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spermatogonial stem cell

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https://www.readbyqxmd.com/read/29326073/retinoic-acid-promotes-stem-cell-differentiation-and-embryonic-development-by-transcriptionally-activating-cftr
#1
Xiaofeng Li, Kin Lam Fok, Jinghui Guo, Yan Wang, Zhenqing Liu, Ziyi Chen, Chengdong Wang, Ye Chun Ruan, Sidney Siubun Yu, Hui Zhao, Ji Wu, Xiaohua Jiang, Hsiao Chang Chan
Retinoic acid (RA) plays a pivotal role in many cellular processes; however, the signaling mechanisms mediating the effect of RA are not fully understood. Here, we show that RA transcriptionally upregulates cystic fibrosis transmembrane conductance regulator (Cftr) by promoting the direct binding of its receptor RARα to Cftr promoter in mouse spermatogonia and embryonic stem (ES) cells. The RA/CFTR pathway is involved in the differentiation of spermatogonia and organogenesis during the embryo development of Xenopus laevis...
January 8, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29305586/efficient-generation-of-functional-haploid-spermatids-from-human-germline-stem-cells-by-three-dimensional-induced-system
#2
Min Sun, Qingqing Yuan, Minghui Niu, Hong Wang, Liping Wen, Chencheng Yao, Jingmei Hou, Zheng Chen, Hongyong Fu, Fan Zhou, Chong Li, Shaorong Gao, Wei-Qiang Gao, Zheng Li, Zuping He
Generation of functional spermatids from human spermatogonial stem cells (SSCs) in vitro is of utmost importance for uncovering mechanisms underlying human germ cell development and treating infertility. Here we report a three-dimensional-induced (3D-I) system by which human SSCs were efficiently differentiated into functional haploid spermatids. Human SSCs were isolated and identified phenotypically. Meiotic chromatin spreads and DNA content assays revealed that spermatocytes and haploid cells were effectively generated from human SSCs by 3D-I system...
January 5, 2018: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29299873/single-cell-expression-profiling-and-proteomics-of-primordial-germ-cells-spermatogonial-stem-cells-adult-germ-stem-cells-and-oocytes
#3
Sabine Conrad, Hossein Azizi, Thomas Skutella
The mammalian germ cells, cell assemblies, tissues, and organs during development and maturation have been extensively studied at the tissue level. However, to investigate and understand the fundamental insights at the molecular basis of germ and stem cells, their cell fate plasticity, and determination, it is of most importance to analyze at the large scale on the single-cell level through different biological windows. Here, modern molecular techniques optimized for single-cell analysis, including single fluorescence-activated cell sorting (FACS) and single-cell RNA sequencing (scRNA-seq) or microfluidic high-throughput quantitative real-time polymerase chain reaction (qRT-PCR) for single-cell gene expression and liquid chromatography coupled to tandem mass spectrometry (LC-MSMS) for protein profiling, have been established and are still getting optimized...
January 4, 2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29299171/melatonin-up-regulates-the-expression-of-the-gata-4-transcription-factor-and-increases-testosterone-secretion-from-leydig-cells-through-ror%C3%AE-signaling-in-an-in-vitro-goat-spermatogonial-stem-cell-differentiation-culture-system
#4
Shou-Long Deng, Yan Zhang, Kun Yu, Xiu-Xia Wang, Su-Ren Chen, De-Ping Han, C Yan Cheng, Zheng-Xing Lian, Yi-Xun Liu
Because androgen function is regulated by its receptors, androgen-androgen receptor signaling is crucial for regulating spermatogenesis. Androgen is mainly testosterone secreted by testis. Based on the results of early studies in goats, the administration of melatonin over an extended period of time increases steroid production, but the underlying mechanism remains unclear. Here, we report the expression of the melatonin membrane receptors MT1 and MT2 and the retinoic acid receptor-related orphan receptor-alpha (RORα) in the goat testis...
