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https://www.readbyqxmd.com/read/27911364/an-intramedullary-locking-nail-for-standardized-fixation-of-femur-osteotomies-to-analyze-normal-and-defective-bone-healing-in-mice
#1
Tina Histing, Michael D Menger, Tim Pohlemann, Romano Matthys, Tobias Fritz, Patric Garcia, Moritz Klein
Bone healing models are essential to the development of new therapeutic strategies for clinical fracture treatment. Furthermore, mouse models are becoming more commonly used in trauma research. They offer a large number of mutant strains and antibodies for the analysis of the molecular mechanisms behind the highly differentiated process of bone healing. To control the biomechanical environment, standardized and well-characterized osteosynthesis techniques are mandatory in mice. Here, we report on the design and use of an intramedullary nail to stabilize open femur osteotomies in mice...
November 13, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911273/robust-detection-of-immune-transcripts-in-ffpe-samples-using-targeted-rna-sequencing
#2
Benjamin E Paluch, Sean T Glenn, Jeffrey M Conroy, Antonios Papanicolau-Sengos, Wiam Bshara, Angela R Omilian, Elizabeth Brese, Mary Nesline, Blake Burgher, Jonathan Andreas, Kunle Odunsi, Kevin Eng, Ji He, Maochun Qin, Mark Gardner, Lorenzo Galluzzi, Carl D Morrison
Current criteria for identifying cancer patients suitable for immunotherapy with immune checkpoint blockers (ICBs) are subjective and prone to misinterpretation, as they mainly rely on the visual assessment of CD274 (best known as PD-L1) expression levels by immunohistochemistry (IHC). To address this issue, we developed a RNA sequencing (RNAseq)-based approach that specifically measures the abundance of immune transcripts in formalin-fixed paraffin embedded (FFPE) specimens. Besides exhibiting superior sensitivity as compared to whole transcriptome RNAseq, our assay requires little starting material, implying that it is compatible with RNA degradation normally caused by formalin...
November 29, 2016: Oncotarget
https://www.readbyqxmd.com/read/27911229/c-2-arteriovenous-fistula-presenting-as-a-pathologic-hangman-s-fracture-case-report
#3
Rajeev D Sen, Carolina Gesteira Benjamin, Howard A Riina, Donato Pacione
The authors report on an 81-year-old woman with a pathologic hangman's fracture secondary to a complex arteriovenous fistula (AVF). The patient presented with severe, unremitting neck pain and was found to have fractures bilaterally through the pars interarticularis of C-2 with significant anterior subluxation of C-2 over C-3 along with widening of the left transverse foramen. Due to an abnormally appearing left vertebral artery (VA) on CT angiography, the patient underwent conventional angiography, which revealed a complex AVF stemming from the left VA at the level of C-2 with dilated posterior cervical veins and a large venous varix...
December 2, 2016: Journal of Neurosurgery. Spine
https://www.readbyqxmd.com/read/27910709/management-of-infections-associated-with-neurosurgical-implanted-devices
#4
Anna Conen, Christoph A Fux, Peter Vajkoczy, Andrej Trampuz
Neurosurgical devices are increasingly used. With it, neurosurgical device-related infections gain relevance. As biofilms are involved in implant-associated infections the diagnosis and treatment is challenging and requires specific anti-biofilm concepts and management algorithms. Areas covered: The literature concerning the management of neurosurgical device-associated infections is scarce and heterogeneous treatment concepts are discussed, but no standardized diagnostic and treatment procedures exist. Therefore, we emphasize extrapolating management strategies predominantly from orthopedic device-associated infections, where the concept is better established and clinically validated...
December 2, 2016: Expert Review of Anti-infective Therapy
https://www.readbyqxmd.com/read/27910010/chromosome-preparation-for-myeloid-malignancies
#5
Eleanor K C Hui, Thomas S K Wan, Margaret H L Ng
Many cases of myeloid malignancies are associated with recurring cytogenetic aberrations. Chromosomal analysis can aid in diagnosis, predict prognosis, and disclose subsequent clonal evolution. Three different cell culture methods: direct harvest, nonsynchronized culture, and synchronized culture are usually prepared if the nucleated cell counts in marrow blood are sufficient. Synchronized culture is the first choice of method in myeloid malignancies, whereas the direct method can be omitted if the cell count is low...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27909969/subjective-ulnar-nerve-dysfunction-commonly-following-open-reduction-internal-fixation-orif-of-distal-humeral-fractures-and-in-situ-decompression-of-the-ulnar-nerve
#6
Birgitta Svernlöv, Jens Nestorson, Lars Adolfsson
The aim of this retrospective study was to investigate the frequency of persistent ulnar affection in patients who underwent open reduction and internal fixation (ORIF) of distal humeral fractures without ulnar nerve transposition or mobilisation. Eighty-two patients (53 women), mean age 62 years, were, at a mean of 48 months, reviewed through medical records and a subjective evaluation form concerning ulnar nerve problems. Ulnar nerve affliction, in most cases regarded as mild, was experienced by 22 patients (27%; 14 women) and significantly associated with multiple surgeries...
