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spermatogonial stem cell

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https://www.readbyqxmd.com/read/28108049/expression-and-localization-of-guanine-nucleotide-binding-protein-alpha-s-in-the-testis-and-epididymis-of-rams-at-different-developmental-stages
#1
Zhen Li, Jieli Lu, Jia Chen, Quanhai Pang, Ruipeng Nan, Zhiwei Zhu
The guanine nucleotide-binding alpha S subunit (Gαs) is an important element of key signaling pathways, which is widely expressed in mammalian tissues; however, its role in the reproductive system is still unclear. In this study, we investigated the expression and localization of Gαs in the testes and epididymis of rams at different developmental stages using quantitative RT-PCR, immunohistochemistry, and western blotting. In 1-, 6-, and 12-month-old rams, the transcription of Gαs-encoding gene (Gnαs) was significantly upregulated in the corpus and cauda epididymis compared to the testes and caput epididymis (P<0...
January 15, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28107194/transdifferentiation-of-human-male-germline-stem-cells-to-hepatocytes-in-vivo-via-the-transplantation-under-renal-capsules
#2
Zheng Chen, Minghui Niu, Min Sun, Qingqing Yuan, Chencheng Yao, Jingmei Hou, Hong Wang, Liping Wen, Hongyong Fu, Fan Zhou, Zheng Li, Zuping He
Here we proposed a new concept that human spermatogonial stem cells (SSCs) can transdifferentiate into hepatocytes in vivo. We first established liver injury model of mice by carbon tetrachloride to provide proper environment for human SSC transplantation. Liver mesenchymal cells were isolated from mice and identified phenotypically. Human SSC line was recombined with liver mesenchymal cells, and they were transplanted under renal capsules of nude mice with liver injury. The grafts expressed hepatocyte hallmarks, including ALB, AAT, CK18, and CYP1A2, whereas germ cell and SSC markers VASA and GPR125 were undetected in these cells, implicating that human SSCs were converted to hepatocytes...
January 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28106276/crispr-cas9-mediated-deletion-of-c1eis-inhibits-chicken-embryonic-stem-cell-differentiation-into-male-germ-cells-gallus-gallus
#3
Qisheng Zuo, Kai Jin, Yingjie Wang, Jiuzhou Song, Yani Zhang, Bichun Li
We previously found that C1EIS is preferentially expressed in Chicken spermatogonial stem cells (SSCs) by RNA sequencing (RNA-seq), so our current study focused on C1EIS's role in Chicken embryonic stem cells (ESCs) differentiation into male germ cells. We constructed a CRISPR/Cas9 vector targeting C1EIS. T7 endonuclease I (T7EI) digestion method and sequencing of TA cloning were used to detect the knock-out efficiency of the Single guide RNA (sgRNA) after the cas9/gRNA vector transfected into D fibroblasts 1(DF-1), ESCs and Chicken embryos...
January 20, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28093458/single-cell-gene-expression-analysis-reveals-diversity-among-human-spermatogonia
#4
N Neuhaus, J Yoon, N Terwort, S Kliesch, J Seggewiss, A Huge, R Voss, S Schlatt, R V Grindberg, H R Schöler
STUDY QUESTION: Is the molecular profile of human spermatogonia homogeneous or heterogeneous when analysed at the single-cell level? SUMMARY ANSWER: Heterogeneous expression profiles may be a key characteristic of human spermatogonia, supporting the existence of a heterogeneous stem cell population. WHAT IS KNOWN ALREADY: Despite the fact that many studies have sought to identify specific markers for human spermatogonia, the molecular fingerprint of these cells remains hitherto unknown...
January 15, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28091913/regenerative-potential-of-stem-and-progenitor-cells-from-ischemic-testes-of-c57bl-6-mice-in-culture-and-in-the-model-of-spermatogenesis-suppression-caused-by-busulfan
#5
E G Skurikhin, A V Pakhomova, O V Pershina, L A Ermolaeva, N N Ermakova, V A Krupin, E S Pan, A I Kudryashova, O Yu Rybalkina, V V Zhdanov, V E Goldberg, A M Dygai
The regenerative potential of stem and progenitor cells from ischemic testes of C57Bl/6 mice was studied in vitro (cell culture) and in vivo (mouse model of busulfan-induced suppression of spermatogenesis). Spermatogonial stem cells with phenotypes CD117(-)CD90(+) and CD51(-)CD24(+)CD52(+) from ischemic testes demonstrated 33-fold and 7-fold increments of cell mass and generated colonies in vitro. Epithelial (CD45(-)CD31(-)Sca-1(+)CD49f(+)) and endothelial (CD45(-)CD31(+)) precursors exhibited lower self-renewal capacity...
