Ke Fang, Ziting Xu, Lu Yang, Quan Cui, Bowen Du, Huijing Liu, Ruiming Wang, Piwu Li, Jing Su, Junqing Wang
10-Hydroxy-2-decenoic acid (10-HDA) is an important component of royal jelly, known for its antimicrobial, anti-inflammatory, blood pressure-lowering, and antiradiation effects. Currently, 10-HDA biosynthesis is limited by the substrate selectivity of acyl-coenzyme A dehydrogenase, which restricts the technique to a two-step process. This study aimed to develop an efficient and simplified method for synthesizing 10-HDA. In this study, ACOX from Candida tropicalis 1798, which catalyzes 10-hydroxydecanoyl coenzyme A desaturation for 10-HDA synthesis, was isolated and heterologously coexpressed with FadE, Macs, YdiI, and CYP in Escherichia coli /SK after knocking out FadB, FadJ, and FadR genes...
January 4, 2024: Journal of Agricultural and Food Chemistry