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alpha 2 macroglobulin

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https://www.readbyqxmd.com/read/28912679/proteomic-differences-in-blood-plasma-associated-with-antidepressant-treatment-response
#1
Christoph W Turck, Paul C Guest, Giuseppina Maccarrone, Marcus Ising, Stefan Kloiber, Susanne Lucae, Florian Holsboer, Daniel Martins-de-Souza
The current inability of clinical psychiatry to objectively select the most appropriate treatment is a major factor contributing to the severity and clinical burden of major depressive disorder (MDD). Here, we have attempted to identify plasma protein signatures in 39 MDD patients to predict response over a 6-week treatment period with antidepressants. LC-MS/MS analysis showed that differences in the levels of 29 proteins at baseline were found in the group with a favorable treatment outcome. Most of these proteins were components of metabolism or immune response pathways as well as multiple components of the coagulation cascade...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28888879/fast-form-alpha-2-macroglobulin-a-marker-for-protease-activation-in-plasma-exposed-to-artificial-surfaces
#2
Daniel Biltoft, Jørgen B Gram, Anette Larsen, Anna-Marie B Münster, Johannes J Sidelmann, Karsten Skjoedt, Yaseelan Palarasah
OBJECTIVES: Investigation of the blood compatibility requires a number of sensitive assays to quantify the activation of the blood protein cascades and cells induced by biomaterials. A global assay measuring the blood compatibility of biomaterials could be a valuable tool in such regard. In this study, we investigated whether an enzyme-linked immunosorbent assay (ELISA), that specifically measures the electrophoretic "fast form" of α2-macroglobulin (F-α2M), could be a sensitive and global marker for activation of calcium dependent and in-dependent proteases in plasma exposed to biomaterials in vitro...
September 6, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28870784/isotypes-of-autoantibodies-against-differentially-expressed-novel-malondialdehyde-modified-peptide-adducts-in-serum-of-taiwanese-women-with-rheumatoid-arthritis
#3
Chen-Chung Liao, Yu-Sheng Chang, Chao-Wen Cheng, Wei-Ming Chi, Kai-Leun Tsai, Wei-Jung Chen, Ting-Shuan Kung, Chih-Chun Tai, Yi-Fang Lin, Hung-Tse Lin, Yi-Ying Lu, Ching-Yu Lin
This study identified and validated four differentially expressed novel malondialdehyde (MDA)-modified peptide adducts and evaluated autoantibodies against native and MDA-modified peptides among Taiwanese women patients with rheumatoid arthritis (RA), osteoarthritis (OA) and healthy controls (HCs). Ig kappa chain C region(76-99), alpha-1-antitrypsin(284-298), alpha-2-macroglobulin(824-841), and apolipoprotein B-100(4022-4040) exhibiting 2-fold differences in relative modification ratios were identified by concanavalin A (Con A) affinity chromatography, 1D SDS-PAGE, in-gel digestion, nano-LC/MS/MS and nano-LC/MS using pooled serum-derived Con A-captured proteins from 9 RA and 9 age-matched HCs...
September 1, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28760704/determination-of-alpha-2-mrap-gene-polymorphisms-in-nephrolithiasis-patients
#4
Atheer Awad Mehde, Wesen Adel Mehdi, Faridah Yusof, Raha Ahmed Raus, Zaima Azira Zainal Abidin, Hamid Ghazali, Azlina Abd Rahman
BACKGROUND: The intron 5 insertion/deletion polymorphism of Alpha-2-macroglobulin receptor-associated protein gene (Alpha-2-MRAP) has been implicated in numerous diseases. The current study was designed to analyze the association of intron 5 insertion/deletion polymorphism of Alpha-2-MRAP with nephrolithiasis patients. METHODS: PCR was conducted on genomic DNA of patients and control to look for Alpha-2-MRAP insertion/deletion polymorphism. Besides that, serum level of Alpha-2-MRAP, oxidative stress marker myeloperoxidase, Malondialdehyde (MDA), Advanced oxidation protein products (AOPP), and uric acid were determined...
July 29, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28743292/targeted-designed-variants-of-alpha-2-macroglobulin-a2m-attenuate-cartilage-degeneration-in-a-rat-model-of-osteoarthritis-induced-by-anterior-cruciate-ligament-transection
#5
Yang Zhang, Xiaochun Wei, Shawn Browning, Gaetano Scuderi, Lewis S Hanna, Lei Wei
BACKGROUND: The study was performed to evaluate whether targeted alpha-2-macroglobulin (A2M) variants have a similar or enhanced function at wild-type (wt)-A2M to attenuate cartilage degeneration in vivo. METHODS: In and ex-vivo experiment, bovine cartilage explants (BCE) were incubated with TNF-α and IL-1β with or without wt-A2M or A2M variants. Cartilage catabolism was measured in culture supernatant by sulfated glycosaminoglycan (sGAG). In an in-vivo experiment, 2-month-old male Wistar rats (n = 77) were randomly divided into seven groups and treated with different doses of A2M or its variants by intra-articular injection at 24 hours and day 14 after anterior cruciate ligament transection (ACLT), receiving (1) ACLT/PBS; (2) ACLT/wt-A2M (0...
