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https://www.readbyqxmd.com/read/29324679/antibacterial-evaluation-and-virtual-screening-of-new-thiazolyl-triazole-schiff-bases-as-potential-dna-gyrase-inhibitors
#1
Cristina Nastasă, Dan C Vodnar, Ioana Ionuţ, Anca Stana, Daniela Benedec, Radu Tamaian, Ovidiu Oniga, Brînduşa Tiperciuc
The global spread of bacterial resistance to drugs used in therapy requires new potent and safe antimicrobial agents. DNA gyrases represent important targets in drug discovery. Schiff bases, thiazole, and triazole derivatives are considered key scaffolds in medicinal chemistry. Fifteen thiazolyl-triazole Schiff bases were evaluated for their antibacterial activity, measuring the growth inhibition zone diameter, the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC), against Gram-positive (Staphylococcus aureus, Listeria monocytogenes) and Gram-negative (Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa) bacteria...
January 11, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29324228/lactateing-salmonella-a-host-derived-fermentation-product-fuels-pathogen-growth
#2
Bärbel Stecher, Kirsten Jung
Infection by Salmonella enterica serovar Typhimurium is accompanied by dysbiosis and a decrease of microbiota-derived butyrate. In this issue of Cell Host & Microbe, Gillis et al. (2018) demonstrate that the lack of butyrate reprograms colonic epithelial metabolism toward lactate fermentation. Lactate is then used as a respiratory electron donor, supporting Salmonella growth and thus promoting infection.
January 10, 2018: Cell Host & Microbe
https://www.readbyqxmd.com/read/29301495/cattle-infection-response-network-and-its-functional-modules
#3
Hamid Beiki, Abbas Pakdel, Ardeshir Nejati Javaremi, Ali Masoudi-Nejad, James M Reecy
BACKGROUND: Weighted Gene Co-expression Network analysis, a powerful technique used to extract co-expressed gene pattern from mRNA expression data, was constructed to infer common immune strategies used by cattle in response to five different bacterial species (Escherichia coli, Mycobacterium avium, Mycobacterium bovis, Salmonella and Staphylococcus aureus) and a protozoa (Trypanosoma Congolense) using 604 publicly available gene expression microarrays from 12 cattle infection experiments...
January 5, 2018: BMC Immunology
https://www.readbyqxmd.com/read/29299944/evaluation-of-genotoxic-potential-of-avarol-avarone-and-its-methoxy-and-methylamino-derivatives-in-prokaryotic-and-eukaryotic-test-models
#4
Stoimir Kolarević, Dragana Milovanović, Margareta Kračun-Kolarević, Jovana Kostić, Karolina Sunjog, Rajko Martinović, Jelena Đorđević, Irena Novaković, Dušan Sladić, Branka Vuković-Gačić
In this study, mutagenic and genotoxic potential of anti-tumor compounds avarol, avarone, and its derivatives 3'-methoxyavarone, 4'-(methylamino)avarone and 3'-(methylamino)avarone was evaluated and compared to cytostatics commonly used in chemotherapy (5-fluorouracil, etoposid, and cisplatin). Mutagenic potential of selected hydroquinone and quinones was assessed in prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002. Genotoxic potential was also assessed in eukaryotic models using comet assay in human fetal lung cell line (MRC-5), human adenocarcinoma epithelial cell line (A549), and in human peripheral blood cells (HPBC)...
January 4, 2018: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/29277660/properties-and-mechanism-of-d-glucosaminate-6-phosphate-ammonia-lyase-an-aminotransferase-family-enzyme-with-d-amino-acid-specificity
#5
Robert S Phillips, Samuel C Ting, Ange G Tetsadjio, Kaitlin L Anderson, Kyle M Friez, Katherine A Miller, Timothy R Hoover
Salmonella enterica serovar Typhimurium utilizes a wide range of growth substrates, some of which are relatively novel. One of these unusual substrates is d-glucosaminate, which is metabolized by the enzymes encoded in the dga operon. d-Glucosaminate is transported and converted to d-glucosaminate-6-phosphate (G6P) by a phosphotransferase system, composed of DgaABCD. The protein product of dgaE, d-glucosaminate-6-phosphate ammonia lyase (DGL), converts G6P to 2-keto-3-deoxygluconate-6-phosphate, which undergoes a retroaldol reaction catalyzed by the DgaF protein to give d-glyceraldehyde-3-phosphate and pyruvate...
