Read by QxMD icon Read


Di Huang, Lulu Niu, Jian Li, Jingjing Du, Yan Wei, Yinchun Hu, Xiaojie Lian, Weiyi Chen, Kaiqun Wang
In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane. The morphologies and mechanical properties of the composite membranes were investigated through scanning electronic microscopy (SEM) and universal mechanical testing machine. The results show that the in situ biomimetic nHA/CS microspheres were embedded in CS membrane and were integrated tightly with CS matrix...
March 7, 2018: Journal of the Mechanical Behavior of Biomedical Materials
Bo Yin, Bingjian Xue, Zhihong Wu, Jiguang Ma, Keming Wang
The aim of this study was to explore an innovative method to improve the osteogenic ability of porous titanium. We used gelatin (Gel) and nano-hydroxyapatite (nHA) to construct micro-scaffolds within the pores of porous titanium alloy. We compared three groups: control, Gel:nHA = 1:0, and Gel:nHA = 1:1. We assessed cell attachment, cell proliferation, and osteogenic (alkaline phosphatase [ALP] and collagen type 1 [Col-1]) and cytoskeletal (Talin) gene and protein expression in MC3T3-E1 cells. We also evaluated osteogenic abilities in a rabbit calvarial defect model...
2018: American Journal of Translational Research
Yiying Sun, Xiaoyan Lu, Yunyun Gai, Chunjie Sha, Guangyi Leng, Xiucheng Yang, Wanhui Liu
A sensitive and selective LC-MS/MS method for determination of the prodrug aripiprazole lauroxil (AL) and the three metabolites (N-hydroxymethyl aripiprazole [NHA], aripiprazole [ARP], and dehydro aripiprazole [DHA]) in plasma was developed using ARP-d8 as an internal standard. The analytes were determined on an AB Sciex Triple Quad™ 4500 system using positive ion electrospray ionization and selected multiple reaction monitoring mode. Solid phase extraction was applied for sample preparation for AL, ARP, and DHA, and protein precipitation for NHA...
February 10, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Behzad Shiroud Heidari, Seyed Mohammad Davachi, Amin Hedayati Moghaddam, Javad Seyfi, Iman Hejazi, Razi Sahraeian, Hamid Rashedi
In this study, injection molding process of ultrahigh molecular weight polyethylene (UHMWPE) reinforced with nano-hydroxyapatite (nHA) was simulated and optimized through minimizing the shrinkage and warpage of the hip liners as an essential part of a hip prosthesis. Fractional factorial design (FFD) was applied to the design of the experiment, modeling, and optimizing the shrinkage and warpage of UHMWPE/nHA composite liners. The Analysis of variance (ANOVA) was applied to find the importance of operative parameters and their effects...
February 21, 2018: Journal of the Mechanical Behavior of Biomedical Materials
Povilas Daugela, Mindaugas Pranskunas, Gintaras Juodzbalys, Jolanta Liesiene, Odeta Baniukaitiene, Américo Afonso, Pedro de Sousa Gomes
Cellulose scaffolds containing nano- or micro-hydroxyapatite (nHA or μHA) were prepared by the regeneration of cellulose from its acetylated derivative and the mechanical immobilization of inorganic particles, followed by freeze-drying. Microtomographic (μCT) evaluation revealed that both scaffolds presented a highly interconnected porous structure, with a mean pore diameter of 490±94 μm and 540±132 μm for cellulose/nHA and cellulose/μHA, respectively. In vitro and in vivo characterizations of the developed scaffolds were investigated...
March 2, 2018: Journal of Tissue Engineering and Regenerative Medicine
Anne-Sophie Ducloy-Bouthors, Emmanuelle Jeanpierre, Imen Saidi, Anne-Sophie Baptiste, Elodie Simon, Damien Lannoy, Alain Duhamel, Delphine Allorge, Sophie Susen, Benjamin Hennart
BACKGROUND: Evidence increases that a high or a standard dose of tranexamic acid (TA) reduces postpartum bleeding. The TRACES pharmacobiological substudy aims to establish a therapeutic strategy in hemorrhagic (H) Cesarean section (CS) with respect to the intensity of fibrinolysis by using innovative assays. METHOD/DESIGN: The TRACES trial is a multicenter, randomized, double-blind, placebo-controlled, TA dose-ranging study that measures simultaneously plasmatic and uterine and urine TA concentrations and the plasmin peak inhibition tested by a simultaneous thrombin plasmin generation assay described by Van Geffen (novel hemostasis assay [NHA])...
March 1, 2018: Trials
Irene Mencía Castaño, Caroline M Curtin, Garry Paul Duffy, Fergal J O'Brien
MicroRNA (miRNA) therapeutics are increasingly being developed to either target bone-related diseases such as osteoporosis and osteoarthritis or as the basis for novel bone tissue engineering strategies. A number of miRNAs have been reported as potential osteo-therapeutics but no consensus has yet been established on the optimal target. miR-16 has been studied extensively in non-osteogenic functions and used as functionality reporter target in the development of non-viral microRNA delivery platforms. This study hypothesised that miR-16 may also play an inhibitory role in osteogenesis due to its ability to directly target Smad5 & AcvR2a...