January 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28087628/id4-levels-dictate-the-stem-cell-state-in-mouse-spermatogonia
#6
Aileen R Helsel, Qi-En Yang, Melissa J Oatley, Tessa Lord, Fred Sablitzky, Jon M Oatley
Spermatogenesis is a classic model of cycling cell lineages that depend on a balance between stem cell self-renewal for continuity and formation of progenitors as the initial step in production of differentiated cells. However, the mechanisms guiding the continuum of spermatogonial stem cell (SSC) to progenitor spermatogonial transition and precise identifiers of subtypes in the process are undefined. Here we used an Id4-eGfp reporter mouse to discover that EGFP intensity is predictive of the subsets with the ID4-EGFP(Bright) population being mostly, if not purely, SSCs while the ID4-EGFP(Dim) population is in transition to the progenitor state...
January 13, 2017: Development
https://www.readbyqxmd.com/read/28077131/granulocyte-colony-stimulating-factor-g-csf-promotes-spermatogenic-regeneration-from-surviving-spermatogonia-after-high-dose-alkylating-chemotherapy
#7
Travis Kotzur, Roberto Benavides-Garcia, Jennifer Mecklenburg, Jamila R Sanchez, Matthew Reilly, Brian P Hermann
BACKGROUND: The lifesaving chemotherapy and radiation treatments that allow patients to survive cancer can also result in a lifetime of side-effects, including male infertility. Infertility in male cancer survivors is thought to primarily result from killing of the spermatogonial stem cells (SSCs) responsible for producing spermatozoa since SSCs turn over slowly and are thereby sensitive to antineoplastic therapies. We previously demonstrated that the cytokine granulocyte colony-stimulating factor (G-CSF) can preserve spermatogenesis after alkylating chemotherapy (busulfan)...
January 11, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28071722/spict-a-cyst-cell-specific-gene-regulates-starvation-induced-spermatogonial-cell-death-in-the-drosophila-testis
#8
Ason C-Y Chiang, Heiko Yang, Yukiko M Yamashita
Tissues are maintained in a homeostatic state by balancing the constant loss of old cells with the continued production of new cells. Tissue homeostasis can shift between high and low turnover states to cope with environmental changes such as nutrient availability. Recently, we discovered that the elimination of transit-amplifying cells plays a critical role in maintaining the stem cell population during protein starvation in the Drosophila testis. Here, we identify spict, a gene expressed specifically in differentiating cyst cells, as a regulator of spermatogonial death...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28071690/generation-of-germline-ablated-male-pigs-by-crispr-cas9-editing-of-the-nanos2-gene
#9
Ki-Eun Park, Amy V Kaucher, Anne Powell, Muhammad Salman Waqas, Shelley E S Sandmaier, Melissa J Oatley, Chi-Hun Park, Ahmed Tibary, David M Donovan, Le Ann Blomberg, Simon G Lillico, C Bruce A Whitelaw, Alan Mileham, Bhanu P Telugu, Jon M Oatley
Genome editing tools have revolutionized the generation of genetically modified animals including livestock. In particular, the domestic pig is a proven model of human physiology and an agriculturally important species. In this study, we utilized the CRISPR/Cas9 system to edit the NANOS2 gene in pig embryos to generate offspring with mono-allelic and bi-allelic mutations. We found that NANOS2 knockout pigs phenocopy knockout mice with male specific germline ablation but other aspects of testicular development are normal...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28070774/restoring-fertility-with-cryopreserved-prepubertal-testicular-tissue-perspectives-with-hydrogel-encapsulation-nanotechnology-and-bioengineered-scaffolds
#10
Maxime Vermeulen, Jonathan Poels, Francesca de Michele, Anne des Rieux, Christine Wyns
New and improved oncological therapies are now able to cure more than 80% of cancer-affected children in Europe. However, such treatments are gonadotoxic and result in fertility issues, especially in boys who are not able to provide a sperm sample before starting chemo/radiotherapy because of their prepubertal state. For these boys, cryopreservation of immature testicular tissue (ITT) is the only available option, aiming to preserve spermatogonial stem cells (SSCs). Both slow-freezing and vitrification have been investigated to this end and are now applied in a clinical setting for SSC cryopreservation...
