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https://www.readbyqxmd.com/read/28102844/human-adipose-stem-cell-differentiation-is-highly-affected-by-cancer-cells-both-in-vitro-and-in-vivo-implication-for-autologous-fat-grafting
#1
Francesca Paino, Marcella La Noce, Diego Di Nucci, Giovanni Francesco Nicoletti, Rosa Salzillo, Alfredo De Rosa, Giuseppe Andrea Ferraro, Gianpaolo Papaccio, Vincenzo Desiderio, Virginia Tirino
Recent studies showed that mesenchymal stem cells derived from adipose tissue can promote tumour progression, raising some concerns regarding their use in regenerative medicine. In this context, we co-cultured either SAOS2 osteosarcoma or MCF7 breast cancer cells with human adipose stem cells (hASCs), in order to evaluate potential effects of cancer cells on hASCs differentiation, in vitro and in vivo. In this study we observed that both SAOS2 and MCF7 cell lines induced an increase in hASCs proliferation, compared to hASCs alone, but, surprisingly, neither changes in the expression of CD90, CD29, CD324 and vimentin, nor variations in the Twist and Slug mRNAs were detectable...
January 19, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28102674/2d-ir-spectroscopy-shows-that-optimised-dna-minor-groove-binding-of-hoechst33258-follows-an-induced-fit-model
#2
Lennart A I Ramakers, Gordon Hithell, John J May, Gregory M Greetham, Paul M Donaldson, Michael Towrie, Anthony W Parker, Glenn A Burley, Neil T Hunt
The induced fit binding model describes a conformational change occurring when a small molecule binds to its biomacromolecular target. The result is enhanced non-covalent interactions between ligand and biomolecule. Induced fit is well-established for small molecule-protein interactions, but its relevance to small molecule-DNA binding is less clear. We investigate the molecular determinants of Hoechst33258 binding to its preferred A-tract sequence relative to a sub-optimal alternating A-T sequence. Results from 2-dimensional infrared spectroscopy, which is sensitive to H-bonding and molecular structure changes, show that Hoechst33258 binding results in loss of minor groove spine of hydration in both sequences, but an additional perturbation of the base propeller twists occurs in the A-tract binding region...
January 19, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28101864/filamentous-structure-of-hard-%C3%AE-keratins-in-the-epidermal-appendages-of-birds-and-reptiles
#3
R D Bruce Fraser, David A D Parry
The structures of avian and reptilian epidermal appendages, such as feathers, claws and scales, have been modelled using X-ray diffraction and electron microscopy data, combined with sequence analyses. In most cases, a family of closely related molecules makes up the bulk of the appendage, and each of these molecules contains a central β-rich 34-residue segment, which has been identified as the principal component of the framework of the 3.4 nm diameter filaments. The N- and C-terminal segments form the matrix component of the filament/matrix complex...
2017: Sub-cellular Biochemistry
https://www.readbyqxmd.com/read/28100860/-a-pediatric-case-of-intractable-cerebrospinal-fluid-leakage-after-implantation-of-an-intrathecal-baclofen-pump
#4
Akitsugu Morishita, Hideo Aihara, Tomoaki Nakai, Masao Adachi
Intrathecal baclofen(ITB)infusion can offer a useful treatment for severe spasticity;however, numerous complications have been reported. We report a pediatric case in which intractable cerebrospinal fluid(CSF)leakage associated with several inconvenient symptoms arose after implantation of the ITB pump system. A 10-year-old girl with spastic quadriparesis and athetoid cerebral palsy underwent implantation of an ITB delivery system. After discharge, she presented with fluid collection surrounding the pump in the abdomen...
January 2017: No Shinkei Geka. Neurological Surgery
https://www.readbyqxmd.com/read/28100834/soft-robotic-sleeve-supports-heart-function
#5
Ellen T Roche, Markus A Horvath, Isaac Wamala, Ali Alazmani, Sang-Eun Song, William Whyte, Zurab Machaidze, Christopher J Payne, James C Weaver, Gregory Fishbein, Joseph Kuebler, Nikolay V Vasilyev, David J Mooney, Frank A Pigula, Conor J Walsh
There is much interest in form-fitting, low-modulus, implantable devices or soft robots that can mimic or assist in complex biological functions such as the contraction of heart muscle. We present a soft robotic sleeve that is implanted around the heart and actively compresses and twists to act as a cardiac ventricular assist device. The sleeve does not contact blood, obviating the need for anticoagulation therapy or blood thinners, and reduces complications with current ventricular assist devices, such as clotting and infection...
