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https://www.readbyqxmd.com/read/29240795/the-msmx-atpase-plays-a-crucial-role-in-pectin-mobilization-by-bacillus-subtilis
#1
Mário J Ferreira, Aristides L Mendes, Isabel de Sá-Nogueira
Carbohydrates from plant cell walls are often found as heteropolysaccharides intertwined with each other. For competitive advantage against other microorganisms, and ability to fully exploit available carbon and energy sources, Bacillus subtilis possesses a high number of proteins dedicated to the uptake of mono- and oligosaccharides. Here, we characterize transporter complexes, belonging to the ATP-binding cassette (ABC) superfamily, involved in the uptake of oligosaccharides commonly found in pectin. The uptake of these carbohydrates is shown to be MsmX-dependent, assigning a key role in pectin mobilization for MsmX, a multipurpose ATPase serving several distinct ABC-type I sugar importers...
2017: PloS One
https://www.readbyqxmd.com/read/29239839/the-formin-diaph1-is-a-key-modulator-of-myocardial-ischemia-reperfusion-injury
#2
Karen M O'Shea, Radha Ananthakrishnan, Qing Li, Nosirudeen Quadri, Devi Thiagarajan, Gopalkrishna Sreejit, Lingjie Wang, Hylde Zirpoli, Juan Francisco Aranda, Arthur S Alberts, Ann Marie Schmidt, Ravichandran Ramasamy
The biochemical, ionic, and signaling changes that occur within cardiomyocytes subjected to ischemia are exacerbated by reperfusion; however, the precise mechanisms mediating myocardial ischemia/reperfusion (I/R) injury have not been fully elucidated. The receptor for advanced glycation end-products (RAGE) regulates the cellular response to cardiac tissue damage in I/R, an effect potentially mediated by the binding of the RAGE cytoplasmic domain to the diaphanous-related formin, DIAPH1. The aim of this study was to investigate the role of DIAPH1 in the physiological response to experimental myocardial I/R in mice...
November 21, 2017: EBioMedicine
https://www.readbyqxmd.com/read/29238994/intestinal-p-glycoprotein-inhibitors-benzoxanthone-analogues
#3
Song Wha Chae, Jaeok Lee, Jung Hyun Park, Youngjoo Kwon, Younghwa Na, Hwa Jeong Lee
OBJECTIVES: The inhibitors of P-glycoprotein (P-gp) which limits an access of exogenous compounds in the luminal membrane of the intestine have been studied to enhance the intestinal P-gp-mediated absorption of anticancer drugs. METHODS: Inhibition of the efflux pump by synthesized benzoxanthone derivatives was investigated in vitro and in vivo. MCF-7/ADR cell line was used for cytotoxicity assay and [3 H]-daunomycin (DNM) accumulation/efflux study. Eight benzoxanthone analogues were tested for their effects on DNM cytotoxicity...
December 13, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/29238611/structural-basis-for-nucleotide-modulated-p97-association-with-the-er-membrane
#4
Wai Kwan Tang, Ting Zhang, Yihong Ye, Di Xia
Association of the cytosolic AAA (ATPases associated with various cellular activities) protein p97 to membranes is essential for various cellular processes including endoplasmic reticulum (ER)-associated degradation. The p97 consists of two ATPase domains and an N domain that interacts with numerous cofactors. The N domain of p97 is known to undergo a large nucleotide-dependent conformation switch, but its physiological relevance is unclear. Here we show p97 is recruited to canine ER membranes predominantly by interacting with VCP-interacting membrane protein (VIMP), an ER-resident protein...
2017: Cell Discovery
https://www.readbyqxmd.com/read/29238559/evolution-to-environmental-contamination-ablates-the-circadian-clock-of-an-aquatic-sentinel-species
#5
Kayla D Coldsnow, Rick A Relyea, Jennifer M Hurley
Environmental contamination is a common cause of rapid evolution. Recent work has shown that Daphnia pulex, an important freshwater species, can rapidly evolve increased tolerance to a common contaminant, sodium chloride (NaCl) road salt. While such rapid evolution can benefit organisms, allowing them to adapt to new environmental conditions, it can also be associated with unforeseen tradeoffs. Given that exposure to environmental contaminants can cause circadian disruption, we investigated whether the circadian clock was affected by evolving a tolerance to high levels of road salt...
