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https://www.readbyqxmd.com/read/28098806/antibacterial-activities-and-possible-modes-of-action-of-acacia-nilotica-l-del-against-multidrug-resistant-escherichia-coli-and-salmonella
#1
Muhammad Bilal Sadiq, Joel Tarning, Tay Zar Aye Cho, Anil Kumar Anal
Medicinal plants are frequently used for the treatment of various infectious diseases. The objective of this study was to evaluate the antibacterial activity and mode of action of Acacia nilotica and the antibiogram patterns of foodborne and clinical strains of Escherichia coli and Salmonella. The mechanism of action of acacia extracts against E. coli and Salmonella was elucidated by observing morphological damages including cell integrity and cell membrane permeability, as well as changes in cell structures and growth patterns in kill-time experiments...
January 14, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28098803/optimization-of-the-production-of-1-phenylethanol-using-enzymes-from-flowers-of-tea-camellia-sinensis-plants
#2
Fang Dong, Ying Zhou, Lanting Zeng, Naoharu Watanabe, Xinguo Su, Ziyin Yang
1-Phenylethanol (1PE) can be used as a fragrance in food flavoring and cosmetic industries and as an intermediate in the pharmaceutical industry. 1PE can be synthesized from acetophenone, and the cost of 1PE is higher than the cost of acetophenone. Therefore, it is important to establish an effective and low-cost approach for producing 1PE. Our previous studies found that tea (Camellia sinensis) flowers, which are an abundant and waste resource, contained enzymes that could transform acetophenone to 1PE. In the present study, we extracted crude enzymes from tea flowers and optimized the production conditions of 1PE using response surface methodology...
January 13, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28098796/comparative-metabolite-profiling-of-triterpenoid-saponins-and-flavonoids-in-flower-color-mutations-of-primula-veris-l
#3
Lysanne Apel, Dietmar R Kammerer, Florian C Stintzing, Otmar Spring
Primula veris L. is an important medicinal plant with documented use for the treatment of gout, headache and migraine reaching back to the Middle Ages. Triterpenoid saponins from roots and flowers are used in up-to-date phytotherapeutic treatment of bronchitis and colds due to their expectorant and secretolytic effects. In addition to the wild type plants with yellow petals, a red variant and an intermediate orange form of Primula veris L. have recently been found in a natural habitat. The secondary metabolite profiles of roots, leaves and flowers of these rare variants were investigated and compared with the wild type metabolome...
January 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28098142/carbon-translocation-from-a-plant-to-an-insect-pathogenic-endophytic-fungus
#4
Scott W Behie, Camila C Moreira, Irina Sementchoukova, Larissa Barelli, Paul M Zelisko, Michael J Bidochka
Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen. Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia. We speculated that in exchange for this insect-derived nitrogen, the plant would provide photosynthate to the fungus. By using (13)CO2, we show the incorporation of (13)C into photosynthate and the subsequent translocation of (13)C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex...
January 18, 2017: Nature Communications
https://www.readbyqxmd.com/read/28097599/biosynthesis-characterization-and-bioactivities-evaluation-of-silver-and-gold-nanoparticles-mediated-by-the-roots-of-chinese-herbal-angelica-pubescens-maxim
#5
Josua Markus, Dandan Wang, Yeon-Ju Kim, Sungeun Ahn, Ramya Mathiyalagan, Chao Wang, Deok Chun Yang
A facile synthesis and biological applications of silver (DH-AgNps) and gold nanoparticles (DH-AuNps) mediated by the aqueous extract of Angelicae Pubescentis Radix (Du Huo) are explored. Du Huo is a medicinal root belonging to Angelica pubescens Maxim which possesses anti-inflammatory, analgesic, and antioxidant properties. The absorption spectra of nanoparticles in varying root extract and metal ion concentration, pH, reaction temperatures, and time were recorded by ultraviolet-visible (UV-Vis) spectroscopy...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28097405/role-of-exopolysaccharide-in-salt-stress-resistance-and-cell-motility-of-mesorhizobium-alhagi-ccnwxj12-2-t
#6
Xiaodong Liu, Yantao Luo, Zhefei Li, Jiamei Wang, Gehong Wei
Mesorhizobium alhagi, a legume-symbiont soil bacterium that forms nodules with the desert plant Alhagi sparsifolia, can produce large amounts of exopolysaccharide (EPS) using mannitol as carbon source. However, the role of EPS in M. alhagi CCNWXJ12-2(T), an EPS-producing rhizobium with high salt resistance, remains uncharacterized. Here, we studied the role of EPS in M. alhagi CCNWXJ12-2(T) using EPS-deficient mutants constructed by transposon mutagenesis. The insertion sites of six EPS-deficient mutants were analyzed using single primer PCR, and two putative gene clusters were found to be involved in EPS synthesis...
