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https://www.readbyqxmd.com/read/28924030/altered-regulation-of-the-diguanylate-cyclase-yaic-reduces-production-of-type-1-fimbriae-in-a-pst-mutant-of-uropathogenic-e-coli-cft073
#1
Sébastien Crépin, Gaëlle Porcheron, Sébastien Houle, Josée Harel, Charles M Dozois
The pst gene cluster encodes the phosphate specific transport system (Pst). Inactivation of the Pst system constitutively activates the two-component regulatory system PhoBR and attenuates virulence of pathogenic bacteria. In uropathogenic E. coli strain CFT073, attenuation by inactivation of pst is predominantly attributed to the decreased expression of type 1 fimbriae. However, the molecular mechanisms connecting the Pst system and type 1 fimbriae are unknown. To address this, a transposon library was constructed in the pst mutant, and clones were tested for a regain in type 1 fimbriae production...
September 18, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28904956/application-of-the-subtractive-genomics-and-molecular-docking-analysis-for-the-identification-of-novel-putative-drug-targets-against-salmonella-enterica-subsp-enterica-serovar-poona
#2
Tanvir Hossain, Mohammad Kamruzzaman, Talita Zahin Choudhury, Hamida Nooreen Mahmood, A H M Nurun Nabi, Md Ismail Hosen
The emergence of novel pathogenic strains with increased antibacterial resistance patterns poses a significant threat to the management of infectious diseases. In this study, we aimed at utilizing the subtractive genomic approach to identify novel drug targets against Salmonella enterica subsp. enterica serovar Poona strain ATCC BAA-1673. We employed in silico bioinformatics tools to subtract the strain-specific paralogous and host-specific homologous sequences from the bacterial proteome. The sorted proteome was further refined to identify the essential genes in the pathogenic bacterium using the database of essential genes (DEG)...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28892017/biodiversity-of-actinobacteria-from-the-south-pacific-and-the-assessment-of-streptomyces-chemical-diversity-with-metabolic-profiling
#3
Andrés Cumsille, Agustina Undabarrena, Valentina González, Fernanda Claverías, Claudia Rojas, Beatriz Cámara
Recently, bioprospecting in underexplored habitats has gained enhanced focus, since new taxa of marine actinobacteria can be found, and thus possible new metabolites. Actinobacteria are in the foreground due to their versatile production of secondary metabolites that present various biological activities, such as antibacterials, antitumorals and antifungals. Chilean marine ecosystems remain largely unexplored and may represent an important source for the discovery of bioactive compounds. Various culture conditions to enrich the growth of this phylum were used and 232 bacterial strains were isolated...
September 11, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28883986/redox-active-conducting-polymers-modulate-salmonella-biofilm-formation-by-controlling-availability-of-electron-acceptors
#4
Salvador Gomez-Carretero, Ben Libberton, Mikael Rhen, Agneta Richter-Dahlfors
Biofouling is a major problem caused by bacteria colonizing abiotic surfaces, such as medical devices. Biofilms are formed as the bacterial metabolism adapts to an attached growth state. We studied whether bacterial metabolism, hence biofilm formation, can be modulated in electrochemically active surfaces using the conducting conjugated polymer poly(3,4-ethylenedioxythiophene) (PEDOT). We fabricated composites of PEDOT doped with either heparin, dodecyl benzene sulfonate or chloride, and identified the fabrication parameters so that the electrochemical redox state is the main distinct factor influencing biofilm growth...
2017: NPJ Biofilms and Microbiomes
https://www.readbyqxmd.com/read/28879636/-cr3o-o2cch2ch3-6-h2o-3-no3%C3%A2-h2o-cr3-toxicity-potential-in-bacterial-and-mammalian-cells
#5
Lu Jiang, John B Vincent, Melissa M Bailey
Chromium(III) has generally been considered to be essential for proper carbohydrate and lipid metabolism, and, despite recent evidence to the contrary, chromium(III)-containing compounds remain one of the most popular commercial dietary supplements. Cr3, or [Cr3O(O2CCH2CH3)6(H2O)3]NO3·H2O, is a trivalent chromium compound that is a promising chromium nutritional supplement. Studies with Cr3 have indicated that it is non-toxic in developmental and short- and long-term exposure studies in rodents, but the safety of this compound to chromosomes and cells has not been explored...
