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https://www.readbyqxmd.com/read/28531660/carbon-black-aggregates-cause-endothelial-dysfunction-by-activating-rock
#1
Junyan Yan, Chia-Hsiang Lai, Shih-Chun Candice Lung, Wen-Cheng Wang, Chih-Ching Huang, Guan-Wen Chen, Guangli Suo, Cheng-Tai Choug, Chia-Hua Lin
Carbon black nanoparticles (CBNs) have been associated with the progression of atherosclerosis. CBNs normally enter the bloodstream and crosslink together to form agglomerates. However, most studies have used nano-sized CB particles to clarify the involvement of CBN exposure in CBN-induced endothelial dysfunction. Herein, we studied endothelial toxicity of CBN aggregates (CBA) to human EA.hy926 vascular cells. Cell viability, lactate dehydrogenase leakage, and oxidative stress were affected by the highest concentration of CBA...
May 15, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28529605/mutual-regulation-of-mir-199a-5p-and-hif-1%C3%AE-modulates-the-warburg-effect-in-hepatocellular-carcinoma
#2
Binkui Li, Liru He, Dinglan Zuo, Wei He, Yongjin Wang, Yuanping Zhang, Wenwu Liu, Yunfei Yuan
The Warburg effect is one of the major metabolic changes of cancer cells, which characterized by high level of glycolysis even in the presence of oxygen. However, the role of microRNAs (miRNAs) in regulating the glycolytic switch in cancer cells has not been well explored. In this study, we demonstrated that miR-199a-5p acts as a suppressor of the Warburg effect in hepatocellular carcinoma (HCC). MiR-199a-5p directly targets the 3'-untranslated region (UTR) of hypoxia-inducible factor-1α (HIF-1α), thereby suppressing glucose uptake, lactate production, cell growth, and expression of HIF-1α downstream glycolytic genes of HCC cells...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28528240/negative-correlation-between-csf-lactate-levels-and-moca-scores-in-male-chinese-subjects
#3
Haijun Wang, Xiaohua Tan, Jinzhong Xu, Hui Li, Meiling Wang, Suling Chen, Xiaoyu Yang, Yanlong Liu, Fan Wang
Lactate is a product of glycolysis by astrocytes and can be generated as a primary metabolic energy source by neurons. Lactate plays multifunctional role in human brain energy metabolism and cognitive function. However, no direct evidence demonstrated the link between lactate and cognition in normal condition. In the present study, concentrations of lactate in cerebrospinal fluid (CSF) and peripheral blood were measured to investigate the association of lactate with cognitive ability, which was assessed by Montreal Cognitive Assessment (MoCA), in 99 male Chinese subjects...
May 12, 2017: Psychiatry Research
https://www.readbyqxmd.com/read/28528091/antiproliferative-activity-of-ontario-grown-onions-against-colorectal-adenocarcinoma-cells
#4
Abdulmonem I Murayyan, Cynthya M Manohar, Gordon Hayward, Suresh Neethirajan
Cancer is the leading cause of mortality in Canada and other industrialized nations; the development of new/improved cancer therapies is desperately needed and continues to be a major focus of cancer research. Flavonoids, which are found in high levels in onions, have been shown to exert antiproliferative and potentially anti-cancer activities. To test their therapeutic potential, we assessed the antiproliferative, cytotoxic, apoptosis-inducing, and anti-migratory activities of five onion varieties grown in Ontario against human adenocarcinoma (Caco-2) cells...
June 2017: Food Research International
https://www.readbyqxmd.com/read/28527013/central-activation-metabolites-and-calcium-handling-during-fatigue-with-repeated-maximal-isometric-contractions-in-human-muscle
#5
Simeon P Cairns, Luke A G Inman, Caroline P MacManus, Ingrid G L van de Port, Patricia A Ruell, Jeanette M Thom, Martin W Thompson
PURPOSE: To determine the roles of calcium (Ca(2+)) handling by sarcoplasmic reticulum (SR) and central activation impairment (i.e., central fatigue) during fatigue with repeated maximal voluntary isometric contractions (MVC) in human muscles. METHODS: Contractile performance was assessed during 3 min of repeated MVCs (7-s contraction, 3-s rest, n = 17). In ten participants, in vitro SR Ca(2+)-handling, metabolites, and fibre-type composition were quantified in biopsy samples from quadriceps muscle, along with plasma venous [K(+)]...
