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https://www.readbyqxmd.com/read/27907871/fluorescent-probe-based-subcellular-distribution-of-cu-ii-ions-in-living-electrotrophs-isolated-from-cu-ii-reduced-biocathodes-of-microbial-fuel-cells
#1
Ye Tao, Hua Xue, Liping Huang, Peng Zhou, Wei Yang, Xie Quan, Jinxiu Yuan
Based on the four indigenous electrotrophs (Stenotrophomonas maltophilia JY1, Citrobacter sp. JY3, Pseudomonas aeruginosa JY5 and Stenotrophomonas sp. JY6) isolated from well adapted Cu(II)-reduced biocathodes of microbial fuel cells (MFCs), a rhodamine based Cu(II) fluorescent probe was used to imaginably and quantitatively track subcellular Cu(II) ions in these electrotrophs. Cathodic electrons led to more Cu(II) ions (14.3-30.1%) in the intracellular sites at operation time of 2-3h with Cu(II) removal rates of 2...
November 22, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27899336/complete-genome-sequence-of-stenotrophomonas-sp-kacc-91585-an-efficient-bacterium-for-unsaturated-fatty-acid-hydration
#2
Kyoung-Rok Kim, Woo-Ri Kang, Deok-Kun Oh
Hydroxy fatty acids (HFAs) such as 10-hydroxystearic acid (10-HSA) and 10-hydroxy-12(Z)-octadecenoic acid (10-HOD), which are similar to ricinoleic acid, are important starting materials and intermediates for the industrial manufacture of many commodities. Stenotrophomonas sp. KACC 91585, which was isolated from lake sediment, is an efficient bacterium for unsaturated fatty acid hydration that produces 10-HSA and 10-HOD from oleic acid and linoleic acid, respectively, with high conversion rates. The complete genome of this strain is 4,541,729bp with 63...
November 27, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27889889/biosurfactant-production-by-the-crude-oil-degrading-stenotrophomonas-sp-b-2-chemical-characterization-biological-activities-and-environmental-applications
#3
Boutheina Gargouri, María Del Mar Contreras, Sonda Ammar, Antonio Segura-Carretero, Mohamed Bouaziz
In this work, biosurfactant-producing microorganisms were isolated from hydrocarbon-contaminated water collected from Tunisian oilfield. After enrichment and isolation, different bacterial strains were preliminary studied for their biosurfactant/bioemulsifier properties when using crude oil as the unique carbon source. In particular, the isolate strain B-2, a Gram-negative, rod-shaped bacterium, efficiently emulsified crude oil. The extracellular biosurfactant product from this strain presented an emulsification activity above 70% and a hydrophobicity of 71%...
November 26, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27889181/brazilian-cerrado-soil-reveals-an-untapped-microbial-potential-for-unpretreated-polyethylene-biodegradation
#4
Julianna Peixoto, Luciano P Silva, Ricardo H Krüger
Discarded PE-based products pose a social and environmental threat because of their recalcitrance to degradation, a consequence of the unique set of PE's physicochemical properties. In this study we isolated nine novel PE-degrading bacteria from plastic debris found in soil of the savanna-like Brazilian Cerrado. These bacterial strains from the genera Comamonas, Delftia, and Stenotrophomonas showed metabolic activity and cellular viability after a 90-day incubation with PE as the sole carbon source. ATR/FTIR indicated that biodegraded PE undergone oxidation, vinylene formation, chain scission, among other chemical changes...
November 14, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/27881616/performance-of-vitek%C3%A2-2-for-antimicrobial-susceptibility-testing-of-acinetobacter-baumannii-pseudomonas-aeruginosa-and-stenotrophomonas-maltophilia-with-vitek%C3%A2-2-2009-fda-and-clsi-m100s-26th-edition-breakpoints
#5
April M Bobenchik, Eszter Deak, Janet A Hindler, Carmen L Charlton, Romney M Humphries
Performance of Vitek 2 AST-GN69 and AST-XN06 cards was compared to Clinical and Laboratory Standards Institute (CLSI) reference broth microdilution (BMD) for 99 isolates of Pseudomonas aeruginosa, 26 Acinetobacter baumannii and 11 Stenotrophomonas maltophilia In total 15 antimicrobials were evaluated, 11 for P. aeruginosa, 14 for A. baumannii, and 2 for S. maltophilia. Categorical agreement (CA) was assessed using both Vitek 2 breakpoints and 2016 CLSI M100S 26(th) edition breakpoints. Essential agreement for P...