December 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/29289671/melatonin-protects-mouse-spermatogonial-stem-cells-against-hexavalent-chromium-induced-apoptosis-and-epigenetic-histone-modification
#5
Yinghua Lv, Pengfei Zhang, Jiayin Guo, Zhendong Zhu, Xueliang Li, Dazhong Xu, Wenxian Zeng
Given the potential biological functions of spermatogonial stem cells (SSCs) in spermatogenesis and in delivering parental genetic information to the next generation, how these cells respond to environmental toxins and carcinogens should be investigated. We examined the toxic effect of hexavalent chromium (Cr(VI)) on global histone modifications and apoptotic signaling pathways in SSCs. We determined the effect of melatonin, one of the most powerful endogenous free radical scavengers and wide-spectrum antioxidants, in protecting SSCs from Cr(VI)-induced apoptosis and global histone modification by Western blot analysis...
December 28, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29281008/testicular-organoids-a-new-model-to-study-the-testicular-microenvironment-in-vitro
#6
João Pedro Alves-Lopes, Jan-Bernd Stukenborg
BACKGROUND: In recent decades, a broad range of strategies have been applied to model the testicular microenvironment in vitro. These models have been utilized to study testicular physiology and development. However, a system that allows investigations into testicular organogenesis and its impact in the spermatogonial stem-cell (SSC) niche in vitro has not been developed yet. Recently, the creation of tissue-specific organ-like structures called organoids has resurged, helping researchers to answer scientific questions that previous in vitro models could not help to elucidate...
December 21, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/29250763/transplantation-of-spermatogonial-stem-cells-suspension-into-rete-testis-of-azoospermia-mouse-model
#7
Arefeh Jafarian, Niknam Lakpour, Mohammad Reza Sadeghi, Sheida Salehkhou, Mohammad Mehdi Akhondi
PURPOSE: The loss of spermatogonia following chemo-or radiotherapy leading to temporary or permanent infertility of the patient is a well known and unwanted side effect of many oncological therapies. MATERIALS AND METHODS: In this study, germ cells were isolated from 4 days old mouse testis cells. Busulfan treatment was used to the eliminate proliferating cells in the testis of recipient mice. The donor cells suspended in DMEM, were introduced into the rete testis of recipient mice via microinjection method...
December 17, 2017: Urology Journal
https://www.readbyqxmd.com/read/29247571/testis-specific-lypd9-is-dispensable-for-spermatogenesis-in-mouse
#8
Junyan Feng, Puping Liang, Yuxi Chen, Xiya Zhang, Zhou Songyang, Haiyan Zheng, Shanbo Cao, Junjiu Huang
Lypd9 is a member of the Ly6/uPAR family. While Lypd9, display testis-specific gene expression in mouse, its function remains obscure. We proved that Lypd9 is a secreted protein specifically expressed in mouse testis. To investigate the function of Lypd9 in male mouse reproduction, we generated Lypd9 knockout mice using the CRISPR/Cas9 system. Body weight and testis development of Lypd9 null mice were comparable to wildtype mice. We found that disruption of Lypd9 did not affect the number of spermatogonial stem cells, meiosis progression or XY body formation, as was determined by immunofluorescence staining with PLZF, SCP3 and î3 H2AX...
December 16, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29241545/the-germline-linker-histone-dbigh1-and-the-translational-regulator-bam-form-a-repressor-loop-essential-for-male-germ-stem-cell-differentiation
#9
Albert Carbonell, Salvador Pérez-Montero, Paula Climent-Cantó, Oscar Reina, Fernando Azorín
Drosophila spermatogenesis constitutes a paradigmatic system to study maintenance, proliferation, and differentiation of adult stem cell lineages. Each Drosophila testis contains 6-12 germ stem cells (GSCs) that divide asymmetrically to produce gonialblast cells that undergo four transit-amplifying (TA) spermatogonial divisions before entering spermatocyte differentiation. Mechanisms governing these crucial transitions are not fully understood. Here, we report the essential role of the germline linker histone dBigH1 during early spermatogenesis...
December 12, 2017: Cell Reports
https://www.readbyqxmd.com/read/29221621/-fertility-preservation-in-oncology
#10
REVIEW
Laure Chaput, Anne-Sophie Grémeau, Solène Vorilhon, Hanae Pons, Cécile Chabrot, Victoria Grèze, Jean-Luc Pouly, Florence Brugnon
Since the improvement of cancer diagnosis and treatment, survival rates of these patients increase. Gonadal damages are frequent consequences of cancer treatments with different evidence of impaired fertility. In this context, fertility preservation should be proposed to patients exposed to potentially gonadotoxic treatments. Different preservation approaches may be proposed depending on patient age, sex, cancer type and type of treatment. The indications of fertility preservation depend on sexual maturity...
