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https://www.readbyqxmd.com/read/29454860/velocity-landscape-correlation-resolves-multiple-flowing-protein-populations-from-fluorescence-image-time-series
#1
Elvis Pandžić, Asmahan Abu-Arish, Renee W Whan, John W Hanrahan, Paul W Wiseman
Molecular, vesicular and organellar flows are of fundamental importance for the delivery of nutrients and essential components used in cellular functions such as motility and division. With recent advances in fluorescence/super-resolution microscopy modalities we can resolve the movements of these objects at higher spatio-temporal resolutions and with better sensitivity. Previously, spatio-temporal image correlation spectroscopy has been applied to map molecular flows by correlation analysis of fluorescence fluctuations in image series...
February 15, 2018: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/29449324/stim1-c-amps-up-melanogenesis
#2
Jonathan Soboloff, Bojana Gligorijevic, M Raza Zaidi
No abstract text is available yet for this article.
February 15, 2018: EMBO Journal
https://www.readbyqxmd.com/read/29438745/an-automated-epifluorescence-microscopy-imaging-assay-for-the-identification-of-phospho-akt-level-modulators-in-breast-cancer-cells
#3
Elke Kaemmerer, Dane Turner, Amelia A Peters, Sarah J Roberts-Thomson, Gregory R Monteith
AKT is an enzyme of the PI3K/pAKT pathway, regulating proliferation and cell survival. High basal levels of active, phosphorylated AKT (pAKT) are associated with tumor progression and therapeutic resistance in some breast cancer subtypes, including HER2 positive breast cancers. Various stimuli can increase pAKT levels and elevated basal pAKT levels are a feature of PTEN-deficient breast cancer cell lines. The aim of this study was to develop an assay able to identify modulators of pAKT levels using an automated epifluorescence microscope and high content analysis...
February 10, 2018: Journal of Pharmacological and Toxicological Methods
https://www.readbyqxmd.com/read/29414804/microrna-223-targeting-stim1-inhibits-the-biological-behavior-of-breast-cancer
#4
Yanfang Yang, Zhansheng Jiang, Ning Ma, Bin Wang, Jun Liu, Lina Zhang, Lin Gu
BACKGROUND/AIMS: To investigate the cellular effects and clinical significance of microRNA-223 (miR-223) in breast cancer by targeting stromal interaction molecule1 (STIM1). METHODS: Breast cancer cell lines (T47D, MCF-7, SKB-R3, MDA-MB-231 and MDA-MB-435) and a normal breast epithelial cell line (MCF-10A) were prepared for this study. MiR-223 mimics, anti-miR-223 and pcDNA 3.1-STIM1 were transiently transfected into cancer cells independently or together, and then RT-qPCR was performed to detect the expressions of miR-223 and STIM1 mRNA, dual-luciferase reporter assay was conducted to examine the effects of miR-223 on STIM1, Western blotting was used to measure the expressions of the STIM1 proteins, MTT and Trans-well assays were performed to detect cell proliferation and invasion...
January 31, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29414068/rapid-nmr-scale-purification-of-15n-13c-isotope-labeled-recombinant-human-stim1-coiled-coil-fragments
#5
Petr Rathner, Michael Stadlbauer, Christoph Romanin, Marc Fahrner, Isabella Derler, Norbert Müller
We report a new NMR-scale purification procedure for two recombinant wild type fragments of the stromal interaction molecule 1 (STIM1). This protein acts as a calcium sensor in the endoplasmic reticulum (ER) and extends into the cytosol accumulating at ER - plasma membrane (PM) junctions upon calcium store depletion ultimately leading to activation of the Orai/CRAC channel. The functionally relevant cytosolic part of STIM1 consists of three coiled coil domains, which are mainly involved in intra- and inter-molecular homomeric interactions as well as coupling to and gating of CRAC channels...
February 1, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29365044/calcium-oscillations-in-fertilized-pig-oocytes-are-associated-with-repetitive-interactions-between-stim1-and-orai1
#6
Lu Zhang, Chi-Hong Chao, Laurie A Jaeger, Agnes Bali Papp, Zoltan Machaty
The Ca2+ entry mechanism that sustains the Ca2+ oscillations in fertilized pig oocytes was investigated. STIM1 and ORAI1 proteins tagged with various fluorophores were expressed in the oocytes. In some cells, the Ca2+ stores were depleted using cyclopiazonic acid (CPA); others were inseminated. Changes in the oocytes' cytosolic free Ca2+ concentration were monitored, while interaction between the expressed fusion proteins was investigated using fluorescence resonance energy transfer (FRET). Store depletion led to an increase of the FRET signal in oocytes co-expressing mVenus-STIM1 and mTurquoise2-ORAI1, indicating that Ca2+ release was followed by an interaction between these proteins...
