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https://www.readbyqxmd.com/read/29156278/enhanced-phenanthrene-degradation-in-river-sediments-using-a-combination-of-biochar-and-nitrate
#1
Xunan Yang, Zefang Chen, Qunhe Wu, Meiying Xu
Polycyclic aromatic hydrocarbons (PAHs) pollution in urban river sediments is a serious problem to ecological systems and human health. We examined novel remediation approaches, using a biochar amendment combined with bioaugmentation or/and nitrate stimulation, to degrade phenanthrene in sediment. Biochar amendment combined with nitrate stimulation enhanced phenanthrene degradation by 2.3 times that of the control and 1.9 times that of biochar alone. Nitrate stimulation altered the microbial succession and encouraged the growth of potential nitrate-reducing PAH-degraders Thiobacillus and Stenotrophomonas...
November 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29155201/biochemical-properties-of-the-htra-homolog-from-bacterium-stenotrophomonas-maltophilia
#2
Urszula Zarzecka, Anna Modrak-Wojcik, Martyna Bayassi, Maciej Szewczyk, Artur Gieldon, Adam Lesner, Tomasz Koper, Agnieszka Bzowska, Maurizio Sanguinetti, Steffen Backert, Barbara Lipinska, Joanna Skorko-Glonek
The HtrA proteins due to their proteolytic, and in many cases chaperone activity, efficiently counteract consequences of stressful conditions. In the environmental bacterium and nosocomial pathogen Stenotrophomonas maltophilia HtrA (HtrASm) is induced as a part of adaptive response to host temperature (37°C). We examined the biochemical properties of HtrASm and compared them with those of model HtrAEc from Escherichia coli. We found that HtrASm is a protease and chaperone that operates over a wide range of pH and is highly active at temperatures between 35-37°C...
November 16, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29152974/biocompatible-copper-oxide-nanoparticle-composites-from-cellulose-and-chitosan-facile-synthesis-unique-structure-and-antimicrobial-activity
#3
Chieu D Tran, James Makuvaza, Erik Munson, Brian Bennett
Copper in various forms has been known to have bactericidal activity. Challenges to its application include preventing mobilization of the copper, to both extend activity and avoid toxicity, and bioincompatibility of many candidate substrates for copper-immobilization. Using a simple ionic liquid, butylmethylimmidazolium chloride as the solvent, we developed a facile and green method to synthesize biocompatible composites containing copper oxide nanoparticles (CuONPs) from cellulose (CEL) and chitosan (CS) or CEL and keratin (KER)...
November 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29146601/airway-microbiota-across-age-and-disease-spectrum-in-cystic-fibrosis
#4
Edith T Zemanick, Brandie D Wagner, Charles E Robertson, Richard C Ahrens, James F Chmiel, John P Clancy, Ronald L Gibson, William T Harris, Geoffrey Kurland, Theresa A Laguna, Susanna A McColley, Karen McCoy, George Retsch-Bogart, Kurtis T Sobush, Pamela L Zeitlin, Mark J Stevens, Frank J Accurso, Scott D Sagel, J Kirk Harris
Our objectives were to characterise the microbiota in cystic fibrosis (CF) bronchoalveolar lavage fluid (BALF), and determine its relationship to inflammation and disease status.BALF from paediatric and adult CF patients and paediatric disease controls undergoing clinically indicated bronchoscopy was analysed for total bacterial load and for microbiota by 16S rDNA sequencing.We examined 191 BALF samples (146 CF and 45 disease controls) from 13 CF centres. In CF patients aged <2 years, nontraditional taxa (e...
November 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/29141235/bioremediation-of-pcb-contaminated-shallow-river-sediments-the-efficacy-of-biodegradation-using-individual-bacterial-strains-and-their-consortia
#5
Hana Horváthová, Katarína Lászlová, Katarína Dercová
Elimination of dangerous toxic and hydrophobic chlorinated aromatic compounds, mainly PCBs from the environment, is one of the most important aims of the environmental biotechnologies. In this work, biodegradation of an industrial mixture of PCBs (Delor 103, equivalent to Aroclor 1242) was performed using bacterial consortia composed of four bacterial strains isolated from the historically PCB-contaminated sediments and characterized as Achromobacter xylosoxidans, Stenotrophomonas maltophilia, Ochrobactrum anthropi and Rhodococcus ruber...
