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Jordi Frigola, Jun He, Kerstin Kinkelin, Valerie E Pye, Ludovic Renault, Max E Douglas, Dirk Remus, Peter Cherepanov, Alessandro Costa, John F X Diffley
ORC, Cdc6 and Cdt1 act together to load hexameric MCM, the motor of the eukaryotic replicative helicase, into double hexamers at replication origins. Here we show that Cdt1 interacts with MCM subunits Mcm2, 4 and 6, which both destabilizes the Mcm2-5 interface and inhibits MCM ATPase activity. Using X-ray crystallography, we show that Cdt1 contains two winged-helix domains in the C-terminal half of the protein and a catalytically inactive dioxygenase-related N-terminal domain, which is important for MCM loading, but not for subsequent replication...
June 23, 2017: Nature Communications
Maider Virumbrales, Regino Saez-Puche, María José Torralvo, Veronica Blanco-Gutierrez
NiFe₂O₄ and ZnFe₂O₄ nanoparticles have been prepared encased in the MCM (Mobile Composition of Matter) type matrix. Their magnetic behavior has been studied and compared with that corresponding to particles of the same composition and of a similar size (prepared and embedded in amorphous silica or as bare particles). This study has allowed elucidation of the role exerted by the matrix and interparticle interactions in the magnetic behavior of each ferrite system. Thus, very different superparamagnetic behavior has been found in ferrite particles of similar size depending on the surrounding media...
June 22, 2017: Nanomaterials
Gabriel A Bedogni, Mauro D Acevedo, Federico Aguzín, Nora B Okulik, Cristina L Padró
In this paper, glycerol esterification with acetic acid was studied on several solid acid catalysts: Al2O3, Al-MCM-41, HPA/SiO2, HBEA, Amberlyst-15 and Amberlyst-36 with the aim of determining the reaction conditions and the nature of the surface acid sites required to produce selectively triacetylglycerol (triacetin). The acidity of the catalysts (nature, density and strength of acid sites) was characterized by temperature programmed desorption of NH3 and FTIR of adsorbed pyridine. Al2O3 (Lewis acidity) did not show any activity in the reaction...
June 22, 2017: Environmental Technology
Yang-Bo Guan, Dong-Rong Yang, Shao-Jun Nong, Jian Ni, Chun-Hui Hu, Jian Li, Jin Zhu, Yu-Xi Shan
Naturally occurring compounds are reported as effective candidates for prevention and treatment of various cancers. Breviscapine (BVP) is a mixture of flavonoid glycosides, derived from the Chinese herbs. Previous researches have indicated that BVP has comprehensive pharmacological functions. However, little is known about whether BVP has preventive effects on human prostate cancer. Here, we attempted to explore if BVP inhibits human prostate cancer in vitro and in vivo in a comprehensive manner. We found that BVP triggered cytotoxicity in prostate cancer cell lines dose-dependently...
June 16, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Zuzana Sekelova, Ondrej Polansky, Hana Stepanova, Radek Fedr, Marcela Faldynova, Ivan Rychlik, Lenka Vlasatikova
Lymphocytes represent the key antigen-specific leukocyte subpopulation. Despite their importance in mounting an immune response, an unbiased description of proteins expressed by chicken lymphocytes has not been presented. In this study we therefore intravenously infected chickens with Salmonella Enteritidis, sorted CD4, CD8 and γδ T-lymphocytes from the spleen by flow cytometry and determined the proteome of each population by LC-MS/MS. CD4 T-lymphocyte characteristic proteins included ubiquitin SUMO-like domain and BAR domain containing proteins...
June 16, 2017: Proteomics
Shuo Hao Li, Xiaodan Sun, Yang-Zhong Wang, Zhi-Peng Wang, Wen-Bo Gu, Wei-Miao Wang, He-Ming Yao, Ying Wang, Jian Hua Zhu
To meet the requirement of capturing tobacco specific nitrosamines (TSNA) in environment protection, a unique micro-environment was carefully created inside the channel of mesoporous silica MCM-41. In situ carbonization of template micelles at 923 K combined with the excess aluminum used in one-pot synthesis of MCM-41 are adopted to tailor the tortuosity of mecsoporous channels, while loaded metal oxides (5 wt.-%) and the Al-component in framework are employed to exert the necessary electrostatic interaction towards the target carcinogens TSNA in solution...
June 15, 2017: ACS Applied Materials & Interfaces
Yige Guo, Wenli Huang, Bin Chen, Ying Zhao, Dongfang Liu, Yu Sun, Bin Gong
In this study, nano zero valent iron (NZVI) modified MCM-41-zeolite A (Fe-MCM-41-A) composite as a novel adsorbent was prepared by precipitation method and applied for tetracycline (TC) removal from aqueous solution. The adsorbent was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS) and N2-BET analysis. Hysteresis loops indicated that the sample has a desirable magnetic property and can be separated quickly...
