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https://www.readbyqxmd.com/read/29236355/b-cell-specific-protein-fcrla-is-expressed-by-plasmacytoid-dendritic-cells-in-humans
#1
Evdokiya Reshetnikova, Sergey Guselnikov, Olga Volkova, Konstantin Baranov, Alexander Taranin, Ludmila Mechetina
BACKGROUND: Fc receptor-like A (FCRLA) is a unique member of a family of Fc receptor like-molecules that lacks a transmembrane region and is an ER-resident protein. In mice and humans, FCRLA has been known as a B cell specific protein. We report here that, in humans, FCRLA is also expressed in a subpopulation of plasmacytoid dendritic cells (pDCs). METHODS: Human peripheral blood mononuclear cells (PBMC), splenocytes, and tonsillar cells were stained for lineage markers followed by fixation/saponin permeabilization and intracellular staining for FCRLA, and then analyzed by flow cytometry with CD123 and CD303 used as pDC markers...
December 13, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29235975/preparing-thin-cross-sections-of-arabidopsis-roots-without-embedding
#2
Naoyuki Sotta, Toru Fujiwara
Here, we describe a method for obtaining thin cross sections of Arabidopsis thaliana roots without fixation and embedding. Roots were grown in pinholes made in a solidified growth medium, and cross sections were prepared without pretreatment. Using this method, we detected unique distributions of two polar-localized proteins-green fluorescent protein (GFP)-tagged BOR1 and NIP5;1-with less sample preparation time than conventional methods. This method is simple, rapid, and yields high-quality cross-section images that are free from artifacts commonly associated with embedding or the sample preparation procedures used in many conventional methods...
December 1, 2017: BioTechniques
https://www.readbyqxmd.com/read/29235974/processing-fixed-and-stored-adipose-derived-stem-cells-for-quantitative-protein-array-assays
#3
Jessica S Sadick, Eric M Darling
Accurately characterizing cellular subpopulations is essential for elucidating the mechanisms underlying normal and pathological biology. Isolation of specific cell types can be accomplished by labeling unique cell-associated proteins with fluorescent antibodies. Cell fixation is commonly used to prepare these samples and allow for long-term storage, but this poses challenges for subsequent protein analysis. We previously established the FITSAR (formaldehyde-fixed intracellular target-sorted antigen retrieval) method, in which protein can be isolated and characterized from fixed, enriched cell subpopulations...
December 1, 2017: BioTechniques
https://www.readbyqxmd.com/read/29234883/accuracy-of-thoracic-pedicle-screw-placement-in-adolescent-patients-with-severe-spinal-deformities-a-retrospective-study-comparing-drill-guide-template-with-free-hand-technique
#4
Yue Pan, G H Lü, Lei Kuang, Bing Wang
PURPOSE: Patients with severe spinal deformities often have small pedicle diameters, and pedicle dimensions vary between segments and individuals. Free-hand pedicle screw placement can be inaccurate. Individualized drill guide templates may be used, but the accuracy of pedicle screw placement in severe scoliosis remains unknown. The accuracy of drill guide templates and free-hand technique for the treatment of adolescent patients with severe idiopathic scoliosis are compared in this study...
December 12, 2017: European Spine Journal
https://www.readbyqxmd.com/read/29234650/experience-in-fixation-of-infected-non-union-tibia-by-ilizarov-technique-a-retrospective-study-of-42-cases
#5
Jimmy Joseph Meleppuram, Syed Ibrahim
Objective: The aim of the study is to evaluate the clinical and functional outcome in treatment of infected tibial nonunion by the Ilizarov method. Methods: The authors retrospectively analyzed 42 patients with infected tibial nonunion with bone loss; shortening and deformity treated at this institution during the period of February 2012 to April 2015 were included in the study. The results were evaluated according to Association for the Study and Application of the Methods of Ilizarov (ASAMI) criteria...
