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https://www.readbyqxmd.com/read/29158846/subcellular-localization-of-survivin-determines-its-function-in-cardiomyocytes
#1
Tien-Jui Tsang, Ying-Chang Hsueh, Erika I Wei, David J Lundy, Bill Cheng, You-Tzung Chen, Shoei-Shen Wang, Patrick C H Hsieh
Rationale: Reducing cardiomyocyte death and enhancing their proliferation after myocardial infarction is perhaps the single largest challenge for cardiac tissue regeneration. Survivin (SVV) is the smallest member of the inhibitor of apoptosis (IAP) family but plays two important roles; inhibiting caspase-9 activation in the intrinsic apoptosis pathway, and regulating microtubule dynamics and chromosome segregation during cell division. Genetic depletion of cardiac SVV leads to incomplete cardiomyocyte division and abnormal heart development...
2017: Theranostics
https://www.readbyqxmd.com/read/29155400/co-condensation-synthesis-of-well-defined-mesoporous-silica-nanoparticles-effect-of-surface-chemical-modification-on-plasmid-dna-condensation-and-transfection
#2
Mahdokht Mahmoodi, Abbas Behzad-Behbahani, Sadigheh Sharifzadeh, Samira Sadat Abolmaali, AliMohammad Tamaddon
Chemically modified mesoporous silica nanoparticles (MSNs) are of interest due to their chemical and thermal stability with adjustable morphology and porosity; therefore, it was aimed to develop and compare the MCM-41 MSNs functionalised with imidazole groups (MCM-41-Im) to unmodified (MCM-41-OH) and primary amine functionalised (MCM-41-NH2) MSNs for experimental gene delivery. The results show efficient transfection of the complexes of the plasmid and either MCM-41-NH2 or MCM-41-Im. Furthermore, following transfection of HeLa cells using MCM-41-Im, an enhanced GFP expression was achieved consistent with the noticeable DNase1 protection and endosomal escape properties of MCM-41-Im using carboxyfluorescein tracer...
December 2017: IET Nanobiotechnology
https://www.readbyqxmd.com/read/29151950/mcms-expression-in-lung-cancer-implication-of-prognostic-significance
#3
Yi-Zhen Liu, Bo-Shi Wang, Yan-Yi Jiang, Jian Cao, Jia-Jie Hao, Yu Zhang, Xin Xu, Yan Cai, Ming-Rong Wang
Minichromosome Maintenance (MCM) proteins play essential roles in various cancers. We previously reported that MCM7 could be a prognostic biomarker in non-small cell lung cancer (NSCLC). The purpose of current study is to explore roles of other MCM proteins in NSCLC and their correlation with clinico-pathologic parameters of NSCLC patients. We evaluated the expression of MCM2, MCM5 and MCM6 immunohistochemically in 571 primary NSCLC samples. High expression of MCM2, MCM5 and MCM6 was detected in 42.2%, 38.3% and 52...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/29150562/the-role-of-mhc-e-in-t-cell-immunity-is-conserved-among-humans-rhesus-macaques-and-cynomolgus-macaques
#4
Helen L Wu, Roger W Wiseman, Colette M Hughes, Gabriela M Webb, Shaheed A Abdulhaqq, Benjamin N Bimber, Katherine B Hammond, Jason S Reed, Lina Gao, Benjamin J Burwitz, Justin M Greene, Fidel Ferrer, Alfred W Legasse, Michael K Axthelm, Byung S Park, Simon Brackenridge, Nicholas J Maness, Andrew J McMichael, Louis J Picker, David H O'Connor, Scott G Hansen, Jonah B Sacha
MHC-E is a highly conserved nonclassical MHC class Ib molecule that predominantly binds and presents MHC class Ia leader sequence-derived peptides for NK cell regulation. However, MHC-E also binds pathogen-derived peptide Ags for presentation to CD8(+) T cells. Given this role in adaptive immunity and its highly monomorphic nature in the human population, HLA-E is an attractive target for novel vaccine and immunotherapeutic modalities. Development of HLA-E-targeted therapies will require a physiologically relevant animal model that recapitulates HLA-E-restricted T cell biology...
