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https://www.readbyqxmd.com/read/29454291/de-novo-transcriptome-of-the-pallial-gland-of-the-date-mussel-lithophaga-lithophaga
#1
Urška Sivka, Nataša Toplak, Simon Koren, Jernej Jakše
Lithophaga lithophaga is a rock-boring bivalve with an ability to dissolve carbonate substrata with its siphonal and pallial gland secretions. However, the molecular mechanism that enables this species to bore into calcareous rocks is not yet known. In order to identify genes potentially involved in chemical boring we performed transcriptome sequencing of pallial-gland tissue samples of L. lithophaga. Transcriptome sequencing using an Ion Torrent platform generated 60.563 million clean reads with an average read length of 96 bp...
February 6, 2018: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/29452641/cdc48-vcp-promotes-chromosome-morphogenesis-by-releasing-condensin-from-self-entrapment-in-chromatin
#2
Yogitha Thattikota, Sylvain Tollis, Roger Palou, Justine Vinet, Mike Tyers, Damien D'Amours
The morphological transformation of amorphous chromatin into distinct chromosomes is a hallmark of mitosis. To achieve this, chromatin must be compacted and remodeled by a ring-shaped enzyme complex known as condensin. However, the mechanistic basis underpinning condensin's role in chromosome remodeling has remained elusive. Here we show that condensin has a strong tendency to trap itself in its own reaction product during chromatin compaction and yet is capable of interacting with chromatin in a highly dynamic manner in vivo...
February 15, 2018: Molecular Cell
https://www.readbyqxmd.com/read/29452613/the-role-of-plasma-membrane-calcium-atpases-pmcas-in-neurodegenerative-disorders
#3
REVIEW
Parvana Hajieva, Marius W Baeken, Bernd Moosmann
Selective degeneration of differentiated neurons in the brain is the unifying feature of neurodegenerative disorders such as Parkinson's disease (PD) or Alzheimer's disease (AD). A broad spectrum of evidence indicates that initially subtle, but temporally early calcium dysregulation may be central to the selective neuronal vulnerability observed in these slowly progressing, chronic disorders. Moreover, it has long been evident that excitotoxicity and its major toxic effector mechanism, neuronal calcium overload, play a decisive role in the propagation of secondary neuronal death after acute brain injury from trauma or ischemia...
January 10, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29452610/the-plasma-membrane-calcium-pumps-the-old-and-the-new
#4
REVIEW
Asma Zaidi, Mercy Adewale, Lauren McLean, Paul Ramlow
The plasma membrane Ca 2+ -ATPase (PMCA) pumps play a critical role in the maintenance of calcium (Ca 2+ ) homeostasis, crucial for optimal neuronal function and cell survival. Loss of Ca 2+ homeostasis is a key precursor in neuronal dysfunction associated with brain aging and in the pathogenesis of neurodegenerative disorders. In this article, we review evidence showing age-related changes in the PMCAs in synaptic plasma membranes (SPMs) and lipid raft microdomains isolated from rat brain. Both PMCA activity and protein levels decline progressively with increasing age...
January 10, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29452608/insights-into-an-ancient-atypical-kinase-essential-for-biosynthesis-of-coenzyme-q
#5
Catherine F Clarke
COQ8 proteins are homologs of atypical protein kinases required for the biosynthesis of coenzyme Q (CoQ). In this issue of Cell Chemical Biology, Reidenbach et al. (2018) show that COQ8 has an ATPase activity, required for CoQ biosynthesis, that is strongly activated by cardiolipin and small molecule mimics of early CoQ intermediates.
February 15, 2018: Cell Chemical Biology
https://www.readbyqxmd.com/read/29450407/changes-in-the-cardiac-ghsr1a-ghrelin-system-correlate-with-myocardial-dysfunction-in-diabetic-cardiomyopathy-in-mice
#6
Rebecca Sullivan, Rebecca McGirr, Shirley Hu, Alice Tan, Derek Wu, Carlie Charron, Tyler Lalonde, Edith Arany, Subrata Chakrabarti, Leonard Luyt, Savita Dhanvantari
Ghrelin and its receptor, the growth hormone secretagogue receptor 1a (GHSR1a), are present in cardiac tissue. Activation of GHSR1a by ghrelin promotes cardiomyocyte contractility and survival, and changes in myocardial GHSR1a and circulating ghrelin track with end-stage heart failure, leading to the hypothesis that GHSR1a is a biomarker for heart failure. We hypothesized that GHSR1a could also be a biomarker for diabetic cardiomyopathy (DCM). We used two models of streptozotocin (STZ)-induced DCM: group 1, adult mice treated with 35 mg/kg STZ for 3 days; and group 2, neonatal mice treated with 70 mg/kg STZ at days 2 and 5 after birth...
