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three dimensional

Ai Shima, Yuya Morimoto, H Lee Sweeney, Shoji Takeuchi
This paper describes a method to construct three-dimensional (3D) contractile human skeletal muscle tissues from a cell line. The 3D tissue was fabricated as a fiber-based structure and cultured for two weeks under tension by anchoring its both ends. While myotubes from the immortalized human skeletal myocytes used in this study never contracted in the conventional two-dimensional (2D) monolayer culture, myotubes in the 3D tissue showed spontaneous contraction at a high frequency and also reacted to the electrical stimulation...
June 18, 2018: Experimental Cell Research
Tiecheng Wang, Yang Cao, Guangzhou Qu, Qiuhong Sun, Tianjiao Xia, Xuetao Guo, Hanzhong Jia, Lingyan Zhu
Strong complexation between heavy metals and organic complexing agents makes the heavy metals difficult to be removed by classical chemical precipitation. In this study, a novel decomplexation method was developed using discharge plasma oxidation, which was followed by alkaline precipitation to treat water containing heavy metal-organic complex, i.e., Cu-ethylenediaminetetraacetic acid (Cu-EDTA). The decomplexation efficiency of Cu complex reached up to nearly 100% after 60 min's oxidation by discharge plasma, which was accompanied by 82...
June 21, 2018: Environmental Science & Technology
Yuanyuan Dai, Peng Lv, Ashkan Javadzadegan, Xiao Tang, Yi Qian, Jiang Lin
Background: The carotid blood flow following carotid endarterectomy (CEA) is not fully understood. Computational fluid dynamics (CFD) is a promising method to study blood flow. This study is to investigate local hemodynamic characteristics after CEA via the use of unenhanced magnetic resonance angiography (MRA) and CFD. Methods: Eight carotid arteries with atherosclerosis and sixteen normal carotid arteries were included in this study. Time-of-flight (TOF) and phase contrast (PC) MRA were applied for the measurement of three-dimensional artery geometries and velocity profile under CFD simulation...
May 2018: Quantitative Imaging in Medicine and Surgery
Mark R Bowers, Padraig Gearoid O'Neill, Rohit Bhaskar, Arash Aryana
No abstract text is available yet for this article.
June 2018: HeartRhythm Case Reports
Fumihisa Nakamura, Ichiro Tsukamoto, Shinji Inoue, Kazuhiko Hashimoto, Masao Akagi
Angiotensin II type 1 receptor (AT1R) appears to have a mechanosensing function in a number of cell types. The purpose of this study was to examine whether AT1R expressed in articular chondrocytes is involved in osteoarthritis (OA) progression in vivo and whether cyclic compressive loading activates the AT1R and stimulates hypertrophic differentiation of chondrocytes in vitro . The relationships between the modified Mankin score for cartilage degeneration and the expression of AT1R and type X collagen (Col X) were studied in mouse knees with OA induced using the destabilization-of-medial-meniscus model...
June 2018: FEBS Open Bio
Giovanni Nicoletti, Marco Mario Tresoldi, Alberto Malovini, Marco Visaggio, Angela Faga, Silvia Scevola
Background: Dermal substitutes are currently largely used for the treatment of huge skin loss in patients in critical general health conditions, for the treatment of severe burns and to promote the healing process in chronic wounds. Aims: The authors performed a retrospective assessment of their experience with bioengineered skin to possibly identify the most appropriate clinical indication and management for each substitute. Materials and Methods: The study involved 109 patients with 127 skin defects repaired with dermal substitutes over a 9 years period, from 2007 to 2016...
January 2018: Indian Journal of Plastic Surgery: Official Publication of the Association of Plastic Surgeons of India
Aleksandra Sljivic, Milena Pavlovic Kleut, Zoran Bukumiric, Vera Celic
INTRODUCTION: Echocardiography represents the most commonly performed noninvasive cardiac imaging test for patients with heart failure (HF). The aim of this study was to assess the relationship between exercise capacity parameters (peak oxygen consumption (VO2) and the minute ventilation-carbon dioxide production relationship (VE/VCO2)), two-dimensional speckle-tracking echocardiography (2D-STE) and three-dimensional echocardiography (3DE) imaging of right ventricular (RV) function in HF patients with reduced ejection fraction (EF)...
