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https://www.readbyqxmd.com/read/29161769/influence-of-biceps-brachii-tendon-mechanical-properties-on-elbow-flexor-force-steadiness-in-young-and-old-males
#1
Rowan R Smart, Stéphane Baudry, Andrey Fedorov, Samantha L Kuzyk, Jennifer M Jakobi
Elbow flexor force steadiness (FS) depends on strength and decreases with age. Achilles tendon mechanics effect standing balance and isometric plantarflexion FS. This study investigated the influence of distal biceps brachii (BB) tendon mechanics and elbow flexor strength on age-related decline in FS. Nine young (23±2 years) and 9 old (77±5 years) males performed submaximal isometric elbow flexion tasks at low (2.5, 5, 10% maximal voluntary contraction (MVC)) and high (20, 40, 60, 80%MVC) forces in a neutral forearm position...
November 21, 2017: Scandinavian Journal of Medicine & Science in Sports
https://www.readbyqxmd.com/read/29161712/clostridium-difficile-infection-in-the-nephrology-ward
#2
Sylwia Dudzicz, Marcin Adamczak, Andrzej Więcek
Clostridium difficile is currently the most frequently identified pathogen causing antibiotic-associated diarrhea and the main cause of nosocomial diarrhea. In recent years, increases incidence of infection, severe infection, recurrent infection and mortality from Clostridium difficile infection (CDI) have been observed. This may be a consequence of excessive antibiotic use and spread of the hypervirulent epidemic BI/NAP1/027 strain of Clostridium difficile. The main risk factors for CDI are: antibiotic therapy, previous hospitalizations and number of comorbid conditions...
November 21, 2017: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/29161696/the-protein-expression-level-of-a-heterogeneous-gene-inserted-in-lipi-1-of-the-listeria-ivanovii-genome-relies-on-its-insertion-orientation
#3
Sijing Liu, Mingjuan Jiang, Lin Su, Tian Tang, Xiang Zhang, Yongyu Li, Qikang Pu, Chenyan Ren, Chuan Wang
Due to its capability to multiply in either phagocytic or nonphagocytic cells, and to subsequently elicit a robust cellular immune response, Listeria ivanovii (LI) is thought to be feasible for developing bacteria-based live attenuated vaccines. We previously generated several recombinant LI strains expressing Mycobacterium tuberculosis antigens. Since the expression level of heterogeneous protein was sometimes very low, we attempted to elucidate the principle of heterogeneous protein expression in such recombinant LI strains...
November 22, 2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29161685/intergenotype-recombination-among-new-norovirus-gii-4-variants-in-the-complete-genome
#4
Jinfeng Xu, Tingjun Liu, Hua Wang, Hongxing Shen
GII.4 noroviruses (NoVs) are a major cause of acute gastroenteritis in humans. A new variant of GII.4, the Sydney variant, has recently become more prevalent on a global scale. Intragenotype recombinations are widespread within the pandemic NoV GII.4 lineage, and are likely to be important forces driving the evolution and emergence of novel GII.4 viruses. In this study, we sought to examine the role that intergenotype recombination has played in the emergence of GII.4 Sydney 2012 variants. The results show that the GII...
November 22, 2017: Intervirology
https://www.readbyqxmd.com/read/29161679/the-changes-of-nutrient-composition-of-piled-laying-hen-manure-and-anaerobic-fermentation-for-recycling-as-a-dietary-ingredient-for-ruminants
#5
Tianlong Han, Liping Wang, Yanming Zhang, Jun Zhang, Dongsheng Han, Ning Lv, Xiaohua Han, Guoqiang Zhao, Min Wang
This study investigated the changes of nutrient compositions of piled laying hen manure, detected the physical and chemical components of laying hen manure fermented by mixed strains, and analysed its application effects on feeding ruminants. The results showed that with increasing of piling time, the contents of crude protein (CP) and ether extract (EE) in laying hen manure were reduced, while the contents of crude ash (CA), calcium (Ca) and phosphorus (P) were increased. Fermentation could effectively decrease pH value, reduce Ca/P ratio, lessen the total bacterial counts, and maintain the organisms contents in laying hen manure...
