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https://www.readbyqxmd.com/read/28642971/high-diversity-of-bacterial-communities-in-developmental-stages-of-bactrocera-carambolae-insecta-tephritidae-revealed-by-illumina-miseq-sequencing-of-16s-rrna-gene
#1
Hoi-Sen Yong, Sze-Looi Song, Kah-Ooi Chua, Phaik-Eem Lim
Bactrocera carambolae is a highly polyphagous fruit pest of agricultural importance. This study reports the bacterial communities associated with the developmental stages of B. carambolae. The microbiota of the developmental stages were investigated by targeted 16S rRNA gene (V3-V4 region) sequencing using the Illumina MiSeq. At 97% similarity, there were 19 bacterial phyla and unassigned bacteria, comprising 39 classes, 86 orders, 159 families and 311 genera. The bacterial composition varied among the specimens of developmental stage and across developmental stages as well as exuviae...
June 23, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28630181/in-vitro-activity-of-the-siderophore-cephalosporin-cefiderocol-against-a-recent-collection-of-clinically-relevant-gram-negative-bacilli-from-north-america-and-europe-including-carbapenem-non-susceptible-isolates-the-sidero-wt-2014-study
#2
Meredith A Hackel, Masakatsu Tsuji, Yoshinori Yamano, Roger Echols, James A Karlowsky, Daniel F Sahm
Cefiderocol (formerly S-649266) is an investigational siderophore cephalosporin. Iron-depleted cation-adjusted Mueller-Hinton broth (ID-CAMHB) was prepared according to the Clinical and Laboratory Standards Institute (CLSI) protocol and used to perform broth microdilution testing of cefiderocol against a 2014-2015 collection of clinical isolates of gram-negative bacilli from North America (n=4,239) and Europe (n=4,966). The concentration of cefiderocol inhibiting 90% of isolates tested (MIC90) was: 0.5 (North America; n=3,007) and 1 μg/ml (Europe; n=3,080) for all isolates of Enterobacteriaceae; 1 (North America; n=30) and 4 μg/ml (Europe; n=139) for meropenem-non-susceptible (MIC ≥2 μg/ml) isolates of Enterobacteriaceae; 0...
June 19, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28629970/variation-analysis-of-bacterial-polyhydroxyalkanoates-production-using-saturated-and-unsaturated-hydrocarbons
#3
Saiqa Tufail, Sajida Munir, Nazia Jamil
Polyhydroxyalkanoates (PHA) are efficient, renewable and environment friendly polymeric esters. These polymers are synthesized by a variety of microbes under stress conditions. This study was carried out to check the suitability of waste frying oil in comparison to other oils for economical bioplastic production. Six bacterial strains were isolated and identified as Bacillus cereus (KF270349), Klebsiella pneumoniae (KF270350), Bacillus subtilis (KF270351), Brevibacterium halotolerance (KF270352), Pseudomonas aeruginosa (KF270353), and Stenotrophomonas rhizoposid (KF270354) by ribotyping...
May 29, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28629502/transfer-of-pseudomonas-pictorum-gray-and-thornton-1928-to-genus-stenotrophomonas-as-stenotrophomonas-pictorum-comb-nov-and-emended-description-of-the-genus-stenotrophomonas
#4
Aboubakar Sidiki Ouattara, Jean Le Mer, Manon Joseph, Hervé Macarie
A polyphasic taxonomic approach including analysis of phenotypic, physiological and genotypic characteristics, 16S rRNA gene sequence and DNA-DNA hybridization analysis was used to determine the most consistent affiliation of Pseudomonas pictorum. Pseudomonas pictorum ATCC 23328T exhibited phenotypic traits of members of the genus Stenotrophomonas including cellular fatty acid composition, quinone and limited range of substrates that could be used. Antibiotic susceptibility and physiological characteristics were determined...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28622646/enhanced-degradation-of-chlorpyrifos-in-rice-oryzasativa-l-by-five-strains-of-endophytic-bacteria-and-their-plant-growth-promotional-ability
#5
Fayun Feng, Jing Ge, Yisong Li, Shuang He, Jianfeng Zhong, Xianjing Liu, Xiangyang Yu
Endophytic bacteria reside in plant tissues, such as roots, stems, leaves and seeds. Most of them can stimulate plant growth or alleviate phytotoxicity of pollutants. There are handful species with dual functions stimulating plant growth and degrading pollutants have been reported. Five endophytic bacteria were isolated from chlorpyrifos (CP) treated rice plants and identified as Pseudomonas aeruginosa strain RRA, Bacillus megaterium strain RRB, Sphingobacterium siyangensis strain RSA, Stenotrophomonas pavanii strain RSB and Curtobacterium plantarum strain RSC according to morphological characteristics, physiological and biochemical tests, and 16S rDNA phylogeny...
