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Pulp regeneration

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https://www.readbyqxmd.com/read/28796952/implanted-dental-pulp-cells-fail-to-induce-regeneration-in-partial-pulpotomies
#1
F Mangione, M EzEldeen, C Bardet, J Lesieur, M Bonneau, F Decup, B Salmon, R Jacobs, C Chaussain, S Opsahl-Vital
Cell-based partial pulp regeneration is one of the promising approaches to obtain newly formed functional dentin-pulp complex. It relies on the preservation of the healthy tissue while regenerating the damaged pulp. The aim of this study was to investigate whether this regenerative process could be achieved by implanting porcine dental pulp cells (pDPCs) in pulp defects in the minipig. By split-mouth model, self-assembling injectable nanopeptide hydrogel, with and without pDPCs, was implanted after cameral pulpotomy in premolars and molars...
August 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28782906/differentiation-of-human-dental-pulp-stem-cells-into-neuronal-by-resveratrol
#2
Ya-Wei Geng, Zhen Zhang, Ming-Yue Liu, Wei-Ping Hu
Dental pulp stem cells (DPSCs) have been proposed as a promising source of stem cells in nerve regeneration due to their close embryonic origin and ease of harvest. Resveratrol (RSV) is a natural polyphenolic and possesses many biological functions such as anti-inflammatory activity and protection against atherosclerosis and neuroprotective activities. There is increasing evidence showing that RSV plays a pivotal role in neuron protection and neuronal differentiation. In this study, we isolated DPSCs from impacted third molars and investigated whether RSV induces neuronal differentiation of DPSCs...
August 7, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28781092/properties-of-dental-pulp-derived-mesenchymal-stem-cells-and-the-effects-of-culture-conditions
#3
Nobuyuki Kawashima, Sonoko Noda, Mioko Yamamoto, Takashi Okiji
Dental pulp mesenchymal stem cells (DPMSCs) highly express mesenchymal stem cell markers and possess the potential to differentiate into neural cells, osteoblasts, adipocytes, and chondrocytes. Thus, DPMSCs are considered suitable for tissue regeneration. The colony isolation method has commonly been used to collect relatively large amounts of heterogeneous DPMSCs. Homogenous DPMSCs can be isolated by fluorescence-activated cell sorting using antibodies against mesenchymal stem cell markers, although this method yields a limited number of cells...
August 3, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28781091/dental-pulp-stem-cells-their-potential-in-reinnervation-and-angiogenesis-by-using-scaffolds
#4
Ivo Lambrichts, Ronald B Driesen, Yörg Dillen, Pascal Gervois, Jessica Ratajczak, Tim Vangansewinkel, Esther Wolfs, Annelies Bronckaers, Petra Hilkens
Dental pulp is a highly vascularized and innervated tissue containing a heterogeneous stem cell population with multilineage differentiation potential. Current endodontic treatments focus on the preservation of the pulp tissue and the regeneration of dental pulp after pathological insults. Human dental pulp stem cells (hDPSCs) are currently investigated as stem cell-based therapy for pulp regeneration and for peripheral nerve injury in which neurons and Schwann cells display limited regenerative capacity. We have developed a neuronal differentiation protocol for hDPSCs that requires neurosphere formation before neuronal maturation...
August 3, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28779848/tgf-%C3%AE-1-stimulates-cyclooxygenase-2-expression-and-pge2-production-of-human-dental-pulp-cells-role-of-alk5-smad2-and-mek-erk-signal-transduction-pathways
#5
Po-Shuen Lin, Ru-Hsiu Cheng, Mei-Chi Chang, Jang-Jaer Lee, Hsiao-Hua Chang, Wei-Ling Huang, Sin-Yuet Yeung, Ya-Ching Chang, Jiiang-Huei Jeng
BACKGROUND/PURPOSES: TGF-β1 is an important growth factor that may influence the odontoblast differentiation and matrix deposition in the reactionary/reparative dentinogenesis to dental caries or other tooth injuries. TGF-β1 exerts its effects through various signaling pathways, such as Smads and MAPKs. Cyclooxygenase-2 (COX-2) is a membrane-associated enzyme that produces prostaglandin E2 (PGE2) at sites of pulpal injury and inflammation, which leads to tissue swelling, redness and pain...