2018: PloS One
Jeffrey H Lee, Leonard B Kaban, Michael J Yaremchuk
BACKGROUND: Le fort I maxillary osteotomies and sagittal split mandibular osteotomies are performed to correct significant dentofacial deformities. The multidimensional skeletal movements, particularly those of large magnitude may result in contour irregularities and facial imbalances. METHODS: Three dimensional images were reconstructed from computed tomographic (CT) scans in patients unhappy with their appearances after Le Fort I advancement and/or bilateral sagittal split osteotomies (BSSO)...
June 14, 2018: Plastic and Reconstructive Surgery
Min Sun, Guo C Chen, Yong Q Wang, Tao Song, Hai D Li, Di Wu, Ning B Yin
The anatomy of the modiolus and surrounding muscles is complicated and contributes to the morphology of the mouth corner. We used iodine staining and micro-computed tomography (CT) to observe the anatomy of the modiolus and surrounding muscles. Based on the micro-CT images and the 3D model construction, we found that the muscle fibers in the modiolus were oriented continuously in the running direction of the muscle itself and that there were no chaotic clumps in the region of muscle fiber convergence. The pars marginalis of the orbicularis oris muscle connects with the deep buccinators muscle, which may account for how the pars marginalis fibers act to press the upper lip against the maxillary teeth or invert it closer to the oral cavity...
June 14, 2018: Plastic and Reconstructive Surgery
Yue Zhao, Bin Yang
OBJECTIVE: To explore the value of micro surface coil combined with three-dimensional reversed fast imaging with steady-state precession and diffusion-weighted imaging (3D-PSIF-DWI) in displaying intraparotid facial nerves and parotid ducts. METHODS: In total 24 healthy volunteers with no parotid disease underwent scanning of head and neck coil and 4-cm micro surface coil combined with 3D-PSIF-DWI prospectively. The obtained original images were processed through maximum intensity projection, multiplanar reconstruction, and curved planar reconstruction...
June 20, 2018: Journal of Craniofacial Surgery
Xinrui Huang, Sha Li, Song Gao
Cryo-electron microscopy (Cryo-EM) imaging has the unique potential to bridge the gap between cellular and molecular biology by revealing the structures of large macromolecular assemblies and cellular complexes. Therefore, cryo-EM three-dimensional (3D) reconstruction has been rapidly developed in recent several years and applied widely in life science research; however, it suffers from reduced contrast and low signal-to-noise ratios with a high degree of noise under low electron dose conditions, resulting in failures of many conventional filters...
June 21, 2018: Journal of Computational Biology: a Journal of Computational Molecular Cell Biology
Kirby K H Smithe, Chris D English, Saurabh V Suryavanshi, Eric Pop
Two-dimensional semiconductors such as monolayer MoS2 are of interest for future applications including flexible electronics and end-of-roadmap technologies. Most research to date has focused on low-field mobility, but the peak current-driving ability of transistors is limited by the high-field saturation drift velocity, vsat . Here, we measure high-field transport as a function of temperature for the first time in high-quality synthetic monolayer MoS2 . We find that in typical device geometries self-heating can significantly reduce vsat during high-field operation...
June 21, 2018: Nano Letters
Audrey Giocanti-Auregan, Alain Gaudric, Frédérique Buffon, Manuele Mine, Corinne Delahaye-Mazza, Salomon Y Cohen, Ali Erginay, Hugues Chabriat, Elisabeth Tournier Lasserve, Valérie Krivosic
BACKGROUND AND OBJECTIVE: To analyze the location of familial retinal arterial tortuosity (fRAT) in the three-dimensional structure of retinal capillaries. PATIENTS AND METHODS: Retrospective observational study. Twelve eyes of six patients (two of whom were brothers) were imaged by optical coherence tomography angiography (OCTA). The data from their ocular and systemic examinations were recorded. RESULTS: OCTA imaging clearly showed increased tortuosity of second- and third-order retinal arteries in all cases, visible in the superficial vascular plexus (SVP) up to the arteriole termination in the capillaries...
