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Micropropagation

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https://www.readbyqxmd.com/read/27889521/azacytidine-and-mir156-promote-rooting-in-adult-but-not-in-juvenile-arabidopsis-tissues
#1
Mehdi Massoumi, Frans A Krens, Richard G F Visser, Geert-Jan M De Klerk
Poor adventitious root (AR) formation is a major obstacle in micropropagation and conventional vegetative propagation of many crops. It is affected by many endogenous and exogenous factors. With respect to endogenous factors, the phase change from juvenile to adult has a major influence on AR formation and rooting is usually much reduced or even fully inhibited in adult tissues. It has been reported that the phase change is characterized by an increase in DNA-methylation and a decrease in the expression of microRNA156 (miR156)...
November 15, 2016: Journal of Plant Physiology
https://www.readbyqxmd.com/read/27866249/investigation-of-endophytic-bacterial-community-in-supposedly-axenic-cultures-of-pineapple-and-orchids-with-evidence-on-abundant-intracellular-bacteria
#2
Natalia Pimentel Esposito-Polesi, Monita Fiori de Abreu-Tarazi, Cristina Vieira de Almeida, Siu Mui Tsai, Marcílio de Almeida
Asepsis, defined as the absence of microbial contamination, is one of the most important requirements of plant micropropagation. In long-term micropropagated cultures, there may occasionally occur scattered microorganism growth in the culture medium. These microorganisms are common plant components and are known as latent endophytes. Thus, the aim of this research was to investigate the presence of endophytic bacteria in asymptomatic pineapple and orchid microplants, which were cultivated in three laboratories for 1 year...
November 19, 2016: Current Microbiology
https://www.readbyqxmd.com/read/27819136/low-humic-acids-promote-in-vitro-lily-bulblet-enlargement-by-enhancing-roots-growth-and-carbohydrate-metabolism
#3
Yun Wu, Yi-Ping Xia, Jia-Ping Zhang, Fang Du, Lin Zhang, Yi-di Ma, Hong Zhou
Bulblet development is a problem in global lily bulb production and carbohydrate metabolism is a crucial factor. Micropropagation acts as an efficient substitute for faster propagation and can provide a controllable condition to explore bulb growth. The present study was conducted to investigate the effects of humic acid (HA) on bulblet swelling and the carbohydrate metabolic pathway in Lilium Oriental Hybrids 'Sorbonne' under in vitro conditions. HA greatly promoted bulblet growth at 0.2, 2.0, and 20.0 mg/L, and pronounced increases in bulblet sucrose, total soluble sugar, and starch content were observed for higher HA concentrations (≥2...
2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27815856/organic-amendments-enhance-pb-tolerance-and-accumulation-during-micropropagation-of-daphne-jasminea
#4
Alina Wiszniewska, Ewa Muszyńska, Ewa Hanus-Fajerska, Sylwester Smoleń, Michał Dziurka, Kinga Dziurka
The study investigated the effects of organic amendments: pineapple pulp (PP) and agar hydrolyzate (AH), on micropropagation and Pb bioaccumulation and tolerance in a woody shrub Daphne jasminea cultured in vitro. The amendments were analyzed for their content of carbohydrates, phenolic acids, and phytohormones and added at a dose of 10 mL L(-1) to the medium containing 1.0 mM lead nitrate. Micropropagation coefficient increased by 10.2-16.6 % in PP and AH variants, respectively. Growth tolerance index increased by 22...
