keyword
MENU ▼
Read by QxMD icon Read
search

Sox2

keyword
https://www.readbyqxmd.com/read/28332752/bovine-trophectoderm-cells-induced-from-bovine-fibroblasts-with-induced-pluripotent-stem-cell-reprogramming-factors
#1
Neil C Talbot, Wendy O Sparks, Caitlin Phillips, Alan D Ealy, Anne M Powell, Thomas J Caperna, Wesley M Garrett, David M Donovan, Le Ann Blomberg
Thirteen independent induced bovine trophectroderm (iBT) cell lines were established by reprogramming bovine fetal liver-derived fibroblasts after viral-vector transduction with either 6 or 8 factors, including POU5F1 (OCT4), KLF4, SOX2, MYC, NANOG, LIN28, SV40 large T antigen, and hTERT.. Light- and electron-microscopy analysis showed that the iBT cells had epithelial cell morphology typical of bovine trophectoderm cells. Reverse-transcription-PCR assays indicated that all of the cell lines expressed interferon-tau (IFNT) at passages 1 or 2...
March 23, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28331164/immortalized-prairie-vole-derived-fibroblasts-vmf-k4dts-can-be-transformed-into-pluripotent-stem-cells-and-provide-a-useful-tool-with-which-to-determine-optimal-reprogramming-conditions
#2
Masafumi Katayama, Takashi Hirayama, Tohru Kiyono, Manabu Onuma, Tetsuya Tani, Satoru Takeda, Katsuhiko Nishimori, Tomokazu Fukuda
The cellular conditions required to establish induced pluripotent stem cells (iPSCs), such as the number of reprogramming factors and/or promoter selection, differ among species. The establishment of iPSCs derived from cells of previously unstudied species therefore requires the extensive optimization of programming conditions, including promoter selection and the optimal number of reprogramming factors, through a trial-and-error approach. While the four Yamanaka factors Oct3/4, Sox2, Klf4, and c-Myc are sufficient for iPSC establishment in mice, we reported previously that six reprogramming factors were necessary for the creation of iPSCs from primary prairie vole-derived cells...
March 23, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28323540/identification-of-multipotent-stem-cells-in-human-brain-tissue-following-stroke
#3
Kotaro Tatebayashi, Yasue Tanaka, Akiko Nakano-Doi, Rika Sakuma, Saeko Kamachi, Manabu Shirakawa, Kazutaka Uchida, Hiroto Kageyama, Toshinori Takagi, Shinichi Yoshimura, Tomohiro Matsuyama, Takayuki Nakagomi
Perivascular regions of the brain harbor multipotent stem cells. We previously demonstrated that brain pericytes near blood vessels also develop multipotency following experimental ischemia in mice, and that these ischemia-induced multipotent stem cells (iSCs) can contribute to neurogenesis. However, it is essential to understand the traits of iSCs in the post-stroke human brain for possible applications in stem cell-based therapies for stroke patients. Here we report for the first time that iSCs can be isolated from the post-stroke human brain...
March 21, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28321397/the-identification-of-three-cancer-stem-cell-subpopulations-within-moderately-differentiated-lip-squamous-cell-carcinoma
#4
Rachna Ram, Helen D Brasch, Jonathan C Dunne, Paul F Davis, Swee T Tan, Tinte Itinteang
AIM: To identify and characterize cancer stem cells (CSCs) in moderately differentiated lip squamous cell carcinoma (MDLSCC). METHOD: MDLSCC samples underwent 3,3-diaminobenzidine (DAB) immunohistochemical (IHC) staining for squamous cell carcinoma marker EMA, CSC marker CD44 and embryonic stem cell markers NANOG, octamer-binding transcription factor 4 (OCT4), spalt-like transcription factor 4 (SALL4), sex-determining region Y-box 2 (SOX2), and phosphorylated signal transducer and activator of transcription 3 (pSTAT3)...
2017: Frontiers in Surgery
https://www.readbyqxmd.com/read/28319578/podoplanin-and-sox2-expression-in-cin-3-like-squamous-cell-carcinoma-of-the-cervix
#5
Colin J R Stewart, Maxine L Crook
Squamous cell carcinoma (SCC) of the uterine cervix occasionally demonstrates a deceptive growth pattern that mimics endocervical crypt involvement by cervical intraepithelial neoplasia, grade 3 (CIN 3). Such CIN 3-like SCCs may be misinterpreted as noninvasive or minimally invasive leading to delays in diagnosis. Little is known of the factors that influence the growth patterns of cervical SCC but we suggested recently that CIN 3-like tumors might demonstrate "collective cellular invasion," which is characterized by a retained epithelial phenotype...
