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https://www.readbyqxmd.com/read/27914320/biochemical-precursor-effects-on-the-fatty-acid-production-in-cell-suspension-cultures-of-theobroma-cacao-l
#1
O Parra, A M Gallego, A Urrea, L F Rojas, C Correa, L Atehortúa
Cocoa butter (CB) is composed of 96% palmitic, stearic, oleic, linoleic and linolenic fatty acids that are responsible for the hardness, texture and fusion properties of chocolate. Through in vitro plant cell culture it is possible to modify CB lipid profiles and to study the fatty acid biosynthesis pathway on a subcellular level, evaluating fundamental aspects to enhance in vitro fatty acid production in a specific and controlled way. In this research, culture media was supplemented with acetate, biotin, pyruvate, bicarbonate and glycerol at three different concentrations and the effects on the biomass production (g/L), cell viability, and fatty acids profile and production was evaluated in in vitro cell suspensions culture...
November 25, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27913821/theobromine-upregulates-osteogenesis-by-human-mesenchymal-stem-cells-in-vitro-and-accelerates-bone-development-in-rats
#2
Bret H Clough, Joni Ylostalo, Elizabeth Browder, Eoin P McNeill, Thomas J Bartosh, H Ralph Rawls, Tetsuo Nakamoto, Carl A Gregory
Theobromine (THB) is one of the major xanthine-like alkaloids found in cacao plant and a variety of other foodstuffs such as tea leaves, guarana and cola nuts. Historically, THB and its derivatives have been utilized to treat cardiac and circulatory disorders, drug-induced nephrotoxicity, proteinuria and as an immune-modulator. Our previous work demonstrated that THB has the capacity to improve the formation of hydroxyl-apatite during tooth development, suggesting that it may also enhance skeletal development...
December 2, 2016: Calcified Tissue International
https://www.readbyqxmd.com/read/27871234/a-genome-wide-bac-end-sequence-survey-provides-first-insights-into-sweetpotato-ipomoea-batatas-l-lam-genome-composition
#3
Zengzhi Si, Bing Du, Jinxi Huo, Shaozhen He, Qingchang Liu, Hong Zhai
BACKGROUND: Sweetpotato, Ipomoea batatas (L.) Lam., is an important food crop widely grown in the world. However, little is known about the genome of this species because it is a highly heterozygous hexaploid. Gaining a more in-depth knowledge of sweetpotato genome is therefore necessary and imperative. In this study, the first bacterial artificial chromosome (BAC) library of sweetpotato was constructed. Clones from the BAC library were end-sequenced and analyzed to provide genome-wide information about this species...
November 21, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27833051/theobromine-up-regulates-cerebral-brain-derived-neurotrophic-factor-and-facilitates-motor-learning-in-mice
#4
Mitsugu Yoneda, Naotoshi Sugimoto, Masanori Katakura, Kentaro Matsuzaki, Hayate Tanigami, Akihiro Yachie, Takako Ohno-Shosaku, Osamu Shido
Theobromine, which is a caffeine derivative, is the primary methylxanthine produced by Theobroma cacao. Theobromine works as a phosphodiesterase (PDE) inhibitor to increase intracellular cyclic adenosine monophosphate (cAMP). cAMP activates the cAMP-response element-binding protein (CREB), which is involved in a large variety of brain processes, including the induction of the brain-derived neurotrophic factor (BDNF). BDNF supports cell survival and neuronal functions, including learning and memory. Thus, cAMP/CREB/BDNF pathways play an important role in learning and memory...
January 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/27818491/corrigendum-cacao-polyphenols-ameliorate-autoimmune-myocarditis-in-mice
#5
Hirofumi Zempo, Jun-Ichi Suzuki, Ryo Watanabe, Kouji Wakayama, Hidetoshi Kumagai, Yuichi Ikeda, Hiroshi Akazawa, Issei Komuro, Mitsuaki Isobe
No abstract text is available yet for this article.