September 7, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28859308/integration-host-factor-is-important-for-biofilm-formation-by-salmonella-enterica-enteritidis
#6
Bruna Leite, Catierine Hirsch Werle, Camila Pinheiro do Carmo, Diego Borin Nóbrega, Guilherme Paier Milanez, Hebert Fabricio Culler, Marcelo Palma Sircili, Cristina E Alvarez-Martinez, Marcelo Brocchi
Salmonella enterica Enteritidis forms biofilms and survives in agricultural environments, infecting poultry and eggs. Bacteria in biofilms are difficult to eradicate compared to planktonic cells, causing serious problems in industry and public health. In this study, we evaluated the role of ihfA and ihfB in biofilm formation by S. enterica Enteritidis by employing different microbiology techniques. Our data indicate that ihf mutant strains are impaired in biofilm formation, showing a reduction in matrix formation and a decrease in viability and metabolic activity...
August 31, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28854667/effect-of-dietary-nucleosides-and-yeast-extracts-on-composition-and-metabolic-activity-of-infant-gut-microbiota-in-polyferms-colonic-fermentation-models
#7
Eun-Hee Doo, Christophe Chassard, Clarissa Schwab, Christophe Lacroix
Nucleotides (NT) and nucleosides (NS) are added to infant formula to mimic the content of breast milk, but little is known about their impact on infant gut microbiota. In this study, we tested the effect of NS and of yeast extracts (YE) with different NT content using PolyFermS continuous fermentation models mimicking formula-fed, healthy and enteropathogen-contaminated infant gut microbiota. Microbiota composition, short-chain fatty acid (SCFA) formation and gene expression were determined. NS, and to a larger extend YE modulated microbiota composition and increased metabolic activity in both models...
August 1, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28844888/an-oxidative-central-metabolism-enables-salmonella-to-utilize-microbiota-derived-succinate
#8
Luisella Spiga, Maria G Winter, Tatiane Furtado de Carvalho, Wenhan Zhu, Elizabeth R Hughes, Caroline C Gillis, Cassie L Behrendt, Jiwoong Kim, Daniela Chessa, Helene L Andrews-Polymenis, Daniel P Beiting, Renato L Santos, Lora V Hooper, Sebastian E Winter
The mucosal inflammatory response induced by Salmonella serovar Typhimurium creates a favorable niche for this gut pathogen. Conventional wisdom holds that S. Typhimurium undergoes an incomplete tricarboxylic acid (TCA) cycle in the anaerobic mammalian gut. One change during S. Typhimurium-induced inflammation is the production of oxidized compounds by infiltrating neutrophils. We show that inflammation-derived electron acceptors induce a complete, oxidative TCA cycle in S. Typhimurium, allowing the bacteria to compete with the microbiota for colonization...
September 13, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28806780/the-arac-negative-regulator-family-modulates-the-activity-of-histone-like-proteins-in-pathogenic-bacteria
#9
Araceli E Santiago, Michael B Yan, Tracy H Hazen, Brooke Sauder, Mario Meza-Segura, David A Rasko, Melissa M Kendall, Fernando Ruiz-Perez, James P Nataro
The AraC Negative Regulators (ANR) comprise a large family of virulence regulators distributed among diverse clinically important Gram-negative pathogens, including Vibrio spp., Salmonella spp., Shigella spp., Yersinia spp., Citrobacter spp., and pathogenic E. coli strains. We have previously reported broad effects of the ANR members on regulators of the AraC/XylS family. Here, we interrogate possible broader effects of the ANR members on the bacterial transcriptome. Our studies focused on Aar (AggR-activated regulator), an ANR family archetype in enteroaggregative E...
August 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28799667/coadministration-of-isomalto-oligosaccharides-augments-metabolic-health-benefits-of-cinnamaldehyde-in-high-fat-diet-fed-mice
#10
Dhirendra Pratap Singh, Pragyanshu Khare, Vandana Bijalwan, Ritesh Kumar Baboota, Jagdeep Singh, Kanthi Kiran Kondepudi, Kanwaljit Chopra, Mahendra Bishnoi
Bacteriostatic properties of a potential anti-obesity agent cinnamaldehyde (CMN) may present untoward effects on the resident gut microbiota. Here, we evaluated whether the combination of Isomalto-oligosaccharides (IMOs) with CMN prevents unwanted effects of CMN on gut microbiota and associated metabolic outcomes in HFD-fed mice. Male Swiss albino mice divided into four groups (n = 10), were fed on normal chow, or HFD (58% fat kcal), HFD + CMN (10 mg kg(-1) ) and HFD + CMN (10 mg kg(-1) ) + IMOs (1 g kg(-1) ) for 12 weeks...