December 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/29238219/hypoxia-stress-modifies-na-k-atpase-h-k-atpase-formula-see-text-and-nka%C3%AE-1-isoform-expression-in-the-brain-of-immune-challenged-air-breathing-fish
#6
Mc Subhash Peter, Satheesan Simi
Fishes are equipped to sense stressful stimuli and are able to respond to environmental stressor such as hypoxia with varying pattern of stress response. The functional attributes of brain to hypoxia stress in relation to ion transport and its interaction during immune challenge have not yet delineated in fish. We, therefore, explored the pattern of ion transporter functions and messenger RNA (mRNA) expression of α1-subunit isoforms of Na+/K+-ATPase (NKA) in the brain segments, namely, prosencephalon (PC), mesencephalon (MC), and metencephalon (MeC) in an obligate air-breathing fish exposed either to hypoxia stress (30 minutes forced immersion in water) or challenged with zymosan treatment (25-200 ng g-1 for 24 hours) or both...
2017: Journal of Experimental Neuroscience
https://www.readbyqxmd.com/read/29237760/thorase-variants-are-associated-with-defects-in-glutamatergic-neurotransmission-that-can-be-rescued-by-perampanel
#7
George K E Umanah, Marco Pignatelli, Xiling Yin, Rong Chen, Joshua Crawford, Stewart Neifert, Leslie Scarffe, Adam A Behensky, Noah Guiberson, Melissa Chang, Erica Ma, Jin Wan Kim, Cibele C Castro, Xiaobo Mao, Li Chen, Shaida A Andrabi, Mikhail V Pletnikov, Ann E Pulver, Dimitrios Avramopoulos, Antonello Bonci, David Valle, Ted M Dawson, Valina L Dawson
The AAA+ adenosine triphosphatase (ATPase) Thorase plays a critical role in controlling synaptic plasticity by regulating the expression of surface α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs). Bidirectional sequencing of exons of ATAD1, the gene encoding Thorase, in a cohort of patients with schizophrenia and healthy controls revealed rare Thorase variants. These variants caused defects in glutamatergic signaling by impairing AMPAR internalization and recycling in mouse primary cortical neurons...
December 13, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/29237574/late-rise-in-hemolymph-osmolality-in-macrobrachium-acanthurus-diadromous-freshwater-shrimp-exposed-to-brackish-water-early-reduction-in-branchial-na-k-pump-activity-but-stable-muscle-hsp70-expression
#8
Carolina A Freire, Anieli C Maraschi, Alessandra F Lara, Enelise M Amado, Viviane Prodocimo
Some Macrobrachium shrimps (Caridea, Palaemonidae) are diadromous; freshwater adults are truly euryhaline, while larvae need saline water for development. Branchial Na+/K+-ATPase (NKA) and carbonic anhydrase (CA) are involved in NaCl absorption in freshwater. This study aimed at verifying the time course of the osmoregulatory response of adult Macrobrachium acanthurus to high salinity brackish water (20‰), from the first 30min to 5days. The goal was to detect possible transition from hyper- to hyporegulation, the putative involvement of branchial NKA and CA, or the induction of muscular HSP70 expression...
December 10, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29237407/identification-and-in-silico-characterization-of-a-novel-p-p208pfsx1-mutation-in-v-atpase-a3-subunit-associated-with-autosomal-recessive-osteopetrosis-in-a-pakistani-family
#9
Muhammad Ajmal, Asif Mir, Sughra Wahid, Chiea Chuen Khor, Jia Nee Foo, Saima Siddiqi, Mehran Kauser, Salman Akbar Malik, Muhammad Nasir
BACKGROUND: Osteopetrosis is a rare inherited bone disorder mainly described as an increased bone density caused by defective osteoclastic bone resorption. To date, genetic variants of eleven genes have been reported so far to be associated with different types of osteopetrosis. However, malignant infantile osteopetrosis, a lethal form of the disease, is mostly (50%) caused by mutation(s) in TCIRG1 gene. In this study, we investigated a consanguineous Pakistani family clinically and genetically to elucidate underlying molecular basis of the infantile osteopetrosis...
December 13, 2017: BMC Medical Genetics
https://www.readbyqxmd.com/read/29237113/the-replication-protein-cdc6-suppresses-centrosome-over-duplication-in-a-manner-independent-of-its-atpase-activity
#10
Gwang Su Kim, Inyoung Lee, Ji Hun Kim, Deog Su Hwang
The Cdc6 protein is essential for the initiation of chromosomal replication and functions as a licensing factor to maintain chromosome integrity. During the S and G2 phases of the cell cycle, Cdc6 has been found to inhibit the recruitment of pericentriolar material (PCM) proteins to the centrosome and to suppress centrosome over-duplication. In this report, we analyzed the correlation between these two functions of Cdc6 at the centrosome. Cdc6 depletion increased the population of cells showing centrosome over-duplication and premature centrosome separation; Cdc6 expression reversed these changes...
