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https://www.readbyqxmd.com/read/29043951/verrucosispora-endophytica-sp-nov-isolated-from-the-root-of-wild-orchid-grosourdya-appendiculata-blume-rchb-f
#1
Wipaporn Ngaemthao, Tawanmol Pujchakarn, Suwanee Chunhametha, Chanwit Suriyachadkun
A novel endophytic filamentous bacterium strain A-T 7972(T), was isolated from wild orchid Grosourdya appendiculata (Blume) Rchb.f. collected in Thailand. The bacterium developed single non-motile spores with warty surface on substrate mycelia. The taxonomic position was described using a polyphasic approach. The 16S rRNA gene sequence and phylogenetic analysis indicated that strain A-T 7972(T) belonged to the genus Verrucosispora and shared the highest sequence similarity with V. lutea YIM 013(T) (98.71 %) and V...
October 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29039546/antiproliferative-effects-of-tsa-pxd%C3%A2-101-and-ms%C3%A2-275-in-a2780-and-mcf7-cells-acetylated-histone-h4-and-acetylated-tubulin-as-markers-for-hdaci-potency-and-selectivity
#2
Vasilis P Androutsopoulos, Demetrios A Spandidos
Inhibition of histone deacetylase enzymes (HDACs) has been well documented as an attractive target for the development of chemotherapeutic drugs. The present study investigated the effects of two prototype hydroxamic acid HDAC inhibitors, namely Trichostatin A (TSA) and Belinostat (PXD‑101) and the benzamide Entinostat (MS‑275) in A2780 ovarian carcinoma and MCF7 breast adenocarcinoma cells. The three HDACi inhibited the proliferation of A2780 and MCF7 cells at comparable levels, below the µM range. Enzyme inhibition assays in a cell‑free system showed that TSA was the most potent inhibitor of total HDAC enzyme activity followed by PXD‑101 and MS‑275...
October 6, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29039274/different-escherichia-coli-b2-st131-clades-b-and-c-producing-extended-spectrum-%C3%AE-lactamases-esbl-colonizing-residents-of-portuguese-nursing-homes
#3
C Rodrigues, E Machado, S Fernandes, L Peixe, Â Novais
ESBL-producing Enterobacteriaceae and particularly Escherichia coli ST131 isolates producing CTX-M enzymes are commonly found colonizing the intestine of nursing home (NH) residents, but ST131 subclonal structure has been scarcely explored in this vulnerable population. Our goal was to perform a pilot study to assess the faecal carriage rate and epidemiological features of ESBL- and/or carbapenemase-producing Enterobacteriaceae (ESBL-E and CPE, respectively) among NH residents. For this purpose, faecal samples from residents at 4 different NHs in the North of Portugal (representing 9·5% of the residents' population, July 2014) were screened for ESBL-E and/or CPE by phenotypic and genotypic methods...
October 17, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/29038501/effects-of-charge-modifying-mutations-in-histone-h2a-%C3%AE-3-domain-on-nucleosome-stability-assessed-by-single-pair-fret-and-md-simulations
#4
Kathrin Lehmann, Ruihan Zhang, Nathalie Schwarz, Alexander Gansen, Norbert Mücke, Jörg Langowski, Katalin Toth
Nucleosomes are important for chromatin compaction and gene regulation; their integrity depends crucially on the structural properties of the histone tails. Recent all-atom molecular dynamics simulations revealed that removal of the N-terminal tails of histone H3, known to destabilize nucleosomes, causes a rearrangement of two arginines of histone H2A, namely R81 and R88 by altering the electrostatic environment of the H2A α3 domain. Whether this rearrangement is the cause or the effect of decreased stability, is unclear...
October 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29035377/nme4-nucleoside-diphosphate-kinase-d-in-cardiolipin-signaling-and-mitophagy
#5
Uwe Schlattner, Malgorzata Tokarska-Schlattner, Richard M Epand, Mathieu Boissan, Marie-Lise Lacombe, Valerian E Kagan
Mitophagy is an emerging paradigm for mitochondrial quality control and cell homeostasis. Dysregulation of mitophagy can lead to human pathologies such as neurodegenerative disorders and contributes to the aging process. Complex protein signaling cascades have been described that regulate mitophagy. We have identified a novel lipid signaling pathway that involves the phospholipid cardiolipin (CL). CL is synthesized and normally confined at the inner mitochondrial membrane. However, upon a mitophagic trigger, ie, collapse of the inner membrane potential, CL is rapidly externalized to the mitochondrial surface with the assistance of the hexameric nucleoside diphosphate kinase D (NME4, NDPK-D, or NM23-H4)...