August 2, 2017: Journal of the Formosan Medical Association, Taiwan Yi Zhi
https://www.readbyqxmd.com/read/28773189/evaluation-of-3d-printed-polycaprolactone-scaffolds-coated-with-freeze-dried-platelet-rich-plasma-for-bone-regeneration
#6
Junda Li, Meilin Chen, Xiaoying Wei, Yishan Hao, Jinming Wang
Three-dimensional printing is one of the most promising techniques for the manufacturing of scaffolds for bone tissue engineering. However, a pure scaffold is limited by its biological properties. Platelet-rich plasma (PRP) has been shown to have the potential to improve the osteogenic effect. In this study, we improved the biological properties of scaffolds by coating 3D-printed polycaprolactone (PCL) scaffolds with freeze-dried and traditionally prepared PRP, and we evaluated these scaffolds through in vitro and in vivo experiments...
July 19, 2017: Materials
https://www.readbyqxmd.com/read/28765825/comparison-of-the-effect-of-prp-prf-and-induced-bleeding-in-the-revascularization-of-teeth-with-necrotic-pulp-and-open-apex-a-triple-blind-randomized-clinical-trial
#7
Vasundara Yayathi Shivashankar, Dexton Antony Johns, Ramesh Kumar Maroli, Mahalaxmi Sekar, Rathinavel Chandrasekaran, Shanmugavel Karthikeyan, Senthil Kumar Renganathan
INTRODUCTION: Treatment of a tooth with necrotic pulp and open apex is a special challenge to the clinicians. Apexification with calcium hydroxide and MTA barrier technique fails to induce continued root maturation which makes the tooth susceptible to root fracture. Hence, an ideal outcome for such a tooth should be regeneration of pulp like tissue into the root canal capable of continuing normal root maturation. AIM: This study aims to compare the effect of Platelet Rich Fibrin (PRF), induced bleeding technique and Platelet Rich Plasma (PRP) in the revascularization of tooth with necrotic pulp and open apex...
June 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28764928/tissue-engineering-dentin-pulp-complex-regeneration-approaches-a-review
#8
REVIEW
Batool Hashemi-Beni, Maryam Khoroushi, Mohammad Reza Foroughi, Saeed Karbasi, Abbas Ali Khademi
Dental pulp is a highly specialized tissue that preserves teeth. It is important to maintain the capabilities of dental pulp before a pulpectomy by creating a local restoration of the dentin-pulp complex from residual dental pulp. The articles identified were selected by two reviewers based on entry and exit criteria. All relevant articles indexed in PubMed, Springer, Science Direct, and Scopus with no limitations from 1961 to 2016 were searched. Factors investigated in the selected articles included the following key words: Dentin-Pulp Complex, Regeneration, Tissue Engineering, Scaffold, Stem Cell, and Growth Factors...
July 25, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28764802/mesenchymal-stem-cells-derived-from-inflamed-dental-pulpal-and-gingival-tissue-a-potential-application-for-bone-formation
#9
Laura Tomasello, Rodolfo Mauceri, Antonina Coppola, Maria Pitrone, Giuseppe Pizzo, Giuseppina Campisi, Giuseppe Pizzolanti, Carla Giordano
BACKGROUND: Chronic periodontal disease is an infectious disease consisting of prolonged inflammation of the supporting tooth tissue and resulting in bone loss. Guided bone regeneration procedures have become common and safe treatments in dentistry, and in this context dental stem cells would represent the ideal solution as autologous cells. In this study, we verified the ability of dental pulp mesenchymal stem cells (DPSCs) and gingival mesenchymal stem cells (GMSCs) harvested from periodontally affected teeth to produce new mineralized bone tissue in vitro, and compared this to cells from healthy teeth...
August 1, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28751918/cellular-responses-in-human-dental-pulp-stem-cells-treated-with-three-endodontic-materials
#10
Alejandro Victoria-Escandell, José Santiago Ibañez-Cabellos, Sergio Bañuls-Sánchez de Cutanda, Ester Berenguer-Pascual, Jesús Beltrán-García, Eva García-López, Federico V Pallardó, José Luis García-Giménez, Antonio Pallarés-Sabater, Ignacio Zarzosa-López, Manuel Monterde
Human dental pulp stem cells (HDPSCs) are of special relevance in future regenerative dental therapies. Characterizing cytotoxicity and genotoxicity produced by endodontic materials is required to evaluate the potential for regeneration of injured tissues in future strategies combining regenerative and root canal therapies. This study explores the cytotoxicity and genotoxicity mediated by oxidative stress of three endodontic materials that are widely used on HDPSCs: a mineral trioxide aggregate (MTA-Angelus white), an epoxy resin sealant (AH-Plus cement), and an MTA-based cement sealer (MTA-Fillapex)...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28750661/human-dental-pulp-pluripotent-like-stem-cells-promote-wound-healing-and-muscle-regeneration
#11
Ester Martínez-Sarrà, Sheyla Montori, Carlos Gil-Recio, Raquel Núñez-Toldrà, Domiziana Costamagna, Alessio Rotini, Maher Atari, Aernout Luttun, Maurilio Sampaolesi
BACKGROUND: Dental pulp represents an easily accessible autologous source of adult stem cells. A subset of these cells, named dental pulp pluripotent-like stem cells (DPPSC), shows high plasticity and can undergo multiple population doublings, making DPPSC an appealing tool for tissue repair or maintenance. METHODS: DPPSC were harvested from the dental pulp of third molars extracted from young patients. Growth factors released by DPPSC were analysed using antibody arrays...
