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https://www.readbyqxmd.com/read/29138859/17%C3%AE-estradiol-attenuates-ovariectomy%C3%A2-induced-bone-deterioration-through-the-suppression-of-the-epha2-ephrina2-signaling-pathway
#1
Lianyong Liu, Lin Zhou, Xiaorong Yang, Qi Liu, Ling Yang, Chao Zheng, Yongling Zhao, Zhenlin Zhang, Xiaohong Luo
The present study aimed to investigate whether 17β‑estradiol (E2) exerts protective effects on bone deterioration induced by ovariectomy (OVX) through the ephA2/ephrinA2 signaling pathway in rats. Female rats were subjected to OVX, sham surgeryor OVX+E2 treatment. Levels of biomarkers were measured in serum and urine. Hematoxylin and eosin staining was performed on paraffin‑embedded bone sections. Expression of genes and proteins was analyzed by reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively...
November 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29117218/compatible-bacterial-mixture-tolerant-to-desiccation-improves-maize-plant-growth
#2
Dalia Molina-Romero, Antonino Baez, Verónica Quintero-Hernández, Miguel Castañeda-Lucio, Luis Ernesto Fuentes-Ramírez, María Del Rocío Bustillos-Cristales, Osvaldo Rodríguez-Andrade, Yolanda Elizabeth Morales-García, Antonio Munive, Jesús Muñoz-Rojas
Plant growth-promoting rhizobacteria (PGPR) increase plant growth and crop productivity. The inoculation of plants with a bacterial mixture (consortium) apparently provides greater benefits to plant growth than inoculation with a single bacterial strain. In the present work, a bacterial consortium was formulated containing four compatible and desiccation-tolerant strains with potential as PGPR. The formulation had one moderately (Pseudomonas putida KT2440) and three highly desiccation-tolerant (Sphingomonas sp...
2017: PloS One
https://www.readbyqxmd.com/read/29040309/targeted-pth4-expressing-cell-ablation-impairs-skeletal-mineralization-in-zebrafish
#3
Paula Suarez-Bregua, Ankur Saxena, Marianne E Bronner, Josep Rotllant
Skeletal development and mineralization are essential processes driven by the coordinated action of neural signals, circulating molecules and local factors. Our previous studies revealed that the novel neuropeptide Pth4, synthesized by hypothalamic cells, was involved in bone metabolism via phosphate regulation in adult zebrafish. Here, we investigate the role of pth4 during skeletal development using single-cell resolution, two-photon laser ablation of Pth4:eGFP-expressing cells and confocal imaging in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/29029772/inhibition-of-mammalian-target-of-rapamycin-signaling-with-rapamycin-prevents-trauma-induced-heterotopic-ossification
#4
Ammar T Qureshi, Devaveena Dey, Erin M Sanders, Jonathan G Seavey, Allison M Tomasino, Kaitlyn Moss, Benjamin Wheatley, David Cholok, Shawn Loder, John Li, Benjamin Levi, Thomas A Davis
A pressing clinical need exists for 63% to 65% of combat-wounded service members and 11% to 20% of civilians who develop heterotopic ossification (HO) after blast-related extremity injury and traumatic injuries, respectively. The mammalian target of rapamycin pathway is a central cellular sensor of injury. We evaluated the prophylactic effects of rapamycin, a selective inhibitor of mammalian target of rapamycin signaling, on HO formation in a rat model of blast-related, polytraumatic extremity injury. Rapamycin was administered intraperitoneally daily for 14 days at 0...
November 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28990080/rapamycin-promotes-osteogenesis-under-inflammatory-conditions
#5
Xing Li, Bei Chang, Banchao Wang, Wenhuan Bu, Liang Zhao, Jie Liu, Lin Meng, Lu Wang, Ying Xin, Dandan Wang, Qi Tang, Changyu Zheng, Hongchen Sun
Chronic periodontitis, a common oral disease, usually results in irreversible bone resorption. Bone regeneration is a complex process between bone‑forming activity of osteoblasts and bone‑resorbing activity of osteoclasts, and still remains a challenge for physicians clinically. A previous study demonstrated that the mechanistic target of rapamycin signaling pathway is involved in osteogenic differentiation of mesenchymal stromal cells. Herein, whether rapamycin could be used to induce osteogenic differentiation of primary bone marrow‑derived mesenchymal stem cells (BMSCs) in vitro and promote new bone formation in vivo were evaluated...