December 1, 2016: Strategies in Trauma and Limb Reconstruction
https://www.readbyqxmd.com/read/27909827/remediation-of-blowouts-by-clonal-plants-in-maqu-degraded-alpine-grasslands-of-northwest-china
#7
JianJun Kang, WenZhi Zhao, Ming Zhao
The sand-fixation of plants is considered to be the most effective and fundamental measure in desertification control in many arid and semi-arid regions. Carex brunnescens (Carex spp) and Leymus secalinus (Leymus), two perennial clonal herbs native to the Maqu degraded alpine areas of northwest China, are dominant and constructive species in active sand dunes that have excellent adaptability to fix sand dunes found to date. In order to study the ability and mechanism of sandland blowout remediation by two clone plants C...
December 1, 2016: Journal of Plant Research
https://www.readbyqxmd.com/read/27909754/dual-plate-fixation-on-distal-third-diaphyseal-fracture-of-the-humerus
#8
Jun-Ku Lee, Yun-Sung Choi, Young-Suk Sim, Dae-Sung Choi, Soo-Hong Han
INTRODUCTION: Displaced unstable distal third fractures of the humeral diaphysis are treated surgically through open reduction and internal fixation. Conventionally, surgeons prefer using long plates for secure fixation; however, we performed short plate dual plating for robust fixation that required a smaller incision and less dissection through an anterior approach. In this study, we report the results of dual plating of fractures of the humeral shaft, with radiographic evidence and clinical analysis...
December 1, 2016: International Orthopaedics
https://www.readbyqxmd.com/read/27909749/diversity-of-total-bacterial-communities-and-chemoautotrophic-populations-in-sulfur-rich-sediments-of-shallow-water-hydrothermal-vents-off-kueishan-island-taiwan
#9
Li Wang, Man Kit Cheung, Rulong Liu, Chong Kim Wong, Hoi Shan Kwan, Jiang-Shiou Hwang
Shallow-water hydrothermal vents (HTVs) are an ecologically important habitat with a geographic origin similar to that of deep-sea HTVs. Studies on shallow-water HTVs have not only facilitated understanding of the influences of vents on local ecosystems but also helped to extend the knowledge on deep-sea vents. In this study, the diversity of bacterial communities in the sediments of shallow-water HTVs off Kueishan Island, Taiwan, was investigated by examining the 16S ribosomal RNA gene as well as key functional genes involved in chemoautotrophic carbon fixation (aclB, cbbL and cbbM)...
December 1, 2016: Microbial Ecology
https://www.readbyqxmd.com/read/27909678/bridge-tenodesis-a-secure-fixation-technique-for-biceps-long-head-tendinopathy-during-arthroscopic-rotator-cuff-repair-using-a-suture-bridge-technique
#10
Jin-Young Park, Jae-Hyung Lee, Kyung-Soo Oh, Seok-Won Chung, Jin-Young Bang, Young-Min Noh
Tendinopathy of the long head of the biceps is often found as an intra-articular pathology in the glenohumeral joint. Because long head of the biceps lesions are common, surgical intervention to properly manage the long head of the biceps has become an important issue. Both tenodesis and tenotomy have been shown to provide benefits in biceps long head tendinopathy. But because of concerns about muscle power reduction, cramping, and "Popeye's deformity," which may result from biceps tenotomy, biceps tenodesis is a good option for treating biceps lesions...
