keyword
https://read.qxmd.com/read/38278051/pax3-mutation-suppress-otic-progenitors-proliferation-and-induce-apoptosis-by-inhibiting-wnt1-%C3%AE-catenin-signaling-pathway-in-ws1-patient-ipsc-derived-inner-ear-organoids
#21
JOURNAL ARTICLE
SiJun Li, Chufeng He, Lingyun Mei, Xuewen Wu, Yong Feng, Jian Song
Waardenburg syndrome type 1 (WS1) is a hereditary disease mainly characterized by sensorineural hearing loss, dystopia canthorum, and pigmentary defects. To elucidate molecular mechanisms underlying PAX3-associated hearing loss, we developed inner ear organoids model using induced pluripotent stem cells (iPSCs) derived from WS1 patient and healthy individual. Our results revealed a significant reduction in the size of inner ear organoids, accompanied by an increased level of apoptosis in organoids derived from WS1 patient-iPSCs carrying PAX3 c...
January 13, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38250801/sequencing-reveals-mirnas-enriched-in-the-developing-mouse-enteric-nervous-system
#22
JOURNAL ARTICLE
Christopher Pai, Rajarshi Sengupta, Robert O Heuckeroth
The enteric nervous system (ENS) is an essential network of neurons and glia in the bowel wall. Defects in ENS development can result in Hirschsprung disease (HSCR), a life-threatening condition characterized by severe constipation, abdominal distention, bilious vomiting, and failure to thrive. A growing body of literature connects HSCR to alterations in miRNA expression, but there are limited data on the normal miRNA landscape in the developing ENS. We sequenced small RNAs (smRNA-seq) and messenger RNAs (mRNA-seq) from ENS precursor cells of mid-gestation Ednrb-EGFP mice and compared them to aggregated RNA from all other cells in the developing bowel...
December 22, 2023: Non-Coding RNA
https://read.qxmd.com/read/38234039/pth1r-in-neural-crest-cells-regulates-nasal-cartilage-differentiation
#23
JOURNAL ARTICLE
K Amano, D Okuzaki, Y Kitaoka, S Kato, M Fujiwara, S Tanaka, S Iida
Neural crest cells (NCC) arise from the dorsal margin of the neural plate border and comprise a unique cell population that migrates to and creates the craniofacial region. Although factors including Shh, Fgf8, and bone morphogenetic proteins have been shown to regulate these biological events, the role of parathyroid hormone 1 receptor (Pth1r) has been less studied. We generated an NCC-specific mouse model for Pth1r and researched gene expression, function, and interaction focusing on nasal cartilage framework and midfacial development...
January 17, 2024: Journal of Dental Research
https://read.qxmd.com/read/38224919/functionality-of-bone-marrow-mesenchymal-stromal-cells-derived-from-head-and-neck-cancer-patients-a-fda-ind-enabling-study-regarding-msc-based-treatments-for-radiation-induced-xerostomia
#24
JOURNAL ARTICLE
Grace C Blitzer, Cristina Paz, Annemarie Glassey, Olga R Ganz, Jayeeta Giri, Andrea Pennati, Ross O Meyers, Amber M Bates, Kwangok P Nickel, Marissa Weiss, Zachary S Morris, Ryan J Mattison, Kimberly A McDowell, Emma Croxford, Richard J Chappell, Tiffany A Glazer, Nicole M Rogus-Pulia, Jacques Galipeau, Randall J Kimple
PURPOSE: Salivary dysfunction is a significant side effect of radiation therapy for head and neck cancer (HNC). Preliminary data suggests that mesenchymal stromal cells (MSCs) can improve salivary function. Whether MSCs from HNC patients who have completed chemoradiation are functionally similar to those from healthy patients is unknown. We performed a pilot clinical study to determine whether bone marrow-derived MSCs [MSC(M)] from HNC patients could be used for the treatment of RT-induced salivary dysfunction...
