keyword
MENU ▼
Read by QxMD icon Read
search

H4

keyword
https://www.readbyqxmd.com/read/29209217/arylacetamide-deacetylase-is-involved-in-vicagrel-bioactivation-in-humans
#1
Jinfang Jiang, Xiaoyan Chen, Dafang Zhong
Vicagrel, a structural analog of clopidogrel, is now being developed as a thienopyridine antiplatelet agent in a phase II clinical trial in China. Some studies have shown that vicagrel undergoes complete first-pass metabolism in human intestine, generating the hydrolytic metabolite 2-oxo-clopidogrel via carboxylesterase-2 (CES2) and subsequently the active metabolite H4 via CYP450s. This study aimed to identify hydrolases other than CES2 that are involved in the bioactivation of vicagrel in human intestine...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29198113/characterization-of-chlamydomonas-reinhardtii-core-histones-by-top-down-mass-spectrometry-reveals-unique-algae-specific-variants-and-post-translational-modifications
#2
Aliyya Khan, Carlo K Eikani, Hana Khan, Anthony T Iavarone, James J Pesavento
The unicellular microalga Chlamydomonas reinhardtii has played an instrumental role in the development of many new fields (bio-products, biofuels, etc.) as well as the advancement of basic science (photosynthetic apparati, flagellar function, etc.). Chlamydomonas' versatility ultimately derives from the genes encoded in its genome and the way that the expression of these genes is regulated, which is largely influenced by a family of DNA binding proteins called histones. In this study, we characterize C. reinhardtii core histones, both variants and their post-translational modifications, by chromatographic separation followed by top-down mass spectrometry (TDMS)...
December 3, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29192002/ms-western-a-method-of-multiplexed-absolute-protein-quantification-is-a-practical-alternative-to-western-blotting
#3
Mukesh Kumar, Shai R Joseph, Martina Augsburg, Aliona Bogdanova, David Drechsel, Nadine L Vastenhouw, Frank Buchholz, Marc Gentzel, Andrej Shevchenko
Absolute quantification of proteins elucidates the molecular composition, regulation and dynamics of multiprotein assemblies and networks. Here we report on a method termed MS Western that accurately determines the molar abundance of dozens of user-selected proteins at the sub-femtomole level in whole cell or tissue lysates without metabolic or chemical labelling and without using specific antibodies. MS Western relies upon GeLC-MS/MS and quantifies proteins by in-gel co-digestion with an isotopically labelled QconCAT protein chimera composed of concatenated proteotypic peptides...
November 30, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29190910/the-tissue-distribution-and-significance-of-b7-h4-in-laryngeal-carcinoma
#4
Lili Chen, Meihua Jin, Chunshi Li, Yongjun Shang, Qinggao Zhang
The costimulatory signals CD28 and B7 have been shown to control tumor invasion and metastasis by regulating T cell activation, whereas the distribution characteristics of B7-associated proteins in laryngeal carcinoma (LC) tissue are still unclear. Here, the expression of members of the B7 superfamily, including B7-H1 (PD-L1), B7-DC (PD-L2) and B7-H4, in fifty-two LC samples was determined by immunohistochemistry, and the relationship between B7-H4 and epithelial-mesenchymal transition (EMT)-associated markers was further assessed by immunofluorescence double staining...
November 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/29189263/b7-h4-is-inversely-correlated-with-t-cell-infiltration-in-clear-cell-but-not-serous-or-endometrioid-ovarian-cancer
#5
Gabriel M Pagnotti, Richard M Atkinson, Jamie Romeiser, Ali Akalin, Mallory B Korman, Kenneth R Shroyer
B7-H4, a tumor-associated cell surface protein, is expressed in endometrioid (EM), serous (SE), and clear cell (CC) ovarian carcinomas. Prior in vitro studies from other groups indicated that elevated B7-H4 expression by tumor cells blocks T-cell activation; therefore, it had been postulated to play a role in shielding cancer cells from immune surveillance and averting apoptotic programs. To test the validity of these hypotheses, the present study was designed to compare the immunohistochemical staining intensity of B7-H4 in tumor cells of ovarian cancers with the number of tumor-infiltrating T cells and macrophages and with the levels of caspase-3 staining in apoptotic debris...