February 1, 2018: Journal of the Endocrine Society
https://www.readbyqxmd.com/read/29449070/physiological-effects-of-marine-natural-organic-matter-and-metals-in-early-life-stages-of-the-north-pacific-native-marine-mussel-mytilus-trossulus-a-comparison-with-the-invasive-mytilus-galloprovincialis
#7
Lygia Sega Nogueira, Adalto Bianchini, Scott Smith, Marianna Basso Jorge, Rachael L Diamond, Chris M Wood
The role of seawater NOM in reducing metal toxicity for marine organisms is not well understood. We investigated the effects of five different marine NOMs (two autochthonous, one allochthonous, two of mixed origin, at 8 mg C/L), three metals (6 μg Cu/L; 20 μg Pb/L; 25 μg Zn/L), and combinations between them, to early life stages of Mytilus trossulus (a North Pacific native) in 48-h tests. Endpoints were whole body Ca 2+ +Mg 2+ -ATPase activity, carbonic anhydrase (CA) activity and lipid peroxidation. Comparisons were made with previously reported tests (identical conditions) on the invasive M...
December 19, 2017: Marine Environmental Research
https://www.readbyqxmd.com/read/29448933/the-curious-case-of-vacuolar-atpase-regulation-of-signaling-pathways
#8
REVIEW
Sahithi Pamarthy, Arpita Kulshrestha, Gajendra K Katara, Kenneth D Beaman
The Vacuolar ATPase (V-ATPase) is a proton pump responsible for controlling the intracellular and extracellular pH of cells. The structure of V-ATPase has been highly conserved among all eukaryotic cells and is involved in diverse functions across species. V-ATPase is best known for its acidification of endosomes and lysosomes and is also important for luminal acidification of specialized cells. Several reports have suggested the involvement of V-ATPase in maintaining an alkaline intracellular and acidic extracellular pH thereby aiding in proliferation and metastasis of cancer cells respectively...
February 15, 2018: Molecular Cancer
https://www.readbyqxmd.com/read/29444861/interacting-heads-motif-has-been-conserved-as-a-mechanism-of-myosin-ii-inhibition-since-before-the-origin-of-animals
#9
Kyoung Hwan Lee, Guidenn Sulbarán, Shixin Yang, Ji Young Mun, Lorenzo Alamo, Antonio Pinto, Osamu Sato, Mitsuo Ikebe, Xiong Liu, Edward D Korn, Floyd Sarsoza, Sanford I Bernstein, Raúl Padrón, Roger Craig
Electron microscope studies have shown that the switched-off state of myosin II in muscle involves intramolecular interaction between the two heads of myosin and between one head and the tail. The interaction, seen in both myosin filaments and isolated molecules, inhibits activity by blocking actin-binding and ATPase sites on myosin. This interacting-heads motif is highly conserved, occurring in invertebrates and vertebrates, in striated, smooth, and nonmuscle myosin IIs, and in myosins regulated by both Ca 2+ binding and regulatory light-chain phosphorylation...
February 14, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29444826/polymerase-%C3%AE-helicase-efficiently-unwinds-dna-and-rna-dna-hybrids
#10
Ahmet Yunus Ozdemir, Timur Rusanov, Tatiana Kent, Labiba A Siddique, Richard T Pomerantz
POLQ is a unique multifunctional replication and repair gene that encodes for a N-terminal superfamily 2 helicase and a C-terminal A-family polymerase. Although the function of the polymerase domain has been investigated, little is understood regarding the helicase domain. Multiple studies have reported that Polq-helicase is unable to unwind DNA. However, it exhibits ATPase activity that is stimulated by single-strand DNA which presents a biochemical conundrum. In contrast to previous reports, we demonstrate that Polq-helicase (residues 1-894) efficiently unwinds DNA with 3'-5' polarity, including DNA with 3' or 5' overhangs, blunt-ended DNA, and replication forks...
February 14, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29440755/neuropathic-morc2-mutations-perturb-ghkl-atpase-dimerization-dynamics-and-epigenetic-silencing-by-multiple-structural-mechanisms
#11
Christopher H Douse, Stuart Bloor, Yangci Liu, Maria Shamin, Iva A Tchasovnikarova, Richard T Timms, Paul J Lehner, Yorgo Modis
Missense mutations in MORC2 cause neuropathies including spinal muscular atrophy and Charcot-Marie-Tooth disease. We recently identified MORC2 as an effector of epigenetic silencing by the human silencing hub (HUSH). Here we report the biochemical and cellular activities of MORC2 variants, alongside crystal structures of wild-type and neuropathic forms of a human MORC2 fragment comprising the GHKL-type ATPase module and CW-type zinc finger. This fragment dimerizes upon binding ATP and contains a hinged, functionally critical coiled-coil insertion absent in other GHKL ATPases...