January 13, 2024: Radiotherapy and Oncology
https://read.qxmd.com/read/38182576/ionizing-radiation-induces-cells-with-past-caspase-activity-that-contribute-to-the-adult-organ-in-drosophila-and-show-reduced-loss-of-heterozygosity
#25
JOURNAL ARTICLE
Sarah Colon Plaza, Tin Tin Su
There is increasing recognition that cells may activate apoptotic caspases but not die, instead displaying various physiologically relevant consequences. Mechanisms that underlie the life-or-death decision in a cell that has activated apoptotic caspases, however, are incompletely understood. By optimizing a published reporter for past caspase activity, we were able to visualize cells that survived caspase activation specifically after exposure to ionizing radiation in Drosophila larval wing discs. We found that cells with X-ray-induced past active caspases (XPAC) did not arise at random but were born at specific locations within the developing wing imaginal discs of Drosophila larvae...
January 5, 2024: Cell Death Discovery
https://read.qxmd.com/read/38171557/-generation-of-skin-derived-ipscs-from-an-osteogenesis-imperfecta-patient-carrying-wnt1-c-677c-t-mutation
#26
JOURNAL ARTICLE
Songjie Du, Xin Guan, Meili Zhang, Xiuli Zhao, Yue Huang
OBJECTIVE: To obtain skin-derived induced pluripotent stem cells (iPSCs) from an Osteogenesis imperfecta (OI) patient carrying WNT1c.677C>T mutation in order to provide a new cell model for investigating the underlying molecular mechanism and stem cell therapy for OI. METHODS: The pathogenic variant of the patient was identified by Sanger sequencing. With informed consent from the patient, skin tissue was biopsied, and primary skin fibroblasts were cultured. Skin fibroblasts were induced into iPSCs using Sendai virus-mediated non-genomic integration reprogramming method...
January 10, 2024: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://read.qxmd.com/read/38167468/loss-of-g9a-does-not-phenocopy-the-requirement-for-prdm12-in-the-development-of-the-nociceptive-neuron-lineage
#27
JOURNAL ARTICLE
Panagiotis Tsimpos, Simon Desiderio, Pauline Cabochette, Philippe Poelvoorde, Sadia Kricha, Luc Vanhamme, Coralie Poulard, Eric J Bellefroid
Prdm12 is an epigenetic regulator expressed in developing and mature nociceptive neurons, playing a key role in their specification during neurogenesis and modulating pain sensation at adulthood. In vitro studies suggested that Prdm12 recruits the methyltransferase G9a through its zinc finger domains to regulate target gene expression, but how Prdm12 interacts with G9a and whether G9a plays a role in Prdm12's functional properties in sensory ganglia remain unknown. Here we report that Prdm12-G9a interaction is likely direct and that it involves the SET domain of G9a...
January 2, 2024: Neural Development
https://read.qxmd.com/read/38158162/investigation-of-iso-propylchaetominine-anticancer-activity-on-apoptosis-cell-cycle-and-wnt-signaling-pathway-in-different-cancer-models
#28
JOURNAL ARTICLE
Vahidreza Karamad, Fatma Sogutlu, Ferhat Can Ozkaya, Behrouz Shademan, Weaam Ebrahim, Mona El-Neketi, Cigir Biray Avci
Dysregulation of the Wnt signaling pathway contributes to the development of many cancer types. Natural compounds produced with biotechnological systems have been the focus of research for being a new drug candidate both with unlimited resources and cost-effective production. In this study, it was aimed to reveal the effects of isopropylchaetominine on cytotoxic, cytostatic, apoptotic and Wnt signaling pathways in brain, pancreatic and prostate cancer. The IC50 values of isopropylchaetominine in U-87 MG, PANC1, PC3 and LNCaP cells were calculated as 91...