November 18, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/29161620/in-situ-elastic-strain-mapping-during-micromechanical-testing-using-ebsd
#6
Mark J McLean, William A Osborn
Compared to more commonly used strain measurement techniques, electron backscatter diffraction (EBSD) offers improved spatial resolution and measurement sensitivity. Additionally, EBSD can provide the full deformation tensor, whereas other techniques, such as digital image correlation (DIC), are limited to only in-plane strains and rotations. In this work, EBSD was used to measure strains and rotations in-situ during testing of a single-crystal silicon micromechanical test specimen. The theta-like specimen geometry was chosen due to the complex and spatially-varying strain states that exist in the circular frame of the sample during testing, as well as the nominally uniform strains in the central web...
November 14, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/29161571/effects-of-inorganic-carbon-on-the-nitrous-oxide-emissions-and-microbial-diversity-of-an-anaerobic-ammonia-oxidation-reactor
#7
Wenjie Zhang, Dunqiu Wang, Yue Jin
Inorganic carbon (IC) is important for anaerobic ammonium oxidation (anammox). In this study, the effects of the IC concentration on N2O emissions and microbial diversity in an anammox reactor were investigated. N2O emissions were positively correlated with IC concentrations, and IC concentrations in the range of 55-130 mg/L were optimal, considering the nitrogen removal rate and N2O emissions. High IC concentrations resulted in the formation of CaCO3 on the surface of anammox granules, which impacted the diffusion conditions of the substrate...
November 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29161506/multiplexed-crispr-cas9-genome-editing-and-gene-regulation-using-csy4-in-saccharomyces-cerevisiae
#8
Raphael Ferreira, Christos Skrekas, Jens Nielsen, Florian David
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology has greatly accelerated the field of strain engineering. However, insufficient efforts have been made towards developing robust multiplexing tools in Saccharomyces cerevisiae. Here, we exploit the RNA processing capacity of the bacterial endoribonuclease Csy4 from Pseudomonas aeruginosa, to generate multiple gRNAs from a single transcript for genome editing and gene interference applications in S. cerevisiae. In regards to genome editing, we performed a quadruple deletion of FAA1, FAA4, POX1 and TES1 reaching 96% efficiency out of 24 colonies tested...
November 21, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29161495/essential-oils-and-their-components-as-an-alternative-in-the-control-of-mosquito-vectors-of-disease
#9
Sergio Andrade Ochoa, Luvia E Sánchez-Torres, Guadalupe Virginia Nevárez-Moorillón, Alejandro D Camacho, Benjamín Nogueda-Torres
More than half of the human population is exposed to mosquito-borne infections. Climate change and the emergence of strains resistant to traditionally used insecticides have motivated the search of new agents for mosquito population control. Essential oils have been effective repellents and larvicidal agents.The aim of this work was to review research studies conducted in recent years on the larvicidal activity of essential oils and their components against Aedes, Anopheles and Culex mosquitoes, as well as the latest reports about their possible mechanism of action...
March 29, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29161470/resequencing-of-a-mutant-bearing-an-iron-starvation-recovery-phenotype-defines-slr1658-as-a-new-player-in-the-regulatory-network-of-a-model-cyanobacterium
#10
Hagit Zer, Ketty Margulis, Jens Georg, Yoram Shotland, Gergana Kostova, Laure Sultan, Wolfgang R Hess, Nir Keren
Photosynthetic microorganisms encounter an erratic nutrient environment characterized by periods of iron limitation and sufficiency. Surviving in such an environment requires mechanisms for handling these transitions. Our study identified a regulatory system involved in the process of recovery from iron limitation in cyanobacteria. We set out to study the role of bacterioferritin co-migratory proteins during transitions in iron bioavailability in the cyanobacterium Synechocystis sp. PCC 6803 using knockout strains coupled with physiological and biochemical measurements...
