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Pulp regeneration

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https://www.readbyqxmd.com/read/28079309/bioactive-injectable-aggregates-with-nanofibrous-microspheres-and-human-dental-pulp-stem-cells-a-translational-strategy-in-dental-endodontics
#1
I Garzón, M A Martin-Piedra, V Carriel, M Alaminos, X Liu, R D Souza
Regeneration of the pulp-dentin complex with stem cells is a potential alternative to conventional root canal treatments. Human dental pulp stem cells (hDPSCs) have been extensively studied because of their ability to proliferate and differentiate into mineralized dental and nondental tissues. In this work we combined hDPSCs with two types of injectable poly-L-lactic acid (PLLA) microspheres with a nanofibrous or smooth surface to form bioactive injectable aggregates, and examined their ability to promote pulp regeneration in the root canal in an in vivo model...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28070691/biological-characteristic-effects-of-human-dental-pulp-stem-cells-on-poly-%C3%AE%C2%B5-caprolactone-biphasic-calcium-phosphate-fabricated-scaffolds-using-modified-melt-stretching-and-multilayer-deposition
#2
Natkrita Wongsupa, Thongchai Nuntanaranont, Suttatip Kamolmattayakul, Nuttawut Thuaksuban
Craniofacial bone defects such as alveolar cleft affect the esthetics and functions that need bone reconstruction. The advanced techniques of biomaterials combined with stem cells have been a challenging role for maxillofacial surgeons and scientists. PCL-coated biphasic calcium phosphate (PCL-BCP) scaffolds were created with the modified melt stretching and multilayer deposition (mMSMD) technique and merged with human dental pulp stem cells (hDPSCs) to fulfill the component of tissue engineering for bone substitution...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28052540/engineered-neural-tissue-with-schwann-cell-differentiated-human-dental-pulp-stem-cells-potential-for-peripheral-nerve-repair
#3
Kathleen Sanen, Wendy Martens, Melanie Georgiou, Marcel Ameloot, Ivo Lambrichts, James Phillips
Despite the spontaneous regenerative capacity of the peripheral nervous system, large gap peripheral nerve injuries (PNIs) require bridging strategies. The limitations and suboptimal results obtained with autografts or hollow nerve conduits in the clinic urge the need for alternative treatments. Recently, we have described promising neuroregenerative capacities of Schwann cells derived from differentiated human dental pulp stem cells (d-hDPSCs) in vitro. Here, we extended the in vitro assays to show the pro-angiogenic effects of d-hDPSCs, such as enhanced endothelial cell proliferation, migration and differentiation...
January 4, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28050518/revitalization-of-an-immature-permanent-mandibular-molar-with-a-necrotic-pulp-using-platelet-rich-fibrin-a-case-report
#4
Dayalan Subash, Krishnamma Shoba, Shibu Aman, Srinivasan Kumar Indu Bharkavi
Any insult to the pulp during its development causes cessation of dentin formation and root growth. Pulpal status and degree of root development are the decisive factors in the treatment approach. Various treatment options have been tried like surgery with root-end sealing, calcium hydroxide-apexification, placement of apical plug and regenerative endodontic procedures to induce apexogenesis. An ideal scenario for a necrosed tooth with immature root would be continued root development coupled with regeneration of pulp tissue...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28041686/evaluation-of-a-commercially-available-hyaluronic-acid-hydrogel-restylane-as-injectable-scaffold-for-dental-pulp-regeneration-an-in%C3%A2-vitro-evaluation
#5
Vanessa Chrepa, Obadah Austah, Anibal Diogenes
INTRODUCTION: Regenerative endodontic procedures (REPs) are viable alternatives for treating immature teeth, yet these procedures do not predictably lead to pulp-dentin regeneration. A true bioengineering approach for dental pulp regeneration requires the incorporation of a scaffold conducive with the regeneration of the pulp-dentin complex. Several materials have been proposed as scaffolds for REPs; nonetheless, the majority are not eligible for immediate clinical chairside use. Thus, the aim of this study was to evaluate Restylane, a Food and Drug Administration-approved hyaluronic acid-based gel, as possible scaffold for REPs...
