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https://www.readbyqxmd.com/read/28090355/ddx59-promotes-dna-replication-in-lung-adenocarcinoma
#1
Jin You, Xingshun Wang, Jiuling Wang, Baolei Yuan, Yandong Zhang
DEAD box proteins are multifunctional proteins involved in every aspect in RNA metabolism and have essential roles in many cellular activities. Despite their importance, many DEAD box proteins remain uncharacterized. In this report, we found DDX59 overexpressed in lung adenocarcinoma. DDX59 knockdown reduced cell proliferation, anchorage-independent cell growth, and caused reduction of tumor formation in immunocompromised mice. In multiple lung cancer cells, we found that DDX59 knockdown inhibits DNA synthesis; wild-type DDX59 but not helicase-defective mutant of DDX59 enhances DNA synthesis...
2017: Cell Death Discovery
https://www.readbyqxmd.com/read/28088360/hollow-porous-ionic-liquids-composite-polymers-based-solid-phase-extraction-coupled-online-with-high-performance-liquid-chromatography-for-selective-analysis-of-hydrophilic-hydroxybenzoic-acids-from-complex-samples
#2
Xingping Dai, Dongsheng Wang, Hui Li, Yanyi Chen, Zhicheng Gong, Haiyan Xiang, Shuyun Shi, Xiaoqing Chen
Polar and hydrophilic properties of hydroxybenzoic acids usually made them coelute with interferences in high performance liquid chromatography (HPLC) analysis. Then selective analysis of them was necessary. Herein, hollow porous ionic liquids composite polymers (PILs) based solid phase extraction (SPE) was firstly fabricated and coupled online with HPLC for selective analysis of hydroxybenzoic acids from complex matrices. Hollow porous PILs were firstly synthesized using Mobil Composition of Matter No. 48 (MCM-48) spheres as sacrificial support, 1-vinyl-3-methylimidazolium chloride (VMIM(+)Cl(-)) as monomer, and ethylene glycol dimethacrylate (EGDMA) as cross-linker...
January 9, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28081547/diagnosis-of-pancreaticobiliary-malignancy-by-detection-of-minichromosome-maintenance-protein-5-in-biliary-brush-cytology
#3
Margaret G Keane, Matthew T Huggett, Michael H Chapman, Gavin J Johnson, George J Webster, Douglas Thorburn, James Mackay, Stephen P Pereira
BACKGROUND: Biliary brush cytology is the standard method of evaluating biliary strictures, but is insensitive at detecting malignancy. In pancreaticobiliary cancer minichromosome maintenance replication proteins (MCM 2-7) are dysregulated in the biliary epithelium and MCM5 levels are elevated in bile samples. This study aimed to validate an immunocolorimetric ELISA assay for MCM5 as a pancreaticobiliary cancer biomarker in biliary brush samples. METHODS: Biliary brush specimens were collected prospectively at ERCP from patients with a biliary stricture...
January 12, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28077461/human-rif1-and-protein-phosphatase-1-stimulate-dna-replication-origin-licensing-but-suppress-origin-activation
#4
Shin-Ichiro Hiraga, Tony Ly, Javier Garzón, Zuzana Hořejší, Yoshi-Nobu Ohkubo, Akinori Endo, Chikashi Obuse, Simon J Boulton, Angus I Lamond, Anne D Donaldson
The human RIF1 protein controls DNA replication, but the molecular mechanism is largely unknown. Here, we demonstrate that human RIF1 negatively regulates DNA replication by forming a complex with protein phosphatase 1 (PP1) that limits phosphorylation-mediated activation of the MCM replicative helicase. We identify specific residues on four MCM helicase subunits that show hyperphosphorylation upon RIF1 depletion, with the regulatory N-terminal domain of MCM4 being particularly strongly affected. In addition to this role in limiting origin activation, we discover an unexpected new role for human RIF1-PP1 in mediating efficient origin licensing...