December 12, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29237037/insights-into-the-evolutionary-conserved-regulation-of-rio-atpase-activity
#11
Robert Knüppel, Regitse H Christensen, Fiona C Gray, Dominik Esser, Daniela Strauß, Jan Medenbach, Bettina Siebers, Stuart A MacNeill, Nicole LaRonde, Sébastien Ferreira-Cerca
Eukaryotic ribosome biogenesis is a complex dynamic process which requires the action of numerous ribosome assembly factors. Among them, the eukaryotic Rio protein family members (Rio1, Rio2 and Rio3) belong to an ancient conserved atypical protein kinase/ ATPase family required for the maturation of the small ribosomal subunit (SSU). Recent structure-function analyses suggested an ATPase-dependent role of the Rio proteins to regulate their dynamic association with the nascent pre-SSU. However, the evolutionary origin of this feature and the detailed molecular mechanism that allows controlled activation of the catalytic activity remained to be determined...
December 9, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29236392/effect-of-preparations-methyure-and-ivine-on-%C3%B0-%C3%B0-2-atpases-activity-in-plasma-and-vacuolar-membrane-of-corn-seedling-roots-under-salt-stress-conditions
#12
M V Rudnytska, T A Palladina
Ca2+-ATPases regulate the functioning of Ca2+-dependent signaling pathway SOS which provides removal of Na+ from the cytoplasm of cells via Na+/H+-antiporters in saline conditions. The influence of synthetic preparations Methyure and Ivine on the Ca2+-ATPase activity was investigated. It was shown that exposition of corn seedlings in the presence of 0.1 M NaCl rather enhanced hydrolytic than transport activity of Ca2+-ATPases in plasma and vacuolar membrane of root cells. It was found that seed treatment with such preparations, especially Methyure, caused intensification of the both activities of Ca2+-ATPases, mainly in vacuolar membrane...
January 2017: Ukrainian Biochemical Journal
https://www.readbyqxmd.com/read/29236154/calcium-dynamics-in-tomato-pollen-tubes-using-the-yellow-cameleon-3-6-sensor
#13
María Laura Barberini, Lorena Sigaut, Weijie Huang, Silvina Mangano, Silvina Paola Denita Juarez, Eliana Marzol, José Estevez, Mariana Obertello, Lía Pietrasanta, Weihua Tang, Jorge Muschietti
In vitro tomato pollen tubes show a cytoplasmic calcium gradient that oscillates with the same period as growth. Pollen tube growth requires coordination between the tip-focused cytoplasmic calcium concentration ([Ca2+]cyt) gradient and the actin cytoskeleton. This [Ca2+]cyt gradient is necessary for exocytosis of small vesicles, which contributes to the delivery of new membrane and cell wall at the pollen tube tip. The mechanisms that generate and maintain this [Ca2+]cyt gradient are not completely understood...
December 13, 2017: Plant Reproduction
https://www.readbyqxmd.com/read/29235532/investigation-of-the-effects-of-the-cftr-potentiator-ivacaftor-on-human-p-glycoprotein-abcb1
#14
Swathi Lingam, Nopnithi Thonghin, Robert C Ford
Ivacaftor is a potentiator of the CFTR chloride channel and is in worldwide clinical use for the chronic treatment of cystic fibrosis in patients. There is evidence that the bioavailability of ivacaftor in the body may be influenced by the multi-drug exporter P-glycoprotein. Here we have employed purified and reconstituted P-glycoprotein to study its interaction with ivacaftor as well as the ability of the drug to compete with a known transported substrate of the protein. We find that ivacaftor stimulates the ATPase activity of the purified protein and can compete with the transport of the fluorescent substrate Hoechst 33342...