December 5, 2017: Bulletin du Cancer
https://www.readbyqxmd.com/read/29194616/comparative-analysis-of-single-cell-rna-sequencing-data-from-mouse-spermatogonial-and-mesenchymal-stem-cells-to-identify-differentially-expressed-genes-and-transcriptional-regulators-of-germline-cells
#11
Sajjad Sisakhtnezhad, Parvin Heshmati
Identifying effective internal factors for regulating germline commitment during development and for maintaining spermatogonial stem cells (SSCs) self-renewal is important to understand the molecular basis of spermatogenesis process, and to develop new protocols for the production of the germline cells from other cell sources. Therefore, this study was designed to investigate single-cell RNA-sequencing data for identification of differentially expressed genes (DEGs) in 12 mouse-derived single SSCs (mSSCs) in compare with 16 mouse-derived single mesenchymal stem cells...
December 1, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29193985/initial-germ-cell-to-somatic-cell-ratio-impacts-the-efficiency-of-ssc-expansion-in-vitro
#12
Itai Gat, Leila Maghen, Melissa Filice, Shlomit Kenigsberg, Brandon Wyse, Khaled Zohni, Peter Saraz, Andrée Gauthier Fisher, Clifford Librach
Spermatogonial Stem Cell (SSC) expansion in vitro remains a major challenge in efforts to preserve fertility among pubertal cancer survivor boys. The current study focused on innovative approaches to optimize SSC expansion. Six- to eight-week-old CD-1 murine testicular samples were harvested by mechanical and enzymatic digestion. Cell suspensions were incubated for differential plating (DP). After DP, we established two experiments comparing single vs. repetitive DP (S-DP and R-DP, respectively) until passage 2 (P2) completion...
December 1, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29181661/role-of-sertoli-and-leydig-cells-in-the-regulation-of-spermatogonial-stem-cell-and-development-of-reproductive-disorders-in-male-c57bl-6-mice-with-type-1-diabetes-mellitus
#13
E G Skurikhin, A V Pakhomova, O V Pershina, V A Krupin, N N Ermakova, E S Pan, A I Kudryashova, L A Ermolaeva, E S Khmelevskaya, V E Goldberg, V V Zhdanov, A M Dygai
Course administration streptozotocin to male C57Bl/6 mice induces a complex of symptoms typical of type 1 diabetes mellitus: hyperglycemia and insulin deficiency, focal inflammatory infiltration of the pancreas, destructive changes in the Langerhans islets, damage to the insular apparatus (reduced number of PDX1+ cells and insulin expression by the secreting cells). Male reproductive disorder are serious complications of type 1 diabetes mellitus. In "diabetic" mice, interstitial edema with inflammatory infiltration and microvascular disorders in the testicular tissue are observed, the number of endothelial precursors (CD45-/CD31+) and the total number and percentage of motile spermatozoa decreased, immature spermatogenic epithelium cells are desquamated of into the lumen of the tubules...
November 27, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29165614/long-term-health-in-recipients-of-transplanted-in-vitro-propagated-spermatogonial-stem-cells
#14
Callista L Mulder, Lisa A E Catsburg, Yi Zheng, Cindy M de Winter-Korver, Saskia K M van Daalen, Madelon van Wely, Steven Pals, Sjoerd Repping, Ans M M van Pelt
STUDY QUESTION: Is testicular transplantation of in vitro propagated spermatogonial stem cells associated with increased cancer incidence and decreased survival rates in recipient mice? SUMMARY ANSWER: Cancer incidence was not increased and long-term survival rate was not altered after transplantation of in vitro propagated murine spermatogonial stem cells (SSCs) in busulfan-treated recipients as compared to non-transplanted busulfan-treated controls. WHAT IS KNOWN ALREADY: Spermatogonial stem cell autotransplantation (SSCT) is a promising experimental reproductive technique currently under development to restore fertility in male childhood cancer survivors...
