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Pulp regeneration

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https://www.readbyqxmd.com/read/29341308/stiffness-regulates-the-proliferation-and-osteogenic-odontogenic-differentiation-of-human-dental-pulp-stem-cells-via-the-wnt-signalling-pathway
#1
Nanxin Liu, Mi Zhou, Qi Zhang, Tao Zhang, Taoran Tian, Quanquan Ma, Changyue Xue, Shiyu Lin, Xiaoxiao Cai
OBJECTIVES: Researches showed that stiffness of the extracellular matrix can affect the differentiation of many stem cells. Dental pulp stem cells (DPSCs) are a promising type of adult stem cell. However, we know little about whether and how the behaviour of DPSCs is influenced by stiffness. MATERIALS AND METHODS: We carried out a study that cultured DPSCs on tunable elasticity polydimethylsiloxane substrates to investigate the influence on morphology, proliferation, osteogenic/odontogenic differentiation and its possible mechanism...
January 17, 2018: Cell Proliferation
https://www.readbyqxmd.com/read/29316874/liposomal-delivery-of-demineralised-dentine-matrix-for-dental-tissue-regeneration
#2
Genevieve Elizabeth Melling, John Colombo, Steven J Avery, Wayne Ayre, Samuel L Evans, Rachel Jane Waddington, Alastair James Sloan
Current dental restorations have short longevity, consequently there is a need for novel tissue engineering strategies that aim to regenerate the dentine-pulp complex. Dentine matrix contains a myriad of bioactive growth factors and extracellular matrix proteins associated with the recruitment, proliferation and differentiation of dental pulp progenitor cells. Here, we show that demineralised dentine matrix (DDM), from non-carious dentine, can be encapsulated into liposomes for delivery to dental tissue to promote regeneration...
January 9, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29313241/cd146-positive-human-dental-pulp-stem-cells-promote-regeneration-of-dentin-pulp-like-structures
#3
Mikiko Matsui, Tomoko Kobayashi, Takeo W Tsutsui
CD146 and STRO-1 are endothelial biomarkers that are co-expressed on the cellular membranes of blood vessels within human dental pulp tissue. This study characterized the percentage of dentin-like structures produced by CD146-positive (CD146+) human dental pulp stem cells (DPSCs), compared with their CD146-negative (CD146-) counterparts. DPSC populations were enriched using magnetic-activated cell sorting (MACS), yielding CD146+ and CD146- cells, as well as mixtures composed of 25% CD146+ cells and 75% CD146- cells (CD146+/-)...
January 8, 2018: Human Cell
https://www.readbyqxmd.com/read/29306536/orthodontic-movement-after-regenerative-endodontic-procedure-case-report-and-long-term-observations
#4
Antonis Chaniotis
INTRODUCTION: Although regenerative treatment approaches in teeth with incomplete root formation and pulp necrosis have become part of the suggested therapeutic endodontic spectrum, little is known about the effect of orthodontic movement in the tissue that has been regenerated. Furthermore, as the number of adults undergoing orthodontic treatment increases, there is an increasing need to investigate the changes that these tissues may undergo during orthodontic movement. Here we describe the alterations observed after the application of orthodontic forces in a case of an apically root-fractured necrotic immature root that had been managed with regenerative endodontic procedures in the past...
January 3, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29298406/a-miniature-swine-model-for-stem-cell-based-de-novo-regeneration-of-dental-pulp-and-dentin-like-tissue
#5
Xiaofei Zhu, Jie Liu, Zongdong Yu, Chao-An Chen, Hacer Aksel, Adham A Azim, George T-J Huang
The goal of this study was to establish mini-swine as a large animal model for stem cell-based pulp regeneration studies. Swine dental pulp stem cells (sDPSCs) were isolated from mini-swine and characterized in vitro. For in vivo studies, we first employed both ectopic and semi-orthotopic study models using severe combined immunodeficiency mice. One is hydroxyapatite-tricalcium phosphate (HA/TCP) model for pulp-dentin complex formation, and the other is tooth fragment model for complete pulp regeneration with new dentin depositing along the canal walls...
