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https://www.readbyqxmd.com/read/27909867/developmental-validation-of-the-homygene19-14y-system
#1
Weian Du, Ling Chen, Hong Liu, Pingming Qiu, Fayuan Li, Jing Gao, Yu Zhou, Bangchao Wang, Chao Liu
The HomyGene19+14Y System (HG19+14Y) is a PCR-based amplification kit that enables typing of 18 autosomal short tandem repeat (STR) loci (i.e., CSF1PO, D2S1338, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, FGA, Penta E, TPOX, TH01, vWA), 14 widely used Y chromosome STR (Y-STR) loci (Y_GATA_H4, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS438, DYS439, DYS456, DYS458, DYS635), and amelogenin. This multiplex system was designed for the simultaneous analysis of amelogenin-Y allele mutation, single-source searches, kinship (including familial searching), mixture profiles, international data sharing, and other forensic applications...
December 1, 2016: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/27905050/transcriptional-analysis-of-genes-encoding-%C3%AE-glucosidase-of-schizophyllum-commune-kuc9397-under-optimal-conditions
#2
Young Min Lee, Hanbyul Lee, Young Mok Heo, Hwanhwi Lee, Joo-Hyun Hong, Jae-Jin Kim
The present study was conducted to determine the gene responsible for beta-glucosidase (BGL) production and to generate a full-length complementary DNA (cDNA) of one of the putative BGL genes, which showed a significant expression level when Schizophyllum commune KUC9397 was grown in optimized medium. The relative expression levels of seven genes encoding BGL of S. commune KUC9397 were determined with real-time quantitative reverse transcription PCR in cellulose-containing optimized medium (OM) compared to glucose-containing basal medium (BM)...
November 30, 2016: Folia Microbiologica
https://www.readbyqxmd.com/read/27904655/increased-set1-binding-at-the-promoter-induces-aberrant-epigenetic-alterations-and-up-regulates-cyclic-adenosine-5-monophosphate-response-element-modulator-alpha-in-systemic-lupus-erythematosus
#3
Qing Zhang, Shu Ding, Huilin Zhang, Hai Long, Haijing Wu, Ming Zhao, Vera Chan, Chak-Sing Lau, Qianjin Lu
BACKGROUND: Up-regulated cyclic adenosine 5'-monophosphate response element modulator α (CREMα) which can inhibit IL-2 and induce IL-17A in T cells plays a critical role in the pathogenesis of systemic lupus erythematosus (SLE). This research aimed to investigate the mechanisms regulating CREMα expression in SLE. RESULTS: From the chromatin immunoprecipitation (ChIP) microarray data, we found a sharply increased H3 lysine 4 trimethylation (H3K4me3) amount at the CREMα promoter in SLE CD4+ T cells compared to controls...
2016: Clinical Epigenetics
https://www.readbyqxmd.com/read/27902315/nocardioides-cavernae-sp-nov-a-novel-actinobacterium-isolated-from-a-karst-cave
#4
Ming-Xian Han, Bao-Zhu Fang, Ye Tian, Wan-Qin Zhang, Jian-Yu Jiao, Lan Liu, Zi-Tong Zhang, Min Xiao, Da-Qiao Wei, Wen-Jun Li
A novel actinobacterial strain, designated YIM A1136T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China. Taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic, Gram-staining-positive and rod-shaped. Colonies of strain were circular, convex, opaque and yellowish white in colour. On the basis of 16S rRNA gene sequence analysis, strain YIM A1136T is most closely related to the type strains of Nocardioides ganghwensis JC2055T (98...
November 21, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902309/flexivirga-oryzae-sp-nov-isolated-from-soil-of-a-rice-paddy-and-emended-description-of-the-genus-flexivirga-anzai-et-al-2012
#5
Jong Woo Hyeon, Hye Rim Kim, Hyo Jung Lee, Sang Eun Jeong, Sang Ho Choi, Che Ok Jeon
A Gram-staining-positive, strictly aerobic bacterium, designated R1T, was isolated from a rice paddy in South Korea. Cells were non-motile cocci showing oxidase-negative and catalase-positive activities. Growth of strain R1T was observed at 10-37 °C (optimum, 30 C) and pH 5.0-9.0 (optimum, pH 7.0). Strain R1T contained iso-C16 : 0, summed feature 9 (comprising iso-C17 : 1 ω9c/C16 : 0 10-methyl) , anteiso-C17 : 0, iso-C17 : 0 and anteiso-C17 : 1 ω9c as the major fatty acids and MK-8 (H4) and MK-8 (H6) as the isoprenoid quinones...