July 25, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28683140/morgagnian-cataract-resulting-from-a-naturally-occurring-nonsense-mutation-elucidates-a-role-of-cpamd8-in-mammalian-lens-development
#6
Anne K Hollmann, Insa Dammann, Wiebke M Wemheuer, Wilhelm E Wemheuer, Almuth Chilla, Andrea Tipold, Walter J Schulz-Schaeffer, Julia Beck, Ekkehard Schütz, Bertram Brenig
To investigate the genetic basis of hereditary lens opacities we analyzed 31 cases of bilateral congenital cataract in Red Holstein Friesian cattle. A genome-wide association study revealed a significant association on bovine chromosome 7 at positions 6,166,179 and 12,429,691. Whole genome re-sequencing of one case and four relatives showed a nonsense mutation (g.5995966C>T) in the PZP-like, alpha-2-macroglobulin domain containing 8 (CPAMD8) gene leading to a premature stop codon (CPAMD8 p.Gln74*) associated with cataract development in cattle...
2017: PloS One
https://www.readbyqxmd.com/read/28681077/direct-analysis-of-site-specific-n-glycopeptides-of-serological-proteins-in-dried-blood-spot-samples
#7
Na Young Choi, Heeyoun Hwang, Eun Sun Ji, Gun Wook Park, Ju Yeon Lee, Hyun Kyoung Lee, Jin Young Kim, Jong Shin Yoo
Dried blood spot (DBS) samples have a number of advantages, especially with respect to ease of collection, transportation, and storage and to reduce biohazard risk. N-glycosylation is a major post-translational modification of proteins in human blood that is related to a variety of biological functions, including metastasis, cell-cell interactions, inflammation, and immunization. Here, we directly analyzed tryptic N-glycopeptides from glycoproteins in DBS samples using liquid chromatography-tandem mass spectrometry (LC-MS/MS) without centrifugation of blood samples, depletion of major proteins, desalting of tryptic peptides, and enrichment of N-glycopeptides...
August 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28627459/peripheral-blood-gene-expression-of-acute-phase-proteins-in-people-with-first-episode-psychosis
#8
Jie Yin Yee, Milawaty Nurjono, Wai Yee Ng, Stephanie Ruth Teo, Tih-Shih Lee, Jimmy Lee
BACKGROUND: There is a growing interest in the association between schizophrenia and the activation of inflammatory system with signs of acute phase (AP) response. Majority of such studies had focused on C-reactive protein (CRP). The aims of the present study were (i) to examine the gene expression profiles of other acute phase proteins (APP), namely haptoglobin (HP), alpha-1 antitrypsin (A1T), and alpha-2 macroglobulin (A2M) in patients with first episode psychosis (FEP) over a period of three months and (ii) to explore the association between APP levels and severity of symptoms...
June 13, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28600207/conformational-behavior-of-alpha-2-macroglobulin-aggregation-and-inhibition-induced-by-tfe
#9
Ahmed Abdur Rehman, Masihuz Zaman, Mohammad Khalid Zia, Haseeb Ahsan, Rizwan Hasan Khan, Fahim Halim Khan
Alpha-2-macroglobulin (α2M), a pan-proteinase inhibitor, inhibits a variety of endogenous and exogenous proteinases and constitutes an important part of body's innate defense system. In the present study, we explored how trifluoroethanol (TFE) may modulate the structure, antiproteinase activity and aggregation of α2M. TFE was sequentially added over a range of 0-20% (v/v) and the effects induced were studied by activity assay, intrinsic fluorescence, ANS fluorescence, circular dichroism, turbidity assay, Rayleigh scattering measurement and ThT fluorescence measurement...