December 27, 2023: Fitoterapia
https://read.qxmd.com/read/38152126/wnt-%C3%AE-catenin-signaling-activation-promotes-lipogenesis-in-the-steatotic-liver-via-physical-mtor-interaction
#29
JOURNAL ARTICLE
Kewei Wang, Rong Zhang, Nadja Lehwald, Guo-Zhong Tao, Bowen Liu, Bo Liu, Yangseok Koh, Karl G Sylvester
BACKGROUND AND AIMS: Wnt/β-catenin signaling plays an important role in regulating hepatic metabolism. This study is to explore the molecular mechanisms underlying the potential crosstalk between Wnt/β-catenin and mTOR signaling in hepatic steatosis. METHODS: Transgenic mice (overexpress Wnt1 in hepatocytes, Wnt+) mice and wild-type littermates were given high fat diet (HFD) for 12 weeks to induce hepatic steatosis. Mouse hepatocytes cells (AML12) and those transfected to cause constitutive β-catenin stabilization (S33Y) were treated with oleic acid for lipid accumulation...
2023: Frontiers in Endocrinology
https://read.qxmd.com/read/38145300/wisp1-induces-the-expression-of-macrophage-migration-inhibitory-factor-mif-in-human-lung-fibroblasts-through-src-kinases-and-egfr-activated-signaling-pathways
#30
JOURNAL ARTICLE
Maria-Elpida Christopoulou, Spyros S Skandalis, Eleni Papakonstantinou, Daiana Stolz, Alexios J Aletras
Wnt1-inducible signaling protein 1 (WISP1/CCN4) is a secreted matricellular protein that is implicated in lung and airway remodeling. The macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine that has been associated with chronic lung diseases. In this study, we aimed to investigate the WISP1 signalling pathway and its ability to induce the expression of MIF in primary cultures of fibroblasts from normal human lung (HLFs). Our results showed that WISP1 significantly stimulated the expression of MIF in a concentration- and time-dependent fashion...
December 25, 2023: American Journal of Physiology. Cell Physiology
https://read.qxmd.com/read/38139440/mirna-21a-mirna-145-and-mirna-221-expression-and-their-correlations-with-wnt-proteins-in-patients-with-obstructive-and-non-obstructive-coronary-artery-disease
#31
JOURNAL ARTICLE
Alfiya Oskarovna Iusupova, Nikolay Nikolaevich Pakhtusov, Olga Alexandrovna Slepova, Yuri Nikitich Belenkov, Elena Vitalievna Privalova, Irina Vladimirovna Bure, Ekaterina Alexandrovna Vetchinkina, Marina Vyacheslavovna Nemtsova
UNLABELLED: MicroRNAs and the WNT signaling cascade regulate the pathogenetic mechanisms of atherosclerotic coronary artery disease (CAD) development. OBJECTIVE: To evaluate the expression of microRNAs (miR-21a, miR-145, and miR-221) and the role of the WNT signaling cascade (WNT1, WNT3a, WNT4, and WNT5a) in obstructive CAD and ischemia with no obstructive coronary arteries (INOCA). METHOD: The cross-sectional observational study comprised 94 subjects...
December 18, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/38139379/off-target-effects-of-p2y12-receptor-inhibitors-focus-on-early-myocardial-fibrosis-modulation
#32
REVIEW
Francesca Lofrumento, Natasha Irrera, Roberto Licordari, Silvia Perfetti, Enrica Nasso, Paolo Liotta, Giovanni Isgrò, Victoria Garcia-Ruiz, Francesco Squadrito, Scipione Carerj, Gianluca Di Bella, Antonio Micari, Francesco Costa
Several studies have demonstrated that, beyond their antithrombotic effects, P2Y12 receptor inhibitors may provide additional off-target effects through different mechanisms. These effects range from the preservation of endothelial barrier function to the modulation of inflammation or stabilization of atherosclerotic plaques, with an impact on different cell types, including endothelial and immune cells. Many P2Y12 inhibitors have been developed, from ticlopidine, the first thienopyridine, to the more potent non-thienopyridine derivatives such as ticagrelor which may promote cardioprotective effects following myocardial infarction (MI) by inhibiting adenosine reuptake through sodium-independent equilibrative nucleoside transporter 1 (ENT1)...