November 2017: Revista Brasileira de Ortopedia
https://www.readbyqxmd.com/read/29234648/supination-external-rotation-ankle-fractures-analysis-of-clinical-results-after-syndesmotic-screw-removal
#6
João Mendonça de Lima Heck, Rosalino Guareschi Junior, Luiz Carlos Almeida da Silva, Marcelo Teodoro Ezequiel Guerra
Objective: To evaluate the postoperative results of patients with supination-external rotation ankle fractures who underwent syndesmotic screw (SS) removal. Methods: Retrospective cohort study assessing the late postoperative results of 35 patients operated from January 2013 to June 2015. Patients undergoing treatment of rupture of the distal tibiofibular syndesmosis with SS fixation and who did not have any concomitant surgical injuries in sites other than the ankle were included...
November 2017: Revista Brasileira de Ortopedia
https://www.readbyqxmd.com/read/29234610/a-new-root-strengthening-technique-for-acute-aortic-dissection-with-a-weakened-aortic-root-the-neo-adventitia-technique
#7
Ji Yong Kim, In Ha Kim, Woon Heo, Ho-Ki Min, Do Kyun Kang, Youn-Ho Hwang, Hee Jae Jun
Background: Dissection flaps in acute type A aortic dissection typically extend into the root, most frequently into the non-coronary sinus (NCS). The weakened root can be susceptible not only to surgical trauma, but also to future dilatation because of its thinner layers. Herein, we describe a new technique that we named the "neo-adventitia" technique to strengthen the weakened aortic root. Methods: From 2012 to 2016, 27 patients with acute type A aortic dissection underwent supracommissural graft replacement using our neo-adventitia technique...
December 2017: Korean Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/29234606/complete-genome-sequence-of-n2-fixing-model-strain-klebsiella-sp-nov-m5al-which-produces-plant-cell-wall-degrading-enzymes-and-siderophores
#8
Zhili Yu, Shuying Li, Yuanyuan Li, Zhuang Jiang, Jinru Zhou, Qianli An
The bacterial strain M5al is a model strain for studying the molecular genetics of N2-fixation and molecular engineering of microbial production of platform chemicals 1,3-propanediol and 2,3-butanediol. Here, we present the complete genome sequence of the strain M5al, which belongs to a novel species closely related to Klebsiella michiganensis. M5al secretes plant cell wall-degrading enzymes and colonizes rice roots but does not cause soft rot disease. M5al also produces siderophores and contains the gene clusters for synthesis and transport of yersiniabactin which is a critical virulence factor for Klebsiella pathogens in causing human disease...
March 2018: Biotechnology Reports
https://www.readbyqxmd.com/read/29234225/a-new-surgical-technique-of-intra-scleral-tube-fixation-in-ahmed-glaucoma-valve-implantation-scleral-sleeve-method
#9
Charudutt Kalamkar, Nishant Radke, Amrita Mukherjee, Snehal Radke
Purpose: To present a new surgical technique 'Scleral Sleeve Method' which would reduce the risk of Ahmed Glaucoma Valve (AGV) tube related complications in patients undergoing AGV FP7 (New World Medical Inc., Rancho Cucamonga, CA) for refractory glaucoma. Design: Prospective, Non-Randomized, hospital based interventional case series. Subjects: A total of 16 eyes of adult patients (10 males and 6 females) with refractory glaucoma. Methods: Instead of using sutures to fix the AGV tube to sclera, we devised a novel method of intra-scleral tube fixation by creating a scleral tunnel fashioned in form of sleeve...
October 2017: Saudi Journal of Ophthalmology: Official Journal of the Saudi Ophthalmological Society
https://www.readbyqxmd.com/read/29233005/unimolecular-dissociation-of-anions-derived-from-succinic-acid-h2su-in-the-gas-phase-hsu-and-clmgsu-relationship-to-co2-fixation
#10
Joakim S Jestilä, Einar Uggerud
Electrospray ionization of mixtures of succinic acid (here denoted H2Su) and magnesium chloride in water/methanol give rise to ions of the type ESu- (E = H or ClMg). The unimolecular dissociation of these ions was studied by collisionally induced dissociation mass spectrometry and interpreted by quantum chemical calculations (density functional theory and the composite Gaussian-4 method) of relevant parts of the potential energy surfaces. The major dissociation pathways from HSu- were seen to be dehydration and decarboxylation, while ClMgSu- mainly undergoes decarboxylation...
