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Pulp regeneration

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https://www.readbyqxmd.com/read/27914008/igf1r-dental-pulp-stem-cells-enhanced-neuroplasticity-in-hypoxia-ischemia-model
#1
Hsiao-Yu Chiu, Chen-Huan Lin, Chung Y Hsu, John Yu, Chia-Hung Hsieh, Woei-Cherng Shyu
Until now, the surface markers of multipotent mesenchymal stem cells (MSCs) had not been fully identified. Here, we found that the IGF1 receptor (IGF1R), regarded as a pluripotent marker of embryonic stem cells (ESCs), was also expressed in human dental pulp derived-mesenchymal stem cells (hDSCs), which displayed a potential for both self-renewal and multipotency. hDSC-secreted IGF1 interacted with IGF1R through an autocrine signaling pathway to maintain this self-renewal and proliferation potential. Stereotaxic implantation of immunosorted IGF1R(+) hDSCs in rats with neonatal hypoxia-ischemia (NHI) promoted neuroplasticity, improving the neurological outcome by increasing expression of the anti-apoptotic protein Bcl-2, which enhanced both neurogenesis and angiogenesis...
December 2, 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/27912814/biological-molecules-for-the-regeneration-of-the-pulp-dentin-complex
#2
REVIEW
Sahng G Kim
Regenerative endodontic treatment has yielded excellent clinical outcomes, but only several animal studies have shown the robust regeneration of the pulp-dentin complex. The biological molecules, if properly delivered, can enkindle regeneration of dental pulp and dentin rather than repair with tissues of periodontal origin. This review details the biological significance of regenerating the pulp-dentin complex, the effects of biological cues in pulp regeneration, and the delivery strategies of biological molecules to enhance the outcomes of regenerative endodontic therapy...
January 2017: Dental Clinics of North America
https://www.readbyqxmd.com/read/27901202/in-vivo-performance-of-different-scaffolds-for-dental-pulp-stem-cells-induced-for-odontogenic-differentiation
#3
Cigdem Atalayin, Huseyin Tezel, Taner Dagci, Nefise Ulku Karabay Yavasoglu, Gulperi Oktem, Timur Kose
This study was designed to determine the in vivo performance of three different materials as scaffolds for dental pulp stem cells (DPSC) undergoing induced odontogenic differentiation. An odontogenic medium modified by the addition of recombinant human bone morphogenetic protein 2 was used in the experimental groups to induce differentiation. Mesenchymal stem cell medium was used in the control groups. DPSC were transplanted onto the backs of mice via three scaffolds: copolymer of L-lactide and DL-lactide (PLDL), copolymer of DL-lactide (PDL) and hydroxyapatite tricalcium phosphate (HA/TCP)...
November 28, 2016: Brazilian Oral Research
https://www.readbyqxmd.com/read/27890225/applications-of-mesenchymal-stem-cells-in-oral-and-craniofacial-regeneration
#4
REVIEW
Pasha Shakoori, Quanzhou Zhang, Anh D Le
The field of tissue engineering and regenerative medicine has been rapidly expanded through multidisciplinary integration of research and clinical practice in response to unmet clinical needs for reconstruction of dental, oral, and craniofacial structures. The significance of the various types of stem cells, specifically mesenchymal stem cells derived from the orofacial tissues, ranging from dental pulp stem cells to periodontal ligament stem cells to mucosa/gingiva has been thoroughly investigated and their applications in tissue regeneration are outlined in this article...
February 2017: Oral and Maxillofacial Surgery Clinics of North America
https://www.readbyqxmd.com/read/27877132/immunophenotyping-reveals-the-diversity-of-human-dental-pulp-mesenchymal-stromal-cells-in-vivo-and-their-evolution-upon-in-vitro-amplification
#5
Maxime Ducret, Hugo Fabre, Olivier Degoul, Gianluigi Atzeni, Colin McGuckin, Nico Forraz, Frédéric Mallein-Gerin, Emeline Perrier-Groult, Brigitte Alliot-Licht, Jean-Christophe Farges
Mesenchymal stromal/stem cells (MSCs) from human dental pulp (DP) can be expanded in vitro for cell-based and regenerative dentistry therapeutic purposes. However, their heterogeneity may be a hurdle to the achievement of reproducible and predictable therapeutic outcomes. To get a better knowledge about this heterogeneity, we designed a flow cytometric strategy to analyze the phenotype of DP cells in vivo and upon in vitro expansion with stem cell markers. We focused on the CD31(-) cell population to exclude endothelial and leukocytic cells...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27871783/human-dental-pulp-stem-cells-suppress-alloantigen-induced-immunity-by-stimulating-t-cells-to-release-transforming-growth-factor-beta
#6
Kyu Hwan Kwack, Jung Min Lee, Sang Hyuk Park, Hyeon Woo Lee
INTRODUCTION: Human dental pulp stem cells (hDPSCs) are ideal candidates for regenerating damaged dental tissue. To examine the possibility that hDPSCs may be used to regenerate pulp, we tested their in vitro effects on acute allogeneic immune responses. METHODS: A peripheral blood mononuclear cell (PBMC) proliferation assay and immunoglobulin (Ig) production assay were performed to evaluate the immunosuppressive properties of hDPSCs. RESULTS: The mixed lymphocyte reaction was suppressed by incubation with hDPSCs...
