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https://www.readbyqxmd.com/read/28928476/bisphenol-a-affects-on-the-functional-properties-and-proteome-of-testicular-germ-cells-and-spermatogonial-stem-cells-in-vitro-culture-model
#1
Polash Chandra Karmakar, Hyun-Gu Kang, Yong-Hee Kim, Sang-Eun Jung, Md Saidur Rahman, Hee-Seok Lee, Young-Hyun Kim, Myung-Geol Pang, Buom-Yong Ryu
The endocrine disruptor bisphenol A (BPA) is well known for its adverse effect on male fertility. Growing evidence suggests that BPA may interact with testicular germ cells and cause infertility as a result of its estrogenic activity. Objective of current in vitro study was to investigate the proliferation, survivability and stemness properties of mouse testicular germ cells exposed to BPA, and to evaluate possible expression of cellular proteome. Our results showed that germ cell viability and proliferation were not affected by low concentrations (0...
September 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28927307/biological-therapy-for-non-obstructive-azoospermia
#2
Sarah C Vij, Edmund Sabanegh, Ashok Agarwal
Most male patients with non-obstructive azoospermia (NOA) have no therapeutic options outside of assisted reproductive techniques to conceive a biological child. If mature sperm cannot be obtained from the testes, these patients must rely on options of donor sperm or adoption. Several techniques are in the experimental stage to provide this patient population alternatives for conceiving. Areas covered: This review discusses three of the experimental techniques for restoring fertility in men with NOA: spermatogonial stem cell transplantation, the use of adult and embryonic stem cells to develop mature gametes and gene therapy...
September 19, 2017: Expert Opinion on Biological Therapy
https://www.readbyqxmd.com/read/28919176/different-approaches-to-establish-infertile-rooster
#3
Fereshteh Ghadimi, Malak Shakeri, Mahdi Zhandi, Mojtaba Zaghari, Abbas Piryaei, Parham Moslehifar, Alireza Rajabinejad
Several methods have been developed to suppress spermatogenesis in recipient males before spermatogonial stem cells (SSCs) transplantation. The aim of this study was to compare two different methods of depleting endogenous spermatogenesis in recipient ROSS 308 strain adult roosters. Gamma-radiation and alkylating agent busulfan were utilized to infertilize adult roosters (ROSS 308 strain). Two radiation therapy regimes (based on (60)co isotope) were conducted locally to testes using 40Gy (5×8Gy with three-day intervals) and 30Gy (3×10Gy with three-day intervals)...
August 26, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28914256/mettl3-mettl14-mediated-mrna-n-6-methyladenosine-modulates-murine-spermatogenesis
#4
Zhen Lin, Phillip J Hsu, Xudong Xing, Jianhuo Fang, Zhike Lu, Qin Zou, Ke-Jia Zhang, Xiao Zhang, Yuchuan Zhou, Teng Zhang, Youcheng Zhang, Wanlu Song, Guifang Jia, Xuerui Yang, Chuan He, Ming-Han Tong
Spermatogenesis is a differentiation process during which diploid spermatogonial stem cells (SSCs) produce haploid spermatozoa. This highly specialized process is precisely controlled at the transcriptional, posttranscriptional, and translational levels. Here we report that N(6)-methyladenosine (m(6)A), an epitranscriptomic mark regulating gene expression, plays essential roles during spermatogenesis. We present comprehensive m(6)A mRNA methylomes of mouse spermatogenic cells from five developmental stages: undifferentiated spermatogonia, type A1 spermatogonia, preleptotene spermatocytes, pachytene/diplotene spermatocytes, and round spermatids...
September 15, 2017: Cell Research
https://www.readbyqxmd.com/read/28910977/bisphenol-a-induced-apoptosis-and-transcriptome-differences-of-spermatogonial-stem-cells-in-vitro
#5
Xiaowen Gong, Hui Xie, Xiaoyong Li, Ji Wu, Yi Lin
Bisphenol A (BPA) is widely used as an industrial plasticizer, which is also an endocrine disruptor and considered to have adverse effects on reproduction. In male mammals, the long-term production of billions of spermatozoa relies on the regulated proliferation and differentiation of spermatogonial stem cells (SSCs). However, little is known about the effects of BPA on the viability of SSCs. To investigate the influence of BPA exposure on SSCs in vitro, we isolated SSCs from mouse and successfully established in vitro propagation of SSCs...