June 1, 2018: Ophthalmic Surgery, Lasers & Imaging Retina
Tatiana E Gorelik, Jacco van de Streek, Herbert Meier, Lars Andernach, Till Opatz
The solid-state structure of star-shaped 2,4,6-tris{(E)-2-[4-(dimethylamino)-phenyl]ethenyl}-1,3,5-triazine is determined from a powder sample by exploiting the respective strengths of single-crystal three-dimensional electron diffraction and powder X-ray diffraction data. The unit-cell parameters were determined from single crystal electron diffraction data. Using this information, the powder X-ray diffraction data were indexed, and the crystal structure was determined from the powder diffraction profile. The compound crystallizes in a noncentrosymmetric space group, P21 21 21 ...
June 1, 2018: Acta Crystallographica Section B, Structural Science, Crystal Engineering and Materials
Ora Nakash, Maayan Nagar, Drew Westen
Prototype matching, which involves comparing a patient clinical presentation with a prototype description of the disorder addresses some of the clinical limitations of the Diagnostic and Statistical Manual of Mental Disorders (DSM) and International Classification of Diseases symptom-count approach. Here, we investigated the validity and clinical utility of three diagnostic systems in predicting patient adaptive functioning using a multimethod multi-informant approach. Specifically, we compared a prototype matching approach based on DSM criteria, an empirically derived prototype matching approach, and DSM symptom count diagnostic systems...
June 21, 2018: Personality Disorders
Tohru Taniguchi, Kie Nakano, Kenji Monde
The chemistry of artificial nucleosides is associated with the difficulties in the characterization of the stereochemistry and conformation of their furanose ring moiety. This unit describes how to use vibrational circular dichroism (VCD) spectroscopy to identify the three-dimensional structure of nucleosides. The experimental part of this protocol is dedicated to obtain a VCD spectrum of a sample with high S/N ratio. The computational part generally starts with a conformational search using molecular mechanics and the following structural optimization by density functional theory calculation...
March 2018: Current Protocols in Nucleic Acid Chemistry
Peter O Bayguinov, Dennis M Oakley, Chien-Cheng Shih, Daniel J Geanon, Matthew S Joens, James A J Fitzpatrick
Since its commercialization in the late 1980's, confocal laser scanning microscopy (CLSM) has since become one of the most prevalent fluorescence microscopy techniques for three-dimensional structural studies of biological cells and tissues. The flexibility of the approach has enabled its application in a diverse array of studies, from the fast imaging of dynamic processes in living cells, to meticulous morphological analyses of tissues, and co-localization of protein expression patterns. In this chapter, we introduce the principles of confocal microscopy and discuss how the approach has become a mainstay in the biological sciences...
June 20, 2018: Current Protocols in Cytometry
Dan C Wilkinson, Michael Mellody, Luisa K Meneses, Ashley C Hope, Bruce Dunn, Brigitte N Gomperts
This unit describes a protocol for generation of lung organoids. A lung organoid is a 3D cell/hydrogel composite that resembles the morphology and cellular composition of the human distal lung. These tissue-engineered constructs provide an in vitro model of human lung and are best suited for disease modeling applications. The organoid generation methodology is flexible, allowing for easy scalability in the number of organoids produced and in the ability to accommodate a wide range of cell types. © 2018 by John Wiley & Sons, Inc...
June 7, 2018: Current Protocols in Stem Cell Biology
Nina M Poole, Anubama Rajan, Anthony W Maresso
Adherence, invasion, and translocation to and through the intestinal epithelium are important drivers of disease for many enteric bacteria. However, most work has been limited to transformed intestinal cell lines or murine models that often do not faithfully recapitulate key elements associated with human disease. The recent technological advances in organotypic tissue and cell culture are providing unparalleled access to systems with human physiology and complexity. Human intestinal enteroids (HIEs), derived from patient biopsy or surgical specimens of intestinal tissues, are organotypic cultures now being adapted to the study of enteric infections...
May 17, 2018: Current Protocols in Microbiology
Arja Ray, Rachel K Morford, Paolo P Provenzano
Cell migration is strongly influenced by the organization of the surrounding 3-D extracellular matrix. In particular, within fibrous solid tumors, carcinoma cell invasion may be directed by patterns of aligned collagen in the extra-epithelial space. Thus, studying the interactions of heterogeneous populations of cancer cells that include the stem/progenitor-like cancer stem cell subpopulation and aligned collagen networks is critical to our understanding of carcinoma dissemination. Here, we describe a robust method to generate aligned collagen matrices in vitro that mimic in vivo fiber organization...
May 24, 2018: Current Protocols in Stem Cell Biology
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