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giardia lamblia

Eleuza Rodrigues Machado, Núbia Oliveira Matos, Sinione Morais Rezende, Daniela Carlos, Thauana Cristina Silva, Leônia Rodrigues, Maria Jarlene Rodrigues Almeida, Maria Regina Fernandes de Oliveira, Maria Imaculada Muniz-Junqueira, Rodrigo Gurgel-Gonçalves
Host-parasite interactions in diabetic patients might influence diabetes complications and intestinal parasitosis. The aim was to investigate the occurrence of enteroparasites in individuals with diabetes types 1 and 2. A descriptive study was designed to estimate frequencies of parasites and to compare them in individuals with diabetes types 1 and 2 from two Health Centers and one hospital in the Federal District of Brazil. Patients were allocated to the study by convenience. Three fecal samples of 156 diabetic individuals (120 type 1 and 36 type 2) were analyzed using two parasitological methods...
2018: Journal of Diabetes Research
Dibyendu Raj, Punam Chowdhury, Rituparna Sarkar, Yumiko Saito-Nakano, Keinosuke Okamoto, Shanta Dutta, Tomoyoshi Nozaki, Sandipan Ganguly
Giardia lamblia , an anaerobic, amitochondriate protozoan parasite causes parasitic infection giardiasis in children and young adults. It produces pyruvate, a major metabolic product for its fermentative metabolism. The current study was undertaken to explore the effects of pyruvate as a physiological antioxidant during oxidative stress in Giardia by cysteine-ascorbate deprivation and further investigation upon the hypothesis that oxidative stress due to metabolism was the reason behind the cytotoxicity. We have estimated intracellular reactive oxygen species generation due to cysteine-ascorbate deprivation in Giardia ...
February 2018: Korean Journal of Parasitology
N I Bi-Xian, S Ming-Xue, X U Xiang-Zhen, W Xiao-Ting, D Yang, J Xiao-Lin
Objective To know the contamination status of Giardia lamblia and Cryptosporidium in drinking water of Jiangsu Province, so as to provide the evidence for producing hygiene and safety drinking water. Methods A total of 28 water plants of 13 cities in Jiangsu Province were selected, and the source water (10 L), chlorinated water (100 L) and tap water (100 L) were collected separately in each site. The water samples were then treated by filtration, washing, centrifuging concentration, immune magnetic separation, and immunofluorescent assay, to detect the numbers of Giardia cysts and Cryptosporidium oocysts...
May 17, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
Zhang Le-Sheng, Wang Yan-Juan, Cao Jian-Ping
Giardia lamblia is an important intestinal protozoan which can cause diarrhea in humans. The detection of Giardia infection is performed through the detection methods of pathogen, immunoassay and molecular biology. Currently, the immunodiagnostic methods have good application and development prospect because of high sensitivity and specificity, simple and convenient, and time saving. In this article, we review the main progress and application of immunodiagnostic methods for Giardia infection.
June 12, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
Theo A Schuurs, Rob Koelewijn, Eric A T Brienen, Titia Kortbeek, Theo G Mank, Bert Mulder, Foekje F Stelma, Lisette van Lieshout, Jaap J van Hellemond
BACKGROUND: Real-time PCR methods are increasingly used in routine patient care settings not only to determine the presence or absence of pathogens in patient materials, but also to obtain semiquantitative results to estimate the pathogen load. However, it is so far unknown how well these methods are harmonized among different laboratories. METHODS: Sets of stool samples were distributed three to four times per year to ca. 25-40 participating laboratories within the European Union as part of an external quality assessment scheme (EQAS) for the detection of gastrointestinal protozoa...
February 16, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
Carolina Gasparinho, Filipa S Ferreira, António Carlos Mayer, Maria Clara Mirante, Susana Vaz Nery, Ana Santos-Reis, Daniela Portugal-Calisto, Miguel Brito
Background: Giardia lamblia is a pathogenic intestinal protozoan with high prevalence in developing countries, especially among children. Molecular characterization has revealed the existence of eight assemblages, with A and B being more commonly described in human infections. Despite its importance, to our knowledge this is the first published molecular analysis of G. lamblia assemblages in Angola. Methods: The present study aimed to identify the assemblages of G...
February 9, 2018: Transactions of the Royal Society of Tropical Medicine and Hygiene
Akua Obeng Forson, Isaac Arthur, Patrick F Ayeh-Kumi
INTRODUCTION: Intestinal parasitic infections (IPIs) in school children are a public health problem in most developing countries. METHODS AND PRINCIPAL FINDINGS: A cross sectional survey was conducted from May to July 2016 with school children living in overcrowded urban slums in Accra, Ghana. A simple random sample of 300 children aged 2-9 years was collected. The study used structured pre-tested questionnaire and stool tests to obtain information on epidemiological, sanitation habits, employment and education status of parents and children...