February 28, 2018: Tissue Engineering. Part A
Zhiming Ma
BACKGROUND: SETD8 (named PR-SET7 or KMT5a) has been reported to regulate various biological processes including carcinogenesis. However, the role of SETD8 in glioma progression has not been investigated. METHOD: qPCR and western blot were used to detect the expression levels of miR-382 and SETD8. MTT and wound healing assay used to detect the cell proliferation and migratory capability. A predicted target of miR-382 (SETD8) was first validated using a luciferase assay...
January 31, 2018: Pathology, Research and Practice
Sahar Elkholy, Sarah Yahia, Manal Awad, Medhat Elmessiery
BACKGROUND: Natural polymer composite materials are becoming increasingly important as scaffolds for bone tissue engineering. Composite materials based on combinations of biodegradable polymers and bioactive ceramics, including CTS and Hap. PURPOSE: β-Chitosan/n-HA composite with different percentages was prepared. Some of the physical and mechanical properties were examined by using (scanning electron microscope and transmission electron microscope). Histological evaluation of in vivo implantation of β-Chitosan/n-HA composite as bone graft material was done...
February 26, 2018: Clinical Implant Dentistry and related Research
Bipin Gaihre, Suren Uswatta, Ambalangodage C Jayasuriya
In this study, injectable porous spherical particles were fabricated using chitosan (CS) biopolymer, sodium tripolyphosphate (TPP), and nano-hydroxyapatite (nHA). TPP was primarily used as an ionic crosslinker to crosslink 2% (w/v) CS droplets. 2% (w/v) nHA was used to prepare nHA incorporated particles. The surface morphological properties and nanomechanical properties such as topography, deformation, adhesion, and dissipation of CS particles with and without nHA were studied using contact mode and peakforce quantitative nanomechanical property mapping mode in atomic force microscopy...
February 15, 2018: Colloids and Surfaces. B, Biointerfaces
Xing Yang, Yuanyuan Li, Qianli Huang, Xujie Liu, Ranran Zhang, Qingling Feng
Due to its excellent biocompatibility, nanosized hydroxyapatite (nHA) has drawn much attention for various applications in biomedical fields. There are growing concerns about its biosecurity, however, little is known about its effects on adipogenesis. In the present study, nHA with three different sizes were synthesized and the in vitro effects of nHA on cell proliferation and adipogenic differentiation of human mesenchymal stem cells (hMSCs) were investigated. The results clearly show that nHA does not affect the cell viability, the lipids droplets formation, triglyceride (TG) synthesis and the expression of adipogenic marker genes/proteins of hMSCs at the concentrations lower than 50 μg/ml...
February 22, 2018: Journal of Biomedical Materials Research. Part A
Quan Nha Hong, Araceli Gonzalez-Reyes, Pierre Pluye
RATIONALE, AIMS, AND OBJECTIVES: Systematic reviews combining qualitative, quantitative, and/or mixed methods studies are increasingly popular because of their potential for addressing complex interventions and phenomena, specifically for assessing and improving clinical practice. A major challenge encountered with this type of review is the appraisal of the quality of individual studies given the heterogeneity of the study designs. The Mixed Methods Appraisal Tool (MMAT) was developed to help overcome this challenge...
February 21, 2018: Journal of Evaluation in Clinical Practice
Hadi Samadian, Majid Salehi, Saeed Farzamfar, Ahmad Vaez, Arian Ehterami, Hamed Sahrapeyma, Arash Goodarzi, Sadegh Ghorbani
The present study aimed to evaluate the efficacy of cellulose acetate/gelatin/nanohydroxyapatite (CA/Gel/nHA) nanocomposite mats as the wound dressing. The dressings were prepared with electrospinning of CA/Gel solutions containing 12.5, 25 and 50 mg nHA. The dressings were evaluated regarding their water uptake capacity, morphology, tensile strength, water vapour transmission rate, wettability and cellular response with L929 cell line. The results showed that the concentration of nHA had a direct correlation with porosity, water contact angle, water uptake, water vapor transmission rate and proliferation...