November 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28592917/persistent-unresolved-inflammation-in-the-mecp2-308-female-mutated-mouse-model-of-rett-syndrome
#10
Alessio Cortelazzo, Claudio De Felice, Bianca De Filippis, Laura Ricceri, Giovanni Laviola, Silvia Leoncini, Cinzia Signorini, Monica Pescaglini, Roberto Guerranti, Anna Maria Timperio, Lello Zolla, Lucia Ciccoli, Joussef Hayek
Rett syndrome (RTT) is a rare neurodevelopmental disorder usually caused by mutations in the X-linked gene methyl-CpG-binding protein 2 (MECP2). Several Mecp2 mutant mouse lines have been developed recapitulating part of the clinical features. In particular, Mecp2-308 female heterozygous mice, bearing a truncating mutation, are a validated model of the disease. While recent data suggest a role for inflammation in RTT, little information on the inflammatory status in murine models of the disease is available...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28570120/characterization-of-the-coagulation-profile-in-children-with-liver-disease-and-extrahepatic-portal-vein-obstruction-or-shunt
#11
William Beattie, Maria Magnusson, Winita Hardikar, Paul Monagle, Vera Ignjatovic
INTRODUCTION: Chronic liver disease causes a disruption of procoagulant and anticoagulant factors, resulting in a fragile state, prone to increased rates of both bleeding and thrombosis. Currently, there is limited literature describing the changes observed in pediatric liver disease and extrahepatic portal vein obstruction or shunt. This study aimed to describe the changes that occur in children with chronic liver disease and extrahepatic portal vein obstruction or shunt. MATERIALS AND METHODS: We measured the concentration and activity of key procoagulant and anticoagulant factors in children with liver disease, children with extrahepatic portal vein obstruction or shunt, and healthy children...
June 1, 2017: Pediatric Hematology and Oncology
https://www.readbyqxmd.com/read/28561948/identification-of-fucosylated-fetuin-a-as-a-potential-biomarker-for-cholangiocarcinoma
#12
Lucy Betesh, Mary Ann Comunale, Mengjun Wang, Hongyan Liang, Julie Hafner, Aykan Karabudak, Nasra H Giama, Catherine D Moser, Eiji Miyoshi, Lewis R Roberts, Timothy M Block, Anand Mehta
PURPOSE: Cholangiocarcinoma (CCA) is a malignancy of the bile ducts. The purpose of this discovery study was to identify effective serum markers for surveillance of cholangiocarcinoma. EXPERIMENTAL DESIGN: Using a glycomic method, patients with CCA were determined to have increased levels of alpha-1,3 and alpha-1,6 linked fucosylated glycan. Proteomic analysis of the serum fucosylated proteome identified proteins such as alpha-2-macroglobulin, kininogen, hemopexin, fetuin-A, alpha-1 anti-trypsin, and ceruloplasmin as being hyperfucosylated in HCC...
September 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28558995/cellular-uptake-of-coagulation-factor-viii-elusive-role-of-the-membrane-binding-spikes-in-the-c1-domain
#13
Lydia Castro-Núñez, Johanna M Koornneef, Mariska G Rondaij, Esther Bloem, Carmen van der Zwaan, Koen Mertens, Alexander B Meijer, Henriet Meems
Low density lipoprotein receptor-related protein 1 (LRP1) is involved in the catabolism of many ligands, including factor VIII (FVIII) and alpha-2-macroglobulin (α2M). Transfer of FVIII to LRP1 is currently believed to be preceded by pre-concentration on the cell surface, by interacting with a so far unidentified component. In the present study, we used confocal microscopy and flow cytometry to compare endocytosis of FVIII and α2M using U87MG cells. The results show that α2M is rapidly internalized and does not compete for LRP1 mediated internalization of FVIII...
May 27, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28513463/alzheimer-s-disease-against-peptides-products-of-enzymatic-cleavage-app-protein-biological-pathobiological-and-physico-chemical-properties-of-fibrillating-peptides
#14
Małgorzata Marszałek
Various peptides products of enzymatic cleavage of key for Alzheimer's disease Amyloid Precursor Protein (APP) are well known, but still are matter of scientific debate. The Aβ type products are especially challenging for experimental and medical research. This paper outlines several, still poorly known, biological and medical processes such as peptides biology, i.e., formation, biodistribution, translocation, transport and finally removal from brain compartments and body fluids like Intracellular Fluid (ICF), Cerebrospinal Fluid (CSF), Interstitial Fluid (ISF), blood serum or urine...
May 17, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/28502560/a-microscale-protocol-for-the-isolation-of-transferrin-directly-from-serum
#15
Ana Penezić, Goran Miljuš, Bojana Milutinović, Olgica Nedić
A microscale procedure for the isolation of transferrin directly from human serum (hTf) is described in this study. The protocol is based on three precipitation steps without application of chromatography. It lasts 90min with the initial sample volume of 250μL. The yield of the isolated hTf is 58%, which is considerable in biochemical terms. The purity of the isolated hTf is 97%, as assessed by three methods: electrophoresis followed by protein staining, immunoblotting and HPLC. Immunoblotting with antibodies against other major serum proteins indicated that isolated hTf does not contain albumin, immunoglobulin G or alpha-2-macroglobulin...