2018: PloS One
Shumin Xiao, Yan Zhang, Xiaoyun Zhao, Liping Sun, Sike Hu
Little is known about the occurrence of Cryptosporidium and Giardia in recreational water in China. A total of 52 samples were collected from recreational lakes in Tianjin during a high-occurrence season (June-October) for the waterborne cryptosporidiosis and giardiasis, and the occurrence and genotypes of Cryptosporidium and Giardia were investigated. The results showed that 82.7% (43) and 98.1% (51) of samples were positive for Cryptosporidium oocyst and Giardia cysts, respectively. The mean concentration of parasites was 3...
February 5, 2018: Scientific Reports
Ibrahim Hamad, Rita Abou Abdallah, Isabelle Ravaux, Saadia Mokhtari, Hervé Tissot-Dupont, Caroline Michelle, Andreas Stein, Jean-Christophe Lagier, Didier Raoult, Fadi Bittar
Research on the relationship between changes in the gut microbiota and human disease, including AIDS, is a growing field. However, studies on the eukaryotic component of the intestinal microbiota have just begun and have not yet been conducted in HIV-infected patients. Moreover, eukaryotic community profiling is influenced by the use of different methodologies at each step of culture-independent techniques. Herein, initially, four DNA extraction protocols were compared to test the efficiency of each method in recovering eukaryotic DNA from fecal samples...
2018: PloS One
Sverre Litleskare, Guri Rortveit, Geir Egil Eide, Kurt Hanevik, Nina Langeland, Knut-Arne Wensaas
BACKGROUND & AIMS: Irritable bowel syndrome (IBS) is a complication that can follow gastrointestinal infection, but it is not clear if patients also develop chronic fatigue. We investigated the prevalence and odds ratio of IBS and chronic fatigue 10 years after an outbreak of Giardia lamblia, compared with a control cohort, and changes in prevalence over time. METHODS: We performed a prospective follow-up study of 1252 laboratory-confirmed cases of giardiasis (exposed), which developed in Bergen, Norway in 2004...
January 25, 2018: Clinical Gastroenterology and Hepatology
Elena Chiappini, Mauro Zaffaroni, Martina Bianconi, Giuseppina Veneruso, Nicolino Grasso, Silvia Garazzino, Rosangela Arancio, Piero Valentini, Anna Ficcadenti, Maria Rosalia Da Riol, Simona La Placa, Luisa Galli, Maurizio de Martino, Gianni Bona
AIM: This study evaluated the prevalence of infectious diseases and immunisation status of children adopted from Africa METHODS: We studied 762 African children referred to 11 Italian paediatric centres in 2009-2015. Clinical and laboratory data were retrospectively collected and analysed. RESULTS: The median age of the children (60.3% males) was 3.6 years, 52.6% came from Ethiopia and 50.1% had at least one infectious disease. Parasitic infections accounted for the majority of the infectious diseases (409/715) and the most common were Giardia lamblia (n=239), Toxocara canis (n=65) and skin infections (n=205), notably Tinea capitis/corporis (n=134) and Molluscum contagiosum (n=56) Active tuberculosis (TB) was diagnosed in nine children (1...
January 24, 2018: Acta Paediatrica
Telanesh Zemene, Melashu Balew Shiferaw
OBJECTIVE: Intestinal parasitic infection is one of the major childhood health problems in developing countries. In Ethiopia, epidemiological data for several localities is limited. Hence, the aim of this study is to assess intestinal parasitic infections among under-five children attending in Debre Birhan referral hospital, which could help to decrease morbidity and mortality in children. A cross-sectional study was conducted in February, 2014. Stool specimens were collected and examined using concentration method...
January 22, 2018: BMC Research Notes
Gaetano Cristian Morreale, Luigi Maria Montalbano, Maria Cappello, Emanuele Sinagra, Aroldo Rizzo, Antonio Carroccio
Villous atrophy in absence of coeliac disease (CD)-specific antibodies represents a diagnostic dilemma. We report a case of a woman with anaemia, weight loss and diarrhoea with an initial diagnosis of seronegative CD and a histological documented villous atrophy who did not improve on gluten-free diet due to the concomitant presence of common variable immunodeficiency (CVID) and Giardia lamblia infection. This case report confirms that CD diagnosis in CVID patients is difficult; the combination of anti-endomysial antibodies (EmA-IgA), anti-tissue transglutaminase antibodies (tTG-IgAb) antibodies and total IgA is obligatory in basic diagnostic of CD but in CVID are negative...