January 1, 2017: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/29232750/-a-novel-technique-for-the-preparation-of-a-3-or-5-stranded-hamstring-graft-for-individualised-single-bundle-acl-or-pcl-reconstruction
#11
Philip Peter Rößler, Karl-Friedrich Schüttler, Turgay Efe
Objective Development of a preparation technique for hamstring tendons to ensure a maximum of intraoperative flexibility during individualised cruciate ligament surgery. Indications Primary ACL- and PCL-reconstruction as well as cases of later revision. Method Three- or five-stranded grafts can be obtained with the use of semitendinosus and gracilis tendons with fiber tape enforcement and use of a conventional femoral cortical suspensory fixation device. Fibre tape and knotting style ensure free motion of the continous loop...
December 2017: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/29232748/-combined-posteromedial-and-anterolateral-approach-in-the-floating-position-two-column-fracture-of-the-tibia-head
#12
Maike Reul, Harm Hoekstra
Background The posterior tibial plateau is often affected in intra-articular tibial fractures. Moreover, treating these posterior fractures is recognised as an important prognostic factor. Open reduction and internal fixation of lateral and posterior two column tibial plateau fractures can be achieved via a combined reversed L-shape approach and an anterolateral approach in the floating position without intraoperative repositioning of the patient. Material and Methods The operative procedure starts in the posterior position with visualisation of the posterior column after lateral retraction of the medial head of the M...
December 2017: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/29232333/structural-analysis-and-comprehensive-surgical-outcomes-of-the-sutureless-intrascleral-fixation-of-secondary-intraocular-lenses-in-human-eyes
#13
Bozho Todorich, Maxwell S Stem, Keshav Kooragayala, Aristomenis Thanos, Lisa J Faia, George A Williams, Tarek S Hassan, Maria A Woodward, Jeremy D Wolfe
PURPOSE: To describe surgical outcomes and structural characteristics of intraocular lenses (IOLs) implanted with transconjunctival sutureless intrascleral (SIS) fixation in human eyes. DESIGN: Retrospective interventional surgical case series involving live and cadaveric human eyes. METHODS: In this study, we investigated the surgical outcomes and structural anatomy of secondary IOLs implanted with the SIS technique in human eyes. All cases involving SIS IOL fixation performed at a single academic center from January 1, 2012, through July 30, 2016, were reviewed to describe the surgical technique, common indications, clinical outcomes, and the rate of common operative complications...
December 11, 2017: Retina
https://www.readbyqxmd.com/read/29232267/concomitant-anterior-repair-preoperative-prolapse-severity-and-anatomic-prolapse-outcomes-after-vaginal-apical-procedures
#14
Charles W Nager, Cara L Grimes, Tracy L Nolen, Clifford Y Wai, Linda Brubaker, Peter C Jeppson, Tracey S Wilson, Anthony G Visco, Matthew D Barber, Gary Sutkin, Peggy Norton, Charles R Rardin, Lily Arya, Dennis Wallace, Susan F Meikle
OBJECTIVE: The aim of the study was to compare anterior and overall prolapse prevalence at 1 year in surgical participants with or without concomitant anterior repair (AR) at the time of sacrospinous ligament fixation (SSLF) or uterosacral ligament suspension (ULS). METHODS: This is a secondary analysis of two surgical trials; concomitant AR was performed at surgeon's discretion. Anterior anatomic success was defined as pelvic organ prolapse quantification of prolapse point Ba ≤0 and overall success was defined as pelvic organ prolapse quantification points Ba, Bp, and C ≤0 at 12 months...
December 11, 2017: Female Pelvic Medicine & Reconstructive Surgery
https://www.readbyqxmd.com/read/29232194/persistent-ventricular-bigeminy-during-anesthesia-in-pediatric-patients-a-case-report-of-an-11-year-old-child
#15
Sheetal Dalal, Vikas Chaudhari
An 11-year-old male child with fractures in both bones in his left forearm presented for open reduction and internal fixation. The pre-anesthetic check-up and investigations did not reveal any pre-existing underlying cardio-respiratory disease. The patient had an uneventful peri-operative period during the operation and was comfortable without any anxiety or restlessness. After an uneventful induction and intubation as per routine protocol, the patient received 600 mg of amoxicillin+clavunate intravenously as an antibiotic...
