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Sanjay Singh, Sarfraj Ahmad Siddiqui, Sukanya Tripathy, Shiv Kumar, Sudipta Saha, Rajesh Ugale, Dinesh Raj Modi, Anand Prakash
In the last few decades, there has been exponential increase in studies aiming to trace the molecular mechanism of fear extinction with a hope to minimize the return of fear after exposure therapy required for operational treatment of anxiety disorders. The present study explored how the timing of extinction training after developing a specific fear, affects the consequent return of the extinguished fear and the role of histone acetylation in controlling the circuitry, thereof. It was found that rats undergone extinction training 10 min...
June 14, 2018: Brain Research Bulletin
Yingjie Li, Wenfeng Li, Qingnan Liu, Hong Meng, Yingzhou Lu, Chunxi Li
For the first time, a series of alkynyl carbon materials (ACMs) were prepared via the mechanochemical reaction of CaC2 with six polyhalogenated precursors, namely CCl4 , C2 Cl6 , C2 Cl4 , C6 Cl6 , C6 Br6 , and C14 H4 Br10 (ACM-1, ACM-2, ACM-3, ACM-4, ACM-5, and ACM-6, respectively) and used for the adsorptive removal of mercury from aqueous solutions. Based on preliminary investigations, the adsorption of mercury on ACM-5 was studied in depth. Specifically, the effect of pH on mercury adsorptivity, adsorption kinetics, thermodynamics, isotherms, and recyclability was studied...
June 2018: Journal of Environmental Sciences (China)
Emmanuelle Gouot, Wajid Bhat, Anne Rufiange, Eric Fournier, Eric Paquet, Amine Nourani
CK2 is an essential protein kinase implicated in various cellular processes. In this study, we address a potential role of this kinase in chromatin modulations associated with transcription. We found that CK2 depletion from yeast cells leads to replication-independent increase of histone H3K56 acetylation and global activation of H3 turnover in coding regions. This suggests a positive role of CK2 in maintenance/recycling of the histone H3/H4 tetramers during transcription. Interestingly, strand-specific RNA-seq analyses show that CK2 inhibits global cryptic promoters driving both sense and antisense transcription...
June 14, 2018: Nucleic Acids Research
Shaofei Ji, Iwen Fu, Spandana Naldiga, Hongzhao Shao, Ashis K Basu, Suse Broyde, Natalia Y Tretyakova
5-Formylcytosine (5fC) is an epigenetic DNA modification introduced via TET protein-mediated oxidation of 5-methyl-dC. We recently reported that 5fC form reversible DNA-protein conjugates (DPCs) with histone proteins in living cells (Ji et al. (2017) Angew. Chem. Int. Ed., 56:14130-14134). We now examined the effects of 5fC mediated DPCs on DNA replication. Synthetic DNA duplexes containing site-specific DPCs between 5fC and lysine-containing proteins and peptides were subjected to primer extension experiments in the presence of human translesion synthesis DNA polymerases η and κ...
June 14, 2018: Nucleic Acids Research
Ivan Corbeski, Klemen Dolinar, Hans Wienk, Rolf Boelens, Hugo van Ingen
Genome replication, transcription and repair require the assembly/disassembly of the nucleosome. Histone chaperones are regulators of this process by preventing formation of non-nucleosomal histone-DNA complexes. Aprataxin and polynucleotide kinase like factor (APLF) is a non-homologous end-joining (NHEJ) DNA repair factor that possesses histone chaperone activity in its acidic domain (APLFAD). Here, we studied the molecular basis of this activity using biochemical and structural methods. We find that APLFAD is intrinsically disordered and binds histone complexes (H3-H4)2 and H2A-H2B specifically and with high affinity...