November 17, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29148972/rapid-dna-replication-origin-licensing-protects-stem-cell-pluripotency
#5
Jacob Peter Matson, Raluca Dumitru, Philip Coryell, Ryan M Baxley, Weili Chen, Kirk Twaroski, Beau R Webber, Jakub Tolar, Anja-Katrin Bielinsky, Jeremy E Purvis, Jeanette Gowen Cook
Complete and robust human genome duplication requires loading MCM helicase complexes at many DNA replication origins, an essential process termed origin licensing. Licensing is restricted to G1 phase of the cell cycle, but G1 length varies widely among cell types. Using quantitative single cell analyses we found that pluripotent stem cells with naturally short G1 phases load MCM much faster than their isogenic differentiated counterparts with long G1 phases. During the earliest stages of differentiation towards all lineages, MCM loading slows concurrently with G1 lengthening, revealing developmental control of MCM loading...
November 17, 2017: ELife
https://www.readbyqxmd.com/read/29145317/complete-remission-and-long-term-survival-of-a-patient-with-melanoma-metastases-treated-with-high-dose-fever-inducing-viscum-album-extract-a-case-report
#6
Paul G Werthmann, Alexander Hintze, Gunver S Kienle
INTRODUCTION: Metastatic malignant cutaneous melanoma (MCM)-a highly immunogenic cancer-typically has a poor prognosis. Viscum album extracts (VAEs) have strong immune-stimulating, apoptogenic, and cytotoxic effects. CASE PRESENTATION: A 66-year-old MCM patient with newly diagnosed lymph node metastases opted for sole VAE treatment. VAEs were initially applied subcutaneously, and then later in exceptionally high, fever-inducing doses, both intravenously and intralesionally...
November 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29144130/structural-phase-transitions-in-ptin2-at-high-pressure-a-theoretical-investigation
#7
P Modak, Ashok K Verma
A study of the bonding and electronic properties of intermetallics under pressure is crucial in the design and development of novel materials for useful applications. These properties are largely controlled by the underlying crystal structures. Here we investigated the high-pressure structural behavior of PtIn2 using crystal structure search calculations, which efficiently combine evolutionary algorithms and state-of-the-art density functional theory. Three new crystal structures, namely, Fe2B-type (I4/mcm, Z = 2), cotunnite-type (Pnma, Z = 4), and monoclinic (C2/m, Z = 2), are proposed at about 9...
November 16, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/29141658/thioredoxin-mitigates-radiation-induced-hematopoietic-stem-cell-injury-in-mice
#8
Pasupathi Sundaramoorthy, Qinhong Wang, Zhihong Zheng, Yiqun Jiao, Benny J Chen, Phuong L Doan, Nelson J Chao, Yubin Kang
BACKGROUND: Radiation exposure poses a significant threat to public health. Hematopoietic injury is one of the major manifestations of acute radiation sickness. Protection and/or mitigation of hematopoietic stem cells (HSCs) from radiation injury is an important goal in the development of medical countermeasure agents (MCM). We recently identified thioredoxin (TXN) as a novel molecule that has marked protective and proliferative effects on HSCs. In the current study, we investigated the effectiveness of TXN in rescuing mice from a lethal dose of total body radiation (TBI) and in enhancing hematopoietic reconstitution following a lethal dose of irradiation...
November 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29141316/-a-clinical-analysis-of-micafungin-treatment-of-pulmonary-invasive-fungal-infection-in-pediatric-patients-with-acute-leukemia-or-post-hematopoietic-stem-cells-transplantation
#9
K Huang, K Y Qiu, L L Deng, J P Fang, Y Li, H X Guo, D H Zhou
Objective: To investigate the efficacy and safety of micafungin (MCF) for pulmonary invasive fungal disease (PIFD) in pediatric patients with acute leukemia or post hematopoietic stem cells transplantation. Method: Twenty-five neutropenic PIFD children with acute leukemia or post hematopoietic stem cells transplantation in Sun Yat-sen Memorial Hospital of Sun Yat-sen University were selected from January 2012 to June 2015, including 12 males and 13 females, age range 2-15 (average 6.2±2.0) years. There were 12 cases of acute leukemia (AL) after chemotherapy, 4 cases of acute leukemia (AL) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and 9 cases of β-thalassemia major after allo-HSCT...
November 2, 2017: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/29137746/ultrasonically-tailored-chemically-engineered-and-qbd-enabled-fabrication-of-agomelatine-nanoemulsion-optimization-characterization-ex-vivo-permeation-and-stability-study
#10
Shakeeb Ahmed, Azka Gull, Mahtab Alam, Mohd Aqil, Yasmin Sultana
The objective of present study was to develop a nanoemulsion formulation of agomelatine (BCS class II drug) for the solubility enhancement. Capmul MCM, Tween 80 and PEG-400 were selected as oil, surfactant and co-surfactant respectively. The high energy ultrasonication method was used for the preparation of nanoemulsion. Three-factor three-level central composite design was employed to get the best formulation. The independent variables selected for the optimization were % oil, %Smix and sonication time (second)...