January 22, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29363328/molecular-physiology-and-pathophysiology-of-stromal-interaction-molecules
#7
Heather A Nelson, Michael W Roe
Ca2+ release from the endoplasmic reticulum is an important component of Ca2+ signal transduction that controls numerous physiological processes in eukaryotic cells. Release of Ca2+ from the endoplasmic reticulum is coupled to the activation of store-operated Ca2+ entry into cells. Store-operated Ca2+ entry provides Ca2+ for replenishing depleted endoplasmic reticulum Ca2+ stores and a Ca2+ signal that regulates Ca2+-dependent intracellular biochemical events. Central to connecting discharge of endoplasmic reticulum Ca2+ stores following G protein-coupled receptor activation with the induction of store-operated Ca2+ entry are stromal interaction molecules (STIM1 and STIM2)...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29360991/elevated-plasma-catecholamines-functionally-compensate-for-the-reduced-myogenic-tone-in-smooth-muscle-stim1-knockout-mice-but-with-deleterious-cardiac-effects
#8
Prahalathan Pichavaram, Wen Yin, Kirk W Evanson, Jonathan H Jaggar, Salvatore Mancarella
Aims: Stromal interaction molecule 1 (STIM1) has emerged as an important player in the regulation of growth and proliferation of smooth muscle cells. Therefore, we hypothesized that STIM1 plays a crucial role in the maintenance of vascular integrity. The objective of this study was to evaluate whether reduced expression of STIM1 could modify the structure and function of the vasculature, leading to changes in blood pressure (BP). Methods and results: Smooth muscle-specific STIM1 knockout (sm-STIM1 KO) in mice resulted in arteries with ∼80% reduced STIM1 protein expression as compared to control mice...
January 19, 2018: Cardiovascular Research
https://www.readbyqxmd.com/read/29359518/store-operated-calcium-entries-control-neural-stem-cell-self-renewal-in-the-adult-brain-subventricular-zone
#9
Florence Domenichini, Elodie Terrié, Patricia Arnault, Thomas Harnois, Christophe Magaud, Patrick Bois, Bruno Constantin, Valérie Coronas
The subventricular zone (SVZ) is the major stem cell niche in the brain of adult mammals. Within this region, neural stem cells proliferate, self-renew and give birth to neurons and glial cells. Previous studies underlined enrichment in calcium signaling-related transcripts in adult neural stem cells. Because of their ability to mobilize sustained calcium influxes in response to a wide range of extracellular factors, store-operated channels (SOC) appear to be, among calcium channels, relevant candidates to induce calcium signaling in neural stem cells whose cellular activities are continuously adapted to physiological signals from the microenvironment...
January 23, 2018: Stem Cells
https://www.readbyqxmd.com/read/29354237/novel-genetic-variants-associated-with-lumbar-spondylosis-in-koreans-a-genome-wide-association-study
#10
Hyun Ah Kim, Seong Gu Heo, Ji Wan Park, Young Ok Jung
Objective: The aim of this study was to identify the susceptibility genes responsible for lumbar spondylosis (LS) in Korean patients. Methods: Data from 1427 subjects were made available for radiographic grading and genome wide association studies (GWAS) analysis. Lateral lumbar spine radiographs were obtained and the various degrees of degenerative change were semi-quantitatively scored. A pilot GWAS was performed using the AffymetrixGenome-Wide Human single-nucleotide polymorphisms (SNPs), 500K array...
January 2018: Journal of Korean Neurosurgical Society
https://www.readbyqxmd.com/read/29352220/soce-induced-calcium-overload-regulates-autophagy-in-acute-pancreatitis-via-calcineurin-activation
#11
Zhen-Dong Zhu, Tao Yu, Hua-Jing Liu, Jing Jin, Jun He
Acute pancreatitis (AP) is an acute inflammatory process of the pancreas that is characterized by inflammation, edema, vacuolization and necrosis, which has significant morbidity and lethality. The pathogenesis of AP has not been established completely. An early and critical feature of AP is the aberrant signaling of Calcium (Ca2+) within the pancreatic acinar cell, termed Ca2+ overload. Store-operated Ca2+ (SOC) channels are the principal Ca2+ influx channels that contribute to Ca2+ overload in pancreatic acinar cells...