January 18, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28099797/epitaxy-induced-assembly-and-enantiomeric-switching-of-an-on-surface-formed-dinuclear-organocobalt-complex
#6
Raphael Hellwig, Tobias Paintner, Zhi Chen, Mario Ruben, Ari P Seitsonen, Florian Klappenberger, Harald Brune, Johannes V Barth
We report on the surface-guided synthesis of an unprecedented dinuclear organocobalt complex, its self-assembly into a complex nanoarchitecture, and a single-molecule level investigation of its switching behavior. Initially, an organic layer is prepared by depositing hexakis((trimethylsilyl)ethynyl)-benzene under ultra-high vacuum conditions onto Ag(111). After Co dosage at 200 Kelvin, low-temperature scanning tunneling microscopy (STM) reveals an epitaxy-mediated organization mechanism of molecules and on-surface formed host-guest complexes...
January 18, 2017: ACS Nano
https://www.readbyqxmd.com/read/28099017/absence-of-intramolecular-charge-transfer-with-4-fluoro-n-n-dimethylaniline-dma4f-contrary-to-an-experimental-report-supported-by-computations
#7
Klaas A Zachariasse, Attila Demeter, Sergey I Druzhinin
With 4-fluoro-N,N-dimethylaniline (DMA4F) only a single fluorescence from a locally excited (LE) state is observed, irrespective of solvent polarity, temperature, and excitation wavelength. The relatively small excited state dipole moment μe = 7.3 D confirms the identification as LE. The single exponential fluorescence decays in the nonpolar n-hexane (2.04 ns) and in the strongly polar acetonitrile (5.73 ns) are a further support. Similar results are obtained with 4-chloro-N,N-dimethylaniline (DMA4Cl), having a chlorobenzene subgroup, a somewhat better electron acceptor than the fluorobenzene moiety in DMA4F...
January 18, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28098875/exposure-to-tnf%C3%A2-%C3%AE-combined-with-tgf%C3%A2-%C3%AE-induces-carcinogenesis-in-vitro-via-nf-%C3%AE%C2%BAb-twist-axis
#8
Weilei Dong, Shuwen Sun, Xiaocheng Cao, Yinghong Cui, A Chen, Xiang Li, Jiansong Zhang, Jianguo Cao, Yifeng Wang
Persistent human papilloma virus (HPV) infection induces chronic inflammation resulting in human cervical cancer. However, the mechanisms underlying carcinogenesis via chronic inflammation remain largely unclear. We investigated the role of pro-inflammatory factors in epithelial-mesenchymal transition (EMT) and cancer stem cell-like (CSCL) characteristics of HeLa cells exposed to TNF‑α with or without TGF‑β. We then determined the role of NF-κB/Twist signal axis in the pathogenesis of cervical cancer...
January 16, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28098858/mir-345-inhibits-tumor-metastasis-and-emt-by-targeting-irf1-mediated-mtor-stat3-akt-pathway-in-hepatocellular-carcinoma
#9
Miao Yu, Huanzhou Xue, Yadong Wang, Quan Shen, Qingfeng Jiang, Xiao Zhang, Ke Li, Meng Jia, Jiangkun Jia, Jian Xu, Yuwei Tian
MicroRNAs (miRNAs) have been reported to play critical roles in tumor progression including hepatocellular carcinoma (HCC). Thus, the underlying mechanisms need further investigation. Previous study reported that loss of miR-345 expression indicated a poor prognosis of HCC patients. This study evaluated whether loss of miR-345 could promote the tumor metastasis and epithelial-mesenchymal-transition (EMT) of HCC by targeting interferon regulatory factor 1 (IRF1)-mediated mTOR/STAT3/AKT signaling. Underexpression of miR-345 was identified in 65 cases of human HCC compared to matched tumor-adjacent tissues by qRT-PCR...
January 17, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28096589/water-cooled-radiofrequency-neuroablation-for-sacroiliac-joint-dysfunctional-pain
#10
Binay Kumar Biswas, Samarjit Dey, Saumya Biswas, Varinder Kumar Mohan
Sacroiliac (SI) joint dysfunction is a common source of chronic low-back pain. Recent evidences from different parts of the world suggest that cooled radiofrequency (RF) neuroablation of sacral nerves supplying SI joints has superior pain alleviating properties than available existing treatment options for SI joint dysfunctional pain. A 35-year-old male had intractable bilateral SI joint pain (numeric rating scale [NRS] - 9/10) with poor treatment response to intra-articular steroid therapy. Bilateral water cooled = RF was applied for neuroablation of nerves supplying both SI joints...
