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Armin Sadighi, Seyed Farshad Motevalizadeh, Morteza Hosseini, Ali Ramazani, Lena Gorgannezhad, Hamid Nadri, Behnaz Deiham, Mohammad Reza Ganjali, Abbas Shafiee, Mohammad Ali Faramarzi, Mehdi Khoobi
An enzyme immobilized on a mesoporous silica nanoparticle can serve as a multiple catalyst for the synthesis of industrially useful chemicals. In this work, MCM-41 nanoparticles were coated with polyethylenimine (MCM-41@PEI) and further modified by chelation of divalent metal ions (M = Co(2+), Cu(2+), or Pd(2+)) to produce metal-chelated silica nanoparticles (MCM-41@PEI-M). Thermomyces lanuginosa lipase (TLL) was immobilized onto MCM-41, MCM-41@PEI, and MCM-41@PEI-M by physical adsorption. Maximum immobilization yield and efficiency of 75 ± 3...
February 13, 2017: Applied Biochemistry and Biotechnology
Yuanliang Zhai, Erchao Cheng, Hao Wu, Ningning Li, Philip Yuk Kwong Yung, Ning Gao, Bik-Kwoon Tye
The minichromosome maintenance complex (MCM) hexameric complex (Mcm2-7) forms the core of the eukaryotic replicative helicase. During G1 phase, two Cdt1-Mcm2-7 heptamers are loaded onto each replication origin by the origin-recognition complex (ORC) and Cdc6 to form an inactive MCM double hexamer (DH), but the detailed loading mechanism remains unclear. Here we examine the structures of the yeast MCM hexamer and Cdt1-MCM heptamer from Saccharomyces cerevisiae. Both complexes form left-handed coil structures with a 10-15-Å gap between Mcm5 and Mcm2, and a central channel that is occluded by the C-terminal domain winged-helix motif of Mcm5...
February 13, 2017: Nature Structural & Molecular Biology
Leilei Luo, Yucang Liang, Egil Sev Erichsen, Reiner Anwander
Monodisperse and uniform high-quality MCM(Mobil Composition of Matter)-48-type CMSNs (Cubic Mesoporous Silica Nanoparticles) are readily prepared by simply optimizing the molar ratio of ethanol and surfactant in the system TEOS-CTAB-NaOH-H2O-EtOH (TEOS=tetraethyl orthosilicate, CTAB=cetyltrimethylammonium bromide, EtOH=ethanol). In the absence of ethanol only hexagonal mesoporous silica with ellipsoidal and spherical morphology are obtained. The presence of ethanol drives a mesophase transformation from hexagonal to mixed hexagonal/cubic, further to purely cubic, and finally to a mixed cubic/lamellar...
January 31, 2017: Journal of Colloid and Interface Science
Rasul Rakhshaei, Hassan Namazi
Lack of antibacterial activity, deficient water vapor and oxygen permeability, and insufficient mechanical properties are disadvantages of existing wound dressings. Hydrogels could absorb wound exudates due to their strong swelling ratio and give a cooling sensation and a wet environment. To overcome these shortcomings, flexible nanocomposite hydrogel films was prepared through combination of zinc oxide impregnated mesoporous silica (ZnO-MCM-41) as a nano drug carrier with carboxymethyl cellulose (CMC) hydrogel...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Junwei Zhao, Zhaoshuai He, Biao Li, Tanyu Cheng, Guohua Liu
Recently, the controlled drug delivery system has become a potential platform for biomedical application. Herein, we developed a pH and light-dual controlled cargo release system exhibiting AND logic based on MCM-41 mesoporous silica nanoparticles, which was surface modified using β-cyclodextrin (β-CD) with imine bond and azobenzene derivative. The complex of β-CD and azobenzene derivative effectively blocked the cargo delivery in pH=7.0 phosphate buffered saline (PBS) solution without 365nm UV light irradiation...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Ying Wang, Jiasheng Fang, John C Crittenden, Chanchan Shen
A novel solar-light-driven (SLD) Fenton catalyst was developed by reducing the ferrous-ion onto graphene oxide (GO) and forming reduced graphene oxide/α-FeOOH composites (RF) via in-situ induced self-assembly process. The RF was supported on several mesoporous supports (i.e., Al-MCM-41, MCM-41 and γ-Al2O3). The activity, stability and energy use for phenol oxidation were systematically studied for a wide pH range. Furthermore, the catalytic mechanism at acid and alkaline aqueous conditions was also elucidated...