August 11, 2017: BioFactors
https://www.readbyqxmd.com/read/28798330/metabolic-networks-for-nitrogen-utilization-in-prevotella-ruminicola-23
#11
Jong Nam Kim, Celia Méndez-García, Renae R Geier, Michael Iakiviak, Jongsoo Chang, Isaac Cann, Roderick I Mackie
Nitrogen metabolism in gut systems remains poorly studied in spite of its importance for microbial growth and its implications for the metabolism of the host. Prevotella spp. are the most predominant bacteria detected in the rumen, but their presence has also been related to health and disease states in the human gut and oral cavity. To explore the metabolic networks for nitrogen assimilation in this bacterium, changes in gene expression profiles in response to variations in the available nitrogen source and to different concentrations of ammonium were analyzed by microarray and reverse transcription quantitative PCR, and linked with function by further proteomic analysis...
August 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28798125/microbiota-activated-ppar-%C3%AE-signaling-inhibits-dysbiotic-enterobacteriaceae-expansion
#12
Mariana X Byndloss, Erin E Olsan, Fabian Rivera-Chávez, Connor R Tiffany, Stephanie A Cevallos, Kristen L Lokken, Teresa P Torres, Austin J Byndloss, Franziska Faber, Yandong Gao, Yael Litvak, Christopher A Lopez, Gege Xu, Eleonora Napoli, Cecilia Giulivi, Renée M Tsolis, Alexander Revzin, Carlito B Lebrilla, Andreas J Bäumler
Perturbation of the gut-associated microbial community may underlie many human illnesses, but the mechanisms that maintain homeostasis are poorly understood. We found that the depletion of butyrate-producing microbes by antibiotic treatment reduced epithelial signaling through the intracellular butyrate sensor peroxisome proliferator-activated receptor γ (PPAR-γ). Nitrate levels increased in the colonic lumen because epithelial expression of Nos2, the gene encoding inducible nitric oxide synthase, was elevated in the absence of PPAR-γ signaling...
August 11, 2017: Science
https://www.readbyqxmd.com/read/28796780/transcriptome-and-proteome-analysis-of-salmonella-enterica-serovar-typhimurium-systemic-infection-of-wild-type-and-immune-deficient-mice
#13
Olusegun Oshota, Max Conway, Maria Fookes, Fernanda Schreiber, Roy R Chaudhuri, Lu Yu, Fiona J E Morgan, Simon Clare, Jyoti Choudhary, Nicholas R Thomson, Pietro Lio, Duncan J Maskell, Pietro Mastroeni, Andrew J Grant
Salmonella enterica are a threat to public health. Current vaccines are not fully effective. The ability to grow in infected tissues within phagocytes is required for S. enterica virulence in systemic disease. As the infection progresses the bacteria are exposed to a complex host immune response. Consequently, in order to continue growing in the tissues, S. enterica requires the coordinated regulation of fitness genes. Bacterial gene regulation has so far been investigated largely using exposure to artificial environmental conditions or to in vitro cultured cells, and little information is available on how S...
2017: PloS One
https://www.readbyqxmd.com/read/28790395/knockdown-of-hif-1%C3%AE-by-sirna-expressing-plasmid-delivered-by-attenuated-salmonella-enhances-the-antitumor-effects-of-cisplatin-on-prostate-cancer
#14
Junlian Gu, Yang Li, Jun Zeng, Bo Wang, Kun Ji, Yufeng Tang, Qing Sun
Resistance to cisplatin (DDP) and dose-related toxicity remain two important obstacles in the treatment of prostate cancer (PCa) patients with DDP-based chemotherapy. We have investigated whether the knockdown of hypoxia-inducible factor-1 alpha (HIF-1α) by siRNA could enhance the antitumor activity of DDP, and aimed to determine the underlying mechanisms. Intravenous injection of attenuated Salmonella carrying a HIF-1α siRNA-expressing plasmid was used to knockdown HIF-1α in a PC-3 xenograft model. The in vitro and in vivo effects of HIF-1α siRNA treatment and/or DPP on PCa cell proliferation, apoptosis, glycolysis, and production of reactive oxygen species (ROS) were assessed by examining molecular markers specific to each process...
August 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28699507/quinolones-and-fluroquinolones-metabolism-and-resistance-in-salmonella-typhimurium-a-mini-review
#15
B Preethi, V Shanthi, K Ramanathan
Salmonella enterica serovars causes substantial morbidity and mortality worldwide. More than 2500 different serotypes of Salmonella were identified to date. In particular, Salmonella enterica serovar typhimurium is the most important serotype of Salmonella transmitted from animals to humans in most parts of the world. They are associated with an estimated 1 million deaths annually. Quinolones are an important class of broad-spectrum antimicrobials has been utilized in the treatment option for over 40 years...