July 27, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28748053/allogeneic-stem-cells-derived-from-human-exfoliated-deciduous-teeth-shed-for-the-management-of-periapical-lesions-in-permanent-teeth-two-case-reports-of-a-novel-biologic-alternative-treatment
#12
Madu Ghana Shyam Prasad, Juvva Ramakrishna, Duvvi Naveen Babu
Stem cells are the pluripotent cells that have the capacity to differentiate into other specialized cells. Recently, many experiments have been conducted to study the potentiality of stem cells in the tissue regeneration. We report two cases treated utilizing stem cells from human exfoliated deciduous teeth (SHED) in the management of periapical lesions in permanent teeth. Two normal human deciduous teeth from children, 7‒8 years of age, were collected to isolate stem cells. Two patients, one with periapical pathology alone and the other with periapical lesion along with an open apex in young permanent teeth, were selected for the study...
2017: Journal of Dental Research, Dental Clinics, Dental Prospects
https://www.readbyqxmd.com/read/28741725/cell-derived-micro-environment-helps-dental-pulp-stem-cells-promote-dental-pulp-regeneration
#13
Xuexin Zhang, Hui Li, Jingjing Sun, Xiangyou Luo, Hefeng Yang, Li Xie, Bo Yang, Weihua Guo, Weidong Tian
OBJECTIVES: The function of the dental pulp is closely connected to the extracellular matrix (ECM) structure, and ECM has received significant attention due to its biological functions for regulating cells. As such, the interaction between the ECM niche and cells is worth exploring for potential clinical uses. MATERIALS AND METHODS: In this study, dental pulp stem cell (DPSC)-derived ECM (DPM) was prepared through cell culture and decellularization to function as the cell niche, and changes in DPSC behaviour and histological analysis of dental pulp tissue regeneration were evaluated following the DPM culture...
July 25, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28727999/drug-ion-co-delivery-multi-functional-nanocarrier-to-regenerate-infected-tissue-defect
#14
Jung-Hwan Lee, Ahmed El-Fiqi, Nandin Mandakhbayar, Hae-Hyoung Lee, Hae-Won Kim
Regeneration of infected tissues is a globally challenging issue in medicine and dentistry. Common clinical therapies involving a complete removal of infected areas together with a treatment of antimicrobial drugs are often suboptimal. Biomaterials with anti-bacterial and pro-regenerative potential can offer a solution to this. Here we design a novel nanocarrier based on a mesoporous silicate-calcium glass by doping with Ag ions and simultaneously loading antimicrobial drugs onto mesopores. The nanocarriers could controllably release multiple ions (silver, calcium, and silicate) and drugs (tetracycline or chlorohexidine) to levels therapeutically relevant, and effectively internalize to human dental stem cells (∼90%) with excellent viability, ultimately stimulating odontogenic differentiation...
October 2017: Biomaterials
https://www.readbyqxmd.com/read/28713908/comparison-of-gene-expression-profiles-between-dental-pulp-and-periodontal-ligament-tissues-in-humans
#15
Ai-Xiu Gong, Jing-Han Zhang, Jing Li, Jun Wu, Lin Wang, Deng-Shun Miao
There are anatomical and functional differences between human dental pulp (DP) and periodontal ligament (PDL). However, the molecular biological differences and function of these tissues are poorly understood. In the present study, we employed a cDNA microarray array to screen for differentially expressed genes (DEGs) between human DP and PDL tissues, and used the online software WebGestalt to perform the functional analysis of the DEGs. In addition, the STRING database and KEGG pathway analysis were applied for interaction network and pathway analysis of the DEGs...
