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https://www.readbyqxmd.com/read/28340427/identification-and-characterization-of-a-%C3%AE-defensin-gene-involved-in-the-immune-defense-response-of-channel-catfish-ictalurus-punctatus
#1
Jieyao Zhu, Hong Wang, Jun Wang, Xingli Wang, Shuang Peng, Yi Geng, Kaiyu Wang, Pin Ouyang, Zhiqiong Li, Xiaoli Huang, Defang Chen
Antimicrobial peptides are small peptides that play important roles in a host's innate immune response. As an important antimicrobial peptide, β-defensin widely distribute in mammals, insects and plants with broad-spectrum antimicrobial activity. In this study, the β-defensin gene of the channel catfish, Ictalurus punctatus, was cloned, sequenced, and subjected to a bioinformatic analysis. The β-defensin gene of the channel catfish contains three exons and two introns, and encodes a precursor peptide consisting of two domains: a signal peptide of 24 amino acid residues and a mature peptide of 43 amino acid residues...
March 21, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28340394/assessment-of-engineered-surfaces-roughness-by-high-resolution-3d-sem-photogrammetry
#2
L C Gontard, J D López-Castro, L González-Rovira, J M Vázquez-Martínez, F M Varela-Feria, M Marcos, J J Calvino
We describe a methodology to obtain three-dimensional models of engineered surfaces using scanning electron microscopy and multi-view photogrammetry (3DSEM). For the reconstruction of the 3D models of the surfaces we used freeware available in the cloud. The method was applied to study the surface roughness of metallic samples patterned with parallel grooves by means of laser. The results are compared with measurements obtained using stylus profilometry (PR) and SEM stereo-photogrammetry (SP). The application of 3DSEM is more time demanding than PR or SP, but it provides a more accurate representation of the surfaces...
March 7, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28340387/an-exploration-of-the-effect-and-interaction-mechanism-of-bisphenol-a-on-waste-sludge-hydrolysis-with-multi-spectra-isothermal-titration-microcalorimetry-and-molecule-docking
#3
Guangying Hou, Rui Zhang, Xiaoyan Hao, Chunguang Liu
An increasing amount of bisphenol A (BPA) is being produced and used, then discharged into sewage treatment plants and accumulated in sludge or soil, when the sludge is used as fertilizer. Accumulation of BPA in sludge or soil causes poisoning to the enzyme, which affects the biological treatment of sludge and the circulation and conversion of materials in soil. In this research, effect of BPA on sludge hydrolysis is studied from the respect of concentration and components of soluble organic matter in sludge, using three-dimensional fluorescence spectra...
March 10, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28340329/a-close-up-view-of-codon-selection-in-eukaryotic-initiation
#4
Christoffer Lind, Mauricio Esguerra, Johan Åqvist
When given an option to choose among a set of alternatives and only one selection is right, one might stop and reflect over which one is best. However, the ribosome has no time to stop and make such reflections, proteins need to be produced and very fast. Eukaryotic translation initiation is an example of such a conundrum. Here, scanning for the correct codon match must be fast, efficient and accurate. We highlight our recent computational findings, which show how the initiation machinery manages to recognize one specific codon among many possible challengers, by fine-tuning the energetic landscape of base-pairing with the aid of the initiation factors eIF1 and eIF1A...
March 24, 2017: RNA Biology
https://www.readbyqxmd.com/read/28340110/peri-mitral-atrial-flutter-personalized-ablation-strategy-based-on-arrhythmogenic-substrate
#5
Jinbo Yu, Kai Chen, Bing Yang, Fengxiang Zhang, Weizhu Ju, Hongwu Chen, Gang Yang, Mingfang Li, Benqi Wang, Kai Gu, Feifan Ouyang, Siew Yen Ho, Sunny Po, Minglong Chen
Aims: The aim of this study is to characterize the arrhythmogenic substrate for peri-mitral atrial flutter (PMAFL), thereby determining a personalized ablation strategy to treat PMAFL. Methods and results: Thirty-six consecutive PMAFL patients (mean age: 63.8 ± 11.3, 23 males) underwent detailed three-dimensional electroanatomic mapping in left atrium (LA). The LA was divided into septal-anterior wall (SAW), posterior inferior wall (PIW), and mitral isthmus (MI) region, respectively...
