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https://www.readbyqxmd.com/read/29220540/reflectance-confocal-microscopy-evaluation-of-trichobacteriosis-axilaris
#1
Andrea Combalia, Beatriz Alejo, Susana Puig
We read with great interest the paper by Navarrete-Dechent et al recently published on the Journal of European Academy of Dermatology1 , in which the authors detail the dermoscopic findings of trichobacteriosis and highlight the importance of dermoscopy in these cases. We present the case of a 44-year-old man, who emanated an unpleasant axilary odor during a routine check-up. Close examination of the axillary hair shafts showed soft, brown to yellow irregular masses adherent to the central segment of the hairs, suggesting the diagnosis of trichobacteriosis axilaris (TA)...
December 8, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/29218526/glut4-translocation-in-single-muscle-cells-in-culture-epitope-detection-by-immunofluorescence
#2
Javier R Jaldin-Fincati, Philip J Bilan, Amira Klip
GLUT4 is the major glucose transporter in skeletal muscle. GLUT4 cycles to and from the plasma membrane and its exocytic rate is accelerated by insulin and muscle contraction to achieve a new steady state with more GLUT4 proteins at the muscle cell surface. To gain a better understanding of the molecular and cellular mechanisms that govern GLUT4 protein recycling, we developed an in vitro model in which myc-epitope-tagged GLUT4 or GLUT4-GFP is expressed in L6 skeletal muscle cells. The myc-epitope is inserted into an exofacial domain that is accessible to anti-myc antibodies from the outside of non-permeabilized cells, allowing one to count the number of transporters at the cell surface...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29218030/in-situ-antibacterial-activity-of-essential-oils-with-and-without-alcohol-on-oral-biofilm-a-randomized-clinical-trial
#3
Victor Quintas, Isabel Prada-López, María J Carreira, David Suárez-Quintanilla, Carlos Balsa-Castro, Inmaculada Tomás
Currently, there is little evidence on the in situ antibacterial activity of essential oils (EO) without alcohol. This study aimed to evaluate in situ the substantivity and antiplaque effect on the plaque-like biofilm (PL-biofilm) of two solutions, a traditional formulation that contains EO with alcohol (T-EO) and an alcohol-free formulation of EO (Af-EO). Eighteen healthy adults performed a single mouthwash of: T-EO, Af-EO, and sterile water (WATER) after wearing an individualized disk-holding splint for 2 days...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29216889/targeting-the-cross-talk-between-urokinase-receptor-and-formyl-peptide-receptor-type-1-to-prevent-invasion-and-trans-endothelial-migration-of-melanoma-cells
#4
Concetta Ragone, Michele Minopoli, Vincenzo Ingangi, Giovanni Botti, Federica Fratangelo, Antonello Pessi, Maria Patrizia Stoppelli, Paolo Antonio Ascierto, Gennaro Ciliberto, Maria Letizia Motti, Maria Vincenza Carriero
BACKGROUND: Accumulating evidence demonstrates that the Urokinase Receptor (uPAR) regulates tumor cell migration through its assembly in composite regulatory units with transmembrane receptors, and uPAR88-92 is the minimal sequence required to induce cell motility through the Formyl Peptide Receptor type 1 (FPR1). Both uPAR and FPR1 are involved in melanoma tumor progression, suggesting that they may be targeted for therapeutic purposes. In this study, the role of the uPAR-FPR1 cross-talk to sustain melanoma cell ability to invade extracellular matrix and cross endothelial barriers is investigated...
December 8, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29216623/exosomal-microrna-mir-1246-promotes-cell-proliferation-invasion-and-drug-resistance-by-targeting-ccng2-in-breast-cancer
#5
Xiu Juan Li, Zhao Jun Ren, Jin Hai Tang, Qiao Yu
BACKGROUND/AIMS: Treatment of breast cancer remains a clinical challenge. This study aims to validate exosomal microRNA-1246 (miR-1246) as a serum biomarker for breast cancer and understand the underlying mechanism in breast cancer progression. METHODS: The expression levels of endogenous and exosomal miRNAs were examined by real time PCR, and the expression level of the target protein was detected by western blot. Scanning electron and confocal microscopy were used to characterize exosomes and to study their uptake and transfer...
