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https://www.readbyqxmd.com/read/28632140/thermal-stability-and-rheological-properties-of-the-non-stick-caf1-biomaterial
#1
Yakup Ulusu, Gema Dura, Helen Waller, Matthew Benning, David Fulton, Jeremy Lakey, Daniel Thomas Peters
The ability to culture cells in three dimensions has many applications, from drug discovery to wound healing. 3D cell culture methods often require appropriate scaffolds that mimic the cellular environments of different tissue types. The choice of material from which these scaffolds are made is of paramount importance, as its properties will define the manner in which cells interact with the scaffold. Caf1 is a protein polymer that is secreted from its host organism, Yersinia pestis, to enable escape from phagocytosis...
June 20, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28591869/alternative-splicing-of-cnot7-diversifies-ccr4-not-functions
#2
Clément Chapat, Kamel Chettab, Pierre Simonet, Peng Wang, Pierre De La Grange, Muriel Le Romancer, Laura Corbo
The CCR4-associated factor CAF1, also called CNOT7, is a catalytic subunit of the CCR4-NOT complex, which has been implicated in all aspects of the mRNA life cycle, from mRNA synthesis in the nucleus to degradation in the cytoplasm. In human cells, alternative splicing of the CNOT7 gene yields a second CNOT7 transcript leading to the formation of a shorter protein, CNOT7 variant 2 (CNOT7v2). Biochemical characterization indicates that CNOT7v2 interacts with CCR4-NOT subunits, although it does not bind to BTG proteins...
June 7, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28559491/antagonistic-actions-of-two-human-pan3-isoforms-on-global-mrna-turnover
#3
Chyi-Ying A Chen, Yueqiang Zhang, Yu Xiang, Leng Han, Ann-Bin Shyu
Deadenylation is a fundamental process that regulates eukaryotic gene expression. Mammalian deadenylation exhibits biphasic kinetics, with the Pan2-Pan3 and Ccr4-Caf1 deadenylase complexes mediating the first and second phase, respectively; however, the significance of the biphasic nature of deadenylation in mRNA turnover remains unclear. In this study, we discovered that two distinct isoforms of human Pan3 display opposing properties necessary for coordinating the two phases of deadenylation. The shorter isoform (Pan3S) interacts more strongly with PABP than the longer isoform (Pan3L) does...
May 30, 2017: RNA
https://www.readbyqxmd.com/read/28374866/overexpression-of-chromatin-assembly-factor-1-p60-predicts-biological-behaviour-of-laryngeal-carcinomas
#4
M Mesolella, B Iorio, M Landi, M Cimmino, G Ilardi, M Iengo, M Mascolo
This study analysed the immunohistochemical expression of the CAF-1/p60 protein in laryngeal cancers. CAF-1/p60 assumes an independent discriminative and prognostic value in laryngeal neoplasms; the presence of this protein in carcinoma in situ compared with laryngeal precancerous and larynx infiltrating tumours. We assessed the immunohistochemical expression of CAF-1/p60 in 30 cases of moderate and/or severe dysplasia, 30 cases of carcinoma in situ and 30 cases of laryngeal squamous cell carcinoma (LSCCs)...
February 2017: Acta Otorhinolaryngologica Italica
https://www.readbyqxmd.com/read/28315525/insights-into-the-molecular-architecture-and-histone-h3-h4-deposition-mechanism-of-yeast-chromatin-assembly-factor-1
#5
Paul Victor Sauer, Jennifer Timm, Danni Liu, David Sitbon, Elisabetta Boeri-Erba, Christophe Velours, Norbert Mücke, Jörg Langowski, Françoise Ochsenbein, Geneviève Almouzni, Daniel Panne
How the very first step in nucleosome assembly, deposition of histone H3-H4 as tetramers or dimers on DNA, is accomplished remains largely unclear. Here, we report that yeast chromatin assembly factor 1 (CAF1), a conserved histone chaperone complex that deposits H3-H4 during DNA replication, binds a single H3-H4 heterodimer in solution. We identify a new DNA-binding domain in the large Cac1 subunit of CAF1, which is required for high-affinity DNA binding by the CAF1 three-subunit complex, and which is distinct from the previously described C-terminal winged-helix domain...