June 3, 2017: Chemosphere
https://www.readbyqxmd.com/read/28618083/low-abundant-species-facilitates-specific-spatial-organisation-that-promotes-multispecies-biofilm-formation
#6
Wenzheng Liu, Jakob Russel, Henriette L Røder, Jonas S Madsen, Mette Burmølle, Søren J Sørensen
Microorganisms frequently co-exist in matrix-embedded multispecies biofilms. Within biofilms, interspecies interactions influence the spatial organisation of member species, which likely play an important role in shaping the development, structure and function of these communities. Here, a reproducible four-species biofilm, composed of Stenotrophomonas rhizophila, Xanthomonas retroflexus, Microbacterium oxydans and Paenibacillus amylolyticus, was established to study the importance of individual species spatial organisation during multispecies biofilm development...
June 15, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28602707/distribution-and-characterization-of-stenotrophomonas-maltophilia-isolates-from-environmental-and-clinical-samples-in-thailand
#7
Panida Paopradit, Kanchana Srinitiwarawong, Natnicha Ingviya, Kamonnut Singkhamanan, Varaporn Vuddhakul
BACKGROUND: Stenotrophomonas maltophilia has emerged as an important opportunistic pathogen especially in immunocompromised hosts suffering from malignancy and prolonged hospitalization. Information of this bacterium in Thailand has not been elucidated. AIMS: To investigate phenotype and genotype of environmental and clinical isolates of S. maltophilia in Songklanagarind hospital, southern Thailand. METHODS: Isolates of S. maltophilia from various environmental sources on 3 hospital wards and clinical samples from 7 wards were collected...
June 8, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28599639/seasonality-of-acquisition-of-respiratory-bacterial-pathogens-in-young-children-with-cystic-fibrosis
#8
Kevin J Psoter, Anneclaire J De Roos, Jon Wakefield, Jonathan D Mayer, Margaret Rosenfeld
BACKGROUND: Seasonal variations are often observed for respiratory tract infections; however, limited information is available regarding seasonal patterns of acquisition of common cystic fibrosis (CF)-related respiratory pathogens. We previously reported differential seasonal acquisition of Pseudomonas aeruginosa in young children with CF and no such variation for methicillin-susceptible Staphylococcus aureus acquisition. The purpose of this study was to describe and compare the seasonal incidence of acquisition of other respiratory bacterial pathogens in young children with CF...
June 9, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28588123/a-genoproteomic-approach-to-detect-peptide-markers-of-bacterial-respiratory-pathogens
#9
Honghui Wang, Steven K Drake, Chen Yong, Marjan Gucek, Matthew A Lyes, Avi Z Rosenberg, Erik Soderblom, M Arthur Moseley, John P Dekker, Anthony F Suffredini
BACKGROUND: Rapid identification of respiratory pathogens may facilitate targeted antimicrobial therapy. Direct identification of bacteria in bronchoalveolar lavage (BAL) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is confounded by interfering substances. We describe a method to identify unique peptide markers of 5 gram-negative bacteria by liquid chromatography-tandem mass spectrometry (LC-MS/MS) for direct pathogen identification in BAL. METHODS: In silico translation and digestion were performed on 14-25 whole genomes representing strains of Acinetobacter baumannii, Moraxella catarrhalis, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Klebsiella pneumoniae...
June 6, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28569336/molecular-epidemiology-and-characteristics-of-16-cases-with-stenotrophomonas-maltophilia-bacteraemia-in-pediatric-intensive-care-units
#10
M Motamedifar, H Heidari, M Yasemi, H Sedigh Ebrahim-Saraie
BACKGROUND: Recently, Stenotrophomonas maltophilia has increasingly been reported as an important nosocomial opportunistic pathogen. Limited therapeutic options of S. maltophilia infections demand early identification and knowledge about the probable risk factors for controlling its spread. STUDY DESIGN: The present study aimed to investigate the risk factors and trend of antibiotic susceptibility, along with genetic analysis in bacteraemia cases at pediatric intensive care units (PICUs)...
