) is one of the treatment procedure for stopping the progression of keratoconus. The inclusion criterion for this procedure is a minimum corneal thickness of 400 μm, which is not often met in patients with advanced keratoconus. Preoperatively swelling of thin corneas was shown to stabilize the keratectasia without any postoperative endothelial damage. Recently, we have shown that swelling porcine corneas prior to the CXL treatment had no significant effect on the resulting improvement in their tensile properties...
May 16, 2018: Experimental Eye Research
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