January 19, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29351410/hypoxia-selectively-upregulates-cation-channels-and-increases-cytosolic-ca2-in-pulmonary-but-not-coronary-arterial-smooth-muscle-cells
#12
Xi He, Shanshan Song, Ramon J Ayon, Angela Balisterieri, Stephen M Black, Ayako Makino, W Gil Wier, Wei-Jin Zang, Jason X-J Yuan
Ca2+ signaling, particularly the mechanism via store-operated Ca2+ entry (SOCE) and receptor-operated Ca2+ entry (ROCE), plays a critical role in the development of acute hypoxia-induced pulmonary vasoconstriction and chronic hypoxia-induced pulmonary hypertension. This study aimed to test the hypothesis that chronic hypoxia differentially regulates the expression of proteins that mediate SOCE and ROCE (STIM, Orai, and TRPC6) in pulmonary (PASMC) and coronary (CASMC) artery smooth muscle cells. The resting cytosolic [Ca2+] ([Ca2+]cyt) and the stored [Ca2+] in the sarcoplasmic reticulum (SR) were not different in CASMC and PASMC...
January 3, 2018: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29337674/a-study-on-blocking-store-operated-ca2-entry-in-pulmonary-arterial-smooth-muscle-cells-with-xyloketals-from-marine-fungi
#13
Jie-Bin Zhou, Ying-Ying Sun, Ying-Lin Zheng, Chu-Qin Yu, Hua-Qing Lin, Ji-Yan Pang
In this study, the effect of four xyloketals 1-4 on store-operated calcium entry (SOCE) was investigated in primary distal pulmonary arterial smooth muscle cells (PASMCs) isolated from mice. The results showed that xyloketal A (1), an unusual ketal with C-3 symmetry, exhibited strong SOCE blocking activity. Secretion of interleukin-8 (IL-8) was also inhibited by xyloketal A. The parallel artificial membrane permeability assay (PAMPA) of 1-4 suggested that these xyloketals penetrated easily through the cell membrane...
December 20, 2017: Acta Pharmaceutica
https://www.readbyqxmd.com/read/29337250/the-stim1-inhibitor-ml9-disrupts-basal-autophagy-in-cardiomyocytes-by-decreasing-lysosome-content
#14
S Shaikh, R Troncoso, D Mondaca-Ruff, V Parra, L Garcia, M Chiong, S Lavandero
Stromal-interaction molecule 1 (STIM1)-mediated store-operated Ca2+ entry (SOCE) plays a key role in mediating cardiomyocyte hypertrophy, both in vitro and in vivo. Moreover, there is growing support for the contribution of SOCE to the Ca2+ overload associated with ischemia/reperfusion injury. Therefore, STIM1 inhibition is proposed as a novel target for controlling both hypertrophy and ischemia/reperfusion-induced Ca2+ overload. Our aim was to evaluate the effect of ML9, a STIM1 inhibitor, on cardiomyocyte viability...
January 11, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29335300/extracellular-atp-activates-store-operated-ca2-entry-in-white-adipocytes-functional-evidence-for-stim1-and-orai1
#15
Mickaël F El Hachmane, Anna Ermund, Cecilia Brännmark, Charlotta S Olofsson
Here we have applied ratiometric measurements of intracellular Ca2+ concentrations ([Ca2+]i) to show that extracellularly applied ATP (100 mM) stimulates store-operated Ca2+ entry (SOCE) in 3T3-L1 adipocytes. ATP produced a rapid increase in [Ca2+]i consisting of an initial transient elevation followed by a sustained elevated phase that could be observed only in the presence of extracellular Ca2+ Gene expression data and [Ca2+]i recordings with uridine-5'-triphosphate (UTP) or with the phospholipase C (PLC) inhibitor U73122 demonstrated the involvement of purinergic P2Y2 receptors and the PLC/inositol trisphosphate (IP3) pathway...