November 11, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902300/kibdelosporangium-metalli-sp-nov-isolated-from-rare-earth-mine-of-bayan-obo-in-china
#6
Yan-Ru Cao, Wen-Qing Yang, Ya Dao, Bin Hu, Zhong-Kan He, Lian-Ming Liang
A Gram-positive, sporangium-like globular bodies forming, oxidase-negative, catalase-positive isolate from the rare earth mine of Bayan Obo in China, designated strain KC 266T, was subjected to a comprehensive taxonomic study. Comparative 16S rRNA gene sequence analysis revealed that strain KC 266T represented a novel lineage within the genus Kibdelosporangium and showed highest 16S rRNA gene similarities to K. philippinense (98.5%), K. aridum subsp. largum (98.2%), K. aridum subsp. aridum (98.2%) and K. phytohabitans (98...
October 17, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902298/description-of-pseudactinotalea-terrae-gen-nov-sp-nov-isolated-from-greenhouse-soil-and-proposal-to-reclassify-actinotlea-suaedae-as-pseudactinotalea-suaedae-comb-nov
#7
Hayoung Cho, Moriyuki Hamada, Jae-Hyung Ahn, Hang-Yeon Weon, Jae-Ho Joa, Ken-Ichiro Suzuki, Soon-Wo Kwon, Soo-Jin Kim
One bacterial strain, designated 5GHs33-3T, was isolated from greenhouse soil collected from Yongin region, Gyeonggi province, South Korea. It was aerobic, Gram-stain-positive, flagellated, rod-shaped bacterium. The strain grew at the range of 4-37 °C (optimally 28-30 °C), pH 6.0-10.0 (optimally pH 7.0) and 0-7 % (w/v) NaCl concentrations (optimally, 0 %). The 16S rRNA gene sequence analysis revealed that it had high sequence similarity with Actinotalea suaedae EGI 60002T (98.4 %), Actinotalea ferrariae CF5-4T (96...
November 30, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902295/kribbella-deserti-sp-nov-isolated-from-rhizosphere-soil-of-ammopiptanthus-mongolicus
#8
Ji-Quan Sun, Lian Xu, Yan Guo, Wei-Le Li, Zhong-Qiu Shao, Yuan-Liang Yang, Xiao-Lei Wu
A Gram-staining-positive, aerobic bacterial strain, designated SL15-1T was isolated from desert soil which sampled from the rhizosphere of Ammopiptanthus mongolicus, Hangjin Banner, Inner Mongolia, northern China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SL15-1T was clustered with Kribbella strains, sharing the highest similarity of 16S rRNA gene (96.97%) to Kribbella sandramycini DSM 15626T. Strain SL15-1T contained diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylcholine (PC), an unknown phospholipid (PL), an unknown lipid (L) and two unknown aminophospholipids (APL) as the major polar lipids...
November 25, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902285/raineyella-antarctica-gen-nov-sp-nov-a-novel-psychrotolerant-d-amino-acid-utilizing-anaerobe-isolated-from-two-geographic-locations-of-the-southern-hemisphere
#9
Elena V Pikuta, Rodolfo Javier Menes, Alisa Michelle Bruce, Zhe Lyu, Nisha Patel, Yuchen Liu, Richard Brice Hoover, Hans Jürgen Busse, Paul A Lawson, William Barney Whitman
Gram-positive bacterium, strain LZ-22T, was isolated from a rhizosphere of moss Leptobryum sp. collected at the shore of Lake Zub in Antarctica. Cells were motile, straight or pleomorphic rods with sizes of 0.6-1.0 x 3.5-10 μm. The new isolate was a facultatively anaerobic, catalase positive, psychrotolerant mesophile. Growth was observed at 3-41 oC (optimum 24-30 oC), 0-7 % NaCl, w/v (optimum, 0.25 %) and pH 4.0-9.0 (optimum 7.8). The quinone system of strain LZ-22T possessed predominately menaquinones MK-9(H4)...
October 12, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902254/streptomyces-krungchingensis-sp-nov-isolated-from-soil-in-thailand
#10
Paranee Sripreechasak, Wongsakorn Phongsopitanun, Tomohiko Tamura, Somboon Tanasupawat
A novel actinomycete, designated KC-035T, was isolated from soil collected from Krung Ching Waterfall National Park, Nakhon Si Thammarat Province, Thailand. Its taxonomic position was determined using the polyphasic approach. The strain had morphological and chemotaxonomic properties typical of the members of the genus Streptomyces; flexuous spore chain; LL-diaminopimelic acid in cell wall peptidoglycan; MK-9(H8), MK-9(H6) and MK-9(H4) as menaquinones; diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside as phospholipids; anteiso-C15:0, C16:0, iso-C16:0, iso-C15:0 and iso-C14:0 as major cellular fatty acids; and 72 mol% of the DNA G+C content...