March 18, 2017: ELife
https://www.readbyqxmd.com/read/28271483/the-ccr4-not-complex-architecture-and-structural-insights
#6
Martine A Collart, Olesya O Panasenko
The Ccr4-Not complex is an essential multi-subunit protein complex that plays a fundamental role in eukaryotic mRNA metabolism and has a multitude of different roles that impact eukaryotic gene expression . It has a conserved core of three Not proteins, the Ccr4 protein, and two Ccr4 associated factors, Caf1 and Caf40. A fourth Not protein, Not4, is conserved, but is only a stable subunit of the complex in yeast. Certain subunits have been duplicated during evolution, with functional divergence, such as Not3 in yeast, and Ccr4 or Caf1 in human...
2017: Sub-cellular Biochemistry
https://www.readbyqxmd.com/read/28094282/evidence-of-neofunctionalization-after-the-duplication-of-the-highly-conserved-polycomb-group-gene-caf1-55-in-the-obscura-group-of-drosophila
#7
Juan M Calvo-Martín, Montserrat Papaceit, Carmen Segarra
Drosophila CAF1-55 protein is a subunit of the Polycomb repressive complex PRC2 and other protein complexes. It is a multifunctional and evolutionarily conserved protein that participates in nucleosome assembly and remodelling, as well as in the epigenetic regulation of a large set of target genes. Here, we describe and analyze the duplication of Caf1-55 in the obscura group of Drosophila. Paralogs exhibited a strong asymmetry in evolutionary rates, which suggests that they have evolved according to a neofunctionalization process...
January 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/27872192/the-chromatin-assembly-factor-complex-1-caf1-and-5-azacytidine-5-azac-affect-cell-motility-in-src-transformed-human-epithelial-cells
#8
Akinori Endo, Tony Ly, Raffaella Pippa, Dalila Bensaddek, Armel Nicolas, Angus I Lamond
Tumor invasion into surrounding stromal tissue is a hallmark of high grade, metastatic cancers. Oncogenic transformation of human epithelial cells in culture can be triggered by activation of v-Src kinase, resulting in increased cell motility, invasiveness, and tumorigenicity and provides a valuable model for studying how changes in gene expression cause cancer phenotypes. Here, we show that epithelial cells transformed by activated Src show increased levels of DNA methylation and that the methylation inhibitor 5-azacytidine (5-AzaC) potently blocks the increased cell motility and invasiveness induced by Src activation...
January 6, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27714489/novel-interaction-between-ccr4-and-caf1-in-rice-ccr4-not-deadenylase-complex
#9
Wei-Lun Chou, Yue-Lin Chung, Jhen-Cheng Fang, Chung-An Lu
Rice is an important crop in the world. However, little is known about rice mRNA deadenylation, which is an important regulation step of gene expression at the post-transcriptional level. The CCR4-NOT1 complex contains two key components, CCR4 and CAF1, which are the main cytoplasmic deadenylases in eukaryotic cells. In yeast and humans, CCR4 can interact with CAF1 via its N-terminal LRR domain. However, no CCR4 protein containing N-terminal LRR motifs have been found in plants. In this manuscript, we demonstrate a novel pattern of interaction between OsCCR4 and OsCAF1 in the rice CCR4-NOT complex, and that OsCAF1 acts as a bridge between OsCCR4 and OsNOT1 in this complex...
January 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/27635759/acetylation-dependent-control-of-global-poly-a-rna-degradation-by-cbp-p300-and-hdac1-2
#10
Sahil Sharma, Fabian Poetz, Marius Bruer, Thi Bach Nga Ly-Hartig, Johanna Schott, Bertrand Séraphin, Georg Stoecklin
Acetylation of histones and transcription-related factors is known to exert epigenetic and transcriptional control of gene expression. Here we report that histone acetyltransferases (HATs) and histone deacetylases (HDACs) also regulate gene expression at the posttranscriptional level by controlling poly(A) RNA stability. Inhibition of HDAC1 and HDAC2 induces massive and widespread degradation of normally stable poly(A) RNA in mammalian and Drosophila cells. Acetylation-induced RNA decay depends on the HATs p300 and CBP, which acetylate the exoribonuclease CAF1a, a catalytic subunit of the CCR4-CAF1-NOT deadenlyase complex and thereby contribute to accelerating poly(A) RNA degradation...