November 2016: Hypertension Research: Official Journal of the Japanese Society of Hypertension
https://www.readbyqxmd.com/read/27790069/endophytic-association-of-trichoderma-asperellum-within-theobroma-cacao-suppresses-vascular-streak-dieback-incidence-and-promotes-side-graft-growth
#6
Ade Rosmana, Nasaruddin Nasaruddin, Hendarto Hendarto, Andi Akbar Hakkar, Nursalim Agriansyah
Trichoderma species are able to persist on living sapwood and leaves of cacao (Theobroma cacao) in an endophytic relationship. In this research, we evaluated the ability of Trichodema asperellum introduced at the incision site in the bark for side grafting with the concentration of 4 g/10 mL, 4 g/100 mL, and 4 g/1,000 mL (suspended in water) in suppressing vascular streak dieback (VSD) incidence and promoting growth of side grafts in the field. The incidence of VSD in two local clones of cacao, MCC1 and M04, without application of T...
September 2016: Mycobiology
https://www.readbyqxmd.com/read/27769412/prediction-of-fermentation-index-of-cocoa-beans-theobroma-cacao-l-based-on-color-measurement-and-artificial-neural-networks
#7
Noemí León-Roque, Mohamed Abderrahim, Luis Nuñez-Alejos, Silvia M Arribas, Luis Condezo-Hoyos
Several procedures are currently used to assess fermentation index (FI) of cocoa beans (Theobroma cacao L.) for quality control. However, all of them present several drawbacks. The aim of the present work was to develop and validate a simple image based quantitative procedure, using color measurement and artificial neural network (ANNs). ANN models based on color measurements were tested to predict fermentation index (FI) of fermented cocoa beans. The RGB values were measured from surface and center region of fermented beans in images obtained by camera and desktop scanner...
December 1, 2016: Talanta
https://www.readbyqxmd.com/read/27700988/preparative-separation-of-cacao-bean-procyanidins-by-high-speed-counter-current-chromatography
#8
Lingxi Li, Shuting Zhang, Yan Cui, Yuanyuan Li, Lanxin Luo, Peiyu Zhou, Baoshan Sun
In this work, an efficient method for preparative separation of procyanidins from raw cacao bean extract by high-speed counter-current chromatography (HSCCC) was developed. Under the optimized solvent system of n-hexane-ethyl acetate-water (1:50:50, v/v/v) with a combination of head-tail and tail-head elution modes, various procyanidins fractions with different polymerization degrees were successfully separated. UPLC, QTOF-MS and (1)H NMR analysis verified that these fractions contained monomer up to pentamer respectively...
September 27, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27625772/evaluation-of-different-fermentation-processes-for-use-by-small-cocoa-growers-in-mexico
#9
C Hernández-Hernández, Procopio Alejandro López-Andrade, Miguel A Ramírez-Guillermo, Diana Guerra Ramírez, Juan F Caballero Pérez
The aim of this research was to evaluate four different cacao (Theobroma cacao L.) fermentation conditions and their effect on fermented bean quality, in order to be able to recommend the most suitable condition to producers in the municipality of Huimanguillo, Tabasco, Mexico. Fermentations were carried out in square wooden boxes with capacity for 1000, 300, and 100 kg of fresh beans, as well as a rotary drum with capacity for 500 kg thereof. The fermentation process was carried out for 7 days, and the response variables measured were mass temperature, total soluble solids (TSS), pH, and acidity...
September 2016: Food Science & Nutrition
https://www.readbyqxmd.com/read/27617028/shade-tree-diversity-and-aboveground-carbon-stocks-in-theobroma-cacao-agroforestry-systems-implications-for-redd-%C3%A2-implementation-in-a-west-african-cacao-landscape
#10
Evans Dawoe, Winston Asante, Emmanuel Acheampong, Paul Bosu
BACKGROUND: The promotion of cacao agroforestry is one of the ways of diversifying farmer income and creating incentives through their inclusion in REDD+ interventions. We estimated the aboveground carbon stocks in cacao and shade trees, determined the floristic diversity of shade trees and explored the possibility of implementing REDD+ interventions in cacao landscapes. Using replicated multi-site transect approach, data were collected from nine 1-ha plots established on 5 km long transects in ten cacao growing districts in Ghana West Africa...