January 3, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29284978/successful-surgical-management-of-palatogingival-groove-using-platelet-rich-fibrin-and-guided-tissue-regeneration-a-novel-approach
#6
J V Karunakaran, Susan Mathew Fenn, N Jayaprakash, N Ragavendran
Palatogingival groove also known as radicularlingual groove is a developmental anomaly involving the lingual surface of the maxillary incisors. They are inconspicuous, funnel-shaped, extend for varying distances on root surface and occur due to infolding of the hertwigs epithelial root sheath. This encourages adherence of microorganisms and plaque to levels significant for pathological changes resulting in endodontic and periodontal lesions. The variations in anatomy of the tooth as a cause of pulp necrosis in teeth of anterior maxillary segment should be considered by the clinician when other etiological factors are ruled out...
November 2017: Journal of Pharmacy & Bioallied Sciences
https://www.readbyqxmd.com/read/29275854/edta-treatment-for-sodium-hypochlorite-treated-dentin-recovers-disturbed-attachment-and-induces-differentiation-of-mouse-dental-papilla-cells
#7
Kentaro Hashimoto, Nobuyuki Kawashima, Shizuko Ichinose, Keisuke Nara, Sonoko Noda, Takashi Okiji
INTRODUCTION: The disturbance of cellular attachment to dentin by sodium hypochlorite (NaOCl) may hamper pulp tissue regeneration. The aims of this study were to examine the recovering effect of EDTA on the attachment/differentiation of stemlike cells and to address the mechanisms of EDTA-induced recovery under the hypothesis that attachment to the exposed dentin matrix and the subsequent activation of integrin/phosphatidylinositol 3-kinase (PI3K) signaling play a crucial role. METHODS: Mouse dental papilla (MDP) cells were cultured on bovine dentin disks treated with NaOCl (0%, 1...
December 21, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/29260758/-regeneration-of-dental-pulp-tissue-using-pulpal-autologous-mesenchymal-stem-cells-and-platelet-rich-plasma
#8
A A Kulakov, D V Goldshtein, E K Krechina, T B Bukharova, A V Volkov, A K Gadzhiev
Regeneration of pulp and dentin could be important in operative dentistry as a method to save teeth. Currently cell population from dental pulp of deciduous and permanent teeth of humans and laboratory animals are isolated and characterized. The paper presents a study on pulp regeneration using autologous mesenchymal stromal cells from pulp of molars in combination with fibrin clot, transplanted in pulp chamber of miniature pigs after pulp removal. The results proved that transplantation of autologous multipotent stromal cells of dental pulp in combination with autologous platelet-rich plasma in pulp chamber of miniature pigs after pulp removal leads to pulp restoration and reparative dentinogenesis with dentinal bridge formation on the 30th day...
2017: Stomatologii︠a︡
https://www.readbyqxmd.com/read/29259707/isolation-of-dental-pulp-stem-cells-with-high-osteogenic-potential
#9
REVIEW
Takazumi Yasui, Yo Mabuchi, Satoru Morikawa, Katsuhiro Onizawa, Chihiro Akazawa, Taneaki Nakagawa, Hideyuki Okano, Yumi Matsuzaki
Dental pulp stem cells/progenitor cells (DPSCs) can be easily obtained and can have excellent proliferative and mineralization potentials. Therefore, many studies have investigated the isolation and bone formation of DPSCs. In most previous reports, human DPSCs were traditionally isolated by exploiting their ability to adhere to plastic tissue culture dishes. DPSCs isolated by plastic adherence are frequently contaminated by other cells, which limits the ability to investigate their basic biology and regenerative properties...
2017: Inflammation and Regeneration
https://www.readbyqxmd.com/read/29246377/dimethyloxalylglycine-embedded-poly-%C3%AE%C2%B5-caprolactone-fiber-meshes-promote-odontoblastic-differentiation-of-human-dental-pulp-derived-cells
#10
Yeon-Jee Yoo, Joung-Hwan Oh, Qiankun Zhang, WooCheol Lee, Kyung Mi Woo
INTRODUCTION: The in vivo effect of prolyl hydroxylase inhibitors on the regeneration of the pulp-dentin complex is unclear. The purpose of this study was to investigate the effect of dimethyloxalylglycine (DMOG)-embedded poly(ε-caprolactone) fiber (PCLF/DMOG) on odontoblastic differentiation of human dental pulp-derived cells (hDPCs) by transplantation of the dentin slice model. METHODS: The hDPCs were seeded onto electrospun PCLF and PCLF/DMOG in dentin slices and then transplanted into nude mice...
