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https://www.readbyqxmd.com/read/29054057/removing-ethinylestradiol-from-wastewater-by-microalgae-mutant-chlorella-py-zu1-with-co2-fixation
#1
Jun Cheng, Qing Ye, Ke Li, Jianzhong Liu, Junhu Zhou
Removal of endocrine-disrupting chemical ethinylestradiol (EE2) from wastewater by microalgal mutant Chlorella PY-ZU1 under 15% CO2 were investigated. Moreover, the effects of EE2 on microscopic structure and antioxidation ability of microalgal cells were determined. EE2 concentrations in range of 0.01-5 mg L(-1) stimulated microalgal growth. Increasing the original EE2 concentration from 0 to 5 mg L(-1) increased the cell fractal dimension from 1.38 to 1.59 and reduced the cell size from 5.18 to 3...
October 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29053852/genome-wide-scan-and-analysis-of-positive-selective-signatures-in-dwarf-brown-egg-layers-and-silky-fowl-chickens
#2
Mengmeng Zhang, Liu Yang, Zhencheng Su, Muzhen Zhu, Wenting Li, Keliang Wu, Xuemei Deng
Chinese domestic chickens have been routinely subjected to artificial selection for the production of meats and eggs. Selection results in distinctive signatures in the genome that can reveal the genes underlying phenotypes of interest to breeders. In this study, we used the Chicken60K SNP chip to analyze DNA from Dwarf Brown-egg Layers (DB, n = 203) and Silky Fowls (SF, n = 181) and then examined the relative extended haplotype homozygosity (REHH) and fixation index (FST) to detect selection signatures. Since population structure analysis showed that SF was stratified into 2 subpopulations (SF1 and SF2), we removed the 20 SF1 chickens, and the remaining individuals (DB and SF2) were scanned for genome-wide selection signatures...
October 5, 2017: Poultry Science
https://www.readbyqxmd.com/read/29053753/3d-morphological-analysis-of-the-mouse-cerebral-vasculature-comparison-of-in-vivo-and-ex-vivo-methods
#3
Joe Steinman, Margaret M Koletar, Bojana Stefanovic, John G Sled
Ex vivo 2-photon fluorescence microscopy (2PFM) with optical clearing enables vascular imaging deep into tissue. However, optical clearing may also produce spherical aberrations if the objective lens is not index-matched to the clearing material, while the perfusion, clearing, and fixation procedure may alter vascular morphology. We compared in vivo and ex vivo 2PFM in mice, focusing on apparent differences in microvascular signal and morphology. Following in vivo imaging, the mice (four total) were perfused with a fluorescent gel and their brains fructose-cleared...
2017: PloS One
https://www.readbyqxmd.com/read/29053542/biomechanical-comparison-of-intrapelvic-and-extrapelvic-fixation-for-acetabular-fractures-involving-the-quadrilateral-plate
#4
Gregory J Gillispie, Sharon N Babcock, Kyle P McNamara, Michaela E Dimoff, Arun Aneja, Philip J Brown, Eben A Carroll
OBJECTIVES: Elderly patients represent the fastest growing and most difficult to treat population sustaining acetabular fractures. When treated surgically, isolated extrapelvic or combined intrapelvic-extrapelvic constructs may be used. No biomechanical or clinical study has compared the merits of these 2 techniques in cadaveric models. This research aims to biomechanically quantify the additional benefit of intrapelvic fixation to a standard extrapelvic fixation construct. METHODS: Ten cadaveric pelves underwent standardized anterior column and quadrilateral plate fracture creation...
November 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/29053502/the-community-orthopaedic-surgeon-taking-trauma-call-pediatric-ankle-fracture-pearls-and-pitfalls
#5
Shital N Parikh, Charles T Mehlman
Pediatric ankle fractures are common and have unique fracture characteristics because of the presence of distal tibial and fibular physes. When displaced (>3 mm widening of the physis or >2 mm intra-articular gap/step-off), these fractures are typically treated with anatomical reduction and internal fixation. Computed tomography is recommended for preoperative evaluation and surgical planning for intra-articular fractures. These fractures in younger children with substantial growth remaining should be followed closely to monitor for any growth disturbance...
