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CF-DNA

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https://www.readbyqxmd.com/read/28889199/oral-delivery-of-staphylococcal-nuclease-by-lactococcus-lactis-prevents-type-1-diabetes-mellitus-in-nod-mice
#1
Junchao Lang, Xiaoke Wang, Kunfeng Liu, Dongmei He, Pancong Niu, Rongyue Cao, Liang Jin, Jie Wu
Type 1 diabetes mellitus (T1DM) is an autoimmune disease characterized by the self-destruction of insulin-producing β cells. Recently, studies have revealed that neutrophils contribute to the early pathological injury to the pancreas, predominantly via the formation of neutrophil extracellular traps (NETs). To determine whether early intervention targeting NETs with staphylococcal nuclease (SNase) can delay the onset of T1DM, non-obese diabetic (NOD) mice were orally administered recombinant Lactococcus lactis (L...
September 9, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28625258/-plasma-level-of-neutrophil-extracellular-traps-in-septic-patients-and-its-clinical-significance-a-prospective-observational-study
#2
Fangxiao Zhang, Zhidan Zhang, Xiaochun Ma
OBJECTIVE: To investigate the changes of neutrophil extracellular traps (NETs) level in plasma of sepsis patients and judge its clinical value for early diagnosing of sepsis. METHODS: A prospective observational study was conducted. The patients after surgery aged > 18 years and expected to stay in the ICU > 24 hours admitted to intensive care unit (ICU) of the First Affiliated Hospital of China Medical University from November 2014 to February 2015 were enrolled...
February 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28623663/structural-mechanism-of-atp-independent-transcription-initiation-by-rna-polymerase-i
#3
Yan Han, Chunli Yan, Thi Hoang Duong Nguyen, Ashleigh J Jackobel, Ivaylo Ivanov, Bruce A Knutson, Yuan He
Transcription initiation by RNA Polymerase I (Pol I) depends on the Core Factor (CF) complex to recognize the upstream promoter and assemble into a Pre-Initiation Complex (PIC). Here, we solve a structure of Saccharomyces cerevisiae Pol I-CF-DNA to 3.8 Å resolution using single-particle cryo-electron microscopy. The structure reveals a bipartite architecture of Core Factor and its recognition of the promoter from -27 to -16. Core Factor's intrinsic mobility correlates well with different conformational states of the Pol I cleft, in addition to the stabilization of either Rrn7 N-terminal domain near Pol I wall or the tandem winged helix domain of A49 at a partially overlapping location...
June 17, 2017: ELife
https://www.readbyqxmd.com/read/28584706/impact-of-enzymatic-digestion-on-bacterial-community-composition-in-cf-airway-samples
#4
Kayla M Williamson, Brandie D Wagner, Charles E Robertson, Emily J Johnson, Edith T Zemanick, J Kirk Harris
BACKGROUND: Previous studies have demonstrated the importance of DNA extraction methods for molecular detection of Staphylococcus, an important bacterial group in cystic fibrosis (CF). We sought to evaluate the effect of enzymatic digestion (EnzD) prior to DNA extraction on bacterial communities identified in sputum and oropharyngeal swab (OP) samples from patients with CF. METHODS: DNA from 81 samples (39 sputum and 42 OP) collected from 63 patients with CF was extracted in duplicate with and without EnzD...
2017: PeerJ
https://www.readbyqxmd.com/read/28542490/repeated-bouts-of-exhaustive-exercise-increase-circulating-cell-free-nuclear-and-mitochondrial-dna-without-development-of-tolerance-in-healthy-men
#5
Robert Stawski, Konrad Walczak, Piotr Kosielski, Pawel Meissner, Tomasz Budlewski, Gianluca Padula, Dariusz Nowak
OBJECTIVE: Acute single strenuous exercise increases circulating cell free DNA (cf DNA). We tested whether three repeated bouts of exhaustive exercise induced the cf DNA response without development of tolerance in healthy men. METHODS: Eleven average-trained men (age 34.0±5.2 years, body mass index 26.2±3.1 kg/m2, maximal oxygen consumption-VO2max 49.6±4.5 ml/kg*min) performed three treadmill exercise tests to exhaustion at speed corresponding to 70% VO2max separated by 72 hours of resting...