October 12, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902245/streptomyces-luteus-sp-nov-a-novel-actinomycete-isolated-from-loulan-soil-of-xinjiang-china
#11
Xiaoxia Luo, Kai Liang, Yang Wang, Chuanxing Wan, Lili Zhang
A Streptomyces strain, designated TRM 45540T, was isolated from the soil of Loulan area(89°22'22″E, 40°29'55″N), Xinjiang Province, north-west China and was characterized taxonomically by using a polyphasic study. Phylogenetic analysis of the strain from 16S rRNA sequence revealed the strain TRM 45540 had 99.87% similarity with the type strain Streptomyces mutabilis NRRL12800T (GenBank accession number. AB184156). The strain was aerobic, gram-staining-positive, with an optimum NaCl concentration for growth of 5% (w/v)...
November 1, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902237/krasilnikoviella-muralis-gen-nov-sp-nov-a-new-member-of-the-family-promicromonosporaceae-isolated-from-the-takamatsuzuka-tumulus-stone-chamber-interior-and-reclassification-of-promicromonospora-flava-as-krasilnikoviella-flava-comb-nov
#12
Miyuki Nishijima, Nozomi Tazato, Yutaka Handa, Nao Umekawa, Rika Kigawa, Chie Sano, Junta Sugiyama
A Gram-stain positive, facultative anaerobic actinomycete, designated strain T6220-5-2bT, was isolated from a sample taken from a mouldy spot on the surface of a mural painting (the white tiger, Byakko) inside the stone chamber of Takamatsuzuka Tumulus in Asuka village, Nara Prefecture, Japan. Based on the 16S rRNA gene sequence analysis of the isolate, it was closely related to the genus Promicromonospora, but formed of a novel lineage within the family Promicromonosporaceae. The closest related species to strain T6220-5-2bT was the known species Promicromonospora flava, which displayed a 99...
October 26, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902233/promicromonospora-kermanensis-sp-nov-a-new-actinobacterium-isolated-from-soil
#13
Fatemeh Mohammadipanah, Maria Del Carmen Montero-Calasanz, Peter Schumann, Cathrin Spröer, Hans-Peter Klenk, Manfred Rohde
A novel Promicromonospora strain, designated HM 533T, was isolated from soil in Kerman Province, Iran. It produced long and branched hyphae on ISP 2 medium that developed into a large number of irregular shaped spores. It showed optimal growth at 25-30 °C and pH 5.0-8.0 with 0-4% (w/v) NaCl. The peptidoglycan type of strain HM 533T is A4α L-Lys - L-Ala - D-Glu. Whole cell hydrolyzates of strain HM 533T contained the sugars ribose, glucose and galactose. The main phospholipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, two unknown glycolipids and an unknown phospholipid...
November 8, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902232/aeromicrobium-choanae-sp-nov-a-novel-actinobacterium-isolated-from-the-choana-of-a-garden-warbler
#14
Polona Ber, Stefanie Van Trappen, Peter Vandamme, Janja Trcek
A Gram-positive, non-spore forming actinobacterium strain 9H-4T isolated from the choana of a garden warbler (Sylvia borin) was studied to examine its taxonomic position. On the basis of the 16S rRNA gene sequence analysis it was shown that strain 9H-4T belongs to the genus Aeromicrobium with Aeromicrobium flavum (98.7 %) and Aeromicrobium tamlense (98.4 %) as the nearest neighbours and forms a separate branch in a neighbour-joining 16S rRNA gene sequence based phylogenetic tree. DNA-DNA hybridizations confirmed its novel species identity by 39 % and 46 % DNA-DNA relatedness with Aeromicrobium flavum DSM 19355T and Aeromicrobium tamlense DSM 19087T, respectively...
November 3, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902221/streptomyces-fuscichromogenes-sp-nov-a-novel-actinomycete-from-soil
#15
Hao Zhang, Jimei Zheng, Junli Zhuang, Yuhua Xin, Xiaowei Zheng, Jianli Zhang
A novel actinomycete, designated strain m16T, was isolated from a soil sample collected from Xishuangbanna's tropical rain forest, a prefecture in Yunnan Province, south-west China, and characterized by using polyphasic taxomomy. Cells were aerobic, Gram-positive and spore chains were observed to be of the helical type, with elliptical spores and smooth spore surfaces. The novel strain grew over a temperature range of 15-35 °C, at pH values of 5.0-11.0 and in the presence of 0-3 % (w/v) NaCl. The DNA G+C content of strain m16T was 70...