March 1, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28340078/image-integration-into-3-dimensional-electro-anatomical-mapping-system-facilitates-safe-ablation-of-ventricular-arrhythmias-originating-from-the-aortic-root-and-its-vicinity
#6
Mario Jularic, Ruken Özge Akbulak, Benjamin Schäffer, Julia Moser, Jana Nuehrich, Christian Meyer, Christian Eickholt, Stephan Willems, Boris A Hoffmann
Aims: During ablation in the vicinity of the coronary arteries establishing a safe distance from the catheter tip to the relevant vessels is mandatory and usually assessed by fluoroscopy alone. The aim of the study was to investigate the feasibility of an image integration module (IIM) for continuous monitoring of the distance of the ablation catheter tip to the main coronary arteries during ablation of ventricular arrhythmias (VA) originating in the sinus of valsalva (SOV) and the left ventricular summit part of which can be reached via the great cardiac vein (GCV)...
March 7, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28339918/can-clinically-relevant-dose-errors-in-patient-anatomy-be-detected-by-gamma-passing-rate-or-modulation-complexity-score-in-volumetric-modulated-arc-therapy-for-intracranial-tumors
#7
Shingo Ohira, Yoshihiro Ueda, Masaru Isono, Akira Masaoka, Misaki Hashimoto, Masayoshi Miyazaki, Masaaki Takashina, Masahiko Koizumi, Teruki Teshima
We investigated whether methods conventionally used to evaluate patient-specific QA in volumetric-modulated arc therapy (VMAT) for intracranial tumors detect clinically relevant dosimetric errors. VMAT plans with coplanar arcs were designed for 37 intracranial tumors. Dosimetric accuracy was validated by using a 3D array detector. Dose deviations between the measured and planned doses were evaluated by gamma analysis. In addition, modulation complexity score for VMAT (MCSv) for each plan was calculated. Three-dimensional dose distributions in patient anatomy were reconstructed using 3DVH software, and clinical deviations in dosimetric parameters between the 3DVH doses and planned doses were calculated...
March 3, 2017: Journal of Radiation Research
https://www.readbyqxmd.com/read/28339815/relationships-between-standing-frontal-plane-knee-alignment-and-dynamic-knee-joint-loading-during-walking-and-jogging-in-youth-who-are-obese
#8
Matthew S Briggs, Sharon Bout-Tabaku, Michael P McNally, Ajit M W Chaudhari, Thomas M Best, Laura C Schmitt
Background: Youth who are obese have high risk of poor knee health and cartilage damage. Understanding factors which may affect knee health in youth who are obese is critical for preservation of knee integrity and function. Objective: Compare standing frontal plane knee alignment and knee loading patterns between youth who are obese and healthy-weight and determine the association between knee alignment and knee loading patterns during walking and jogging. Design: Cross sectional matched-pair...
February 28, 2017: Physical Therapy
https://www.readbyqxmd.com/read/28339648/three-dimensional-testicular-organoid-a-novel-tool-for-the-study-of-human-spermatogenesis-and-gonadotoxicity-in-vitro%C3%A2
#9
Samuel S Pendergraft, Hooman Sadri-Ardekani, Anthony Atala, Colin E Bishop
Existing methods for evaluating the potential gonadotoxicity of environmental agents and pharmaceutical compounds rely heavily on animal studies. The current gold standard in vivo functional assays in animals are limited in their human predictive capacity. In addition, existing human two-dimensional in vitro models of testicular toxicity do not accurately reflect the in vivo situation. A more reliable testicular in vitro model system is needed to better assess the gonadotoxic potential of drugs prior to progression into clinical trials...
February 17, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28339627/validity-and-reliability-of-three-dimensional-palatal-superimposition-of-digital-dental-models
#10
Sameh Talaat, Ahmed Kaboudan, Christoph Bourauel, Nivine Ragy, Katherine Kula, Ahmed Ghoneima
No abstract text is available yet for this article.