2017: PloS One
https://www.readbyqxmd.com/read/28420468/-effect-of-heparin-pretreatment-on-the-level-of-neutrophil-extracellular-traps-of-serum-and-lung-tissue-in-septic-mice
#6
Jing Jiang, Shengtian Mu, Fangxiao Zhang, Yanting Qiao, Yongran Wu, Zhidan Zhang, Xiaochun Ma
OBJECTIVE: To investigate the influence of heparin pretreatment on serum and lung tissue level of neutrophil extracellular traps (NETs) in septic mice model and its molecular mechanism. METHODS: Ninety male C57BL/6J mice were randomly divided into control group (n = 30), lipopolysaccharides (LPS) group (n = 30, 30 mg/kg LPS in 100 ?L normal saline was intraperitoneally injected) and LPS+heparin group (n = 30, 8 U of heparin in 20 ?L normal saline was subcutaneously injected 30 minutes before the injection of LPS)...
April 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28397325/analysis-of-cell-free-dna-in-maternal-blood-in-screening-for-aneuploidies-updated-meta-analysis
#7
REVIEW
M M Gil, V Accurti, B Santacruz, M N Plana, K H Nicolaides
OBJECTIVES: To review clinical validation or implementation studies of maternal blood cell-free (cf) DNA analysis and define the performance of screening for fetal trisomies 21, 18 and 13 and sex chromosome aneuploidies (SCA). METHODS: Searches of PubMed, EMBASE and The Cochrane Library were performed to identify all peer-reviewed articles on cfDNA testing in screening for aneuploidies between January 2011, when the first such study was published, and 31 December 2016...
April 11, 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28374559/incorporating-thyroid-markers-in-down-syndrome-screening-protocols
#8
Ishraq Dhaifalah, Tomas Salek, Dagmar Langova, Howard Cuckle
OBJECTIVE: The article aimed to assess the benefit of incorporating maternal serum thyroid disease marker levels (thyroid-stimulating hormone and free thyroxine) into first trimester Down syndrome screening protocols. METHODS: Statistical modelling was used to predict performance with and without the thyroid markers. Two protocols were considered: the combined test and the contingent cell-free DNA (cfDNA) test, where 15-40% women are selected for cfDNA because of increased risk based on combined test results...
April 3, 2017: Prenatal Diagnosis
https://www.readbyqxmd.com/read/28365952/cell-free-dna-as-biomarker-and-source-for-mutation-detection-in-primary-colorectal-cancer
#9
Aleksandra Nikolic, Marina Vlajnic, Momcilo Ristanovic, Jelena Petrovic, Ivan Dimitrijevic, Zoran Krivokapic, Dragica Radojkovic
PURPOSE: To analyze if cell-free (cf)DNA levels and the presence of KRAS and BRAF mutations in serum could be used as diagnostic biomarkers in patients with primary colorectal cancer (CRC). METHODS: This study included 92 individuals who were operated due to primary CRC (N=52;study group) and to hemorrhoids (N=40;control group). Serum cfDNA levels were measured with real-time PCR (RT-PCR) using PicoGreen dsDNA quantitation reagent. Colorectal tissue and related blood and serum samples taken at the time of surgery were subjected to DNA extraction and analysis of KRAS and BRAF mutations based on multiplex SNaPshot assay and DNA sequencing...
January 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/28238813/genomewide-bisulfite-sequencing-reveals-the-origin-and-time-dependent-fragmentation-of-urinary-cfdna
#10
Timothy H T Cheng, Peiyong Jiang, Jacqueline C W Tam, Xiao Sun, Wing-Shan Lee, Stephanie C Y Yu, Jeremy Y C Teoh, Peter K F Chiu, Chi-Fai Ng, Kai-Ming Chow, Cheuk-Chun Szeto, K C Allen Chan, Rossa W K Chiu, Y M Dennis Lo
Urinary cell-free (cf) DNA holds great potential as a completely noninvasive form of liquid biopsy. Knowledge of the composition of cfDNA by tissue of origin is useful for guiding its clinical uses. We conducted a global survey of urinary cfDNA composition using genomewide bisulfite sequencing. While previous studies focused on detecting cfDNA from a single source at a time, genomewide tissue specific methylation signatures allow us to simultaneously deduce the proportional contribution from each contributing tissue...