November 5, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27807436/artificial-neural-network-genetic-algorithm-as-powerful-tool-to-predict-and-optimize-in-vitro-proliferation-mineral-medium-for-g-%C3%A3-n15-rootstock
#5
Mohammad M Arab, Abbas Yadollahi, Abdolali Shojaeiyan, Hamed Ahmadi
One of the major obstacles to the micropropagation of Prunus rootstocks has, up until now, been the lack of a suitable tissue culture medium. Therefore, reformulation of culture media or modification of the mineral content might be a breakthrough to improve in vitro multiplication of G × N15 (garnem). We found artificial neural network in combination of genetic algorithm (ANN-GA) as a very precise and powerful modeling system for optimizing the culture medium, So that modeling the effects of MS mineral salts ([Formula: see text], [Formula: see text], [Formula: see text], Ca(2+), K(+), [Formula: see text], Mg(2+), and Cl(-)) on in vitro multiplication parameters (the number of microshoots per explant, average length of microshoots, weight of calluses derived from the base of stem explants, and quality index of plantlets) of G × N15...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27761076/elicitation-of-phenolics-from-the-micropropagated-endangered-medicinal-plant-calligonum-polygonoides-l-polygonoaceae
#6
Asmaa I Owis, Nada S Abdelwahab, Adel A Abul-Soad
BACKGROUND: Calligonum polygonoides L. subsp. comosum (L'Hér.) Sosk. is a plant species belonging to family Polygonaceae. Susceptibility to threaten, presence of various chemical constituents, and many medicinal effects reported for this plant in addition to rareness of in vitro culture studies have fuelled the need for its micropropagation and phytochemical investigations of the produced cultures. OBJECTIVES: To employ in vitro culture technique for ex situ conservation of C...
July 2016: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/27746694/in%C3%A2-vitro-grown-thickened-taproots-a-new-type-of-soil-transplanting-source-in-panax-ginseng
#7
Jong Youn Kim, Dong Hwi Kim, Young Chang Kim, Kee Hong Kim, Jung Yeon Han, Yong Eui Choi
BACKGROUND: The low survival rate of in vitro regenerated Panax ginseng plantlets after transfer to soil is the main obstacle for their successful micropropagation and molecular breeding. In most cases, young plantlets converted from somatic embryos are transferred to soil. METHODS: In vitro thickened taproots, which were produced after prolonged culture of ginseng plantlets, were transferred to soil. RESULTS: Taproot thickening of plantlets occurred near hypocotyl and primary roots...
October 2016: Journal of Ginseng Research
https://www.readbyqxmd.com/read/27746667/in-vitro-propagation-and-assessment-of-genetic-stability-of-acclimated-plantlets-of-cornus-alba-l-using-rapd-and-issr-markers
#8
Agnieszka Ilczuk, Ewelina Jacygrad
Cornus alba L. (white dogwood) is an important ornamental shrub having a wide range of applications such as reforestation programs and soil retention systems. The vegetative propagation of dogwood by cuttings may be slow, difficult, and cultivar dependent; therefore, an improved micropropagation method was developed. Nodal stem segments of C. alba cultivars 'Aurea' and 'Elegantissima' were cultured on media enriched with six different sources of macronutrients. Media were supplemented with either N(6)-benzyladenine (BA) or thidiazuron (TDZ) in combination with 1-naphthaleneacetic acid (NAA)...
2016: In Vitro Cellular & Developmental Biology. Plant: Journal of the Tissue Culture Association
https://www.readbyqxmd.com/read/27630052/preculturing-effect-of-thidiazuron-on-in-vitro-shoot-multiplication-and-micropropagation-round-in-capparis-decidua-forsk-an-important-multipurpose-plant
#9
Najat A W Bukhari, Iram Siddique, Kahkashan Perveen
An efficient protocol was developed for clonal multiplication of an important shrub: Capparis decidua (Forsk.) Edgew, through in vitro shoot induction and multiplication from nodal explants. Pretreatment of nodal explants in a liquid Murashige and Skoog (MS) medium augmented with various thidiazuron (TDZ) concentrations at relatively high levels (5-100 μM) for different time duration (4, 8, 12 and 16 d), proved a significant approach for in vitro shoot production. After an initial exposure time to TDZ, nodal explants were inoculated onto a MS basal medium devoid of TDZ for further induction and proliferation...
September 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27590059/physiological-differences-and-changes-in-global-dna-methylation-levels-in-agave-angustifolia-haw-albino-variant-somaclones-during-the-micropropagation-process
#10
Fátima Duarte-Aké, Eduardo Castillo-Castro, Felipe Barredo Pool, Francisco Espadas, Jorge M Santamaría, Manuel L Robert, Clelia De-la-Peña
Global DNA methylation changes caused by in vitro conditions are associated with the subculturing and phenotypic variation in Agave angustifolia Haw. While the relationship between the development of albinism and in vitro culture is well documented, the role of epigenetic processes in this development leaves some important questions unanswered. During the micropropagation of Agave angustifolia Haw., we found three different phenotypes, green (G), variegated (V) and albino (A). To understand the physiological and epigenetic differences among the somaclones, we analyzed several morphophysiological parameters and changes in the DNA methylation patterns in the three phenotypes during their in vitro development...