February 19, 2018: Artificial Cells, Nanomedicine, and Biotechnology
C Curtin, J C Nolan, R Conlon, L Deneweth, C Gallagher, Y J Tan, B L Cavanagh, A Z Asraf, H Harvey, S Miller-Delaney, J Shohet, I Bray, F J O'Brien, R L Stallings, O Piskareva
3D scaffold-based in vitro cell culturing is a recent technological advancement in cancer research bridging the gap between conventional 2D culture and in vivo tumours. The main challenge in treating neuroblastoma, a paediatric cancer of the sympathetic nervous system, is to combat tumour metastasis and resistance to multiple chemotherapeutic drugs. The aim of this study was to establish a physiologically relevant 3D neuroblastoma tissue-engineered system and explore its therapeutic relevance. Two neuroblastoma cell lines, chemotherapeutic sensitive Kelly and chemotherapeutic resistant KellyCis83 were cultured in a 3D in vitro model on two collagen-based scaffolds containing either glycosaminoglycan (Coll-GAG) or nanohydroxyapatite (Coll-nHA) and compared to 2D cell culture and an orthotopic murine model...
February 12, 2018: Acta Biomaterialia
Janani Radhakrishnan, Amrutha Manigandan, Prabu Chinnaswamy, Anuradha Subramanian, Swaminathan Sethuraman
Fabrication of anisotropic osteochondral-mimetic scaffold with mineralized subchondral zone and gradient interface remains challenging. We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively. Mineralized subchondral hydrogel exhibited significantly higher osteoblast proliferation and alkaline phosphatase activity (p < 0.05). Osteochondral hydrogel exhibited interconnected porous structure and spatial variation with gradient interface of nHA and ChS-NPs...
February 3, 2018: Biomaterials
Maryam Shahi, Mahmood Nadari, Mehdi Sahmani, Ehsan Seyedjafari, Naser Ahmadbeigi, Amir Peymani
The limitation of traditional bone grafts could be overcome by applying engineered bone constructs, which are mainly produced by seeding suitable stem cells on appropriate scaffolds. So far, bone marrow-derived stromal cells have been the most applied cells in bone tissue engineering, but current data show that unrestricted somatic stem cells (USSCs) from human cord blood might actually be a better stem cell source due to the accessibility and noninvasive procedure of collection. In this study, we cultured USSCs on a plasma-treated electrospun polylactic-co-glycolic acid (PLGA) scaffold coated with nanohydroxyapatite (nHA)...
February 8, 2018: Cells, Tissues, Organs
Horieh Moosavi, Narjes Hakimi
Background: This study investigated the effect of post bleaching treatments to the change of enamel elastic modulus and microhardness after dental bleaching in- vitro. Material and Methods: Fifty bovine incisor slab were randomly assigned into five groups (n=10). The samples were bleached for three times; 20 minutes each time, by 40% hydrogen peroxide. Next it was applied fractional CO2 laser for two minutes, Nano- hydroxy apatite (N-HA) and MI-paste for 7 days and 2 minutes per day...
December 2017: Journal of Clinical and Experimental Dentistry
Igor Shamovsky, Graham Belfield, Richard Lewis, Frank Narjes, Lena Ripa, Christian Tyrchan, Lisa Öberg, Peter Sjö
Nitric oxide (NO·) is a messenger molecule with diverse physiological roles including host defense, neurotransmission and vascular function. The synthesis of NO· from l-arginine is catalyzed by NO-synthases and occurs in two steps through the intermediary Nω-hydroxy-l-arginine (NHA). In both steps the P450-like reaction cycle is coupled with the redox cycle of the cofactor tetrahydrobiopterin (H4B). The mechanism of the second step is studied by Density Functional Theory calculations to ascertain the canonical sequence of proton and electron transfer (PT and ET) events...
January 10, 2018: Journal of Inorganic Biochemistry
Gerald Moncayo, Michal Grzmil, Tatiana Smirnova, Pawel Zmarz, Roland M Huber, Debby Hynx, Hubertus Kohler, Yuhua Wang, Hans-Rudolf Hotz, Nancy E Hynes, Georg Keller, Stephan Frank, Adrian Merlo, Brian A Hemmings
Background: Glioblastoma (GBM) is one of the most aggressive human brain tumors with a median survival of 15-18 months. There is a desperate need to find novel therapeutic targets. Various receptor protein kinases have been identified as potential targets; however, response rates in clinical studies have been somewhat disappointing. Targeting the Spleen Tyrosine Kinase (SYK), which acts downstream of a range of oncogenic receptors, may therefore show more promising results. Methods: Kinase expression of brain tumor samples including GBM and low-grade tumors were compared to normal brain and normal human astrocytes (NHA) by microarray analysis...
February 1, 2018: Neuro-oncology
Minh-Nha Rhylie Nguyen, Cassandra Mosel, Luke E Grzeskowiak
Background: Medication errors represent a significant but often preventable cause of morbidity and mortality in neonates. The objective of this systematic review was to determine the effectiveness of interventions to reduce neonatal medication errors. Methods: A systematic review was undertaken of all comparative and noncomparative studies published in any language, identified from searches of PubMed and EMBASE and reference-list checking. Eligible studies were those investigating the impact of any medication safety interventions aimed at reducing medication errors in neonates in the hospital setting...
February 2018: Therapeutic Advances in Drug Safety
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"