September 15, 2016: Molecular Cell
https://www.readbyqxmd.com/read/27609860/optimization-of-light-harvesting-pigment-improves-photosynthetic-efficiency
#11
Honglei Jin, Mengshu Li, Sujuan Duan, Mei Fu, Xiaoxiao Dong, Bing Liu, Dongru Feng, Jinfa Wang, Hong-Bin Wang
Maximizing light capture by light harvesting pigment optimization represents an attractive but challenging strategy to improve photosynthetic efficiency. Here, we report that loss of a previously uncharacterized gene HIGH PHOTOSYNTHETIC EFFICIENCY 1 (HPE1) optimizes light harvesting pigments leading to improved photosynthetic efficiency and biomass production. Arabidopsis hpe1 mutants show faster electron transport and increased contents of carbohydrates. HPE1 encodes a chloroplast protein containing a RNA recognition motif which directly associates with and regulates the splicing of target RNAs of plastid genes...
September 8, 2016: Plant Physiology
https://www.readbyqxmd.com/read/27606595/yersinia-pestis-caf1-protein-effect-of-sequence-polymorphism-on-intrinsic-disorder-propensity-serological-cross-reactivity-and-cross-protectivity-of-isoforms
#12
Pavel Kh Kopylov, Mikhail E Platonov, Vitaly G Ablamunits, Tat'yana I Kombarova, Sergey A Ivanov, Lidiya A Kadnikova, Aleksey N Somov, Svetlana V Dentovskaya, Vladimir N Uversky, Andrey P Anisimov
Yersinia pestis Caf1 is a multifunctional protein responsible for antiphagocytic activity and is a key protective antigen. It is generally conserved between globally distributed Y. pestis strains, but Y. pestis subsp. microtus biovar caucasica strains circulating within populations of common voles in Georgia and Armenia were reported to carry a single substitution of alanine to serine. We investigated polymorphism of the Caf1 sequences among other Y. pestis subsp. microtus strains, which have a limited virulence in guinea pigs and in humans...
2016: PloS One
https://www.readbyqxmd.com/read/27605379/the-btg4-and-caf1-complex-prevents-the-spontaneous-activation-of-eggs-by-deadenylating-maternal-mrnas
#13
Michał Pasternak, Sybille Pfender, Balaji Santhanam, Melina Schuh
Once every menstrual cycle, eggs are ovulated into the oviduct where they await fertilization. The ovulated eggs are arrested in metaphase of the second meiotic division, and only complete meiosis upon fertilization. It is crucial that the maintenance of metaphase arrest is tightly controlled, because the spontaneous activation of the egg would preclude the development of a viable embryo (Zhang et al. 2015 J. Genet. Genomics 42, 477-485. (doi:10.1016/j.jgg.2015.07.004); Combelles et al. 2011 Hum. Reprod. 26, 545-552...
September 2016: Open Biology
https://www.readbyqxmd.com/read/27558897/ythdf2-destabilizes-m-6-a-containing-rna-through-direct-recruitment-of-the-ccr4-not-deadenylase-complex
#14
Hao Du, Ya Zhao, Jinqiu He, Yao Zhang, Hairui Xi, Mofang Liu, Jinbiao Ma, Ligang Wu
Methylation at the N6 position of adenosine (m(6)A) is the most abundant RNA modification within protein-coding and long noncoding RNAs in eukaryotes and is a reversible process with important biological functions. YT521-B homology domain family (YTHDF) proteins are the readers of m(6)A, the binding of which results in the alteration of the translation efficiency and stability of m(6)A-containing RNAs. However, the mechanism by which YTHDF proteins cause the degradation of m(6)A-containing RNAs is poorly understood...
August 25, 2016: Nature Communications
https://www.readbyqxmd.com/read/27484313/ccr4-and-caf1-deadenylases-have-an-intrinsic-activity-to-remove-the-post-poly-a-sequence
#15
Sho Niinuma, Takashi Fukaya, Yukihide Tomari
MicroRNAs (miRNAs) recruit the CCR4-NOT complex, which contains two deadenylases, CCR4 and CAF1, to promote shortening of the poly(A) tail. Although both CCR4 and CAF1 generally have a strong preference for poly(A) RNA substrates, it has been reported from yeast to humans that they can also remove non-A residues in vitro to various degrees. However, it remains unknown how CCR4 and CAF1 remove non-A sequences. Herein we show that Drosophila miRNAs can promote the removal of 3'-terminal non-A residues in an exonucleolytic manner, but only if an upstream poly(A) sequence exists...