May 19, 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/28526448/the-catechol-o-methyltransferase-inhibitors-tolcapone-and-entacapone-uncouple-and-inhibit-the-mitochondrial-respiratory-chain-in-heparg-cells
#6
David Grünig, Andrea Felser, Jamal Bouitbir, Stephan Krähenbühl
The catechol-O-methyltransferase inhibitor tolcapone causes hepatotoxicity and mitochondrial damage in animal models. We studied the interaction of tolcapone with mitochondrial respiration in comparison to entacapone in different experimental models. In HepaRG cells (human cell-line), tolcapone decreased the ATP content (estimated IC50 100±15μM) and was cytotoxic (estimated IC50 333±45μM), whereas entacapone caused no cytotoxicity and no ATP depletion up to 200μM. Cytochrome P450 induction did not increase the toxicity of the compounds...
May 16, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28524013/probiotic-supplementation-effects-on-milk-fatty-acid-profile-in-ewes
#7
Shahab Payandeh, Farokh Kafilzadeh, Manuela Juárez, Miguel Angel de la Fuente, Darab Ghadimi, Andrés L Martínez Marín
We hypothesised that probiotic feeding would alter the fatty acid (FA) profile of sheep's milk. Sixteen lactating ewes, kept under the same feeding and management practices, were randomly allocated to receive either a control diet or the same diet supplemented with a commercial multi-strain bacterial probiotic. Milk fat FA contents were monitored fortnightly for eight consecutive weeks from 14 d after lambing. Probiotic supplementation increased the contents of butyric and caproic acids in milk fat and had no negative effects on other relevant FA from the human's health point of view (i...
May 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/28523959/content-fat-and-calorie-of-human-milk-is-affected-by-interactions-between-maternal-age-and-body-mass-index
#8
Won-Ho Hahn, Tchaewon Jeong, Suyeon Park, Seunghyun Song, Nam Mi Kang
PURPOSE: We evaluated the association between macronutrients of human milk (HM), and interactions between maternal age and body mass index(BMI) in matched conditions. MATERIAL AND METHODS: Totally, 80 HM samples were collected from healthy breast-feeding mothers at fourth week of lactation. HM macronutrients and maternal data were analyzed. Mothers were subgrouped into four groups by maternal age (20 s/30 s) and BMI (overweight/normal). RESULTS: Two-way ANOVA revealed significant interactions between age and BMI to affect macronutrients; fat, carbohydrate, and calories...
May 19, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28523535/self-protection-against-triptolide-induced-toxicity-in-human-hepatic-cells-via-nrf2-are-nqo1-pathway
#9
Ling-Ling Zhou, Cong Zhou, Xiao-Wen Liang, Zhe Feng, Zhang-Pu Liu, Hao-Lu Wang, Xue-Ping Zhou
OBJECTIVE: To find the signaling pathway of triptolide (TP)-induced liver injury and to reveal whether NF-E2-related factor 2 (Nrf2) plays an important role in cellular self-protection. METHODS: The L-02 and HepG2 cells were cultured and treated with various concentrations of TP. The cell viability was observed, and the cell medium was collected for detecting the aspartate aminotransferase (ALT), alanine aminotransferase (AST), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and L-glutathione production (GSH) levels...
May 18, 2017: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28522001/cytotoxic-effect-of-a-mannogalactoglucan-extracted-from-agaricus-bisporus-on-hepg2-cells
#10
Amanda do Rocio Andrade Pires, Andrea Caroline Ruthes, Silvia Maria Suter Correia Cadena, Marcello Iacomini
A mannogalactoglucan (RK2-Ab; Mw 1.8×10(4)gmol(-1)) composed by Man (27.3%), Gal (24.4%) and Glc (48.3%) was extracted and characterized from Agaricus bisporus, and its biological activity was evaluated on human hepatocarcinoma cells (HepG2). The partially-O-methylated alditol acetates together with the NMR data suggest the main chain to be composed of α-d-Galp (32.8%) and β-d-Glcp (37.0%) units (1→6)-linked, with β-d-Manp (14.6%), as non-reducing end units, substituting the side chains at O-2 (α-d-Galp units; 3...