July 2017: Annali di Igiene: Medicina Preventiva e di Comunità
https://www.readbyqxmd.com/read/28567625/biodegradation-of-%C3%AE-endosulfan-via-hydrolysis-pathway-by-stenotrophomonas-maltophilia-og2
#11
Murat Ozdal, Ozlem Gur Ozdal, Omer Faruk Algur, Esabi Basaran Kurbanoglu
Stenotrophomonas maltophilia OG2 was isolated from the intestine of cockroaches that was collected from a cow barn contaminated some pesticides belong to pyrethroid and organochlorine groups. OG2 was able to degrade α-endosulfan in non sulfur medium (NSM) as a sole sulfur source for growth within 10 days of incubation. The effects of some growth parameters on endosulfan biodegradation by OG2 was studied and found that the biodegradation was significantly affected by the endosulfan concentrations, pH and temperature...
June 2017: 3 Biotech
https://www.readbyqxmd.com/read/28561275/screening-for-biosurfactant-production-by-2-4-6-trinitrotoluene-transforming-bacteria
#12
H Avila-Arias, H Avellaneda, V Garzón, G Rodríguez, Z Arbeli, E Garcia-Bonilla, S Villegas-Plazas, F Roldan
AIMS: Isolate and identify TNT-transforming cultures from explosive-contaminated soils with the ability to produce biosurfactants. METHODS AND RESULTS: Bacteria (pure and mixed cultures) were selected based on their ability to transform TNT in minimum media with TNT as the sole nitrogen source and an additional carbon source. TNT-transforming bacteria were identified by 16S rRNA gene sequencing. TNT transformation rates were significantly lower when no additional carbon or nitrogen source were added...
May 31, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28520867/activity-of-ceftolozane-tazobactam-against-surveillance-and-problem-enterobacteriaceae-pseudomonas-aeruginosa-and-non-fermenters-from-the-british-isles
#13
David M Livermore, Shazad Mushtaq, Daniele Meunier, Katie L Hopkins, Robert Hill, Rachael Adkin, Aiysha Chaudhry, Rachel Pike, Peter Staves, Neil Woodford
Background: We assessed the activity of ceftolozane/tazobactam against consecutive isolates collected in the BSAC Bacteraemia Surveillance from 2011 to 2015 and against 'problem' isolates sent to the UK national reference laboratory from July 2015, when routine testing began. Methods: Susceptibility testing was by BSAC agar dilution with resistance mechanisms identified by PCR and interpretive reading. Results: Data were reviewed for 6080 BSAC surveillance isolates and 5473 referred organisms...
May 16, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28514306/unusual-case-of-rapid-growing-intraabdominal-abscess-caused-by-stenotrophomonas-maltophilia-after-laparoscopic-appendectomy-due-to-perforated-appendicitis-a-case-report
#14
Dae Ro Lim, Jung Cheol Kuk, Taehyung Kim, Eung Jin Shin
INTRODUCTION: An intraabdominal abscess due to Stenotrophomonas maltophilia (S maltophilia) infection is a very rare clinical manifestation. S maltophilia is a glucose nonfermentative, aerobic, gram-negative, mobile, and biofilm-forming bacterium. It is an opportunistic pathogen and uncommon cause of infection. Respiratory tract infections (pneumonia) and bloodstream infections (bacteremia) are the most common clinical manifestations of S maltophilia infection. CONCLUSIONS: This case report describes an unusual case of a rapidly growing, extremely large intraabdominal abscess (within 1 week during antibiotic therapy), which was detected 2 weeks after a laparoscopic appendectomy was performed for perforated appendicitis and was caused by multidrug-resistant S maltophilia infection...
May 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28502480/stenotrophomonas-maltophilia-in-a-university-hospital-of-traditional-chinese-medicine-molecular-epidemiology-and-antimicrobial-resistance
#15
S Zhao, L Yang, H Liu, F Gao
Stenotrophomona maltophilia has emerged as an important opportunistic pathogen that is highly antibiotic resistant. Analysis of antibiotic susceptibilities, drug-resistant gene profiles and molecular typing of S. maltophilia was undertaken in a university hospital of traditional Chinese medicine in East China. Resistance to sulphamethoxazole (SXT) was found to be an indicator of multi-drug resistance. SXT resistance was mediated by sul and dfrA genes in integrons, especially class 1. Some evidence of clonal dissemination was found, indicating the occurrence of cross-transmission of antibiotic-resistant strains within the hospital...
