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https://www.readbyqxmd.com/read/28706609/distribution-of-class-i-integron-and-smqnr-resistance-gene-among-stenotrophomonas-maltophilia-isolated-from-clinical-samples-in-iran
#1
Mohammadali Malekan, Bahman Tabaraie, Ladan Akhoundtabar, Parviz Afrough, Ava Behrouzi
BACKGROUND: Stenotrophomonas maltophilia (S. maltophilia) is a multiple-antibiotic-resistant opportunistic pathogen that is being isolated with increasing frequency from patients with health-care-associated infections. S. maltophilia is inherently resistant to most of the available antimicrobial agents. Spread of resistant strains has been attributed, in part, to class I integrons. In vitro susceptibility studies have shown trimethoprim-sulfamethoxazole and new floroquinolones as two important agents with activity against these organisms...
July 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28701159/bacterial-bloodstream-infections-in-a-tertiary-infectious-diseases-hospital-in-northern-vietnam-aetiology-drug-resistance-and-treatment-outcome
#2
Vu Quoc Dat, Hieu Ngoc Vu, Hung Nguyen The, Hoa Thi Nguyen, Long Bao Hoang, Dung Vu Tien Viet, Chi Linh Bui, Kinh Van Nguyen, Trung Vu Nguyen, Dao Tuyet Trinh, Alessandro Torre, H Rogier van Doorn, Behzad Nadjm, Heiman F L Wertheim
BACKGROUND: Bloodstream infections (BSIs) are associated with high morbidity and mortality worldwide. However their aetiology, antimicrobial susceptibilities and associated outcomes differ between developed and developing countries. Systematic data from Vietnam are scarce. Here we present aetiologic data on BSI in adults admitted to a large tertiary referral hospital for infectious diseases in Hanoi, Vietnam. METHODS: A retrospective study was conducted at the National Hospital for Tropical Diseases between January 2011 and December 2013...
July 12, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28689013/complete-genome-sequence-of-stenotrophomonas-sp-kctc-12332-a-biotechnological-potential-bacterium
#3
Ha Taek Kwon, Eun Hyuk Jang, Seung Kyun Na, A Ram Shin, Augustine Yonghwi Kim, Young Min Chi, Hyun Park
Hydroxy fatty acids are used in various industries due to their availability, and in particular, Stenotrophomonas sp. has been regarded as a potential candidate for biotechnological applications, including biotransformation that hydrate unsaturated fatty acids into their derivatives. Here we complete the genome sequence of Stenotrophomonas sp. KCTC 12332 which has a size of 4,541,594bp (G+C content of 63.83%) with 3790 coding DNA sequences (CDSs), 67 tRNA and 3 rRNA operons. The genome contains gene encoding oleate hydratase that can convert oleic acid into 10-hydroxyoctadecanoic acid...
July 6, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28688829/risk-factors-for-hospital-acquisition-of-trimethoprim-sulfamethoxazole-resistant-stenotrophomonas-maltophilia-in-adults-a-matched-case-control-study
#4
Ching-Hsun Wang, Jung-Chung Lin, Feng-Yee Chang, Ching-Mei Yu, Wei-San Lin, Kuo-Ming Yeh
BACKGROUND/PURPOSE: The emergence of trimethoprim-sulfamethoxazole resistant Stenotrophomonas maltophilia (TSRSM) represents a serious threat to patients. The aim of current study was to identify risk factors associated with hospital-acquired TSRSM occurrence in adult inpatients. METHODS: We conducted a matched case-control study in Tri-Service General Hospital, Taipei, Taiwan. From January 2014 through June 2015, case patients with TSRSM and control patients with trimethoprim-sulfamethoxazole susceptible S...
June 28, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28681302/hydrocarbon-degradation-potential-and-plant-growth-promoting-activity-of-culturable-endophytic-bacteria-of-lotus-corniculatus-and-oenothera-biennis-from-a-long-term-polluted-site
#5
Małgorzata Pawlik, Barbara Cania, Sofie Thijs, Jaco Vangronsveld, Zofia Piotrowska-Seget
Many endophytic bacteria exert beneficial effects on their host, but still little is known about the bacteria associated with plants growing in areas heavily polluted by hydrocarbons. The aim of the study was characterization of culturable hydrocarbon-degrading endophytic bacteria associated with Lotus corniculatus L. and Oenothera biennis L. collected in long-term petroleum hydrocarbon-polluted site using culture-dependent and molecular approaches. A total of 26 hydrocarbon-degrading endophytes from these plants were isolated...