December 29, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/28033065/effects-of-bioactive-compounds-on-odontogenic-differentiation-and-mineralization
#6
S Phung, C Lee, C Hong, M Song, J K Yi, R G Stevenson, M K Kang, K-H Shin, N-H Park, R H Kim
Direct pulp capping involves the placement of dental materials directly onto vital pulp tissues after deep caries removal to stimulate the regeneration of reparative dentin. This physical barrier will serve as a "biological seal" between these materials and the pulp tissue. Although numerous direct pulp capping materials are available, the use of small bioactive compounds that can potently stimulate and expedite reparative dentin formation is still underexplored. Here, the authors compared and evaluated the pro-osteogenic and pro-odontogenic effects of 4 small bioactive compounds- phenamil (Phen), purmorphamine (Pur), genistein (Gen), and metformin (Met)...
January 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28033061/c5l2-receptor-represses-brain-derived-neurotrophic-factor-secretion-in-lipoteichoic-acid-stimulated-pulp-fibroblasts
#7
F Chmilewsky, I About, S H Chung
The anaphylatoxin C5a constitutes a powerful fragment generated by complement system activation. Interestingly, this complement active fragment is also an important mediator of tissue regeneration. Recent findings suggest that C5a could be an initial signal orchestrating pulp nerve sprouting beneath carious injury, a critical step in dentin-pulp regeneration. Indeed, the expression and activation of the C5a active receptor (C5aR/CD88) by injured pulp fibroblasts controls the direction of neurite outgrowth toward carious injuries by modulating the secretion of brain-derived neurotrophic factor (BDNF) by pulp fibroblasts...
January 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28018144/deciphering-the-epigenetic-code-in-embryonic-and-dental-pulp-stem-cells
#8
REVIEW
Dashzeveg Bayarsaihan
A close cooperation between chromatin states, transcriptional modulation, and epigenetic modifications is required for establishing appropriate regulatory circuits underlying self-renewal and differentiation of adult and embryonic stem cells. A growing body of research has established that the epigenome topology provides a structural framework for engaging genes in the non-random chromosomal interactions to orchestrate complex processes such as cell-matrix interactions, cell adhesion and cell migration during lineage commitment...
December 2016: Yale Journal of Biology and Medicine
https://www.readbyqxmd.com/read/28005222/effects-of-nerve-growth-factor-and-basic-fibroblast-growth-factor-promote-human-dental-pulp-stem-cells-to-neural-differentiation
#9
Jinlong Zhang, Min Lian, Peipei Cao, Guofeng Bao, Guanhua Xu, Yuyu Sun, Lingling Wang, Jiajia Chen, Yi Wang, Guijuan Feng, Zhiming Cui
Dental pulp stem cells (DPSCs) were the most widely used seed cells in the field of neural regeneration and bone tissue engineering, due to their easily isolation, lack of ethical controversy, low immunogenicity and low rates of transplantation rejection. The purpose of this study was to investigate the role of basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) on neural differentiation of DPSCs in vitro. DPSCs were cultured in neural differentiation medium containing NGF and bFGF alone or combination for 7 days...
December 22, 2016: Neurochemical Research
https://www.readbyqxmd.com/read/28000851/three-dimensional-simulated-microgravity-culture-improves-the-proliferation-and-odontogenic-differentiation-of-dental-pulp-stem-cell-in-plga-scaffolds-implanted-in-mice
#10
Yanping Li, Lina He, Shuang Pan, Lin Zhang, Weiwei Zhang, Hong Yi, Yumei Niu
Tooth regeneration through stem cell-based therapy is a promising treatment for tooth decay and loss. Human dental pulp stem cells (hDPSCs) have been widely identified as the stem cells with the most potential for tooth tissue regeneration. However, the culture of hDPSCs in vitro for tissue engineering is challenging, as cells may proliferate slowly or/and differentiate poorly in vivo. Dynamic three‑dimensional (3D) simulated microgravity (SMG) created using the rotary cell culture system is considered to an effective tool, which contributes to several cell functions...
December 14, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28000362/modified-porous-scaffolds-of-silk-fibroin-with-mimicked-microenvironment-based-on-decellularized-pulp-fibronectin-for-designed-performance-biomaterials-in-maxillofacial-bone-defect
#11
Supaporn Sangkert, Suttatip Kamonmattayakul, Wen Lin Chai, Jirut Meesane
Maxillofacial bone defect is a critical problem for many patients. In severe cases, the patients need an operation using a biomaterial replacement. Therefore, to design performance biomaterials is a challenge for materials scientists and maxillofacial surgeons. In this research, porous silk fibroin scaffolds with mimicked microenvironment based on decellularized pulp and fibronectin were created as for bone regeneration. Silk fibroin scaffolds were fabricated by freeze-drying before modification with three different components: decellularized pulp, fibronectin, and decellularized pulp/fibronectin...