January 11, 2017: EMBO Reports
https://www.readbyqxmd.com/read/28070528/methylmalonic-acidemia-diagnosis-by-laboratory-methods
#5
Keyfi Fatemeh, Talebi Saeed, Varasteh Abdol-Reza
Methylmalonic acidemia (MMA) is usually caused by a deficiency of the enzyme methylmalonyl-CoA mutase (MCM), a defect in the transport or synthesis of its cofactor, adenosyl-cobalamin (cblA, cblB, cblC, cblF, cblD, and cblX), or deficiency of the enzyme methylmalonyl-CoA epimerase. A comprehensive diagnostic approach involves investigations of metabolites with tandem mass spectrometry, organic acid analysis with gas chromatography, enzymatic studies with fibroblast cell culture, and finally, mutation analysis...
October 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28069553/targeting-inflammasome-by-the-inhibition-of-caspase-1-activity-using-capped-mesoporous-silica-nanoparticles
#6
Alba García-Fernández, Guillermo García-Laínez, María Luisa Ferrándiz, Elena Aznar, Félix Sancenón, María José Alcaraz, José Ramón Murguía, María D Marcos, Ramón Martínez-Máñez, Ana M Costero, Mar Orzáez
Acute inflammation is a protective response of the body to harmful stimuli, such as pathogens or damaged cells. However, dysregulated inflammation can cause secondary damage and could thus contribute to the pathophysiology of many diseases. Inflammasomes, the macromolecular complexes responsible for caspase-1 activation, have emerged as key regulators of immune and inflammatory responses. Therefore, modulation of inflammasome activity has become an important therapeutic approach. Here we describe the design of a smart nanodevice that takes advantage of the passive targeting of nanoparticles to macrophages and enhances the therapeutic effect of caspase-1 inhibitor VX-765 in vivo...
January 6, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28054746/seed-assisted-synthesis-of-mww-type-zeolite-with-organic-structure-directing-agent-free-na-aluminosilicate-gel-system
#7
Yoshihiro Kamimura, Keiji Itabashi, Yoshihiro Kon, Akira Endo, Tatsuya Okubo
Seed-assisted synthesis of zeolite without using organic structure-directing agents (OSDAs) has enabled alternative routes to simple, environmentally friendly and low-cost production of industrially important zeolites. In this study, the successful seed-assisted synthesis of MCM-22 (MWW-type) zeolite with OSDA-free gel is reported for first time. MWW-type zeolite is obtained by the addition of as-synthesized MCM-22 seeds prepared with hexamethyleneimine (HMI) into OSDA-free Na-aluminosilicate gels. Based on the results of XRD, ICP-AES, NMR, N₂ physisorption and NH₃-TPD, product exhibited different features compared to those of the seeds...
January 5, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28054333/bmp-2-coated-mineral-coated-microparticles-improve-bone-repair-in-atrophic-non-unions
#8
M Orth, N J Kruse, B J Braun, C Scheuer, J H Holstein, A Khalil, X Yu, W L Murphy, T Pohlemann, M W Laschke, M D Menger
Atrophic non-unions are a major clinical problem. Mineral coated microparticles (MCM) are electrolyte-coated hydroxyapatite particles that have been shown in vitro to bind growth factors electrostatically and enable a tuneable sustained release. Herein, we studied whether MCM can be used in vivo to apply Bone Morphogenetic Protein-2 (BMP-2) to improve bone repair of atrophic non-unions. For this purpose, atrophic non-unions were induced in femurs of CD-1 mice (n = 48). Animals either received BMP-2-coated MCM (MCM + BMP; n = 16), uncoated MCM (MCM; n = 16) or no MCM (NONE; n = 16)...
January 2, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28024890/assessment-of-cell-viability-and-permeation-enhancement-in-presence-of-lipid-based-self-emulsifying-drug-delivery-systems-using-caco-2-cell-model-polysorbate-80-as-the-surfactant
#9
Pengli Bu, Yue Ji, Silpa Narayanan, Damon Dalrymple, Xingguo Cheng, Abu T M Serajuddin
PURPOSE: Lipid-based self-emulsifying drug delivery systems (SEDDS) are commonly used for solubilizing and enhancing oral bioavailability of poorly water-soluble drugs. However, their effects on viability of intestine epithelial cells and influence on membrane permeation are poorly understood. The present study was undertaken for safety assessment of lipid-based formulations containing medium-chain fatty acid esters as lipids and polysorbate 80 as the surfactant using the Caco-2 in vitro model...