June 14, 2018: Nucleic Acids Research
Xiangyan Shi, Chinmayi Prasanna, Toshio Nagashima, Toshio Yamazaki, Konstantin Pervushin, Lars Nordenskiöld
Eukaryotic chromatin structure and dynamics play key roles in genomic regulation. In the current study, the secondary structure and intramolecular dynamics of human histone H4 (hH4) in the nucleosome core particle (NCP) and in a nucleosome array are determined by solid-state NMR (SSNMR). Secondary structure elements are successfully localized in the hH4 in the NCP precipitated with Mg2+. In particular, dynamics on nanosecond to microsecond and microsecond to millisecond timescales are elucidated, revealing diverse internal motions in the hH4 protein...
June 14, 2018: Angewandte Chemie
Mauro Fianchini, Nuno A G Bandeira
Ethylene efficiently binds late transition metals of groups 10 and 11. In spite of their reactivity, homoleptic compounds of formula [M-(η2 -C2 H4 )3 ]n+ (with n = 0,1) have been isolated in solution and solid state and characterized spectroscopically throughout the last 50 years with metals from groups 10 and 11. X-ray diffraction studies proved that such homoleptic adducts adopt planar "wheel" structures where ethylene moieties lies flat in the same plane both in group 10 and 11. These experimental findings were also confirmed by several in-depth computational investigations carried out to understand the bond pattern of such peculiar structures...
June 14, 2018: Journal of Molecular Modeling
Chizoba I Ezugwu, Md Ali Asraf, Xiao Li, Shengwei Liu, Chih-Ming Kao, Serge Zhuiykov, Francis Verpoort
Industrial dye effluents with low biodegradability are highly toxic and carcinogenic on both human and aquatic lives, thus they are detrimental to the biodiversity of environment. Herein, this data set presents the potential of cationic Nickel based MOFs in the adsorption of charged and neutral dye molecules. Data set include a concise description of experimental conditions for the synthesis of imidazolium ligands, 1,3-bis(4-carboxyphenyl)imidazolium chloride (H2 L+ Cl- ) and 1,3-bis(3,5-dicarboxyphenyl)imidazolium chloride (H4 L+ Cl- ), and MOFs...
June 2018: Data in Brief
Qing Zhao, Zhi-Li Wei, Quan-Peng Kang, Han Zhang, Wen-Kui Dong
Four homo/heterometallic complexes [Cu3 (L)(μ2 -OAc)9 (CH3 OH) 9 ]·3CHCl3 (1), [Cu2 (L)Ca(μ2 -NO3 )9 ] (9), [{Cu2 (L)Sr(μ2 -NO3 )9 }9 ]·CH3 CH2 OH (11) and [Cu2 (L)Ba(μ2 -OAc)9 (OAc)] (14), containing an acyclic naphthalenediol-based ligand H4 L, were synthesized and characterized by elemental analyses, IR, UV-Vis, fluorescence spectra, TG-DTA and X-ray crystallography. The complex 1 was obtained by the reaction of H4 L with 11 equivalents of Cu(OAc) 9 ·2H2 O. The heterometallic complexes 9, 11, 14 were acquired by the reaction of H4 L with 9 equivalents of Cu(OAc)9 ·2H2 O or Cu(NO3 )9 ·2H2 O and 1 equivalent of M(OAc)9 (M = Ca, Sr and Ba)...
June 2, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Khac Hong Nguyen, Yuanqiang Hao, Wansong Chen, Yintang Zhang, Maotian Xu, Minghui Yang, You-Nian Liu
Hydrazine (N2 H4 ) is an important and commonly used chemical reagent for the preparation of textile dyes, pharmaceuticals, pesticides and so on. Despite its widespread industrial applications, hydrazine is highly toxic and exposure to this chemical can cause many symptoms and severe damage to the liver, kidneys, and central nervous system. As a consequence, many efforts have been devoted to the development of fluorescent probes for the selective sensing and/or imaging of N2 H4 . Although great efforts have been devoted in this area, the large number of important recent studies have not yet been systematically discussed in a review format so far...