October 16, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29035199/structure-based-nuclear-import-mechanism-of-histones-h3-and-h4-mediated-by-kap123
#6
Sojin An, Jungmin Yoon, Hanseong Kim, Ji-Joon Song, Uhn-Soo Cho
Kap123, a major karyopherin protein of budding yeast, recognizes the nuclear localization signals (NLSs) of cytoplasmic histones H3 and H4 and translocates them into the nucleus during DNA replication. Mechanistic questions include H3- and H4-NLS redundancy toward Kap123 and the role of the conserved diacetylation of cytoplasmic H4 (K5ac and K12ac) in Kap123-mediated histone nuclear translocation. Here, we report crystal structures of full-length Kluyveromyces lactis Kap123 alone and in complex with H3- and H4-NLSs...
October 16, 2017: ELife
https://www.readbyqxmd.com/read/29034849/streptomyces-amphotericinicus-sp-nov-an-amphotericin-producing-actinomycete-isolated-from-the-head-of-an-ant-camponotus-japonicus-mayr
#7
Tingting Cao, Shan Mu, Chang Lu, Shanshan Zhao, Dongmei Li, Kai Yan, Wensheng Xiang, Chongxi Liu
A novel actinomycete, designated strain 1H-SSA8(T), was isolated from the head of an ant (Camponotus japonicus Mayr) and was found to produce amphotericin. A polyphasic approach was employed to determine the status of strain 1H-SSA8(T). Morphological and chemotaxonomic characteristics were consistent with those of members of the genus Streptomyces. The menaquinones detected were MK-9(H6), MK-9(H8) and MK-9(H4). The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and phosphatidylinositol mannoside...
October 16, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29034626/-sequence-analysis-and-expression-patterns-of-histone-h2a-variants-in-phytophthora-infestans
#8
Xiaowen Wang, Liyun Guo
In eukaryotic cells, DNA is packaged inside the nucleus together with histones to form nucleosomes. Each histone molecule contains two of each core histone subunits H2A, H2B, H3 and H4. Among core histones, the H2A family is of great interest due to the high diversity of specialized variants. These variants have shown important role in critical cellular processes. Epigenetic mechanism in oomycetes is barely known. Phytophthora infestans is a severe pathogen and a model species in oomycetes. In this study, we studied the sequence and expression pattern of H2A variants of P...
November 25, 2016: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29030587/natd-promotes-lung-cancer-progression-by-preventing-histone-h4-serine-phosphorylation-to-activate-slug-expression
#9
Junyi Ju, Aiping Chen, Yexuan Deng, Ming Liu, Ying Wang, Yadong Wang, Min Nie, Chao Wang, Hong Ding, Bing Yao, Tao Gui, Xinyu Li, Zhen Xu, Chi Ma, Yong Song, Marc Kvansakul, Ke Zen, Chen-Yu Zhang, Cheng Luo, Ming Fang, David C S Huang, C David Allis, Renxiang Tan, Changjiang Kathy Zeng, Jiwu Wei, Quan Zhao
N-α-acetyltransferase D (NatD) mediates N-α-terminal acetylation (Nt-acetylation) of histone H4 known to be involved in cell growth. Here we report that NatD promotes the migratory and invasive capabilities of lung cancer cells in vitro and in vivo. Depletion of NatD suppresses the epithelial-to-mesenchymal transition (EMT) of lung cancer cells by directly repressing the expression of transcription factor Slug, a key regulator of EMT. We found that Nt-acetylation of histone H4 antagonizes histone H4 serine 1 phosphorylation (H4S1ph), and that downregulation of Nt-acetylation of histone H4 facilitates CK2α binding to histone H4 in lung cancer cells, resulting in increased H4S1ph and epigenetic reprogramming to suppress Slug transcription to inhibit EMT...
October 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/29030065/diuretics-versus-volume-expansion-in-acute-submassive-pulmonary-embolism
#10
Elie Dan Schouver, Olivier Chiche, Priscille Bouvier, Denis Doyen, Pierre Cerboni, Pamela Moceri, Emile Ferrari
BACKGROUND: The benefit of volume expansion (VE) in submassive pulmonary embolism (PE) with right ventricular (RV) dysfunction is unclear. AIM: To compare the effects of diuretic treatment versus VE in patients hospitalized for PE with RV dysfunction. METHODS: We prospectively included 46 consecutive patients with submassive PE treated on admission with a 40mg bolus of furosemide (D group, n=24) or 500mL of saline infusion (VE group, n=22)...