December 16, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/38114153/-blaps-rynchopetera-combined-with-cyclophosphamide-affects-proliferation-and-apoptosis-of-lung-cancer-cells-via-wnt-%C3%AE-catenin-signaling-pathway
#33
JOURNAL ARTICLE
Jing-Nan Yan, Ke Ma, Wen-Jie Liu, Ying Lin, Xiu-Yu Li, Dan Wu
This study aims to investigate the effects of Blaps rynchopetera Fairmaire and/or cyclophosphamide on the proliferation and apoptosis of lung cancer cells and decipher the underlying mechanism. B. rynchopetera and cyclophosphamide-containing serum and blank serum were prepared from SD rats. Cell counting kit-8(CCK-8) assay was employed to examine the proliferation of lung cancer cell lines A549 and Lewis treated with corresponding agents. The Jin's formula method was used to evaluate the combined effect of the two drugs...
October 2023: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://read.qxmd.com/read/38105830/evaluation-of-expression-of-wnt1-and-ptch-genes-in-peripheral-blood-of-patients-with-odontogenic-cysts-and-tumours-of-the-jaws-by-quantitative-rt-pcr-a-pilot-study
#34
JOURNAL ARTICLE
Srinath Kalyanasundaram, Saravanan Kandasamy, Reena Rachel John
INTRODUCTION: Odontogenic lesions of the maxillofacial region constitute a complex group of lesions with diverse histopathologic types and clinical behaviour. Early diagnosis is important to minimize the need for radical surgery and to improve quality of life of the patients. Tumour markers play an essential role in the molecular level understanding of Odontogenic lesions and also used for early diagnosis and target therapies which improves the quality of life of the patients. Patched, a tumour suppressor gene encodes the transmembrane protein PTCH and is a receptor for the morphogen Sonic Hedgehog...
December 2023: Journal of Maxillofacial and Oral Surgery
https://read.qxmd.com/read/38099414/inhibitor-of-wnt-receptor-1-suppresses-the-effects-of-wnt1-wnt3a-and-%C3%AE-%C3%A2-catenin-on-the-proliferation-and-migration-of-c6-gscs-induced-by-low%C3%A2-dose-radiation
#35
JOURNAL ARTICLE
Yu Yan, Ying-Ying Cheng, Yan-Ru Li, Xu-Wen Jiao, Yin-Ming Liu, Hai-Yan Cai, Yin-Xiu Ding
The radioresistance of glioma is an important cause of treatment failure and tumor aggressiveness. In the present study, under performed with linear accelerator, the effects of 0.3 and 3.0 Gy low‑dose radiation (LDR) on the proliferation and migration of C6 glioma stem cells in vitro were examined by flow cytometric analysis, immunocytochemistry and western blot analysis. It was found that low‑dose ionizing radiation (0.3 Gy) stimulated the proliferation and migration of these cells, while 3...
February 2024: Oncology Reports
https://read.qxmd.com/read/38079449/autonomous-and-non-cell-autonomous-role-of-cilia-in-structural-birth-defects-in-mice
#36
JOURNAL ARTICLE
Richard J B Francis, Jovenal T San Agustin, Heather L Szabo Rogers, Cheng Cui, Julie A Jonassen, Thibaut Eguether, John A Follit, Cecilia W Lo, Gregory J Pazour
Ciliopathies are associated with wide spectrum of structural birth defects (SBDs), indicating important roles for cilia in development. Here, we provide novel insights into the temporospatial requirement for cilia in SBDs arising from deficiency in Ift140, an intraflagellar transport (IFT) protein regulating ciliogenesis. Ift140-deficient mice exhibit cilia defects accompanied by wide spectrum of SBDs including macrostomia (craniofacial defects), exencephaly, body wall defects, tracheoesophageal fistula (TEF), randomized heart looping, congenital heart defects (CHDs), lung hypoplasia, renal anomalies, and polydactyly...
