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https://www.readbyqxmd.com/read/28636235/stem-cell-based-tooth-and-periodontal-regeneration
#1
Lei Hu, Yi Liu, Songlin Wang
Currently regeneration of tooth and periodontal damage still remains great challenge. Stem cell-based tissue engineering raised novel therapeutic strategies for tooth and periodontal repair. Stem cells for tooth and periodontal regeneration include dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs), stem cells from the dental apical papilla (SCAPs), and stem cells from human exfoliated deciduous teeth (SHEDs), dental follicle stem cells (DFSCs), dental epithelial stem cells (DESCs), bone marrow mesenchymal stem cells (BMMSCs), adipose-derived stem cells (ADSCs), embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs)...
June 21, 2017: Oral Diseases
https://www.readbyqxmd.com/read/28631418/the-effects-of-biodentine-polycaprolactone-3d-scaffold-with-odontogenesis-properties-on-human-dental-pulp-cells
#2
C-C Ho, H-Y Fang, B Wang, T-H Huang, M-Y Shie
AIM: To determine the feasibility of using three-dimensional printed Biodentine/polycaprolactone composite scaffolds for orthopaedic and dental applications. The physicochemical properties and the odontogenic differentiation of human dental pulp cells (hDPCs) were investigated. METHODOLOGY: Biodentine was well-suspended in ethanol and dropped slowly into molten polycaprolactone with vigorous stirring. The Biodentine/polycaprolactone composite scaffolds were then fabricated into controlled macropore sizes and structures using an extrusion-based three-dimensional (3D) printer...
June 20, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28616151/therapeutic-potential-of-dental-stem-cells
#3
REVIEW
Elna Paul Chalisserry, Seung Yun Nam, Sang Hyug Park, Sukumaran Anil
Stem cell biology has become an important field in regenerative medicine and tissue engineering therapy since the discovery and characterization of mesenchymal stem cells. Stem cell populations have also been isolated from human dental tissues, including dental pulp stem cells, stem cells from human exfoliated deciduous teeth, stem cells from apical papilla, dental follicle progenitor cells, and periodontal ligament stem cells. Dental stem cells are relatively easily obtainable and exhibit high plasticity and multipotential capabilities...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28611689/monitoring-notch-signaling-associated-activation-of-stem-cell-niches-within-injured-dental-pulp
#4
Thimios A Mitsiadis, Javier Catón, Pierfrancesco Pagella, Giovanna Orsini, Lucia Jimenez-Rojo
Dental pulp stem/progenitor cells guarantee tooth homeostasis, repair and regeneration throughout life. The decision between renewal and differentiation of these cells is influenced by physical and molecular interactions with stromal cells and extracellular matrix molecules forming the specialized microenvironment of dental pulp stem cell niches. Here we study the activation of putative pulp niches after tooth injury through the upregulation of Notch signaling pathway. Notch1, Notch2, and Notch3 molecules were used as markers of dental pulp stem/progenitor cells...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28608628/the-role-of-microrna-143-5p-in-the-differentiation-of-dental-pulp-stem-cells-into-odontoblasts-by-targeting-runx2-via-the-opg-rankl-signaling-pathway
#5
Fu-Liang Zhan, Xin-Yang Liu, Xing-Bo Wang
This study aims to elucidate the mechanisms by which microRNA-143-5p (miR-143-5p) targets runt-related transcription factor 2 (Runx2) in the differentiation of dental pulp stem cells (DPSCs) into odontoblasts, through regulating the osteoprotegerin receptor activator of the nuclear factor-κB ligand (OPG/RANKL) signaling pathway. Following transfection, DPSCs were divided into blank, control, miR-143-5p mimics, miR-143-5p inhibitors, miR-143-5p inhibitors + siRunx2 and siRunx2 groups. Alkaline phosphatase (ALP) activity and mineralized nodules were detected using ALP kit and alizarin red staining...
