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MicroRNA 10b

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https://www.readbyqxmd.com/read/28074869/urinary-exosome-derived-micrornas-reflecting-the-changes-of-renal-function-and-histopathology-in-dogs
#1
Osamu Ichii, Hiroshi Ohta, Taro Horino, Teppei Nakamura, Marina Hosotani, Tatsuya Mizoguchi, Keitaro Morishita, Kensuke Nakamura, Yuki Hoshino, Satoshi Takagi, Noboru Sasaki, Mitsuyoshi Takiguchi, Ryo Sato, Kazuhisa Oyamada, Yasuhiro Kon
MicroRNAs act as post-transcriptional regulators, and urinary exosome (UExo)-derived microRNAs may be used as biomarkers. Herein, we screened for UExo-derived microRNAs reflecting kidney disease (KD) status in dogs. Examined dogs were divided into healthy kidney control (HC) and KD groups according to renal dysfunction. We confirmed the appearance of UExo having irregular globe-shapes in a dog by immunoblot detection of the exosome markers, TSG101 and CD9. Based on our previous data using KD model mice and the data obtained herein by next generation sequencing of UExo-derived microRNAs in dogs, miR-26a, miR-146a, miR-486, miR-21a, and miR-10a/b were selected as candidate microRNAs...
January 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28055956/circulating-exosomal-micrornas-as-prognostic-biomarkers-for-non-small-cell-lung-cancer
#2
Qingyun Liu, Zubin Yu, Shuai Yuan, Weijia Xie, Chengying Li, Zeyao Hu, Ying Xiang, Na Wu, Long Wu, Li Bai, Yafei Li
Exosomal miRNAs are proposed as excellent candidate biomarkers for clinical applications. However, little is known about their potential roles as prognostic biomarkers in lung cancer. In this study, we explored the prognostic value of plasma exosomal microRNAs (miRNAs) for non-small-cell lung cancer (NSCLC). Using a quantitative polymerase chain reaction (qPCR) array panel, we analyzed 84 plasma exosomal miRNAs in 10 lung adenocarcinoma patients and 10 matched healthy controls. The qPCR array showed 30 aberrantly expressed exosomal miRNAs...
December 30, 2016: Oncotarget
https://www.readbyqxmd.com/read/28039186/mir-10b-5p-is-a-novel-th17-regulator-present-in-th17-cells-from-ankylosing-spondylitis
#3
L Chen, M H Al-Mossawi, A Ridley, T Sekine, A Hammitzsch, J de Wit, D Simone, H Shi, F Penkava, M Kurowsha-Stolarska, I Pulyakhina, J C Knight, T J Kim, P Bowness
OBJECTIVE: To determine the microRNA (miR) signature in ankylosing spondylitis (AS) T helper (Th)17 cells. METHODS: Interleukin (IL)-17A-producing CD4+ T cells from patients with AS and healthy controls were FACS-sorted for miR sequencing and qPCR validation. miR-10b function was determined by miR mimic expression followed by cytokine measurement, transcriptome analysis, qPCR and luciferase assays. RESULTS: AS Th17 cells exhibited a miR signature characterised by upregulation of miR-155-5p, miR-210-3p and miR-10b...
December 30, 2016: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/27935974/epigallocatechin-3-gallate-upregulates-mir-221-to-inhibit-osteopontin-dependent-hepatic-fibrosis
#4
M L Arffa, M A Zapf, A N Kothari, V Chang, G N Gupta, X Ding, M M Al-Gayyar, W Syn, N M Elsherbiny, P C Kuo, Z Mi
Osteopontin (OPN) promotes hepatic fibrosis, and developing therapies targeting OPN expression in settings of hepatic injury holds promise. The polyphenol epigallocatechin-3-gallate (EGCG), found in high concentrations in green tea, downregulates OPN expression through OPN mRNA degradation, but the mechanism is unknown. Previous work has shown that microRNAs can decrease OPN mRNA levels, and other studies have shown that EGCG modulates the expression of multiple microRNAs. In our study, we first demonstrated that OPN induces hepatic stellate cells to transform into an activated state...
2016: PloS One
https://www.readbyqxmd.com/read/27837403/interplay-of-transcription-factors-and-micrornas-during-embryonic-hematopoiesis
#5
Xueping Gong, Ruihua Chao, Pengxiang Wang, Xiaoli Huang, Jingjing Zhang, Xiaozhou Zhu, Yanyang Zhang, Xue Yang, Chao Hou, Xiangjun Ji, Tieliu Shi, Yuan Wang
Hematopoietic stem cells (HSCs), which are localized in the bone marrow of adult mammals, come from hematopoietic endothelium during embryonic stages. Although the basic processes of HSC generation and differentiation have been described in the past, the epigenetic regulation of embryonic hematopoiesis remains to be fully described. Here, by utilizing an in vitro differentiation system of mouse embryonic stem cells (ESCs), we identified more than 20 microRNAs that were highly enriched in embryonic hematopoietic cells, including some (e...