November 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/29053500/the-community-orthopaedic-surgeon-taking-trauma-call-pediatric-femoral-shaft-fracture-pearls-and-pitfalls
#6
J Eric Gordon, Charles T Mehlman
Pediatric femoral shaft fractures present many challenging patient care decisions. Nonoperative treatment approaches still dominate care of the youngest age groups while surgical intervention is commonplace in virtually all older children. Treatment is determined primarily by patient age as modified by clinical factors including mechanism of injury, weight, and skeletal maturity. Infants can be successfully treated by placement into a Pavlik harness, whereas children younger than 5 years are most commonly treated by early reduction and spica cast immobilization...
November 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/29053434/alpha-gal-inactivated-heart-valve-bioprostheses-exhibit-an-anti-calcification-propensity-similar-to-knockout-tissues
#7
Filippo Naso, Ugo Stefanelli, Edward Buratto, Giovanna Lazzari, Andrea Perota, Cesare Galli, Alessandro Gandaglia
BACKGROUND: Glutaraldehyde (GLA) has been used to crosslink bioprosthetic heart valve (BHVs) tissues to enhance their stability, besides ensuring a satisfactory degree of immunological tolerance. Unfortunately, GLA fixation does not guarantee a complete tissue biocompatibility of BHVs in currently used devices. The interaction between preformed human anti-alpha-Gal antibody and alpha-Gal antigens promotes the calcification of GLA-treated alpha-Gal-positive tissue. Recently, an alarming correlation between the presence of the alpha-Gal epitope and a premature BHVs degeneracy was reported...
October 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29053148/stress-response-of-a-marine-ammonia-oxidizing-archaeon-informs-physiological-status-of-environmental-populations
#8
Wei Qin, Shady A Amin, Rachel A Lundeen, Katherine R Heal, Willm Martens-Habbena, Serdar Turkarslan, Hidetoshi Urakawa, Kyle C Costa, Erik L Hendrickson, Tony Wang, David Ac Beck, Sonia M Tiquia-Arashiro, Fred Taub, Andrew D Holmes, Neeraja Vajrala, Paul M Berube, Todd M Lowe, James W Moffett, Allan H Devol, Nitin S Baliga, Daniel J Arp, Luis A Sayavedra-Soto, Murray Hackett, E Virginia Armbrust, Anitra E Ingalls, David A Stahl
High representation by ammonia-oxidizing archaea (AOA) in marine systems is consistent with their high affinity for ammonia, efficient carbon fixation, and copper (Cu)-centric respiratory system. However, little is known about their response to nutrient stress. We therefore used global transcriptional and proteomic analyses to characterize the response of a model AOA, Nitrosopumilus maritimus SCM1, to ammonia starvation, Cu limitation and Cu excess. Most predicted protein-coding genes were transcribed in exponentially growing cells, and of ~74% detected in the proteome, ~6% were modified by N-terminal acetylation...
October 20, 2017: ISME Journal
https://www.readbyqxmd.com/read/29052829/esin-and-k-wire-fixation-have-similar-results-in-pediatric-both-bone-diaphyseal-forearm-fractures
#9
Namık Şahin, Yavuz Akalın, Oğuz Türker, Güven Özkaya
BACKGROUND: The purpose of this study was to compare short-term radiographic and clinical results of pediatric both-bone diaphyseal forearm fractures treated with intramedullary nail fixation using titanium nails or K-wires. METHODS: This was a prospective comparative trial. In total, 43 patients with both-bone open or closed forearm fractures who underwent surgical treatment with intramedullary fixation were randomly classified into two groups. Three patients did not return for the follow-up and were excluded from the study...