November 3, 2017: Chemosphere
https://www.readbyqxmd.com/read/29138886/clinical-predictors-of-stenotrophomonas-maltophilia-bacteremia-in-adult-patients-with-hematologic-malignancy
#6
Si-Ho Kim, Sun Young Cho, Cheol-In Kang, Hyeri Seok, Kyungmin Huh, Young Eun Ha, Doo Ryeon Chung, Nam Yong Lee, Kyong Ran Peck, Jae-Hoon Song
Stenotrophomonas maltophilia (SM) has emerged as an important nosocomial pathogen with high morbidity and mortality. Because of its unique antimicrobial susceptibility pattern, appropriate antimicrobial therapy for SM bacteremia is still challenging, especially in immunocompromised patients. The present study was performed to assess clinical predictors of SM bacteremia in adult patients with hematologic malignancy. From 2006 through 2016, a case-control study was performed at a tertiary-care hospital. Case patients were defined as SM bacteremia in patients with hematologic malignancy...
November 14, 2017: Annals of Hematology
https://www.readbyqxmd.com/read/29138446/antimicrobial-resistance-and-genetic-diversity-in-ceftazidime-non-susceptible-bacterial-pathogens-from-ready-to-eat-street-foods-in-three-taiwanese-cities
#7
Lin Lin, Sheng-Fan Wang, Tsung-Ying Yang, Wei-Chun Hung, Min-Yu Chan, Sung-Pin Tseng
Bacterial contamination of ready-to-eat (RTE) street foods is a major concern worldwide. Dissemination of antibiotic resistant pathogens from food is an emerging public-health threat. To investigate the prevalence of antibiotic resistance genes and ceftazidime resistance-associated efflux pumps in foodborne pathogens, 270 RTE street foods samples were collected in three densely populated Taiwanese cities. Among 70 ceftazidime non-susceptible isolates, 21 Stenotrophomonas maltophilia, 12 Pseudomonas spp., 22 Acinetobacter spp...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29133644/stenotrophomonas-maltophilia-an-emerging-entity-for-cluster-endophthalmitis
#8
Sarita Beri, Anurag Shandil, Rajiv Garg
PURPOSE: This was a study of acute cluster endophthalmitis along with clinical features, culture results, and visual outcomes of 10 eyes of 10 patients after intravitreal injection of Avastin (bevacizumab) in one sitting from a single vial. METHODS: Retrospective review of intravitreal injection of 1.25 mg/0.05 ml bevacizumab that was given to 10 eyes of 10 patients on the same day from a freshly opened vial. All patients manifested with endophthalmitis the next day...
November 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29126943/suge-belongs-to-the-small-multidrug-resistance-smr-protein-family-involved-in-tributyltin-tbt-biodegradation-and-bioremediation-by-alkaliphilic-stenotrophomonas-chelatiphaga-hs2
#9
Hamdy A Hassan
Tributyltin (TBT) used in a variety of industrial processes, subsequent discharge into the environment, its fate, toxicity and human exposure are topics of current concern. TBT degradation by alkaliphilic bacteria may be a key factor in the remediation of TBT in high pH contaminated sites. In this study, Stenotrophomonas chelatiphaga HS2 were isolated and identified from TBT contaminated site in Mediterranean Sea. S. chelatiphaga HS2 has vigor capability to transform TBT into dibutyltin and monobutyltin (DBT and MBT) at pH 9 and 7% NaCl (w/v)...
November 7, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29124413/assessment-of-bacterial-diversity-in-hyalomma-aegyptium-h-marginatum-and-h-excavatum-ticks-through-tag-encoded-pyrosequencing
#10
Adem Keskin, Ahmet Bursali, David E Snow, Scot E Dowd, Saban Tekin
Ticks are among the most significant human-biting ectoparasites and they play a major role in transmission of many pathogenic agents to humans. In the present study, three species of Hyalomma ticks, Hyalomma aegyptium, H. marginatum and H. excavatum, were examined for the presence of zoonotic bacteria, both male and female ticks alike. Examination of microbial diversity with tag-encoded pyrosequencing indicates that H. marginatum and H. excavatum were more diversity rich than H. aegyptium. Although numerous pathogenic and non-pathogenic bacterial genera were detected, including Acidovorax, Bacillus, Bacteroides, Bdellovibrio, Clostridium, Curvibacter, Escherichia, Flavobacterium, Limnohabitans, Paenibacillus, Ralstonia, Sarcina, Sediminibacterium, Segetibacter Stenotrophomonas and Variovorax, the predominant zoonotic bacteria represented in these ticks were genera Borrelia, Francisella, and Rickettsia...