July 6, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28680041/bsmr-degrades-c-di-gmp-to-modulate-biofilm-formation-of-nosocomial-pathogen-stenotrophomonas-maltophilia
#6
Wei Liu, Xiu-Qi Tian, Jin-Wei Wei, Li-Li Ding, Wei Qian, Zhong Liu, Fang-Fang Wang
c-di-GMP is a cellular second messenger that regulates diverse bacterial processes, including swimming, biofilm formation and virulence. However, in Stenotrophomonas maltophilia, a nosocomial pathogen that frequently infects immunodeficient or immunoincompetent patients, the regulatory function of c-di-GMP remains unclear. Here we show that BsmR is a negative regulator of biofilm development that degrades c-di-GMP through its EAL domain. Increasing BsmR expression resulted in significant increase in bacterial swimming and decrease in cell aggregation...
July 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28674688/d-bmap18-antimicrobial-peptide-is-active-in-vitro-resists-to-pulmonary-proteases-but-loses-its-activity-in-a-murine-model-of-pseudomonas-aeruginosa-lung-infection
#7
Mario Mardirossian, Arianna Pompilio, Margherita Degasperi, Giulia Runti, Sabrina Pacor, Giovanni Di Bonaventura, Marco Scocchi
The spread of antibiotic resistant-pathogens is driving the search for new antimicrobial compounds. Pulmonary infections experienced by cystic fibrosis (CF) patients are a dramatic example of this health-care emergency. Antimicrobial peptides could answer the need for new antibiotics but translating them from basic research to the clinic is a challenge. We have previously evaluated the potential of the small membranolytic peptide BMAP-18 to treat CF-related infections, discovering that while this molecule had a good activity in vitro it was not active in vivo because of its rapid degradation by pulmonary proteases...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/28669293/degradation-of-polyvinyl-alcohol-by-a-novel-bacterial-strain-stenotrophomonas-sp-sa21
#8
Munzer Ullah, Cai-Hong Weng, Hui Li, Shi-Wei Sun, Hong Zhang, Ai-Huan Song, Hu Zhu
In this study, PVA-degrading bacteria were screened from oil sludge using PVA as a sole source of carbon in the culture medium. A novel strain, SA21, was obtained and identified as a member of the Stenotrophomonas genus based on the analysis of a partial 16S rDNA nucleotide sequence, morphological and biochemical characteristics, and phylogenetic analysis. This Stenotrophomonas isolate had not previously been reported as a PVA-degrading bacterium. Stenotrophomonas sp. strain SA21 degraded 90% of the PVA present in culture medium after 4 days...
July 3, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28669074/a-comparative-study-of-utilization-of-single-and-mixed-phenolic-compounds-by-individual-and-mixed-culture
#9
Monisha Tyagaturu Renukaprasad, Mukram Ismailsab, Pooja V Reddy, Kirankumar Baburao, Anand S Nayak, T B Karegoudar
Three bacterial strains; Pseudomonas sp. TRMK1, Stenotrophomonas sp. TRMK2 and Xanthomonas sp. TRMK3 were isolated from agro-industrial waste by enrichment culture technique that are capable of utilizing phenolic acids as sole source of carbon and energy. These strains were found to utilize p-coumaric, ferulic and caffeic acid. The individual strains utilized 5 mM of mixed phenolic acids within 20 h of incubation. The bacterial consortium composing these strains was prepared and studied the efficient degradation of phenolic compounds...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28669016/co-infection-of-pseudomonas-aeruginosa-and-stenotrophomonas-maltophilia-in-hospitalised-pneumonia-patients-has-a-synergic-and-significant-impact-on-clinical-outcomes
#10
C Yin, W Yang, J Meng, Y Lv, J Wang, B Huang
Ventilator-acquired pneumonia and hospital community-acquired pneumonia are frequently caused by Gram-negative and -positive bacteria. We noted that pneumonia patients with co-infection of Pseudomonas aeruginosa and Stenotrophomonas maltophilia had a poor clinical outcome. To verify this, we retrospectively reviewed pneumonia cases at Hebei General Hospital from 2010 to 2015. These cases were grouped into four categories: (1) co-infection with P. aeruginosa and S. maltophilia, (2) infection with P. aeruginosa, (3) infection with S...
