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Hitendra S Chand, Yohannes A Mebratu, Marena Montera, Yohannes Tesfaigzi
BACKGROUND: Airway epithelial cells (AECs) are crucial for mucosal and adaptive immunity but whether these cells respond in a memory-dependent manner is poorly studied. Previously, we have reported that LPS intratracheal instillation in rodents causes extensive neutrophilic inflammation and airway epithelial cell hyperplasia accompanied by mucous cell metaplasia (MCM). And the resolution process required a period of 40 d for the inflammation to subside and the lung epithelia to resemble the non-exposed condition...
October 20, 2016: Respiratory Research
D Shormanov, E P Tsatsua, T F Koropova, K Astashkina, Intern Yangolenko
The objective of the present study was to elucidate the peculiarities of the determination of 2-chloro-1,4-dihydrooxybenzene in the tissues of different organs and blood. The analytical methods used in the study included TLC, GC-MS, and UF-spectrophotometry. The study has demonstrated the usefulness of using acetone as an insulating solvent for the extraction of 2-chloro-1,4-dihydrooxybenzene from the biological materials. It was shown that the compound being analyzed can be separated from the endogenous substances contained in the biological matrices on the "Silasorb C-18" columns, 30 mcm...
2016: Sudebno-meditsinskaia Ekspertiza
Friederike Finsterbusch, Ramya Ravindranathan, Ihsan Dereli, Marcello Stanzione, Daniel Tränkner, Attila Tóth
Orderly chromosome segregation during the first meiotic division requires meiotic recombination to form crossovers between homologous chromosomes (homologues). Members of the minichromosome maintenance (MCM) helicase family have been implicated in meiotic recombination. In addition, they have roles in initiation of DNA replication, DNA mismatch repair and mitotic DNA double-strand break repair. Here, we addressed the function of MCMDC2, an atypical yet conserved MCM protein, whose function in vertebrates has not been reported...
October 2016: PLoS Genetics
Simon Schimmack, Ben Lawrence, Barton Kenney, Hubertus Schmitz-Winnenthal, Irvin M Modlin, Mark Kidd
BACKGROUND: Small intestinal neuroendocrine neoplasm (SI-NEN) proliferation is quantified by Ki67 measurements which capture G1-G2M phases of the cell cycle. G0 and early G1 phases, typical of slow-growing cells, can be detected by minichromosome maintenance protein (MCM) expression. We hypothesized that these replication licensing markers may provide clinically relevant information to augment Ki67 in low-grade neuroendocrine neoplasia. METHODS: Immunohistochemical staining (IHC), Western blot analysis, quantitative polymerase chain reaction, and copy number variations of MCM2, MCM3, and Ki67 were undertaken in SI-NENs (n = 22)...
October 2016: Translational Oncology
Shenli Zhang, Maria Perez-Page, Kelly Guan, Erick Yu, Joseph W Tringe, Ricardo H R Castro, Roland Faller, Pieter Stroeve
Molecular Dynamics (MD) and Monte Carlo (MC) simulations were applied together for the first time to reveal the porous structure transformation mechanisms of mesoporous silica MCM-41 subjected to temperatures up to 2885 K. Silica was experimentally characterized to inform the models and enable prediction of changes in gas adsorption/separation properties. MD simulations suggest that the pore closure process is activated by a collective diffusion of matrix atoms into the porous region, accompanied by bond reformation at the surface...
October 17, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
Yohannes Yihdego, Len Drury
The efficient use of water resources in arid region has become highly relevant in the evaluation and mining planning, since the exploration phase to closure. The objective of the numerical groundwater model was to assess the potential for groundwater extraction to meet mine water demand from one of the driest area in the world. Numerical groundwater models were used to assess groundwater resource. Modelling was undertaken using MODFLOW-SURFACT code, an advanced MODFLOW based code, within the framework of Visual MODFLOW version 4...
November 2016: Environmental Monitoring and Assessment
Tony Karlsborn, A K M Firoj Mahmud, Hasan Tükenmez, Anders S Byström
INTRODUCTION: The Elongator complex, comprising six subunits (Elp1p-Elp6p), is required for formation of 5-carbamoylmethyl (ncm(5)) and 5-methoxycarbonylmethyl (mcm(5)) side chains on wobble uridines in 11 out of 42 tRNA species in Saccharomyces cerevisiae. Loss of these side chains reduces the efficiency of tRNA decoding during translation, resulting in pleiotropic phenotypes. Overexpression of hypomodified [Formula: see text], which in wild-type strains are modified with mcm(5)s(2)U, partially suppress phenotypes of an elp3Δ strain...