December 2016: Carbon Balance and Management
https://www.readbyqxmd.com/read/27604351/erratum-to-theobroma-cacao-l-pathogenesis-related-gene-tandem-array-members-show-diverse-expression-dynamics-in-response-to-pathogen-colonization
#11
Andrew S Fister, Luis C Mejia, Yufan Zhang, Edward Allen Herre, Siela N Maximova, Mark J Guiltinan
No abstract text is available yet for this article.
2016: BMC Genomics
https://www.readbyqxmd.com/read/27596387/theobroma-cacao-extract-attenuates-the-development-of-dermatophagoides-farinae-induced-atopic-dermatitis-like-symptoms-in-nc-nga-mice
#12
Heerim Kang, Chang Hyung Lee, Jong Rhan Kim, Jung Yeon Kwon, Myoung-Jin Son, Jong-Eun Kim, Ki Won Lee
Cacao beans from Theobroma cacao are an abundant source of polyphenols, particularly flavonoids. Previous studies demonstrated that cacao flavanols decrease pro-inflammatory cytokines resulting in the alleviation of allergic symptoms. We sought to investigate the effects of cacao extract (CE) on Dermatophagoides farinae extract (DFE)-induced atopic dermatitis (AD)-like symptoms. CE attenuated DFE-induced AD-like symptoms as assessed by skin lesion analyses, dermatitis score, and skin thickness. Histopathological analysis revealed that CE suppressed DFE-induced immune cell infiltration into the skin...
February 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27554321/cocoa-theobroma-cacao-polyphenol-rich-extract-increases-the-chronological-lifespan-of-saccharomyces-cerevisiae
#13
I Baiges, L Arola
BACKGROUND: Saccharomyces cerevisiae is a model organism with conserved aging pathways. Yeast chronological lifespan experiments mimic the processes involved in human non-dividing tissues, such as the nervous system or skeletal muscle, and can speed up the search for biomolecules with potential anti-aging effects before proceeding to animal studies. OBJECTIVE: To test the effectiveness of a cocoa polyphenol-rich extract (CPE) in expanding the S. cerevisiae chronological lifespan in two conditions: in the stationary phase reached after glucose depletion and under severe caloric restriction...
2016: Journal of Frailty & Aging
https://www.readbyqxmd.com/read/27534964/association-mapping-of-seed-and-disease-resistance-traits-in-theobroma-cacao-l
#14
Lambert A Motilal, Dapeng Zhang, Sue Mischke, Lyndel W Meinhardt, Michel Boccara, Olivier Fouet, Claire Lanaud, Pathmanathan Umaharan
Microsatellite and single nucleotide polymorphism markers that could be used in marker assisted breeding of cacao were identified for number of filled seeds, black pod resistance and witches' broom disease resistance. An association mapping approach was employed to identify markers for seed number and resistance to black pod and witches' broom disease (WBD) in cacao (Theobroma cacao L.). Ninety-five microsatellites (SSRs) and 775 single nucleotide polymorphisms (SNPs) were assessed on 483 unique trees in the International Cocoa Genebank Trinidad (ICGT)...
December 2016: Planta
https://www.readbyqxmd.com/read/27514442/modified-pectin-from-theobroma-cacao-induces-potent-pro-inflammatory-activity-in-murine-peritoneal-macrophage
#15
Juliana C Amorim, Lucia Cristina Vriesmann, Carmen L O Petkowicz, Glaucia Regina Martinez, Guilhermina R Noleto
In vitro effects of acetylated pectin (OP) isolated from cacao pod husks (Theobroma cacao L.), its partially deacetylated and de-esterified form (MOP), and a commercial homogalacturonan (PG) were investigated on murine peritoneal macrophages. MOP stood out among the studied pectins. After 48h of incubation, compared with the control group, it was able to promote significant macrophage morphological differentiation from resident to activated stage and also stimulated nitric oxide production, which reached a level of 85% of that of LPS stimulus...
