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https://www.readbyqxmd.com/read/28109198/mir-143-induces-the-apoptosis-of-prostate-cancer-lncap-cells-by-suppressing-bcl-2-expression
#1
Zhiwei Ma, Yizhao Luo, Mingxing Qiu
BACKGROUND Prostate cancer has become a serious threat to the life of patients. microRNAs are small non-coding RNA molecules that regulate the growth and apoptosis of cells. We aimed to investigate the regulation and mechanism of microRNA (miR-143) in the proliferation and apoptosis of prostate cancer LNCap cells. MATERIAL AND METHODS miR-143 and control scramble miRNA were synthesized and respectively transfected into LNCap cells. The proliferation and apoptosis were detected by MTT assay, flow cytometry, and caspase-3 activity assay...
January 21, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28109197/everolimus-is-better-than-rapamycin-in-attenuating-neuroinflammation-in-kainic-acid-induced-seizures
#2
Ming-Tao Yang, Yi-Chin Lin, Whae-Hong Ho, Chao-Lin Liu, Wang-Tso Lee
BACKGROUND: Microglia is responsible for neuroinflammation, which may aggravate brain injury in diseases like epilepsy. Mammalian target of rapamycin (mTOR) kinase is related to microglial activation with subsequent neuroinflammation. In the present study, rapamycin and everolimus, both as mTOR inhibitors, were investigated in models of kainic acid (KA)-induced seizure and lipopolysaccharide (LPS)-induced neuroinflammation. METHODS: In vitro, we treated BV2 cells with KA and LPS...
January 21, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28109186/interleukin-1-as-a-mediator-of-fatigue-in-disease-a-narrative-review
#3
REVIEW
Megan E Roerink, Marieke E van der Schaaf, Charles A Dinarello, Hans Knoop, Jos W M van der Meer
Fatigue is commonly reported in a variety of illnesses, and it has major impact on quality of life. Previously, it was thought that fatigue originates in the skeletal muscles, leading to cessation of activity. However, more recently, it has become clear that the brain is the central regulator of fatigue perception. It has been suggested that pro-inflammatory cytokines, especially interleukin-1 alpha (IL-1α) and interleukin-1 beta (IL-1β), play a prominent role in the development of central fatigue, and several studies have been performed to elucidate the connection between inflammation and these central processes...
January 21, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28109183/chloride-ion-transport-and-overexpression-of-tmem16a-in-a-guinea-pig-asthma-model
#4
Mitsuko Kondo, Mayoko Tsuji, Kaori Hara, Ken Arimura, Osamitsu Yagi, Etsuko Tagaya, Kiyoshi Takeyama, Jun Tamaoki
BACKGROUND: TMEM16A, a Ca-activated Cl channel, regulates various physiological functions such as mucin secretion. However, the role of TMEM16A in hypersecretion in asthma is not fully understood. OBJECTIVE: The aim of this study is to evaluate Cl ion transport via TMEM16A and determine the localization of TMEM16A in a guinea pig asthma model. METHODS: Guinea pigs were sensitized with OVA ip. on Day 1 and Day 8. On Day 22, we assessed OVA challenge-induced Cl ion transport in the sensitized tracheas ex vivo in an Ussing chamber, compared with the non-sensitized tracheas...
January 21, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28109180/the-intrauterine-renin-angiotensin-system-sex-specific-effects-on-the-prevalence-of-spontaneous-preterm-birth
#5
Kirsty G Pringle, Tamas Zakar, Eugenie R Lumbers
Preterm birth (PTB) is the single largest cause of death in infants and young children. The rate of PTB is significantly higher in male infants, particularly those that are born very preterm. Here we present evidence to suggest that the decidual renin-angiotensin system may play a role in inhibiting inflammation and maintaining the integrity of the fetal membranes during pregnancy, and that sex-specific alterations in the intrauterine RAS could contribute to the increased risk of PTB in male babies. Women carrying female fetuses have high levels of expression of decidual prorenin at term...
January 21, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28109177/glutamine-synthetase-stabilizes-the-binding-of-glnr-to-nitrogen-fixation-gene-operators
#6
Gabriela de Carvalho Fernandes, Ksenia Hauf, Fernando Hayashi Sant'Anna, Karl Forchhammer, Luciane Maria Pereira Passaglia
Biological nitrogen fixation (BNF) is a high energy demanding process carried out by diazotrophic microorganisms that supply combined nitrogen to the biosphere. The genes related to BNF are strictly regulated, but these mechanisms are poorly understood in Gram-positive bacteria. The transcription factor GlnR was proposed to regulate nitrogen fixation related genes based on Paenibacillus comparative genomics. In order to validate this proposal, we investigated BNF regulatory sequences in Paenibacillus riograndensis SBR5(T) genome...