February 17, 2017: European Journal of Orthodontics
https://www.readbyqxmd.com/read/28339603/optimization-of-stem-imaging-conditions-for-cryo-tomography
#11
Kazuhiro Aoyama, Kotaro Nagano, Kaoru Mitsuoka
For three-dimensional analysis of cryo-specimens using tomography at low and medium magnifications, the use of STEM images has some important advantages over TEM. A clear understanding of the electron dose in STEM is essential for optimized cryo-microscopy so a dose estimation technique for STEM imaging has been developed. The STEM convergence angle was shown to have a large impact on the image quality, in particular on the signal-to-noise ratio in the acquired images. Importantly, there is a clear trade-off between good STEM spatial resolution in the images with a large convergence angle and the signal-to-noise ratio, which is improved by reducing the convergence angle as much as possible...
February 9, 2017: Microscopy
https://www.readbyqxmd.com/read/28339542/cervical-epidural-contrast-spread-patterns-in-fluoroscopic-antero-posterior-lateral-and-contralateral-oblique-view-a-three-dimensional-analysis
#12
Jatinder Gill, Jyotsna Nagda, Moris Aner, Thomas Simopoulos
Objective.:  To describe and to analyze cervical epidural contrast patterns seen in antero-posterior (AP), contralateral oblique (CLO), and lateral view. To identify factors that might help in predicting contrast distribution pattern and extent. Method.:  Spread of contrast in the cervical epidural space was prospectively studied in AP, lateral, and three CLO views. Results.:  CLO view showed contrast spread of variable thickness with its posterior margin overlying the ventral interlaminar line (VILL)...
October 7, 2016: Pain Medicine: the Official Journal of the American Academy of Pain Medicine
https://www.readbyqxmd.com/read/28339423/spherically-symmetric-volume-elements-as-basis-functions-for-image-reconstructions-in-computed-laminography
#13
Patrick Trampert, Jonas Vogelgesang, Christian Schorr, Michael Maisl, Sviatoslav Bogachev, Nico Marniok, Alfred Louis, Tim Dahmen, Philipp Slusallek
BACKGROUND: Laminography is a tomographic technique that allows three-dimensional imaging of flat and elongated objects that stretch beyond the extent of a reconstruction volume. Laminography images can be reconstructed using iterative algorithms based on the Kaczmarz method. OBJECTIVE: This study aims to develop and demonstrate a new reconstruction algorithm that may provide superior image reconstruction quality for this challenged imaging application. METHODS: The images are initially represented using the coefficients over basis functions, which are typically piecewise constant functions (voxels)...
March 21, 2017: Journal of X-ray Science and Technology
https://www.readbyqxmd.com/read/28339190/luminescent-zn-ii-coordination-polymers-for-highly-selective-sensing-of-cr-iii-and-cr-vi-in-water
#14
Tian-Yi Gu, Ming Dai, David James Young, Zhi-Gang Ren, Jian-Ping Lang
Three photoluminescent zinc coordination polymers (CPs), {[Zn2(tpeb)2(2,5-tdc)(2,5-Htdc)2]·2H2O}n (1), {[Zn2(tpeb)2(1,4-ndc)(1,4-Hndc)2]·2.6H2O}n (2), and {[Zn2(tpeb)2(2,3-ndc)2]·H2O}n (3) (tpeb = 1,3,5-tri-4-pyridyl-1,2-ethenylbenzene, 2,5-tdc = 2,5-thiophenedicarboxylic acid, 1,4-ndc = 1,4-naphthalenedicarboxylic acid, and 2,3-ndc = 2,3-naphthalenedicarboxylic acid) were prepared from reactions of Zn(NO3)2·6H2O with tpeb and 2,5-H2tdc, 1,4-H2ndc, or 2,3-H2ndc under solvothermal conditions. Compound 1 has a two-dimensional (2D) grid-like network formed from bridging 1D [Zn(tpeb)]n chains via 2,5-tdc dianions...
March 24, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28339117/intraexaminer-and-interexaminer-variability-in-3d-fetal-volume-measurements-during-the-second-and-third-trimesters-of-pregnancy
#15
Louise E Simcox, Lucy E Higgins, Jenny E Myers, Edward D Johnstone
OBJECTIVES: To assess intraexaminer and interexaminer reliability of 3-dimensional fetal sonographic measurements. METHODS: Three-dimensional fetal organ volumes (head, kidney, total thigh volume, and fractional thigh volume) were acquired during the second and third trimesters, with the addition of placental volume in the second trimester, by 2 different experienced, blinded sonographers. Fifty-eight fetuses were examined from 21 to 39 weeks' gestation. Intraexaminer and Interexaminer reliability was assessed with Bland-Altman plots, and their 95% limits of agreement and intraclass correlation coefficients...