December 22, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29276172/dysbiosis-associated-change-in-host-metabolism-generates-lactate-to-support-salmonella-growth
#6
Caroline C Gillis, Elizabeth R Hughes, Luisella Spiga, Maria G Winter, Wenhan Zhu, Tatiane Furtado de Carvalho, Rachael B Chanin, Cassie L Behrendt, Lora V Hooper, Renato L Santos, Sebastian E Winter
During Salmonella-induced gastroenteritis, mucosal inflammation creates a niche that favors the expansion of the pathogen population over the microbiota. Here, we show that Salmonella Typhimurium infection was accompanied by dysbiosis, decreased butyrate levels, and substantially elevated lactate levels in the gut lumen. Administration of a lactate dehydrogenase inhibitor blunted lactate production in germ-free mice, suggesting that lactate was predominantly of host origin. Depletion of butyrate-producing Clostridia, either through oral antibiotic treatment or as part of the pathogen-induced dysbiosis, triggered a switch in host cells from oxidative metabolism to lactate fermentation, increasing both lactate levels and Salmonella lactate utilization...
December 15, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/29249317/chicken-macrophages-infected-with-salmonella-s-enteritidis-or-s-heidelberg-produce-differential-responses-in-immune-and-metabolic-signaling-pathways
#7
Haiqi He, Ryan J Arsenault, Kenneth J Genovese, Casey Johnson, Michael H Kogut
Protein kinases act in coordination with phosphatases to control protein phosphorylation and regulate signaling pathways and cellular processes involved in nearly every functions of cell life. Salmonella are known to manipulate the host kinase network to gain entrance and survive inside host cells. The effect of Salmonella infection on the host kinase network has been studied in mammalian cells, but information is largely lacking in chicken immune cells. Our previous study indicated that chicken macrophage cells respond differentially to different Salmonella strains...
January 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29247761/genotoxicity-assessment-of-biphasic-calcium-phosphate-of-modified-porosity-on-human-dental-pulp-cells-using-ames-and-comet-assays
#8
Nur Fathin Alia Che Wahab, Thirumulu Ponnuraj Kannan, Zuliani Mahmood, Ismail Ab Rahman, Hanafi Ismail
Biphasic Calcium Phosphate (BCP) with a ratio of 20/80 Hydroxyapatite (HA)/Beta-tricalcium phosphate (β-TCP) promotes the differentiation of human dental pulp cells (HDPCs). In the current study, the genotoxicity of locally produced BCP of modified porosity (65%) with a mean pore size of 300micrometer (μm) was assessed using Comet and Ames assays. HDPCs were treated with BCP extract at three different inhibitory concentrations which were obtained based on cytotoxicity test conducted with concurrent negative and positive controls...
December 13, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29247060/interactions-of-salmonella-enterica-sv-typhimurium-and-pectobacterium-carotovorum-within-a-tomato-soft-rot
#9
Andrée S George, Clayton E Cox, Prerak Desai, Steffen Porwolik, Weiping Chu, Marcos H de Moraes, Michael McClelland, Maria T Brandl, Max Teplitski
The human pathogen Salmonella is remarkably adaptable, and this trait allows these bacteria to thrive in a variety of environments and hosts. The mechanisms with which these pathogens establish within a niche amidst the native microbiota remains poorly understood. Here, we aimed to uncover the mechanisms that enable Salmonella enterica sv. Typhimurium to benefit from the degradation of plant tissue by a soft-rot plant pathogen Pectobacterium carotovorum. The hypothesis that in the soft rot, the liberation of starch (not utilized by P...
December 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29244123/emergence-and-genomic-analysis-of-mdr-laribacter-hongkongensis-strain-hlgz1-from-guangzhou-china
#10
Hong-Kai Wu, Jian-Huan Chen, Ling Yang, A-Rong Li, Dan-Hong Su, Yong-Ping Lin, Ding-Qiang Chen
Background: Laribacter hongkongensis is a facultative anaerobic, non-fermentative, Gram-negative bacillus associated with community-acquired gastroenteritis and traveller's diarrhoea. No clinical MDR L. hongkongensis isolate has been reported yet. Methods: We performed WGS (PacBio and Illumina) on a clinical L. hongkongensis strain HLGZ1 with an MDR phenotype. Results: HLGZ1 was resistant to eight classes of commonly used antibiotics. Its complete genome was a single circular chromosome of 3 424 272 bp with a G + C content of 62...