November 18, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27871176/in-vivo-angiogenic-capacity-of-stem-cells-from-human-exfoliated-deciduous-teeth-with-human-umbilical-vein-endothelial-cells
#7
Ji-Hye Kim, Gee-Hye Kim, Jae-Won Kim, Hee Jang Pyeon, Jae Cheoun Lee, Gene Lee, Hyun Nam
Dental pulp is a highly vascularized tissue requiring adequate blood supply for successful regeneration. In this study, we investigated the functional role of stem cells from human exfoliated deciduous teeth (SHEDs) as a perivascular source for in vivo formation of vessel-like structures. Primarily isolated SHEDs showed mesenchymal stem cell (MSC)-like characteristics including the expression of surface antigens and in vitro osteogenic and adipogenic differentiation potentials. Moreover, SHEDs were positive for NG2, α-smooth muscle actin (SMA), platelet-derived growth factor receptor beta (PDGFRβ), and CD146 as pericyte markers...
November 30, 2016: Molecules and Cells
https://www.readbyqxmd.com/read/27857762/scaffolds-in-regenerative-endodontics-a-review
#8
REVIEW
Kinjal M Gathani, Srinidhi Surya Raghavendra
Root canal therapy has enabled us to save numerous teeth over the years. The most desired outcome of endodontic treatment would be when diseased or nonvital pulp is replaced with healthy pulp tissue that would revitalize the teeth through regenerative endodontics. 'A search was conducted using the Pubmed and MEDLINE databases for articles with the criteria 'Platelet rich plasma', 'Platelet rich fibrin', 'Stem cells', 'Natural and artificial scaffolds' from 1982-2015'. Tissues are organized as three-dimensional structures, and appropriate scaffolding is necessary to provide a spatially correct position of cell location and regulate differentiation, proliferation, or metabolism of the stem cells...
September 2016: Dental Research Journal
https://www.readbyqxmd.com/read/27848099/dental-pulp-tissue-engineering-of-pulpotomized-rat-molars-with-bone-marrow-mesenchymal-stem-cells
#9
Takafumi Ito, Tomoatsu Kaneko, Yukiko Sueyama, Reika Kaneko, Takashi Okiji
The major goal of dental pulp tissue engineering is to enable the healing of inflamed tissue or to replace necrotic pulp tissue with newly formed dental pulp tissue. Here, we report a protocol for pulp tissue engineering in vivo in pulpotomized rat teeth using constructs of rat bone marrow mesenchymal stem cells, preformed biodegradable scaffolds, and hydrogel. The constructs were implanted into pulpotomized pulp chambers for 3, 7, or 14 days. At 3 days, cells were located mainly along the preformed scaffolds...
November 15, 2016: Odontology
https://www.readbyqxmd.com/read/27818690/stem-cells-of-dental-origin-current-research-trends-and-key-milestones-towards-clinical-application
#10
REVIEW
Athina Bakopoulou, Imad About
Dental Mesenchymal Stem Cells (MSCs), including Dental Pulp Stem Cells (DPSCs), Stem Cells from Human Exfoliated Deciduous teeth (SHED), and Stem Cells From Apical Papilla (SCAP), have been extensively studied using highly sophisticated in vitro and in vivo systems, yielding substantially improved understanding of their intriguing biological properties. Their capacity to reconstitute various dental and nondental tissues and the inherent angiogenic, neurogenic, and immunomodulatory properties of their secretome have been a subject of meticulous and costly research by various groups over the past decade...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27793148/pdgfr%C3%AE-c-kit-pulp-cells-are-odontoblastic-progenitors-capable-of-producing-dentin-like-structure-in-vitro-and-in-vivo
#11
Shiwei Cai, Wenjian Zhang, Wei Chen
BACKGROUND: Successful pulp regeneration depends on identification of pulp stem cells capable of differentiation under odontoblastic lineage and producing pulp-dentinal like structure. Recent studies demonstrate that platelet-derived growth factor (PDGF) plays an important role in damage repair and tissue regeneration. The aim of this study was to identify a subpopulation of dental pulp cells responsive to PDGF and with dentin regeneration potential. METHODS: Pulp tissues were isolated from 12 freshly extracted human impacted third molars...