March 24, 2017: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/28338854/comparison-of-the-peripheral-doses-from-different-imrt-techniques-for-pediatric-head-and-neck-radiation-therapy
#16
Masahiko Toyota, Yasumasa Saigo, Kenta Higuchi, Takuya Fujimura, Chihaya Koriyama, Takashi Yoshiura, Suminori Akiba
Intensity-modulated radiation therapy (IMRT) can deliver high and homogeneous doses to the target area while limiting doses to organs at risk. We used a pediatric phantom to simulate the treatment of a head and neck tumor in a child. The peripheral doses were examined for three different IMRT techniques [dynamic multileaf collimator (DMLC), segmental multileaf collimator (SMLC) and volumetric modulated arc therapy (VMAT)]. Peripheral doses were evaluated taking thyroid, breast, ovary and testis as the points of interest...
February 25, 2017: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/28338831/improved-clearing-of-lipid-droplet-rich-tissues-for-three-dimensional-structural-elucidation
#17
Mengjie Lai, Xiaowei Li, Jun Li, Yan Hu, Daniel M Czajkowsky, Zhifeng Shao
No abstract text is available yet for this article.
March 10, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28338536/dual-energy-computed-tomography-for-the-evaluation-of-enhancement-of-pulmonary-nodules%C3%A2-3-cm-in-size
#18
Michael J Reiter, Weston T Winkler, Kenton E Kagy, Ryan B Schwope, Christopher J Lisanti
PURPOSE: The aim of the study was to compare the accuracies of 4 different methods of assessing pulmonary nodule enhancement to distinguish benign from malignant solid pulmonary nodules using nondynamic contrast-enhanced dual-energy computed tomography. MATERIALS AND METHODS: Seventy-two patients (mean age, 62 y) underwent dual-energy chest computed tomography 3 minutes after intravenous contrast administration. Each of 118 pulmonary nodules (9±5.9 mm) were evaluated for enhancement by 4 methods: visual assessment, 3-dimensional automated postprocessing measurement tool, manually drawn region of interest with calculated iodine-related attenuation, and measurement of iodine concentration...
March 22, 2017: Journal of Thoracic Imaging
https://www.readbyqxmd.com/read/28338443/fluorescent-labeling-of-collagen-production-by-cells-for-noninvasive-imaging-of-extracellular-matrix-deposition
#19
Katie Bardsley, Ying Yang, Alicia J El Haj
Extracellular matrix (ECM) is an essential component of tissues and provides both integrity and biological cues for cells. Collagen is one of the major proteins found within the ECM and therefore is an essential component of all engineered tissues. Therefore, in this article, we present a method for the online real-time monitoring of collagen deposition in three-dimensional engineered constructs. This method revolves around modification of collagen through the addition of azide-L-proline to cell culture media...
March 24, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28338421/harnessing-the-osteogenicity-of-in-vitro-stem-cell-derived-mineralized-extracellular-matrix-as-3d-biotemplate-to-guide-bone-regeneration
#20
Yoke Chin Chai, Johanna Bolander, Ioannis Papantoniou, Jennifer Patterson, Jef Vleugels, Jan Schrooten, Frank P Luyten
Advanced biomaterials that are capable of guiding robust bone regeneration are highly demanded for translational therapy of bone defects or bone augmentation in clinics. One of the strategic approaches is to produce tissue engineering (TE) constructs that mediate bone regeneration by recapitulating the natural bone formation or healing process. In this study, we aimed at producing devitalized mineralized carriers with augmented bone forming capacity via a modified culture protocol (i.e., culture conditions with high calcium and/or phosphate concentrations) that first promotes cell growth and, subsequently, mineralized extracellular matrix (ECM) deposition by human periosteum-derived osteoprogenitor cells (hPDCs) on additive manufactured three-dimensional (3D) porous titanium (Ti)-based scaffolds...
March 24, 2017: Tissue Engineering. Part A
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