March 17, 2017: International Journal of Gynecological Pathology
https://www.readbyqxmd.com/read/28316792/jak-stat-pathway-activation-in-response-to-spinal-cord-injury-in-regenerative-and-non-regenerative-stages-of-xenopus-laevis
#6
Victor S Tapia, Mauricio Herrera-Rojas, Juan Larrain
Xenopus laevis tadpoles can regenerate the spinal cord after injury but this capability is lost during metamorphosis. Comparative studies between pre-metamorphic and metamorphic Xenopus stages can aid towards understanding the molecular mechanisms of spinal cord regeneration. Analysis of a previous transcriptome-wide study suggests that, in response to injury, the JAK-STAT pathway is differentially activated in regenerative and non-regenerative stages. We characterized the activation of the JAK-STAT pathway and found that regenerative tadpoles have an early and transient activation...
February 2017: Regeneration
https://www.readbyqxmd.com/read/28314692/identification-of-an-atypical-etiological-head-and-neck-squamous-carcinoma-subtype-featuring-the-cpg-island-methylator-phenotype
#7
K Brennan, J L Koenig, A J Gentles, J B Sunwoo, O Gevaert
Head and neck squamous cell carcinoma (HNSCC) is broadly classified into HNSCC associated with human papilloma virus (HPV) infection, and HPV negative HNSCC, which is typically smoking-related. A subset of HPV negative HNSCCs occur in patients without smoking history, however, and these etiologically 'atypical' HNSCCs disproportionately occur in the oral cavity, and in female patients, suggesting a distinct etiology. To investigate the determinants of clinical and molecular heterogeneity, we performed unsupervised clustering to classify 528 HNSCC patients from The Cancer Genome Atlas (TCGA) into putative intrinsic subtypes based on their profiles of epigenetically (DNA methylation) deregulated genes...
March 1, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28314379/rejuvenation-by-partial-reprogramming-of-the-epigenome
#8
Andrew R Mendelsohn, James Larrick, Jennifer L Lei
Epigenetic variation with age is one of the most important hallmarks of aging. Resetting or repairing the epigenome of aging cells in intact animals may rejuvenate the cells and perhaps the entire organism. In fact, differentiated adult cells, which by definition have undergone some epigenetic changes, are capable of being rejuvenated and reprogrammed to create pluripotent stem cells and viable cloned animals. Apparently, such reprogramming is capable of completely resetting the epigenome. However, attempts to fully reprogram differentiated cells in adult animals have failed in part because reprogramming leads to formation of teratomas...
March 17, 2017: Rejuvenation Research
https://www.readbyqxmd.com/read/28314267/induced-pluripotent-stem-cell-related-genes-contribute-to-de-differentiation-in-oral-squamous-cell-carcinoma
#9
Daisuke Takeda, Takumi Hasegawa, Takeshi Ueha, Eiji Iwata, Risa Harada, Akiko Sakakibara, Teruya Kawamoto, Tsutomu Minamikawa, Yoshitada Sakai, Takahide Komori
BACKGROUND/AIM: Cancer stem cells are suspected to contribute to malignancy in tumors. Hypoxia affects cell differentiation and induces stem-cell-like characteristics in malignancies. Induced pluripotency was demonstrated in mouse fibroblasts by reprogramming with four transcriptional factors: Oct3/4, Sox2, c-Myc, and Klf4. Conversely, oncogenic transformations frequently express transcriptional factors and Nanog. Therefore, cancer cells present some similarities with induced pluripotent stem (iPS) cells...
March 2017: Anticancer Research
https://www.readbyqxmd.com/read/28300292/hdac1-and-hdac3-underlie-dynamic-h3k9-acetylation-during-embryonic-neurogenesis-and-in-schizophrenia-like-animals
#10
Josef Večeřa, Eva Bártová, Jana Krejčí, Soňa Legartová, Denisa Komůrková, Jana Rudá-Kučerová, Tibor Štark, Eva Dražanová, Tomáš Kašpárek, Alexandra Šulcová, Frank J Dekker, Wiktor Szymanski, Christian Seiser, Georg Weitzer, Raphael Mechoulam, Vincenzo Micale, Stanislav Kozubek
Although histone acetylation is one of the most widely studied epigenetic modifications, there is still a lack of information regarding how the acetylome is regulated during brain development and pathophysiological processes. We demonstrate that the embryonic brain (E15) is characterized by an increase in H3K9 acetylation as well as decreases in the levels of HDAC1 and HDAC3. Moreover, experimental induction of H3K9 hyperacetylation led to the overexpression of NCAM in the embryonic cortex and depletion of Sox2 in the subventricular ependyma, which mimicked the differentiation processes...