October 20, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/29187567/linking-phytochrome-interacting-factor-to-histone-modification-in-plant-shade-avoidance
#6
Maolin Peng, Zepeng Li, Nana Zhou, Mengmeng Ma, Yupei Jiang, Aiwu Dong, Wen-Hui Shen, Lin Li
Shade avoidance syndrome (SAS) allows a plant grown in densely populated environment to maximize opportunities to access to sunlight. While it is well established that SAS is accompanied by gene expression changes, the underlying molecular mechanism needs to be elucidated. Here, we identify the H3K4me3/H3K36me3-binding proteins, Morf Related Gene (MRG) group proteins MRG1 and MRG2, as positive regulators of shade-induced hypocotyl elongation in Arabidopsis thaliana. MRG2 binds PHYTOCHROME- INTERACTING FACTOR 7 (PIF7) and regulates the expression of several common downstream target genes, including YUCCA8 and IAA19 involved in the auxin biosynthesis or response pathway and PRE1 involved in brassinosteroid regulation of cell elongation...
November 29, 2017: Plant Physiology
https://www.readbyqxmd.com/read/29186677/the-methyltransferase-setd8-is-essential-for-erythroblast-survival-and-maturation
#7
Jeffrey Malik, Jacquelyn A Lillis, Tyler Couch, Michael Getman, Laurie A Steiner
Erythropoiesis is a highly regulated process that generates enucleate red blood cells from committed erythroid progenitors. Chromatin condensation culminating in enucleation is a defining feature of this process. Setd8 is the sole enzyme that can mono-methylate histone H4, lysine 20 and is highly expressed in erythroblasts compared to most other cell types. Erythroid Setd8 deletion results in embryonic lethality from severe anemia due to impaired erythroblast survival and proliferation. Setd8 protein levels are also uniquely regulated in erythroblasts, suggesting a cell-type-specific role for Setd8 during terminal maturation...
November 28, 2017: Cell Reports
https://www.readbyqxmd.com/read/29186293/early-histone-h4-acetylation-during-chromatin-remodeling-in-equine-spermatogenesis
#8
Chelsea C Ketchum, Casey D Larsen, Alexis McNeil, Mirella L Meyer-Ficca, Ralph G Meyer
Chromatin remodeling during spermatogenesis culminates in the exchange of nucleosomes for transition proteins and protamines as an important part of spermatid development to give rise to healthy sperm. Comparative immunofluorescence analyses of equine and murine testis histological sections were used to characterize nucleoprotein exchange in the stallion. Histone H4 hyperacetylation is considered a key event of histone removal during the nucleoprotein transition to a protamine-based sperm chromatin structure...
November 25, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29184503/pharmacological-characterization-of-5-substituted-1-2-3-dihydro-1-benzofuran-2-yl-methyl-piperazines-novel-antagonists-for-the-histamine-h3-and-h4-receptors-with-anti-inflammatory-potential
#9
Michelle F Corrêa, Álefe J R Barbosa, Larissa B Teixeira, Diego A Duarte, Sarah C Simões, Lucas T Parreiras-E-Silva, Aleksandro M Balbino, Richardt G Landgraf, Michel Bouvier, Claudio M Costa-Neto, João P S Fernandes
The histamine receptors (HRs) are traditional G protein-coupled receptors of extensive therapeutic interest. Recently, H3R and H4R subtypes have been targeted in drug discovery projects for inflammation, asthma, pain, cancer, Parkinson's, and Alzheimer's diseases, which includes searches for dual acting H3R/H4R ligands. In the present work, nine 1-[(2,3-dihydro-1-benzofuran-2-yl)methyl]piperazine (LINS01 series) molecules were synthesized and evaluated as H3R and H4R ligands. Our data show that the N-allyl-substituted compound LINS01004 bears the highest affinity for H3R (pKi 6...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29184082/identification-of-a-histone-family-gene-signature-for-predicting-the-prognosis-of-cervical-cancer-patients
#10
Xiaofang Li, Run Tian, Hugh Gao, Yongkang Yang, Bryan R G Williams, Michael P Gantier, Nigel A J McMillan, Dakang Xu, Yiqun Hu, Yan'e Gao
Heterogeneity in terms of tumor characteristics, prognosis, and survival among cancer patients is an unsolved issue. Here, we systematically analyzed the aberrant expression patterns of cervical cancer using RNA-Seq data from The Cancer Genome Atlas (TCGA). We incorporated gene profiling, molecular signatures, functional and pathway information with gene set enrichment and protein-protein interaction (PPI) network analysis, to identify sub-networks of genes. Those identified genes relating to DNA replication and DNA repair-mediated signaling pathways associated with systemic lupus erythematosus (SLE)...