November 9, 2016: Science China. Life Sciences
https://www.readbyqxmd.com/read/27834869/micrornas-10a-and-10b-regulate-the-expression-of-human-platelet-glycoprotein-ib%C3%AE-for-normal-megakaryopoiesis
#6
Zuping Zhang, Yali Ran, Tanner S Shaw, Yuandong Peng
MicroRNAs are a class of small non-coding RNAs that bind to the three prime untranslated region (3'-UTR) of target mRNAs. They cause a cleavage or an inhibition of the translation of target mRNAs, thus regulating gene expression. Here, we employed three prediction tools to search for potential miRNA target sites in the 3'-UTR of the human platelet glycoprotein (GP) 1BA gene. A luciferase reporter assay shows that miR-10a and -10b sites are functional. When miR-10a or -10b mimics were transfected into the GP Ibβ/GP IX-expressing cells, along with a DNA construct harboring both the coding and 3'-UTR sequences of the human GP1BA gene, we found that they inhibit the transient expression of GP Ibα on the cell surface...
November 9, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27756776/six-serum-based-mirnas-as-potential-diagnostic-biomarkers-for-gastric-cancer
#7
Zebo Huang, Danxia Zhu, Lirong Wu, Mingfeng He, Xin Zhou, Lan Zhang, Huo Zhang, Weiwei Wang, Jun Zhu, Wenfang Cheng, Yan Chen, Yong Fan, Lianwen Qi, Yin Yin, Wei Zhu, Yongqian Shu, Ping Liu
BACKGROUND: Circulating microRNAs (miRNAs) in serum may serve as promising diagnostic biomarkers for patients with gastric cancer (GC). METHODS: Using quantitative reverse transcription polymerase chain reaction (qRT-PCR) based Exiqon panel, we identified 58 differentially expressed miRNAs from 3 GC pool samples and 1 normal control (NC) pool in the initial screening phase. Identified miRNAs were further validated in the training (49 GC VS. 47 NCs) and validation phases (154 GC VS...
October 18, 2016: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/27756250/mir-10b-exerts-oncogenic-activity-in-human-hepatocellular-carcinoma-cells-by-targeting-expression-of-cub-and-sushi-multiple-domains-1-csmd1
#8
Qiao Zhu, Li Gong, Jun Wang, Qian Tu, Li Yao, Jia-Rui Zhang, Xiu-Juan Han, Shao-Jun Zhu, Shu-Mei Wang, Yan-Hong Li, Wei Zhang
BACKGROUND: Hepatocellular carcinoma (HCC) is a lethal disease, while the precise underlying molecular mechanisms of HCC pathogenesis remain to be defined. MicroRNA (miRNA), a class of non-coding small RNAs, can post-transcriptionally regulate gene expression. Altered miRNA expression has been reported in HCCs. This study assessed expression and the oncogenic activity of miRNA-10b (miR-10b) in HCC. METHODS: Forty-five paired human HCC and adjacent non-tumor tissues were collected for qRT-PCR and immunohistochemistry analysis of miR-10b and CUB and Sushi multiple domains 1 (CSMD1), respectively...
October 18, 2016: BMC Cancer
https://www.readbyqxmd.com/read/27682205/impact-of-endurance-exercise-training-on-adipocyte-microrna-expression-in-overweight-men
#9
Thomas Tsiloulis, Joshua Pike, David Powell, Fernando J Rossello, Benedict J Canny, Ruth C R Meex, Matthew J Watt
Adipocytes are major regulators of metabolism, and endurance exercise training improves adipocyte function; however, the molecular mechanisms that regulate chronic adaptive responses remain unresolved. microRNAs (miRNAs) influence adipocyte differentiation and metabolism. Accordingly, we aimed to determine whether adipocyte miRNA expression is responsive to exercise training and to identify exercise-responsive miRNAs that influence adipocyte metabolism. Next-generation sequencing was used to profile miRNA expression of adipocytes that were isolated from abdominal subcutaneous (ABD) and gluteofemoral (GF) adipose tissue of overweight men before and after 6 wk of endurance exercise training...
January 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27624142/potential-role-of-microrna-10b-down-regulation-in-cardiomyocyte-apoptosis-in-aortic-stenosis-patients
#10
Idoia Gallego, Javier Beaumont, Begoña López, Susana Ravassa, Juan José José Gómez-Doblas, María Ujué Ujué Moreno, Félix Valencia, Eduardo de Teresa, Javier Díez, Arantxa González
MicroRNAs have been associated with cardiomyocyte apoptosis, a process involved in myocardial remodelling in aortic valve (Av) stenosis (AS). Our aim was to analyse whether the dysregulation of myocardial microRNAs was related to cardiomyocyte apoptosis in AS patients. Endomyocardial biopsies were obtained from 28 patients with severe AS (based on pressure gradients and Av area) referred to Av replacement and from necropsies of 10 cardiovascular disease-free control subjects. AS patients showed an increased ( P <0...
