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https://www.readbyqxmd.com/read/28448172/the-macrophage-stimulating-anti-cancer-agent-rrx-001-protects-against-ischemia-reperfusion-injury
#1
Pedro Cabrales, Scott Caroen, Arnold Oronsky, Corey Carter, Jane Trepel, Thomas Summers, Tony Reid, Neil Oronsky, Michelle Lybeck, Bryan Oronsky
Introduction RRx-001, a clinical macrophage-stimulating anti-cancer agent that also produces nitric oxide (NO) was studied in a model of ischemia-reperfusion injury. Methods The production of NO is dependent on the oxygen tension because nitric oxide synthases convert l-arginine to NO and l-citrulline in the presence of O2. Since the P450 enzymes, which metabolize nitrate esters such as nitroglycerin are dependent on oxygen, the generation of 'exogenous' NO is also sensitive to alterations in tissue PO2. I/R injury was studied in a hamster chamber window, with compression of the periphery of the window for 1 hour to induce ischemia...
April 27, 2017: Expert Review of Hematology
https://www.readbyqxmd.com/read/28445766/inhibitory-effect-of-emulsifiers-in-sedds-on-protease-activity-just-an-illusion
#2
Noemi Lupo, Una Fidahic, Gergely Hetényi, Janine Griesser, Andreas Bernkop-Schnürch
AIM: Evaluation of inhibitory effect of emulsifiers on pancreatic trypsin and α-chymotrypsin. METHODS: The inhibitory effect of Cremophor EL, Cremophor RH 40, Brij O10, Tween 20, polyethylene glycol 8000, polyethylene glycol 400, Carbitol, Pemulen TR-1, Pemulen TR-2, Carbopol Ultrez 20 and Carbopol Ultrez 21 on pancreatic trypsin and α-chymotrypsin was tested. BAEE (Nα-Benzoyl-L-arginine ethyl ester), BTEE (N-Benzoyl-L-tyrosine ethyl ester), casein and insulin were used as substrates for trypsin and α-chymotrypsin...
April 23, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28444774/protective-effect-of-l-carnitine-and-l-arginine-against-busulfan-induced-oligospermia-in-adult-rat
#3
A M Abd-Elrazek, O A H Ahmed-Farid
Busulfan is an anticancer drug caused variety of adverse effects for patients with cancer. But it could cause damage to the male reproductive system as one of its adverse effects. This study aimed to investigate the protective effect of L-carnitine and L-arginine on semen quality, oxidative stress parameters and testes cell energy after busulfan treatment. Adult male rats were divided into four groups: control (Con), busulfan (Bus), busulfan plus L-arginine (Bus + L-arg) and busulfan plus L-carnitine (Bus + L-car)...
April 25, 2017: Andrologia
https://www.readbyqxmd.com/read/28443955/a-single-resistance-exercise-session-improves-aortic-endothelial-function-in-hypertensive-rats
#4
Thaís de Oliveira Faria, Jhuli Keli Angeli, Luiz Guilherme Marchesi Mello, Gustavo Costa Pinto, Ivanita Stefanon, Dalton Valentim Vassallo, Juliana Hott de Fúcio Lizardo
BACKGROUND: Physical exercise is an important tool for the improvement of endothelial function. OBJECTIVE: To assess the effects of acute dynamic resistance exercise on the endothelial function of spontaneously hypertensive rats (SHR). METHODS: Ten minutes after exercise, the aorta was removed to evaluate the expression of endothelial nitric oxide synthase (eNOS), phosphorylated endothelial nitric oxide synthase (p-eNOS1177) and inducible nitric oxide synthase (iNOS) and to generate concentration-response curves to acetylcholine (ACh) and to phenylephrine (PHE)...