January 15, 2018: Biochemical Journal
https://www.readbyqxmd.com/read/29326165/phosphorylation-mediated-structural-changes-within-the-soar-domain-of-stim1-enable-specific-activation-of-distinct-orai-channels
#16
Jill L Thompson, Yue Zhao, Peter B Stathopulos, Alan Grossfield, Trevor J Shuttleworth
The low-conductance, highly calciumselective channels formed by the Orai proteins are known to take two major forms, namely the storeoperated CRAC channels, and store-independent, arachidonic acid-activated ARC channels. Both are activated by STIM1, but whereas CRAC channels are activated by the stromal-interacting STIM1 located in the endoplasmic reticulum membrane, ARC channels are activated by the minor pool of STIM1 located in the plasma membrane. Critically, maximally activated CRAC channel currents and ARC channel currents are completely additive within the same cell, demonstrating that these two channels are entirely distinct entities...
January 11, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29324706/endothelial-ca2-signaling-and-the-resistance-to-anticancer-treatments-partners-in-crime
#17
REVIEW
Francesco Moccia
Intracellular Ca2+ signaling drives angiogenesis and vasculogenesis by stimulating proliferation, migration, and tube formation in both vascular endothelial cells and endothelial colony forming cells (ECFCs), which represent the only endothelial precursor truly belonging to the endothelial phenotype. In addition, local Ca2+ signals at the endoplasmic reticulum (ER)-mitochondria interface regulate endothelial cell fate by stimulating survival or apoptosis depending on the extent of the mitochondrial Ca2+ increase...
January 11, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29316690/vsp-17-a-new-ppar%C3%AE-agonist-suppresses-the-metastasis-of-triple-negative-breast-cancer-via-upregulating-the-expression-of-e-cadherin
#18
Yuhui Wang, Menglin Zhu, Bo Yuan, Kefeng Zhang, Mingli Zhong, Wei Yi, Xiaotian Xu, Xiaoqun Duan
Triple-negative breast cancer (TNBC), an aggressive subtype of breast cancer, shows higher metastases and relapse rates than other subtypes. The metastasis of TNBC is the main reason for the death of TNBC patients. Increasing evidence has shown that inhibiting the metastasis of TNBC is a good method for TNBC treatment. Here, VSP-17 was designed and synthesized as an agonist of PPARγ, evidenced by upregulating the expression of CD36 and increasing the activity of PPARγ reporter gene. VSP-17 obviously inhibited the migration and invasion process of MDA-MB-231 cells but showed little effect on the viability of MDA-MB-231 cells...
January 8, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29311116/stim1-activation-of-adenylyl-cyclase-6-connects-ca2-and-camp-signaling-during-melanogenesis
#19
Rajender K Motiani, Jyoti Tanwar, Desingu Ayyappa Raja, Ayushi Vashisht, Shivangi Khanna, Sachin Sharma, Sonali Srivastava, Sridhar Sivasubbu, Vivek T Natarajan, Rajesh S Gokhale
Endoplasmic reticulum (ER)-plasma membrane (PM) junctions form functionally active microdomains that connect intracellular and extracellular environments. While the key role of these interfaces in maintenance of intracellular Ca2+ levels has been uncovered in recent years, the functional significance of ER-PM junctions in non-excitable cells has remained unclear. Here, we show that the ER calcium sensor protein STIM1 (stromal interaction molecule 1) interacts with the plasma membrane-localized adenylyl cyclase 6 (ADCY6) to govern melanogenesis...
January 8, 2018: EMBO Journal
https://www.readbyqxmd.com/read/29307859/zn2-transporters-zip7-and-znt7-play-important-role-in-progression-of-cardiac-dysfunction-via-affecting-sarco-endo-plasmic-reticulum-mitochondria-coupling-in-hyperglycemic-cardiomyocytes
#20
Erkan Tuncay, Verda Bitirim, Yusuf Olgar, Aysegul Durak, Guy A Rutter, Belma Turan
Functional contribution of S(E)R-mitochondria coupling to normal cellular processes is crucial and any alteration in S(E)R-mitochondria axis may be responsible for the onset of diseases. Mitochondrial free Zn2+ level in cardiomyocytes ([Zn2+]Mit) is lower comparison to either its cytosolic or S(E)R level under physiological condition. However, there is little information about distribution of Zn2+-transporters on mitochondria and role of Zn2+-dependent mitochondrial-function associated with [Zn2+]Mit. Since we recently have shown how hyperglycemia (HG)-induced changes in ZIP7 and ZnT7 contribute to Zn2+-transport across S(E)R and contribute to S(E)R-stress in the heart, herein, we hypothesized that these transporters can also be localized to mitochondria and affect the S(E)R-mitochondria coupling, and thereby contribute to cellular Zn2+-muffling between S(E)R-mitochondria in HG-cells...
January 4, 2018: Mitochondrion
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