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https://www.readbyqxmd.com/read/29143989/nanos2-promotes-differentiation-of-chicken-gallus-gallus-embryonic-stem-cells-to-male-germ-cells
#1
Zhang Wenhui, Bi Yulin, Wang Yingjie, Li Dong, He Nana, Wang Man, Jin Jing, Zuo Qisheng, Zhang Yani, Li Bichun
Nanos2 is an evolutionarily conserved RNA-binding protein containing 2 CCHC-type zinc finger motives. Here, we report that Nanos2 is strongly expressed in the testis compared to other tissues in chicken (Gallus gallus). Overexpression and knockout plasmid vectors were constructed, and in-vitro Cas9/gRNA digestion and T7 endonuclease I (T7E1) assay indicated that Nanos2-g1 possessed the highest knockout activity. In vitro and in vivo, Nanos2 overexpression accelerated the production of embryoid bodies (EBs) and SSC-like cells and promoted cvh, c-kit and integrin α6 expression...
November 16, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29136097/nonrandom-contribution-of-left-and-right-testes-to-germline-transmission-from-mouse-spermatogonial-stem-cells
#2
Mito Kanastu-Shinohara, Honda Naoki, Takashi Shinohara
Vast amounts of sperm are produced from spermatogonial stem cells (SSCs), which continuously undergo self-renewal. We examined the possible effect of laterality in male germline transmission efficiency of SSCs using a spermatogonial transplantation technique. We transplanted the same number of wild-type and Egfp transgenic SSCs in the same or different testes of individual recipient mice and compared the fertility of each type of recipient by natural mating. Transgenic mice were born within 3 months after transplantation regardless of the transplantation pattern...
November 9, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29133304/stem-cells-in-vitro-gametogenesis-and-male-fertility
#3
Go Nagamatsu, Katsuhiko Hayashi
Reconstitution in culture of biological processes, such as differentiation and organization, is a key challenge in regenerative medicine, and one in which stem cell technology plays a central role. Pluripotent stem cells (PSCs) and spermatogonial stem cells (SSCs) are useful materials for reconstitution of germ cell development in vitro, as they are capable of differentiating into gametes. Reconstitution of germ cell development, termed in vitro gametogenesis, will provide an experimental platform for a better understanding of germ cell development, as well as an alternative source of gametes for reproduction, with the potential to cure infertility...
November 13, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29113556/phospholipase-d-family-member-6-pld6-is-a-surface-marker-for-enrichment-of-undifferentiated-spermatogonia-in-the-pre-pubertal-boars
#4
Pengfei Zhang, Yuwei Qin, Yi Zheng, Wenxian Zeng
Spermatogonial stem cells (SSCs) have a functional ability to maintain self-renewal and sustain production of spermatozoa throughout the reproductive lifespan of a male. Studies on SSCs can thus better understandings of spermatogenesis and unravel the mechanisms for self-renewal and differentiation of male germline stem cells. However, the rarity of SSCs in the testis and the lack of reliable surface markers obstruct the related study and further application of SSCs. This is especially the case in livestock animals...
November 7, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29109271/periodic-production-of-retinoic-acid-by-meiotic-and-somatic-cells-coordinates-four-transitions-in-mouse-spermatogenesis
#5
Tsutomu Endo, Elizaveta Freinkman, Dirk G de Rooij, David C Page
Mammalian spermatogenesis is an elaborately organized differentiation process, starting with diploid spermatogonia, which include germ-line stem cells, and ending with haploid spermatozoa. The process involves four pivotal transitions occurring in physical proximity: spermatogonial differentiation, meiotic initiation, initiation of spermatid elongation, and release of spermatozoa. We report how the four transitions are coordinated in mice. Two premeiotic transitions, spermatogonial differentiation and meiotic initiation, were known to be coregulated by an extrinsic signal, retinoic acid (RA)...
November 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29107344/faithful-artificial-chromosome-propagation-using-spermatogonial-stem-cells
#6
M Yamada, M Seandel
Artificial chromosomes are useful in making functional vectors for very large genes, studying chromosome physiology, and modeling chromosomal disorders. Shinohara et al. have succeeded for the first time in creating transchromosomic mice by manipulating spermatogonial stem cells (SSCs), which exhibited superior chromosomal stability compared with embryonic stem cells (ESCs).