January 9, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28065642/mesenchymal-to-epithelial-transition-mediated-by-cdh1-promotes-spontaneous-reprogramming-of-male-germline-stem-cells-to-pluripotency
#11
Junhui An, Yu Zheng, Christina Tenenhaus Dann
Cultured spermatogonial stem cells (GSCs) can spontaneously form pluripotent cells in certain culture conditions. However, GSC reprogramming is a rare event that is largely unexplained. We show GSCs have high expression of mesenchymal to epithelial transition (MET) suppressors resulting in a developmental barrier inhibiting GSC reprogramming. Either increasing OCT4 or repressing transforming growth factor β (TGF-β) signaling promotes GSC reprogramming by upregulating CDH1 and boosting MET. Reducing ZEB1 also enhances GSC reprogramming through its direct effect on CDH1...
December 24, 2016: Stem Cell Reports
https://www.readbyqxmd.com/read/28053620/establishment-of-a-surgically-induced-cryptorchidism-canine-recipient-model-for-spermatogonial-stem-cell-transplantation
#12
Won-Young Lee, Ran Lee, Hyuk Song, Tai-Young Hur, Seunghoon Lee, Jiyun Ahn, Hyunjhung Jhun
Transplantation of spermatogonial stem cells (SSCs) in experimental animal models has been used to study germ line stem cell biology and to produce transgenic animals. The species-specific recipient model preparation is important for the characterization of SSCs and the production of offspring. Here, we investigated the effects of surgically induced cryptorchidism in dog as a new recipient model for spermatogonial stem cell transplantation. Artificially unilateral or bilateral cryptorchidism was induced in ten mature male dogs by surgically returning the testis and epididymis to the abdominal cavity...
December 2016: Laboratory Animal Research
https://www.readbyqxmd.com/read/28052023/spermatogonial-stem-cells-and-progenitors-are-refractory-to-reprogramming-to-pluripotency-by-the-transcription-factors-oct3-4-c-myc-sox2-and-klf4
#13
Sébastien Corbineau, Bruno Lassalle, Maelle Givelet, Inès Souissi-Sarahoui, Virginie Firlej, Paul Henri Romeo, Isabelle Allemand, Lydia Riou, Pierre Fouchet
The male germinal lineage, which is defined as unipotent, produces sperm through spermatogenesis. However, embryonic primordial germ cells and postnatal spermatogonial stem cells (SSCs) can change their fate and convert to pluripotency in culture when they are not controlled by the testicular microenvironment. The mechanisms underlying these reprogramming processes are poorly understood. Testicular germ cell tumors, including teratoma, share some molecular characteristics with pluripotent cells, suggesting that cancer could result from an abnormal differentiation of primordial germ cells or from an abnormal conversion of SCCs to pluripotency in the testis...
December 28, 2016: Oncotarget
https://www.readbyqxmd.com/read/28051360/the-notch-ligand-jag1-regulates-gdnf-expression-in-sertoli-cells
#14
Thomas Xavier Garcia, Parag Parekh, Pooja Gandhi, Krishna M Sinha, Marie-Claude Hofmann
In the seminiferous epithelium of the testis, Sertoli cells are key niche cells directing proliferation and differentiation of spermatogonial stem cells (SSCs) into spermatozoa. Sertoli cells produce glial cell line-derived neurotrophic factor (GDNF), which is essential for SSC self-renewal and progenitor expansion. While the role of GDNF in the testis stem cell niche is established, little is known about how this factor is regulated. Our previous studies on NOTCH activity in Sertoli cells demonstrated a role of this pathway in limiting stem/progenitor cell numbers, thus ultimately down-regulating sperm cell output...
January 4, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28040413/in-search-of-an-improved-injection-technique-for-the-clinical-application-of-spermatogonial-stem-cell-transplantation
#15
Katrien Faes, Tony Lahoutte, Anne Hoorens, Herman Tournaye, Ellen Goossens
When fertility is impaired by anticancer treatment, spermatogonial stem cell transplantation (SSCT) could be used as a fertility restoration technique later on in life. Previously, we have demonstrated that a testicular cell suspension could be injected into a human cadaver testis, however, leakage to the interstitium was observed. In this study, injection of mouse testicular cells at an injection height of 50 cm (hydrostatic pressure) or via an automated injection pump (1400 µl, 2600 µl and 3000 µl) was evaluated...