January 21, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28109154/-inhibitory-effect-of-overexpressed-kisspeptin-on-proliferation-and-migration-of-mkn-45-gastric-cancer-cells
#7
Xin Wang, Yijiong Li, Ruihua Zhang, Chao Pang, Xiaoqing Jiao, Zhenlong Zhu, Zhengmin Wang
Objective To investigate the expression of kisspeptin in gastric adenocarcinoma and its effect on the proliferation and migration of gastric cancer cells. Methods The level of kisspeptin in 50 gastric cancer tissues and their paracancerous tissues were detected by quantitative real-time PCR and Western blotting. Kisspeptin-siRNA, control-siRNA, pEGFP-N1-kisspeptin, pEGFP-N1 were separately transfected into MKN-45 gastric cancer cells. After 48-hour culture, the levels of matrix metalloproteinase-2 (MMP-2), β-catenin, C-myc, MMP-9, kisspeptin were detected by Western blotting; MTT assay was used to detect the proliferation of MKN-45 cells; wound healing assay was performed to assess the migration ability of MKN-45 cells...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109152/-hepatic-stellate-cell-conditioned-medium-induces-proliferation-and-epithelial-mesenchymal-transition-via-activating-erk1-2-signaling-pathway-in-hepatoma-cells
#8
Yuxiao Xie, Rui Liao, Long Pan, Kai Fan, Cong Peng, Chengyou Du
Objective To investigate the influence of hepatic stellate cells (HSCs) on malignant biological behavior of hepatoma cells and related mechanisms. Methods Human hepatoma cell lines SMMC-7721 and HepG2, and hepatic stellate cell line LX-2 were cultured separately. HSC conditioned medium (LX2-CM), MAPK specific inhibitor U0126 were used to treat hepatoma cells, separately or together. The invasion and migration abilities of hepatoma cells were detected by Transwell(TM) assay, and cell proliferation was analyzed by CCK-8 assay...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109148/-rat-bone-marrow-mesenchymal-stem-cells-promote-apoptosis-and-inhibit-proliferation-and-migration-of-rsc96-cells
#9
Yongbin Ma, Suohua Ge, Dan Zhou, Huanyan Zhang, Luyang Sun, Xuefeng Wang, Jianhua Su
Objective To investigate the effect of rat bone marrow mesenchymal stem cells (BMSCs) on the proliferation and migration of rat RSC96 Schwann cells in vitro. Methods BMSCs of Sprague Dawley (SD) rats were isolated by bone marrow cell adherent method. The morphology of the third generation of BMSCs was observed by inverted microscopy. The cell surface antigen makers CD19, CD34, CD73, CD105 were detected by flow cytometry. The third-passage BMSCs differentiated into osteoblasts and adipocytes after exposed to bone-inducing and lipid-inducing media for 3 and 2 weeks, respectively, and then were identified by oil red O and alkaline phosphatase staining...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109147/-co-culture-with-umbilical-cord-mesenchymal-stem-cells-promotes-the-synthesis-and-mechnism-of-milk-protein-in-bovine-mammary-epithelial-cells
#10
Yankun Zhao, Wei Shao, Chenglong Luo, Kaile Wu, Xiong Yu
Objective To explore the possible mechanism of umbilical cord mesenchymal stem cells (UCMSCs) regulating milk protein synthesis in bovine mammary gland epithelial cells (BMECs). Methods UCMSCs and BMECs were co-cultured by double-chamber Transwell(TM), and other BMECs were cultured alone as a control group. Insulin like growth factor 1 receptor (IGF-1R) inhibitor AG1024 was used to treat cells. IGF-1, β casein (CSN2) and κ casein (CSN3) content in the supernatants were determined by ELISA; the relative expression abundance of Janus kinase and signal transducer and activator of transcription factor (JAK/STAT) signaling pathway-related genes were detected by quantitative real-time PCR (qRT-PCR); and after the cells were treated with JAK2 signal blocker AG490, qRT-PCR was performed to test the relative expression abundance of CSN2 and CSN3 mRNAs...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109145/-short-term-use-of-rapamycin-combined-with-tregs-prolongs-mouse-cardiac-graft-survival-but-attenuates-recipient-s-anti-tumor-immunity
#11
Ting Yan, Chen Luo, Xianmo Yang, Lei Ji, Shiqiao Luo