January 1, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29161703/expression-profile-of-notch3-in-mouse-spermatogonia
#15
Ryu Okada, Megumi Fujimagari, Eri Koya, Yoshikazu Hirose, Tomomi Sato, Yukio Nishina
Stable and sustainable spermatogenesis is supported by the strict regulation of self-renewal and differentiation of spermatogonial stem cells (SSC), which are a rare population of undifferentiated spermatogonia. It has been revealed that some signaling factors regulate the self-renewal of SSC; however, the molecular mechanism of SSC maintenance is still not completely understood. Notch signaling is an evolutionarily conserved juxtacrine signaling that plays important roles in the cell fate determination of various tissue stem cells...
January 22, 2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29156418/characterization-of-domestic-pig-spermatogenesis-using-spermatogonial-stem-cell-markers-in-the-early-months-of-life
#16
Julio Almunia, Kotono Nakamura, Mami Murakami, Shigeo Takashima, Masaki Takasu
In pigs, spermatogonial stem cells (SSCs), which include gonocytes and undifferentiated spermatogonia, are classically defined as being present till up to 2 months of life. However, knowledge about this transition from gonocytes to undifferentiated spermatogonia in pigs is limited. In this study, we characterized pig SSCs in neonatal (n = 6), one-month-old (1-mo-old, n = 6), two-month-old (2-mo-old, n = 6), and adult testes (n = 6) anatomically, histologically, and immunohistologically. Anatomically, testicular circumference (TC) was measured at each development stage...
November 3, 2017: Theriogenology
https://www.readbyqxmd.com/read/29143989/nanos2-promotes-differentiation-of-chicken-gallus-gallus-embryonic-stem-cells-to-male-germ-cells
#17
Zhang Wenhui, Bi Yulin, Wang Yingjie, Li Dong, He Nana, Wang Man, Jin Jing, Zuo Qisheng, Zhang Yani, Li Bichun
Nanos2 is an evolutionarily conserved RNA-binding protein containing 2 CCHC-type zinc finger motives. Here, we report that Nanos2 is strongly expressed in the testis compared to other tissues in chicken (Gallus gallus). Overexpression and knockout plasmid vectors were constructed, and in-vitro Cas9/gRNA digestion and T7 endonuclease I (T7E1) assay indicated that Nanos2-g1 possessed the highest knockout activity. In vitro and in vivo, Nanos2 overexpression accelerated the production of embryoid bodies (EBs) and SSC-like cells and promoted cvh, c-kit and integrin α6 expression...
November 16, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29136097/nonrandom-contribution-of-left-and-right-testes-to-germline-transmission-from-mouse-spermatogonial-stem-cells
#18
Mito Kanastu-Shinohara, Honda Naoki, Takashi Shinohara
Vast amounts of sperm are produced from spermatogonial stem cells (SSCs), which continuously undergo self-renewal. We examined the possible effect of laterality in male germline transmission efficiency of SSCs using a spermatogonial transplantation technique. We transplanted the same number of wild-type and Egfp transgenic SSCs in the same or different testes of individual recipient mice and compared the fertility of each type of recipient by natural mating. Transgenic mice were born within 3 months after transplantation regardless of the transplantation pattern...
November 9, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29133304/stem-cells-in-vitro-gametogenesis-and-male-fertility
#19
Go Nagamatsu, Katsuhiko Hayashi
Reconstitution in culture of biological processes, such as differentiation and organization, is a key challenge in regenerative medicine, and one in which stem cell technology plays a central role. Pluripotent stem cells (PSCs) and spermatogonial stem cells (SSCs) are useful materials for reconstitution of germ cell development in vitro, as they are capable of differentiating into gametes. Reconstitution of germ cell development, termed in vitro gametogenesis, will provide an experimental platform for a better understanding of germ cell development, as well as an alternative source of gametes for reproduction, with the potential to cure infertility...
November 13, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29113556/phospholipase-d-family-member-6-pld6-is-a-surface-marker-for-enrichment-of-undifferentiated-spermatogonia-in-the-pre-pubertal-boars
#20
Pengfei Zhang, Yuwei Qin, Yi Zheng, Wenxian Zeng
Spermatogonial stem cells (SSCs) have a functional ability to maintain self-renewal and sustain production of spermatozoa throughout the reproductive lifespan of a male. Studies on SSCs can thus better understandings of spermatogenesis and unravel the mechanisms for self-renewal and differentiation of male germline stem cells. However, the rarity of SSCs in the testis and the lack of reliable surface markers obstruct the related study and further application of SSCs. This is especially the case in livestock animals...
November 7, 2017: Stem Cells and Development
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