December 12, 2017: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/29232186/cemented-or-cementless-total-knee-arthroplasty-comparative-results-of-200-cases-at-a-minimum-follow-up-of-11-years
#16
Jean-Louis Prudhon, Régis Verdier
INTRODUCTION: Since 1996 we have been using cementless fixation with hydroxyapatite (HA) coating. The purpose of this paper is to compare survivorship of a series of 100 cemented Total Knee Arthroplasty (TKA) to a similar series of 100 cementless with a follow up of 11-16 years. Material methods: Both TKA are mobile bearing total knee postero-stabilized. They can be used with cement or without cement. Among 1030 New Wave TKATM implanted from 2002 to 2015 we have identified 100 cemented TKAs and 100 cementless TKAs...
2017: SICOT-J
https://www.readbyqxmd.com/read/29232164/effect-of-ct-on-management-plan-in-malleolar-ankle-fractures
#17
Ashwani Kumar, Puneet Mishra, Anupama Tandon, Rajesh Arora, Manish Chadha
BACKGROUND: Conventionally ankle fractures have been classified using plain radiographs. Because of complex 3-dimensional anatomy and complexity of injuries, plain radiographs may not always be able to clearly depict the complete fracture pattern. There is a paucity of studies regarding the utility of computed tomography (CT) scanning in malleolar ankle fractures (MAFs). Hence, we conducted this study to further understand the role of the CT scan in MAFs. METHODS: A prospective study of 56 consecutive malleolar ankle fractures was conducted...
November 1, 2017: Foot & Ankle International
https://www.readbyqxmd.com/read/29231851/enhanced-methods-to-estimate-the-efficiency-of-magnetic-nanoparticles-in-imaging
#18
Ann M Hirt, Monika Kumari, David Heinke, Alexander Kraupner
Magnetic resonance imaging (MRI) and magnetic particle imaging (MPI) are powerful methods in the early diagnosis of diseases. Both imaging techniques utilize magnetic nanoparticles that have high magnetic susceptibility, strong saturation magnetization, and no coercivity. FeraSpinTM R and its fractionated products have been studied for their imaging performances; however, a detailed magnetic characterization in their immobilized state is still lacking. This is particularly important for applications in MPI that require fixation of magnetic nanoparticles with the target cells or tissues...
December 12, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29231437/-effect-and-mechanism-of-electroacupuncture-for-postoperative-ileus-at-perioperative-stage
#19
Junfan Fang, Wen Wang, Xiaomei Shao, Junying Du, Jianqiao Fang, Zhe Liu
OBJECTIVE: To observe the intervention effect of electroacupuncture (EA) on small intestinal motility in the rats of postoperative ileus (POI) at perioperative stage and explore the mechanism on the regulation of interstitial cells of Cajal (ICC) in the treatment of POI. METHODS: Sixty heathy male SD rats were randomized into a sham-operation group, a model group, an EA group and a sham-EA group, 15 rats in each one. Except the sham-operation group, POI modeling was done in the rest groups...
March 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
https://www.readbyqxmd.com/read/29231021/-eye-movement-characteristics-of-cooperation-degree-during-image-completion-test-in-psychiatric-impairment-assessment
#20
J J Wang, C Liu, L Liu, S Y Zhang, H Z Li, W X Cai
OBJECTIVES: To explore the difference of eye movement characteristics between uncooperative and cooperative subjects with mental disorder after cerebral trauma. METHODS: Thirty-nine subjects which needed psychiatric impairment assessment were selected. According to the binomial forced-choice digit memory test (BFDMT), all subjects were divided into cooperative and uncooperative groups. The subjects were asked to take the image completion test from Wechsler adult intelligence scale...
April 2017: Fa Yi Xue za Zhi
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