March 2017: Arquivos Brasileiros de Cardiologia
https://www.readbyqxmd.com/read/28443021/functional-and-biochemical-endothelial-profiling-in-vivo-in-a-murine-model-of-endothelial-dysfunction-comparison-of-effects-of-1-methylnicotinamide-and-angiotensin-converting-enzyme-inhibitor
#5
Anna Bar, Mariola Olkowicz, Urszula Tyrankiewicz, Edyta Kus, Krzysztof Jasinski, Ryszard T Smolenski, Tomasz Skorka, Stefan Chlopicki
Although it is known that 1-methylnicotinamide (MNA) displays vasoprotective activity in mice, as yet the effect of MNA on endothelial function has not been demonstrated in vivo. Here, using magnetic resonance imaging (MRI) we profile the effects of MNA on endothelial phenotype in mice with atherosclerosis (ApoE/LDLR(-/-)) in vivo, in comparison to angiotensin (Ang) -converting enzyme (ACE) inhibitor (perindopril), with known vasoprotective activity. On a biochemical level, we analyzed whether MNA- or perindopril-induced improvement in endothelial function results in changes in ACE/Ang II-ACE2/Ang-(1-7) balance, and L-arginine/asymmetric dimethylarginine (ADMA) ratio...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28442706/nitric-oxide-no-mediates-the-inhibition-of-form-deprivation-myopia-by-atropine-in-chicks
#6
Brittany J Carr, William K Stell
Myopia is the most common childhood refractive disorder. Atropine inhibits myopia progression, but its mechanism is unknown. Here, we show that myopia-prevention by atropine requires production of nitric oxide (NO). Form-deprivation myopia (FDM) was induced in week-old chicks by diffusers over the right eye (OD); the left eye (OS) remained ungoggled. On post-goggling days 1, 3, and 5, OD received intravitreally 20 µL of phosphate-buffered saline (vehicle), or vehicle plus: NO source: L-arginine (L-Arg, 60-6,000 nmol) or sodium nitroprusside (SNP, 10-1,000 nmol); atropine (240 nmol); NO inhibitors: L-NIO or L-NMMA (6 nmol); negative controls: D-Arg (10 µmol) or D-NMMA (6 nmol); or atropine plus L-NIO, L-NMMA, or D-NMMA; OS received vehicle...
December 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/28440889/a-glucagon-like-peptide-1-analog-liraglutide-improves-visceral-sensation-and-gut-permeability-in-rats
#7
Tsukasa Nozu, Saori Miyagishi, Shima Kumei, Rintaro Nozu, Kaoru Takakusaki, Toshikatsu Okumura
BACKGROUND AND AIM: A glucagon-like peptide-1 (GLP-1) analog, liraglutide, has been reported to block inflammatory somatic pain. We hypothesized that liraglutide attenuates lipopolysaccharide (LPS)- and repeated water avoidance stress (WAS)-induced visceral hypersensitivity and tested the hypothesis in rats. METHODS: The threshold of the visceromotor response (VMR) induced by colonic balloon distention was measured to assess visceral sensation. Colonic permeability was determined in vivo by quantifying the absorbed Evans blue spectrophotometrically, which was instilled in the proximal colon for 15 min...
April 25, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28439717/negligible-effect-of-enos-palmitoylation-on-fatty-acid-regulation-of-contraction-in-ventricular-myocytes-from-healthy-and-hypertensive-rats
#8
Chun Li Jin, Yu Na Wu, Ji Hyun Jang, Zai Hao Zhao, Goo Taeg Oh, Sung Joon Kim, Yin Hua Zhang
S-palmitoylation is an important post-translational modification that affects the translocation and the activity of target proteins in a variety of cell types including cardiomyocytes. Since endothelial nitric oxide synthase (eNOS) is known to be palmitoylated and the activity of eNOS is essential in fatty acid-dependent β-oxidation in muscle, we aimed to test whether palmitoylation of eNOS is involved in palmitic acid (PA) regulation of left ventricular (LV) myocyte contraction from healthy (sham) and hypertensive (HTN) rats...
April 25, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28438687/dietary-nitrite-supplementation-attenuates-cardiac-remodeling-in-l-name-induced-hypertensive-rats
#9
Kunihiro Sonoda, Kazuo Ohtake, Hiroyuki Uchida, Junta Ito, Masaki Uchida, Hideshi Natsume, Hazuki Tamada, Jun Kobayashi
Loss of nitric oxide (NO) bioavailability underlies the development of hypertensive heart disease. We investigated the effects of dietary nitrite on N(G)-nitro-l-arginine methyl ester (l-NAME)-induced hypertension. Sprague-Dawley rats were divided into five groups: an untreated control group, an l-NAME-treated group, and three other l-NAME-treated groups supplemented with 10 mg/L or 100 mg/L of nitrite or 100 mg/L of captopril in drinking water. After the 8-week experimental period, mean arterial blood pressure was measured, followed by sampling of blood and heart tissue for assessment of nitrite/nitrate levels in the plasma and heart, the plasma level of angiotensin II (AT II), and the heart transcriptional levels of AT II type 1 receptor (AT1R), transforming growth factor-β1 (TGF-β1), and connective tissue proteins such as type 1 collagen and fibronectin...
April 21, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28436051/a-novel-mass-spectrometry-based-method-for-simultaneous-determination-of-asymmetric-and-symmetric-dimethylarginine-l-arginine-and-l-citrulline-optimized-for-lc-ms-tof-and-lc-ms-ms
#10
Jerzy Wiśniewski, Mariusz G Fleszar, Joanna Piechowicz, Małgorzata Krzystek-Korpacka, Angelika Chachaj, Andrzej Szuba, Katarzyna Lorenc-Kukula, Leszek Masłowski, Wojciech Witkiewicz, Andrzej Gamian
Nitric oxide (NO) is a regulatory molecule involved in many biological processes. NO is produced by nitric oxide synthase by conversion of L-arginine to L-citrulline. L-arginine methylated derivatives, asymmetric and symmetric dimethylarginines (ADMA and SDMA), regulate L-arginine availability and the activity of nitric oxide synthase. As such, they have been frequently investigated as potential biomarkers in pathologies associated with dysfunctions in NO synthesis. Here, we present a new multistep analytical methodology based on liquid chromatography combined with mass spectrometry for the accurate identification of L-arginine, L-citrulline, ADMA, and SDMA...