November 9, 2017: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/29096346/intraabdominal-abscess-caused-by-stenotrophomonas-maltophilia-a-case-report
#11
Toyomitsu Sawai, Sumako Yoshioka, Nobuko Matsuo, Naofumi Suyama, Hiroshi Mukae
INTRODUCTION: Stenotrophomonas maltophilia usually causes nosocomial infections, but intraabdominal abscesses or organ/space surgical site infection (SSI) secondary to this organism has been rarely reported. Here, we reported a rare case of SSI that presented as intraabdominal abscess caused by S. maltophilia. PRESENTATION OF CASE: A 68-year-old woman presented to our hospital with transverse colon cancer. Further work up with abdominal computed tomography (CT) revealed left renal cell carcinoma...
October 27, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/29095153/periplasmic-form-of-dipeptidyl-aminopeptidase-iv-from-pseudoxanthomonas-mexicana-wo24-purification-kinetic-characterization-crystallization-and-x-ray-crystallographic-analysis
#12
Saori Roppongi, Chika Tateoka, Mayu Fujimoto, Ippei Iizuka, Saori Morisawa, Akihiro Nakamura, Nobuyuki Honma, Yoshiyuki Suzuki, Yosuke Shida, Wataru Ogasawara, Nobutada Tanaka, Yasumitsu Sakamoto, Takamasa Nonaka
Dipeptidyl aminopeptidase IV (DAP IV or DPP IV) from Pseudoxanthomonas mexicana WO24 (PmDAP IV) preferentially cleaves substrate peptides with Pro or Ala at the P1 position [NH2-P2-P1(Pro/Ala)-P1'-P2'…]. For crystallographic studies, the periplasmic form of PmDAP IV was overproduced in Escherichia coli, purified and crystallized in complex with the tripeptide Lys-Pro-Tyr using the hanging-drop vapour-diffusion method. Kinetic parameters of the purified enzyme against a synthetic substrate were also determined...
November 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/29090848/evidence-for-a-conserved-microbiota-across-the-different-developmental-stages-of-plodia-interpunctella
#13
Valeria Mereghetti, Bessem Chouaia, Lidia Limonta, Daria Patrizia Locatelli, Matteo Montagna
Diversity and composition of lepidopteran microbiotas are poorly investigated, especially across the different developmental stages. To improve this knowledge, we characterize the microbiota among different developmental stages of the Indian meal moth, Plodia interpunctella, which is considered one of the major pest of commodities worldwide. Using culture-independent approach based on Illumina 16S rRNA gene sequencing we characterized the microbiota of four developmental stages: eggs, first and last instar larvae and adult...
November 1, 2017: Insect Science
https://www.readbyqxmd.com/read/29079625/bioinformatics-analysis-and-characterization-of-highly-efficient-polyvinyl-alcohol-pva-degrading-enzymes-from-the-novel-pva-degrader-stenotrophomonas-rhizophila-ql-p4
#14
Yahong Wei, Jing Fu, Jianying Wu, Xinmiao Jia, Yunheng Zhou, Cuidan Li, Mengxing Dong, Shanshan Wang, Ju Zhang, Fei Chen
Polyvinyl alcohol (PVA) is used widely in industry, and associated environmental pollution is a serious problem. Herein, we report a novel, efficient PVA degrader, Stenotrophomonas rhizophila QL-P4, isolated from fallen leaves from virgin forest in the Qinling Mountains. The complete genome was obtained using single-molecule real-time (SMRT) technology and corrected using Illumina sequencing. Bioinformatics analysis revealed eight PVA/OVA (vinyl alcohol oligomer)-degrading genes. Of these, seven genes were predicted to be involved in the classical intracellular PVA/OVA degradation pathway, and one (BAY15_3292) was identified as a novel PVA oxidase...
October 27, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29079619/identification-of-heterotrophic-zinc-mobilization-processes-among-bacterial-strains-isolated-from-wheat-rhizosphere-triticum-aestivum-l
#15
Benjamin Costerousse, Laurie Schönholzer-Mauclaire, Emmanuel Frossard, Cécile Thonar
Soil and plant inoculation with heterotrophic Zinc Solubilizing Bacteria (ZSB) is considered as a promising approach for increasing zinc (Zn) phytoavailability and enhance crop growth and nutritional quality. It is nevertheless necessary to understand the underlying bacterial solubilization processes in order to predict their repeatability in inoculation strategies. Acidification via gluconic acid production remains the most reported process. In this study, wheat rhizosphere soil serial dilutions were plated onto several solid microbiological media supplemented with scarcely soluble Zn oxide (ZnO) and 115 putative Zn solubilizing isolates were directly detected based on the formation of solubilization halo around the colonies...