November 21, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29161422/prevalence-and-genetic-variability-of-human-polyomaviruses-6-and-7-in-healthy-skin-among-asymptomatic-individuals
#11
Yumiko Hashida, Tomonori Higuchi, Shigenobu Matsuzaki, Kimiko Nakajima, Shigetoshi Sano, Masanori Daibata
Background: Despite the pathogenetic potential of human polyomavirus 6 (HPyV6) and human polyomavirus 7 (HPyV7), they have been found in the normal skin of healthy individuals. However, little is known about the prevalence, infection levels, and geographical variations of these polyomaviruses in the skin. Methods: Using skin swabs from 470 participants aged 2-98 years, we estimated the prevalence of copy numbers of HPyV6 and HPyV7 with respect to age and ethnicity...
November 17, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29161329/comparative-metabolomics-profiling-of-engineered-saccharomyces-cerevisiae-lead-to-a-strategy-that-improving-%C3%AE-carotene-production-by-acetate-supplementation
#12
Xiao Bu, Liang Sun, Fei Shang, Guoliang Yan
A comparative metabolomic analysis was conducted on recombinant Saccharomyces cerevisiae strain producing β-carotene and the parent strain cultivated with glucose as carbon source using gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography-mass spectrometry (HPLC-MS) and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) based approach. The results showed that most of the central intermediates associated with amino acids, carbohydrates, glycolysis and TCA cycle intermediates (acetic acid, glycerol, citric acid, pyruvic acid and succinic acid), fatty acids, ergosterol and energy metabolites were produced in a lower amount in recombinant strain, as compared to the parent strain...
2017: PloS One
https://www.readbyqxmd.com/read/29161313/biomarkers-and-echocardiography-for-evaluating-the-improvement-of-the-ventricular-diastolic-function-after-surgical-relief-of-hydronephrosis
#13
Huei-Ming Yeh, Ting-Tse Lin, Chih-Fan Yeh, Ho-Shiang Huang, Sheng-Nan Chang, Jou-Wei Lin, Chia-Ti Tsai, Ling-Ping Lai, Yi-You Huang, Chun-Lin Chu
The pathophysiology of cardio-renal syndrome (CRS) is complex. Hydronephrosis caused by urolithiasis may cause cytokine release and lead to cardiac dysfunction. The aim of this study was to evaluate cardiac function changes observed in patients who received double J placement using feasible biomarkers and echocardiography. This was a prospective, single-center study. Eighty-seven patients who presented with acute unilateral hydronephrosis and received ureteroscope stone manipulation were enrolled. Echocardiography and cytokines were measured on the day of the operation and 24 hours after the procedure...
2017: PloS One
https://www.readbyqxmd.com/read/29161169/investigation-of-apoptosis-in-cultured-cells-infected-with-equine-herpesvirus-1
#14
M R Scrochi, C N Zanuzzi, N Fuentealba, F Nishida, M E Bravi, M E Pacheco, G H Sguazza, E J Gimeno, E L Portiansky, C I Muglia, C M Galosi, C G Barbeito
Many viruses alter different stages of apoptosis of infected cells as a strategy for successful infection. Few studies have addressed mechanisms of equine herpesvirus 1 (EHV-1) strain-induced cell death. We investigated the effect of an abortigenic strain (AR8 strain) on heterologous Madin-Darby bovine kidney cells and homologous equine dermis (ED) cells cell lines. We compared morphologic and biochemical features of early and late apoptosis at different postinfection times. We investigated translocation of phosphatidylserine to the cell surface, nuclear fragmentation and changes in the cytoskeleton using flow cytometry and annexin V/propidium iodide staining, DNA laddering, terminal deoxynucleotidyl transferase UTP nick-end labeling assay and immunofluorescence staining of cytokeratin 18 cleavage...
November 21, 2017: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29161121/research-design-and-analysis-of-a-low-stiffness-porous-hip-stem
#15
Ibrahim Eldesouky, Hassan El-Hofy, Ola Harrysson
Two major problems are associated with total hip replacement: 1) stress shielding and 2) the adverse tissue reaction to certain elements of the implant material. In this regard, a porous implant provides lower stiffness and vacancies for bone ingrowth, making it more suitable for the human bone compared with a solid stem. Moreover, second-generation titanium biomedical alloys, such as TNZT (Ti35Nb7Zr5Ta) and TMZF (Ti12Mo6Zr2Fe), have been introduced to prevent the adverse tissue reactions related to aluminum and vanadium elements of the popular Ti6Al4V alloy...