July 1, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28666755/activity-of-innate-antimicrobial-peptides-and-ivacaftor-against-clinical-cystic-fibrosis-respiratory-pathogens
#11
Joanna E Payne, Alice V Dubois, Rebecca J Ingram, Sinead Weldon, Clifford C Taggart, J Stuart Elborn, Michael M Tunney
There is a clear need for new antimicrobials to improve current treatment of chronic lung infection in people with cystic fibrosis (CF). This study determined the activity of antimicrobial peptides (AMPs) and ivacaftor, a novel CF transmembrane regulator potentiator for treatment of CF. Antimicrobial activity of AMPs (LL37, Human β-Defensins [HβD] 1-4 and SLPI) and ivacaftor against clinical respiratory isolates (Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus spp., Achromobacter spp. and Stenotrophomonas maltophilia) were determined using radial diffusion and time-kills assays, respectively...
June 27, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28652234/meropenem-vaborbactam-tested-against-contemporary-gram-negative-isolates-collected-worldwide-during-2014-including-carbapenem-resistant-kpc-producing-multidrug-resistant-and-extensively-drug-resistant-enterobacteriaceae
#12
Mariana Castanheira, Michael D Huband, Rodrigo E Mendes, Robert K Flamm
We evaluated the activity of meropenem-vaborbactam against contemporary non-fastidious gram-negative clinical isolates, including resistant phenotypes and carbapenemase genotypes of Enterobacteriaceae Meropenem-vaborbactam (inhibitor at 8 μg/ml) and comparators were susceptibility tested using reference broth microdilution methods against 14,304 gram-negative clinical isolates collected worldwide during 2014. Carbapenemase encoding genes were screened by PCR/sequencing. Meropenem-vaborbactam (MIC50/90, ≤0...
June 26, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28648289/enteric-bacteria-of-food-ice-and-their-survival-in-alcoholic-beverages-and-soft-drinks
#13
Raimondo Gaglio, Nicola Francesca, Rosalia Di Gerlando, Jennifer Mahony, Simone De Martino, Carlo Stucchi, Giancarlo Moschetti, Luca Settanni
This study aimed to evaluate the levels of enteric bacteria in ice cubes produced in different environments (home-made, prepared in bars and pubs with ice machines and produced in industrial plants) and to determine their survival in different alcoholic beverages and soft drinks. Members of the Enterobacteriaceae family were found in almost all samples analysed. All industrial and the majority of home-made samples did not contain coliforms. Enterococci were not identified in domestic samples while they were detected in two industrial and three bar/pub samples...
October 2017: Food Microbiology
https://www.readbyqxmd.com/read/28647881/alterations-in-soil-microbial-communities-caused-by-treatments-with-penicillin-or-neomycin
#14
Qichun Zhang, Ouyang Kang, Salma Jabeen, Warren A Dick
Antibiotic residues in soils can lead to serious health risk and ecological hazards. In this study, the effects of penicillin and neomycin, two antibiotics widely used in animal production, were investigated on soil bacterial communities. Changes in the community structure were monitored using three 16S ribosomal DNA (rDNA) polymerase chain reaction-based approaches, including denaturing gradient gel electrophoresis (DGGE), amplified rDNA restriction analysis (ARDRA), and terminal-restriction fragment length polymorphism (T-RFLP) analysis...
June 25, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28642971/high-diversity-of-bacterial-communities-in-developmental-stages-of-bactrocera-carambolae-insecta-tephritidae-revealed-by-illumina-miseq-sequencing-of-16s-rrna-gene
#15
Hoi-Sen Yong, Sze-Looi Song, Kah-Ooi Chua, Phaik-Eem Lim
Bactrocera carambolae is a highly polyphagous fruit pest of agricultural importance. This study reports the bacterial communities associated with the developmental stages of B. carambolae. The microbiota of the developmental stages were investigated by targeted 16S rRNA gene (V3-V4 region) sequencing using the Illumina MiSeq. At 97% similarity, there were 19 bacterial phyla and unassigned bacteria, comprising 39 classes, 86 orders, 159 families and 311 genera. The bacterial composition varied among the specimens of developmental stage and across developmental stages as well as exuviae...