January 23, 2017: Journal of Hazardous Materials
Sihai Yang, Xianhua Han, Yukako Tohsato, Koji Kyoda, Shuichi Onami, Ikuko Nishikawa, Yenwei Chen
In gene function analysis, it is arduous to identify gene function individually, and the way to screen out all involved genes according to a particular phenotype or disease usually shows us little information for a specific problem. We present a data-driven analysis system based on wild type (WT) embryos to study the concrete function of each gene associated with certain category of abnormal phenotypes. It can be applied to genes with very few RNAi embryos. Instead of presupposing the particular function of a gene, its function is confirmed by the statistical testing of built models...
February 8, 2017: Journal of Computational Biology: a Journal of Computational Molecular Cell Biology
Giuseppe Cannone, Silvia Visentin, Adeline Palud, Ghislaine Henneke, Laura Spagnolo
Cell division is a complex process that requires precise duplication of genetic material. Duplication is concerted by replisomes. The Minichromosome Maintenance (MCM) replicative helicase is a crucial component of replisomes. Eukaryotic and archaeal MCM proteins are highly conserved. In fact, archaeal MCMs are powerful tools for elucidating essential features of MCM function. However, while eukaryotic MCM2-7 is a heterocomplex made of different polypeptide chains, the MCM complexes of many Archaea form homohexamers from a single gene product...
February 8, 2017: Scientific Reports
Guang-Mao Li, Qiong Liu, Kui Wu, Zhi-Hua Yang, Shi-Lie Pan
In view of their inherent defects for commercial infrared nonlinear optical (IR NLO) materials, exploration for new IR NLO materials with excellent performance is an imperative and meaningful work. Herein, we report the successful design and synthesis of two metal-mixed chalcogenides containing divalent cations with d(10) electronic configuration: Na2CdGe2S6 and Na2CdGe2Se6. Both of them crystallize in the polar Cc space group and exhibit three-dimensional tunnel structures constructed by the CdQ4 tetrahedra and ∞[GeQ3]n chains with Na(+) located in the tunnels...
February 6, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
Surendra Kumar Jain, Roland J-M Pellenq, Keith E Gubbins, Xuan Peng
Realistic molecular models of silica-templated CMK-1, CMK-3, and CMK-5 carbon materials have been developed by using carbon rods and carbon pipes that were obtained by adsorbing carbon in a model MCM-41 pore. The interactions between the carbon atoms with the silica matrix were described using the PN-Traz potential, and the interaction between the carbon atoms was calculated by the reactive empirical bond order (REBO) potential. Carbon rods and pipes with different thicknesses were obtained by changing the silica-carbon interaction strength, the temperature, and the chemical potential of carbon vapor adsorption...
February 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
Kathryn M Spitler, Jessica M Ponce, Gavin Y Oudit, Duane D Hall, Chad E Grueter
The Mediator complex, a multisubunit nuclear complex, plays an integral role in regulating gene expression by acting as a bridge between transcription factors and RNA polymerase II. Genetic deletion of mediator subunit 1 (Med1) results in embryonic lethality, due in large part to impaired cardiac development. We first established that Med1 is dynamically expressed in cardiac development and disease, with marked upregulation of Med1 in both human and murine failing hearts. To determine if Med1 deficiency protects against cardiac stress, we generated two cardiac-specific Med1 knockout mouse models in which Med1 is conditionally deleted (Med1cKO mice) or inducibly deleted in adult mice (Med1cKO-MCM mice)...
February 3, 2017: American Journal of Physiology. Heart and Circulatory Physiology
Dengxin Fan, Ling Jia, Haiyan Xiang, Mijun Peng, Hui Li, Shuyun Shi
Hollow porous molecular imprinted polymers (HPMIPs) were prepared using caffeic acid (CA) as template, 4-vinylpyridine (4-VP) as functional monomer, and MCM-48 as sacrificial support. Large surface area (562.7m(2)/g) of HPMIPs resulted in high binding capacity (27.26mg/g) and fast kinetic binding (40min) in comparison with solid MIPs. Equilibrium data fitted well to Freundlich equation, and adsorption process with multi-diffusion mechanisms could be described by pseudo-second order model. Selectivity of HPMIPs was favorable...
June 1, 2017: Food Chemistry
Lotte M van Rijnberk, Suzanne E M van der Horst, Sander van den Heuvel, Suzan Ruijtenberg
Development, tissue homeostasis and tumor suppression depend critically on the correct regulation of cell division. Central in the cell division process is the decision whether to enter the next cell cycle and commit to going through the S and M phases, or to remain temporarily or permanently arrested. Cell cycle studies in genetic model systems could greatly benefit from visualizing cell cycle commitment in individual cells without the need of fixation. Here, we report the development and characterization of a reporter to monitor cell cycle entry in the nematode C...
