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Blood coagulation

Debnath Maji, Maria De La Fuente, Erdem Kucukal, Ujjal D S Sekhon, Alvin H Schmaier, Anirban Sen Gupta, Umut A Gurkan, Marvin T Nieman, Evi X Stavrou, Pedram Mohseni, Michael A Suster
BACKGROUND: Rapid point-of-care (POC) assessment of hemostasis is clinically important in patients with a variety of coagulation factor and platelet defects who have bleeding disorders. OBJECTIVE: To evaluate a novel dielectric microsensor, termed ClotChip, which is based on the electrical technique of dielectric spectroscopy for rapid, comprehensive assessment of whole blood coagulation. METHODS: The ClotChip is a three-dimensional, parallel-plate, capacitive sensor integrated into a single-use microfluidic channel with miniscule sample volume (<10 μL)...
July 14, 2018: Journal of Thrombosis and Haemostasis: JTH
Xu Shen, Dan Liu, Yong Lin, Xiao-Zhen Zhu, Li-Rong Lin, Man-Li Tong, Xian-Ming Liang, Li-Li Liu, Tian-Ci Yang, Jian-Jun Niu
Anticardiolipin antibody (ACA) includes beta2-glycoprotein I-dependent (β2-GPI-dependent) and β2-GPI-independent forms. The appearance of β2-GPI-dependent ACA and its association with blood coagulation have never been investigated in subjects with classical biological false-positive syphilis reactions (CBFP). In total, 146 CBFP subjects, 465 syphilis patients and 64 presumed antiphospholipid antibody syndrome (pAPS) patients were enrolled, and β2-GPI-dependent ACA IgA/IgG/IgM and anti-β2-GPI IgA/IgG/IgM antibodies were detected via chemiluminescence assay...
July 10, 2018: International Immunopharmacology
Burcu B Minsky, Rinat R Abzalimov, Chendi Niu, Yunlong Zhao, Zachary Kirsch, Paul L Dubin, Sergey N Savinov, Igor A Kaltashov
Inhibition of factor Xa (fXa) by antithrombin (AT) enabled by heparin or heparan sulfate is critical for controlling blood coagulation. AT activation by heparin had been investigated extensively, while heparin interaction with the trapped AT/fXa intermediates received relatively little attention. We use native electrospray ionization mass spectrometry to study the role of heparin chains of varying length (hexa-, octa-, deca- and eicosa-saccharides; dp6, dp8, dp10 and dp20) in AT/fXa complexes assembly. Despite being critical promoters of the AT/Xa binding, shorter heparin chains are excluded from the final products (trapped intermediates)...
July 12, 2018: Biochemistry
Mario Concha P, Verónica Mertz K, Gabriel Muñoz Castillo, Alejandro Delfino Y, Luis Cortínez F, Rodrigo Montaña R, Juan Cristóbal Pedemonte T, Ricardo Fuentes H
BACKGROUND: Compared to standard coagulation essays (SCE), such as international normalized ratio (INR), prothrombin activated partial thromboplastin time (aPTT), or platelet count, thromboelastograhy (TEG) offers precise and real-time information about hemostasis. TEG tests both platelet function and coagulation by assaying several parameters of clot formation dynamically in whole blood. AIM: To evaluate hemostasis in cirrhotic patients undergoing liver transplantation and determine the positive and negative predictive values of SCE for alterations of TEG...
March 2018: Revista Médica de Chile
Miriam Corredor, Daniel Carbajo, Cecilia Domingo, Yolanda Perez, Jordi Bujons, Angel Messeguer, Ignacio Alfonso
Despite heparin being the most widely used macromolecular drug, the design of small-molecule ligands to modulate its effects has been hampered by the structural properties of this polyanionic polysaccharide. In this work we used a dynamic covalent selection approach to identify a new ligand for heparin, assembled from extremely simple building blocks. The amplified molecule strongly binds to heparin (KD in the low μM range, ITC) by a combination of electrostatic, hydrogen bonding and CH-π interactions as shown by NMR and molecular modeling...