February 13, 2018: Nature Communications
https://www.readbyqxmd.com/read/29440177/ponatinib-shows-potent-antitumor-activity-in-small-cell-carcinoma-of-the-ovary-hypercalcemic-type-sccoht-through-multi-kinase-inhibition
#12
Jessica D Lang, William P D Hendricks, Krystal A Orlando, Hongwei Yin, Jeffrey Kiefer, Pilar Ramos, Ritin Sharma, Patrick Pirrotte, Elizabeth A Raupach, Chris Sereduk, Nanyun Tang, Winnie S Liang, Megan Washington, Salvatore J Facista, Victoria L Zismann, Emily M Cousins, Michael B Major, Yemin Wang, Anthony N Karnezis, Aleksandar Sekulic, Ralf Hass, Barbara C Vanderhyden, Praveen Nair, Bernard E Weissman, David G Huntsman, Jeffrey M Trent
PURPOSE: Small cell carcinoma of the ovary, hypercalcemic type (SCCOHT) is a rare, aggressive ovarian cancer in young women that is universally driven by loss of the SWI/SNF ATPase subunits SMARCA4 and SMARCA2. A great need exists for effective targeted therapies for SCCOHT. EXPERIMENTAL DESIGN: To identify underlying therapeutic vulnerabilities in SCCOHT, we conducted high-throughput siRNA and drug screens. Complementary proteomics approaches profiled kinases inhibited by ponatinib...
February 9, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29439719/myl6b-a-myosin-light-chain-promotes-mdm2-mediated-p53-degradation-and-drives-hcc-development
#13
Xingwang Xie, Xueyan Wang, Weijia Liao, Ran Fei, Nan Wu, Xu Cong, Qian Chen, Lai Wei, Yu Wang, Hongsong Chen
BACKGROUND: Identification of novel MDM2 or p53 binding proteins may reveal undefined oncogenes, tumor suppressors, signaling pathways and possible treatment targets. METHODS: By means of immunoprecipitation and Mass Spectrometry analysis, we aimed to identify novel regulators of the MDM2-p53 pathway. We further clarified the impact of MYL6B on the p53 protein level and on the process of apoptosis. We also investigated the role of MYL6B in hepatocellular carcinoma by clone formation assay and by determining the correlation between its expression and prognosis of HCC patients...
February 13, 2018: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29439211/comparative-analysis-of-arabidopsis-ecotypes-reveals-a-role-for-brassinosteroids-in-root-hydrotropism
#14
Rui Miao, Meng Wang, Wei Yuan, Yan Ren, Ying Li, Na Zhang, Jianhua Zhang, Herbert J Kronzucker, Weifeng Xu
Plant root responds to soil moisture gradients through hydrotropism and remodels root growth orientation chasing soil water, a process regarded as plant acclimation to a changing soil environment. Mechanisms underlying the root hydrotropic response, however, remain poorly understood. Here, hydrotropism was examined in 31 Arabidopsis ecotypes collected from different parts of the world and grown along moisture gradients in a specially designed soil-simulation system. More detailed comparative transcriptomic, ionomic profiling, and physiological analyses were carried out on three wild types, Ws (strongly hydrotropic), Col-0 (moderately hydrotropic), and C24 (weakly hydrotropic), and also in mutant lines showing disruptions in the root hydrotropic response...
February 8, 2018: Plant Physiology
https://www.readbyqxmd.com/read/29438630/isoform-specific-role-of-na-k-atpase-%C3%AE-1-in-skeletal-muscle
#15
Laura C Kutz, Shreya T Mukherji, Xiaoliang Wang, Amber Bryant, Isabel Larre, Judith A Heiny, Jerry B Lingrel, Sandrine V Pierre, Zijian Xie
The distribution of Na/K-ATPase α isoforms in skeletal muscle is unique, with α1 as the minor (15%) isoform and α2 comprising the bulk of the Na/K-ATPase pool. The acute and isoform-specific role of α2 in muscle performance and resistance to fatigue is well known, but the isoform-specific role of α1 has not been as thoroughly investigated. In vitro, we reported that α1 has a role in promoting cell growth that is not supported by α2. To assess whether α1 serves this isoform-specific trophic role in the skeletal muscle, we used the Na/K-ATPase α1 haploinsufficient (α1+/-) mice...