August 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28521193/issues-raised-by-the-reference-doses-for-perfluorooctane-sulfonate-and-perfluorooctanoic-acid
#11
Zhaomin Dong, Md Mezbaul Bahar, Joytishna Jit, Bruce Kennedy, Brian Priestly, Jack Ng, Dane Lamb, Yanju Liu, Luchun Duan, Ravi Naidu
On 25th May 2016, the U.S. EPA released reference doses (RfDs) for Perfluorooctane Sulfonate (PFOS) and Perfluorooctanoic Acid (PFOA) of 20ng/kg/day, which were much more conservative than previous values. These RfDs rely on the choices of animal point of departure (PoD) and the toxicokinetics (TK) model. At this stage, considering that the human evidence is not strong enough for RfD determination, using animal data may be appropriate but with more uncertainties. In this article, the uncertainties concerning RfDs from the choices of PoD and TK models are addressed...
May 15, 2017: Environment International
https://www.readbyqxmd.com/read/28520579/the-use-of-an-acellular-oxygen-carrier-in-a-human-liver-model-of-normothermic-machine-perfusion
#12
Richard W Laing, Ricky H Bhogal, Lorraine Wallace, Yuri Boteon, Desley A H Neil, Amanda Smith, Barney T F Stephenson, Andrea Schlegel, Stefan G Hübscher, Darius F Mirza, Simon C Afford, Hynek Mergental
BACKGROUND: Normothermic machine perfusion of the liver (NMP-L) is a novel technique that preserves liver grafts under near-physiological conditions whilst maintaining their normal metabolic activity. This process requires an adequate oxygen supply, typically delivered by packed red blood cells (RBC). We present the first experience using an acellular hemoglobin-based oxygen carrier (HBOC) Hemopure in a human model of NMP-L. METHODS: Five discarded high-risk human livers were perfused with HBOC-based perfusion fluid and matched to 5 RBC-perfused livers...
May 18, 2017: Transplantation
https://www.readbyqxmd.com/read/28516269/%C3%AE-alanine-intercede-metabolic-recovery-for-amelioration-of-human-cervical-and-renal-tumors
#13
Muthuraman Pandurangan, Gansukh Enkhtaivan, Bhupendra Mistry, Rahul V Patel, Sohyun Moon, Doo Hwan Kim
β-Alanine is a non-essential amino acid and presents as a major component of various sports supplements. It is a non-proteogenic amino acid, formed in vivo by degradation of carnosine, anserine, balenine, and dihydrouracil. The present study was aimed at investigating the anti-tumor effects of β-alanine in renal and cervical tumor cells. Sulforhodamine-B assay and flow cytometric analysis were used to measure cell viability. Lactate dehydrogenase (LDH) expression was analyzed using FITC-conjugated fluorescent antibody...
May 17, 2017: Amino Acids
https://www.readbyqxmd.com/read/28514652/thymidine-catabolism-as-a-metabolic-strategy-for-cancer-survival
#14
Sho Tabata, Masatatsu Yamamoto, Hisatsugu Goto, Akiyoshi Hirayama, Maki Ohishi, Takuya Kuramoto, Atsushi Mitsuhashi, Ryuji Ikeda, Misako Haraguchi, Kohichi Kawahara, Yoshinari Shinsato, Kentaro Minami, Atsuro Saijo, Masaki Hanibuchi, Yasuhiko Nishioka, Saburo Sone, Hiroyasu Esumi, Masaru Tomita, Tomoyoshi Soga, Tatsuhiko Furukawa, Shin-Ichi Akiyama
Thymidine phosphorylase (TP), a rate-limiting enzyme in thymidine catabolism, plays a pivotal role in tumor progression; however, the mechanisms underlying this role are not fully understood. Here, we found that TP-mediated thymidine catabolism could supply the carbon source in the glycolytic pathway and thus contribute to cell survival under conditions of nutrient deprivation. In TP-expressing cells, thymidine was converted to metabolites, including glucose 6-phosphate, lactate, 5-phospho-α-D-ribose 1-diphosphate, and serine, via the glycolytic pathway both in vitro and in vivo...
May 16, 2017: Cell Reports
https://www.readbyqxmd.com/read/28513863/relative-contributions-of-maternal-western-type-high-fat-high-sugar-diets-and-maternal-obesity-to-altered-metabolic-function-in-pregnancy
#15
Elena Zambrano, Peter W Nathanielsz
Human epidemiological and experimental animal studies have shown that maternal obesity (MO) combined with high energy Western-style high fat-high sugar (HFHS) obesogenic diets can have adverse effects on mothers and fetuses during pregnancy and lactation and predispose offspring to later life metabolic dysfunction. This article is protected by copyright. All rights reserved.