September 2, 2016: Plant Cell Reports
https://www.readbyqxmd.com/read/27553812/micropropagation-and-genetic-transformation-of-tylophora-indica-burm-f-merr-a-review
#11
Jaime A Teixeira da Silva, Sumita Jha
This review provides an in-depth and comprehensive overview of the in vitro culture of Tylophora species, which have medicinal properties. Tylophora indica (Burm. f.) Merr. is a climbing perennial vine with medicinal properties. The tissue culture and genetic transformation of T. indica, which has been extensively studied, is reviewed. Micropropagation using nodal explants has been reported in 25 % of all publications. Leaf explants from field-grown plants has been the explant of choice of independent research groups, which reported direct and callus-mediated organogenesis as well as callus-mediated somatic embryogenesis...
November 2016: Plant Cell Reports
https://www.readbyqxmd.com/read/27486441/analysis-of-plant-growth-promoting-effects-of-fluorescent-pseudomonas-strains-isolated-from-mentha-piperita-rhizosphere-and-effects-of-their-volatile-organic-compounds-on-essential-oil-composition
#12
Maricel V Santoro, Pablo C Bogino, Natalia Nocelli, Lorena Del Rosario Cappellari, Walter F Giordano, Erika Banchio
Many species or strains of the genus Pseudomonas have been characterized as plant growth promoting rhizobacteria (PGPR). We used a combination of phenotypic and genotypic techniques to analyze the community of fluorescent Pseudomonas strains in the rhizosphere of commercially grown Mentha piperita (peppermint). Biochemical techniques, Amplified rDNA Restriction Analysis (ARDRA), and 16S rRNA gene sequence analysis revealed that the majority of the isolated native fluorescent strains were P. putida. Use of two Repetitive Sequence-based PCR (rep-PCR) techniques, BOX-PCR and ERIC-PCR, allowed us to evaluate diversity among the native strains and to more effectively distinguish among them...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27478496/conservation-and-sustainable-use-of-medicinal-plants-problems-progress-and-prospects
#13
Shi-Lin Chen, Hua Yu, Hong-Mei Luo, Qiong Wu, Chun-Fang Li, André Steinmetz
Medicinal plants are globally valuable sources of herbal products, and they are disappearing at a high speed. This article reviews global trends, developments and prospects for the strategies and methodologies concerning the conservation and sustainable use of medicinal plant resources to provide a reliable reference for the conservation and sustainable use of medicinal plants. We emphasized that both conservation strategies (e.g. in situ and ex situ conservation and cultivation practices) and resource management (e...
2016: Chinese Medicine
https://www.readbyqxmd.com/read/27436921/retraction-note-to-influencing-micropropagation-in-clitoria-ternatea-l-through-the-manipulation-of-tdz-levels-and-use-of-different-explant-types
#14
Seemab Mukhtar, Naseem Ahmad, Md Imran Khan, Mohammad Anis, Ibrahim M Aref
[This retracts the article DOI: 10.1007/s12298-012-0136-4.].
April 2016: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/27434060/genetic-homogeneity-revealed-using-scot-issr-and-rapd-markers-in-micropropagated-pittosporum-eriocarpum-royle-an-endemic-and-endangered-medicinal-plant
#15
Julie Thakur, Mayank D Dwivedi, Pragya Sourabh, Prem L Uniyal, Arun K Pandey
Pittosporum eriocarpum Royle, a medicinally important taxon, is endemic to Uttarakhand region of Himalaya. It has become endangered due to over-collection and the loss of habitats. As raising plants through seeds in this plant is problematic, a reliable protocol for micropropagation using nodal explants has been developed. High shoot regeneration (95%) occurred in MS medium augmented with BA 0.4mg/l in combination IBA 0.6mg/l. In vitro regenerated shoots were rooted in MS medium supplemented with three auxins, of which 0...