October 2016: RNA
https://www.readbyqxmd.com/read/27458447/a-recombinant-trivalent-fusion-protein-f1-lcrv-hsp70-ii-augments-humoral-and-cellular-immune-responses-and-imparts-full-protection-against-yersinia-pestis
#16
Shailendra K Verma, Lalit Batra, Urmil Tuteja
Plague is one of the most dangerous infections in humans caused by Yersinia pestis, a Gram-negative bacterium. Despite of an overwhelming research success, no ideal vaccine against plague is available yet. It is well established that F1/LcrV based vaccine requires a strong cellular immune response for complete protection against plague. In our earlier study, we demonstrated that HSP70(II) of Mycobacterium tuberculosis modulates the humoral and cellular immunity of F1/LcrV vaccine candidates individually as well as in combinations in a mouse model...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27426699/low-and-moderate-doses-of-caffeine-late-in-exercise-improve-performance-in-trained-cyclists
#17
RANDOMIZED CONTROLLED TRIAL
Jason L Talanian, Lawrence L Spriet
The aim of the present study was to assess if low and moderate doses of caffeine delivered in a carbohydrate-electrolyte solution (CES) late in exercise improved time-trial (TT) performance. Fifteen (11 male, 4 female) cyclists (age, 22.5 ± 0.9 years; body mass, 69.3 ± 2.6 kg; peak oxygen consumption, 64.6 ± 1.9 mL·min(-1)·kg(-1)) completed 4 double-blinded randomized trials. Subjects completed 120 min of cycling at ∼60% peak oxygen consumption with 5 interspersed 120-s intervals at ∼82% peak oxygen consumption, immediately followed by 40-s intervals at 50 W...
August 2016: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/27358572/caf1-knockout-mice-are-more-susceptive-to-lipopolysaccharide-induced-acute-lung-injury
#18
Jia-Xin Shi, Jia-Shu Li, Rong Hu, Xiao-Min Li, Hong Wang
The carbon catabolite repressor protein 4 (CCR4)-negative on TATA (NOT) complex includes multiple subunits and is conserved in the eukaryotic cells. The CCR4-NOT complex can regulate gene expression at different levels. Two subunits of the CCR4-NOT complex, CCR4 and CCR4-associated factor 1 (CAF1), possess deadenylase activity. In yeast, the deadenylase activity is mainly provided by the CCR4 subunit; however, the deadenylase activity is provided by both CCR4 and CAF1 in other eukaryotes. A previous study reported that CAF1 but not CCR4 is required for the decay of a reporter mRNA with AU-rich elements...
2016: Journal of Inflammation Research
https://www.readbyqxmd.com/read/27170642/a-replication-defective-human-type-5-adenovirus-based-trivalent-vaccine-confers-complete-protection-against-plague-in-mice-and-nonhuman-primates
#19
Jian Sha, Michelle L Kirtley, Curtis Klages, Tatiana E Erova, Maxim Telepnev, Duraisamy Ponnusamy, Eric C Fitts, Wallace B Baze, Satheesh K Sivasubramani, William S Lawrence, Igor Patrikeev, Jennifer E Peel, Jourdan A Andersson, Elena V Kozlova, Bethany L Tiner, Johnny W Peterson, David McWilliams, Snehal Patel, Eric Rothe, Vladimir L Motin, Ashok K Chopra
Currently, no plague vaccine exists in the United States for human use. The capsular antigen (Caf1 or F1) and two type 3 secretion system (T3SS) components, the low-calcium-response V antigen (LcrV) and the needle protein YscF, represent protective antigens of Yersinia pestis We used a replication-defective human type 5 adenovirus (Ad5) vector and constructed recombinant monovalent and trivalent vaccines (rAd5-LcrV and rAd5-YFV) that expressed either the codon-optimized lcrV or the fusion gene designated YFV (consisting of ycsF, caf1, and lcrV)...
July 2016: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/27106059/establishment-of-a-promoter-based-chromatin-architecture-on-recently-replicated-dna-can-accommodate-variable-inter-nucleosome-spacing
#20
Ross T Fennessy, Tom Owen-Hughes
Nucleosomes, the fundamental subunits of eukaryotic chromatin, are organized with respect to transcriptional start sites. A major challenge to the persistence of this organization is the disassembly of nucleosomes during DNA replication. Here, we use complimentary approaches to map the locations of nucleosomes on recently replicated DNA. We find that nucleosomes are substantially realigned with promoters during the minutes following DNA replication. As a result, the nucleosomal landscape is largely re-established before newly replicated chromosomes are partitioned into daughter cells and can serve as a platform for the re-establishment of gene expression programmes...
September 6, 2016: Nucleic Acids Research
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