October 28, 2016: BMC Oral Health
https://www.readbyqxmd.com/read/27790081/regenerative-dentistry-animal-model-for-regenerative-endodontology
#12
Nisarat Ruangsawasdi, Matthias Zehnder, Raphael Patcas, Chafik Ghayor, Franz E Weber
BACKGROUND: Ectopic tissue has been observed frequently in human root canal specimens when cell homing studies were performed at the dorsum of rodents. In contrast, pulp-like tissue formed when immature teeth were implanted on top of the rat calvaria. It was surmised, yet not tested, that the implantation site might affect tissue ingrowth. METHODS: Four root sections from human immature molars cleaned with 5% sodium hypochlorite (NaOCl) followed by 17% ethylenediaminetetraacetic acid (EDTA) were implanted per rat (n = 5)...
September 2016: Transfusion Medicine and Hemotherapy
https://www.readbyqxmd.com/read/27788772/a-new-method-to-develop-human-dental-pulp-cells-and-platelet-rich-fibrin-complex
#13
Xuan He, Wen-Xia Chen, Guifei Ban, Wei Wei, Jun Zhou, Wen-Jin Chen, Xian-Yu Li
INTRODUCTION: Platelet-rich fibrin (PRF) has been used as a scaffold material in various tissue regeneration studies. In the previous methods to combine seed cells with PRF, the structure of PRF was damaged, and the manipulation time in vitro was also increased. The objective of this in vitro study was to explore an appropriate method to develop a PRF-human dental pulp cell (hDPC) complex to maintain PRF structure integrity and to find out the most efficient part of PRF. METHODS: The PRF-hDPC complex was developed at 3 different time points during PRF preparation: (1) the before centrifugation (BC) group, the hDPC suspension was added to the venous blood before blood centrifugation; (2) the immediately after centrifugation (IAC) group, the hDPC suspension was added immediately after blood centrifugation; (3) the after centrifugation (AC) group, the hDPC suspension was added 10 minutes after blood centrifugation; and (4) the control group, PRF without hDPC suspension...
November 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27785631/odontogenic-differentiation-and-biomineralization-potential-of-dental-pulp-stem-cells-inside-mg-based-bioceramic-scaffolds-under-low-level-laser-treatment
#14
Anna Theocharidou, Athina Bakopoulou, Eleana Kontonasaki, Eleni Papachristou, Christina Hadjichristou, Maria Bousnaki, George Theodorou, Lambrini Papadopoulou, Nikolaos Kantiranis, Konstantinos Paraskevopoulos, Petros Koidis
This study aimed to investigate the potential of low-level laser irradiation (LLLI) to promote odontogenic differentiation and biomineralization by dental pulp stem cells (DPSCs) seeded inside bioceramic scaffolds. Mg-based, Zn-doped bioceramic scaffolds, synthesized by the sol-gel technique, were spotted with DPSCs and exposed to LLLI at 660 nm with maximum output power of 140 mw at fluencies (a) 2 and 4 J/cm(2) to evaluate cell viability/proliferation by the MTT assay and (b) 4 J/cm(2) to evaluate cell differentiation, using real-time PCR (expression of odontogenic markers) and a p-nitrophenylphosphate (pNPP)-based assay for alkaline phosphatase (ALP) activity measurement...
October 26, 2016: Lasers in Medical Science
https://www.readbyqxmd.com/read/27776883/gene-expression-profiling-and-molecular-signaling-of-various-cells-in-response-to-tricalcium-silicate-cements-a-systematic-review
#15
Elanagai Rathinam, Sivaprakash Rajasekharan, Ravi Teja Chitturi, Heidi Declercq, Luc Martens, Peter De Coster
INTRODUCTION: The aim of this study was to present a systematic review investigating the gene expression of various cells (other than dental pulp cells) in response to different variants of tricalcium silicate cements (TSCs). METHODS: A systematic search of the literature was performed by 2 independent reviewers followed by article selection and data extraction. Studies analyzing any cell type except dental pulp stem cells and any variant of tricalcium silicate cement either as the experimental or as the control group were included...