November 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29182104/steered-molecular-dynamics-for-studying-ligand-unbinding-of-ecdysone-receptor
#11
Xueping Hu, Song Hu, Jiazhe Wang, Yawen Dong, Li Zhang, Yanhong Dong
Ecdysone receptor (EcR) is an important target for pesticide design. Ligand binding regulates EcR transcriptional activity similar to other nuclear receptors; however, the pathways by which ligands enter and leave the EcR remain poorly understood. Here, we performed computational studies to identify unbinding pathways of an ecdysone agonist [the selective ecdysone agonist, BYI06830] from the EcR ligand binding domain (EcR LBD). BYI06830 can dissociate from EcR LBD via four different pathways with little effect on receptor structure...
November 28, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29180474/vitamin-c-sensitizes-melanoma-to-bet-inhibitors
#12
Gaofeng Wang, Sushmita Mustafi, Vladimir Camarena, Claude-Henry Volmar, Tyler C Huff, David W Sant, Shaun P Brothers, Zhao-Jun Liu, Claes Wahlestedt
Bromodomain and extra-terminal inhibitors (BETi) are promising cancer therapies, yet prominent side effects of BETi at effective doses have been reported in Phase I clinical trials. Here we screened a panel of small molecules targeting epigenetic modulators against human metastatic melanoma cells. Cells were pretreated with or without ascorbate (vitamin C), which promotes DNA demethylation and subsequently changes the sensitivity to drugs. Top hits were structurally unrelated BETi including JQ1, I-BET151, CPI-203, and BI-2536...
November 27, 2017: Cancer Research
https://www.readbyqxmd.com/read/29179736/phylogenetic-analysis-of-the-core-histone-doublet-and-dna-topo-ii-genes-of-marseilleviridae-evidence-of-proto-eukaryotic-provenance
#13
Albert J Erives
BACKGROUND: While the genomes of eukaryotes and Archaea both encode the histone-fold domain, only eukaryotes encode the core histone paralogs H2A, H2B, H3, and H4. With DNA, these core histones assemble into the nucleosomal octamer underlying eukaryotic chromatin. Importantly, core histones for H2A and H3 are maintained as neofunctionalized paralogs adapted for general bulk chromatin (canonical H2 and H3) or specialized chromatin (H2A.Z enriched at gene promoters and cenH3s enriched at centromeres)...
November 28, 2017: Epigenetics & Chromatin
https://www.readbyqxmd.com/read/29179726/modulation-of-the-functional-association-between-the-hiv-1-intasome-and-the-nucleosome-by-histone-amino-terminal-tails
#14
Mohamed S Benleulmi, Julien Matysiak, Xavier Robert, Csaba Miskey, Eric Mauro, Delphine Lapaillerie, Paul Lesbats, Stéphane Chaignepain, Daniel R Henriquez, Christina Calmels, Oyindamola Oladosu, Eloïse Thierry, Oscar Leon, Marc Lavigne, Marie-Line Andreola, Olivier Delelis, Zoltán Ivics, Marc Ruff, Patrice Gouet, Vincent Parissi
BACKGROUND: Stable insertion of the retroviral DNA genome into host chromatin requires the functional association between the intasome (integrase·viral DNA complex) and the nucleosome. The data from the literature suggest that direct protein-protein contacts between integrase and histones may be involved in anchoring the intasome to the nucleosome. Since histone tails are candidates for interactions with the incoming intasomes we have investigated whether they could participate in modulating the nucleosomal integration process...
November 28, 2017: Retrovirology
https://www.readbyqxmd.com/read/29175728/on-the-contributions-of-photorespiration-and-compartmentation-to-the-contrasting-intramolecular-2h-profiles-of-c3-and-c4-plant-sugars
#15
Youping Zhou, Benli Zhang, Hilary Stuart-Williams, Kliti Grice, Charles H Hocart, Arthur Gessler, Zachary E Kayler, Graham D Farquhar
Compartmentation of C4 photosynthetic biochemistry into bundle sheath (BS) and mesophyll (M) cells, and photorespiration in C3 plants is predicted to have hydrogen isotopic consequences for metabolites at both molecular and site-specific levels. Molecular-level evidence was recently reported (Zhou et al., 2016), but evidence at the site-specific level is still lacking. We propose that such evidence exists in the contrasting 2H distribution profiles of glucose samples from naturally grown C3, C4 and CAM plants: photorespiration contributes to the relative 2H enrichment in H5 and relative 2H depletion in H1 & H6 (the average of the two pro-chiral Hs and in particular H6,pro-R) in C3 glucose, while 2H-enriched C3 mesophyll cellular (chloroplastic) water most likely contributes to the enrichment at H4; export of (transferable hydrogen atoms of) NADPH from C4 mesophyll cells to bundle sheath cells (via the malate shuttle) and incorporation of 2H-relatively unenriched BS cellular water contribute to the relative depletion of H4 & H5 respectively; shuttling of triose-phosphates (PGA: phosphoglycerate dand DHAP: dihydroacetone phosphate) between C4 bundle sheath and mesophyll cells contributes to the relative enrichment in H1 & H6 (in particular H6,pro-R) in C4 glucose...