October 26, 2017: Trends in Genetics: TIG
https://www.readbyqxmd.com/read/29105399/upregulation-of-integrin-%C3%AE-6-and-integrin-%C3%AE-1-gene-expressions-in-mouse-spermatogonial-stem-cells-after-continues-and-pulsed-low-intensity-ultrasound-stimulation
#7
Mahdi Mohaqiq, Mansoureh Movahedin, Manijhe Mokhtari Dizaji, Zohreh Mazaheri
OBJECTIVES: low intensity ultrasound (continues and pulsed) is a form of energy. Spermatogonial stem cells (SSCs) are at the base of male fertility. This study investigated the effects of low intensity ultrasound stimulation (LIUS) and low intensity pulsed ultrasound stimulation (LIUPS) on the expression of germ cell-specific and pluripotency genes in SSCs in vitro. MATERIALS AND METHODS: In this experimental study, isolated SSCs from neonatal male mice were cultured in Dulbecco's Modified Eagle's Medium (DMEM) with 10% fetal bovine serum (FBS)...
January 2018: Cell Journal
https://www.readbyqxmd.com/read/29063123/cadherin-22-participates-in-the-self-renewal-of-mouse-female-germ-line-stem-cells-via-interaction-with-jak2-and-%C3%AE-catenin
#8
Xiaoyu Zhang, Yang Yang, Qin Xia, Hongfei Song, Rui Wei, Jingjing Wang, Kang Zou
The self-renewal capacity of the stem cell pool determines tissue function and health. Cadherin-22 (Cdh22), a member of the cadherin superfamily, has two splicing patterns in rats, and the short type that lacks a catenin binding domain is closely related to spermatogonial stem cell self-renewal. Previously, we reported that CDH22 was highly expressed in mouse ovary germ cells, especially in female germ line stem cells (FGSCs). However, its underlying function in FGSCs is still not clear. Here, we found that Cdh22 encodes only one type of protein product in mice and demonstrated that CDH22 was required for FGSC self-renewal...
October 24, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29057252/glis1-3-emerging-roles-in-reprogramming-stem-and-progenitor-cell-differentiation-and-maintenance
#9
REVIEW
David W Scoville, Hong Soon Kang, Anton M Jetten
Recent studies have provided evidence for a regulatory role of GLI-similar (GLIS) transcription factors in reprogramming, maintenance and differentiation of several stem and progenitor cell populations. GLIS1, in conjunction with several other reprogramming factors, was shown to markedly increase the efficiency of generating induced pluripotent stem cells (iPSC) from somatic cells. GLIS2 has been reported to contribute to the maintenance of the pluripotent state in hPSCs. In addition, GLIS2 has a function in regulating self-renewal of hematopoietic progenitors and megakaryocytic differentiation...
2017: Stem Cell Investigation
https://www.readbyqxmd.com/read/29046703/dose-dependent-effects-of-busulfan-on-dog-testes-in-preparation-for-spermatogonial-stem-cell-transplantation
#10
Tai-Young Hur, Seung-Hoon Lee, Sun-A Ock, Hyuk Song, Hyun-Jung Park, Ran Lee, Si-Heung Sung, Hyunjhung Jhun, Won-Young Lee
Successful male germ cell transplantation requires depletion of the host germ cells to allow efficient colonization of the donor spermatogonial stem cells. Although a sterilizing drug, busulfan, is commonly used for the preparation of recipient models before transplantation, the optimal dose of this drug has not yet been defined in dogs. In this study, 1-year-old mongrel dogs were intravenously injected with three different concentrations of busulfan (10, 15, or 17.5 mg/kg). Four weeks after busulfan treatment, no fully matured spermatozoa were detected in any of the busulfan-treated groups...