2016: Metabolomics: Official Journal of the Metabolomic Society
Ann P Quick, Qiongling Wang, Leonne E Philippen, Giselle Barreto-Torres, David Y Chiang, David L Beavers, Guoliang Wang, Maha Khalid, Julia O Reynolds, Hannah M Campbell, Jordan Showell, Mark D McCauley, Arjen Scholten, Xander H Wehrens
RATIONALE: Junctional membrane complexes (JMC) in myocytes are critical microdomains, in which excitation-contraction coupling occurs. Structural and functional disruption of JMCs underlies contractile dysfunction in failing hearts. However, the role of newly identified JMC protein striated muscle preferentially expressed gene (SPEG) remains unclear. OBJECTIVE: To determine the role of SPEG in healthy and failing adult hearts. MMethods and Results: Proteomic analysis of immunoprecipatated JMC-proteins ryanodine receptor type-2 (RyR2) and junctophilin-2 (JPH2) followed by mass spectrometry identified the serine-threonine kinase SPEG as the only novel binding partner for both proteins...
October 11, 2016: Circulation Research
Huayao Chen, Yueshun Lin, Hongjun Zhou, Xinhua Zhou, Sheng Gong, Hua Xu
The salicylaldehyde modified mesoporous silica (SA-MCM-41) was prepared through a co-condensation method. Through the bridge effect from copper ion which also acts as the nutrition of the plant, the model drug- chlorpyrifos(CH) was supported on the copper(II)/Schiff base mesoporous silica (Cu-MCM-41) to form a highly efficient sustained released system (CH-Cu-MCM-41) for pesticide delivery. The experimental results showed that the larger the concentration of copper ion was, the more adsorption capacity (AC) of Cu-MCM-41 for chlorpyrifos was and the smaller its release rate was...
October 7, 2016: Journal of Agricultural and Food Chemistry
Prem Prakash Kushwaha, Krishna Chaitanya Rapalli, Shashank Kumar
DNA replicates in a timely manner with each cell division. Multiple proteins and factors are involved in the initiation of DNA replication including a dynamic interaction between Cdc10-dependent transcript (Cdt1) and Geminin (GMNN). A conformational change between GMNN-Cdt1 heterotrimer and heterohexamer complex is responsible for licensing or inhibition of the DNA replication. This molecular switch ensures a faithful DNA replication during each S phase of cell cycle. GMNN inhibits Cdt1-mediated minichromosome maintenance helicases (MCM) loading onto the chromatin-bound origin recognition complex (ORC) which results in the inhibition of pre-replication complex assembly...
October 1, 2016: Biochimie
Alla Arakcheeva, Dmitry Chernyshov, Massimo Spina, László Forró, Endre Horváth
The crystal structure of the pristine (I) and aged (II) crystals of CH3NH3PbI3 (hereafter MAPbI3) hybrid organic-inorganic lead iodide has been studied at 293 K with high-precision single-crystal X-ray diffraction using a synchrotron light source. We show that (I) and (II) are characterized by an identical tetragonal unit cell but different space groups: I422 for (I) and P42212 for (II). Both space groups are subgroups of I4/mcm, which is widely used for MAPbI3. The main difference between (I) and (II) comes from the difference in hydrogen bonds between the MA(+) cation and the PbI3 framework which is the direct consequence of H2O insertion in the aged crystal (II)...
October 1, 2016: Acta Crystallographica Section B, Structural Science, Crystal Engineering and Materials
Vinicius P Fardin, Vitor Guarconi de Paula, Estevam A Bonfante, Paulo G Coelho, Gerson Bonfante
OBJECTIVE: To evaluate the fatigue life of zirconia-veneered and metal-ceramic crowns comprised by an even thickness or a modified framework design when loaded on marginal ridges. METHODS: Eighty marginal ridges were present after fabrication of forty molar crowns cemented onto composite-resin replicas and divided (n=20/each), in the following groups: metal-ceramic with even thickness (MCev) or with a modified framework design (MCm, lingual collar with proximal struts); porcelain-fused to zirconia with even thickness (PFZev) or with the modified framework design (PFZm)...
September 30, 2016: Dental Materials: Official Publication of the Academy of Dental Materials
Yun-Peng Peng, Yi Zhu, Ling-Di Yin, Jing-Jing Zhang, Song Guo, Yue Fu, Yi Miao, Ji-Shu Wei
OBJECTIVES: Minichromosome maintenance (MCM) proteins play important roles in DNA replication by interacting with other factors which participate in the regulation of DNA synthesis. Abnormal over-expression of MCMs was observed in numerous malignancies, such as colorectal cancer. However, the expression of MCMs in pancreatic cancer (PC) was less investigated so far. This study was designed to analyze the expression and prognostic roles of MCM1-10 in PC based on the data provided by The Cancer Genome Atlas (TCGA)...
2016: PloS One
Hui Wang, Songjin Zhang, Xiumei Tian, Chufeng Liu, Lei Zhang, Wenyong Hu, Yuanzhi Shao, Li Li
Nanoprobes for combined optical and magnetic resonance imaging have tremendous potential in early cancer diagnosis. Gold nanoparticles (AuNPs) co-doped with Gd2O3 mesoporous silica nanocomposite (Au/Gd@MCM-41) can produce pronounced contrast enhancement for T1 weighted image in magnetic resonance imaging (MRI). Here, we show the remarkably high sensitivity of Au/Gd@MCM-41 to the human poorly differentiated nasopharyngeal carcinoma (NPC) cell line (CNE-2) using fluorescence lifetime imaging (FLIM). The upconversion luminescences from CNE-2 and the normal nasopharyngeal (NP) cells (NP69) after uptake of Au/Gd@MCM-41 show the characteristic of two-photon-induced-radiative recombination of the AuNPs...