December 11, 2023: PLoS Biology
https://read.qxmd.com/read/38072026/inulin-alleviates-perfluorooctanoic-acid-induced-intestinal-injury-in-mice-by-modulating-the-pi3k-akt-mtor-signaling-pathway
#37
JOURNAL ARTICLE
Qin-Yao Zhang, Mei-Ting Zhong, Min Gi, Yu-Kui Chen, Ming-Quan Lai, Jing-Yi Liu, Yi-Ming Liu, Qi Wang, Xiao-Li Xie
Perfluorooctanoic acid (PFOA) is a widely used industrial compound that has been found to induce intestinal toxicity. However, the underlying mechanisms have not been fully clarified and effective interventions are rarely developed. Inulin, a prebiotic, has been used as a supplement in human daily life as well as in gastrointestinal diseases and metabolic disorders. In this study, male mice were exposed to PFOA with or without inulin supplementation to investigate the enterotoxicity and potential intervention effects of inulin...
December 8, 2023: Environmental Pollution
https://read.qxmd.com/read/38053912/study-of-genes-polymorphisms-in-rank-rankl-opg-and-wnt-signaling-pathways-and-their-associations-with-bone-parameters-in-broiler-chicken
#38
JOURNAL ARTICLE
Michala Steinerova, Cenek Horecky, Ales Knoll, Sarka Nedomova, Petr Slama, Ales Pavlik
Limb problems are one of the most common problems with fast-growing meat-type chickens. Various bone abnormalities, which can lead to limping, bone weakness, or even fractures, bring overall discomfort to birds and a loss of production. Genetic aspects are often associated with these side effects on bone stability and are also cited as the dominant cause. These points to a close negative relationship of genetic selection for rapid growth with traits involved in bone integrity. Due to the assumption of an additive genetic background, improvements through genetic tools can be used...
November 2023: Heliyon
https://read.qxmd.com/read/38035707/snhg3-wisp2-axis-promotes-hela-cell-migration-and-invasion-via-activating-wnt-%C3%AE-catenin-signaling
#39
JOURNAL ARTICLE
Dengfei Xu, Hao Feng, Zirui Ren, Xiang Li, Chenyang Jiang, Yuming Chen, Lina Liu, Wenchao Chen, Zhilei Cui, Shundong Cang
BACKGROUND/AIM: Cervical cancer (CC) poses a significant threat to women's health and has a relatively poor prognosis due to local invasion and metastasis. It is, therefore, crucial to elucidate the molecular mechanisms of CC metastasis. SNHG3 has been implicated in various tumor metastasis processes, but its involvement in CC has not been thoroughly studied. Our study aimed to investigate the role of SNHG3 in metastasis and elucidate its underlying mechanisms in CC. MATERIALS AND METHODS: LncRNA SNHG3 expression in CC tissues was analyzed using TCGA and GSE27469 databases...
December 2023: Cancer Genomics & Proteomics
https://read.qxmd.com/read/38022638/the-impact-of-aging-and-oxidative-stress-in-metabolic-and-nervous-system-disorders-programmed-cell-death-and-molecular-signal-transduction-crosstalk
#40
REVIEW
Kenneth Maiese
Life expectancy is increasing throughout the world and coincides with a rise in non-communicable diseases (NCDs), especially for metabolic disease that includes diabetes mellitus (DM) and neurodegenerative disorders. The debilitating effects of metabolic disorders influence the entire body and significantly affect the nervous system impacting greater than one billion people with disability in the peripheral nervous system as well as with cognitive loss, now the seventh leading cause of death worldwide. Metabolic disorders, such as DM, and neurologic disease remain a significant challenge for the treatment and care of individuals since present therapies may limit symptoms but do not halt overall disease progression...
2023: Frontiers in Immunology
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