2016: PloS One
https://www.readbyqxmd.com/read/27356371/-study-on-rapid-micropropagation-in-vitro-technique-of-guangfeng-medicinal-yam-dioscorea-opposita-plantlets
#16
Ming-Hua Yin, Zhi-Jian Xu, Sheng-qin Zhang, Si-Jie Lv, Yan-hong Zeng, You-zhang Fu, Sen-rong Hong
OBJECTIVE: In order to provide methodology reference for virus-free and germplasm conservation of Guangfeng medicinal yam (Dioscorea opposita) plantlets, rapid micropropagation in vitro technique of Guangfeng medicinal yam plantlets was studied. METHODS: Using the method of plant tissue culture, single factor test and flow-cytometry, the basic procedure of Guangfeng medicinal yam tissue culture was established and the DNA content of Guangfeng medicinal yam plantlets and its potted seedlings was detected...
November 2015: Zhong Yao Cai, Zhongyaocai, Journal of Chinese Medicinal Materials
https://www.readbyqxmd.com/read/27318708/detection-of-variation-in-long-term-micropropagated-mature-pistachio-via-dna-based-molecular-markers
#17
Hülya Akdemir, Veysel Suzerer, Engin Tilkat, Ahmet Onay, Yelda Ozden Çiftçi
Determination of genetic stability of in vitro-grown plantlets is needed for safe and large-scale production of mature trees. In this study, genetic variation of long-term micropropagated mature pistachio developed through direct shoot bud regeneration using apical buds (protocol A) and in vitro-derived leaves (protocol B) was assessed via DNA-based molecular markers. Randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) were employed, and the obtained PIC values from RAPD (0...
December 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27314516/physiological-characterization-and-true-to-typeness-evaluation-of-in%C3%A2-vitro-and-ex%C3%A2-vitro-seedlings-of-pinus-elliottii-a-contribution-to-breeding-programs
#18
Sandra Nunes, Conceição Santos, José Moutinho-Pereira, Carlos Correia, Helena Oliveira, José Miguel Ferreira de Oliveira, Vanessa Tolentino Pereira, Tânia Almeida, Liliana Marum, Maria Celeste Dias
Pinus elliottii var. elliottii is a pine species with enormous economic value particularly for timber and resin industries, and is subject of high pressure for genetic improvement and cloning elite genotypes. We have recently developed a robust micropropagation protocol for this species. Plantlets performance needs to be evaluated to validate this protocol for further mass propagation. Micropropagated plantlets and seed-derived plants with similar age and shoot length were compared regarding photosynthesis, carbohydrates and pigments content, water status, DNA content and cell cycle dynamics...
October 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27307203/investigating-the-effect-of-cadmium-and-aluminium-on-growth-and-stress-induced-responses-in-the-micropropagated-medicinal-plant-hypoxis-hemerocallidea
#19
A Okem, M Moyo, W A Stirk, J F Finnie, J Van Staden
Hypoxis hemerocallidea is a highly utilized medicinal plant in South Africa. Its cultivation has received considerable attention in order to meet the high demand. High levels of cadmium (Cd) and aluminum (Al) in H. hemerocallidea plants sold in traditional medicinal markets was previously reported. The present study used an in vitro propagation model to investigate the uptake of Cd and Al by H. hemerocallidea and their effect on plant growth, elemental uptake and some stress-induced responses such as pigment, malondialdehyde (MDA), proline content and ultrastructural changes...
September 2016: Plant Biology
https://www.readbyqxmd.com/read/27259554/low-vapour-pressure-deficit-affects-nitrogen-nutrition-and-foliar-metabolites-in-silver-birch
#20
Jenna Lihavainen, Viivi Ahonen, Sarita Keski-Saari, Sari Kontunen-Soppela, Elina Oksanen, Markku Keinänen
Air humidity indicated as vapour pressure deficit (VPD) is directly related to transpiration and stomatal function of plants. We studied the effects of VPD and nitrogen (N) supply on leaf metabolites, plant growth, and mineral nutrition with young micropropagated silver birches (Betula pendula Roth.) in a growth chamber experiment. Plants that were grown under low VPD for 26 d had higher biomass, larger stem diameter, more leaves, fewer fallen leaves, and larger total leaf area than plants that were grown under high VPD...
July 2016: Journal of Experimental Botany
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