November 2017: Biomedical Instrumentation & Technology
https://www.readbyqxmd.com/read/29161048/local-chemical-ordering-and-negative-thermal-expansion-in-ptni-alloy-nanoparticles
#16
Qiang Li, He Zhu, Li Rong Zheng, Longlong Fan, Na Wang, Yangchun Rong, Yang Ren, Jun Chen, Jinxia Deng, Xianran Xing
An atomic insight into the local chemical ordering and lattice strain is particular interesting to recent emerging bimetallic nanocatalysts such as PtNi alloys. Here, we reported the atomic distribution, chemical environment and lattice thermal evolution in full-scale structural description of PtNi alloy nanoparticles (NPs). The different segregation of elements in the well-faceted PtNi nanoparticles is convinced by extended X-ray absorption fine structure (EXAFS). Atomic pair distribution function (PDF) study evidences the co-existence of the face-centered cubic and tetragonal ordering parts in the local environment of PtNi nanoparticles...
November 21, 2017: Nano Letters
https://www.readbyqxmd.com/read/29160983/eds-report-strong-surges-in-flu-related-volume-straining-capacity-in-some-regions
#17
David Burmeister, Mohamud Daya, Christopher Sampson
No abstract text is available yet for this article.
April 2017: ED Management: the Monthly Update on Emergency Department Management
https://www.readbyqxmd.com/read/29160926/electro-mechanical-response-of-a-3d-nerve-bundle-model-to-mechanical-loads-leading-to-axonal-injury
#18
I Cinelli, M Destrade, M Duffy, P McHugh
OBJECTIVE: Traumatic brain injuries and damage are major causes of death and disability. We propose a 3D fully coupled electro-mechanical model of a nerve bundle to investigate the electrophysiological impairments due to trauma at the cellular level. METHODS: The coupling is based on a thermal analogy of the neural electrical activity by using the finite element software Abaqus CAE 6.13-3. The model includes a real-time coupling, modulated threshold for spiking activation and independent alteration of the electrical properties for each 3-layer fibre within a nerve bundle as a function of strain...
November 21, 2017: International Journal for Numerical Methods in Biomedical Engineering
https://www.readbyqxmd.com/read/29160896/influence-of-fracture-stability-on-staphylococcus-epidermidis-and-staphylococcus-aureus-infection-in-a-murine-femoral-fracture-model
#19
M Sabaté Brescó, L O'Mahony, S Zeiter, K Kluge, M Ziegler, C Berset, D Nehrbass, R G Richards, T F Moriarty
Fracture-related infection (FRI) is a major complication in surgically fixed fractures. Instability of the fracture after fixation is considered a risk factor for infection; however, few experimental data are available confirming this belief. To study whether stable fractures led to higher infection clearance, mouse femoral osteotomies were fixed with either stable or unstable fixation and the surgical site was contaminated with either Staphylococcus epidermidis (S. epidermidis)or Staphylococcus aureus (S. aureus)clinical isolates...
November 21, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/29160864/clonal-expansion-behind-a-marine-diatom-bloom
#20
Maria Valeria Ruggiero, Domenico D'Alelio, Maria Immacolata Ferrante, Mariano Santoro, Laura Vitale, Gabriele Procaccini, Marina Montresor
Genetic diversity is what selection acts on, thus shaping the adaptive potential of populations. We studied micro-evolutionary patterns of the key planktonic diatom Pseudo-nitzschia multistriata at a long-term sampling site over 2 consecutive years by genotyping isolates with 22 microsatellite markers. We show that both sex and vegetative growth interplay in shaping intraspecific diversity. We document a brief but massive demographic and clonal expansion driven by strains of the same mating type. The analysis of an extended data set (6 years) indicates that the genetic fingerprint of P...
November 21, 2017: ISME Journal
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