October 21, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27762658/effects-of-stem-cell-factor-on-cell-homing-during-functional-pulp-regeneration-in-human-immature-teeth
#16
Nisarat Ruangsawasdi, Matthias Zehnder, Raphael Patcas, Chafik Ghayor, Barbara Siegenthaler, Bebeka Gjoksi, Franz E Weber
Conventional root canal treatment in immature permanent teeth can lead to early tooth loss in children because root formation is discontinued. We investigated whether the stem cell factor (SCF) could facilitate cell homing in the pulpless immature root canal and promote regeneration of a functional pulp. In vitro, human mesenchymal stem cells (hMSCs) were exposed to SCF at various concentrations for assessing cell migration, proliferation, and differentiation toward odonto/osteoblasts by 3D-chemotaxis slides, WST-1 assay, and alkaline phosphatase activity, respectively...
November 22, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27728810/exosomes-as-biomimetic-tools-for-stem-cell-differentiation-applications-in-dental-pulp-tissue-regeneration
#17
Chun-Chieh Huang, Raghuvaran Narayanan, Satish Alapati, Sriram Ravindran
Achieving and maintaining safe and reliable lineage specific differentiation of stem cells is important for clinical translation of tissue engineering strategies. In an effort to circumvent the multitude of problems arising from the usage of growth factors and growth factor delivery systems, we have explored the use of exosomes as biomimetic tools to induce stem cell differentiation. Working on the hypothesis that cell-type specific exosomes can trigger lineage-specific differentiation of stem cells, we have evaluated the potential of exosomes derived from dental pulp cells cultured on under growth and odontogenic differentiation conditions to induce odontogenic differentiation of naïve human dental pulp stem cells (DPSCs) and human bone marrow derived stromal cells (HMSCs) in vitro and in vivo...
December 2016: Biomaterials
https://www.readbyqxmd.com/read/27725267/regulatory-roles-of-micrornas-in-human-dental-tissues
#18
Amer Sehic, Amela Tulek, Cuong Khuu, Minou Nirvani, Lars Peter Sand, Tor Paaske Utheim
MicroRNAs (miRNAs) are a class of small, non-coding RNAs that provide an efficient pathway for regulation of gene expression at a post-transcriptional level. Tooth development is regulated by a complex network of cell-cell signaling during all steps of organogenesis. Most of the congenital dental defects in humans are caused by mutations in genes involved in developmental regulatory networks. Whereas the developmental morphological stages of the tooth development already are thoroughly documented, the implicated genetic network is still under investigation...
October 7, 2016: Gene
https://www.readbyqxmd.com/read/27723266/effects-of-tgf-%C3%AE-1-on-plasminogen-activation-in-human-dental-pulp-cells-role-of-alk5-smad2-tak1-and-mek-erk-signaling
#19
Mei-Chi Chang, Hsiao-Hua Chang, Po-Shuan Lin, Yu-An Huang, Chiu-Po Chan, Yi-Ling Tsai, Shen-Yang Lee, Po-Yuan Jeng, Han-Yueh Kuo, Sin-Yuet Yeung, Jiiang-Huei Jeng
Transforming growth factor-β1 (TGF-β1) plays an important role in the pulpal repair and dentinogenesis. Plasminogen activation (PA) system regulates extracellular matrix turnover. In this study, we investigated the effects of TGF-β1 on PA system of dental pulp cells and its signaling pathways. Dental pulp cells were treated with different concentrations of TGF-β1. MTT assay, reverse transcription-polymerase chain reaction (RT-PCR), western blotting and enzyme-linked immunosorbant assay (ELISA) were used to detect the effect of TGF-β1 on cell viability, mRNA and protein expression of urokinase-type plasminogen activator (uPA), uPA receptor (uPAR), plasminogen activator inhibitor-1 (PAI-1) as well as their secretion...
October 9, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27722231/strontium-folate-loaded-biohybrid-scaffolds-seeded-with-dental-pulp-stem-cells-induce-in-vivo-bone-regeneration-in-critical-sized-defects
#20
Marcela Martin-Del-Campo, Raul Rosales-Ibañez, Keila Alvarado, Jose G Sampedro, Christian A Garcia-Sepulveda, Sanjukta Deb, Julio San Román, Luis Rojo
Strontium folate (SrFO) is a recently developed bone promoting agent with interest in medical and pharmaceutical fields due to its improved features in comparison to current strontium based therapies for osteoporosis and other bone diseases. In this work SrFO derivative was synthesized and loaded into biohybrid scaffolds obtained through lyophilisation of semi-interpenetrating networks of chitosan polyethylene glycol dimethacrylate and beta tri-calcium phosphate (βTCP) fabricated using free radical polymerization...
October 18, 2016: Biomaterials Science
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