June 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28186965/cell-free-dna-detected-by-liquid-biopsy-as-a-potential-prognostic-biomarker-in-early-breast-cancer
#11
Roberta Maltoni, Valentina Casadio, Sara Ravaioli, Flavia Foca, Maria Maddalena Tumedei, Samanta Salvi, Filippo Martignano, Daniele Calistri, Andrea Rocca, Alessio Schirone, Dino Amadori, Sara Bravaccini
As conventional biomarkers for defining breast cancer (BC) subtypes are not always capable of predicting prognosis, search for new biomarkers which can be easily detected by liquid biopsy is ongoing. It has long been known that cell-free DNA (CF-DNA) could be a promising diagnostic and prognostic marker in different tumor types, although its prognostic value in BC is yet to be confirmed. This retrospective study evaluated the prognostic role of CF-DNA quantity and integrity of HER2, MYC, BCAS1 and PI3KCA, which are frequently altered in BC...
March 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28124379/nuchal-translucency-measurement-in-the-era-of-prenatal-screening-for-aneuploidy-using-cell-free-cf-dna
#12
Barbara M O'Brien, Jacquelyn Halliday, Geralyn Lambert-Messerlian, Elizabeth E Eklund, Edward Kloza, Glenn E Palomaki
No abstract text is available yet for this article.
March 2017: Prenatal Diagnosis
https://www.readbyqxmd.com/read/27806655/screening-for-trisomies-21-and-18-in-a-spanish-public-hospital-from-the-combined-test-to-the-cell-free-dna-test
#13
M M Gil, M Brik, C Casanova, R Martin-Alonso, M Verdejo, E Ramírez, B Santacruz
OBJECTIVE: To describe our experience in first-trimester screening for trisomies 21 and 18 firstly by the combined test alone and secondly by cell-free (cf) DNA testing contingent on the results from a previously performed combined test. METHODS: Women with singleton pregnancies attending Torrejon University Hospital in Madrid, Spain, from November 2011 to January 2016, were screened for trisomy (T)21 and T18 by the combined test at 11-13 weeks. Before the introduction of cfDNA testing, women at high risk (>1 in 250) were offered invasive testing (IT) and from January 2015 they were offered cfDNA test as well as IT...
October 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/27793311/women-should-decide-which-conditions-matter
#14
EDITORIAL
Mary E Norton, Miriam Kuppermann
Providing reliable prenatal screening performance estimates is critical for patient counseling and policy-making. Women who choose prenatal screening for aneuploidy are likely to be concerned not only with the common aneuploidies but with all causes of intellectual disability and serious birth defects. Sequential prenatal screening (combined serum and ultrasound testing) for aneuploidy detection commonly is offered as a primary screening test. Among women identified as screen positive, cell-free (cf)DNA has been added recently as a secondary, noninvasive screening option, before the consideration of invasive diagnostic testing (eg, amniocentesis and karyotype)...
November 2016: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27793310/where-have-all-the-trisomies-gone
#15
EDITORIAL
Glenn E Palomaki, Geralyn M Lambert-Messerlian, James E Haddow
Providing reliable prenatal screening performance estimates is critical for patient counseling and policy-making. Women who choose prenatal screening for aneuploidy are likely to be concerned not only with the common aneuploidies but with all causes of intellectual disability and serious birth defects. Sequential prenatal screening (combined serum and ultrasound testing) for aneuploidy detection commonly is offered as a primary screening test. Among women identified as screen positive, cell-free (cf)DNA has been added recently as a secondary, noninvasive screening option, before the consideration of invasive diagnostic testing (eg, amniocentesis and karyotype)...