December 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28955517/aff1-and-aff4-differentially-regulate-the-osteogenic-differentiation-of-human-mscs
#6
Chen-Chen Zhou, Qiu-Chan Xiong, Xin-Xing Zhu, Wen Du, Peng Deng, Xiao-Bing Li, Yi-Zhou Jiang, Shu-Juan Zou, Cun-Yu Wang, Quan Yuan
AFF1 and AFF4 belong to the AFF (AF4/FMR2) family of proteins, which function as scaffolding proteins linking two different transcription elongation factors, positive elongation factor b (P-TEFb) and ELL1/2, in super elongation complexes (SECs). Both AFF1 and AFF4 regulate gene transcription through elongation and chromatin remodeling. However, their function in the osteogenic differentiation of mesenchymal stem cells (MSCs) is unknown. In this study, we show that small interfering RNA (siRNA)-mediated depletion of AFF1 in human MSCs leads to increased alkaline phosphatase (ALP) activity, enhanced mineralization and upregulated expression of osteogenic-related genes...
2017: Bone Research
https://www.readbyqxmd.com/read/28899668/the-sp7-gene-is-required-for-maturation-of-osteoblast-lineage-cells-in-medaka-oryzias-latipes-vertebral-column-development
#7
Yuki Azetsu, Keiji Inohaya, Yoshiro Takano, Masato Kinoshita, Mai Tasaki, Akira Kudo
Sp7 is a zinc finger transcription factor that is essential for osteoblast differentiation in mammals. To verify the characteristic features of osteoblast-lineage cells in teleosts, we established medaka sp7 mutants using a transcription activator-like effector nuclease (TALEN) genome editing system. These mutants showed severe defects in the formation of skeletal structures. In particular, the neural and the hemal arches were not formed, although the chordal centra were formed. Analysis of the transgenic medaka revealed that sp7 mutant had normal distribution of type X collagen a1 a (col10a1a)-positive osteoblast-like cells around the centrum and at the proximal region of the vertebral arch...
November 15, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28853173/pharmacological-inhibition-of-s6k1-impairs-self-renewal-and-osteogenic-differentiation-of-bone-marrow-stromal-cells
#8
Xiaohui Gu, Xuejie Fu, Jihang Lu, Saijilafu, Bin Li, Zong-Ping Luo, Jianquan Chen
mTORC1 signaling not only plays important physiological roles in the regulation of proliferation and osteogenic differentiation of BMSCs, but also mediates exogenous Wnt-induced protein anabolism and osteoblast differentiation. However, the downstream effectors of the mTORC1 signaling in the above processes are still poorly understood. In this study, we explored the specific role of S6K1, one of the major targets of the mTORC1 pathway, in BMSCs self-renewal and osteogenic differentiation. We first found that S6K1 was active in primary mouse bone marrow stromal cells, and further activated upon osteogenic induction...
July 11, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28821457/spontaneous-mutation-of-dock7-results-in-lower-trabecular-bone-mass-and-impaired-periosteal-expansion-in-aged-female-misty-mice
#9
Phuong T Le, Kathleen A Bishop, David E Maridas, Katherine J Motyl, Daniel J Brooks, Kenichi Nagano, Roland Baron, Mary L Bouxsein, Clifford J Rosen
Misty mice (m/m) have a loss of function mutation in Dock7 gene, a guanine nucleotide exchange factor, resulting in low bone mineral density, uncoupled bone remodeling and reduced bone formation. Dock7 has been identified as a modulator of osteoblast number and in vitro osteogenic differentiation in calvarial osteoblast culture. In addition, m/m exhibit reduced preformed brown adipose tissue innervation and temperature as well as compensatory increase in beige adipocyte markers. While the low bone mineral density phenotype is in part due to higher sympathetic nervous system (SNS) drive in young mice, it is unclear what effect aging would have in mice homozygous for the mutation in the Dock7 gene...
December 2017: Bone
https://www.readbyqxmd.com/read/28777372/p53-inhibits-sp7-osterix-activity-in-the-transcriptional-program-of-osteoblast-differentiation
#10
Natalia Artigas, Beatriz Gámez, Mónica Cubillos-Rojas, Cristina Sánchez-de Diego, José Antonio Valer, Gabriel Pons, José Luis Rosa, Francesc Ventura
Osteoblast differentiation is achieved by activating a transcriptional network in which Dlx5, Runx2 and Osx/SP7 have fundamental roles. The tumour suppressor p53 exerts a repressive effect on bone development and remodelling through an unknown mechanism that inhibits the osteoblast differentiation programme. Here we report a physical and functional interaction between Osx and p53 gene products. Physical interaction was found between overexpressed proteins and involved a region adjacent to the OSX zinc fingers and the DNA-binding domain of p53...