November 23, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27870863/characteristics-of-aspergillus-fumigatus-in-association-with-stenotrophomonas-maltophilia-in-an-in-vitro-model-of-mixed-biofilm
#6
Elise Melloul, Stéphanie Luiggi, Leslie Anaïs, Pascal Arné, Jean-Marc Costa, Vincent Fihman, Benoit Briard, Eric Dannaoui, Jacques Guillot, Jean-Winoc Decousser, Anne Beauvais, Françoise Botterel
BACKGROUND: Biofilms are communal structures of microorganisms that have long been associated with a variety of persistent infections poorly responding to conventional antibiotic or antifungal therapy. Aspergillus fumigatus fungus and Stenotrophomonas maltophilia bacteria are examples of the microorganisms that can coexist to form a biofilm especially in the respiratory tract of immunocompromised patients or cystic fibrosis patients. The aim of the present study was to develop and assess an in vitro model of a mixed biofilm associating S...
2016: PloS One
https://www.readbyqxmd.com/read/27861524/factors-associated-with-worse-lung-function-in-cystic-fibrosis-patients-with-persistent-staphylococcus-aureus
#7
Sibylle Junge, Dennis Görlich, Martijn den Reijer, Bärbel Wiedemann, Burkhard Tümmler, Helmut Ellemunter, Angelika Dübbers, Peter Küster, Manfred Ballmann, Cordula Koerner-Rettberg, Jörg Große-Onnebrink, Eberhardt Heuer, Wolfgang Sextro, Jochen G Mainz, Jutta Hammermann, Joachim Riethmüller, Ute Graepler-Mainka, Doris Staab, Bettina Wollschläger, Rüdiger Szczepanski, Antje Schuster, Friedrich-Karl Tegtmeyer, Sivagurunathan Sutharsan, Alexandra Wald, Jerzy-Roch Nofer, Willem van Wamel, Karsten Becker, Georg Peters, Barbara C Kahl
BACKGROUND: Staphylococcus aureus is an important pathogen in cystic fibrosis (CF). However, it is not clear which factors are associated with worse lung function in patients with persistent S. aureus airway cultures. Our main hypothesis was that patients with high S. aureus density in their respiratory specimens would more likely experience worsening of their lung disease than patients with low bacterial loads. METHODS: Therefore, we conducted an observational prospective longitudinal multi-center study and assessed the association between lung function and S...
2016: PloS One
https://www.readbyqxmd.com/read/27853518/metagenomic-analysis-of-medicinal-cannabis-samples-pathogenic-bacteria-toxigenic-fungi-and-beneficial-microbes-grow-in-culture-based-yeast-and-mold-tests
#8
Kevin McKernan, Jessica Spangler, Yvonne Helbert, Ryan C Lynch, Adrian Devitt-Lee, Lei Zhang, Wendell Orphe, Jason Warner, Theodore Foss, Christopher J Hudalla, Matthew Silva, Douglas R Smith
Background: The presence of bacteria and fungi in medicinal or recreational Cannabis poses a potential threat to consumers if those microbes include pathogenic or toxigenic species. This study evaluated two widely used culture-based platforms for total yeast and mold (TYM) testing marketed by 3M Corporation and Biomérieux, in comparison with a quantitative PCR (qPCR) approach marketed by Medicinal Genomics Corporation. Methods: A set of 15 medicinal Cannabis samples were analyzed using 3M and Biomérieux culture-based platforms and by qPCR to quantify microbial DNA...
2016: F1000Research
https://www.readbyqxmd.com/read/27851405/1770-successful-treatment-of-stenotrophomonas-maltophilia-pneumonia-in-a-preterm-neonatal-patient
#9
Pooja Shah, Sabrina Malik
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27848015/biodegradation-of-polycyclic-aromatic-hydrocarbons-by-an-acidophilic-stenotrophomonas-maltophilia-strain-ajh1-isolated-from-a-mineral-mining-site-in-saudi-arabia
#10
P Arulazhagan, K Al-Shekri, Q Huda, J J Godon, J M Basahi, D Jeyakumar
The present study aims at analyzing the degradation of polycyclic aromatic hydrocarbons (PAHs) at acidic conditions (pH = 2) by acidophilic Stenotrophomonas maltophilia strain AJH1 (KU664513). The strain AJH1 was obtained from an enrichment culture obtained from soil samples of mining area in the presence of PAH as sole sources of carbon and energy. Strain AJH1was able to degrade low (anthracene, phenanthrene, naphthalene, fluorene) and high (pyrene, benzo(e)pyrene and benzo(k)fluoranthene) molecular weight PAHs in acidophilic mineral salt medium at pH 2, with removal rates of up to 95% (LMW PAH) and 80% (HMW PAH), respectively...