September 13, 2016: Clinical Science (1979-)
https://www.readbyqxmd.com/read/27623773/profiling-bovine-blastocyst-micrornas-using-deep-sequencing
#11
R Pasquariello, B Fernandez-Fuertes, F Strozzi, F Pizzi, R Mazza, P Lonergan, F Gandolfi, J L Williams
MicroRNAs (miRNAs) are known to control several reproductive functions, including oocyte maturation, implantation and early embryonic development. Recent advances in deep sequencing have allowed the analysis of all miRNAs of a sample. However, when working with embryos, due to the low RNA content, miRNA profiling is challenging because of the relatively large amount of total RNA required for library preparation protocols. In the present study we compared three different procedures for RNA extraction and prepared libraries using pools of 30 bovine blastocysts...
September 13, 2016: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/27611973/profile-of-exosomal-and-intracellular-microrna-in-gamma-herpesvirus-infected-lymphoma-cell-lines
#12
Shiho Hoshina, Tsuyoshi Sekizuka, Michiyo Kataoka, Hideki Hasegawa, Hiromichi Hamada, Makoto Kuroda, Harutaka Katano
Exosomes are small vesicles released from cells, into which microRNAs (miRNA) are specifically sorted and accumulated. Two gamma-herpesviruses, Kaposi sarcoma-associated herpesvirus (KSHV) and Epstein-Barr virus (EBV), encode miRNAs in their genomes and express virus-encoded miRNAs in cells and exosomes. However, there is little information about the detailed distribution of virus-encoded miRNAs in cells and exosomes. In this study, we thus identified virus- and host-encoded miRNAs in exosomes released from KSHV- or EBV-infected lymphoma cell lines and compared them with intracellular miRNAs using a next-generation sequencer...
2016: PloS One
https://www.readbyqxmd.com/read/27611783/differences-in-circulating-micrornas-between-grazing-and-grain-fed-wagyu-cattle-are-associated-with-altered-expression-of-intramuscular-microrna-the-potential-target-pten-and-lipogenic-genes
#13
Susumu Muroya, Masahiro Shibata, Masayuki Hayashi, Mika Oe, Koichi Ojima
We aimed to understand the roles of miRNAs in the muscle tissue maturation and those of circulating microRNAs (c-miRNAs) in beef production of Japanese Black (JB) cattle (Wagyu), a breed with genetically background of superior intermuscular fat depot, by comparing different feeding conditions (indoor grain-feeding vs. grazing on pasture). The cattle at 18 months old were assigned to pasture feeding or conventional indoor grain feeding conditions for 5 months. Microarray analysis of c-miRNAs from the plasma extracellular vesicles led to the detection of a total of 202 bovine miRNAs in the plasma, including 15 miRNAs that differed between the feeding conditions...
2016: PloS One
https://www.readbyqxmd.com/read/27592860/up-regulation-of-mir-10b-predicate-advanced-clinicopathological-features-and-liver-metastasis-in-colorectal-cancer
#14
Hong Jiang, Jijun Liu, Yingtao Chen, Chong Ma, Baosong Li, Tao Hao
Given the emerging role of microRNA in tumor disease progression, we investigated the association between miRNA 10b expression, liver metastasis, and clinicopathological of colorectal cancer (CRC). Two hundred and forty-six pairs of samples (including CRC samples and normal adjacent tissues) from CRC patients were collected from May 2004 to May 2009. All samples verified to contain at least 80% tumor cells, and were immediately frozen in liquid nitrogen and stored at -80°C or fixed in 10% formalin for paraffin embedding...
September 4, 2016: Cancer Medicine
https://www.readbyqxmd.com/read/27569213/ablation-of-mir-10b-suppresses-oncogene-induced-mammary-tumorigenesis-and-metastasis-and-reactivates-tumor-suppressive-pathways
#15
Jongchan Kim, Ashley N Siverly, Dahu Chen, Min Wang, Yuan Yuan, Yumeng Wang, Hyemin Lee, Jinsong Zhang, William J Muller, Han Liang, Boyi Gan, Xianbin Yang, Yutong Sun, M James You, Li Ma
The invasive and metastatic properties of many human tumors have been associated with upregulation of the miRNA miR-10b, but its functional contributions in this setting have not been fully unraveled. Here, we report the generation of miR-10b-deficient mice, in which miR-10b is shown to be largely dispensable for normal development but critical to tumorigenesis. Loss of miR-10b delays oncogene-induced mammary tumorigenesis and suppresses epithelial-mesenchymal transition, intravasation, and metastasis in a mouse model of metastatic breast cancer...