January 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28096967/volatile-compounds-analysis-and-antioxidant-antimicrobial-and-cytotoxic-activities-of-mindium-laevigatum
#7
Abdolrasoul Haghir Ebrahimabadi, Mohammad Mahdi Movahedpour, Hossain Batooli, Ebrahim Haghir Ebrahimabadi, Asma Mazoochi, Maryam Mobarak Qamsari
OBJECTIVES: Mindium laevigatum is an endemic plant of Iran and Turkey and is widely used as blood purifier, antiasthma and antidyspnea in traditional medicine. Chemical composition of volatile materials of the plant and its antioxidant, antimicrobial and cytotoxic activities were reported in this study. MATERIALS AND METHODS: Simultaneous distillation-extraction (SDE) and GC-Mass-FID analysis were used for the plant volatile materials chemical composition identification and quantification...
December 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28095835/antimicrobial-activity-of-some-plant-materials-used-in-armenian-traditional-medicine
#8
Mikayel Ginovyan, Margarit Petrosyan, Armen Trchounian
BACKGROUND: Antibiotic resistance has become one of the major problems facing humanity. The need for new antimicrobials has been increased dramatically. Plants are considered as one of the most promising sources for new antimicrobials discovery. Despite relatively small area, Armenia has large diversity of flora with many endemic species. In Armenian folk medicine plant materials have been used to treat various microbial diseases since ancient times. The goal of our research was to evaluate antimicrobial efficiency of different parts of five wild plants species which are commonly used in Armenian traditional medicine...
January 17, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28095717/functional-constituents-of-wild-and-cultivated-goji-l-barbarum-l-leaves-phytochemical-characterization-biological-profile-and-computational-studies
#9
Andrei Mocan, Gökhan Zengin, Mario Simirgiotis, Michaela Schafberg, Adriano Mollica, Dan C Vodnar, Gianina Crişan, Sascha Rohn
Goji (Lycium barbarum L.) leaves are emphasized as a functional tea or as dietary supplements. The phenolic compound profile, antioxidant, enzyme inhibitory, antimicrobial, and antimutagenic activities of leaf extracts from two selected cultivars in comparison with wild-growing plants have been evaluated. HPLC-DAD/ESI-ToF-MS analysis revealed the presence of phenolic acids and flavonoids with chlorogenic acid and rutin being the dominant compounds in the cultivated plants, whereas rutin and kaempeferol-3-O-rutinoside for wild growing ones...
December 2017: Journal of Enzyme Inhibition and Medicinal Chemistry
https://www.readbyqxmd.com/read/28095587/quantitative-determination-of-lactones-in-piper-methysticum-kava-kava-by-supercritical-fluid-chromatography
#10
Adele Murauer, Markus Ganzera
A fast and validated supercritical fluid chromatography method for the quantitative determination of major lactones in Piper methysticum, a plant used against nervous anxiety, stress, and restlessness, was developed. The baseline separation of dihydrokavain, demethoxyyangonin, kavain, yangonin, dihydromethysticin, and methysticin was possible in less than 4 min on an Aquity UPC(2) BEH 1.7 µm column, in combination with a mobile phase comprising CO2 and methanol with diethylamine. The column temperature had a great impact on the results because only at 70 °C could kavain and yangonin be fully resolved...