July 10, 2017: Current Drug Metabolism
https://www.readbyqxmd.com/read/28698862/antimutagenic-and-antioxidant-activities-of-thai-rice-brans
#16
Orapin Insuan, Arpamas Chariyakornkul, Yuwada Rungrote, Rawiwan Wongpoomchai
BACKGROUND: Rice bran is the outer layer of the rice grain, and contains high amounts of bioactive phytochemicals. Here, we investigated and compared chemopreventive properties of purple and white rice bran extracts. METHODS: Rice bran was extracted with dichloromethane and methanol. Chemical constituents in the extracts were analyzed by colorimetric assay and high performance liquid chromatography. The mutagenicity and antimutagenicity of the extracts were determined via the Salmonella mutation assay...
June 2017: Journal of Cancer Prevention
https://www.readbyqxmd.com/read/28682593/selective-and-high-dynamic-range-assay-format-for-multiplex-detection-of-pathogenic-pseudomonas-aeruginosa-salmonella-typhimurium-and-legionella-pneumophila-rnas-using-surface-plasmon-resonance-imaging
#17
F Melaine, M Saad, S Faucher, M Tabrizian
Due to its well-characterized and highly conserved structure, as well as its relative abundance in metabolically active cells, bacterial 16S rRNA sequence plays an important role in microbial identification. In this work, a biosensing strategy has been developed for simultaneous detection of 16S rRNA analytes of three pathogenic bacterial strains: Legionella pneumophila, Pseudomonas aeruginosa, and Salmonella typhimurium. Surface plasmon resonance imaging (SPRi) was used as a detection technique coupled with DNA probe sandwich assemblies and gold nanoparticles (GNPs) for signal amplification...
July 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28674150/temporal-regulation-of-a-salmonella-typhimurium-virulence-factor-by-the-transcriptional-regulator-ydcr
#18
Yanhua Liu, Qian Liu, Linlu Qi, Tao Ding, Zhen Wang, Jiaqi Fu, Mo Hu, Min Li, Jeongmin Song, Xiaoyun Liu
We previously examined Salmonella proteome within infected host cells and found differential expression of many proteins with defined functional roles such as metabolism or virulence. However, the precise roles of other altered proteins in Salmonella pathogenesis are largely unknown. A putative transcriptional regulator, YdcR, was highly induced intracellularly whereas barely expressed in vitro, implicating potential relevance to bacterial infection. To unveil its physiological functions, we exploited quantitative proteomics of intracellular Salmonella and found that genetic ablation of ydcR resulted in severe repression of SrfN, a known virulence factor...
September 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28674031/role-of-sapa-and-yfga-in-susceptibility-to-antibody-mediated-complement-dependent-killing-and-virulence-of-salmonella-enterica-serovar-typhimurium
#19
Edna M Ondari, Jennifer N Heath, Elizabeth J Klemm, Gemma Langridge, Lars Barquist, David A Goulding, Simon Clare, Gordon Dougan, Robert A Kingsley, Calman A MacLennan
The ST313 pathovar of Salmonella enterica serovar Typhimurium contributes to a high burden of invasive disease among African infants and HIV-infected adults. It is characterized by genome degradation (loss of coding capacity) and has increased resistance to antibody-dependent complement-mediated killing compared with enterocolitis-causing strains of S Typhimurium. Vaccination is an attractive disease-prevention strategy, and leading candidates focus on the induction of bactericidal antibodies. Antibody-resistant strains arising through further gene deletion could compromise such a strategy...
September 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28672057/intracellular-salmonella-metabolism
#20
REVIEW
Dirk Bumann, Joep Schothorst
Growth of Salmonella inside infected host cells is a key aspect of their ability to cause local enteritis or systemic disease. This growth depends on exploitation of host nutrients through a large Salmonella metabolism network with hundreds of metabolites and enzymes. Studies in cell culture infection models are unravelling more and more of the underlying molecular and cellular mechanisms but also show striking Salmonella metabolic plasticity depending on host cell line and experimental conditions. In vivo studies have revealed a qualitatively diverse, but quantitatively poor, host-Salmonella nutritional interface, which on one side makes Salmonella fitness largely resilient against metabolic perturbations, but on the other side severely limits Salmonella biomass generation and growth rates...
July 3, 2017: Cellular Microbiology
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