September 2017: Laboratory Animal Research
https://www.readbyqxmd.com/read/29041987/the-effects-of-melatonin-on-colonization-of-neonate-spermatogonial-mouse-stem-cells-in-a-three-dimensional-soft-agar-culture-system
#11
Shadan Navid, Mehdi Abbasi, Yumi Hoshino
BACKGROUND: Melatonin is a pleiotropic hormone with powerful antioxidant activity both in vivo and in vitro. The present study aimed to investigate the effects of melatonin on the proliferation efficiency of neonatal mouse spermatogonial stem cells (SSCs) using a three-dimensional soft agar culture system (SACS) which has the capacity to induce development of SSCs similar to in vivo conditions. METHODS: SSCs were isolated from testes of neonate mice and their purities were assessed by flow cytometry using PLZF antibody...
October 17, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29040674/evaluation-of-apoptotic-and-autophagic-related-protein-expressions-before-and-after-ivm-of-fresh-slow-frozen-and-vitrified-pre-pubertal-mouse-testicular-tissue
#12
L Dumont, F Chalmel, A Oblette, B Berby, A Rives, V Duchesne, C Rondanino, N Rives
STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: IVM strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i...
November 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29037932/retinoic-acid-deficiency-leads-to-an-increase-in-spermatogonial-stem-number-in-the-neonatal-mouse-testis-but-excess-retinoic-acid-results-in-no-change
#13
Kellie S Agrimson, Melissa J Oatley, Debra Mitchell, Jon M Oatley, Michael D Griswold, Cathryn A Hogarth
The onset of spermatogenesis occurs in response to retinoic acid (RA), the active metabolite of vitamin A. However, whether RA plays any role during establishment of the spermatogonial stem cell (SSC) pool is unknown. Because designation of the SSC population and the onset of RA signaling in the testis that induces differentiation have similar timing, this study asked whether RA influenced SSC establishment. Whole mount immunofluorescence and flow cytometric analysis using the Id4-eGfp transgenic reporter mouse line revealed an enrichment for ID4-EGFP+ cells within the testis following inhibition of RA synthesis by WIN 18,446 treatment...
October 14, 2017: Developmental Biology
https://www.readbyqxmd.com/read/29022902/differential-regulation-of-spermatogenic-process-by-lkb1-isoforms-in-mouse-testis
#14
Feifei Kong, Mei Wang, Xiaojing Huang, Qiuling Yue, Xiang Wei, Xiaowei Dou, Xiaoxu Peng, Yuanyuan Jia, Ke Zheng, Tinghe Wu, Jun Yan, Jing Li
Liver serine/threonine kinase B1 (LKB1) is a tumor suppressor associated with the pathogenesis of Peutz-Jeghers syndrome. Affected males are at increased risk of developing Sertoli cell tumors and display defective spermatogenesis. Male mice lacking the short isoform (Lkb1S) of Lkb1 were sterile and exhibited abnormal spermiogenesis. In addition to the short isoform, the long isoform of Lkb1 (Lkb1L) is also expressed in testis; however, the requirement of the long isoform for fertility and the functional difference between the isoforms remain unknown...
October 12, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28992003/rearing-in-captivity-affects-spermatogenesis-and-sperm-quality-in-greater-amberjack-seriola-dumerili-risso-1810
#15
R Zupa, C Fauvel, C C Mylonas, C Pousis, N Santamaria, Μ Papadaki, I Fakriadis, V Cicirelli, S Mangano, L Passantino, G M Lacalandra, Aldo Corriero
The greater amberjack, (Risso, 1810), is a promising candidate for the diversification of European aquaculture production, but inconsistent reproduction in captivity prevents commercial production. Recent studies showed that greater amberjack confined in sea cages exhibited scarce gonad development and early interruption of gametogenic activity during the reproductive season. The aim of the present study was to improve our understanding of the observed impairment of spermatogenesis. Adult wild and captive-reared males were sampled during 3 different phases of the reproductive cycle: early gametogenesis (EARLY; late April to early May), advanced gametogenesis (ADVANCED; late May to early June), and spawning (SPAWNING; late June to July)...