June 6, 2017: Journal of Hazardous Materials
Daniel Senfter, Erdogan Pekcan Erkan, Erdener Özer, Gerhard Jungwirth, Sibylle Madlener, Marcel Kool, Thomas Ströbel, Nurten Saydam, Okay Saydam
BACKGROUND: Overexpression of minichromosome maintenance (MCM) proteins 2, 3, and 7 is associated with migration and invasion in medulloblastoma (MB). However, expression profiling of all prereplication complex (pre-RC) has not been addressed in MBs. PROCEDURE: We performed mRNA expression profiling of a large set of pre-RC elements in cell lines and tumor tissues of MB. RNAi technology was employed for functional studies in MB cell lines. RESULTS: Our data showed that most of the pre-RC components are significantly overexpressed in MB...
June 9, 2017: Pediatric Blood & Cancer
Ožbej Zupančič, Julia Rohrer, Hung Thanh Lam, Julia Anita Grießinger, Andreas Bernkop-Schnürch
AIM: In this study SEDDS for oral delivery of opioid peptide dalargin were developed and characterized in vitro. METHODS: Dalargin lipophilicity was increased by O-esterification of tyrosine OH group, hydrophobic ion pairing or a combination thereof. Distribution coefficients (log D) of lipidized dalargin derivatives were determined. Then, dalargin was incorporated in chosen SEDDS, namely SEDDS-1, composed of 50% Capmul 907, 40% Cremophor EL and 10% propylene glycol and comparatively more lipophilic SEDDS-2 composed of 30% Captex 8000, 30% Capmul MCM, 30% Cremophor EL and 10% propylene glycol...
June 7, 2017: Drug Development and Industrial Pharmacy
Dianpeng Zheng, Sichao Ye, Xiuyun Wang, Yongjun Zhang, Daoyu Yan, Xiangsheng Cai, Weihong Gao, Hongbo Shan, Yang Gao, Juanjuan Chen, Zhiming Hu, Hongwei Li, Jinlong Li
MCM7, a subunit of mini-chromosome maintenance proteins (MCM) complex, plays an important role in initiating DNA replication during the G1 phase and extending DNA strands during the S phase. Here, we demonstrated that MCM7 is not only sustained but maintains association with chromatin during M phase. Remarkably, MCM7 siRNA can accelerate mitotic exit. MCM7 depletion leads to CDK1 inactivation and promotes subsequent cohesin/RAD21 cleavage, which eventually leads to sister chromatin segregation. Moreover, MCM7 is co-localized with tubulin in the mitotic cells and MCM7 depletion results in aberrant mitosis...
June 6, 2017: Scientific Reports
Laetitia Korchia, Vincent Lapinte, Christophe Travelet, Redouane Borsali, Jean-Jacques Robin, Cécile Bouilhac
A series of amphiphilic photo-responsive heterografted copolymers have been successfully synthesized. The random copolymers were composed of a methacrylate backbone, with various compositions of hydrophilic oligomeric 2-methyl-2-oxazoline side chains (OMOx) and hydrophobic long alkyl chains terminated by a coumarin moiety (Cm). Using dynamic (DLS) and static light scattering (SLS), and transmission electron microscopy (TEM), their self-assembling behavior was studied in water using the nanoprecipitation method...
June 6, 2017: Soft Matter
Chunxiang Ye, Dwayne E Heard, Lisa K Whalley
Photochemical cycling of Nitrogen oxides (NOx) produces tropospheric ozone (O3) and NOx is traditionally considered to be directly emitted. The inability of current global models to accurately calculate NOx levels, and concurrently, difficulties in performing direct NOx measurements in low-NOx regimes (several p.p.t.v. or several tens of p.p.t.v.) globally, introduce large uncertainty in the modelling of O3 formation. Here, we use the near-explicit Master Chemical Mechanism (MCM v3.2) within a 0-D box-model framework, to describe the chemistry of NOx and O3 in the remote marine boundary layer at Cape Verde...
June 5, 2017: Environmental Science & Technology
Chiara Giorio, Steven J Campbell, Maurizio Bruschi, Alexander T Archibald, Markus Kalberer
Ozonolysis of alkenes is a key reaction in the atmosphere, playing an important role in determining the oxidising capacity of the atmosphere and acting as a source of compounds that can contribute to local photochemical "smog". The reaction products of the initial step of alkene-ozonolysis are Criegee intermediates (CIs), which have for many decades eluded direct experimental detection because of their very short lifetime. We use an innovative experimental technique, stabilisation of CIs with spin traps and analysis with proton transfer reaction mass spectrometry, to measure the gas phase concentration of a series of CIs formed from the ozonolysis of a range of both biogenic and anthropogenic alkenes in flow tube experiments...