October 10, 2017: Archives of Cardiovascular Diseases
https://www.readbyqxmd.com/read/29022551/lentzea-pudingi-sp-nov-isolated-from-a-weathered-limestone-sample-in-a-karst-area
#11
Chengliang Cao, Bo Yuan, Sheng Qin, Jihong Jiang, Faxiang Tao, Bin Lian
A novel Gram-stain-positive, aerobic bacterium, designated strain DHS C021(T), was isolated from a limestone sample collected from the Puding Karst Ecosystem Research Station of Guizhou Province, southwest China. This strain developed branched vegetative mycelia, and its aerial mycelia fragmented into rod-shaped spores. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the whole-cell sugars comprised galactose, ribose and mannose. The respiratory quinone was identified as menaquinone MK-9(H4)...
October 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29022545/nocardioides-litorisoli-sp-nov-isolated-from-lakeside-soil
#12
Yu Wang, Ding Xu, Anna Luo, Gejiao Wang, Shixue Zheng
A Gram-stain-positive, rod-shaped, non-spore-forming bacterium, designated as x-2(T), was isolated from lakeside soil of Sayram in the Xinjiang Uygur Autonomous Region, PR China. On the basis of 16S rRNA gene sequences, strain x-2(T) belongs to the genus Nocardioides in the family Nocardioidaceae, being most closely related to Nocardioides panacisoli Gsoil 346(T) (97.36 % 16S rRNA gene sequence similarity). Strain x-2(T) was characterized chemotaxonomically and found to have ll-diaminopimelic acid in the cell-wall peptidoglycan, phosphatidylglycerol, diphosphatidylglycerol, glycolipids and another three unknown phospholipids as the major polar lipids, MK-8(H4) as the predominant menaquinone and C18 : 1ω9c, iso-C16 : 0, C17 : 1ω8c and C16 : 0 as the major fatty acids...
October 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29019976/nucleosome-chd1-structure-and-implications-for-chromatin-remodelling
#13
Lucas Farnung, Seychelle M Vos, Christoph Wigge, Patrick Cramer
Chromatin-remodelling factors change nucleosome positioning and facilitate DNA transcription, replication, and repair. The conserved remodelling factor chromodomain-helicase-DNA binding protein 1(Chd1) can shift nucleosomes and induce regular nucleosome spacing. Chd1 is required for the passage of RNA polymerase IIthrough nucleosomes and for cellular pluripotency. Chd1 contains the DNA-binding domains SANT and SLIDE, a bilobal motor domain that hydrolyses ATP, and a regulatory double chromodomain. Here we report the cryo-electron microscopy structure of Chd1 from the yeast Saccharomyces cerevisiae bound to a nucleosome at a resolution of 4...
October 11, 2017: Nature
https://www.readbyqxmd.com/read/29018219/spatially-restricted-loading-of-brd2-at-dna-double-strand-breaks-protects-h4-acetylation-domains-and-promotes-dna-repair
#14
Ozge Gursoy-Yuzugullu, Chelsea Carman, Brendan D Price
The n-terminal tail of histone H4 recruits repair proteins, including 53BP1, to DNA double-strand breaks (DSB) and undergoes dynamic acetylation during DSB repair. However, how H4 acetylation (H4Ac) recruits repair proteins and reorganizes chromatin during DNA repair is unclear. Here, we show that the bromodomain protein BRD2 is recruited to DSBs. This recruitment requires binding of BRD2's tandem bromodomains to H4Ac, which is generated at DSBs by the Tip60/KAT5 acetyltransferase. Binding of BRD2 to H4Ac protects the underlying acetylated chromatin from attack by histone deacetylases and allows acetylation to spread along the flanking chromatin...
October 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29017950/the-synergistic-effects-of-valproic-acid-and-fluvastatin-on-apoptosis-induction-in-glioblastoma-multiforme-cell-lines
#15
Yung-Lung Chang, Li-Chun Huang, Ying-Chuan Chen, Yi-Wen Wang, Dueng-Yuan Hueng, Shih-Ming Huang
Glioblastoma multiforme (GBM) is the most common primary central nervous system malignant tumor. It responds poorly to standard therapies, such as surgical resection, radiation therapy and chemotherapy. Many chemotherapeutic drugs are focused on apoptosis induction and radiation sensitivity. Inhibition of histone acetylation via histone deacetylase inhibitor (HDACI) is one such strategy. Statins (or 3-hydroxy-3-methylglutaryl-CoA reductase inhibitors) are classical drugs used to lower cholesterol but also inhibitors of histone deacetylation activity...