January 17, 2017: Planta Medica
https://www.readbyqxmd.com/read/28094896/characterisation-of-glufosinate-resistance-mechanisms-in-eleusine-indica
#11
Adam Jalaludin, Qin Yu, Peter Zoellner, Roland Beffa, Stephen B Powles
BACKGROUND: An Eleusine indica population has evolved resistance to glufosinate, a major post-emergence herbicide of global agriculture. This population was analysed for target-site (glutamine synthetase) and non-target-site (glufosinate uptake, translocation and metabolism) resistance mechanisms. RESULTS: Glutamine synthetase (GS) activity extracted from susceptible (S) and resistant (R*) plants was equally sensitive to glufosinate inhibition, with IC50 values of 0...
January 17, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28094544/red-wine-and-pomegranate-extracts-suppress-cured-meat-promotion-of-colonic-mucin-depleted-foci-in-carcinogen-induced-rats
#12
Nadia M Bastide, Nathalie Naud, Gilles Nassy, Jean-Luc Vendeuvre, Sylviane Taché, Françoise Guéraud, Ditte A Hobbs, Gunter G Kuhnle, Denis E Corpet, Fabrice H F Pierre
Processed meat intake is carcinogenic to humans. We have shown that intake of a workshop-made cured meat with erythorbate promotes colon carcinogenesis in rats. We speculated that polyphenols could inhibit this effect by limitation of endogenous lipid peroxidation and nitrosation. Polyphenol-rich plant extracts were added to the workshop-made cured meat and given for 14 days to rats and 100 days to azoxymethane-induced rats to evaluate the inhibition of preneoplastic lesions. Colons of 100-d study were scored for precancerous lesions (mucin-depleted foci, MDF), and biochemical end points of peroxidation and nitrosation were measured in urinary and fecal samples...
January 17, 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28094495/bacoside-a-an-indian-traditional-medicine-substance-inhibits-beta-amyloid-cytotoxicity-fibrillation-and-membrane-interactions
#13
Ravit Malishev, Shira Shaham-Niv, Sukhendu Nandi, Sofiya Kolusheva, Ehud Gazit, Raz Jelinek
Bacoside-A, a family of compounds extracted from the Bacopa monniera plant, is a folk-medicinal substance believed to exhibit therapeutic properties, particularly enhancing cognitive functions and improving memory. We show that bacoside-A exerted significant inhibitory effects upon cytotoxicity, fibrillation, and particularly membrane interactions of amyloid-beta (1-42) (Aβ42), the peptide playing a prominent role in Alzeheimer's disease progression and toxicity. Specifically, pre-incubation of bacoside-A with Aβ42 significantly reduced cell toxicity and inhibited fibril formation both in buffer solution and, more significantly, in the presence of membrane vesicles...
January 17, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28094064/bioprospecting-of-antimicrobial-activity-of-extracts-of-endophytic-fungi-from-bauhinia-guianensis
#14
Eduardo A A Pinheiro, Jeferson R S Pina, André O Feitosa, Josiwander M Carvalho, Fábio C Borges, Patrícia S B Marinho, Andrey M R Marinho
Antibiotic resistance results in higher medical costs, prolonged hospital stays and increased mortality and is rising to dangerously high levels in all parts of the world. Therefore, this study aims to search for new antimicrobial agents through bioprospecting of extracts of endophytic fungi from Bauhinia guianensis, a typical Amazonian plant used in combating infections. Seventeen (17) fungi were isolated and as result the methanolic extract of the fungus Exserohilum rostratum showed good activity against the bacteria tested...
January 13, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28094000/molecular-tracing-of-the-origin-of-six-different-plant-species-in-bee-honey-using-real-time-pcr
#15
Yajun Wu, Yange Yang, Mingchang Liu, Bin Wang, Meige Li, Ying Chen
The quality of honey is significantly influencedby floral origin. Mislabeling floral species occursfrequently in beehoney products. To protectconsumers from economic fraud and maintain afair market environment, methods to identify floralspecies in honey are necessary. In our study, realtimePCRs were established, targeting six honeytypes mainly produced in China (canola, Chinesemilkvetch, Chinese chaste tree, locust tree, litchi,and longan). Sensitivity testing on DNA from planttissues exhibited LODs of about 0...