June 13, 2018: Luminescence: the Journal of Biological and Chemical Luminescence
Joseph R Podojil, Ming-Yi Chiang, Igal Ifergan, Ronald Copeland, Linda N Liu, Sebastien Maloveste, Solomon Langermann, David Liebenson, Roumen Balabanov, Hongbo Chi, Lieping Chen, Dario A A Vignali, Stephen D Miller
The potent immune regulatory function of an agonistic B7-H4-Ig fusion protein (B7-H4Ig) has been demonstrated in multiple experimental autoimmune models; however, the identity of a functional B7-H4 receptor remained unknown. The biological activity of B7-H4 is associated with decreased inflammatory CD4+ T cell responses as supported by a correlation between B7-H4-expressing tumor-associated macrophages and Foxp3+ T cells within the tumor microenvironment. Recent data indicate that members of the semaphorin (Sema)/plexin/neuropilin (Nrp) family of proteins both positively and negatively modulate immune cell function...
June 13, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
Susanne Bury, Manonmani Soundararajan, Richa Bharti, Rudolf von Bünau, Konrad U Förstner, Tobias A Oelschlaeger
Shiga toxin (Stx) producing E. coli (STEC) such as Enterohemorrhagic E. coli (EHEC) are the major cause of foodborne illness in humans. In vitro studies showed the probiotic Escherichia coli strain Nissle 1917 (EcN) to efficiently inhibit the production of Stx. Life threatening EHEC strains as for example the serotype O104:H4, responsible for the great outbreak in 2011 in Germany, evolutionary developed from certain E. coli strains which got infected by stx2 -encoding lambdoid phages turning the E. coli into lysogenic and subsequently Stx producing strains...
2018: Frontiers in Microbiology
Beatrice Berti, Cristiana Cesari, Francesco Conte, Iacopo Ciabatti, Cristina Femoni, Maria Carmela Iapalucci, Federico Vacca, Stefano Zacchini
The reactions of [Pt6 (CO)12 ]2- with CH(PPh2 )2 (dppm), CH2 ═C(PPh2 )2 (P^P), and Fe(C5 H4 PPh2 )2 (dppf) proceed via nonredox substitution and result in the heteroleptic Chini-type clusters [Pt6 (CO)10 (dppm)]2- , [Pt6 (CO)10 (P^P)]2- , and [Pt6 (CO)10 (dppf)]2- , respectively. Conversely, the reactions of [Pt6 (CO)12 ]2- with Ph2 P(CH2 )4 PPh2 (dppb) and Ph2 PC≡CPPh2 (dppa) can be described as redox fragmentation that afford the neutral complexes Pt(dppb)2 , Pt2 (CO)2 (dppa)3 , and Pt8 (CO)6 (PPh2 )2 (C≡CPPh2 )2 (dppa)2 ...
June 11, 2018: Inorganic Chemistry
Julia Costa, Maren Gatermann, Manfred Nimtz, Sebastian Kandzia, Markus Glatzel, Harald S Conradt
Cells release vesicles to the surroundings, the extracellular vesicles (EVs), which may transmit biomolecules to other cells, and are found in bodily fluids, thus constituting emerging biomarker targets. Many studies on EV nucleic acid, lipid and protein composition are available, however, detailed characterization of protein glycosylation has been less approached. Here, we describe a strategy for high resolution quantitative profiling and structure elucidation of N-glycans from EV glycoproteins of three cell lines: human HEK-293, human glioma H4 and mouse glioma Tu-2449...
June 11, 2018: Analytical Chemistry
Fei-Hong Liang, Xun-Rui Hou, Li-Hong Li, Xin Liang, Yu-Wei Lu, Hong Yang, Yuan Zhang
OBJECTIVE: To observe the effect of acupoint injection at "Yingxiang"(LI 20) and "Yintang"(GV 29) on nasal allergic reactions and the expression of histamine receptor H 1 and H 4 in nasal mucosa of allergic rhinitis (AR) rats, so as to reveal its mechanism underlying improvement of AR. METHODS: SD rats were randomized into normal, model, non-acupoint injection and acupoint injection groups ( n =8 in each). The AR model was established by intraperitoneal (i...