June 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28607361/a-novel-strategy-to-engineer-pre-vascularized-full-length-dental-pulp-like-tissue-constructs
#6
Avathamsa Athirasala, Fernanda Lins, Anthony Tahayeri, Monica Hinds, Anthony J Smith, Christine Sedgley, Jack Ferracane, Luiz E Bertassoni
The requirement for immediate vascularization of engineered dental pulp poses a major hurdle towards successful implementation of pulp regeneration as an effective therapeutic strategy for root canal therapy, especially in adult teeth. Here, we demonstrate a novel strategy to engineer pre-vascularized, cell-laden hydrogel pulp-like tissue constructs in full-length root canals for dental pulp regeneration. We utilized gelatin methacryloyl (GelMA) hydrogels with tunable physical and mechanical properties to determine the microenvironmental conditions (microstructure, degradation, swelling and elastic modulus) that enhanced viability, spreading and proliferation of encapsulated odontoblast-like cells (OD21), and the formation of endothelial monolayers by endothelial colony forming cells (ECFCs)...
June 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28606672/three-dimensional-sphere-forming-cells-are-unique-multipotent-cell-population-in-dental-pulp-cells
#7
Sung Hee Lee, Andrew Inaba, Neha Mohindroo, Deepika Ganesh, Charlotte Ellen Martin, Nadia Chugal, Reuben H Kim, Mo K Kang, No-Hee Park, Ki-Hyuk Shin
INTRODUCTION: Mesenchymal stem cells (MSCs) are typically cultured as adherent monolayer using a conventional tissue culture technique. However, this technique incompletely reproduces an in vivo microenvironment of stem cells and results in the loss of stemness properties. Three-dimensional (3D) sphere culture is one of the most widely used 3D culture techniques that have been developed to recapitulate the in vivo microenvironment. However, the stemness and multilineage differentiation capacity of spheres derived from dental pulp stem cells (DPSCs) have not been well investigated...
June 9, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28595566/a-systemic-review-of-the-sources-of-adult-mesenchymal-stem-cells-and-their-suitability-in-musculoskeletal-applications
#8
Rhiannon Nancarrow-Lei, Pouya Mafi, Reza Mafi, Wasim Khan
Background: Adult mesenchymal stem cells (MSCs) were first isolated from bone marrow by Friedenstein in 1976. These cells were clonogenic, non-haematopoietic, and able to replicate extensively in vitro. The fields of regenerative medicine and tissue engineering have grown dramatically since their inception. In the decades since, MSCs have been identified from mesoderm-, endoderm- and ectoderm-derived tissues. In light of our ageing population, the need for effective cell-based therapies for tissue repair and regeneration is ever-expanding...
June 8, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28581212/glial-cell-derived-neurotrophic-factor-promotes-dental-pulp-stem-cell-migration
#9
Nan Xiao, Wei Ye Yu, Dawei Liu
Preserving the vitality of the teeth is critical in maintaining the function and esthetics of teeth during dental treatment. Dental pulp stem cells (DPSCs) are mesenchymal cells that are demonstrated to possess stem cell properties, such as self-renewal, proliferation and pluripotency. DPSCs can be obtained through non-invasive procedure from the dental pulp and become potential resources for tissue regeneration. Neurotrophic factors are known to promote survival and growth of neurons. In the present study, we examined the expression of the glial cell derived neurotrophic factor (GDNF) family ligands and receptors, and characterized the intracellular localization of them in DPSCs...
June 5, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28580523/effects-of-combined-transplantation-of-multipotent-mesenchymal-stromal-and-hemopoietic-stem-cells-on-regeneration-of-the-hemopoietic-tissue
#10
I Yu Maklakova, D Yu Grebnev
The effect of allogenic combined transplantation of placental multipotent mesenchymal stromal and hemopoietic stem cells on regeneration of the myeloid tissue and spleen after acute blood loss was studied in laboratory mice. Combined transplantation of these cells did not change the content of cytogenetically modified cells in the bone marrow under normal conditions, but reduced their levels after acute blood loss. Combined transplantation of multipotent mesenchymal stromal and hemopoietic stem cells promoted activation of erythropoiesis and granulocytopoiesis...