September 2017: Ulusal Travma Ve Acil Cerrahi Dergisi, Turkish Journal of Trauma & Emergency Surgery: TJTES
https://www.readbyqxmd.com/read/29052827/primary-exploration-for-radial-nerve-palsy-associated-with-unstable-closed-humeral-shaft-fracture
#10
Soo Hong Han, In Tae Hong, Ho Jae Lee, Sang June Lee, Uk Kim, Dong Won Kim
BACKGROUND: The treatment of radial nerve palsy caused by closed humeral shaft fracture is a matter of debate. The purpose of this study is to evaluate the outcome of early surgical management of radial nerve palsy in patients with unstable closed humeral shaft fractures and to determine whether patients with this injury should be surgically explored. METHODS: Medical records of 15 consecutive patients who had undergone open reduction and internal fixation for unstable closed humeral shaft fractures were reviewed...
September 2017: Ulusal Travma Ve Acil Cerrahi Dergisi, Turkish Journal of Trauma & Emergency Surgery: TJTES
https://www.readbyqxmd.com/read/29052754/development-of-a-method-for-the-detection-of-polystyrene-microplastics-in-paraffin-embedded-histological-sections
#11
Cátia Gonçalves, Marta Martins, Maria H Costa, Pedro M Costa
The concerns about the presence of microplastics (MPs) in marine ecosystems have widely increased in the past years. This is reflected in a growing number of studies addressing the effects of exposure to these materials in indigenous, farmed and even laboratory marine animals subjected to toxicity-oriented bioassays. There have been, however, many constraints in the detection of MPs in biological tissues, as routine histological techniques tend to degrade these materials, which are especially sensitive to organic solvents...
October 20, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29052314/modeling-the-convergence-accommodation-of-stereo-vision-for-binocular-endoscopy
#12
Yuanqian Gao, Jinhua Li, Jianmin Li, Shuxin Wang
BACKGROUND: The stereo laparoscope is an important tool for achieving depth perception in robot-assisted minimally invasive surgery (MIS). METHODS: A dynamic convergence accommodation algorithm is proposed to improve the viewing experience and achieve accurate depth perception. Based on the principle of the human vision system, a positional kinematic model of the binocular view system is established. The imaging plane pair is rectified to ensure that the two rectified virtual optical axes intersect at the fixation target to provide immersive depth perception...
October 19, 2017: International Journal of Medical Robotics + Computer Assisted Surgery: MRCAS
https://www.readbyqxmd.com/read/29052206/cell-type-specific-chromatin-analysis-in-whole-mount-plant-tissues-by-immunostaining
#13
Wenjing She, Célia Baroux, Ueli Grossniklaus
Chromatin organization in eukaryotes is highly dynamic, playing fundamental roles in regulating diverse nuclear processes including DNA replication, transcription, and repair. Thus, the analysis of chromatin organization is of great importance for the elucidation of chromatin-mediated biological processes. Immunostaining coupled with imaging is one of the most powerful tools for chromatin analysis at the cellular level. However, in plants, it is sometimes technically challenging to apply this method due to the inaccessibility of certain cell types and/or poor penetration of the reagents into plant tissues and cells...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29052166/is-human-classification-by-experienced-untrained-observers-a-gold-standard-in-fixation-detection
#14
Ignace T C Hooge, Diederick C Niehorster, Marcus Nyström, Richard Andersson, Roy S Hessels
Manual classification is still a common method to evaluate event detection algorithms. The procedure is often as follows: Two or three human coders and the algorithm classify a significant quantity of data. In the gold standard approach, deviations from the human classifications are considered to be due to mistakes of the algorithm. However, little is known about human classification in eye tracking. To what extent do the classifications from a larger group of human coders agree? Twelve experienced but untrained human coders classified fixations in 6 min of adult and infant eye-tracking data...
October 19, 2017: Behavior Research Methods
https://www.readbyqxmd.com/read/29052035/why-a-surgically-treated-humeral-shaft-fracture-became-a-nonunion-review-of-11%C3%A2-years-in-two-trauma-centers
#15
A Maresca, P Sangiovanni, S Cerbasi, R Politano, R Fantasia, M Commessatti, R Pascarella
AIM: The aim of this study was to evaluate nonunion causes of surgically treated humeral shaft fractures in two different trauma centers. METHODS: A total of 327 cases of humeral shaft fractures were treated in 11 years in two trauma centers. We retrospectively reviewed in detail some factors in order to understand the reasons for nonunion: (1) fracture type, according to the AO classification, (2) grade of open fracture, according to Gustilo-Anderson, (3) timing, (4) reduction and (5) fixation...