May 11, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28469399/a-novel-biomarker-panel-to-identify-steroid-resistance-in-childhood-idiopathic-nephrotic-syndrome
#16
Michael R Bennett, LaTawnya Pleasant, Christopher Haffner, Qing Ma, Wendy D Haffey, Jun Ying, Michael Wagner, Kenneth D Greis, Prasad Devarajan
Idiopathic nephrotic syndrome (NS) is the most common glomerular disorder of childhood. Response to initial treatment with corticosteroids is an indicator of prognosis, as resistant patients often present more progressive disease. In this cross-sectional pilot study, we set out to discover a panel of noninvasive biomarkers that could distinguish steroid-resistant nephrotic syndrome (SRNS) from steroid-sensitive nephrotic syndrome (SSNS). Information gleaned from such a panel could yield more individualized treatment plans and prevent unnecessary steroid exposure in patients unlikely to respond...
2017: Biomarker Insights
https://www.readbyqxmd.com/read/28454818/plasma-immune-protein-analysis-in-the-orange-spotted-grouper-epinephelus-coioides-evidence-for-altered-expressions-of-immune-factors-associated-with-a-choline-supplemented-diet
#17
Ya-Li Shiu, Kuo-Hsun Chiu, Truong-Giang Huynh, Ping-Chung Liu, Chun-Hung Liu
This study aimed to unravel the regulatory roles of choline in activating immune responses and disease resistance of the orange-spotted grouper Epinephelus coioides. Fish were fed a choline-supplemented diet at 1 g kg(-1) of feed for 30 days. Fish fed a fish meal basal diet without choline-supplement served as controls. At the end of the feeding trial, fish were challenged with Vibrio alginolyticus. Meanwhile, plasma proteomics of fish in each group were also evaluated by two-dimensional gel electrophoresis (2-DE), and differentially expressed proteins were identified by tandem mass spectrophotometry (MS/MS), then a Western blot analysis or real-time polymerase chain reaction was used to confirm differential expressions of immune-enhancing proteins...
April 25, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28453489/changes-of-cerebrospinal-fluid-peptides-due-to-tauopathy
#18
Petra Majerova, Peter Barath, Alena Michalicova, Stanislav Katina, Michal Novak, Andrej Kovac
Alzheimer's disease (AD) and progressive supranuclear palsy are two common neurodegenerative tauopathies, and the most common cause of progressive brain dementia in elderly affecting more than 35 million people. The tauopathies are characterized by abnormal deposition of microtubule associated protein tau into intracellular neurofibrillary tangles composed mainly of the hyperphosphorylated form of the protein. The diagnosis of tauopathies is based on the presence of clinical features and pathological changes...
2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28452727/-the-influence-of-lifestyle-on-biomarkers-of-fibrosis-in-patients-with-stable-coronary-heart-disease-on-the-background-of-non-alcoholic-fatty-liver-disease
#19
N Virstyuk, I Vakalyuk
Purpose - to assess the impact of lifestyle on non-alcoholic fatty liver disease (NAFLD) activity by influencing the fibrotic processes and hepatic steatosis in patients with stable coronary heart disease (CHD). 105 patients with stable CHD, combined with NAFLD were observed: Group I - control group; Group II - basic group. In each group, patients with non-alcoholic steatosis (subgroup A) and non-alcoholic steatohepatitis (subgroup B) were isolated. General clinical examination, electrocardiography, coronary angiography, echocardiography, liver functional state, serum lipid profile, fasting glucose, markers of fibrosis were conducted to all patients...
February 2017: Georgian Medical News
https://www.readbyqxmd.com/read/28449136/-alpha-2-macroglobulin-serum-level-in-patients-with-alpha-1-antitrypsin-deficiency
#20
V Kotke, S Wiedmann, C Nell, C Vogelmeier, R Bals, T Greulich, A Klemmer
Background and objectives Alpha-2 Macroglobulin (A2M) is a plasma protein with proteolytic effects on many proteases. In patients with an inborn alpha-1 antitrypsin deficiency (AATD) the homeostasis between proteases and antiproteases is disturbed. The aim of this study was to compare the levels of AAT and A2 M in patients and controls. We hypothesized that in patients with AATD A2 M levels are elevated. Methods Patients with AATD (polymorphism Pi*ZZ, Pi*SZ, Pi*MZ and rare gene variants) as well as healthy volunteers (Pi*MM) were tested for A2 M and AAT levels...
September 2017: Pneumologie
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