April 25, 2018: Zhen Ci Yan Jiu, Acupuncture Research
Irene Righetto, Francesco Filippini
Host jump can result in deadly pandemic events when avian influenza A viruses broaden their host specificity and become able to infect mammals, including humans. Haemagglutinin-the major capsid protein in influenza A viruses-is subjected to high rate mutations, of which several occur at its "head": the receptor-binding domain that mediates specific binding to host cell receptors. Such surface-changing mutations may lead to antigenically novel influenza A viruses hence in pandemics by host jump and in vaccine escape by antigenic drift...
2018: BioMed Research International
Joseph Guevara-Luna, Patricia Alvarez-Fitz, Elvira Ríos-Leal, Macdiel Acevedo-Quiroz, Sergio Encarnación-Guevara, Ma Elena Moreno-Godinez, Mildred Castellanos-Escamilla, Jeiry Toribio-Jiménez, Yanet Romero-Ramírez
Benzo[a]pyrene (BaP) is recognized as a potentially carcinogenic and mutagenic hydrocarbon, and thus, its removal from the environment is a priority. The use of thermophilic bacteria capable of biodegrading or biotransforming this compound to less toxic forms has been explored in recent decades, since it provides advantages compared to mesophilic organisms. This study assessed the biotransformation of BaP by the thermophilic bacterium Bacillus licheniformis M2-7. Our analysis of the biotransformation process mediated by strain M2-7 on BaP shows that it begins during the first 3 h of culture...
June 9, 2018: World Journal of Microbiology & Biotechnology
C Charles-Schoeman, G B Gugiu, H Ge, A Shahbazian, Y Y Lee, X Wang, D E Furst, V K Ranganath, M Maldonado, Terry Lee, S T Reddy
BACKGROUND AND AIMS: To evaluate changes in the high-density lipoprotein (HDL) proteome and HDL function in active rheumatoid arthritis (RA) patients initiating therapy with abatacept or adalimumab in the Abatacept Versus Adalimumab Comparison in Biologic-Naïve RA Subjects with Background Methotrexate (AMPLE) study. METHODS: Ultra high-pressure liquid chromatography (UHPLC) coupled with ion mobility mass spectrometry (LC-IM-MS) was used to analyze proteins associated with immunoaffinity-captured HDL from plasma of 30 patients with RA randomized to either abatacept (n = 15) or adalimumab (n = 15) therapy...
April 17, 2018: Atherosclerosis
Axel D Becke, Stephen G Dale, Erin R Johnson
It has been known for over twenty years that density functionals of the generalized-gradient approximation (GGA) type and exact-exchange-GGA hybrids with low exact-exchange mixing fraction yield enormous errors in the properties of charge-transfer (CT) complexes. Manifestations of this error have also plagued computations of CT excitation energies. GGAs transfer far too much charge in CT complexes. This error has therefore come to be called "delocalization" error. It remains, to this day, a vexing unsolved problem in density-functional theory (DFT)...
June 7, 2018: Journal of Chemical Physics
Tao Wang, Matthew V Holt, Nicolas L Young
BACKGROUND: The dynamics of histone post-translational modifications (PTMs) are sparsely described, especially in their true physiological context of proteoforms (single histone molecules harboring combinations of PTMs). METHODS: Here we time-resolve the response of cells to SUV4-20 methyltransferase inhibition and unbiasedly quantitate the dynamic response of histone H4 PTMs and proteoforms. RESULTS: Contrary to the prevailing dogma, cells exhibit an immediate-early response with changes to histone proteoforms...
June 7, 2018: Epigenetics & Chromatin
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