October 7, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28991639/haemanthamine-alters-sodium-butyrate-induced-histone-acetylation-p21-waf1-cip1-expression-chk1-and-chk2-activation-and-leads-to-increased-growth-inhibition-and-death-in-a2780-ovarian-cancer-cells
#16
Martina Seifrtová, Radim Havelek, Lucie Cahlíková, Daniela Hulcová, Naděžda Mazánková, Martina Řezáčová
BACKGROUND: Haemanthamine (HA) and sodium butyrate (NaB) are promising candidates for chemotherapy as a treatment for cancer. PURPOSE: We aimed to determine the anticancer potential of HA and NaB, alone and in combination, in A2780 ovarian cancer cells and concurrently investigated anticancer potential in contrast to non-cancer human MRC-5 fibroblasts. METHODS: Antiproliferative effects were determined by WST-1 assay and by Trypan blue exclusion staining...
November 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28991411/structural-insights-into-the-nuclear-import-of-the-histone-acetyltransferase-mof-by-importin-%C3%AE-1
#17
Weili Zheng, Rui Wang, Xi Liu, Siyu Tian, Benqiang Yao, Ang Chen, Shikai Jin, Yong Li
The histone acetyltransferase MOF (males-absent-on-the-first) acetylates the histone H4, a modification important for many biological processes, including chromatin organization, transcriptional regulation, DNA replication, recombination and repair, as well as autophagy. Depletion of MOF induces serious consequences due to the reduction of histone acetylation, such as nuclear morphological defects and cancer. Despite the critical roles of MOF in the nucleus, the structural or functional mechanisms of the nucleocytoplasmic transport of MOF remain elusive...
October 9, 2017: Traffic
https://www.readbyqxmd.com/read/28984569/nonomuraea-cavernae-sp-nov-a-novel-actinobacterium-isolated-from-a-karst-cave-sample
#18
Bao-Zhu Fang, Zheng-Shuang Hua, Ming-Xian Han, Zi-Tong Zhang, Yi-Huan Wang, Zi-Wen Yang, Wan-Qin Zhang, Min Xiao, Wen-Jun Li
A novel actinobacterial strain, designated SYSU K10005(T), was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic and Gram-stain-positive. On the basis of 16S rRNA gene sequence analysis, strain SYSU K10005(T) was most closely related to the type strains of the genus Nonomuraea, and shared highest sequence similarity of 98.4 % with Nonomuraea candida HMC10(T)...
October 6, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28981125/total-chemical-synthesis-of-histones-and-their-analogs-assisted-by-native-chemical-ligation-and-palladium-complexes
#19
Suman Kumar Maity, Muhammad Jbara, Guy Mann, Guy Kamnesky, Ashraf Brik
Chemical synthesis of histones allows precise control of the installation of post-translational modifications via the coupling of derivatized amino acids. Shortcomings of other approaches for obtaining modified histones for epigenetic studies include heterogeneity of the obtained product and difficulties in incorporating multiple modifications on the same histone. In this protocol, unprotected peptide fragments are prepared by Fmoc solid-phase synthesis and coupled in aqueous buffers via native chemical ligation (NCL; in NCL, a peptide bond is formed between a peptide with an N-terminal Cys and another peptide having a C-terminal thioester)...
November 2017: Nature Protocols
https://www.readbyqxmd.com/read/28978489/development-and-validation-of-a-sensitive-assay-for-analysis-of-midazolam-free-and-conjugated-1-hydroxymidazolam-and-4-hydroxymidazolam-in-pediatric-plasma-application-to-pediatric-pharmacokinetic-study
#20
Ganesh S Moorthy, Harini Jogiraju, Christina Vedar, Athena F Zuppa
Pharmacokinetic, pharmacodynamic and pharmacogenomic studies of midazolam are currently being performed in critically ill children to find suitable dose regimens. Sensitive assays using small volumes of plasma are necessary to determine the concentrations of midazolam and its respective metabolites in pediatric studies. Midazolam is metabolized to hydroxylated midazolam isomers, which are present as free as well as the corresponding glucuronide conjugates. A high-performance liquid chromatographic method with tandem mass spectrometry has been developed and validated for the quantification of midazolam, and free and total 1-hydroxymidazolam and 4-hydroxymidazolam metabolites in small volumes of plasma...
September 28, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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