May 17, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28513622/human-milk-insulin-is-related-to-maternal-plasma-insulin-and-bmi-but-other-components-of-human-milk-do-not-differ-by-bmi
#16
B E Young, Z Patinkin, C Palmer, B de la Houssaye, L A Barbour, T Hernandez, J E Friedman, N F Krebs
BACKGROUND/OBJECTIVES: The impact of maternal BMI and insulin sensitivity on bioactive components of human milk (HM) is not well understood. As the prevalence of obesity and diabetes rises, it is increasingly critical that we understand how maternal BMI and hormones associated with metabolic disease relate to concentrations of bioactive components in HM. SUBJECTS/METHODS: This longitudinal cohort design followed 48 breastfeeding mothers through the first four months of lactation, collecting fasting morning HM samples at 2-weeks and 1, 2, 3 and 4-months, and fasting maternal blood at 2-weeks and 4-months...
May 17, 2017: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28511942/effect-of-resistant-and-digestible-rice-starches-on-human-cytokine-and-lactate-metabolic-networks-in-serum
#17
Huisong Wang, Guangchang Pang
Resistant starch generated after treating ordinary starch is of great significance to human health in the countries with overnutrition. However, its functional evaluation in the human body has been rarely reported. By determining the lactate metabolic flux, 12 serum enzymes expression level and 38 serum cytokines in healthy volunteers, the variation in cytokine network and lactate metabolic network in serum were investigated to compare the mechanism of the physiological effects between the two starches. The results indicated that compared with digestible starch, resistant starch had anti-inflammatory effects, increased anabolism, and decreased catabolism...
May 13, 2017: Cytokine
https://www.readbyqxmd.com/read/28511903/novel-role-of-the-nutraceutical-bioactive-compound-berberine-in-lectin-like-oxldl-receptor-1-mediated-endothelial-dysfunction-in-comparison-to-lovastatin
#18
C Caliceti, P Rizzo, R Ferrari, F Fortini, G Aquila, E Leoncini, L Zambonin, B Rizzo, D Calabria, P Simoni, M Mirasoli, M Guardigli, S Hrelia, A Roda, A F G Cicero
BACKGROUND AND AIMS: Oxidized LDL (oxLDL) or pro-inflammatory stimuli lead to increased oxidative stress linked to endothelial dysfunction and atherosclerosis. The oxLDL receptor-1 (LOX1) is elevated within atheromas and cholesterol-lowering statins inhibit LOX1 expression. Berberine (BBR), an alkaloid extracted from plants of gender Berberis, has lipid-lowering and anti-inflammatory activity. However, its role in regulating LOX1-mediated signaling is still unknown. The aim of this study was to investigate the effect of BBR on oxLDL- and TNFα-induced endothelial dysfunction in human umbilical vein endothelial cells (HUVECs) and to compare it with that of lovastatin (LOVA)...
April 19, 2017: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
https://www.readbyqxmd.com/read/28507419/exploring-the-potential-hazard-of-mycobacterium-avium-subspecies-paratuberculosis-as-a-cause-for-crohn-s-disease
#19
Sukumar Bharathy, Lakshmanasami Gunaseelan, Kannan Porteen
BACKGROUND: Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of Johne's disease (JD), or paratuberculosis in ruminants has been suspected to be implicated in the pathogenesis of Crohn's disease (CD) in humans with chronic inflammatory intestinal changes. As the hypothesis is now fast being recognized that MAP could possibly be the etiological agent of CD which is found to be excreted in milk of dairy animals subclinically or terminally ill with JD. AIM: The present study was aimed to detect MAP in milk by polymerase chain reaction (PCR) targeting IS900 and to describe the excretion pattern of MAP in milk from asymptomatic lactating cows and does with relevance to the public health significance...
April 2017: Veterinary World
https://www.readbyqxmd.com/read/28504908/mammea-longifolia-planch-and-triana-fruit-extract-induces-cell-death-in-the-human-colon-cancer-cell-line-sw480-via-mitochondria-related-apoptosis-and-activation-of-p53
#20
Chunmei Li, Yoonhwa Jeong, Misook Kim
The methanol extract of Mammea longifolia Planch. and Triana (M. longifolia) fruit was studied for anticancer and apoptotic effects in the SW480 colon cancer cell line. The apoptotic and necrotic effects of M. longifolia were detected by 3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H tetrazolium bromide (MTT) and lactate dehydrogenase assays, respectively. One hundred μg/mL of the extract killed ∼82.4% of the cells; however, 2% of the death was related to necrosis. The morphological changes in M. longifolia-stimulated SW480 cells were observed directly by light microscopy...
May 2017: Journal of Medicinal Food
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