December 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28746051/stanozolol-soaked-grafts-enhance-new-bone-formation-in-rat-calvarial-critical-size-defects
#11
G Ghiacci, G Graiani, A Cacchioli, C Galli, S Lumetti, F Ravanetti, L Elviri, E Manfredi, G M Macaluso, R Sala
Androgen hormones play a significant role in regulating bone morphogenesis and in maintaining bone homeostasis throughout life. This study aimed to investigate the local effects of the non-aromatizable androgen stanozolol (ST) on bone regeneration in rats. Bilateral critical-size defects were created in the parietal bone of 26 male Wistar rats: the defect on one side was filled with a deproteinized bovine bone scaffold (DBB) soaked in ST solution (test) and the contralateral with DBB alone (control). Samples were collected at one month and three months...
July 26, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28731788/identification-of-osr2-transcriptional-target-genes-in-palate-development
#12
X Fu, J Xu, P Chaturvedi, H Liu, R Jiang, Y Lan
Previous studies have identified the odd-skipped related 2 (Osr2) transcription factor as a key intrinsic regulator of palatal shelf growth and morphogenesis. However, little is known about the molecular program acting downstream of Osr2 in the regulation of palatogenesis. In this study, we isolated palatal mesenchyme cells from embryonic day 12.5 (E12.5) and E13.5 Osr2(RFP/+) and Osr2(RFP/-) mutant mouse embryos and performed whole transcriptome RNA sequencing analyses. Differential expression analysis of the RNA sequencing datasets revealed that expression of 70 genes was upregulated and expression of 61 genes was downregulated by >1...
November 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28720797/commensal-gut-microbiota-immunomodulatory-actions-in-bone-marrow-and-liver-have-catabolic-effects-on-skeletal-homeostasis-in-health
#13
Chad M Novince, Carolyn R Whittow, Johannes D Aartun, Jessica D Hathaway, Nicole Poulides, Michael B Chavez, Heidi M Steinkamp, Kaeleigh A Kirkwood, Emily Huang, Caroline Westwater, Keith L Kirkwood
Despite knowledge the gut microbiota regulates bone mass, mechanisms governing the normal gut microbiota's osteoimmunomodulatory effects on skeletal remodeling and homeostasis are unclear in the healthy adult skeleton. Young adult specific-pathogen-free and germ-free mice were used to delineate the commensal microbiota's immunoregulatory effects on osteoblastogenesis, osteoclastogenesis, marrow T-cell hematopoiesis, and extra-skeletal endocrine organ function. We report the commensal microbiota has anti-anabolic effects suppressing osteoblastogenesis and pro-catabolic effects enhancing osteoclastogenesis, which drive bone loss in health...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28719571/mir-27b-3p-suppressed-osteogenic-differentiation-of-maxillary-sinus-membrane-stem-cells-by-targeting-sp7
#14
Wei Peng, Shuangxi Zhu, Xiang Li, Junquan Weng, Songling Chen
OBJECTIVE: To explore the critical role and function of miRNAs in the regulation of development and physiology of maxillary sinus membrane stem cell (MSMSC) osteogenesis. METHODS: Microarray analysis was performed to screen the miRNAs expression profiles during the process of MSMSC osteogenic differentiation. Quantitative real-time polymerase chain reaction was applied to verify the miRNAs expression profiles. Gain- and loss-of-function experiments were used to demonstrate that miR-27b-3p inhibited MSMSC osteoblastic differentiation...
August 2017: Implant Dentistry
https://www.readbyqxmd.com/read/28711791/labeled-azospirillum-brasilense-wild-type-and-excretion-ammonium-strains-in-association-with-barley-roots
#15
Adrian Richard Schenberger Santos, Rafael Mazer Etto, Rafaela Wiegand Furmam, Denis Leandro de Freitas, Karina Freire d'Eça Nogueira Santos, Emanuel Maltempi de Souza, Fábio de Oliveira Pedrosa, Ricardo Antônio Ayub, Maria Berenice Reynaud Steffens, Carolina Weigert Galvão
Soil bacteria colonization in plants is a complex process, which involves interaction between many bacterial characters and plant responses. In this work, we labeled Azospirillum brasilense FP2 (wild type) and HM053 (excretion-ammonium) strains by insertion of the reporter gene gusA-kanamycin into the dinitrogenase reductase coding gene, nifH, and evaluated bacteria colonization in barley (Hordeum vulgare). In addition, we determined inoculation effect based on growth promotion parameters. We report an uncommon endophytic behavior of A...