September 1, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28900261/a-phytochemical-approach-to-promotion-of-self-renewal-in-murine-spermatogonial-stem-cell-by-using-sedum-sarmentosum-extract
#6
Sang-Eun Jung, Yong-Hee Kim, Sunghun Cho, Bang-Jin Kim, Hee-Seok Lee, Seongsoo Hwang, Geun-Bae Kim, Young-Hyun Kim, Myung-Geol Pang, Sanghyun Lee, Buom-Yong Ryu
Spermatogonial stem cells (SSCs) are the basis of spermatogenesis, which is dependent on the ability to self-renew and differentiation. Controlling self-renewal and differentiation of SSCs could apply to treatment of disease such as male infertility. Recently, in the field of stem cell research, it was demonstrated that effective increase in stem cell activity can be achieved by using growth factors derived from plant extracts. In this study, our aim is to investigate components from natural plant to improve the self-renewal of SSCs...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28898437/regulation-of-fibroblast-growth-factor-8-fgf8-in-chicken-embryonic-stem-cells-differentiation-into-spermatogonial-stem-cells
#7
Man Wang, Chen Zhang, Chuanli Huang, Shaoze Cheng, Nana He, Yilin Wang, Mahmoud F Ahmed, Ruifeng Zhao, Jing Jin, Qisheng Zuo, Yani Zhang, Bichun Li
Fibroblast growth factors (FGFs) are essential in regulating the formation of spermatogonial stem cells (SSCs). Here, we explored the effect of FGF8 on chicken SSCs formation by knockdown or overexpression of FGF8 in chicken embryonic stem cells (ESCs) both in vitro and in vivo. Our results showed that knockdown of FGF8 could facilitate the differentiation of ESCs into SSCs, overexpression of FGF8 could promote PGCs self-renewal, inhibit SSCs formation. This study further revealed the positive correlation between the expression level of FGF8 and MAPK/ERK signal...
September 12, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28890329/histone-methyltransferase-setdb1-maintains-survival-of-mouse-spermatogonial-stem-progenitor-cells-via-pten-akt-foxo1-pathway
#8
Tiantian Liu, Xiaoxu Chen, Tianjiao Li, Xueliang Li, Yinghua Lyu, Xiaoteng Fan, Pengfei Zhang, Wenxian Zeng
Spermatogonial stem cells (SSCs) possess the capacity of self-renewal and differentiation, which are the basis of spermatogenesis. In maintenance of SSC homeostasis, intrinsic/extrinsic factors and various signaling pathways tightly control the fate of SSCs. Methyltransferase SETDB1 (Set domain, bifurcated 1) catalyzes histone H3 lysine 9 (H3K9) trimethylation and represses gene expression. SETDB1 is required for maintaining the survival of spermatogonial stem cells in mice. However, the underlying molecular mechanism remains unclear...
September 7, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28886879/analysis-of-putative-biomarkers-of-undifferentiated-spermatogonia-in-dog-testis
#9
Won-Young Lee, Hyun-Jung Park, Ran Lee, Ji-Heon Lee, Hyunjhung Jhun, Tai-Young Hur, Hyuk Song
Spermatogenesis begins after puberty and continues throughout a male's life, and is regulated by spermatogonial stem cells in the seminiferous tubules. Markers of male germ cells, including undifferentiated spermatogonia to fully developed spermatozoa have been identified in rodents, but not in dogs. In this study, to characterize the markers of undifferentiated spermatogonia, histological and immunohistochemical analyses were performed on pre-pubertal (1-month-old), early pubertal (4-month-old), and post-pubertal (7-month-old) dog testes...
October 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28883837/role-of-the-hedgehog-signaling-pathway-in-regulating-the-behavior-of-germline-stem-cells
#10
REVIEW
Shiqin Li, Meng Wang, Yanghui Chen, Wei Wang, Junying Wu, Chengpeng Yu, Yuehui Zheng, Zezheng Pan
Germline stem cells (GSCs) are adult stem cells that are responsible for the production of gametes and include spermatogonial stem cells (SSCs) and ovarian germline stem cells (OGSCs). GSCs are located in a specialized microenvironment in the gonads called the niche. Many recent studies have demonstrated that multiple signals in the niche jointly regulate the proliferation and differentiation of GSCs, which is of significance for reproductive function. Previous studies have demonstrated that the hedgehog (Hh) signaling pathway participates in the proliferation and differentiation of various stem cells, including GSCs in Drosophila and male mammals...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28867346/germline-stem-cell-activity-is-sustained-by-sall4-dependent-silencing-of%C3%A2-distinct-tumor-suppressor-genes
#11
Ai-Leen Chan, Hue M La, Julien M D Legrand, Juho-Antti Mäkelä, Michael Eichenlaub, Mia De Seram, Mirana Ramialison, Robin M Hobbs
Sustained spermatogenesis in adult males and fertility recovery following germ cell depletion are dependent on undifferentiated spermatogonia. We previously demonstrated a key role for the transcription factor SALL4 in spermatogonial differentiation. However, whether SALL4 has broader roles within spermatogonia remains unclear despite its ability to co-regulate genes with PLZF, a transcription factor required for undifferentiated cell maintenance. Through development of inducible knockout models, we show that short-term integrity of differentiating but not undifferentiated populations requires SALL4...