November 2016: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27687626/a-study-of-tumor-heterogeneity-in-a-case-with-breast-cancer
#16
Haruka Nakada, Hiroshi Nakagomi, Yosuke Hirotsu, Kenji Amemiya, Hitoshi Mochizuki, Masayuki Inoue, Toshio Oyama, Masao Omata
Tumor heterogeneity has been suggested based on clinical and pathological findings. Several clinical findings can be explained by tumor evolution during progression and metastasis. We herein report a case of metastatic breast cancer indicated tumor heterogeneity by clinical findings and a genomic analysis. A 64-year-old woman with advanced breast cancer was treated with primary chemotherapy, to which primary tumor responded. After a 6 month treatment pause, lung, liver, and skin metastases developed and her serum tumor markers were elevated...
May 2017: Breast Cancer: the Journal of the Japanese Breast Cancer Society
https://www.readbyqxmd.com/read/27646960/ep09-03-prenatal-cf-dna-testing-for-down-s-syndrome-and-other-r-chromosomal-aneuploidies-clinical-outcome-in-private-and-public-health-systems
#17
D M Strah, F Mujezinović
No abstract text is available yet for this article.
September 2016: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/27634416/quantitative-analysis-of-plasma-cell-free-dna-and-its-dna-integrity-in-patients-with-metastatic-prostate-cancer-using-alu-sequence
#18
Amal Fawzy, Karima M Sweify, Hany M El-Fayoumy, Nagwa Nofal
BACKGROUND: Prostate cancer (PC) is the most common cancer affecting men, it accounts for 29% of all male cancer and 11% of all male cancer related death. DNA is normally released from an apoptotic source which generates small fragments of cell-free DNA, whereas cancer patients have cell-free circulating DNA that originated from necrosis, autophagy, or mitotic catastrophe, which produce large fragments. AIM OF WORK: Differentiate the cell free DNA levels (cfDNA) and its integrity in prostate cancer patients and control group composed of benign prostate hyperplasia (BPH) and healthy persons...
December 2016: Journal of the Egyptian National Cancer Institute
https://www.readbyqxmd.com/read/27584908/accuracy-and-clinical-value-of-maternal-incidental-findings-during-noninvasive-prenatal-testing-for-fetal-aneuploidies
#19
Nathalie Brison, Kris Van Den Bogaert, Luc Dehaspe, Jessica M E van den Oever, Katrien Janssens, Bettina Blaumeiser, Hilde Peeters, Hilde Van Esch, Griet Van Buggenhout, Annick Vogels, Thomy de Ravel, Eric Legius, Koen Devriendt, Joris R Vermeesch
PURPOSE: Genome-wide sequencing of cell-free (cf)DNA of pregnant women aims to detect fetal chromosomal imbalances. Because the largest fraction of cfDNA consists of maternal rather than fetal DNA fragments, maternally derived copy-number variants (CNVs) are also measured. Despite their potential clinical relevance, current analyses do not interpret maternal CNVs. Here, we explore the accuracy and clinical value of maternal CNV analysis. METHODS: Noninvasive prenatal testing was performed by whole-genome shotgun sequencing on plasma samples...
September 1, 2016: Genetics in Medicine: Official Journal of the American College of Medical Genetics
https://www.readbyqxmd.com/read/27509651/-clinical-implementation-of-cell-free-dna-analysis-in-maternal-blood-in-screening-for-aneuploidies-in-singleton-pregnancies
#20
P Chaveeva, M Yankova, V Stratieva, I Dimitrov, A Shterev
OBJECTIVE: Clinical implementation of cell free(cf) DNA testing in maternal blood for aneuploidies in singleton pregnancies. METHODS: This is a retrospective study conducted in two centers for fetal medicine in Sofia, Bulgaria, between October 2013 and August 2015. We examined the clinical implementation of cf DNA testing in the routine practice for trisomies 21, 18 and13 after the performance of the first trimester combined test, second trimester biochemical test and/or the combination between first and second trimester integrated test...
2016: Akusherstvo i Ginekologii︠a︡
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