June 5, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28577961/histologic-evaluation-of-regenerative-endodontic-procedures-with-the-use-of-chitosan-scaffolds-in-immature-dog-teeth-with-apical-periodontitis
#11
Paulo J Palma, João C Ramos, João B Martins, Anibal Diogenes, Maria H Figueiredo, Paula Ferreira, Carlos Viegas, João M Santos
INTRODUCTION: The aim of this study was to evaluate histologically the newly formed tissues after regenerative endodontic procedures (REPs) in dogs using either a blood clot (BC) or 2 different formulations of a chitosan hydrogel as scaffolds. METHODS: Apical periodontitis was induced by inoculating immature teeth with oral plaque in 4 beagle dogs. Teeth (n = 96) were divided into 2 control (n = 20) and 4 test groups (n = 76) according to the treatment: apexification and REPs with BC, sodium hyaluronate:chitosan (HA:CS) scaffolds, or pectin:chitosan (P:CS) scaffolds...
May 31, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28547570/the-effect-of-iloprost-on-cell-proliferation-and-angiogenesis-related-gene-expression-in-human-periodontal-ligament-cells
#12
Thanomsuk Jearanaiphaisarn, Teeranuch Sanharati, Prasit Pavasant, Chalida Nakalekha Limjeerajarus
Periodontal ligament is considered a rich source of mesenchymal stem cells for pulp regeneration. This study investigated the effect of iloprost, a prostacyclin analog, on cell proliferation and the expression of angiogenesis-related genes in human periodontal ligament cells (hPDLs) in vitro. The hPDLs were treated with 10(-9)-10(-6) M iloprost for 1, 6 and 24 h. Cell proliferation was determined by an MTT assay. Vascular endothelial growth factor (VEGF), alpha-1 type I collagen (COL1), and basic fibroblast growth factor (bFGF) mRNA expression were determined by semi-quantitative and qPCR...
May 25, 2017: Odontology
https://www.readbyqxmd.com/read/28545523/l-mimosine-and-hypoxia-can-increase-angiogenin-production-in-dental-pulp-derived-cells
#13
Klara Janjić, Michael Edelmayer, Andreas Moritz, Hermann Agis
BACKGROUND: Angiogenin is a key molecule in the healing process which has been successfully applied in the field of regenerative medicine. The role of angiogenin in dental pulp regeneration is unclear. Here we aimed to reveal the impact of the hypoxia mimetic agent L-mimosine (L-MIM) and hypoxia on angiogenin in the dental pulp. METHODS: Human dental pulp-derived cells (DPC) were cultured in monolayer and spheroid cultures and treated with L-MIM or hypoxia. In addition, tooth slice organ cultures were applied to mimic the pulp-dentin complex...
May 25, 2017: BMC Oral Health
https://www.readbyqxmd.com/read/28544201/influence-of-highly-porous-electrospun-plga-pcl-nha-fibrous-scaffolds-on-the-differentiation-of-tooth-bud-cells-in-vitro
#14
Xinjie Cai, Sofie Ten Hoopen, Weibo Zhang, Charles Yi, Wanxun Yang, Fang Yang, John A Jansen, X Frank Walboomers, Pamela C Yelick
In this study, we investigated the use of three-dimensional electrospun poly(lactic-co-glycolic acid)/poly(ε-caprolactone) (PLGA/PCL) scaffolds seeded and cultured with postnatal dental cells, for improved dental tissue regeneration. Wet-electrospinning combined with ultrasonic treatment was studied as a method to enhance scaffold porosity and to promote cell-cell interactions. We also investigated whether nano-hydroxyapatite (nHA) incorporation could enhance dental cell differentiation. All scaffolds were seeded with human tooth pulp-derived dental mesenchymal (hDM) cells, or a combination of hDM and pig dental epithelial (pDE) cells, cultured for up to 28 days...
May 24, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28542487/characterization-of-micrornas-expression-profiles-in-human-dental-derived-pluripotent-stem-cells
#15
Xiaobing Tan, Qingyuan Dai
Induced pluripotent stem cells (iPSCs) technology provides a powerful means to generate and regenerate unlimited pluripotent stem cells directly from body tissue cells. Stem cells from apical papilla (SCAP) and Dental pulp stem cells (DPSCs) are present in 'cell-rich zones' within the dental pulp region, which are capable of regenerating pulp and dentin tissues in vivo. In this study, we investigated the difference of miRNAs expression in SCAPs and DPSCs before and after the reprogramming. Using miRNA microarray, 134 and 265 differentially expressed miRNAs in DPSCs- and SCAP-iPSCs were up-regulated compared to these before reprogramming...