September 12, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28851723/the-nature-and-dynamics-of-spermatogonial-stem-cells
#12
REVIEW
Dirk G de Rooij
Spermatogonial stem cells (SSCs) are crucial for maintaining spermatogenesis throughout life, and understanding how these cells function has important implications for understanding male infertility. Recently, various populations of cells harbouring stem cell-like properties have been identified in rodent seminiferous tubules, but deciphering how these cells might fuel spermatogenesis has been difficult, and various models to explain SSC dynamics have been put forward. This Review provides an overview of the organization and timing of spermatogenesis and then discusses these models in light of recent studies of SSC markers, heterogeneity and cell division dynamics, highlighting the evidence for and against each model...
September 1, 2017: Development
https://www.readbyqxmd.com/read/28846448/in-vitro-effects-of-melatonin-on-colonization-of-neonate-mouse-spermatogonial-stem-cells
#13
Shadan Navid, Tayebeh Rastegar, Maryam Baazm, Rafieh Alizadeh, Ali Talebi, Keykavos Gholami, Somayeh Khosravi-Farsani, Morteza Koruji, Mehdi Abbasi
We have recently reported that antioxidant supplements enhance the efficacy of cryopreserved spermatogonial stem cells. Melatonin is considered a free radical scavenger which has direct and indirect antioxidant effects in in vitro and in vivo microenvironments. Due to the anti-apoptotic properties of melatonin, researchers have proposed that melatonin may improve the efficiency of spermatogonial stem cell (SSC) transplantation. However, the appropriate methodology which facilitates SSC proliferation remains to be determined...
August 28, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28837026/a-comparison-between-a-new-vitrification-protocol-and-the-slow-freezing-method-in-the-cryopreservation-of-prepubertal-testicular-tissue
#14
Moacir R M Radaelli, Carlos G Almodin, Vânia C Minguetti-Câmara, Paula Motta Almodin Cerialli, Aissar E Nassif, Antonio J Gonçalves
OBJECTIVE: This study aimed to compare a new vitrification protocol with reduced cryoprotectant exposure to the slow freezing method in the cryopreservation of prepubertal rat testicular tissue. METHODS: Five sexually immature male Wistar rats were submitted to bilateral orchiectomy. Tissue samples from each testicle were fragmented into small pieces and randomly assigned to three groups: Group A, fresh tissue (control); Group B, slow programmable freezing (SPF); and Group C (vitrification)...
September 1, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28836968/infrarenal-high-intra-abdominal-testis-fusion-of-t2-weighted-and-diffusion-weighted-magnetic-resonance-images-and-pathological-findings
#15
Seiji Hoshi, Yuichi Sato, Junya Hata, Hidenori Akaihata, Soichiro Ogawa, Nobuhiro Haga, Yoshiyuki Kojima
BACKGROUND: Several recent reports have demonstrated that the preoperative sensitivity and accuracy of identifying and locating non-palpable testes increases with the use of conventional MRI, in addition to diffusion-weighted imaging (DWI). Therefore, pre-operative prediction of the presence and location of testes using imaging techniques may guide management of intra-abdominal testis. Fowler-Stephens orchiopexy is effective for treating patients with intra-abdominal testis; however, long-term testicular function after this procedure has not been clarified...