February 13, 2018: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29438067/functional-role-of-highly-conserved-residues-of-the-n-terminal-tail-and-first-transmembrane-segment-of-a-p4-atpase
#16
Rubén Perandrés-López, María P Sánchez-Cañete, Francisco Gamarro, Santiago Castanys
The P4 family of P-type ATPases (P4-ATPases) plays an important role in maintaining phospholipid asymmetry in eukaryotic cell membranes. Leishmania miltefosine transporter (LMT) is a plasma membrane (PM) P4-ATPase that catalyses translocation into the parasite of the leishmanicidal drug miltefosine as well as phosphatidylcholine and phosphatidylethanolamine analogues. In this study, we analysed the role, in LMT, of a series of highly-conserved amino acids previously non-described in the N-terminal region of P4-ATPases...
February 7, 2018: Biochemical Journal
https://www.readbyqxmd.com/read/29436780/autosomal-tubulointerstitial-kidney-disease-muc1-type-differential-proteomics-suggests-that-mutated-muc1-insc-affects-vesicular-transport-in-renal-epithelial-cells
#17
Simon Staubach, Andrea Wenzel, Bodo B Beck, Markus M Rinschen, Stefan Müller, Franz-Georg Hanisch
Autosomal dominant tubulointerstitial kidney disease associated to the MUC1 gene (ADTKD-MUC1; formerly MCKD1) belongs to a heterogenous group of rare hereditary kidney diseases that is prototypically caused by frameshift mutations in the MUC1 repeat domain. The mutant MUC1(insC) lacks the transmembrane domaine, exhibits aberant cellular topology and hence might gain a function during the pathological process. To get insight into potential pathomechanisms we performed differential proteomics of extracellular vesicles shed by renal epithelia into the urine of patients...
February 13, 2018: Proteomics
https://www.readbyqxmd.com/read/29436645/ouabain-suppresses-the-growth-and-migration-abilities-of-glioma-u%C3%A2-87mg-cells-through-inhibiting-the-akt-mtor-signaling-pathway-and-downregulating-the-expression-of-hif%C3%A2-1%C3%AE
#18
Xiao-Sa Yang, Zhong-Wei Xu, Tai-Long Yi, Rui-Cheng Xu, Jie Li, Wen-Bin Zhang, Sai Zhang, Hong-Tao Sun, Ze-Qi Yu, Hao-Xiang Xu, Yue Tu, Shi-Xiang Cheng
Glioma is one of the most malignant forms of brain tumor, and has been of persistent concern due to its high recurrence and mortality rates, and limited therapeutic options. As a cardiac glycoside, ouabain has widespread applications in congestive heart diseases due to its positive cardiac inotropic effect by inhibiting Na+/K+‑ATPase. Previous studies have demonstrated that ouabain has antitumor activity in several types of human tumor, including glioma. However, the exact underlying mechanism remains to be elucidated...
February 12, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29435724/transcriptome-expression-and-activity-analyses-reveal-a-vital-heat-shock-protein-70-in-the-stress-response-of-stony-coral-pocillopora-damicornis
#19
Yidan Zhang, Zhi Zhou, Lingui Wang, Bo Huang
Coral bleaching occurs worldwide with increasing frequencies and intensities, which is caused by the stress response of stony coral to environmental change, especially increased sea surface temperature. In the present study, transcriptome, expression, and activity analyses were employed to illustrate the underlying molecular mechanisms of heat shock protein 70 (HSP70) in the stress response of coral to environmental changes. The domain analyses of assembled transcripts revealed 30 HSP70 gene contigs in stony coral Pocillopora damicornis...
February 12, 2018: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29435658/correction-to-the-capos-mutation-in-atp1a3-alters-na-k-atpase-function-and-results-in-auditory-neuropathy-which-has-implications-for-management
#20
Lisbeth Tranebjærg, Nicola Strenzke, Sture Lindholm, Nanna D Rendtorff, Hanne Poulsen, Himanshu Khandelia, Wojciech Kopec, Troels J Brünnich Lyngbye, Christian Hamel, Cecile Delettre, Beatrice Bocquet, Michael Bille, Hanne H Owen, Toke Bek, Hanne Jensen, Karen Østergaard, Claes Möller, Linda Luxon, Lucinda Carr, Louise Wilson, Kaukab Rajput, Tony Sirimanna, Katherine Harrop-Griffiths, Shamima Rahman, Barbara Vona, Julia Doll, Thomas Haaf, Oliver Bartsch, Hendrik Rosewich, Tobias Moser, Maria Bitner-Glindzicz
The following information was inadvertently omitted in the original publication.
February 12, 2018: Human Genetics
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