October 3, 2016: Scientific Reports
Ožbej Zupančič, Julia Anita Grießinger, Julia Rohrer, Irene Pereira de Sousa, Lukas Danninger, Alexandra Partenhauser, Nadine Flavia Elli Sündermann Laffleur, Andreas Bernkop-Schnürch
AIM: It was the aim of this study to develop SEDDS for oral enoxaparin administration and evaluate it in vitro and in vivo. METHODS: The emulsifying properties of SEDDS composed of long chain lipids (LC-SEDDS), medium chain lipids (MC-SEDDS), short chain lipids (SC-SEDDS) and no lipids (NL-SEDDS) were evaluated. Thereafter, enoxaparin was incorporated via hydrophobic ion pairing in the chosen SEDDS, which were evaluated regarding their mucus permeating properties, stability towards pancreatic lipase, drug release profile and cytotoxicity...
September 28, 2016: European Journal of Pharmaceutics and Biopharmaceutics
Xingqing Xiao, Binwu Zhao, Paul F Agris, Carol K Hall
In this paper, we investigate the ability of our computationally-designed peptide, Pept10 (PNWNGNRWLNNCLRG), to recognize the anticodon stem and loop (ASL) domain of the hypermodified tRNA(Lys3) (mcm(5) s(2) U34 ,ms(2) t(6) A37 ), a reverse transcription primer of HIV replication. Five other ASLs, the singly modified ASL(Lys3) (ms(2) t(6) A37 ), ASL(Lys3) (s(2) U34 ), ASL(Lys3) (Ψ39 ), ASL(Lys1,2) (t(6) A37 ), and ASL(Glu) (s(2) U34 ), were used as decoys. Explicit-solvent atomistic molecular dynamics simulations were performed to examine the process of binding of Pept10 with the target ASL(Lys3) (mcm(5) s(2) U34 ,ms(2) t(6) A37 ) and the decoy ASLs...
September 28, 2016: Protein Science: a Publication of the Protein Society
Rentian Wu, Zhiquan Wang, Honglian Zhang, Haiyun Gan, Zhiguo Zhang
DNA replication is tightly regulated to occur once and only once per cell cycle. How chromatin, the physiological substrate of DNA replication machinery, regulates DNA replication remains largely unknown. Here we show that histone H3 lysine 9 demethylase Kdm4d regulates DNA replication in eukaryotic cells. Depletion of Kdm4d results in defects in DNA replication, which can be rescued by the expression of H3K9M, a histone H3 mutant transgene that reverses the effect of Kdm4d on H3K9 methylation. Kdm4d interacts with replication proteins, and its recruitment to DNA replication origins depends on the two pre-replicative complex components (origin recognition complex [ORC] and minichromosome maintenance [MCM] complex)...
September 26, 2016: Nucleic Acids Research
Lichen Liu, Urbano Díaz, Raul Arenal, Giovanni Agostini, Patricia Concepción, Avelino Corma
Single metal atoms and metal clusters have attracted much attention thanks to their advantageous capabilities as heterogeneous catalysts. However, the generation of stable single atoms and clusters on a solid support is still challenging. Herein, we report a new strategy for the generation of single Pt atoms and Pt clusters with exceptionally high thermal stability, formed within purely siliceous MCM-22 during the growth of a two-dimensional zeolite into three dimensions. These subnanometric Pt species are stabilized by MCM-22, even after treatment in air up to 540 °C...
September 26, 2016: Nature Materials
Min-Ho Jin, Duckkyu Oh, Ju-Hyoung Park, Chun-Boo Lee, Sung-Wook Lee, Jong-Soo Park, Kwan-Young Lee, Dong-Wook Lee
For the application of formic acid as a liquid organic hydrogen carrier, development of efficient catalysts for dehydrogenation of formic acid is a challenging topic, and most studies have so far focused on the composition of metals and supports, the size effect of metal nanoparticles, and surface chemistry of supports. Another influential factor is highly desired to overcome the current limitation of heterogeneous catalysis for formic acid decomposition. Here, we first investigated the effect of support pore structure on formic acid decomposition performance at room temperature by using mesoporous silica materials with different pore structures such as KIE-6, MCM-41, and SBA-15, and achieved the excellent catalytic activity (TOF: 593 h(-1)) by only controlling the pore structure of mesoporous silica supports...
September 26, 2016: Scientific Reports
Paula Corell Escuin, Alfonso García-Bennett, Jose Vicente Ros-Lis, Angel Argüelles Foix, Ana Andrés
The ability of a number of mesoporous silica materials (SBA-15, SBA-3, and MCM-48) to immobilize polyphenol oxidase (PPO) at different pH has been tested. Pore size and volume are the structural characteristics with higher influence on the PPO immobilization. Mesoropous material SBA-15 adsorbs a larger quantity of PPO at pH 4.00 and offers an inhibition of enzymatic activity close the 50% in apple extracts.
February 15, 2017: Food Chemistry
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