October 27, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29077064/stenotrophomonas-maltophilia-a-gram-negative-bacterium-useful-for-transformations-of-flavanone-and-chalcone
#16
Edyta Kostrzewa-Susłow, Monika Dymarska, Urszula Guzik, Danuta Wojcieszyńska, Tomasz Janeczko
A group of flavones, isoflavones, flavanones, and chalcones was subjected to small-scale biotransformation studies with the Gram-negative Stenotrophomonas maltophilia KB2 strain in order to evaluate the capability of this strain to transform flavonoid compounds and to investigate the relationship between compound structure and transformation type. The tested strain transformed flavanones and chalcones. The main type of transformation of compounds with a flavanone moiety was central heterocyclic C ring cleavage, leading to chalcone and dihydrochalcone structures, whereas chalcones underwent reduction to dihydrochalcones and cyclisation to a benzo-γ-pyrone moiety...
October 27, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29075620/the-influence-of-proton-pump-inhibitors-on-the-fecal-microbiome-of-infants-with-gastroesophageal-reflux-a-prospective-longitudinal-interventional-study
#17
Christoph Castellani, Georg Singer, Karl Kashofer, Andrea Huber-Zeyringer, Christina Flucher, Margarita Kaiser, Holger Till
Proton pump inhibitors (PPIs) are the standard therapy for gastroesophageal reflux disease. In adults, PPI treatment is associated with Clostridium difficile infections (CDI). In contrast to adults the microbiome of infants develops from sterility at birth toward an adult-like profile in the first years of life. The effect of PPIs on this developing microbiome has never been studied. The aim of the present study was to determine the effect of oral PPIs on the fecal microbiome in infants with gastroesophageal reflux disease (GERD)...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29062306/the-pgpr-stenotrophomonas-maltophilia-sbp-9-augments-resistance-against-biotic-and-abiotic-stress-in-wheat-plants
#18
Rajnish P Singh, Prabhat N Jha
Certain plant growth promoting bacteria have ability to ameliorate abiotic and/or biotic stressors, which can be exploited to enhance plant growth and productivity of the plants under stress conditions. Therefore, the present study aimed to examine the role of a rhizospheric bacterial isolate SBP-9 isolated from Sorghum bicolor (i) in promoting the wheat plant growth under salinity stress, and (ii) in enhancing the defense response in wheat against fungal pathogen "Fusarium graminearum." The test isolate possessed plant growth promoting (PGP) traits including ACC deaminase (ACCD), gibberellic acid, indole acetic acid (IAA), siderophore, and inorganic phosphate solubilization...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29059308/potential-of-high-dose-cefepime-tazobactam-against-multiresistant-gram-negative-pathogens
#19
David M Livermore, Shazad Mushtaq, Marina Warner, Simon J Turner, Neil Woodford
Background: Early β-lactamase inhibitors were combined with established penicillins, but different combinations may be more appropriate to counter current β-lactamase threats, with development facilitated by the US Generating Antibiotic Incentives Now (GAIN) Act. Cefepime/tazobactam is especially attractive, combining an AmpC-stable cephalosporin with a clinically established inhibitor, active against ESBLs and suitable for high-dose administration. Methods: Organisms (n = 563) were clinical isolates submitted to the UK national reference laboratory...
October 20, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29054663/characterization-of-bacterial-diversity-in-contaminated-groundwater-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#20
Misty S Martin, Inês C Santos, Doug D Carlton, Paula Stigler-Granados, Zacariah L Hildenbrand, Kevin A Schug
Groundwater is a major source for drinking water in the United States, and therefore, its quality and quantity is of extreme importance. One major concern that has emerged is the possible contamination of groundwater due to the unconventional oil and gas extraction activities. As such, the impacts of exogenous contaminants on microbial ecology is an area to be explored to understand what are the chemical and physical conditions that allow the proliferation of pathogenic bacteria and to find alternatives for water treatment by identifying organic-degrading bacteria...
October 17, 2017: Science of the Total Environment
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