March 2018: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/29135113/minichromosome-maintenance-mcm-protein-4-overexpression-is-a-potential-prognostic-marker-for-laryngeal-squamous-cell-carcinoma
#11
Jianfeng Han, Meng Lian, Jugao Fang, Honggang Liu, Ru Wang, Jie Zhai, Yi Fan Yang, Ling Fen, Qian Shi, Hong Zhi Ma, Juan Sun, Zhihong Ma
PURPOSE: The minichromosomal maintenance (MCM) proteins are involved in the initiation and DNA replication. The role of MCM4 remains to be elucidated. The purpose of this study was to investigate the effects of MCM4 in laryngeal squamous cell carcinoma (LSCC) cell growth and apoptosis. METHODS: LSCC cell line UMSCC 5 was used in this study. The small interfering RNA (siRNA) of MCM 4 gene was used to identify the effects of MCM4 on the proliferation and apoptosis using methylimidazole tetrazolium (MTT) assay and flow-cytometry, respectively...
September 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/29102650/safety-and-feasibility-of-atrial-fibrillation-ablation-using-amigo-%C3%A2-system-versus-manual-approach-a-pilot-study
#12
Antonio Scarà, Luigi Sciarra, Ermenegildo De Ruvo, Alessio Borrelli, Domenico Grieco, Zefferino Palamà, Paolo Golia, Lucia De Luca, Marco Rebecchi, Leonardo Calò
BACKGROUND: The Amigo(®) Remote Catheter System is a relatively new robotic system for catheter navigation. This study compared feasibility and safety using Amigo (RCM) versus manual catheter manipulation (MCM) to treat paroxysmal atrial fibrillation (PAF). Contact force (CF) and force-time integral (FTI) values obtained during pulmonary vein isolation (PVI) ablation were compared. METHODS: Forty patients were randomly selected for either RCM (20) or MCM (20). All were studied with the Thermocool(®) SmartTouch(®) force-sensing catheter (STc)...
November 1, 2017: Indian Pacing and Electrophysiology Journal
https://www.readbyqxmd.com/read/29094948/in-vitro-dissolution-cellular-membrane-permeability-and-anti-inflammatory-response-of-resveratrol-encapsulated-mesoporous-silica-nanoparticles
#13
Estelle Juère, Justyna Florek, Meryem Bouchoucha, Siddharth Jambhrunkar, Kuan Yau Wong, Amirali Popat, Freddy Kleitz
Sizing drugs down to the sub-micron and nanometer scale using nanoparticles has been extensively used in pharmaceutical industries to overcome the poor aqueous solubility of potential therapeutic agents. Here, we report the encapsulation and release of resveratrol, a promising anti-inflammatory and anticancer nutraceutical, from the mesopores of MCM-48-type silica nanospheres of various particle sizes, i.e., 90, 150, and 300 nm. Furthermore, the influence of the carrier pore size on drug solubility was also evaluated (3...
November 2, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29079806/functionalization-of-microparticles-with-mineral-coatings-enhances-non-viral-transfection-of-primary-human-cells
#14
Andrew S Khalil, Xiaohua Yu, Angela W Xie, Gianluca Fontana, Jennifer M Umhoefer, Hunter J Johnson, Tracy A Hookway, Todd C McDevitt, William L Murphy
Gene delivery to primary human cells is a technology of critical interest to both life science research and therapeutic applications. However, poor efficiencies in gene transfer and undesirable safety profiles remain key limitations in advancing this technology. Here, we describe a materials-based approach whereby application of a bioresorbable mineral coating improves microparticle-based transfection of plasmid DNA lipoplexes in several primary human cell types. In the presence of these mineral-coated microparticles (MCMs), we observed up to 4-fold increases in transfection efficiency with simultaneous reductions in cytotoxicity...
October 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29078375/cryo-em-structure-of-mcm2-7-double-hexamer-on-dna-suggests-a-lagging-strand-dna-extrusion-model
#15
Yasunori Noguchi, Zuanning Yuan, Lin Bai, Sarah Schneider, Gongpu Zhao, Bruce Stillman, Christian Speck, Huilin Li
During replication initiation, the core component of the helicase-the Mcm2-7 hexamer-is loaded on origin DNA as a double hexamer (DH). The two ring-shaped hexamers are staggered, leading to a kinked axial channel. How the origin DNA interacts with the axial channel is not understood, but the interaction could provide key insights into Mcm2-7 function and regulation. Here, we report the cryo-EM structure of the Mcm2-7 DH on dsDNA and show that the DNA is zigzagged inside the central channel. Several of the Mcm subunit DNA-binding loops, such as the oligosaccharide-oligonucleotide loops, helix 2 insertion loops, and presensor 1 (PS1) loops, are well defined, and many of them interact extensively with the DNA...