July 12, 2018: Angewandte Chemie
Alexandra Sherman, Moanaro Biswas, Roland W Herzog
PURPOSE OF REVIEW: Hemophilia is an X-linked blood coagulation genetic disorder, which can cause significant disability. Replacement therapy for coagulation factor VIII (hemophilia A) or factor IX (hemophilia B) may result in the development of high-affinity alloantibodies ('inhibitors') to the replacement therapy, thus making it ineffective. Therefore, there is interest in directing immunological responses towards tolerance to infused factors. RECENT FINDINGS: In this review, we will discuss latest advancements in the development of potentially less immunogenic replacement clotting factors, optimization of current tolerance induction protocols (ITI), preclinical and clinical data of pharmacological immune modulation, hepatic gene therapy, and the rapidly advancing field of cell therapies...
July 9, 2018: Current Opinion in Hematology
Toshiko Tanaka, Angelique Biancotto, Ruin Moaddel, Ann Zenobia Moore, Marta Gonzalez-Freire, Miguel A Aon, Julián Candia, Pingbo Zhang, Foo Cheung, Giovanna Fantoni, Richard D Semba, Luigi Ferrucci
To characterize the proteomic signature of chronological age, 1,301 proteins were measured in plasma using the SOMAscan assay (SomaLogic, Boulder, CO, USA) in a population of 240 healthy men and women, 22-93 years old, who were disease- and treatment-free and had no physical and cognitive impairment. Using a p ≤ 3.83 × 10-5 significance threshold, 197 proteins were positively associated, and 20 proteins were negatively associated with age. Growth differentiation factor 15 (GDF15) had the strongest, positive association with age (GDF15; 0...
July 11, 2018: Aging Cell
Malin Björkander, Peter Bentzer, Ulf Schött, Marcus E Broman, Thomas Kander
BACKGROUND: Incidence and risk factors for complications after insertion of central venous catheters have previously been reported for smaller cohorts. The aim of this observational multicenter study was to study risk factors for mechanical complications in a large, recently collected cohort of patients. METHODS: Records of central venous catheter insertions from 8 hospitals in southern Sweden from 2013 to 2016 were collected from the regional chart system. Data on blood coagulation tests, use of ultrasonography, central venous catheter location, bore size, number of needle passes, arterial puncture, the chronological order of the central venous catheter insertion, and mechanical complications were extracted...
July 11, 2018: Acta Anaesthesiologica Scandinavica
Raúl E Cian, Antonela G Garzón, Olga Martínez-Augustin, Cecilia C Botto, Silvina R Drago
Antithrombotic activity of brewers' spent grain peptides before and after simulated gastrointestinal digestion and their effects on blood coagulation pathways were evaluated. Two hydrolysates were produced using sequential enzymatic systems: alkaline protease + Flavourzyme (AF) and neutral protease + Flavourzyme (PF). Simulation of gastrointestinal digestion of AF and PF hydrolysates was made using porcine pepsin and pancreatin enzymes, obtaining the corresponding digested samples: AFD and PFD, respectively...
July 10, 2018: Plant Foods for Human Nutrition
Andreas Hohn, Ivan Iovino, Fabrizio Cirillo, Hendrik Drinhaus, Kathrin Kleinbrahm, Lennert Boehm, Edoardo De Robertis, Jochen Hinkelbein
During the last years, proteomic studies have revealed several interesting findings in experimental sepsis models and septic patients. However, most studies investigated protein alterations only in single organs or in whole blood. To identify possible sepsis biomarkers and to evaluate the relationship between protein alteration in sepsis affected organs and blood, proteomics data from the heart, brain, liver, kidney, and serum were analysed. Using functional network analyses in combination with hierarchical cluster analysis, we found that protein regulation patterns in organ tissues as well as in serum are highly dynamic...