January 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29244761/study-of-separation-and-fouling-of-reverse-osmosis-membranes-during-model-hydrolysate-solution-filtration
#11
Olumoye Ajao, Mohamed Rahni, Mariya Marinova, Hassan Chadjaa, Oumarou Savadogo
Prehydrolysate, a dilute solution consisting mainly of pentoses, hexoses, and lesser quantities of organic acids, furfural and phenolics, is generated in the Kraft dissolving pulp process. An obstacle facing the valorization of the solution in hemicellulose biorefineries, by conversion of the sugars into bioproducts such as furfural, is the low sugar concentration. Membrane filtration is typically proposed in several hemicellulose based biorefineries for concentrating the solution, although they are usually generated using different wood species, pretreatment methods, and operating conditions...
December 15, 2017: Membranes
https://www.readbyqxmd.com/read/29221472/odontoblast-like-differentiation-and-mineral-formation-of-pulpsphere-derived-cells-on-human-root-canal-dentin-in-vitro
#12
Jörg Neunzehn, Sandra Pötschke, Christian Hannig, Hans-Peter Wiesmann, Marie-Theres Weber
BACKGROUND: The revitalization or regeneration of the dental pulp is a preferable goal in current endodontic research. In this study, human dental pulp cell (DPC) spheres were applied to human root canal samples to evaluate their potential adoption for physiological tissue-like regeneration of the dental root canal by odontoblastic differentiation as well as cell-induced mineral formation. METHODS: DPC were cultivated into three-dimensional cell spheres and seeded on human root canal specimens...
December 8, 2017: Head & Face Medicine
https://www.readbyqxmd.com/read/29215832/-activation-of-regeneration-of-red-and-white-pulp-of-the-spleen-after-the-combined-transplantation-of-hsc-and-mscs-in-terms-of-exposure-to-ionizing-radiation
#13
I Yu Maklakova, D Yu Grebnev, A P Yastrebov
The purpose of this work was to study the effect of combined transplantation of multipotent mesenchymal stromal (MSCS) and hematopoietic stem cells (HSCs) isolated from the placenta, on the regeneration of white and red pulp of the spleen under physiological conditions and in conditions of exposure to ionizing radiation. Methods. The experiments were performed with laboratory mice-males. We studied the influence of ionizing radiation dose of 4.0 Gy. Animals of the experimental group were intravenously infused into MMSC and GSK respectively at a dose of 6 million cells/kg and 330 thousand cells/kg, suspended in 0...
April 2017: Patologicheskaia Fiziologiia i èksperimental'naia Terapiia
https://www.readbyqxmd.com/read/29215170/synthetic-light-curable-polymeric-materials-provide-a-supportive-niche-for-dental-pulp-stem-cells
#14
Kyle H Vining, Jacob C Scherba, Alaina M Bever, Morgan R Alexander, Adam D Celiz, David J Mooney
Dental disease annually affects billions of patients, and while regenerative dentistry aims to heal dental tissue after injury, existing polymeric restorative materials, or fillings, do not directly participate in the healing process in a bioinstructive manner. There is a need for restorative materials that can support native functions of dental pulp stem cells (DPSCs), which are capable of regenerating dentin. A polymer microarray formed from commercially available monomers to rapidly identify materials that support DPSC adhesion is used...
December 7, 2017: Advanced Materials
https://www.readbyqxmd.com/read/29214501/mouse-embryonic-fibroblast-mef-bmp4-conditioned-medium-enhanced-multipotency-of-human-dental-pulp-cells
#15
Lu Liu, Zhengjun Peng, Zhezhen Xu, Haoquan Huang, Xi Wei
Dental pulp cells (DPCs) are valuable cell source for dental regeneration, albeit their application is restricted by limited pluripotency due to current culture condition. Mouse embryonic fibroblasts (MEFs) are served as feeder layer to maintain undifferentiated state of iPSCs and ESCs with long-term in vitro culture. Bone morphogenetic protein 4 (BMP4) plays an important role in the regulation of undifferentiated state and lineage commitment of cells through modulation of microenvironment. However, so far little was known how micro environment affect the multipotency of dental derived cells...