March 16, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28297580/a-human-corneal-epithelial-cell-line-model-for-limbal-stem-cell-biology-and-limbal-immunobiology
#11
Bakiah Shaharuddin, Sajjad Ahmad, Nani Md Latar, Simi Ali, Annette Meeson
Limbal stem cell (LSC) deficiency is a visually debilitating condition caused by abnormal maintenance of LSCs. It is treated by transplantation of donor-derived limbal epithelial cells (LECs), the success of which depends on the presence and quality of LSCs within the transplant. Understanding the immunobiological responses of these cells within the transplants could improve cell engraftment and survival. However, human corneal rings used as a source of LSCs are not always readily available for research purposes...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28288641/sox2-regulates-multiple-malignant-processes-of-breast-cancer-development-through-the-sox2-mir-181a-5p-mir-30e-5p-tusc3-axis
#12
Kuancan Liu, Fuan Xie, Anding Gao, Rui Zhang, Long Zhang, Zhangwu Xiao, Qiong Hu, Weifeng Huang, Qiaojia Huang, Baoshun Lin, Jian Zhu, Haikun Wang, Jianwen Que, Xiaopeng Lan
BACKGROUND: High levels of SOX2 protein are correlated with increased dissemination of breast cancer. However, the underlying molecular mechanisms are not fully understood. METHODS: In this study we investigate the role of SOX2 in breast cancer metastasis using multiple in vitro and in vivo assays including cell culture, shRNA-mediated knockdown, wound healing, colony formation, transwell chamber, xenograft and tail vein injection. Moreover, western blot, immunostaining, microarray and real-time PCR were used to determine the change of protein and miRNA levels...
March 14, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28286162/gli1-expression-in-cancer-stem-like-cells-predicts-poor-prognosis-in-patients-with-lung-squamous-cell-carcinoma
#13
Yan Cui, Chun-Ai Cui, Zhao-Ting Yang, Wei-Dong Ni, Yu Jin, Yan-Hua Xuan
PURPOSE: Glioma-associated oncogene homolog 1 (Gli1) is involved in cancer stem cell (CSC) maintenance in various tumors; however, its expression and clinical significance in lung squamous cell carcinoma (LSCC) has not been reported. In this study, we aimed to reveal the clinical significance of Gli1 in LSCC and investigate the potential of Gli1 as a CSC marker by comparing its expression with that of other stemness-related genes in LSCC. METHODS: We assessed the expressions of Gli1, LSD1, CD44, Sox9 and Sox2 by immunohistochemistry in the tissue specimens obtained from 101 patients with LSCC...
March 9, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28286110/fast-transdifferentiation-of-human-wharton-s-jelly-mesenchymal-stem-cells-into-neurospheres-and-nerve-like-cells
#14
A R Bonilla-Porras, C Velez-Pardo, M Jimenez-Del-Rio
BACKGROUND: The human mesenchymal stem cells derived from Wharton's jelly tissue (hWJ-MSCs) represent a tool for cell-based therapies and regenerative medicine. hWJ-MSCs form neurospheres (NSs) within 3-7 days. No data is available to establish the neuro-phenotypic markers and time of formation of nerve-like (NLCs) and glial cells from NSs derived from hWJ-MSCs. NEW METHOD: hWJ-MSCs were incubated with Fast-N-Spheres medium for 24 and 72h. The new formed NSs were in turn incubated with forskolin in neurogenic NeuroForsk medium for 1-7days...
March 8, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28285878/stem-cell-factors-based-identification-and-functional-properties-of-in%C3%A2-vitro-selected-subpopulations-of-malignant-mesothelioma-cells
#15
Walter Blum, László Pecze, Emanuela Felley-Bosco, Licun Wu, Marc de Perrot, Beat Schwaller
Malignant mesothelioma (MM) is an aggressive neoplasm characterized by a poor patient survival rate, because of rapid tumor recurrence following first-line therapy. Cancer stem cells (CSCs) are assumed to be responsible for initiating tumorigenesis and driving relapse after therapeutic interventions. CSC-enriched MM cell subpopulations were identified by an OCT4/SOX2 reporter approach and were characterized by (1) increased resistance to cisplatin, (2) increased sensitivity toward the FAK inhibitor VS-6063 in vitro, and (3) a higher tumor-initiating capacity in vivo in orthotopic xenograft and allograft mouse models...