January 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28093936/biological-activities-of-rosmarinus-officinalis-l-rosemary-extract-as-analyzed-in-microorganisms-and-cells
#16
Jonatas Rafael de Oliveira, Daiane de Jesus, Leandro Wagner Figueira, Felipe Eduardo de Oliveira, Cristina Pacheco Soares, Samira Estves Afonso Camargo, Antonio Olavo Cardoso Jorge, Luciane Dias de Oliveira
R. officinalis L. is an aromatic plant commonly used as condiment and for medicinal purposes. Biological activities of its extract were evaluated in this study, as antimicrobial effect on mono- and polymicrobial biofilms, cytotoxicity, anti-inflammatory capacity, and genotoxicity. Monomicrobial biofilms of Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and Pseudomonas aeruginosa and polymicrobial biofilms composed of C. albicans with each bacterium were formed in microplates during 48 h and exposed for 5 min to R...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28093319/anti-inflammatory-effect-of-the-salvia-sclarea-l-ethanolic-extract-on-lipopolysaccharide-induced-periodontitis-in-rats
#17
Milica Kostić, Dušanka Kitić, Milica B Petrović, Tatjana Jevtović-Stojmenov, Marko Jović, Aleksandar Petrović, Slavoljub Živanović
ETHNOPHARMACOLOGICAL RELEVANCE: Salvia sclarea L., clary, is an aromatic plant traditionally used in folk medicine for the treatment of various diseases and conditions. Although it has been primarily used as a stomachic, there are data on traditional use of S. sclarea as an agent against gingivitis, stomatitis and aphthae. AIM OF THE STUDY: The aim of the study was to examine the effect of the S. sclarea ethanolic extract on the lipopolysaccharide (LPS)-induced periodontitis in rats from the immunological and histopathological standpoint...
January 13, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28093019/prophylactic-and-curative-effect-of-rosemary-leaves-extract-in-a-bleomycin-model-of-pulmonary-fibrosis
#18
Sana Bahri, Ridha Ben Ali, Khaoula Gasmi, Mona Mlika, Saloua Fazaa, Riadh Ksouri, Raja Serairi, Saloua Jameleddine, Vadim Shlyonsky
CONTEXT: Pulmonary fibrosis is a devastating disease without effective treatment. Rosemary is appreciated since ancient times for its medicinal properties, while biomolecules originated from the plant have an antioxidant and antifibrotic effect. OBJECTIVE: The effects of Rosmarinus officinalis L. (Lamiaceae) leaves extract (RO) on bleomycin-induced lung fibrosis were investigated. MATERIALS AND METHODS: Male Wistar rats were given a single dose of bleomycin (BLM, 4 mg/kg, intratracheal), while RO (75 mg/kg, intraperitoneal) was administered 3 days later and continued for 4 weeks (BLM/RO1-curative group)...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28092841/phytochemical-study-and-protective-effect-of-trigonella-foenum-graecum-fenugreek-seeds-against-carbon-tetrachloride-induced-toxicity-in-liver-and-kidney-of-male-rat
#19
Sakhria Mbarki, Hichem Alimi, Hafsia Bouzenna, Abdelfettah Elfeki, Najla Hfaiedh
Liver and kidney diseases are a global concern, therefore considerable efforts to obtain fine herbs useful as drugs from medicinal plants are currently in progress. The aim of this work was to study the antioxidant effects of previous supplementation with fenugreek seeds (FS) against carbon tetrachloride (CCl4) toxicity in the liver and kidney. CCl4 toxicity was induced by one dose (i.g. 5ml CCl4/kg of body weight, 50% CCl4 in olive oil) after 7 weeks of normal diet or diet rich in 10% of grinded fenugreek seeds (20g of pellet rat food/rat/day)...
January 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28092749/public-value-at-risk-from-phytophthora-ramorum-and-phytophthora-kernoviae-spread-in-england-and-wales
#20
Ben Drake, Glyn Jones
Heritage gardens, heathland and woodland are increasingly under threat from the non-native tree and plant diseases Phytophthora ramorum and Phytophthora kernoviae. However, there exist only limited literature that estimates the public non-market value that may be lost from a continued spread of Phytophthora ramorum and Phytophthora kernoviae into these habitats. This paper therefore uses a contingent valuation survey to assess the non-extractive public use and non-use values at risk from an uncontrolled spread of these diseases in England and Wales...
January 13, 2017: Journal of Environmental Management
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