2017: PloS One
https://www.readbyqxmd.com/read/28539292/-gelatin-alginate-hydrogel-scaffolds-prepared-by-3d-bioprinting-promotes-cell-adhesion-and-proliferation-of-human-dental-pulp-cells-in-vitro
#16
Hai-Yue Yu, Dan-Dan Ma, Bu-Ling Wu
OBJECTIVE: To evaluate the cytotoxicity of gelatin/alginate hydrogel scaffolds prepared by 3D bioprinting in human dental pulp cells (HDPCs) and compare the cell adhesion and proliferation of the cells seeded in the biomaterial using two different methods. METHODS: HDPCs isolated by tissue block culture and enzyme digestion were cultured and passaged. Gelatin/alginate hydrogel scaffolds were printed using a bioplotter, and the cytotoxicity of the aqueous extracts of the scaffold material was tested in the third passage of HDPCs using cell counting kit-8...
May 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28531913/methods-of-isolation-and-characterization-of-stem-cells-from-different-regions-of-oral-cavity-using-markers-a-systematic-review
#17
REVIEW
Kavarthapu Avinash, Sankari Malaippan, Jayakumar Nadathur Dooraiswamy
Background: Periodontitis is a destructive inflammatory disorder of the periodontium caused by the destruction of periodontal tissues namely the PDL, cementum, alveolar bone, and gingiva. Once these tissues are lost, the foremost goal of periodontal therapy is to regenerate the diseased tissues if possible to their original form, architecture, and function. Various regenerative procedures were employed and still a gap was found in achieving the goal. As stem cells are characterized by their ability to self-renew and differentiate to produce specialized cells, there could be a possibility of using them for regenerative therapy...
May 30, 2017: International Journal of Stem Cells
https://www.readbyqxmd.com/read/28530133/efficacy-of-a-self-assembling-peptide-hydrogel-spg-178-gel-for-bone-regeneration-and-three-dimensional-osteogenic-induction-of-dental-pulp-stem-cells
#18
Jun Tsukamoto, Keiko Naruse, Yusuke Nagai, Shuhei Kan, Nobuhisa Nakamura, Masaki Hata, Maiko Omi, Tatsuhide Hayashi, Tatsushi Kawai, Tatsuaki Matsubara
The aim of this study was to assess the efficacy of a self-assembling peptide hydrogel as a scaffold for bone regeneration. We used a neutral and injectable self-assembling peptide hydrogel, SPG-178-Gel. Bone defects (5 mm in diameter) in rat calvarial bones were filled with a mixture of alpha-modified Eagle's medium and the peptide hydrogel. Three weeks after surgery, soft X-ray and micro-computed tomography (micro-CT) images of the gel-treated bones showed new bone formations in the periphery and in central areas of the defects...
May 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28527850/complement-activation-by-pulp-capping-materials-plays-a-significant-role-in-both-inflammatory-and-pulp-stem-cells-recruitment
#19
Thomas Giraud, Pierre Rufas, Fanny Chmilewsky, Charlotte Rombouts, Jacques Dejou, Charlotte Jeanneau, Imad About
INTRODUCTION: The role of complement, especially through the C5a fragment, is well-known for the initiation of inflammation. Its involvement in regeneration has been shown more recently by the recruitment of mesenchymal stem cells. C5a can be produced locally by the pulp fibroblasts in response to injury or infection. This work aims to investigate the effect of different pulp capping biomaterials on complement activation and its possible influence on inflammatory and pulp stem cell recruitment...
May 17, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28527838/pulp-inflammation-diagnosis-from-clinical-to-inflammatory-mediators-a-systematic-review
#20
REVIEW
Marjorie Zanini, Elisabeth Meyer, Stéphane Simon
INTRODUCTION: Similar to other tissues, the dental pulp mounts an inflammatory reaction as a way to eliminate pathogens and stimulate repair. Pulp inflammation is prerequisite for dentin pulp complex repair and regeneration; otherwise, chronic disease or pulp necrosis occurs. Evaluation of pulp inflammation severity is necessary to predict the clinical success of maintaining pulp vitality. Clinical limitations to evaluating in situ inflammatory status are well-described. A molecular approach that aids clinical distinction between reversible and irreversible pulpitis could improve the success rate of vital pulp therapy...
May 17, 2017: Journal of Endodontics
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