November 23, 2017: Phytochemistry
https://www.readbyqxmd.com/read/29166391/early-kinetics-of-serum-interleukine-17a-and-infarct-size-in-patients-with-reperfused-acute-st-elevated-myocardial-infarction
#16
Thomas Bochaton, Nathan Mewton, NDieme Thiam, Fabien Lavocat, Delphine Baetz, Nathalie Dufay, Cyril Prieur, Eric Bonnefoy-Cudraz, Pierre Miossec, Michel Ovize
BACKGROUND: Recently, it was shown that interleukin-17A (IL-17A) is involved in the pathophysiology of reperfusion injury and associated with infarct size (IS) in experimental models of myocardial infarction. Our aim was to evaluate whether the IL-17A serum level and the IL-17A active fraction was correlated with IS in humans. METHODS: 101 patients presenting with a ST-elevated Myocardial Infarction (STEMI) referred for primary percutaneous coronary intervention (PPCI) and 10 healthy controls were included...
2017: PloS One
https://www.readbyqxmd.com/read/29163825/maternal-histone-acetyltransferase-kat8-is-required-for-porcine-preimplantation-embryo-development
#17
Zubing Cao, Ronghua Wu, Di Gao, Tengteng Xu, Lei Luo, Yunsheng Li, Jianyong Han, Yunhai Zhang
K (lysine) acetyltransferase 8 (KAT8), an acetyltransferase that specifically catalyzes histone H4 lysine 16 acetylation, is critical for key biological processes including cell proliferation and maintenance of genome stability. However, the role of KAT8 during preimplantation development in pigs remains unclear. Results herein showed that KAT8 mRNA is maternally derived and it is required for successful development of early embryos. An abundance of KAT8 transcripts are expressed in oocytes and its abundance continuously decreases throughout meiotic maturation and preimplantation development...
October 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/29161255/neurogenic-mediators-contribute-to-local-edema-induced-by-micrurus-lemniscatus-venom
#18
Luciana Lyra Casais-E-Silva, Catarina Teixeira
BACKGROUND/AIMS: Micrurus is one of the four snake genera of medical importance in Brazil. Coral snakes have a broad geographic distribution from the southern United States to Argentina. Micrurine envenomation is characterized by neurotoxic symptoms leading to dyspnea and death. Moreover, various local manifestations, including edema formation, have been described in patients bitten by different species of Micrurus. Thus, we investigated the ability of Micrurus lemniscatus venom (MLV) to induce local edema...
November 21, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29160198/herbidospora-soli-sp-nov-isolated-from-soil
#19
Nantawan Niemhom, Chitti Thawai
A novel actinomycete strain PS42-9(T), which formed short chains of spores borne on the tip of long sporophores arising from the substrate mycelium, was isolated from soil in Phu-Sang National Park, Phayao province, Thailand. The isolate contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars of strain PS42-9(T) were glucose, madurose, mannose, rhamnose and ribose. The characteristic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive glycophospholipids...
November 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29159585/kfoa-the-udp-glucose-4-epimerase-of-escherichia-coli-strain-o5-k4-h4-shows-preference-for-acetylated-substrates
#20
Hao-Miao Zhu, Bin Sun, Yi-Jun Li, Dan-Hua Meng, Shuang Zheng, Ting-Ting Wang, Feng-Shan Wang, Ju-Zheng Sheng
Capsule of Escherichia coli O5:K4:H4 is formed of a chondroitin-repeat disaccharide unit of glucuronic acid (GlcA)-N-acetylgalactosamine (GalNAc). This polysaccharide, commonly referred to as K4CP, is a potentially important source of precursors for chemoenzymatic or bioengineering synthesis of chondroitin sulfate. KfoA, encoded by a gene from region 2 of the K4 capsular gene cluster, shows high homology to the UDP-glucose-4-epimerase (GalE) from E. coli. KfoA is reputed to be responsible for uridine 5'-diphosphate-N-acetylgalactosamine (UDP-GalNAc) supply for K4CP biosynthesis in vivo, but it has not been biochemically characterized...
November 20, 2017: Applied Microbiology and Biotechnology
keyword
keyword
36216
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"