October 2016: Journal of Anaesthesiology, Clinical Pharmacology
https://www.readbyqxmd.com/read/28096045/early-detection-of-left-ventricular-dysfunction-in-first-degree-relatives-of-diabetic-patients-by-myocardial-deformation-imaging-the-role-of-endothelial-glycocalyx-damage
#11
Ignatios Ikonomidis, George Pavlidis, Vaia Lambadiari, Fotini Kousathana, Maria Varoudi, Filio Spanoudi, Eirini Maratou, John Parissis, Helen Triantafyllidi, George Dimitriadis, John Lekakis
BACKGROUND: First-degree relatives of type-2 diabetes patients (FDR) present insulin resistance. We investigated whether FDR and dysglycaemic subjects demonstrate abnormal endothelial glycocalyx and LV deformation during postprandial hyperglycemia. METHODS: We studied 40 FDR with normal oral glucose test (OGTT), 40 subjects with abnormal OGTT (dysglycaemic) and 20 subjects with normal OGTT without parental history of diabetes (normoglycaemic). At 0 and 120min of OGTT we measured: a) LV longitudinal strain (LS) of subendocardial, mid-myocardial and subepicardial layers, global LS (GLS), peak twisting (pTw), untwisting velocity (pUtwVel), by speckle tracking echocardiography b) perfused boundary region (PBR) of the sublingual arterial microvessels; high PBR values represent reduced glycocalyx thickness...
January 8, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28095922/long-noncoding-rna-braveheart-promotes-cardiogenic-differentiation-of-mesenchymal-stem-cells-in-vitro
#12
Jingying Hou, Huibao Long, Changqing Zhou, Shaoxin Zheng, Hao Wu, Tianzhu Guo, Quanhua Wu, Tingting Zhong, Tong Wang
BACKGROUND: Mesenchymal stem cells (MSCs) have limited potential of cardiogenic differentiation. In this study, we investigated the influence of long noncoding RNA Braveheart (lncRNA-Bvht) on cardiogenic differentiation of MSCs in vitro. METHODS: MSCs were obtained from C57BL/6 mice and cultured in vitro. Cells were divided into three groups: blank control, null vector control, and lncRNA-Bvht. All three groups experienced exposure to hypoxia (1% O2) and serum deprivation for 24 h, and 24 h of reoxygenation (20% O2)...
January 17, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28095070/player-responses-to-match-and-training-demands-during-an-intensified-fixture-schedule-in-professional-rugby-league-a-case-study
#13
Craig Twist, Jamie Highton, Matthew Daniels, Nathan Mill, Graeme Close
Player loads and fatigue responses were reported in 15 professional rugby league players (24.3 ± 3.8 y) during a period of intensified fixtures. Repeated measures of internal and external loads, perceived wellbeing and jump flight time were recorded across 22 days, comprising nine training sessions and matches on days 5, 12, 15 and 21 (player exposure: 3.6 ± 0.6 matches). Mean training load (session RPE x duration) between matches was 1177, 1083, 103, and 650 AU, respectively. Relative distance in Match 1 (82 m/min) and Match 4 (79 m/min) was very likely lower in Match 2 (76 m/min) and likely higher in Match 3 (86 m/min)...
January 17, 2017: International Journal of Sports Physiology and Performance
https://www.readbyqxmd.com/read/28094887/exploring-the-influence-of-aggregation-on-the-structure-and-fluorescent-properties-of-a-tetraphenylethylene-derivative-a-theoretical-study
#14
Ying-Chen Duan, Yong Wu, Jun-Ling Jin, Dong-Mei Gu, Yun Geng, Min Zhang, Zhongmin Su
The case that aggregation brings a huge influence on the structure and fluorescent property of 5-(4-(1,2,2-triphenylvinyl)phenyl)thiophene-2-carbaldehyde (P4TA) was detailedly investigated here by employing both quantum mechanics and molecular mechanics. Besides the isolated molecule, the aggregated molecule both in water and crystal state was studied by focusing on the comparison of photoelectronic properties, including the geometrical and electronic structures at ground and excited states, emission and internal conversation properties...