December 21, 2016: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/27998031/could-the-application-of-bioactive-molecules-improve-vital-pulp-therapy-success-a-systematic-review
#12
REVIEW
Wellington Luiz de Oliveira da Rosa, Tiago Machado da Silva, Flávio Fernando Demarco, Evandro Piva, Adriana Fernandes da Silva
This study aimed to systematically review the literature of animal studies to evaluate whether bioactive dentin proteins could improve vital pulp therapy success. The review is reported in accordance with the PRISMA Statement. Two reviewers independently conducted a literature search of seven databases: PubMed (Medline), Lilacs, IBECS, BBO, Web of Science, Scopus and SciELO. Animal experiments in which bioactive dentin proteins were applied directly or indirectly to the pulp tissue were included. Data regarding the characteristics of the proteins evaluated, the delivery systems used and the main findings from each study were tabulated to assess the outcomes of interest (tertiary dentin formation, inflammatory response, intratubular mineralization)...
December 20, 2016: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/27989823/porous-microscaffolds-for-3d-culture-of-dental-pulp-mesenchymal-stem-cells
#13
Ronak S Bhuptani, Vandana B Patravale
The collective power of stem cells due to their evident advantages is incessantly investigated in regenerative medicine to be the next generation exceptional remedy for tissue regeneration and treatment of diseases. Stem cells are highly sensitive and a 3D culture environment is a requisite for its successful transplantation and integration with tissues. Porous microscaffolds can create a 3D microenvironment for growing stems cells, controlling their fate both in vitro and in vivo. In the present study, interconnected porous PLGA microscaffolds were fabricated, characterized and employed to propagate human dental pulp mesenchymal stem cells (DPMSCs) in vitro...
October 27, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27986102/regenerative-endodontic-procedures-a-perspective-from-stem-cell-niche-biology
#14
REVIEW
Manuel Marí-Beffa, Juan José Segura-Egea, Aránzazu Díaz-Cuenca
INTRODUCTION: Endodontics uses cell therapy strategies to treat pulpal and periapical diseases. During these therapies, surgeons aim to reconstruct the natural microenvironments that regulate the activity of dental stem cells. METHODS: We searched for more than 400 articles in PubMed using key words from regenerative endodontics and dental stem cell biology. In 268 articles, we reviewed what factors may influence histologic results after preclinical dental treatments that use regenerative endodontic procedures after pulpectomy...
January 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/27973697/enhanced-regeneration-potential-of-mobilized-dental-pulp-stem-cells-from-immature-teeth
#15
Hidenori Nakayama, Koichiro Iohara, Yuki Hayashi, Yuta Okuwa, Kurita Kenichi, Misako Nakashima
OBJECTIVES: We have previously demonstrated that dental pulp stem cell (DPSCs) isolated from mature teeth by granulocyte-colony stimulating factor (G-CSF)-induced mobilization method can enhance angiogenesis/vasculogenesis and improve pulp regeneration when compared with colony-derived DPSCs. However, the efficacy of this method in immature teeth with root-formative stage has never been investigated. Therefore, the aim of this study is to examine the stemness, biological characteristics and regeneration potential in mobilized-DPSCs compared with colony-derived DPSCs from immature teeth...
December 14, 2016: Oral Diseases
https://www.readbyqxmd.com/read/27966263/molecular-and-microarray-based-analysis-of-diversity-among-resting-and-osteogenically-induced-porcine-mesenchymal-stromal-cells-of-several-tissue-origin
#16
Samet Bayraktar, Pascal Jungbluth, René Deenen, Jan Grassmann, Johannes Schneppendahl, Daphne Eschbach, Armin Scholz, Joachim Windolf, Christoph V Suschek, Vera Grotheer
Mesenchymal stromal cells (MSC) play a pivotal role in modern therapeutic approaches in bone healing disorders. Although bone marrow-derived MSC are most frequently used, the knowledge that many other adult tissues represent promising sources for potent MSC has gained acceptance. In the present study, the osteogenic differentiation potential of porcine skin fibroblasts (FB) as well as bone marrow- (BMSC), adipose tissue- (ASC), and dental pulp-derived stromal cells (DSC) were evaluated. However, additional application of BMP-2 significantly elevated the delayed osteogenic differentiation capacity of ASC and FB cultures...