October 2016: Arthroscopy Techniques
https://www.readbyqxmd.com/read/27909668/triple-row-modification-of-the-suture-bridge-technique-for-arthroscopic-rotator-cuff-repair
#11
Roger V Ostrander, Jarrod Smith, Michael Saper
Recent advances to improve outcomes in rotator cuff repair include using arthroscopic double-row suture-bridge techniques in an effort to reconstruct the rotator cuff footprint and improve fixation. However, when using this technique for larger tears, it can be difficult to get the lateral portion of the rotator cuff into an anatomic position. This report describes a triple-row modification of the suture-bridge technique that results in significantly more footprint contact area and contact pressure compared with the double-row and standard suture-bridge techniques...
October 2016: Arthroscopy Techniques
https://www.readbyqxmd.com/read/27909666/arthroscopic-reduction-and-internal-fixation-for-pipkin-type-i-femoral-head-fracture-technical-note
#12
Aditya Kekatpure, Taesoo Ahn, Soong Joon Lee, Mi Youn Jeong, Jae Suk Chang, Pil Whan Yoon
Femoral head fractures after posterior dislocation of the hip are uncommon and are conventionally treated by an open method. Hip arthroscopy can be a valuable treatment option for the management of femoral head fractures. Arthroscopy allows for a less invasive option when compared with arthrotomy and can allow faster recovery with minimal soft-tissue injury. We describe the arthroscopic reduction and internal fixation of Pipkin type I femoral head fractures with a detailed stepwise description of the surgical technique, including technical pearls and pitfalls, potential complications, and advantages and disadvantages...
October 2016: Arthroscopy Techniques
https://www.readbyqxmd.com/read/27909661/the-oblique-mattress-lasso-loop-stitch-for-arthroscopic-capsulolabral-repair
#13
Nata Parnes, Maryellen Blevins, Monica Morman, Paul Carey
Arthroscopic capsulolabral repair during shoulder stabilization surgery requires the use of suture anchors. Several arthroscopic suturing techniques for capsulolabral repair have been described, and each carries very specific advantages and disadvantages with regard to risk, patient satisfaction, and functional outcomes. The purpose of this report is to describe the oblique mattress lasso-loop stitch. This stitch (1) provides strong initial fixation of the labrum, (2) establishes labral height and allows for larger capsular plication if needed, (3) prevents the suture from cutting through the radial fibers of the glenoid labrum, (4) prevents knot migration to the articular side and loosening of the knot, and (5) requires fewer implants and preserves glenoid bone stock by increasing the amount of labrum and capsule that can be reattached to the glenoid with a single-loaded suture anchor...
October 2016: Arthroscopy Techniques
https://www.readbyqxmd.com/read/27909659/reliability-of-the-planned-pedicle-screw-trajectory-versus-the-actual-pedicle-screw-trajectory-using-intra-operative-3d-ct-and-image-guidance
#14
Catherine A Miller, Charles G Ledonio, Matthew A Hunt, Farhan Siddiq, David W Polly
BACKGROUND: Technological advances, including navigation, have been made to improve safety and accuracy of pedicle screw fixation. We evaluated the accuracy of the virtual screw placement (Stealth projection) compared to actual screw placement (intra-operative O-Arm) and examined for differences based on the distance from the reference frame. METHODS: A retrospective evaluation of prospectively collected data was conducted from January 2013 to September 2013. We evaluated thoracic and lumbosacral pedicle screws placed using intraoperative O-arm and Stealth navigation by obtaining virtual screw projections and intraoperative O-arm images after screw placement...
2016: International Journal of Spine Surgery
https://www.readbyqxmd.com/read/27909651/hybrid-decompression-and-fixation-technique-for-the-treatment-of-multisegmental-cervical-spondylotic-myelopathy
#15
Barón Zárate-Kalfopulos, Walter Araos-Silva, Alejandro Reyes-Sánchez, Luis Miguel Rosales-Olivarez, Armando Alpizar-Aguirre, Francisco Lopez Melendez
BACKGROUND: Cervical spondilotic myelopathy (CSM) is defined as the compromise of the spinal cord due to degenerative changes of the cervical spine. It is the most common cause of spinal cord dysfunction in patients over 55 years. An early surgical management it is paramount to achieve better neurological outcome. There is still controversy regarding the appropriate surgical treatment for multisegmental CSM involving three or more levels. The hybrid decompression and fixation technique combines one or two level corpectomy and a single level discectomy in order to obtain optimum decompression and fixation in patients with multilevel cervical myelopathy...