July 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28488744/membrane-vesicle-secretion-and-prophage-induction-in-multidrug-resistant-stenotrophomonas-maltophilia-in-response-to-ciprofloxacin-stress
#16
Simon Devos, Wouter Van Putte, Jolien Vitse, Gonzalez Van Driessche, Stephan Stremersch, Wim Van Den Broek, Koen Raemdonck, Kevin Braeckmans, Henning Stahlberg, Misha Kudryashev, Savvas N Savvides, Bart Devreese
Several bacterial species produce membrane vesicles (MVs) in response to antibiotic stress. However, the biogenesis and role of MVs in bacterial antibiotic resistance mechanisms have remained unclear. Here we studied the effect of the fluoroquinolone ciprofloxacin on MV secretion by Stenotrophomonas maltophilia using a combination of electron microscopy and proteomic approaches. We found that in addition to the classical outer membrane vesicles (OMV), ciprofloxacin-stimulated cultures produced larger vesicles containing both outer and inner membranes termed outer-inner membrane vesicles (OIMV), and that such MVs are enriched with cytosolic proteins...
May 10, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28484160/rapidly-progressive-hemorrhagic-pneumonia-caused-by-stenotrophomonas-maltophilia-occurring-early-after-bone-marrow-transplantation-in-a-patient-with-myelodysplastic-syndrome
#17
Oju Katayama, Tomoya Kitagawa, Takashi Onaka, Akihito Yonezawa
A 44-year-old woman had repeated bacterial infections because of hypoplastic myelodysplastic syndrome; therefore, she underwent allogeneic hematopoietic cell transplantation. Broad spectrum antibiotics were administered because she had bacterial infection and pneumonia 2 weeks before undergoing transplantation. On day19 after transplantation, she suddenly presented with hemoptysis. Stenotrophomonas maltophilia was considered to be the cause of hemorrhagic pneumonia, which required ventilation support, massive transfusion, and catecholamine administration...
2017: [Rinshō Ketsueki] the Japanese Journal of Clinical Hematology
https://www.readbyqxmd.com/read/28473944/antimicrobial-efficacy-of-contact-lens-care-solutions-against-neutrophil-enhanced-bacterial-biofilms
#18
Jorge A Hinojosa, Naiya B Patel, Meifang Zhu, Danielle M Robertson
PURPOSE: Neutrophil-derived extracellular debris has been shown to accelerate bacterial biofilm formation on hydrogel and silicone hydrogel contact lens surfaces compared to lenses inoculated with bacteria alone. The purpose of this study was to evaluate the disinfection efficacy of four standard commercial contact lens cleaning regimens against neutrophil-enhanced bacterial biofilms formed on silicone hydrogel contact lenses. METHODS: Four reference strains were used: Pseudomonas aeruginosa, Serratia marcescens, Stenotrophomonas maltophilia, and Staphylococcus aureus...
April 2017: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/28464580/-analysis-of-the-spectrum-and-resistance-of-pathogen-causing-sepsis-in-patients-with-severe-acute-pancreatitis
#19
H X Ma, L He, S W Cai, X L Xin, H D Shi, L Zhou, X J Shi
Objective: To investigate the characteristics of spectrum and drug resistance of pathogens causing sepsis in patients with severe acute pancreatitis(SAP). Methods: The clinical data of 63 SAP patients with sepsis admitted in Department of Hepatobiliary, People's Liberation Army General Hospital from January 2014 to December 2015 were retrospectively studied. There were 47 males and 16 females, aged from 22 to 73 years, with an average age of (52±11)years. Samples were collected mainly from: (1)pancreatic and peripancreatic necrosis and abdominal drainage; (2)bile; (3) blood or deep venous catheter; (4) sputum and tracheal catheter and thoracic drainage; (5) urine...
May 1, 2017: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
https://www.readbyqxmd.com/read/28460971/microbial-communities-related-to-sensory-attributes-in-korean-fermented-soy-bean-paste-doenjang
#20
Min Jung Kim, Han Sub Kwak, Hee Yeon Jung, Sang Sook Kim
Various microbial communities play a role in generating the distinctive sensory characteristics of the traditional Korean soybean paste, doenjang. The objective of this study was to investigate the relationship between sensory characteristics and microbial communities in traditional doenjang. The results of the descriptive analysis revealed that some traditional doenjang (T1-T11) exhibited distinctive characteristics, such as fish sauce flavor, meju, bitterness, sourness, and saltiness, whereas the modified samples (M1-M3) demonstrated sweetness, umami, and an alcohol odor...
November 2016: Food Research International
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