July 4, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28574187/menopause-transition-promotes-distinct-modulation-of-mrnas-and-mirnas-expression-in-calvaria-and-bone-marrow-osteoblastic-cells
#16
Mayara Sgarbi Semeghini, Fernanda Grilo de Azevedo, Roger Rodrigo Fernandes, Amanda Freire Assis, Janaína Andrea Dernowsek, Adalberto Luiz Rosa, Selma Siéssere, Geraldo Aleixo Passos, Karina Fittipaldi Bombonato-Prado
Investigation on functional genome research may contribute to the knowledge of functional roles of different mRNAs and miRNAs in bone cells of osteoporotic animals. Currently, few studies indicate the changes in gene modulation that osteoporosis causes in osteoblastic cells from different sites. Thus, the purpose of this investigation was to evaluate cell viability, alkaline phosphatase activity and modulation of mRNAs/miRNAs in osteoblastic cells from calvaria and bone marrow by means of microarray technology...
June 2, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28439037/regulation-of-a-glycerol-induced-quinoprotein-alcohol-dehydrogenase-by-%C3%AF-54-and-a-luxr-type-regulator-in-azospirillum-brasilense-sp7
#17
Vijay Shankar Singh, Ashutosh Prakash Dubey, Ankush Gupta, Sudhir Singh, Bhupendra Narain Singh, Anil Kumar Tripathi
Azospirillum brasilense Sp7 uses glycerol as a carbon source for growth and nitrogen fixation. When grown in medium containing glycerol as a source of carbon, it upregulates the expression of a protein which was identified as quinoprotein alcohol dehydrogenase (ExaA). Inactivation of exaA adversely affects the growth of A. brasilense on glycerol. A determination of the transcription start site of exaA revealed an RpoN-dependent -12/-24 promoter consensus. The expression of an exaA::lacZ fusion was induced maximally by glycerol and was dependent on σ(54) Bioinformatic analysis of the sequence flanking the -12/-24 promoter revealed a 17-bp sequence motif with a dyad symmetry of 6 nucleotides upstream of the promoter, the disruption of which caused a drastic reduction in promoter activity...
July 1, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28437476/clc-3-chloride-channel-mediates-the-role-of-parathyroid-hormone-1-34-on-osteogenic-differentiation-of-osteoblasts
#18
Xiaolin Lu, Yin Ding, Qiannan Niu, Shijie Xuan, Yan Yang, Yulong Jin, Huan Wang
INTRODUCTION: Different concentrations of parathyroid hormone [1-34] (PTH [1-34]) can have totally opposite effects on osteoblasts. Intermittent stimulation with PTH can significantly increase bone mineral density in vitro, mainly through the protein kinase A (PKA) signaling pathway, which phosphorylates runt-related transcription factor 2 (Runx2). The ClC-3 chloride channel, an important anion channel, can also promote osteogenesis via the Runx2 pathway based on recent studies. The purpose of our study, therefore, is to research whether the ClC-3 chloride channel has an effect on PTH osteodifferentiation in MC3T3-E1 cells...
2017: PloS One
https://www.readbyqxmd.com/read/28425950/diversity-analysis-and-bioresource-characterization-of-halophilic-bacteria-isolated-from-a-south-african-saltpan
#19
Ramganesh Selvarajan, Timothy Sibanda, Memory Tekere, Hlengilizwe Nyoni, Stephen Meddows-Taylor
Though intensive research has been channeled towards the biotechnological applications of halophiles and other extremophilic microbes, these studies have not been, by any means, exhaustive. Saline environments still offer a vast diversity of microbes with potential to produce an array of natural products which can only be unlocked by concerted research efforts. In this study, a combination of culture and molecular approaches were employed to characterize halophilic bacteria from saltpan water samples and profile their potential biotechnological applications...
April 20, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28416707/distinct-domains-of-chea-confer-unique-functions-in-chemotaxis-and-cell-length-in-azospirillum-brasilense-sp7
#20
Jessica M Gullett, Amber Bible, Gladys Alexandre
Chemotaxis is the movement of cells in response to gradients of diverse chemical cues. Motile bacteria utilize a conserved chemotaxis signal transduction system to bias their motility and navigate through a gradient. A central regulator of chemotaxis is the histidine kinase CheA. This cytoplasmic protein interacts with membrane-bound receptors, which assemble into large polar arrays, to propagate the signal. In the alphaproteobacterium Azospirillum brasilense, Che1 controls transient increases in swimming speed during chemotaxis, but it also biases the cell length at division...
July 1, 2017: Journal of Bacteriology
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