2017: PloS One
Ha-Yeon Song, Hyo-Jin Choi, Hansaem Jeong, Dahye Choi, Dae-Hyuk Kim, Jung-Mi Kim
A double-stranded RNA (dsRNA) mycovirus was detected in malformed fruiting bodies of Pleurotus ostreatus strain ASI2792, one of bottle cultivated commercial strains of the edible oyster mushroom. The partial RNA-dependent RNA polymerase (RdRp) gene of the P. ostreatus ASI2792 mycovirus (PoV-ASI2792) was cloned, and a cDNA sequences alignment revealed that the sequence was identical to the RdRp gene of a known PoSV found in the P. ostreatus strain. To investigate the symptoms of PoV-ASI2792 infection by comparing the isogenic virus-free P...
December 2016: Mycobiology
Yan Yang, Liang Liu, Jianhua Zha, Ningyi Yuan
Two recyclable nitrite sensing composite samples were designed and constructed through a core-shell structure, with Fe3O4 nanoparticles as core, silica molecular sieve MCM-41 as shell and two rhodamine derivatives as chemosensors, respectively. These samples and their structure were identified with their electron microscopy images, N2 adsorption/desorption isotherms, magnetic response, IR spectra and thermogravimetric analysis. Their nitrite sensing behavior was discussed based on emission intensity quenching, their limit of detection was found as low as 1...
January 26, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Keith Dadson, Ludger Hauck, Zhenyue Hao, Daniela Grothe, Vivek Rao, Tak W Mak, Filio Billia
Cardiac homeostasis requires proper control of protein turnover. Protein degradation is principally controlled by the Ubiquitin-Proteasome System. Mule is an E3 ubiquitin ligase that regulates cellular growth, DNA repair and apoptosis to maintain normal tissue architecture. However, Mule's function in the heart has yet to be described. In a screen, we found reduced Mule expression in left ventricular samples from end-stage heart failure patients. Consequently, we generated conditional cardiac-specific Mule knockout (Mule (fl/fl(y));mcm) mice...
February 2, 2017: Scientific Reports
Kohshiro Fukushima, Mian Wang, Yoko Naito, Toshihiro Uchihashi, Yorika Kato, Satomi Mukai, Norikazu Yabuta, Hiroshi Nojima
Cyclin G-associated kinase (GAK) harbors a consensus phosphorylation motif (Y412) for c-Src; however, its physiological significance remains elusive. Here, we show that GAK is phosphorylated by c-Src not only at Y412 but also at Y1149. An anti-GAK-pY412 antibody recognized the shifted band of GAK during M phase. Immunofluorescence (IF) showed that GAK-pY412/pY1149 signals were present in the nucleus during interphase, translocated to chromosomes at prophase and prometaphase, moved to centrosomes at metaphase, and finally translocated to chromosomes at the end of telophase, when nuclear membrane formation was almost complete...
January 31, 2017: Cell Cycle
Richard Tyler Cimino, Piotr Kowalczyk, Peter I Ravikovitch, Alexander V Neimark
The heat of adsorption is one of the most important parameters characterizing energetic heterogeneity of the adsorbent surface. Heats of adsorption are either determined directly by calorimetry or calculated from adsorption isotherms measured at different temperatures using the thermodynamic Clausius-Clapeyron equation. Here, we present a method for calculating the isosteric heat of adsorption that requires as input only a single adsorption isotherm measured at one temperature. The proposed method is implemented with either non-local (NLDFT) or quenched solid (QSDFT) density functional theory models of adsorption that are currently widely used for calculating pore size distributions in various micro- and mesoporous solids...
January 30, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
Peng Cheng, Darren Svirskis, Sung Je Lee, Indrawati Oey, Hae-Soo Kwak, Guanyu Chen, Craig Bunt, Jingyuan Wen
Beta-carotene is a potent antioxidant for maintaining human health. However, its oral absorption is low due to poor aqueous solubility of less than 1 µg/ml. A microemulsion delivery system was designed to solubilize beta-carotene towards enhancing its oral bioavailability. From seven pseudo-ternary diagrams constructed, three systems were selected with large microemulsion areas suitable for oral administration and dilution in the predominately aqueous gastrointestinal fluids. Conductivity and rheology characterization were conducted along four dilution lines within the selected systems...
January 27, 2017: Pharmaceutical Development and Technology
Nuria Sánchez-Bastardo, Alberto Romero, Esther Alonso
The extraction/hydrolysis process of arabinoxylans from destarched wheat bran was studied in this work using different mesoporous silica supports and the corresponding RuCl3-based catalysts. The effects of temperature, time, catalyst supports and ruthenium catalysts were investigated and discussed in terms of the arabinoxylans extraction yield and their polymerization degree. Relatively high temperatures (180°C), short extraction times (10min) and RuCl3 supported on Al-MCM-48 led to a high amount of extracted arabinoxylans (78%) with a low molecular weight (9kDa)...
March 15, 2017: Carbohydrate Polymers
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