September 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28713017/intracellular-co-delivery-of-sr-ion-and-phenamil-drug-through-mesoporous-bioglass-nanocarriers-synergizes-bmp-signaling-and-tissue-mineralization
#16
Jung-Hwan Lee, Nandin Mandakhbayar, Ahmed El-Fiqi, Haw-Won Kim
Inducing differentiation and maturation of resident multipotent stem cells (MSCs) is an important strategy to regenerate hard tissues in mal-calcification conditions. Here we explore a co-delivery approach of therapeutic molecules comprised of ion and drug through a mesoporous bioglass nanoparticle (MBN) for this purpose. Recently, MBN has offered unique potential as a nanocarrier for hard tissues, in terms of high mesoporosity, bone bioactivity (and possibly degradability), tunable delivery of biomolecules, and ionic modification...
July 13, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28692829/effects-of-melatonin-on-the-proliferation-and-differentiation-of-human-dental-pulp-cells
#17
Qin Liu, Wenguo Fan, Yifan He, Fuping Zhang, Xiaoyan Guan, Qianyi Deng, Xianjun Lu, Hongwen He, Fang Huang
OBJECTIVE: Effects of melatonin on the proliferation and differentiation of human dental pulp cells (hDPCs) remain unclear. The purpose of this study was to investigate the effect of melatonin on the proliferation and differentiation of the hDPCs. DESIGN: Primary hDPCs were obtained from the third molar of volunteer aged from 18 to 25. CCK8 assay evaluated the effect of melatonin upon cell proliferation at day 1, 2, 3, 4, 5. After 7days' osteogenic induction with melatonin or vehicle, alkaline phosphatase (ALP) activity was measured with a commercial kit...
June 30, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28671698/sustainable-sources-need-reliable-standards
#18
Maxim V Galkin, Davide Di Francesco, Ulrica Edlund, Joseph S M Samec
This review discusses the challenges within the research area of modern biomass fractionation and valorization. The current pulping industry focuses on pulp production and the resulting cellulose fiber. Hemicellulose and lignin are handled as low value streams for process heat and the regeneration of process chemicals. The paper and pulp industry have therefore developed analytical techniques to evaluate the cellulose fiber, while the other fractions are given a low priority. In a strive to also use the hemicellulose and lignin fractions of lignocellulosic biomass, moving towards a biorefining concept, there are severe shortcomings with the current pulping techniques and also in the analysis of the biomass...
July 3, 2017: Faraday Discussions
https://www.readbyqxmd.com/read/28667922/combination-of-hydroxyapatite-platelet-rich-fibrin-and-amnion-membrane-as-a-novel-therapeutic-option-in-regenerative-periapical-endodontic-surgery-case-series
#19
Uday Kiran Uppada, Butchibabu Kalakonda, Pradeep Koppolu, Narendra Varma, Kiran Palakurthy, Venkatesh Manchikanti, Shilpa Prasad, Shereen Samar, Lingam Amara Swapna
INTRODUCTION: Periapical surgery is the last resort in the arsenal of an endodontist to effectively deal with periapical lesions that result from necrosis of the pulp. Bone grafts, growth factors and membranes form an array of regenerative materials that influence the healing outcome of periapical surgery. PRESENTATION OF CASE: The main purpose of the two cases reported here was to assess the potential benefits of a combination of bone graft, platelet-rich fibrin (PRF) and amnion membrane in terms of reduced post-operative discomfort, radiographic evidence of accelerated periapical bone healing and present a novel therapeutic option in the management of large periapical lesions...
2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/28662879/use-of-platelet-rich-plasma-in-endodontic-procedures-in-adults-regeneration-or-repair-a-report-of-3-cases-with-5%C3%A2-years-of-follow-up
#20
José Francisco Gaviño Orduña, Javier Caviedes-Bucheli, María C Manzanares Céspedes, Esther Berástegui Jimeno, Benjamín Martín Biedma, Juan José Segura-Egea, José López-López
INTRODUCTION: Regenerative endodontic procedures (REP) are a promising alternative for necrotic immature teeth in adolescents and children, but very little evidence is available on this alternative in long-lasting necrotic teeth with open apices in adults. REPs are designed to replace damaged structures of the pulp-dentin complex, but no regeneration has been obtained in any of the cases described in necrotic immature permanent teeth with apical periodontitis with histologic results. The results are limited to periapical tissue repair with increasing root length, thickening of the root walls, and apical closure in young patients...
August 2017: Journal of Endodontics
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