December 15, 2016: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28028909/regulation-of-injury-induced-ovarian-regeneration-by-activation-of-oogonial-stem-cells
#16
Piril Erler, Alexandra Sweeney, James R Monaghan
Some animals have the ability to generate large numbers of oocytes throughout life. This raises the question whether persistent adult germline stem cell populations drive continuous oogenesis and whether they are capable of mounting a regenerative response after injury. Here we demonstrate the presence of adult oogonial stem cells (OSCs) in the adult axolotl salamander ovary and show that ovarian injury induces OSC activation and functional regeneration of the ovaries to reproductive capability. Cells that have morphological similarities to germ cells were identified in the developing and adult ovaries via histological analysis...
January 2017: Stem Cells
https://www.readbyqxmd.com/read/28027652/melatonin-ameliorates-busulfan-induced-spermatogonial-stem-cell-oxidative-apoptosis-in-mouse-testes
#17
Bo Li, Xin He, Mengru Zhuang, Bowen Niu, Chongyang Wu, Hailong Mu, Furong Tang, Yanhua Cui, Weishuai Liu, Baoyu Zhao, Sha Peng, Guangpeng Li, Jinlian Hua
AIMS: Many men endure immunosuppressive or anticancer treatments that contain alkylating agents prior to the age of sexual maturity, especially the increasing number of preadolescent males who undergo busulfan treatment for myeloablative conditioning prior to hematopoietic stem cell transplantation. Prior to sperm production, there are no sperm available for cryopreservation. Thus, it is necessary to identify a solution to ameliorate the busulfan-induced damage of spermatogonial stem cells (SSCs)...
December 27, 2016: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28017655/cd59-regulation-by-sox2-is-required-for-epithelial-cancer-stem-cells-to-evade-complement-surveillance
#18
Jianfeng Chen, Peipei Ding, Ling Li, Hongyu Gu, Xin Zhang, Long Zhang, Na Wang, Lu Gan, Qi Wang, Wei Zhang, Weiguo Hu
Cancer stem cells (CSCs) are highly associated with therapy resistance and metastasis. Interplay between CSCs and various immune components is required for tumor survival. However, the response of CSCs to complement surveillance remains unknown. Herein, using stem-like sphere-forming cells prepared from a mammary tumor and a lung adenocarcinoma cell line, we found that CD59 was upregulated to protect CSCs from complement-dependent cytotoxicity. CD59 silencing significantly enhanced complement destruction and completely suppressed tumorigenesis in CSC-xenografted nude mice...
January 10, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28008926/long-term-propagation-of-tree-shrew-spermatogonial-stem-cells-in-culture-and-successful-generation-of-transgenic-offspring
#19
Chao-Hui Li, Lan-Zhen Yan, Wen-Zan Ban, Qiu Tu, Yong Wu, Lin Wang, Rui Bi, Shuang Ji, Yu-Hua Ma, Wen-Hui Nie, Long-Bao Lv, Yong-Gang Yao, Xu-Dong Zhao, Ping Zheng
Tree shrews have a close relationship to primates and have many advantages over rodents in biomedical research. However, the lack of gene manipulation methods has hindered the wider use of this animal. Spermatogonial stem cells (SSCs) have been successfully expanded in culture to permit sophisticated gene editing in the mouse and rat. Here, we describe a culture system for the long-term expansion of tree shrew SSCs without the loss of stem cell properties. In our study, thymus cell antigen 1 was used to enrich tree shrew SSCs...
December 23, 2016: Cell Research
https://www.readbyqxmd.com/read/28007786/myc-mycn-mediated-glycolysis-enhances-mouse-spermatogonial-stem-cell-self-renewal
#20
Mito Kanatsu-Shinohara, Takashi Tanaka, Narumi Ogonuki, Atsuo Ogura, Hiroko Morimoto, Pei Feng Cheng, Robert N Eisenman, Andreas Trumpp, Takashi Shinohara
Myc plays critical roles in the self-renewal division of various stem cell types. In spermatogonial stem cells (SSCs), Myc controls SSC fate decisions because Myc overexpression induces enhanced self-renewal division, while depletion of Max, a Myc-binding partner, leads to meiotic induction. However, the mechanism by which Myc acts on SSC fate is unclear. Here we demonstrate a critical link between Myc/Mycn gene activity and glycolysis in SSC self-renewal. In SSCs, Myc/Mycn are regulated by Foxo1, whose deficiency impairs SSC self-renewal...
December 1, 2016: Genes & Development
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