December 24, 2016: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28018408/integrating-high-resolution-water-footprint-and-gis-for-promoting-water-efficiency-in-the-agricultural-sector-a-case-study-of-plantation-crops-in-the-jordan-valley
#10
Eliav Shtull-Trauring, Ido Aviani, Dror Avisar, Nirit Bernstein
Addressing the global challenges to water security requires a better understanding of humanity's use of water, especially the agricultural sector that accounts for 70% of global withdrawals. This study combined high resolution-data with a GIS system to analyze the impact of agricultural practices, crop type, and spatial factors such as drainage basins, climate, and soil type on the Water Footprint (WF) of agricultural crops. The area of the study, the northern Lower Jordan Valley, covers 1121 ha in which three main plantation crops are grown: banana (cultivated in open-fields or net-houses), avocado and palm-dates...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28004365/co-modified-mcm-41-as-an-effective-adsorbent-for-levofloxacin-removal-from-aqueous-solution-optimization-of-process-parameters-isotherm-and-thermodynamic-studies
#11
Ting Jin, Wenhua Yuan, Yujie Xue, Hong Wei, Chaoying Zhang, Kebin Li
Antibiotics are emerging contaminants due to their potential risks to human health and ecosystems. Poor biodegradability makes it necessary to develop effective physical-chemical methods to eliminate these contaminants from water. The cobalt-modified MCM-41 was prepared by a one-pot hydrothermal method and characterized by SAXRD, N2 adsorption-desorption, SEM, UV-Vis DR, and FTIR spectroscopy. The results revealed that the prepared 3% Co-MCM-41 possessed mesoporous structure with BET surface areas at around 898...
December 21, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28001354/mechanism-of-kinetically-controlled-capillary-condensation-in-nanopores-a-combined-experimental-and-monte-carlo-approach
#12
Tatsumasa Hiratsuka, Hideki Tanaka, Minoru T Miyahara
We find the rule of capillary condensation from the metastable state in nanoscale pores based on the transition state theory. The conventional thermodynamic theories cannot achieve it because the metastable capillary condensation inherently includes an activated process. We thus compute argon adsorption isotherms on cylindrical pore models and atomistic silica pore models mimicking the MCM-41 materials by the grand canonical Monte Carlo and the gauge cell Monte Carlo methods and evaluate the rate constant for the capillary condensation by the transition state theory...
December 27, 2016: ACS Nano
https://www.readbyqxmd.com/read/27991524/macrophages-enhance-migration-in-inflammatory-breast-cancer-cells-via-rhoc-gtpase-signaling
#13
Steven G Allen, Yu-Chih Chen, Julie M Madden, Chelsea L Fournier, Megan A Altemus, Ayse B Hiziroglu, Yu-Heng Cheng, Zhi Fen Wu, Liwei Bao, Joel A Yates, Euisik Yoon, Sofia D Merajver
Inflammatory breast cancer (IBC) is the most lethal form of breast cancer. All IBC patients have lymph node involvement and one-third of patients already have distant metastasis at diagnosis. This propensity for metastasis is a hallmark of IBC distinguishing it from less lethal non-inflammatory breast cancers (nIBC). Genetic profiling studies have been conducted to differentiate IBC from nIBC, but no IBC cancer-cell-specific gene signature has been identified. We hypothesized that a tumor-extrinsic factor, notably tumor-associated macrophages, promotes and contributes to IBC's extreme metastatic phenotype...
December 19, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27989548/dna-replication-and-inter-strand-crosslink-repair-symmetric-activation-of-dimeric-nanomachines
#14
Paolo Swuec, Alessandro Costa
Eukaryotic DNA replication initiation and the Fanconi anemia pathway of interstrand crosslink repair both revolve around the recruitment of a set of DNA-processing factors onto a dimeric protein complex, which functions as a loading platform (MCM and FANCI-FANCD2 respectively). Here we compare and contrast the two systems, identifying a set of unresolved mechanistic questions. How is the dimeric loading platform assembled on the DNA? How can equivalent covalent modification of both factors in a dimer be achieved? Are multicomponent DNA-interacting machines built symmetrically around their dimeric loading platform? Recent biochemical reconstitution studies are starting to shed light on these issues...