November 16, 2016: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/27835761/correlation-between-circuital-current-cu-ii-reduction-and-cellular-electron-transfer-in-eab-isolated-from-cu-ii-reduced-biocathodes-of-microbial-fuel-cells
#11
Jingya Shen, Liping Huang, Peng Zhou, Xie Quan, Gianluca Li Puma
The performance of four indigenous electrochemically active bacteria (EAB) (Stenotrophomonas maltophilia JY1, Citrobacter sp. JY3, Pseudomonas aeruginosa JY5 and Stenotrophomonas sp. JY6) was evaluated for Cu(II) reduction on the cathodes of microbial fuel cells (MFCs). These EAB were isolated from well adapted mixed cultures on the MFC cathodes operated for Cu(II) reduction. The relationship between circuital current, Cu(II) reduction rate, and cellular electron transfer processes was investigated from a mechanistic point of view using X-ray photoelectron spectroscopy, scanning electronic microscopy coupled with energy dispersive X-ray spectrometry, linear sweep voltammetry and cyclic voltammetry...
November 5, 2016: Bioelectrochemistry
https://www.readbyqxmd.com/read/27833606/surveillance-of-dihydropteroate-synthase-genes-in-stenotrophomonas-maltophilia-by-lamp-implications-for-infection-control-and-initial-therapy
#12
Jin Zhao, Yubin Xing, Wei Liu, Wentao Ni, Chuanqi Wei, Rui Wang, Yunxi Liu, Youning Liu
Stenotrophomonas maltophilia is a common nosocomial pathogen that causes high morbidity and mortality. Because of its inherent extended antibiotic resistance, therapeutic options for S. maltophilia are limited, and sulfamethoxazole/trimethoprim (SXT) is the only first-line antimicrobial recommended. However, with the spread of dihydropteroate synthase (sul1 and sul2) genes, global emergence of SXT resistance has been reported. There is an urgent need to develop a rapid and sensitive but cost-efficient method to monitor the dissemination of sul genes...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27833412/computed-tomography-findings-of-community-acquired-stenotrophomonas-maltophilia-pneumonia-in-an-immunocompetent-patient-a-case-report
#13
Yoon Ki Cha, Jeung Sook Kim, Seong Yeon Park, Jin Young Oh, Jae Hyun Kwon
Stenotrophomonas maltophilia (S. maltophilia) is a rare, but globally emerging gram-negative multiple-drug-resistant organism usually found in a nosocomial setting in immunocompromised patients. To our best knowledge, computed tomography (CT) features of community-acquired S. maltophilia pneumonia have not been previously reported in an immunocompetent patient. Herein, we presented the CT findings of a previous healthy 56-year-old male with S. maltophilia pneumonia.
November 2016: Korean Journal of Radiology: Official Journal of the Korean Radiological Society
https://www.readbyqxmd.com/read/27827894/phenanthrene-and-pyrene-modify-the-composition-and-structure-of-the-cultivable-endophytic-bacterial-community-in-ryegrass-lolium-multiflorum-lam
#14
Xuezhu Zhu, Li Jin, Kai Sun, Shuang Li, Xuelin Li, Wanting Ling
This study provides new insights into the dynamics of bacterial community structure during phytoremediation. The communities of cultivable autochthonous endophytic bacteria in ryegrass exposed to polycyclic aromatic hydrocarbons (PAHs) were investigated with regard to their potential to biodegrade PAHs. Bacterial counts and 16S rRNA gene sequence were used in the microbiological evaluation. A total of 33 endophytic bacterial strains were isolated from ryegrass plants, which represented 15 different genera and eight different classes, respectively...
November 3, 2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/27822692/genetic-and-biochemical-characterization-of-rhizobacterial-strains-and-their-potential-use-in-combination-with-chelants-for-assisted-phytoremediation
#15
Cicatelli Angela, Guarino Francesco, Baldan Enrico, Castiglione Stefano
Copper and zinc are essential micronutrients in plants but, at high concentrations, they are toxic. Assisted phytoremediation is an emerging "green" technology that aims to improve the efficiency of tolerant species to remove metals from soils through the use of chelants or microorganisms. Rhizobacteria can promote plant growth and tolerance and also affect the mobility, bioavailability, and complexation of metals. A pot experiment was conducted to evaluate the phytoremediation effectiveness of sunflowers cultivated in a Cu- and Zn-spiked soil, in the presence or absence of bacterial consortium and/or chelants...