2018: BioMed Research International
I-Ting Peng, Ming-Ting Chung, Ching-Chung Lin
Postpartum hemorrhage remains a major threat to maternal health. Intervention after critical blood loss or development of disseminated intravascular coagulation may lead to disastrous organ failure and poor outcomes. A 30-year-old woman was transferred to our emergency department due to massive postpartum hemorrhage. Shock and disseminated intravascular coagulation ensued, and the patient's condition quickly deteriorated. We performed an emergency hysterectomy, but blood loss had been massive. Moreover, there was another episode of internal bleeding that led to further blood loss...
2018: Case Reports in Obstetrics and Gynecology
Luis J Serrano, Ana Cañete, Tamara Garcia-Leal, Laura Tomás-Gallardo, Ana I Flores, Paz de la Torre, Antonio Liras, María José Sánchez
The development of new strategies based on cell therapy approaches to correct haemophilia A (HA) requires further insights into new cell populations capable of producing coagulation factor VIII (FVIII) and presenting stable engraftment potential. The major producers of FVIII in the adult are liver sinusoidal endothelial cells (LSECs) and in a lesser degree bone marrow-derived cells, both of which have been shown to ameliorate the bleeding phenotype in adult HA mice after transplantation. We have previously shown that cells from the foetal liver (FL) and the aorta-gonads-mesonephros (AGM) haematopoietic locations possess higher LSEC engraftment potential in newborn mice compared with adult-derived LSECs, constituting likely therapeutic targets for the treatment of HA in neonates...
July 10, 2018: Thrombosis and Haemostasis
Xianbin Kong, Jingjing Zhang, Jingrui Huo, Lei Wang, Lei Guo, Ying Liu, Tao He, Zhonglei Sun, Xuyi Chen, Zhenjiang Hou, Xiaohui Yang, Yi Tian, Shizhong Sun, Feng Chen, Yingfu Liu
Immunosuppression is an important mechanism for the development of sepsis pathology, and is the key to the high mortality of sepsis. However, patients appear to be immunocompromised before sepsis onset due to lack of enough attention. Present sepsis models cannot fully mimic the onset of sepsis in patients. Hence, effective treatments in animal experiments could not be transformed into clinical application. In the present study, we improved the animal model of sepsis and used cyclosporine A immunosuppressive mice to make it closer to immune status before the onset of sepsis, followed by the intraperitoneal injection of Escherichia coli (E...
July 5, 2018: International Immunopharmacology
Fabian B Fahlbusch, Thomas Heinlein, Manfred Rauh, Sven Dittrich, Robert Cesnjevar, Julia Moosmann, Jennifer Nadal, Matthias Schmid, Frank Muench, Michael Schroth, Wolfgang Rascher, Hans-Georg Topf
OBJECTIVES: Coagulation factor XIII (FXIII) plays a key role in fibrin clot stabilization-an essential process for wound healing following cardiothoracic surgery. However, FXIII deficiency as a risk for post-operative bleeding in pediatric cardiac surgery involving cardiopulmonary bypass (CPB) for congenital heart disease (CHD) is controversially discussed. Thus, as primary outcome measures, we analyzed the association of pre-operative FXIII activity and post-operative chest tube drainage (CTD) loss with transfusion requirements post-operatively...
2018: PloS One
Lewis Fall, Julien Brugniaux, Danielle Hodson, Christopher Marley, Bruce Davies, Karl New, Jane McEneny, Ian S Young, Damian Miles Bailey
In-vitro evidence suggests that blood coagulation is activated by increased oxidative stress although the link and underlying mechanism in humans has yet to be established. We conducted the first randomised controlled trial to examine if oral antioxidant prophylaxis alters the haemostatic responses to hypoxia and exercise. Healthy males were randomly assigned double-blind to either an antioxidant (n = 20) or placebo group (n = 16). The antioxidant group ingested 2 capsules/day that each contained 500 mg of L-ascorbic acid and 450 international units (IU) of dl-α-tocopherol acetate for eight weeks...