December 13, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29229736/comparison-of-salmonella-enterica-serovars-typhi-and-typhimurium-reveals-typhoidal-specific-responses-to-bile
#11
Rebecca Johnson, Matt Ravenhall, Derek Pickard, Gordon Dougan, Alexander Byrne, Gad Frankel
Salmonella enterica serovars Typhi and Typhimurium cause typhoid fever and gastroenteritis respectively. A unique feature of typhoid infection is asymptomatic carriage within the gallbladder, which is linked with S Typhi transmission. Despite this, S Typhi responses to bile have been poorly studied. RNA-Seq of S Typhi Ty2 and a clinical S Typhi isolate belonging to the globally dominant H58 lineage (129-0238), as well as S Typhimurium 14028, revealed that 249, 389 and 453 genes respectively were differentially expressed in the presence of 3% bile compared to control cultures lacking bile...
December 11, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/29222755/integrated-host-pathogen-metabolic-reconstructions
#12
Anu Raghunathan, Neema Jamshidi
The science and art of Genome scale metabolic network reconstructions have been explicitly documented in the literature for organisms across all the three kingdoms of life. Constraints-based models derived from such reconstructions have been used to assess metabolic phenotypes of their complex connections to genotype accurately. The problem of infectious disease is complex due to the multifactorial response of the host to the pathogen. Systems biology approaches and modeling allow one to study, understand, and predict emergent properties of such complex responses...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29187844/speg-is-required-for-intracellular-replication-of-salmonella-in-various-human-cells-and-affects-its-polyamine-metabolism-and-global-transcriptomes
#13
Shiuh-Bin Fang, Ching-Jou Huang, Chih-Hung Huang, Ke-Chuan Wang, Nai-Wen Chang, Hung-Yin Pan, Hsu-Wei Fang, Ming-Te Huang, Ching-Kuo Chen
The speG gene has been reported to regulate polyamine metabolism in Escherichia coli and Shigella, but its role in Salmonella remains unknown. Our preliminary studies have revealed that speG widely affects the transcriptomes of infected in vitro M and Caco-2 cells and that it is required for the intracellular replication of Salmonella enterica serovar Typhimurium (S. Typhimurium) in HeLa cells. In this study, we demonstrated that speG plays a time-dependent and cell type-independent role in the intracellular replication of S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29183286/first-gene-ontology-enrichment-analysis-based-on-bacterial-coregenome-variants-insights-into-adaptations-of-salmonella-serovars-to-mammalian-and-avian-hosts
#14
Arnaud Felten, Meryl Vila Nova, Kevin Durimel, Laurent Guillier, Michel-Yves Mistou, Nicolas Radomski
BACKGROUND: Many of the bacterial genomic studies exploring evolution processes of the host adaptation focus on the accessory genome describing how the gains and losses of genes can explain the colonization of new habitats. Consequently, we developed a new approach focusing on the coregenome in order to describe the host adaptation of Salmonella serovars. METHODS: In the present work, we propose bioinformatic tools allowing (i) robust phylogenetic inference based on SNPs and recombination events, (ii) identification of fixed SNPs and InDels distinguishing homoplastic and non-homoplastic coregenome variants, and (iii) gene-ontology enrichment analyses to describe metabolic processes involved in adaptation of Salmonella enterica subsp...