December 14, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27943583/human-dental-pulp-stem-cells-expressing-stro-1-c-kit-and-cd34-markers-in-peripheral-nerve-regeneration
#17
Gianluca Carnevale, Alessandra Pisciotta, Massimo Riccio, Laura Bertoni, Sara De Biasi, Lara Gibellini, Alessio Zordani, Gian Maria Cavallini, Giovanni Battista La Sala, Giacomo Bruzzesi, Adriano Ferrari, Andrea Cossarizza, Anto de Pol
Peripheral nerve injuries are a commonly encountered clinical problem and often result in long-term functional defects. The application of stem cells able to differentiate in Schwann cells-like cells in vitro and in vivo, could represent an attractive therapeutic approach for the treatment of nerve injuries. Further, stem cells sources sharing the same embryological origin of Schwann cells might be considered a suitable tool. The aim of this study was to demonstrate the ability of a neuroectodermal subpopulation of human STRO-1+/c-Kit+/CD34+ DPSCs, expressing P75(NTR) , Nestin and SOX-10, to differentiate into Schwann cells-like cells in vitro and to promote axonal regeneration in vivo, which led to functional recovery as measured by sustained gait improvement, in animal rat model of peripheral nerve injury...
December 11, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27939739/characterization-of-a-vascular-endothelial-growth-factor-loaded-bioresorbable-delivery-system-for-pulp-regeneration
#18
Mamatha Yadlapati, Claudia Biguetti, Franco Cavalla, Francisco Nieves, Christopher Bessey, Pedram Bohluli, Gustavo Pompermaier Garlet, Ariadne Letra, Walid D Fakhouri, Renato Menezes Silva
INTRODUCTION: Vascular endothelial growth factor (VEGF) is a signal protein that stimulates angiogenesis and vasculogenesis and has been used in tissue regeneration and pulp regeneration experimental models. The purpose of this study was to develop a delivery system composed of a biodegradable fiber and controlled release of VEGF to promote cell viability and secure an adequate blood supply for the survival of human stem cells of the apical papilla (SCAP) favoring endodontic regenerative procedures...
January 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/27939733/mesoporous-calcium-silicate-nanoparticles-with-drug-delivery-and-odontogenesis-properties
#19
Ching-Yuang Huang, Tsui-Hsien Huang, Chia-Tze Kao, Yuan-Haw Wu, Wan-Chen Chen, Ming-You Shie
INTRODUCTION: Calcium silicate (CS) -based materials play an important role in the development of endodontic materials that induce bone/cementum tissue regeneration and inhibit bacterial viability. The aim of this study was to prepare novel mesoporous CS (MesoCS) nanoparticles that have osteogenic, drug delivery, and antibacterial characteristics for endodontic materials and also have an excellent ability to develop apatite mineralization. METHODS: The MesoCS nanoparticles were prepared using sol-gel methods...
January 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/27934940/accelerated-craniofacial-bone-regeneration-through-dense-collagen-gel-scaffolds-seeded-with-dental-pulp-stem-cells
#20
Frédéric Chamieh, Anne-Margaux Collignon, Benjamin R Coyac, Julie Lesieur, Sandy Ribes, Jérémy Sadoine, Annie Llorens, Antonino Nicoletti, Didier Letourneur, Marie-Laure Colombier, Showan N Nazhat, Philippe Bouchard, Catherine Chaussain, Gael Y Rochefort
Therapies using mesenchymal stem cell (MSC) seeded scaffolds may be applicable to various fields of regenerative medicine, including craniomaxillofacial surgery. Plastic compression of collagen scaffolds seeded with MSC has been shown to enhance the osteogenic differentiation of MSC as it increases the collagen fibrillary density. The aim of the present study was to evaluate the osteogenic effects of dense collagen gel scaffolds seeded with mesenchymal dental pulp stem cells (DPSC) on bone regeneration in a rat critical-size calvarial defect model...
December 9, 2016: Scientific Reports
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