April 24, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28433656/impaired-endothelium-derived-hyperpolarization-type-relaxation-in-superior-mesenteric-arteries-isolated-from-female-otsuka-long-evans-tokushima-fatty-rats
#11
Takayuki Matsumoto, Shota Kobayashi, Makoto Ando, Shun Watanabe, Maika Iguchi, Kumiko Taguchi, Tsuneo Kobayashi
Endothelium-derived hyperpolarization (EDH) is an important signaling mechanism of endothelium-dependent vasorelaxation, and little attention has been paid to the EDH-type responses in female metabolic syndrome such as that observed with type-2 diabetes. We previously reported that EDH-type relaxation was impaired in superior mesenteric arteries from male Otsuka Long-Evans Tokushima Fatty (OLETF) rat, a model of type-2 diabetes, however, the response was unclear in female OLETF rat. Thus, the aim of this study was to examine if EDH-type relaxation was altered in superior mesenteric arteries isolated from female OLETF rats compared to age-matched, control female Long-Evans Tokushima Otsuka (LETO) rats at age 50-59 weeks...
April 19, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28431974/unique-mitochondrial-localization-of-arginase-1-and-2-in-hepatocytes-of-air-breathing-walking-catfish-clarias-batrachus-and-their-differential-expression-patterns-under-hyper-ammonia-stress
#12
Bodhisattwa Banerjee, Debaprasad Koner, Priyanka Lal, Nirmalendu Saha
Arginase (ARG) catalyzes the final step of ornithine-urea cycle (OUC) leading to a conversion of L-arginine to L-ornithine and urea. Several isoforms of ARG have been reported in vertebrates, out of which the two predominant isoforms are the cytosolic ARG1 and the mitochondrial ARG2. The air-breathing walking catfish (Clarias batrachus) is frequently being challenged by different environmental insults such as hyper-ammonia, dehydration and osmotic stresses in their natural habitats throughout the year. The present study investigated the active presence of ARG1 and ARG2 isoforms in hepatocytes along with unique localization of both the isoforms inside the mitochondria, and also their specific expression patterns under hyper-ammonia stress (5mM NH4Cl) in isolated hepatocytes of walking catfish...
April 18, 2017: Gene
https://www.readbyqxmd.com/read/28429459/nitric-oxide-donors-nitrates-l-arginine-or-nitric-oxide-synthase-inhibitors-for-acute-stroke
#13
REVIEW
Philip Mw Bath, Kailash Krishnan, Jason P Appleton
BACKGROUND: Nitric oxide (NO) has multiple effects that may be beneficial in acute stroke, including lowering blood pressure, and promoting reperfusion and cytoprotection. Some forms of nitric oxide synthase inhibition (NOS-I) may also be beneficial. However, high concentrations of NO are likely to be toxic to brain tissue. This is an update of a Cochrane review first published in 1998, and last updated in 2002. OBJECTIVES: To assess the safety and efficacy of NO donors, L-arginine, and NOS-I in people with acute stroke...
April 21, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28427959/beneficial-effects-of-heparin-and-l-arginine-on-dermonecrosis-effect-induced-by-vipera-lebetina-venom-involvement-of-no-in-skin-regeneration
#14
Nouara Bennacef-Heffar, Fatima Laraba-Djebari
It is well known that snake venoms such as Viperidae caused severe local effects such as hemorrhage, myonecrosis and dermonecrosis which can lead to permanent tissue loss or the disability. The aim of this study is to evaluate the skin regeneration using heparin and l-arginine as well as the dermonecrotic effects induced by Vipera lebetina venom (VLV). To better understand the toxic effects induced by VLV and to prevent or treat these effects, we evaluate the local effects and the skin regeneration with or without drugs...
April 17, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28423506/granulocyte-colony-stimulating-factor-enhances-the-therapeutic-efficacy-of-bone-marrow-mesenchymal-stem-cell-transplantation-in-rats-with-experimental-acute-pancreatitis
#15
Bo Qu, Yanjie Chu, Fang Zhu, Beibei Wang, Ting Liu, Bo Yu, Shizhu Jin
INTRODUCTION: Acute pancreatitis (AP) is one of the most common diseases involving necrotic inflammation. Bone marrow mesenchymal stem cells (BMMSCs) have the potential of multi-directional differentiation and self-renewal for tissue repair. It remains less clear if granulocyte colony-stimulating factor (G-CSF) can improve the therapeutic effect of BMMSC transplant in AP. Therefore, we explored this issue in a rat model of experimental AP. RESULTS: Transplanted PKH26-positive BMMSCs were present in the injured pancreatic tissue, with some cells co-expressed pancreatic cellular markers, including Pax-4, Ngn3 and Nkx-6...