November 28, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/29163445/in-silico-analysis-of-putrefaction-pathways-in-bacteria-and-its-implication-in-colorectal-cancer
#15
Harrisham Kaur, Chandrani Das, Sharmila S Mande
Fermentation of undigested proteins in human gastrointestinal tract (gut) by the resident microbiota, a process called bacterial putrefaction, can sometimes disrupt the gut homeostasis. In this process, essential amino acids (e.g., histidine, tryptophan, etc.) that are required by the host may be utilized by the gut microbes. In addition, some of the products of putrefaction, like ammonia, putrescine, cresol, indole, phenol, etc., have been implicated in the disease pathogenesis of colorectal cancer (CRC). We have investigated bacterial putrefaction pathways that are known to be associated with such metabolites...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163440/nutrient-composition-promotes-switching-between-pellicle-and-bottom-biofilm-in-salmonella
#16
Sonia Paytubi, Cintia Cansado, Cristina Madrid, Carlos Balsalobre
Salmonella is one of the most frequently reported causes of foodborne illness worldwide. Non-typhoidal serovars cause gastroenteritis in humans. Salmonella can grow on surfaces forming biofilms, contributing to its persistence since biofilms are difficult to eradicate due to the high resistance to antimicrobials and disinfectants. It has been described that there are two crucial biofilm promoting factors in Salmonella: curli and cellulose. The expression of both factors is coordinately regulated by the transcriptional regulator CsgD...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29155076/genotoxicity-and-28-day-oral-toxicity-studies-of-a-functional-food-mixture-containing-maltodextrin-white-kidney-bean-extract-mulberry-leaf-extract-and-niacin-bound-chromium-complex
#17
Cheng-Tien Wu, Chen-Yuan Chiu, Chun-Fa Huang, Fu-Chuo Peng, Shing-Hwa Liu
Steady-fiber granule (SFG) is a functional food mixture that is composed of four major ingredients, resistant maltodextrin, white kidney bean (Phaseolus vulgaris) extract, mulberry leaf (Morus alba L.) extract, and niacin-bound chromium complex. This study focused on determining the safety of SFG. Genotoxicity and 28-day oral toxicity were evaluated. SFG did not induce mutagenicity in the bacterial reverse mutation assay using five Salmonella typhimurium strains (TA98, TA100, TA102, TA1535, and TA1537) in the presence or absence of metabolic activation (S9 system)...
November 16, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/29148923/ames-and-random-amplified-polymorphic-dna-tests-for-the-validation-of-the-mutagenic-and-or-genotoxic-potential-of-the-drinking-water-disinfection-by-products-chloroform-and-bromoform
#18
Messaouda Khallef, Süleyman Cenkci, Dilek Akyil, Arzu Özkara, Muhsin Konuk, Djamel Eddine Benouareth
Chloroform and Bromoform are two abundant trihalomethanes found in Algerian drinking water. The investigation of the mutagenic hazard of these disinfection by-products was studied by Ames test as prokaryotic bioassay to show their mutagenic effects. For this, Salmonella typhimurium TA98 and TA100 strains were employed. Both chloroform and bromoform showed a direct mutagenic effect since the number of revertant colonies gradually increase in dose-dependent manner with all concentrations tested with the two bacterial strains and these were both in the absence and presence of S9 metabolic activation...
November 17, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/29127378/first-insights-into-the-pleiotropic-role-of-vrf-yedf-a-newly-characterized-gene-of-salmonella-typhimurium
#19
Clara Ballesté-Delpierre, Dietmar Fernandez-Orth, Mario Ferrer-Navarro, Ramón Díaz-Peña, Antonia Odena-Caballol, Eliandre Oliveira, Anna Fàbrega, Jordi Vila
Salmonella possesses virulence determinants that allow replication under extreme conditions and invasion of host cells, causing disease. Here, we examined four putative genes predicted to encode membrane proteins (ydiY, ybdJ, STM1441 and ynaJ) and a putative transcriptional factor (yedF). These genes were identified in a previous study of a S. Typhimurium clinical isolate and its multidrug-resistant counterpart. For STM1441 and yedF a reduced ability to interact with HeLa cells was observed in the knock-out mutants, but an increase in this ability was absent when these genes were overexpressed, except for yedF which phenotype was rescued when yedF was restored...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29117268/general-response-of-salmonella-enterica-serovar-typhimurium-to-desiccation-a-new-role-for-the-virulence-factors-sopd-and-ssed-in-survival
#20
Alice Maserati, Ryan C Fink, Antonio Lourenco, Matthew L Julius, Francisco Diez-Gonzalez
Salmonella can survive for long periods under extreme desiccation conditions. This stress tolerance poses a risk for food safety, but relatively little is known about the molecular and cellular regulation of this adaptation mechanism. To determine the genetic components involved in Salmonella's cellular response to desiccation, we performed a global transcriptomic analysis comparing S. enterica serovar Typhimurium cells equilibrated to low water activity (aw 0.11) and cells equilibrated to high water activity (aw 1...
2017: PloS One
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