December 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29235081/overview-of-the-mechanism-of-cytoskeletal-motors-based-on-structure
#15
REVIEW
Yusuke Kato, Takuya Miyakawa, Masaru Tanokura
In the last two decades, a wealth of structural and functional knowledge has been obtained for the three major cytoskeletal motor proteins, myosin, kinesin and dynein, which we review here. The cytoskeletal motor proteins myosin and kinesin are structurally similar in the core architecture of their motor domains and have similar force-producing mechanisms that are coupled with the chemical cycles of ATP binding, hydrolysis, Pi release and subsequent ADP release. The force is generated through conformational changes in the motor domain during Pi release and ATP binding in myosin and kinesin, respectively, and then converted into the rotation of the lever arm or neck linker (referred to as a power stroke) through the common structural pathways...
December 12, 2017: Biophysical Reviews
https://www.readbyqxmd.com/read/29234368/mechanisms-of-p-glycoprotein-modulation-by-semen-strychni-combined-with-radix-paeoniae-alba
#16
Li-Li Liu, Yong-Mei Guan, Xue-Ping Lu, Xin-Li Liang, Li-Hua Chen
Semen Strychni has been extensively used as a Chinese herb, but its therapeutic window is narrowed by the strong toxicity of the compound, which limits its effectiveness. Radix Paeoniae Alba has been reported to reduce the toxic effects and increase the therapeutic effects of Semen Strychni, but the underlying mechanism remains unknown. This research aimed to explore the mechanism through which P-glycoprotein (P-gp) is modulated by Semen Strychni combined with Radix Paeoniae Alba in vitro. An MTT assay was used to study cytotoxicity in an MDCK-MDR1 cell model...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29233943/atg-dependent-phagocytosis-in-dendritic-cells-drives-myelin-specific-cd4-t-cell-pathogenicity-during-cns-inflammation
#17
Christian W Keller, Christina Sina, Monika B Kotur, Giulia Ramelli, Sarah Mundt, Isaak Quast, Laure-Anne Ligeon, Patrick Weber, Burkhard Becher, Christian Münz, Jan D Lünemann
Although reactivation and accumulation of autoreactive CD4+ T cells within the CNS are considered to play a key role in the pathogenesis of multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE), the mechanisms of how these cells recognize their target organ and induce sustained inflammation are incompletely understood. Here, we report that mice with conditional deletion of the essential autophagy protein ATG5 in classical dendritic cells (DCs), which are present at low frequencies in the nondiseased CNS, are completely resistant to EAE development following adoptive transfer of myelin-specific T cells and show substantially reduced in situ CD4+ T cell accumulation during the effector phase of the disease...
December 12, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29233226/what-can-be-learned-about-the-enzyme-atpase-from-single-molecule-studies-of-its-subunit-f1
#18
Sándor Volkán-Kacso, Rudolph A Marcus
We summarize the different types of single molecule experiments on the F1 component of FOF1-ATP Synthase and what has been learned from them. We also describe results from our recent studies on interpreting the experiments using a chemical-mechanical theory for these biological motors.
January 2017: Quarterly Reviews of Biophysics
https://www.readbyqxmd.com/read/29231785/the-role-of-endomembrane-localized-vha-c-in-plant-growth
#19
Aimin Zhou, Tetsuo Takano, Shenkui Liu
In plant cells, the vacuolar-type H+-ATPase (V-ATPase), a large multisubunit endomembrane proton pump, plays an important role in acidification of subcellular organelles, pH and ion homeostasis, and endocytic and secretory trafficking. V-ATPase subunit c (VHA-c) is essential for V-ATPase assembly, and is directly responsible for binding and transmembrane transport of protons. In previous studies, we identified a PutVHA-c gene from Puccinellia tenuiflora, and investigated its function in plant growth. Subcellular localization revealed that PutVHA-c is mainly localized in endosomal compartments...
December 12, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/29230231/activation-of-the-lrr-receptor-like-kinase-psy1r-requires-transphosphorylation-of-residues-in-the-activation-loop
#20
Christian B Oehlenschlæger, Lotte B A Gersby, Nagib Ahsan, Jesper T Pedersen, Astrid Kristensen, Tsvetelina V Solakova, Jay J Thelen, Anja T Fuglsang
PSY1R is a leucine-rich repeat (LRR) receptor-like kinase (RLK) previously shown to act as receptor for the plant peptide hormone PSY1 (peptide containing sulfated tyrosine 1) and to regulate cell expansion. PSY1R phosphorylates and thereby regulates the activity of plasma membrane-localized H+-ATPases. While this mechanism has been studied in detail, little is known about how PSY1R itself is activated. Here we studied the activation mechanism of PSY1R. We show that full-length PSY1R interacts with members of the SERK co-receptor family in planta...
2017: Frontiers in Plant Science
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