2016: International Journal of Spine Surgery
https://www.readbyqxmd.com/read/27909444/smallholder-farms-and-the-potential-for-sustainable-intensification
#16
Leah M Mungai, Sieglinde Snapp, Joseph P Messina, Regis Chikowo, Alex Smith, Erin Anders, Robert B Richardson, Guiying Li
The sustainable intensification of African agriculture is gaining momentum with the compelling need to increase food and agricultural production. In Southern Africa, smallholder farming systems are predominately maize-based and subject to erratic climatic conditions. Farmer crop and soil management decisions are influenced by a plethora of complex factors such as market access resource availability, social relations, environment, and various messages on sustainable farming practices. Such factors pose barriers to increasing sustainable intensification in Africa...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27909213/hammertoe-correction-with-k-wire-fixation-compared-with-percutaneous-correction
#17
Mustafa Yassin, Avraham Garti, Eyal Heller, Dror Robinson
BACKGROUND: Kirschner wire (K-wire) fixation for correction of hammertoe deformity is the gold standard for hammertoe surgery fixation, the current study compares it to percutaneous surgery with 3M Coban dressings. METHODS: All hammertoe corrections performed were retrospectively reviewed. For the K-wire fixation group: resection arthroplasty of the proximal interphalangeal joint was performed and fixed with a K-wire. The percutaneous technique used involved percutaneous diaphyseal osteotomy of the middle and proximal phalanges combined with tendon release...
November 30, 2016: Foot & Ankle Specialist
https://www.readbyqxmd.com/read/27908804/custom-made-conical-endograft-in-the-treatment-of-saccular-abdominal-aortic-aneurysms-with-tight-and-calcified-distal-neck-thinking-out-of-the-box
#18
Efstratios Georgakarakos, Christos Argyriou, Nikolaos Schoretsanitis, George S Georgiadis
PURPOSE: to describe the use of the combination of a conical custom-made TREO® (TREO CM) stent-graft in the treatment of a saccular abdominal aortic endograft (AAA) with long but tight and calcified distal neck. TECHNIQUE: A 65-year female patient was treated for a saccular 5.2 cm AAA with a 3cm-long but calcified and tight (16mm) distal neck, precluding the safe use of a bifurcated endograft. Since the patient refused an open surgery, a conical TREO CM endograft was manufactured with 20% proximal oversizing, whereas the 3cm caudal sealing segment demonstrated a conical configuration comprised of a 2cm and 1cm nitinol-supported zones of 20% and 10% oversizing, respectively, in order to avoid excessive strain and incomplete expand at the most distal calcified area, leading ultimately to an insidious infolding and consequent type Ib endoleak...
November 28, 2016: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/27908742/arthroscopic-arthrodesis-of-the-shoulder-in-brachial-plexus-palsy
#19
Hubert Lenoir, Thomas Williams, Aude Griffart, Cyril Lazerges, Michel Chammas, Bertrand Coulet, Dominique Le Nen
BACKGROUND: Shoulder arthroscopic arthrodesis is an uncommon and non-standardized procedure. Results are poorly evaluated. We describe the findings in 8 patients who underwent shoulder arthroscopic arthrodesis for brachial plexus injury. METHODS: The humeral head and glenoid were excised through standard arthroscopic portals. A Hoffmann external fixator and cannulated screws were used to stabilize the shoulder. Standard clinical assessment and radiographic evaluation were performed each month until bony fusion occurred...
November 28, 2016: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/27908615/unilaterally-posterior-lumbar-interbody-fusion-with-double-expandable-peek-cages-without-pedicle-screw-support-for-lumbar-disc-herniation
#20
Aydemir Kale, Ibrahim Ilker Oz, Ayhan Onk, Murat Kalaycı, Çağatay Büyükuysal
OBJECTIVES: Posterior lumbar interbody fusion (PLIF) is usually bilateral procedure, and it is combined with posterior by bilateral pedicle screw support or with fixation. The purpose of this retrospective study was to compare the surgical outcomes of simple discectomy and PLIF without pedicle screw support in patients with lumbar disc herniation (LDH). PATIENTS AND METHODS: 60 patients with single segment LDH were operated between February 2010 and June 2013. 40 patients were treated with simple discectomy (Group 1) and 20 patients were treated with PLIF using double expandable polyetheretherketone (PEEK) cages without instrumentation (Group 2) unilaterally...
November 17, 2016: Neurologia i Neurochirurgia Polska
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