January 17, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28093082/less-extrusion-debris-during-the-retreatment-of-curved-canals-using-twisted-files-with-higher-rotational-speeds-an-ex-vivo-study
#15
Mengdong Liu, Shijiang Xiong, Fei Tan, Yi Liu
BACKGROUND: Debris extrusion from the apical foramen can be problematic in severely curved canals. This study aimed to assess the use of Twisted Files (TF) at different rotational speeds during retreatment, compared with manual technique using Triple-Flex Files (TFF). METHODS: Forty-eight mesiobuccal root canals were randomized to four groups (n = 12 per group). In groups A, B, and C, gutta-percha was removed using TF at 500, 1000, and 1500 rpm, respectively, while it was removed using TFF in group D...
January 16, 2017: BMC Oral Health
https://www.readbyqxmd.com/read/28091828/liver-x-receptor-%C3%AE-lxr%C3%AE-promoted-invasion-and-emt-of-gastric-cancer-cells-by-regulation-of-nf-%C3%AE%C2%BAb-activity
#16
Linhua Ji, Bin Zhang, Gang Zhao
Aberrant expression of Liver X receptor α (LXRα) has been frequently reported in various types of cancers excluding gastric cancer (GC). Moreover, the role of LXRα in human GC has not been previously reported. In this study, we investigated the effect of LXRα down-regulation on invasion and EMT of GC. The expression of LXRα in GC cell lines was detected by real-time PCR. The LXRα siRNA was transiently transfected into GC cells using Lipofectamine™ 2000 reagent. Subsequently, cell invasive ability was evaluated by Transwell assays...
January 16, 2017: Human Cell
https://www.readbyqxmd.com/read/28091620/role-of-molecular-conformations-in-rubrene-polycrystalline-films-growth-from-vacuum-deposition-at-various-substrate-temperatures
#17
Ku-Yen Lin, Yan-Jun Wang, Ko-Lun Chen, Ching-Yuan Ho, Chun-Chuen Yang, Ji-Lin Shen, Kuan-Cheng Chiu
We report on the optical and structural characterization of rubrene polycrystalline films fabricated from vacuum deposition with various substrate temperatures (Tsub). Depending on Tsub, the role of twisted and planar rubrene conformational isomers on the properties of rubrene films is focused. The temperature (T)-dependent inverse optical transmission (IOT) and photoluminescence (PL) spectra were performed on these rubrene films. The origins of these IOT and PL peaks are explained in terms of the features from twisted and planar rubrene molecules and of the band characteristics from rubrene molecular solid films...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28091427/evaluation-of-surface-characteristics-of-rotary-nickel-titanium-instruments-produced-by-different-manufacturing-methods
#18
U Inan, M Gurel
BACKGROUND: Instrument fracture is a serious concern in endodontic practice. OBJECTIVE: The aim of this study was to investigate the surface quality of new and used rotary nickel-titanium (NiTi) instruments manufactured by the traditional grinding process and twisting methods. MATERIALS AND METHODS: Total 16 instruments of two rotary NiTi systems were used in this study. Eight Twisted Files (TF) (SybronEndo, Orange, CA, USA) and 8 Mtwo (VDW, Munich, Germany) instruments were evaluated...
February 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/28089831/monomethyltransferase-setd8-regulates-breast-cancer-metabolism-via-stabilizing-hypoxia-inducible-factor-1%C3%AE
#19
Run Huang, Yang Yu, Xiangyun Zong, Xiaolin Li, Lisi Ma, Qi Zheng
SETD8 is a methyltransferase that specifically catalyzes the monomethylation of lysine 20 on histone H4. Previous studies have demonstrated that SETD8 is associated with proper cell cycle progression, DNA damage response, and transcriptional regulation. A recent study revealed that SETD8 played an important role in epithelial-mesenchymal transition (EMT) in association with TWIST and enhanced metastatic potential of breast cancer cells. However, the contribution of SETD8 to metabolism reprogramming, one hallmark of cancer, has never been reported...
January 13, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28089518/transcriptional-pre-patterning-of-drosophila-gastrulation
#20
Bomyi Lim, Michael Levine, Yuji Yamakazi
Gastrulation of the Drosophila embryo is one of the most intensively studied morphogenetic processes in animal development [1-4]. Particular efforts have focused on the formation of the ventral furrow, whereby ∼1,000 presumptive mesoderm cells exhibit coordinated apical constrictions that mediate invagination [5, 6]. Apical constriction depends on a Rho GTPase signaling pathway (T48/Fog) that is deployed by the developmental regulatory genes twist and snail [7-10]. It is thought that coordinate mesoderm constriction depends on high levels of myosin along the ventral midline, although the basis for this localization is uncertain...
January 9, 2017: Current Biology: CB
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