July 10, 2018: Journal of Physiology
Ecaterina Scărlătescu, Marcus D Lancé, Nathan J White, Dana R Tomescu
: Sepsis induces alterations in blood coagulation which are associated with mortality. Using rotational thromboelastometry (ROTEM), these states are often characterized by changes in parameters representing terminal clot lysis. However, the ROTEM changes are often subtle, making them difficult to recognize and interpret. The objective of this study is to evaluate a new ROTEM clot velocity parameter representing clot formation kinetics in septic ICU patients. We hypothesized that this time-based parameter was significantly different in critically ill septic survivors vs...
July 4, 2018: Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis
Hiromichi Shiotsu, Kazuhiro Okada, Tatsuki Shibuta, Yuki Kobayashi, Saki Shirahama, Chieri Kuroki, Saori Ueda, Masanori Ohkuma, Katsuyoshi Ikeda, Yukio Ando, Hirotaka Matsui, Yuzo Kayamori, Tsukuru Umemura
1) Background: MicroRNAs (miRNA) are expected as useful biomarkers for various diseases. We studied the pre-analytical factors causing variation in the analysis of miRNA. 2) Material and Methods: Blood samples were collected from 25 healthy subjects. Plasma and serum were obtained from the same samples. The levels of miR-451, -16, -126, and -223 were analyzed using RT-qPCR. Cel-miR-39 was added as a spiked-in control in each sample. 3) Results: With the exception of miR-451, the levels of the miRNAs in plasma were higher than in serum...
July 9, 2018: MicroRNA
Jakob Schroder, Rikke Zethner-Moller, Kira Bang Bové, Naja Dam Mygind, Philip Hasbak, Marie Mide Michelsen, Ida Gustafsson, Jens Kastrup, Eva Prescott
BACKGROUND AND AIMS: While a plethora of biomarkers have been shown to be associated with coronary artery disease, studies assessing biomarkers in coronary microvascular dysfunction (CMD) are few. We investigated associations between cardiovascular protein biomarkers and non-endothelium dependent CMD assessed by positron emission tomography (PET). METHODS: In 97 women with angina pectoris and no significant obstructive coronary artery disease (<50% stenosis on invasive coronary angiography), CMD was defined as myocardial blood flow reserve (MBFR) < 2...
June 19, 2018: Atherosclerosis
Xi Song, Siyuan Cui, Zhentao Li, Yanpeng Jiao, Changren Zhou
Chitin/graphene oxide (Ch/GO) composite sponges had been synthesized in 11 wt% NaOH/4 wt% urea aqueous solution by a simple method. The structure, thermal stability and mechanical properties of the composite sponges were investigated by scanning electron microscopy, Fourier-transform infrared spectroscopy, wide-angle X-ray diffraction, thermogravimetric analysis, and compressive strength measurements. The results revealed that chitin and GO were mixed homogeneously. Interestingly, the composite sponges showed meso-macroporous structure, which played an important role in improving their adsorption properties...
July 6, 2018: Journal of Materials Science. Materials in Medicine
Christina Paidas Teefey, Celeste P Durnwald, Sindhu K Srinivas, Lisa D Levine
OBJECTIVE:  This article evaluates the differences in adverse maternal outcomes related to severe preeclampsia in obese versus nonobese women. STUDY DESIGN:  Retrospective cohort study and planned secondary analysis of women with severe preeclampsia comparing a composite adverse maternal outcome related to preeclampsia among obese (body mass index [BMI] ≥ 30 kg/m2 ) and nonobese (BMI < 30 kg/m2 ) women. The composite outcome was defined as ≥ 1 of the following prior to discharge: renal failure, liver abnormality, thrombocytopenia, blood transfusion, pulmonary edema, disseminated intravascular coagulation, stroke, or eclampsia...
July 6, 2018: American Journal of Perinatology
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