September 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28985528/chromatin-and-single-cell-rna-seq-profiling-reveal-dynamic-signaling-and-metabolic-transitions-during-human-spermatogonial-stem-cell-development
#16
Jingtao Guo, Edward J Grow, Chongil Yi, Hana Mlcochova, Geoffrey J Maher, Cecilia Lindskog, Patrick J Murphy, Candice L Wike, Douglas T Carrell, Anne Goriely, James M Hotaling, Bradley R Cairns
Human adult spermatogonial stem cells (hSSCs) must balance self-renewal and differentiation. To understand how this is achieved, we profiled DNA methylation and open chromatin (ATAC-seq) in SSEA4(+) hSSCs, analyzed bulk and single-cell RNA transcriptomes (RNA-seq) in SSEA4(+) hSSCs and differentiating c-KIT(+) spermatogonia, and performed validation studies via immunofluorescence. First, DNA hypomethylation at embryonic developmental genes supports their epigenetic "poising" in hSSCs for future/embryonic expression, while core pluripotency genes (OCT4 and NANOG) were transcriptionally and epigenetically repressed...
October 5, 2017: Cell Stem Cell
https://www.readbyqxmd.com/read/28982932/ezh2-deletion-promotes-spermatogonial-differentiation-and-apoptosis
#17
Cheng Jin, Yan Zhang, Zhi-Peng Wang, Xiu-Xia Wang, Tie-Cheng Sun, Xiao-Yu Li, Ji-Xin Tang, Jin-Mei Cheng, Jian Li, Su-Ren Chen, Shou-Long Deng, Yi-Xun Liu
Spermatogenesis is crucial for male fertility and is therefore tightly controlled by a variety of epigenetic regulators. However, the function of enhancer of zeste homolog 2 (EZH2) in spermatogenesis and the molecular mechanisms underlying its activity remain poorly defined. Here, we demonstrate that deleting EZH2 promoted spermatogonial differentiation and apoptosis. EZH2 is expressed in spermatogonia, spermatocytes and round and elongated spermatids from stage 9 to 11 but not in leptotene and zygotene spermatocytes...
November 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28982519/nanos2-is-a-molecular-marker-of-inchoate-buffalo-spermatogonia
#18
Meng-Qi Li, Ao-Lin Luo, Peng-Wei Zhao, Ting-Ting Li, Shuang-Shuang Geng, Xing-Wei Liang, Hui-Yan Xu, Yang-Qing Lu, Sheng-Sheng Lu, Xiao-Gan Yang, Ke-Huan Lu
Nanos2 belongs to the Nanos gene-coding family and is an important RNA-binding protein that has been shown to have essential roles in male germline stem cells development and self-renewal in mouse. However, little is known about Nanos2 in inchoate buffalo spermatogonia. Here, rapid-amplification of cDNA ends (RACE) was used to obtain the full-length buffalo Nanos2 sequence and bioinformatic analysis revealed a highly conserved Nanos2 sequence between buffalo and other mammalian species. Although Nanos2 was expressed in various tissues, the highest mRNA expression levels were found in testes tissue...
November 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28950662/the-antineoplastic-busulphan-impairs-peritubular-and-leydig-cells-and-vitamin-b12-stimulates-spermatogonia-proliferation-and-prevents-busulphan-induced-germ-cell-death
#19
Estela Sasso-Cerri, Bárbara Oliveira, Fabiane de Santi, Flávia L Beltrame, Breno H Caneguim, Paulo S Cerri
Busulphan (Bu), an alkylating agent used for bone marrow and spermatogonial stem cell transplantation (SSCT), impairs Sertoli (SC) cells, which are necessary for the spermatogonial stem cell (SSC) homing during transplantation. As Leydig (LC) and peritubular myoid (PMC) cells are essential for SC support and maintenance of spermatogonial niche, we evaluated the impact of Bu on the LC and PMC structural integrity. Vitamin B12 (B12) has demonstrated beneficial effects against drug-induced testicular changes; thus, we also examined whether this vitamin is able to stimulate spermatogonia mitotic activity and prevent Bu-induced germ cell death...
November 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28950090/spermatogenesis
#20
Hitoshi Nishimura, Steven W L'Hernault
Most organisms consist of two cell lineages - somatic cells and germ cells. The former are required for the current generation, and the latter create offspring. Male and female germ cells are usually produced during spermatogenesis and oogenesis, which take place in the testis and the ovary, respectively. Spermatogenesis involves the differentiation of spermatogonial stem cells into spermatocytes via mitotic cell division and the production of haploid spermatids from the tetraploid primary spermatocytes via meiotic cell division...
September 25, 2017: Current Biology: CB
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