October 13, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27901066/greater-efficacy-of-atorvastatin-versus-a-non-statin-lipid-lowering-agent-against-renal-injury-potential-role-as-a-histone-deacetylase-inhibitor
#16
Ravi Shankar Singh, Dharmendra Kumar Chaudhary, Aradhana Mohan, Praveen Kumar, Chandra Prakash Chaturvedi, Carolyn M Ecelbarger, Madan M Godbole, Swasti Tiwari
Statins, 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase inhibitors have been shown to improve diabetic nephropathy. However, whether they provide protection via Histone deacetylases (HDAC) inhibition is not clear. We conducted a comparative evaluation of Atorvastatin (AT) versus the non-statin cholesterol-lowering drug, Ezetimibe (EZT) on severity of diabetic nephropathy. Streptozotocin-treated male Wistar rats were fed a cholesterol-supplemented diet and gavaged daily with vehicle, AT or EZT. Control rats received normal diet and gavaged vehicle (n = 8-9/group)...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27899280/behavioural-phenotype-of-histamine-h4-receptor-knockout-mice-focus-on-central-neuronal-functions
#17
Maria Domenica Sanna, Carla Ghelardini, Robin L Thurmond, Emanuela Masini, Nicoletta Galeotti
The functional expression of H4 receptors (H4R) within neurons of the central nervous system has been recently reported, but their role is poorly understood. The present study aims to elucidate the role of neuronal H4R by providing the first description of the behavioural phenotype of H4R-deficient (H4R knockout, H4R-KO) mice. Mice lacking H4R underwent behavioural studies to evaluate locomotor activity, pain perception, anxiety, depression, memory and feeding behaviour. H4R-KO mice showed a significant increase in ambulation in an open field as well as in exploratory activity in the absence of any modification of motor coordination...
November 26, 2016: Neuropharmacology
https://www.readbyqxmd.com/read/27897216/characterizations-of-h4-avian-influenza-viruses-isolated-from-ducks-in-live-poultry-markets-and-farm-in-shanghai
#18
Ying Shi, Hongrui Cui, Junheng Wang, Qiuyan Chi, Xuesong Li, Qiaoyang Teng, Hongjun Chen, Jianmei Yang, Qinfang Liu, Zejun Li
H4 avian influenza virus is one of the most prevalent influenza virus subtypes in birds. The evolution and pathogenicity of H4 AIV in domestic birds of China remain largely unclear. In the present study, a total of eight H4 AIV strains isolated in duck farm and live poultry markets (LPM) were characterized. Phylogenetic analysis indicated that these strains are divided into two groups in the Eurasian lineage. Eight genes of MH-2/H4N6 isolated from a duck farm were closely related to three H4N6 viruses from LPM, suggesting a potential AIV link between farms and LPMs...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27896686/blastococcus-colisei-sp-nov-isolated-from-an-archaeological-amphitheatre
#19
Karima Hezbri, Imen Nouioui, Manfred Rohde, Peter Schumann, Maher Gtari, Hans-Peter Klenk, Maria Del Carmen Montero-Calasanz, Faten Ghodhbane-Gtari
The taxonomic position of an actinobacterial isolate, designated strain BMG 822(T), isolated from limestone from the Amphitheater of El Jem (Coliseum Thysdrus), Tunisia, was established using a polyphasic approach. Strain BMG 822(T) was found to grow well at 30 °C and pH 6.5-8.0, and to be coral-coloured, Gram-positive, catalase and oxidase negative. Whole cell hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid, glucose, galactose and ribose. The phospholipids detected were diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, an unidentified glycophospholipid and six unidentified phospholipids...
November 28, 2016: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/27895639/netosis-as-source-of-autoantigens-in-rheumatoid-arthritis
#20
REVIEW
Elisa Corsiero, Federico Pratesi, Edoardo Prediletto, Michele Bombardieri, Paola Migliorini
In neutrophils (but also in eosinophils and in mast cells), different inflammatory stimuli induce histone deimination, chromatin decondensation, and NET formation. These web-like structures that trap and kill microbes contain DNA, cationic granule proteins, and antimicrobial peptides, but the most abundant proteins are core histones. Histones contained in NETs have been deiminated, and arginines are converted in citrullines. While deimination is a physiological process amplified in inflammatory conditions, only individuals carrying genetic predisposition to develop rheumatoid arthritis (RA) make antibodies to deiminated proteins...
2016: Frontiers in Immunology
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