December 6, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29197101/a-biocompatible-decellularised-pulp-scaffold-for-regenerative-endodontics
#16
M Matoug-Elwerfelli, M S Duggal, H Nazzal, F Esteves, E Raïf
AIM: To investigate the feasibility of decellularising the entire dental pulp using a mild treatment protocol to develop a decellularised biological extracellular matrix scaffold for use in regenerative endodontic procedures. METHODOLOGY: Decellularised human dental pulps were assessed using histological and immunohistochemical methods, scanning electron microscope and DNA quantification assay. Cytotoxicity assays to determine decellularised scaffold biocompatibility were also performed...
December 1, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/29188652/-clinical-applications-of-stem-cells-from-human-exfoliated-deciduous-teeth-in-stem-cell-therapy
#17
Li Xiaoxia, Fangteng Jiaozi, Yu Shi, Zhao Yuming, Ge Lihong
Stem cells from human exfoliated deciduous teeth (SHED) are one category of dental stem cells. They belong to ectodermal mesenchymal stem cells. As an ideal stem cell source, SHED possess great potential in stem cell therapy. This review demonstrates the biological characteristics and advantages of SHED in stem cell therapy and discusses its multiple functions in tissue regeneration and repair, including multiple differentiation potentiality, cell secretion of cytokines, and immunomodulatory ability. Furthermore, this article introduces the main findings regarding the potential clinical applications of SHED to a variety of diseases...
October 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29180865/fabrication-of-large-pore-mesoporous-ca-si-based-bioceramics-for-bone-regeneration
#18
Deliang Zeng, Xingdi Zhang, Xiao Wang, Lingyan Cao, Ao Zheng, Jiahui Du, Yongsheng Li, Qingfeng Huang, Xinquan Jiang
Our previous study revealed that mesoporous Ca-Si-based materials exhibited excellent osteoconduction because dissolved ions could form a layer of hydroxycarbonate apatite on the surface of the materials. However, the biological mechanisms underlying bone regeneration were largely unknown. The main aim of this study was to evaluate the osteogenic ability of large-pore mesoporous Ca-Si-based bioceramics (LPMSCs) by alkaline phosphatase assay, real-time PCR analysis, von Kossa, and alizarin red assay. Compared with large-pore mesoporous silica (LPMS), LPMSCs had a better effect on the osteogenic differentiation of dental pulp cells...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29179973/mechanism-of-bioactive-molecular-extraction-from-mineralized-dentin-by-calcium-hydroxide-and-tricalcium-silicate-cement
#19
Xue-Qing Huang, John Camba, Li-Sha Gu, Brian E Bergeron, Domenico Ricucci, David H Pashley, Franklin R Tay, Li-Na Niu
OBJECTIVES: The objective of the present study was to elucidate the mechanism of bioactive molecule extraction from mineralized dentin by calcium hydroxide (Ca(OH)2) and tricalcium silicate cements (TSC). METHODS AND RESULTS: Transmission electron microscopy was used to provide evidence for collagen degradation in dentin surfaces covered with Ca(OH)2 or a set, hydrated TSC for 1-3 months. A one micron thick collagen degradation zone was observed on the dentin surface...
November 24, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29170828/a-novel-trifunctional-family-gh10-enzyme-from-acidothermus-cellulolyticus-11b-exhibiting-endo-xylanase-arabinofuranosidase-and-acetyl-xylan-esterase-activities
#20
Saher Shahid, Razia Tajwar, Muhammad Waheed Akhtar
A novel, family GH10 enzyme, Xyn10B from Acidothermus cellulolyticus 11B was cloned and expressed in Escherichia coli. This enzyme was purified to homogeneity by binding to regenerated amorphous cellulose. It had higher binding on Avicel as compared to insoluble xylan due to the presence of cellulose-binding domains, CBM3 and CBM2. This enzyme was optimally active at 70 °C and pH 6.0. It was stable up to 70 °C while the CD spectroscopy analysis showed thermal unfolding at 80 °C. Xyn10B was found to be a trifunctional enzyme having endo-xylanase, arabinofuranosidase and acetyl xylan esterase activities...
November 23, 2017: Extremophiles: Life Under Extreme Conditions
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