March 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28423345/homocysteine-induced-peripheral-microcirculation-dysfunction-in-zebrafish-and-its-attenuation-by-l-arginine
#16
Sang Joon Lee, Sung Ho Park, Jinhyuk Fred Chung, Woorak Choi, Hyung Kyu Huh
Elevated blood homocysteine (Hcy) level is frequently observed in aged individuals and those with age-related vascular diseases. However, its effect on peripheral microcirculation is still not fully understood. Using in vivo zebrafish model, the degree of Hcy-induced peripheral microcirculation dysfunction is assessed in this study with a proposed dimensionless velocity parameter V¯CV/V¯PCV, where V¯CV and V¯PCV represent the peripheral microcirculation perfusion and the systemic perfusion levels, respectively...
April 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28420789/structural-and-stereoelectronic-insights-into-oxygenase-catalyzed-formation-of-ethylene-from-2-oxoglutarate
#17
Zhihong Zhang, Tristan J Smart, Hwanho Choi, Florence Hardy, Christopher T Lohans, Martine I Abboud, Melodie S W Richardson, Robert S Paton, Michael A McDonough, Christopher J Schofield
Ethylene is important in industry and biological signaling. In plants, ethylene is produced by oxidation of 1-aminocyclopropane-1-carboxylic acid, as catalyzed by 1-aminocyclopropane-1-carboxylic acid oxidase. Bacteria catalyze ethylene production, but via the four-electron oxidation of 2-oxoglutarate to give ethylene in an arginine-dependent reaction. Crystallographic and biochemical studies on the Pseudomonas syringae ethylene-forming enzyme reveal a branched mechanism. In one branch, an apparently typical 2-oxoglutarate oxygenase reaction to give succinate, carbon dioxide, and sometimes pyrroline-5-carboxylate occurs...
April 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28420139/interplay-between-oxidative-stress-and-nutrient-sensing-signaling-in-the-developmental-origins-of-cardiovascular-disease
#18
REVIEW
You-Lin Tain, Chien-Ning Hsu
Cardiovascular disease (CVD) presents a global health burden, despite recent advances in management. CVD can originate from early life by so-called "developmental origins of health and disease" (DOHaD). Epidemiological and experimental evidence supports that early-life insults can induce programming of later CVD. Underlying the DOHaD concept, early intervention may offset programming process to prevent the development of CVD, namely reprogramming. Oxidative stress and nutrient sensing signals have been considered to be major mechanisms of cardiovascular programming, while the interplay between these two mechanisms have not been examined in detail...
April 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28419516/systemic-and-local-anti-nociceptive-effects-of-simvastatin-in-the-rat-formalin-assay-role-of-peroxisome-proliferator-activated-receptor-%C3%AE-and-nitric-oxide
#19
Mohammad Taghi Mansouri, Bahareh Naghizadeh, Behnam Ghorbanzadeh, Soheila Alboghobeish
This study aimed to determine the potential systemic and local anti-nociceptive effects of simvastatin (SIM) and the possible role of peroxisome proliferator-activated receptor gamma (PPARγ) and nitric oxide (NO) pathways using a formalin assay in rats. After allocation, rats were intraplantarly (i.pl.) treated with formalin solution (2.5%) and the flinching behaviors were recorded for 5 min (phase 1) and 15-60 min (phase 2). SIM was given intraperitoneally (i.p.) and i.pl. 30 and 20 min before test, respectively...
April 17, 2017: Journal of Neuroscience Research
https://www.readbyqxmd.com/read/28415785/the-role-of-nitric-oxide-pathway-in-arginine-transport-and-growth-of-ipec-1-cells
#20
Hao Xiao, Liming Zeng, Fangyuan Shao, Bo Huang, Miaomiao Wu, Bie Tan, Yulong Yin
L-Arginine itself and its metabolite-nitric oxide play great roles in intestinal physiology. However, the molecular mechanism underlying nitric oxide pathway regulating L-Arginine transport and cell growth is not yet fully understood. We report that inhibition of nitric oxide synthase (NOS) significantly induced cell apoptosis (p < 0.05), and promoted the rate of Arginine uptake and the expressions of protein for CAT-2 and y+LAT-1 (p < 0.05), while reduced protein expression of CAT-1. And NOS inhibition markedly decreased the activation of mammalian target of rapamycin (mTOR) and PI3K-Akt pathways by Arginine in the IPEC-1 cells (p < 0...
March 16, 2017: Oncotarget
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