August 24, 2017: BMC Urology
https://www.readbyqxmd.com/read/28834365/spontaneous-testicular-atrophy-occurs-despite-normal-spermatogonial-proliferation-in-a-tp53-knockout-rat
#16
M S Dai, S J Hall, M M Vantangoli Policelli, K Boekelheide, D J Spade
The tumor suppressor protein p53 (TP53) has many functions in cell cycle regulation, apoptosis, and DNA damage repair and is also involved in spermatogenesis in the mouse. To evaluate the role of p53 in spermatogenesis in the rat, we characterized testis biology in adult males of a novel p53 knockout rat (SD-Tp53(tm1sage) ). p53 knockout rats exhibited variable levels of testicular atrophy, including significantly decreased testis weights, atrophic seminiferous tubules, decreased seminiferous tubule diameter, and elevated spermatocyte TUNEL labeling rates, indicating a dysfunction in spermatogenesis...
August 22, 2017: Andrology
https://www.readbyqxmd.com/read/28831567/horse-spermatogonial-stem-cell-cryopreservation-feasible-protocols-and-potential-biotechnological-applications
#17
Guilherme M J Costa, Gleide F Avelar, Samyra M S N Lacerda, André F A Figueiredo, Amanda O Tavares, José V Rezende-Neto, Felipe G P Martins, Luiz R França
The establishment of proper conditions for spermatogonial stem cells (SSCs) cryopreservation and storage represents an important biotechnological approach for the preservation of the genetic stock of valuable animals. This study demonstrates the effects of different cryopreservation protocols on the survival rates and phenotypic expression of SSCs in horses. The cells were enzymatically isolated from testes of eight adult horses. After enrichment and characterization of germ cells in the suspension, the feasibility of several cryopreservation protocols were evaluated...
August 22, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28827392/spermatogonial-kinetics-in-the-human
#18
Sara Di Persio, Rossana Saracino, Stefania Fera, Barbara Muciaccia, Valentina Esposito, Carla Boitani, Bartolomeo P Berloco, Francesco Nudo, Gustavo Spadetta, Mario Stefanini, Dirk G de Rooij, Elena Vicini
The human spermatogonial compartment is essential for daily production of millions of sperm. Despite this crucial role, the molecular signature, kinetic behavior and regulation of human spermatogonia are poorly understood. Using human testis biopsies with normal spermatogenesis and studying marker protein expression, for the first time we identified different subpopulations of spermatogonia. MAGE-A4 marks all spermatogonia, KIT marks all B spermatogonia and UCLH1 all Apale-dark (Ap-d) spermatogonia. We suggest that at the start of the spermatogenic lineage there are Ap-d spermatogonia that are GFRA1(High) likely including the spermatogonial stem cells...
August 21, 2017: Development
https://www.readbyqxmd.com/read/28816413/porcine-spermatogonial-stem-cells-self-renew-effectively-in-a-three-dimensional-culture-microenvironment
#19
Ji Eun Park, Min Hee Park, Min Seong Kim, Yeo Reum Park, Jung Im Yun, Hee Tae Cheong, Minseok Kim, Jung Hoon Choi, Eunsong Lee, Seung Tae Lee
Generally, self-renewal of spermatogonial stem cells (SSCs) is maintained in vivo in a three-dimensional (3D) microenvironment consisting of the seminiferous tubule basement membrane, indicating the importance of the 3D microenvironment for in vitro culture of SSCs. Here, we report a 3D culture microenvironment that effectively maintains porcine SSC self-renewal during culture. Porcine SSCs were cultured in an agarose-based 3D hydrogel and in 2D culture plates either with or without feeder cells. Subsequently, the effects of 3D culture on the maintenance of undifferentiated SSCs were identified by analyzing cell colony formation and morphology, AP activity, and transcriptional and translational regulation of self-renewal-related genes and the effects on proliferation by analyzing cell viability and single cell-derived colony number...
August 17, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28815778/mapk8-regulates-chicken-male-germ-cell-differentiation-through-jnk-signaling-pathway
#20
Yingjie Wang, Yulin Bi, Qisheng Zuo, Wenhui Zhang, Dong Li, Na-Na He, Shaoze Cheng, Ya-Ni Zhang, Bichun Li
The study aims to analyze the key signaling pathways in regulating the process of embryonic stem cells (ESCs) differentiation into spermatogonial stem cells (SSCs). We explored the specific regulating mechanisms of C-Jun amino-terminal kinase (JNK) signaling in this process. Interference/overexpression of MAPK8 allows the JNK signaling pathway to be blocked/activated. In Retinoic acid (RA) induced in vitro differentiation assays, the expression of germ cell marker genes, cvh, c-kit, integrin α6 and integrin β1, was observed to upregulate while activating JNK signaling significantly...
August 16, 2017: Journal of Cellular Biochemistry
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