November 1, 2016: Cancer Research
https://www.readbyqxmd.com/read/27565813/expression-of-the-circulating-and-the-tissue-micrornas-after-surgery-chemotherapy-and-radiotherapy-in-mice-mammary-tumor
#16
Sadaf Farsinejad, Mahdi Rahaie, Ali Mohammad Alizadeh, Mohammad Mir-Derikvand, Zohre Gheisary, Hassan Nosrati, Solmaz Khalighfard
The expression of microRNAs (miRNAs), as novel biomarkers, is subject to change in many cancers. Therefore, the overall profile of miRNAs can be used for detection of cancer type, response to therapies, pathological variables, and other factors related to the disease. In this study, to evaluate miRNA expression associated with the tumor progression and response to treatment, 60 BALB/c mice received subcutaneous injections of 4T1 cells. The study includes ten groups: one group as control, six groups were euthanized at different time points to assess the role of miRNA expression in the tumor progression, and three groups received chemotherapy, radiotherapy, and surgery to evaluate miRNA expression in response to treatment...
October 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27557737/high-throughput-mirna-sequencing-and-identification-of-biomarkers-for-forensically-relevant-biological-fluids
#17
Sarah Seashols-Williams, Carolyn Lewis, Chelsea Calloway, Nerissa Peace, Ariana Harrison, Christina Hayes-Nash, Samantha Fleming, Qianni Wu, Zendra E Zehner
microRNAs (miRNAs) are small noncoding RNAs that regulate cellular processes through modulation of proteins at the translational level. They tend to be highly stable as compared to other RNA species due to their small size and protection by protein and/or lipid matrices. Thus, it is likely that miRNAs, when fully evaluated, will make excellent candidates for body fluid identification. miRNA analysis of body fluids has been the subject of some recent interest in the forensic community. In this study, small RNAs were isolated from individual donations of eight forensically relevant biological fluids (blood, semen, vaginal fluid, menstrual blood, saliva, urine, feces, and perspiration) and subjected to next generation sequencing using the Illumina Hi-Seq platform...
October 2016: Electrophoresis
https://www.readbyqxmd.com/read/27538692/circulating-micrornas-as-potential-diagnostic-biomarkers-and-therapeutic-targets-in-gastric-cancer-current-status-and-future-perspectives
#18
Mahla Arabpour, Saeid Mohammadparast, Mina Maftouh, Soudabeh ShahidSales, Soheila Moieni, Hanie Akbarzade, Seyed Reza Mirhafez, Seyed Mohammd Reza Parizadeh, Seyed Mahdi Hassanian, Amir Avan
Gastric cancer is among the leading cause of cancer related death worldwide. Patients with gastric cancer are typically asymptomatic, and diagnosed at late stages, supporting the need for identification of novel prognostic and diagnostic biomarkers. Recently, microRNAs have emerged as molecular regulators that can have key roles in pathogenesis and progression of different malignancies, including gastric cancer. There is growing body of evidence showing the aberrant activation of some known circulating miRNAs, e...
August 17, 2016: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/27508339/tailored-nanoparticle-codelivery-of-antimir-21-and-antimir-10b-augments-glioblastoma-cell-kill-by-temozolomide-toward-a-personalized-anti-microrna-therapy
#19
Jeyarama S Ananta, Ramasamy Paulmurugan, Tarik F Massoud
Glioblastoma remains an aggressive brain malignancy with poor prognosis despite advances in multimodal therapy that include standard use of Temozolomide. MicroRNA-21 (miR-21) and microRNA-10b (miR-10b) are oncomiRs overexpressed in glioblastoma, promoting many aspects of cancer biology. We hypothesized that PLGA nanoparticles carrying antisense miR-21 (antimiR-21) and antisense miR-10b (antimiR-10b) might beneficially knockdown endogenous miR-21 and miR-10b function and reprogram cells prior to Temozolomide treatment...
September 6, 2016: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/27494896/microrna-10b-promotes-abnormal-expression-of-the-proto-oncogene-c-jun-in-metastatic-breast-cancer-cells
#20
Revital Knirsh, Iris Ben-Dror, Shira Modai, Noam Shomron, Lily Vardimon
MicroRNAs have been shown to act as oncogenes or tumor suppressers via various cellular pathways. Specifically, in breast cancer, upregulation of miR-10b is positively associated with aggressiveness of tumors. However, the mechanism by which miR-10b contributes to cell malignancy is largely unknown. Here we show that at the receiving end of the miR-10b pathway is the proto-oncogene c-Jun, a transcription factor that plays a critical role in stimulation of cell proliferation and tumor progression. c-Jun is known to be translationally activated by loss of cell contacts or restructuring of the cytoskeleton...
September 13, 2016: Oncotarget
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