November 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27504627/diallel-analysis-and-growth-parameters-as-selection-tools-for-drought-tolerance-in-young-theobroma-cacao-plants
#16
Emerson Alves Dos Santos, Alex-Alan Furtado de Almeida, Dario Ahnert, Marcia Christina da Silva Branco, Raúl René Valle, Virupax C Baligar
This study aimed to estimate the combining ability, of T. cacao genotypes preselected for drought tolerance through diallel crosses. The experiment was conducted under greenhouse conditions at the Cacao Research Center (CEPEC), Ilhéus, Bahia, Brazil, in a completely randomized block design, in an experimental arrangement 21 x 2 [21 complete diallel crosses and two water regimes (control and stressed)]. In the control, soil moisture was kept close to field capacity, with predawn leaf water potential (ΨWL) ranging from -0...
2016: PloS One
https://www.readbyqxmd.com/read/27501038/-chocolate-silver-nanoparticles-synthesis-antibacterial-activity-and-cytotoxicity
#17
Neelika Roy Chowdhury, Melanie MacGregor-Ramiasa, Peter Zilm, Peter Majewski, Krasimir Vasilev
HYPOTHESIS: Silver nanoparticles (AgNPs) have emerged as a powerful weapon against antibiotic resistant microorganisms. However, most conventional AgNPs syntheses require the use of hazardous chemicals and generate toxic organic waste. Hence, in recent year's, plant derived and biomolecule based synthetics have has gained much attention. Cacao has been used for years for its medicinal benefits and contains a powerful reducing agent - oxalic acid. We hypothesized that, due to the presence of oxalic acid, cacao extract is capable of reducing silver nitrate (AgNO3) to produce AgNPs...
November 15, 2016: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/27491435/fungal-and-oomycete-diseases-of-tropical-tree-fruit-crops
#18
André Drenth, David I Guest
The tropics produce a range of fruit from tree crops that cannot be grown in colder climates. Bananas, mangos, several nuts, spices, coffee, and cacao are widely traded and much sought after around the world. However, the sustainable production of these tropical tree fruit crops faces significant challenges. Among these, losses due to pests and diseases play a large part in reducing yields, quality, and profitability. Using bananas and cacao as key examples, we outline some of the reasons fungal and oomycete diseases cause such significant losses to tropical tree crops...
August 4, 2016: Annual Review of Phytopathology
https://www.readbyqxmd.com/read/27470560/procyanidin-rich-extract-of-natural-cocoa-powder-causes-ros-mediated-caspase-3-dependent-apoptosis-and-reduction-of-pro-mmp-2-in-epithelial-ovarian-carcinoma-cell-lines
#19
Shruti Sanjay Taparia, Aparna Khanna
Over the last four centuries, cocoa and chocolate have been described as having potential medicinal value. As of today, Theobroma cacao L. (Sterculiaceae) and its products are consumed worldwide. They are of great research interest because of the concentration dependent antioxidant as well as pro-oxidant properties of some of their polyphenolic constituents, specially procyanidins and flavan-3-ols such as catechin. This study was aimed at investigating the cellular and molecular changes associated with cytotoxicity, caused due pro-oxidant activity of cocoa catechins and procyanidins, in ovarian cancer cell lines...
June 24, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27454588/expression-of-the-theobroma-cacao-bax-inhibitor-1-gene-in-tomato-reduces-infection-by-the-hemibiotrophic-pathogen-moniliophthora-perniciosa
#20
Danielle Camargo Scotton, Mariana da Silva Azevedo, Ivan Sestari, Jamille Santos da Silva, Lucas Anjos Souza, Lázaro Eustáquio Pereira Peres, Gildemberg Amorim Leal, Antonio Figueira
Programmed cell death (PCD) plays a key role in plant responses to pathogens, determining the success of infection depending on the pathogen lifestyle and on which participant of the interaction triggers cell death. The hemibiotrophic basidiomycete Moniliophthora perniciosa is the causal agent of the witches' broom disease of Theobroma cacao L (cacao), a serious constraint for production in South America and Caribbean. It has been hypothesized that M. perniciosa pathogenesis involves PCD, initially as a plant defense mechanism, which is diverted by the fungus to induce necrosis during the dikaryotic phase of the mycelia...
July 25, 2016: Molecular Plant Pathology
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