Objective To study the effect of short-term use of rapamycin(Rap) combined with regulatory T cells (Tregs) on the long-time survival of allogeneic mouse cardiac transplant, and its impact on the anti-tumor immunity of recipient. MethodsMouse Tregs were purified from recipients' spleen by magnetic activated cell sorting (MACS), and expanded by CD3/CD28 monoclonal antibody immunomagnetic beads and 2000 U/mL recombinant mouse IL-2 (rmIL-2) ex vivo. The purity was tested by fluorescence-activated cell sorting (FACS)...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109141/-hydrogen-rich-saline-attenuates-hyperalgesia-and-reduces-cytokines-in-rats-with-post-herpetic-neuralgia-via-activating-autophagy
#12
Hongtao Ma, Hongguang Chen, Aili Dong, Yanyan Wang, Yingxue Bian, Keliang Xie
Objective To investigate the role of autophagy in hydrogen-rich saline attenuating post-herpetic neuralgia (PHN) in rats. Methods A total of 100 male SD rats were randomly divided into the five groups (n=20): control group, PHN group, PHN group treated with hydrogen-rich saline (PHN-H2 group), PHN group treated with hydrogen-rich saline and 3-MA (PHN-H2-3-MA group), PHN group treated with hydrogen-rich saline and rapamycin (PHN-H2-Rap group). PHN models were established by varicella-zoster virus (VZV) inoculation...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109130/identification-of-promising-dna-gyrb-inhibitors-for-tuberculosis-using-pharmacophore-based-virtual-screening-molecular-docking-and-molecular-dynamics-studies
#13
Md Ataul Islam, Tahir S Pillay
In the current study, we searched for potential DNA GyrB inhibitors using pharmacophore-based virtual screening followed by molecular docking and molecular dynamics simulation approaches. For this purpose, a set of 248 DNA GyrB inhibitors were collected from the literature and a well-validated pharmacophore model was generated. The best pharmacophore model explained that two each of hydrogen bond acceptor and hydrophobicity were critical for inhibition of DNA GyrB. Good statistical results of the pharmacophore model indicated that the model was robust in nature...
January 21, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28109129/vsl-3-probiotic-differently-influence-iec-6-intestinal-epithelial-cell-status-and-function
#14
REVIEW
Benedetta Cinque, Cristina La Torre, Francesca Lombardi, Paola Palumbo, Zoran Evtoski, Silvano Jr Santini, Stefano Falone, Annamaria Cimini, Fernanda Amicarelli, Maria Grazia Cifone
The data here reported introduce the wound-healing assay as a tool for testing probiotics aimed at protecting gastrointestinal mucosal surfaces and to verify the consistency of their manufacturing. At the scope, we compared the in vitro effects of two multi-strain high concentration formulations both commercialized under the same brand VSL#3 but sourced from different production sites (USA and Italy) on a non-transformed small-intestinal epithelial cell line, IEC-6. The effects on cellular morphology, viability, migration, and H2 O2 -induced damage, were assessed before and after the treatment with both VSL#3 formulations...
January 21, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28109123/sodium-butyrate-protects-against-high-fat-diet-induced-cardiac-dysfunction-and-metabolic-disorders-in-type-ii-diabetic-mice
#15
Ling Zhang, Jianfeng Du, Naohiro Yano, Hao Wang, Yu Tina Zhao, Dubielecka-Szczerba Patricia, Shougang Zhuang, Eugene Y Chin, Gangjian Qin, Ting C Zhao
Histone deacetylases are recently identified to act as key regulators for cardiac pathophysiology and metabolic disorders. However, the function of histone deacetylase (HDAC) in controlling cardiac performance in type II diabetes and obesity remains unknown. Here we determine whether HDAC inhibition attenuates high fat diet (HFD)-induced cardiac dysfunction and improves metabolic features. Adult mice were fed with either HFD or standard chow food for 24 weeks. Starting at 12 weeks, mice were divided into four groups randomly, in which sodium butyrate (1%), a potent HDAC inhibitor, was provided to chow and HFD-fed mice in drinking water, respectively...