June 5, 2017: Faraday Discussions
Henrique Neves, Hang Fai Kwok
Cell division is a tightly-regulated process that involves the contribution of a large number of proteins. Before they are able to undergo mitosis, cells must first synthesize new DNA, effectively and accurately duplicating their genome. This occurs during what is called the S-phase and requires a fine control in order to avoid replication errors. The synthesis of new DNA takes place in the origin, specific locations in the genome where the double strands of DNA are unwound and separated, allowing for the binding of proteins and complexes that will build new strands of the genomic material, using the existing ones as molds, in what is referred to as semi-conservative process...
June 1, 2017: Biochimica et Biophysica Acta
Lingjun Kong, Huilong Yin, Li Yuan
We have recently reported that Rootletin prevents Cep68 from VHL-mediated proteasomal degradation to maintain centrosome cohesion, unveiling the first underlying mechanism of a linker protein required for maintenance of centrosome cohesion. The minichromosome maintenance (MCM) proteins 2-7 have long been noticed to localize to centrosomes, but their functions at the centrosome are presently unknown. Here, we show that MCM7 directly binds to the centrosomal linker protein Cep68 in vitro and complexes with Cep68 and VHL in vivo...
May 31, 2017: Biochemical and Biophysical Research Communications
Natalia I Cuello, Verónica R Elías, Silvia N Mendieta, Marcela Longhi, Mónica E Crivello, Marcos I Oliva, Griselda A Eimer
Mesoporous materials with superparamagnetic properties were successfully synthesized by two different methods: direct incorporation (DI) and wet impregnation (WI). The synthetized solids were evaluated as host of drugs for delivery systems and their physicochemical properties were characterized by XRD, ICP, N2 adsorption-desorption, spectroscopies of UV-Vis DR, FT-IR and their magnetic properties were measured. Indomethacin (IND) was incorporated into the materials and the kinetic of the release profiles was studied by applying the Pepas and Sahlin model...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Ivana Miletto, Geo Paul, Sthepanie Chapman, Giorgio Gatti, Leonardo Marchese, Robert Raja, Enrica Gianotti
Using a distinctive bottom-up approach, hierarchical SAPO-34 has been synthesized using CTAB encapsulated within ordered mesoporous silica (MCM-41) that serves as both the silicon source and mesoporogen. The structural and textural properties of the hierarchical SAPO-34 were contrasted against its microporous analogue, and the nature, strength and accessibility of the Brønsted acid sites were studied using a range of physicochemical characterization tools; notably probe-based FTIR and solid-state (SS) MAS NMR...
June 2, 2017: Chemistry: a European Journal
Chun Hai Wang, Brendan J Kennedy, André L Menezes de Oliveira, Julia Polt, Kevin Steven Knight
In this work we synthesized BaTaO2N and SrTaO2N using a two-step high-temperature solid-state reaction method and analysed the structural distortions, relative to the ideal cubic perovskite structure, according to group theory. From a complete distortion analysis/refinement using high-resolution neutron diffraction data in the temperature range 8 to 613 K, we identified tetragonal structures for BaTaO2N [P4/mmm (No. 123)] and SrTaO2N [I4/mcm (No. 140)]. In contrast to an anion-disordered cubic perovskite (Pm \overline{3}m No...
June 1, 2017: Acta Crystallographica Section B, Structural Science, Crystal Engineering and Materials
Michael D Rainey, Huong Quachthithu, David Gaboriau, Corrado Santocanale
The CDC7 kinase, by phosphorylating the MCM DNA helicase, is a key switch for DNA replication initiation. ATP competitive CDC7 inhibitors are being developed as potential anticancer agents; however how human cells respond to the selective pharmacological inhibition of this kinase is controversial and not understood. Here we have characterized the mode of action of the two widely used CDC7 inhibitors, PHA-767491 and XL-413, which have become important tool compounds to explore the kinase's cellular functions...
June 7, 2017: ACS Chemical Biology
Ata Amini, Ali Hesami
In this study, land use change and its effects on level and volume of groundwater were investigated. Using satellite images and field measurements, change in land uses was determined from 1998 to 2007. By analyzing the observation wells data and preparing the zoning maps in GIS, groundwater level fluctuations were assessed. Considering the area corresponding to these fluctuations, changes in aquifers volume were calculated. The rain gauge and synoptic stations data were used to calculate meteorological parameters and evapotranspiration...
June 2017: Environmental Monitoring and Assessment
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