March 9, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28284480/copy-number-variations-found-in-patients-with-a-corpus-callosum-abnormality-and-intellectual-disability
#16
Solveig Heide, Boris Keren, Thierry Billette de Villemeur, Sandra Chantot-Bastaraud, Christel Depienne, Caroline Nava, Cyril Mignot, Aurélia Jacquette, Eric Fonteneau, Elodie Lejeune, Corinne Mach, Isabelle Marey, Sandra Whalen, Didier Lacombe, Sophie Naudion, Caroline Rooryck, Annick Toutain, Cédric Le Caignec, Damien Haye, Laurence Olivier-Faivre, Alice Masurel-Paulet, Christel Thauvin-Robinet, Fabien Lesne, Anne Faudet, Dorothée Ville, Vincent des Portes, Damien Sanlaville, Jean-Pierre Siffroi, Marie-Laure Moutard, Delphine Héron
OBJECTIVE: To evaluate the role that chromosomal micro-rearrangements play in patients with both corpus callosum abnormality and intellectual disability, we analyzed copy number variations (CNVs) in patients with corpus callosum abnormality/intellectual disability STUDY DESIGN: We screened 149 patients with corpus callosum abnormality/intellectual disability using Illumina SNP arrays. RESULTS: In 20 patients (13%), we have identified at least 1 CNV that likely contributes to corpus callosum abnormality/intellectual disability phenotype...
March 8, 2017: Journal of Pediatrics
https://www.readbyqxmd.com/read/28280847/neuronal-differentiation-capability-of-nasal-polyps-of-chronic-rhinosinusitis
#17
Michael Koennecke, Robert Böscke, Ann-Christin Pfannerstill, Stefan Reers, Martina Elsner, Benjamin Fell, Anja Richter, Karl-Ludwig Bruchhage, Sandra Schumann, Ralph Pries, Ludger Klimek, Barbara Wollenberg
Chronic rhinosinusitis with nasal polyps is considered a subgroup of chronic rhinosinusitis and a significant health problem, but the pathogenesis remains unclear to date. Therefore, we investigated the stemness to determine the role of stem cells in nasal polyps, with additional analysis of the neuronal differentiation potential of nasal polyp cells. We determined gene and protein expression profiles of stem cells in nasal polyp tissues, using whole genome microarray, quantitative real-time PCR (qPCR), immunohistochemistry, and flow cytometry...
March 9, 2017: Archivum Immunologiae et Therapiae Experimentalis
https://www.readbyqxmd.com/read/28276172/ascorbic-acid-promotes-the-stemness-of-corneal-epithelial-stem-progenitor-cells-and-accelerates-epithelial-wound-healing-in-the-cornea
#18
Jialin Chen, Jie Lan, Dongle Liu, Ludvig J Backman, Wei Zhang, Qingjun Zhou, Patrik Danielson
High concentration of ascorbic acid (vitamin C) has been found in corneal epithelium of various species. However, the specific functions and mechanisms of ascorbic acid in the repair of corneal epithelium are not clear. In this study, it was found that ascorbic acid accelerates corneal epithelial wound healing in vivo in mouse. In addition, ascorbic acid enhanced the stemness of cultured mouse corneal epithelial stem/progenitor cells (TKE2) in vitro, as shown by elevated clone formation ability and increased expression of stemness markers (especially p63 and SOX2)...
March 9, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28276156/retinoic-acid-inducible-gene-1-protein-rig1-like-receptor-pathway-is-required-for-efficient-nuclear-reprogramming
#19
Nazish Sayed, Frank Ospino, Farhan Himmati, Jieun Lee, Palas Chanda, Edward S Mocarski, John P Cooke
We have revealed a critical role for innate immune signaling in nuclear reprogramming to pluripotency, and in the nuclear reprogramming required for somatic cell transdifferentiation. Activation of innate immune signaling causes global changes in the expression and activity of epigenetic modifiers to promote epigenetic plasticity. In our previous papers, we focused on the role of toll-like receptor 3 (TLR3) in this signaling pathway. Here we define the role of another innate immunity pathway known to participate in the response to viral RNA, the retinoic acid-inducible gene 1 receptor (RIG-1)-like receptor (RLR) pathway...
March 9, 2017: Stem Cells
https://www.readbyqxmd.com/read/28273548/bone-marrow-mesenchymal-stem-cells-regulate-stemness-of-multiple-myeloma-cell-lines-via-btk-signaling-pathway
#20
Pan Zhao, Yafang Chen, Zhijie Yue, Ying Yuan, Xiaofang Wang
Bone marrow mesenchymal stem cells (BM-MSCs) are key components of bone marrow microenvironment. Although the importances of BM-MSCs activation in myeloma cells growth, development, progression, angiogenesis are well known, their role in the regulation of myeloma stemness is unclear. In this study, myeloma cell lines (LP-1, U266) were co-cultured with BM-MSCs, we found that BM-MSCs could up-regulate the expression of key stemness genes and proteins (OCT4, SOX2, NANOG) and increase clonogenicity. Similarly, the mechanisms underlying the BM-MSC activation of myeloma stemness remain unclear...
February 24, 2017: Leukemia Research
keyword
keyword
3632
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"