November 7, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29078138/synthesis-characterization-and-nitrite-ion-sensing-performance-of-reclaimable-composite-samples-through-a-core-shell-structure
#16
Xiao Cui, Zhao Yuqing, Jiantao Cui, Qian Zheng, Wang Bo
The following paper reported and discussed a nitrite ion optical sensing platform based on a core-shell structure, using superamagnetic nanoparticles as the core, a silica molecular sieve MCM-41 as the shell and two rhodamine derivatives as probe, respectively. This superamagnetic core made this sensing platform reclaimable after finishing nitrite ion sensing procedure. This sensing platform was carefully characterized by means of electron microscopy images, porous structure analysis, magnetic response, IR spectra and thermal stability analysis...
October 18, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29077117/the-influence-of-particle-size-of-amino-functionalized-mcm-41-silicas-on-co2-adsorption
#17
G Gatti, C Vittoni, D Costenaro, G Paul, E Mangano, S Brandani, L Marchese, C Bisio
The CO2 adsorption properties of hybrid organic-inorganic MCM-41 silicas with different particle sizes are described here. Micrometric to nanometric MCM-41 silicas are functionalized by introducing amino groups via grafting of 3-[2-(2-aminoethyl)aminoethyl]aminopropyltrimethoxysilane (PAPTS). A combination of FTIR and SS-NMR spectroscopies is adopted to distinguish between physisorbed and chemisorbed CO2. A higher amount of CO2 is physisorbed in the nanometric sample because of a higher pore volume, whereas chemisorbed (carbamate and acid carbamic) species are more abundant in the micrometric sample...
November 8, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29070881/a-numerical-analysis-of-a-magnetocaloric-refrigerator-with-a-16-layer-regenerator
#18
Mingkan Zhang, Omar Abdelaziz, Ayyoub M Momen, Ahmad Abu-Heiba
A numerical analysis was conducted to study a room temperature magnetocaloric refrigerator with a 16-layer parallel plates active magnetic regenerator (AMR). Sixteen layers of LaFeMnSiH having different Curie temperatures were employed as magnetocaloric material (MCM) in the regenerator. Measured properties data was used. A transient one dimensional (1D) model was employed, in which a unique numerical method was developed to significantly accelerate the simulation speed of the multi-layer AMR system. As a result, the computation speed of a multi-layer AMR case was very close to the single-layer configuration...
October 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29068997/atypical-hemolytic-uremic-syndrome-induced-by-cblc-subtype-of-methylmalonic-academia-a-case-report-and-literature-review
#19
REVIEW
Minguang Chen, Jieqiu Zhuang, JianHuan Yang, Dexuan Wang, Qing Yang
RATIONALE: Methylmalonic acidemia (MMA) is a common organic acidemia, mainly due to methylmalonyl-CoA mutase (MCM) or its coenzyme cobalamin (VitB12) metabolic disorders. Cobalamin C (CblC) type is the most frequent inborn error of cobalamin metabolism; it can develop symptoms in childhood and often combine multisystem damage, which leads to methylmalonic acid, propionic acid, methyl citrate, and other metabolites abnormal accumulation, causing nerve, liver, kidney, bone marrow, and other organ damage...
October 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29057677/effect-of-different-polysorbates-on-development-of-self-microemulsifying-drug-delivery-systems-using-medium-chain-lipids
#20
Ankita Shah, Prajwal Thool, Komal Sorathiya, Hetal Prajapati, Damon Dalrymple, Abu T M Serajuddin
The primary objective of this study was to develop lipid-based self-microemulsifying drug delivery systems (SMEDDS) without using any organic cosolvents that would spontaneously form microemulsions upon dilution with water. Cosolvents were avoided to prevent possible precipitation of drug upon dilution and other stability issues. Different polysorbates, namely, Tween 20, Tween 40, Tween 60, and Tween 80, were used as surfactants, and Captex 355 EP/NF (glycerol tricaprylate/caprate) or its 1:1 mixture with Capmul MCM NF (glycerol monocaprylocaprate) were used as lipids...
October 23, 2017: Drug Development and Industrial Pharmacy
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