December 6, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29215558/synthesis-of-benzyl-acetate-catalyzed-by-lipase-immobilized-in-nontoxic-chitosan-polyphosphate-beads
#6
Ana D Q Melo, Francisco F M Silva, José C S Dos Santos, Roberto Fernández-Lafuente, Telma L G Lemos, Francisco A Dias Filho
Enzymes serve as biocatalysts for innumerable important reactions, however, their application has limitations, which can in many cases be overcome by using appropriate immobilization strategies. Here, a new support for immobilizing enzymes is proposed. This hybrid organic-inorganic support is composed of chitosan-a natural, nontoxic, biodegradable, and edible biopolymer-and sodium polyphosphate as the inorganic component. Lipase B from Candida antarctica (CALB) was immobilized on microspheres by encapsulation using these polymers...
December 7, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29215373/mangiferin-prevents-the-growth-of-gastric-carcinoma-by-blocking-the-pi3k-akt-signalling-pathway
#7
Min Du, Gang Wen, Juan Jin, Yuanguang Chen, Jun Cao, Aman Xu
The aim of the present study was to investigate the effects of mangiferin on gastric carcinoma cells and to determine the possible mechanisms underlying such effects. The MTT assay was performed to evaluate the antiproliferative effect of mangiferin. Following treatment, apoptosis rates of SGC-7901 were established by flow cytometry and laser confocal microscopy, and western blot analysis was used to detect the expression of apoptosis-related proteins. The MTT assay showed that mangiferin inhibited the proliferation of SGC-7901 and BCG-823 cells in a dose-dependent and time-dependent manner...
December 5, 2017: Anti-cancer Drugs
https://www.readbyqxmd.com/read/29215357/new-technique-for-direct-fluoroimmunomagnetic-detection-of-rotavirus-in-water-samples
#8
Raquel A Villamizar-Gallardo, Johann F Osma, Oscar Orlando Ortíz
A new rapid, sensitive and selective method for rotavirus detection in water samples is described in this paper. Amino pink magnetic microparticles were functionalized with monoclonal antibodies and used to capture, concentrate, separate and detect infectious rotavirus particles in distilled and drinking water samples. The fluorescence of the microparticles was used to determine the presumptive presence of rotaviruses by using confocal microscopy. Atomic force microscopy and transmission electron microscopy were used to confirm the presence of the anti-rotavirus antibodies attached to the surface of the magnetic microparticles as well as that of viruses attached through the antibody...
October 2017: Journal of Water and Health
https://www.readbyqxmd.com/read/29215264/tuning-bacterial-attachment-and-detachment-via-the-thickness-and-dispersity-of-a-ph-responsive-polymer-brush
#9
Vivek Yadav, Yuly Andrea Jaimes-Lizcano, Narendra K Dewangan, Nayoung Park, Tzu-Han Li, Megan L Robertson, Jacinta C Conrad
We investigated the effect of two brush parameters, thickness and dispersity in the molecular weight distribution, on the adhesion of bacteria to pH-responsive poly(acrylic acid) (PAA) brushes synthesized using surface-initiated atom transfer radical polymerization. The attachment and detachment of Staphylococcus epidermidis to PAA brushes at pH 4 and pH 9, respectively, were examined with confocal microscopy. An optimal range of brush thickness, 13 - 18 nm, was identified for minimizing bacterial adhesion on PAA brushes at pH 4, and bacterial attachment did not depend on the brush dispersity...
December 7, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29214717/bisphenol-a-is-an-exogenous-toxin-that-promotes-mitochondrial-injury-and-death-in-tubular-cells
#10
Enrique Bosch-Panadero, Sebastian Mas, Esther Civantos, Pedro Abaigar, Vanesa Camarero, Alberto Ruiz-Priego, Alberto Ortiz, Jesus Egido, Emilio González-Parra
BACKGROUND: Uremic toxins that accumulate in chronic kidney disease (CKD) contribute to CKD complications, such as CKD progression. Bisphenol A (BPA) is a ubiquitous environmental toxin, structurally related with p-cresol, that accumulates in CKD. Our aim was to characterize the nephrotoxic potential of BPA. Specifically, we addressed BPA toxicity over energy-demanding proximal tubular cells. METHODS: Cell death and oxidative stress were evaluated by flow cytometry and confocal microscopy in HK-2 human proximal tubular epithelial cells...