November 7, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27812800/16s-rrna-gene-based-characterization-of-bacteria-potentially-associated-with-phosphate-and-carbonate-precipitation-from-a-granular-autotrophic-nitrogen-removal-bioreactor
#16
Alejandro Gonzalez-Martinez, Alejandro Rodriguez-Sanchez, María Angustias Rivadeneyra, Almudena Rivadeneyra, Daniel Martin-Ramos, Riku Vahala, Jesús Gonzalez-Lopez
A bench-scale granular autotrophic nitrogen removal bioreactor (completely autotrophic nitrogen removal over nitrite (CANON) system) used for the treatment of synthetic wastewater was analyzed for the identification of microbiota with potential capacity for carbonate and phosphate biomineral formation. 16S ribosomal RNA (rRNA) gene-based studies revealed that different bacterial species found in the granular biomass could trigger the formation of phosphate and calcite minerals in the CANON bioreactor. iTag analysis of the microbial community in the granular biomass with potential ability to precipitate calcium carbonate and hydroxyapatite constituted around 0...
November 3, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27810239/responses-of-earthworms-and-microbial-communities-in-their-guts-to-triclosan
#17
Lili Ma, Yuwei Xie, Zhihua Han, John P Giesy, Xiaowei Zhang
Responses of the earthworm (Eisenia fetida) and compositions of associated microbial communities were determined after exposure to various concentrations of Triclosan (TCS) for 7 d. Concentrations of TCS were greater in intestines than in epidermis of earthworms, which suggested that earthworms accumulate TCS mainly by ingestion rather than by epidermic penetration. Exposure to TCS caused a concentration-dependent increase in activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) and in malondialdehyde (MDA) in E...
October 31, 2016: Chemosphere
https://www.readbyqxmd.com/read/27798695/genotypic-and-phenotypic-characterization-of-stenotrophomonas-maltophilia-strains-from-a-pediatric-tertiary-care-hospital-in-serbia
#18
Haowa Madi, Jovanka Lukić, Zorica Vasiljević, Marjan Biočanin, Milan Kojić, Branko Jovčić, Jelena Lozo
BACKGROUND: Stenotrophomonas maltophilia is an environmental bacterium and an opportunistic pathogen usually associated with healthcare-associated infections, which has recently been recognized as a globally multi-drug resistant organism. The aim of this study was genotyping and physiological characterization of Stenotrophomonas maltophilia isolated in a large, tertiary care pediatric hospital in Belgrade, Serbia, hosting the national reference cystic fibrosis (CF) center for pediatric and adult patients...
2016: PloS One
https://www.readbyqxmd.com/read/27790437/ventilator-associated-pneumonia-in-an-infant-caused-by-stenotrophomonas-maltophila-a-case-report
#19
Neelima Angaali, Nina Dutta Roy, Sudharshan Raj Chitgupikar, Preeti Subramanian, Jaya Lakshmi Pabbati
Stenotrophomonas maltophila (S.maltophila) is an aerobic Gram-negative bacillus that is a frequent colonizer of fluids used in the hospital setting. The organism is known to cause life threatening infections in immuno-compromised patients especially in those who are neutropenic, on chemotherapy or on broad spectrum antibiotics. We report a case of ventilator associated pneumonia caused by Stenotrophomonas maltophila in a two-month old infant who later developed multi organ dysfunction syndrome. In seriously ill paediatric patients, S...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27774456/potential-of-polycyclic-aromatic-hydrocarbon-degrading-bacterial-isolates-to-contribute-to-soil-fertility
#20
Maryam Bello-Akinosho, Rosina Makofane, Rasheed Adeleke, Mapitsi Thantsha, Michael Pillay, George Johannes Chirima
Restoration of polycyclic aromatic hydrocarbon- (PAH-) polluted sites is presently a major challenge in agroforestry. Consequently, microorganisms with PAH-degradation ability and soil fertility improvement attributes are sought after in order to achieve sustainable remediation of polluted sites. This study isolated PAH-degrading bacteria from enriched cultures of spent automobile engine-oil polluted soil. Isolates' partial 16S rRNA genes were sequenced and taxonomically classified. Isolates were further screened for their soil fertility attributes such as phosphate solubilization, atmospheric nitrogen fixation, and indoleacetic acid (IAA) production...
2016: BioMed Research International
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