October 19, 2017: Musculoskeletal Surgery
https://www.readbyqxmd.com/read/29052010/open-versus-closed-treatment-of-distal-tibia-physeal-fractures-a-systematic-review-and-meta-analysis
#16
Waleed A Asad, Manaf H S Younis, Abdulaziz F Ahmed, Talal Ibrahim
AIMS: Distal tibia physeal fractures can lead to growth complications such as premature physeal closure (PPC), angular deformity and leg length discrepancy. The aim of our study was to systematically review the literature to assess whether open reduction and internal fixation (ORIF) is associated with lower rates of PPC compared to closed treatment. MATERIALS AND METHODS: We searched several databases from 1966 to 2016 for studies that evaluated ORIF versus closed treatment of distal tibia physeal fractures...
October 19, 2017: European Journal of Orthopaedic Surgery & Traumatology: Orthopédie Traumatologie
https://www.readbyqxmd.com/read/29052000/results-of-pectus-excavatum-correction-using-a-minimally-invasive-approach-with-subxyphoid-incision-and-three-point-fixation
#17
Sheldon J Bond, Emily Rapstine, Jordan M Bond
OBJECTIVES: This study reviews the results of our previously described modification of the minimally invasive (Nuss) procedure for correction of pectus excavatum. It utilizes a subxyphoid incision with central fixation to maximize safe bar passage and minimize bar displacement. METHODS: Consecutive patients corrected with the modified Nuss procedure between 2010 and 2015 form the basis of this study. RESULTS: During the study period, 73 patients had correction of their pectus excavatum by the modified Nuss procedure, utilizing subxyphoid incision and central fixation...
October 19, 2017: Pediatric Surgery International
https://www.readbyqxmd.com/read/29051899/surgical-treatment-of-segond-fractures-in-acute-anterior-cruciate-ligament-reconstruction
#18
Andrea Ferretti, Edoardo Monaco, Megan Rianne Wolf, Matteo Guzzini, Angelo De Carli, Daniele Mazza
BACKGROUND: The Segond fracture is an avulsion-type fracture located on the anterolateral aspect of the tibia associated with a ligament recently termed the anterolateral ligament. This injury has been described as pathognomonic for an anterior cruciate ligament (ACL) tear and is associated with a higher grade pivot shift. PURPOSE: To present and report 2-year clinical outcomes of a surgical technique for acute ACL reconstruction with fixation of a concomitant Segond fracture...
October 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/29051888/triplane-ankle-fracture-with-concomitant-ipsilateral-shaft-of-tibia-fracture-case-report-and-review-of-literature
#19
Sarang Kasture, Koldo Azurza
INTRODUCTION: Triplane fracture is a rare adolescent ankle injury. Its association with ipsilateral tibial shaft fracture is very rarely reported in the literature. CASE REPORT: This is a case of a 14-year-old male who sustained a twisting injury to his right leg after a fall from a height. He sustained a minimally displaced spiral fracture of the mid-shaft of his right tibia with a concomitant ipsilateral two part triplane fracture of the ankle. He underwent minimally invasive percutaneous locking plate fixation for tibial fracture and percutaneous screw fixation for an ankle fracture...
May 2017: Journal of Orthopaedic Case Reports
https://www.readbyqxmd.com/read/29051887/uncommon-floating-knee-in-a-teenager-a-case-report-of-ipsilateral-physeal-fractures-in-distal-femur-and-proximal-tibia
#20
Youssef Othman, Lassaad Hassini, Aymen Fekih, Issam Aloui, Abderrazek Abid
INTRODUCTION: The concomitance of ipsilateral physeal fractures of the distal femur and the proximal tibia is an extremely scarce entity. It is conceptually similar to floating knee in pediatric population. CASE REPORT: One case with this injury is reported in a 16-year-old teenager. He was treated surgically by close reduction and internal fixation. The diagnosis of the tibial fracture was initially missed and the fracture was seen on the post-operative radiographs...
May 2017: Journal of Orthopaedic Case Reports
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