June 23, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28630181/in-vitro-activity-of-the-siderophore-cephalosporin-cefiderocol-against-a-recent-collection-of-clinically-relevant-gram-negative-bacilli-from-north-america-and-europe-including-carbapenem-non-susceptible-isolates-the-sidero-wt-2014-study
#16
Meredith A Hackel, Masakatsu Tsuji, Yoshinori Yamano, Roger Echols, James A Karlowsky, Daniel F Sahm
Cefiderocol (formerly S-649266) is an investigational siderophore cephalosporin. Iron-depleted cation-adjusted Mueller-Hinton broth (ID-CAMHB) was prepared according to the Clinical and Laboratory Standards Institute (CLSI) protocol and used to perform broth microdilution testing of cefiderocol against a 2014-2015 collection of clinical isolates of gram-negative bacilli from North America (n=4,239) and Europe (n=4,966). The concentration of cefiderocol inhibiting 90% of isolates tested (MIC90) was: 0.5 (North America; n=3,007) and 1 μg/ml (Europe; n=3,080) for all isolates of Enterobacteriaceae; 1 (North America; n=30) and 4 μg/ml (Europe; n=139) for meropenem-non-susceptible (MIC ≥2 μg/ml) isolates of Enterobacteriaceae; 0...
June 19, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28629970/variation-analysis-of-bacterial-polyhydroxyalkanoates-production-using-saturated-and-unsaturated-hydrocarbons
#17
Saiqa Tufail, Sajida Munir, Nazia Jamil
Polyhydroxyalkanoates (PHA) are efficient, renewable and environment friendly polymeric esters. These polymers are synthesized by a variety of microbes under stress conditions. This study was carried out to check the suitability of waste frying oil in comparison to other oils for economical bioplastic production. Six bacterial strains were isolated and identified as Bacillus cereus (KF270349), Klebsiella pneumoniae (KF270350), Bacillus subtilis (KF270351), Brevibacterium halotolerance (KF270352), Pseudomonas aeruginosa (KF270353), and Stenotrophomonas rhizoposid (KF270354) by ribotyping...
May 29, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28629502/transfer-of-pseudomonas-pictorum-gray-and-thornton-1928-to-genus-stenotrophomonas-as-stenotrophomonas-pictorum-comb-nov-and-emended-description-of-the-genus-stenotrophomonas
#18
Aboubakar Sidiki Ouattara, Jean Le Mer, Manon Joseph, Hervé Macarie
A polyphasic taxonomic approach including analysis of phenotypic, physiological and genotypic characteristics, 16S rRNA gene sequence and DNA-DNA hybridization analysis was used to determine the most consistent affiliation of Pseudomonas pictorum. Pseudomonas pictorum ATCC 23328T exhibited phenotypic traits of members of the genus Stenotrophomonas including cellular fatty acid composition, quinone and limited range of substrates that could be used. Antibiotic susceptibility and physiological characteristics were determined...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28622646/enhanced-degradation-of-chlorpyrifos-in-rice-oryza-sativa-l-by-five-strains-of-endophytic-bacteria-and-their-plant-growth-promotional-ability
#19
Fayun Feng, Jing Ge, Yisong Li, Shuang He, Jianfeng Zhong, Xianjing Liu, Xiangyang Yu
Endophytic bacteria reside in plant tissues, such as roots, stems, leaves and seeds. Most of them can stimulate plant growth or alleviate phytotoxicity of pollutants. There are handful species with dual functions stimulating plant growth and degrading pollutants have been reported. Five endophytic bacteria were isolated from chlorpyrifos (CP) treated rice plants and identified as Pseudomonas aeruginosa strain RRA, Bacillus megaterium strain RRB, Sphingobacterium siyangensis strain RSA, Stenotrophomonas pavanii strain RSB and Curtobacterium plantarum strain RSC according to morphological characteristics, physiological and biochemical tests, and 16S rDNA phylogeny...
October 2017: Chemosphere
https://www.readbyqxmd.com/read/28618083/low-abundant-species-facilitates-specific-spatial-organization-that-promotes-multispecies-biofilm-formation
#20
Wenzheng Liu, Jakob Russel, Henriette L Røder, Jonas S Madsen, Mette Burmølle, Søren J Sørensen
Microorganisms frequently co-exist in matrix-embedded multispecies biofilms. Within biofilms, interspecies interactions influence the spatial organization of member species, which likely play an important role in shaping the development, structure and function of these communities. Here, a reproducible four-species biofilm, composed of Stenotrophomonas rhizophila, Xanthomonas retroflexus, Microbacterium oxydans and Paenibacillus amylolyticus, was established to study the importance of individual species spatial organization during multispecies biofilm development...
June 15, 2017: Environmental Microbiology
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