November 10, 2016: Biophysical Chemistry
https://www.readbyqxmd.com/read/27989442/how-the-eukaryotic-replisome-achieves-rapid-and-efficient-dna-replication
#15
Joseph T P Yeeles, Agnieska Janska, Anne Early, John F X Diffley
The eukaryotic replisome is a molecular machine that coordinates the Cdc45-MCM-GINS (CMG) replicative DNA helicase with DNA polymerases α, δ, and ε and other proteins to copy the leading- and lagging-strand templates at rates between 1 and 2 kb min(-1). We have now reconstituted this sophisticated machine with purified proteins, beginning with regulated CMG assembly and activation. We show that replisome-associated factors Mrc1 and Csm3/Tof1 are crucial for in vivo rates of replisome progression. Additionally, maximal rates only occur when DNA polymerase ε catalyzes leading-strand synthesis together with its processivity factor PCNA...
January 5, 2017: Molecular Cell
https://www.readbyqxmd.com/read/27989438/chromatin-controls-dna-replication-origin-selection-lagging-strand-synthesis-and-replication-fork-rates
#16
Christoph F Kurat, Joseph T P Yeeles, Harshil Patel, Anne Early, John F X Diffley
The integrity of eukaryotic genomes requires rapid and regulated chromatin replication. How this is accomplished is still poorly understood. Using purified yeast replication proteins and fully chromatinized templates, we have reconstituted this process in vitro. We show that chromatin enforces DNA replication origin specificity by preventing non-specific MCM helicase loading. Helicase activation occurs efficiently in the context of chromatin, but subsequent replisome progression requires the histone chaperone FACT (facilitates chromatin transcription)...
January 5, 2017: Molecular Cell
https://www.readbyqxmd.com/read/27986806/repair-of-meiotic-dna-breaks-and-homolog-pairing-in-mouse-meiosis-requires-a-minichromosome-maintenance-mcm-paralog
#17
Adrian J McNairn, Vera D Rinaldi, John C Schimenti
The mammalian Mcm-domain containing 2 (Mcmdc2) gene encodes a protein of unknown function that is homologous to the mini-chromosome maintenance family of DNA replication licensing and helicase factors. Drosophila melanogaster contains two separate genes, the "Mei-MCMs," that appear to have arisen from a single ancestral Mcmdc2 gene. The Mei-MCMs are involved in promoting meiotic crossovers by blocking the anti-crossover activity of BLM helicase, a function presumably performed by MSH4 and MSH5 in metazoans...
December 16, 2016: Genetics
https://www.readbyqxmd.com/read/27975914/rearranged-sesquiterpenes-produced-by-camarops-sp-an-endophytic-fungus-in-alibertia-macrophylla-rubiaceae
#18
J R Gubiani, C R Nogueira, McM Young, Pmp Ferreira, M O de Moraes, C Pessoa, V S Bolzani, A R Araujo
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27975888/two-new-compounds-produced-by-xylaria-sp-an-endophytic-fungus-from-casearia-sylvestris-flacourtiaceae
#19
G F Martins, C R Nogueira, G H Silva, McM Young, C O Pessoa, Djb Lima, Pmp Ferreira, V S Bolzani, A R Araujo
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27974207/dna-replication-origins-in-immunoglobulin-switch-regions-regulate-class-switch-recombination-in-an-r-loop-dependent-manner
#20
Eva-Maria Wiedemann, Mihaela Peycheva, Rushad Pavri
Class switch recombination (CSR) at the immunoglobulin heavy chain (IgH) locus generates antibody isotypes. CSR depends on double-strand breaks (DSBs) induced by activation-induced cytidine deaminase (AID). Although DSB formation and repair machineries are active in G1 phase, efficient CSR is dependent on cell proliferation and S phase entry; however, the underlying mechanisms are obscure. Here, we show that efficient CSR requires the replicative helicase, the Mcm complex. Mcm proteins are enriched at IgH switch regions during CSR, leading to assembly of facultative replication origins that require Mcm helicase function for productive CSR...
December 13, 2016: Cell Reports
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