December 2017: Arab Journal of Gastroenterology: the Official Publication of the Pan-Arab Association of Gastroenterology
Mehrgan Heydari-Hengami, Yaghoob Hamedi, Majid Najafi-Asl, Khojasteh Sharifi-Sarasiabi
Background: Food handlers play a significant role in the dissemination and transmission of enteropathogenic microorganisms, including intestinal parasites. The aim of this study was to determine the prevalence of intestinal parasites in food handlers of Bandar Abbas, southern Iran. Methods: In this cross-sectional study, 800 stool samples were randomly collected in a period of 6 months from Jun to Nov 2015. The diagnosis was made on direct wet mount, formalin-ether concentration, Ziehl-Neelsen, and Trichrome stained slides...
January 2018: Iranian Journal of Public Health
Mohammad Rayani, Gholamreza Hatam, Ngah Zasmy Unyah, Abdolmajid Ashrafmansori, Wan Omar Abdullah, Rukman Awang Hamat
Background: This study is the first phylogenetic genotype analysis of Giardia lamblia in Iran. The main objective was to determine genotyping and identify the sub-assemblages of Giardia lamblia isolates involved in the transmission of giardiasis in Fars Province, south of Iran, in 2012. Methods: Forty G. lamblia isolates were collected from the patient's fecal samples with gastrointestinal discomfort referred to the health centers and hospitals in Shiraz, Fars Province, south of Iran...
October 2017: Iranian Journal of Parasitology
Jaruwan Siritapetawee, Wanwisa Limphirat, Nawarat Nantapong, Dujdow Songthamwat
Recently, the development of 'green' methods for fabrication of silver nanoparticles (Ag-NPs) has been emphasized, in view of their environmental safety, feasibility and low cost. In this study, a serine protease, EuP-82 from Euphorbia cf. lactea latex, was used to fabricate silver chloride nanoparticles (AgCl-NPs) in phosphate-buffered saline (pH 7.2), under the influence of visible light. The fabricated nanoparticles had a maximal surface plasmon resonance absorption peak at 435 nm. The size of the AgCl-NPs, estimated by scanning electron microscopy, was 57 ± 14...
January 3, 2018: Biotechnology and Applied Biochemistry
Jintana Yanola, Woottichai Nachaiwieng, Suwit Duangmano, Mujalin Prasannarong, Pradya Somboon, Sakorn Pornprasert
Intestinal parasitic infection represents a substantial problem for children living in rural or limited resources areas and significantly relates to anemia and nutritional status. This study aimed to determine the current prevalence of intestinal parasitic infections among school-age children of Karen hill tribe population in Omkoi District, Chiang Mai Province, Thailand and assess the impact of intestinal parasitic infection on hematological and nutritional status in those children. A total of 375 Karen hill tribe children, 6-14 years of age, in Omkoi District were randomly selected to participate in this study...
January 5, 2018: Acta Tropica
Giselle DeVetten, Meghan Dirksen, Robert Weaver, Tanvir Turin Chowdhury, Michael William Aucoin
OBJECTIVE: To determine the prevalence of intestinal parasites and rates of stool testing compliance, as well as associated patient characteristics, among newly arrived refugees at the Mosaic Refugee Health Clinic in Calgary, Alta. DESIGN: Retrospective chart review. SETTING: Primary care clinic for refugee patients. PARTICIPANTS: A total of 1390 new refugee patients at the clinic from May 1, 2011, to June 30, 2013. MAIN OUTCOME MEASURES: Stool ova and parasite test completion and proportion of positive test results...
December 2017: Canadian Family Physician Médecin de Famille Canadien
Rainer Gosert, Ulrich Heininger, Hans H Hirsch
Acute gastroenteritis (GE) has a major impact on morbidity and mortality worldwide, yet comprehensive data regarding infectious agents including enteroviruses are scarce. We hypothesized that enteroviruses constitute a significant cause of acute GE. We analyzed 677 stool samples from 504 patients, which had been submitted for suspected infectious GE. 0.2 ml of stool suspension was extracted using the Abbott m2000 sp robot and analysed by multiplex nucleic acid testing (NAT) using the Luminex xTAG gastrointestinal pathogen panel (GPP) as well as by specific NATs detecting enteroviruses and polioviruses...
December 13, 2017: Journal of Medical Virology
Nabanita Saha, Somnath Dutta, Shankari P Datta, Srimonti Sarkar
The ESCRT pathway functions at different subcellular membranes to induce their negative curvature, and it has been largely characterized in model eukaryotes belonging to Opisthokonta. But searches of the genomes of many nonopisthokonts belonging to various supergroups indicate that some of them may harbour fewer ESCRT components. Of the genomes explored thus far, one of the most minimal set of ESCRT components was identified in the human pathogen Giardia lamblia, which belongs to Excavata. Here we report that an ESCRT-mediated pathway most likely operates at the peripheral vesicles, which are located at the cell periphery and the bare zone of this protist...
January 2018: European Journal of Cell Biology
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