December 7, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/29214708/chemicals-on-demand-photo-induced-release-from-nano-and-microparticle-containers
#11
Ulrich S Schubert, Christoph Englert, Ivo Nischang, Cornelia Bader, Philipp Borchers, Julien Alex, Michael Proehl, Martin Hentschel, Matthias Hartlieb, Anja Traeger, Georg Pohnert, Stephanie Schubert, Michael Gottschaldt
A benzoin-derived diol linker was synthesized and used to create biocompatible polyesters, which can be fully decomposed on demand triggered by UV light. Extensive structural optimization of the linker unit was performed to enable a defined encapsulation of structurally diverse organic compounds in polymeric structures and a well-controllable polymer cleavage process. Selective tracking of release kinetics of encapsulated model compounds from the polymeric nano- and microparticle containers was enabled by confocal laser scanning microscopy as a proof-of-principle for desired applications...
December 6, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29214310/tear-levels-of-insulin-like-growth-factor-binding-protein-3-correlate-with-subbasal-nerve-plexus-changes-in-patients-with-type-2-diabetes-mellitus
#12
Whitney L Stuard, Rossella Titone, Danielle M Robertson
Purpose: This study investigated the expression of insulin-like growth factor binding protein-3 (IGFBP-3) in basal tears of patients with type 2 diabetes mellitus compared to nondiabetic controls; and correlated tear levels of IGFBP-3 with morphologic changes in the subbasal nerve plexus and density of basal corneal epithelial cells. Methods: This was a single visit, cross-sectional study. Diabetic and control subjects were matched for age, sex, smoking status, and body mass index...
December 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29214256/thermally-reversible-nanoparticle-gels-with-tuneable-porosity-showing-structural-colour
#13
Z Ruff, P Cloetens, T O'Neill, C P Grey, E Eiser
We present colloidal gels formed from dispersions of PEG- and PEG+DNA-coated silica nanoparticles showing structural colour. The PEG- and PEG+DNA-coated silica colloids are functionalized using exclusively covalent bonds in aqueous conditions. Both sets of colloids self-assemble into thermally-reversible colloidal gels with porosity that can be tuned by changing the colloid volume fraction, although the interaction potentials of the colloids in the two systems are different. Confocal microscopy and image analysis tools are used to characteraize the gels' microstructures...
December 7, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29214243/intracellular-investigation-on-the-differential-effects-of-4-polyphenols-on-mcf-7-breast-cancer-cells-by-raman-imaging
#14
A Mignolet, B R Wood, E Goormaghtigh
The past decades have seen significant interest in the study of polyphenolic compounds as potential therapeutic agents in medicine because they display a vast array of cellular effects beneficial to treat or manage a plethora of chronic diseases including inflammatory diseases, cardiovascular abnormalities and several types of cancer. These compounds act at different stages of carcinogenesis but deciphering their mode of action is a complex task. Live MCF-7 breast cancer cells were investigated using Raman imaging to evaluate the perturbations induced after incubating cells with four different polyphenols: EGCG, gallic acid, resveratrol and tannic acid...
December 7, 2017: Analyst
https://www.readbyqxmd.com/read/29214113/reflectance-confocal-microscopy-of-mammary-paget-disease
#15
Fezal Ozdemir, Bengu G Turk, Banu Yaman, Giovanni Pellecani, Necmettin Ozdemir, Isil Karaarslan, Francesca Farnetani
Mammary Paget disease is the intraepidermal adenocarcinoma of the nipple area which is characterized usually by a well-demarcated eczematous plaque. Reflectance confocal microscopy (RCM), is an in vivo noninvasive diagnostic tool with a high-resolution imaging of the skin, almost comparable to conventional histology. RCM findings of Paget disease are limited in the literature. Most of the reported cases are the extramammary type. In this report, we aimed to evaluate the RCM findings of a non-pigmented mammary Paget disease in a 65 year-old woman...