January 21, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28109113/-effect-of-sodium-phenylbutyrate-on-the-sensitivity-of-pc3-dtx-resistant-prostate-cancer-cells-to-docetaxel
#16
Ya-Wen Xu, Shao-Bo Zheng, Bin-Sheng Chen, Yong Wen, Shan-Wen Zhu
OBJECTIVE: To investigate the effect of sodium phenylbutyrate (SPB) in modulating docetaxel resistance in human prostate cancer cells in vitro. METHODS: A PC3/docetaxel-resistant human prostate cancer cell line PC3/DTX was induced and examined for proliferation, viability, and cell inhibition rate in the presence of SPB. The concentration of concentration of docetaxel required to kill 50% of PC3/DTX cells incubated with 0, 1, 2, and 4 mmol/L SPB was determined using MTT assay...
2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28109106/-effect-of-methamphetamine-exposure-on-s-nitrosylation-of-protein-disulphide-isomerase-in-pc12-cells
#17
Xiao-Fang Wu, Ai-Feng Wang, Ping-Ming Qiu
OBJECTIVE: To study the effect of methamphetamine (METH) exposure on S-nitrosylation of protein disulphide isomerase and the neurotoxicity of METH in PC12 cells. METHODS: PC12 cells were exposed to different concentrations of METH, and the cell viability was assessed using the cell-counting kit-8. PC12 cells exposed to METH in the presence of the NOS inhibitor N-nitro-L-arginine (L-NNA) were examined for cell viability and S-nitrosylation of protein disulphide isomerase using the biotin-switch method, and the changes in cell morphology were examined with HE staining...
January 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28109094/-lactobacillus-rhamnosus-gg-conditioned-medium-prevents-e-coli-meningitis-by-inhibiting-nuclear-factor-%C3%AE%C2%BAb-pathway
#18
Qing Zeng, Xiao-Long He, Han-Sheng Xiao, Lei DU, Yu-Jing Li, Le-Cheng Chen, Hui-Wen Tian, Sheng-He Huang, Hong Cao
OBJECTIVE: To investigate whether Lactobacillus rhamnosus GG conditioned medium(LGG-CM)has preventive effect against E. coli K1-induced neuropathogenicity in vitro by inhibiting nuclear factor-κB (NF-κB) signaling pathway. METHODS: An in vitro blood-brain barrier (BBB) model was constructed using human brain microvascular endothelial cells (HBMECs). The effect of LGG-CM on E. coli-actived NF-κB signaling pathway was assayed using Western blotting. Invasion assay and polymorphonuclear leukocyte (PMN) transmigration assay were performed to explore whether LGG-CM could inhibit E...
January 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28109089/betulinic-acid-inhibits-cell-proliferation-in-human-oral-squamous-cell-carcinoma-via-modulating-ros-regulated-p53-signaling
#19
Huan Shen, Li Liu, Yongjin Yang, Wenxing Xun, Kewen Wei, Guang Zeng
Oral squamous cell carcinoma (OSCC) is a common cancer of the head and neck. Betulinic acid (BA) is a naturally occurring pentacyclic triterpenoid. The present study was designed to explore the effects of BA on OSCC KB cell proliferation <em>in vitro</em> and implanted tumor growth <em>in vivo</em> and to examine the possible molecular mechanisms. The results showed that BA dose-dependently inhibited KB cell proliferation and decreased implanted tumor volume. In addition, BA significantly promoted mitochondrial apoptosis, as reflected by increase of TUNEL-positive cells and activities of caspase 3 and 9, increase of Bax expression and decrease of Bcl-2 expression and mitochondrial oxygen consumption rate...
January 20, 2017: Oncology Research
https://www.readbyqxmd.com/read/28109086/grade-dependent-expression-of-nima-related-kinase-2-in-glioma-and-its-cellular-upregulatory-activity-on-proliferation-and-invasion
#20
Li Tian, Peng Cheng, Huaiyu Sun, Yulin Wang, Lei Zhang, Zongze Guo
Glioma is one of the most common tumors in central nervous system. The median life expectancy for glioma patients is only 13-15 months. NIMA-related expressed kinase 2 (NEK2) is a kind of serine-threonine kinases and involves in carcinogenesis and progression of cancer, however, the specific roles in the glioma remains unknown. We found the high expression of NEK2 was positively correlated with deteriorated severity of glioma. Therefore, we sought to investigate the effects of NEK2 on U87 and U251 cells. To explore the expression of NEK2 and alteration of signaling pathway factors, qRT-PCR and Western blot analysis were performed...
January 20, 2017: Oncology Research
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