October 2017: Dermatology Practical & Conceptual
https://www.readbyqxmd.com/read/29214109/a-solitary-pink-lesion-dermoscopy-and-rcm-features-of-lichen-planus
#16
Claudia Pezzini, Simonetta Piana, Caterina Longo, Elisa Benati, Stefania Borsari, Francesca Specchio, Elvira Moscarella
We present an unusual onset of cutaneous lichen planus (LP) in a middle-aged patient. The initial presentation as solitary, indolent pink lesion required further investigations to rule out malignancy, especially amelanotic melanoma. Dermoscopy and reflectance confocal microscopy findings were found to be helpful in our case in addressing the correct diagnosis.
October 2017: Dermatology Practical & Conceptual
https://www.readbyqxmd.com/read/29214108/reflectance-confocal-microscopy-of-an-inverted-follicular-keratosis-mimicking-a-squamous-cell-carcinoma
#17
Sarah Hocker, Harold S Rabinovitz, Margaret Oliviero, Jane Grant-Kels, Alon Scope
Distinguishing between benign and malignant neoplasms of the skin is a daily challenge to dermatologists. With the use of a dermatoscope and other imaging devices, the diagnosis is often more precise. The confocal microscope is a device that uses a near-infrared laser to perform noninvasive imaging of the skin. The benefit is that the images immediately provide additional, cellular-level information that can assist in diagnosis. However, lesions may share overlapping characteristics on confocal microscopy, and hence, benign lesions can still display confocal features concerning for a cancerous process, justifying a biopsy...
October 2017: Dermatology Practical & Conceptual
https://www.readbyqxmd.com/read/29214103/in-vivo-intraoral-reflectance-confocal-microscopy-of-an-amalgam-tattoo
#18
Oriol Yélamos, Miguel Cordova, Gary Peterson, Melissa P Pulitzer, Bhuvanesh Singh, Milind Rajadhyaksha, Jennifer L DeFazio
The majority of oral pigmentations are benign lesions such as nevi, melanotic macules, melanoacanthomas or amalgam tattoos. Conversely, mucosal melanomas are rare but often lethal; therefore, excluding oral melanomas in this setting is crucial. Reflectance confocal microscopy is a non-invasive, in vivo imaging system with cellular resolution that has been used to distinguish benign from malignant pigmented lesions in the skin, and more recently in the mucosa. However, lesions located posteriorly in the oral cavity are difficult to assess visually and difficult to biopsy due to their location...
October 2017: Dermatology Practical & Conceptual
https://www.readbyqxmd.com/read/29214101/cell-junction-protein-armadillo-repeat-gene-deleted-in-velo-cardio-facial-syndrome-is-expressed-in-the-skin-and-colocalizes-with-autoantibodies-of-patients-affected-by-a-new-variant-of-endemic-pemphigus-foliaceus-in-colombia
#19
Ana Maria Abreu-Velez, Hong Yi, Michael S Howard
Background: We previously described a new variant of endemic pemphigus foliaceus in El Bagre, Colombia, South America (El Bagre-EPF, or pemphigus Abreu-Manu). El Bagre-EPF differs from other types of EPF clinically, epidemiologically, immunologically and in its target antigens. We reported the presence of patient autoantibodies colocalizing with armadillo repeat gene deleted in velo-cardio-facial syndrome (ARVCF), a catenin cell junction protein colocalizing with El Bagre-EPF autoantibodies in the heart and within pilosebaceous units along their neurovascular supply routes...
October 2017: Dermatology Practical & Conceptual
https://www.readbyqxmd.com/read/29213312/adding-tetrahydrofuran-to-dilute-acid-pretreatment-provides-new-insights-into-substrate-changes-that-greatly-enhance-biomass-deconstruction-by-clostridium-thermocellum-and-fungal-enzymes
#20
Vanessa A Thomas, Bryon S Donohoe, Mi Li, Yunqiao Pu, Arthur J Ragauskas, Rajeev Kumar, Thanh Yen Nguyen, Charles M Cai, Charles E Wyman
Background: Consolidated bioprocessing (CBP) by anaerobes, such as Clostridium thermocellum, which combine enzyme production, hydrolysis, and fermentation are promising alternatives to historical economic challenges of using fungal enzymes for biological conversion of lignocellulosic biomass. However, limited research has integrated CBP with real pretreated biomass, and understanding how pretreatment impacts subsequent deconstruction by CBP vs. fungal enzymes can provide valuable insights into CBP and suggest other novel biomass deconstruction strategies...
2017: Biotechnology for Biofuels
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