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alpha 2 macroglobulin

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https://www.readbyqxmd.com/read/29778882/insight-into-the-interactions-of-proteinase-inhibitor-alpha-2-macroglobulin-with-hypochlorite-thermal-analysis-and-biophysical-approach
#1
Tooba Siddiqui, Mohammad Khalid Zia, Syed Saqib Ali, Haseeb Ahsan, Fahim Halim Khan
Hypochlorous acid, an active bleaching agent is one of the major oxidants produced by neutrophils under physiological conditions. It is a potent reactive oxygen species (ROS) which causes oxidation of biomolecules. Treatment of proteins with hypochlorite results in direct oxidative damage to proteins. Alpha-2-macroglobulin is a major proteinase inhibitor and it can inhibit proteinase of any kind regardless of specificity and catalytic mechanism. The proteinase-antiproteinase balance plays an important role in mediating inflammation associated tissue destruction...
May 17, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29751037/interaction-of-anti-cancer-drug-cisplatin-with-major-proteinase-inhibitor-alpha-2-macroglobulin-biophysical-and-thermodynamic-analysis
#2
Mohammad Khalid Zia, Tooba Siddiqui, Syed Saqib Ali, Haseeb Ahsan, FahimHalim Khan
Alpha-2-macroglobulin is a multifunctional, highly abundant, plasma protein which reacts with a wide variety of molecules and drugs such as cisplatin. Cisplatin is commonly used anticancer drug widely used for treatment of testicular, bladder, ovarian, head and neck, lung and cervical cancers. This study is designed to examine the interaction of cisplatin with human alpha-2-macroglobulin through various biophysical techniques and drug binding through molecular modeling. Cisplatin alters the function of alpha-2-macroglobulin and the thiolesters are most likely the reactive sites for cisplatin...
May 8, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29749538/trim58-cg26157385-methylation-is-associated-with-eight-prognostic-genes-in-lung-squamous-cell-carcinoma
#3
Weimin Zhang, Qichen Cui, Weifeng Qu, Xiaoyun Ding, Donglin Jiang, Hongcheng Liu
The present study aimed to analyze the differentially expressed genes related to the tripartite motif containing 58 (TRIM58)/cg26157385 methylation sites, and consequently to provide theoretical basis for elucidating the influence of TRIM58/cg26157385 methylation on lung cancer prognosis. Methylation‑sequencing information, mRNA expression profiling data and clinical data were downloaded from cBioPortal database to screen out candidate genes related to the methylation of TRIM58/cg26157385 in squamous cell lung carcinoma...
May 8, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29709984/the-protobothrops-flavoviridis-hemorrhagic-metalloproteinase-hr2-is-inhibited-by-human-alpha-2-macroglobulin
#4
Nobuya Morine, Seiko Matsuda, Yumiko Izumi, Akiko Oshiro, Jun Kudaka
Habu (Protobothrops flavoviridis) venom toxoid vaccination was performed in individuals living in Amami-oshima from 1965 to 2002, the effectiveness of which was investigated in 1991. The results raised the possibility that normal human serum inherently contains an inhibitor of the hemorrhagic metalloproteinase HR2, considered to be one of the major components of habu venom. In this study, we investigated the interaction between the hemorrhagic metalloproteinases HR1 and HR2 from habu venom and human α2-macroglobulin...
April 27, 2018: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29692335/study-of-the-plasma-proteome-of-atlantic-cod-gadus-morhua-changes-due-to-crude-oil-exposure
#5
Karianne S Enerstvedt, Magne O Sydnes, Daniela M Pampanin
Oil contamination is an environmental issue of great concern and the necessity for background studies and monitoring programs to continuously evaluate the levels of oil pollution is required. In this study, Atlantic cod (Gadus morhua) were exposed to dispersed crude oil for 1 and 4 weeks to simulate environmental contamination. Fractionated plasma samples were then analysed by tandem mass spectrometry. In total, 717 proteins were identified and 10 new protein biomarker candidates were found. The significant proteome changes were related to the immune response by alterations in the levels of specific immunoglobulins, alpha-2-macroglobulin and galectin-3-binding proteins...
April 17, 2018: Marine Environmental Research
https://www.readbyqxmd.com/read/29665821/plasma-proteomic-analysis-reveals-altered-protein-abundances-in-cardiovascular-disease
#6
Vasiliki Lygirou, Agnieszka Latosinska, Manousos Makridakis, William Mullen, Christian Delles, Joost P Schanstra, Jerome Zoidakis, Burkert Pieske, Harald Mischak, Antonia Vlahou
BACKGROUND: Cardiovascular disease (CVD) describes the pathological conditions of the heart and blood vessels. Despite the large number of studies on CVD and its etiology, its key modulators remain largely unknown. To this end, we performed a comprehensive proteomic analysis of blood plasma, with the scope to identify disease-associated changes after placing them in the context of existing knowledge, and generate a well characterized dataset for further use in CVD multi-omics integrative analysis...
April 17, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29659051/psa-alpha-2-macroglobulin-complex-is-enzymatically-active-in-the-serum-of-patients-with-advanced-prostate-cancer-and-can-degrade-circulating-peptide-hormones
#7
Maya B Kostova, William Nathaniel Brennen, David Lopez, Lizamma Anthony, Hao Wang, Elizabeth Platz, Samuel R Denmeade
BACKGROUND: Prostate cancer cells produce high levels of the serine protease Prostate-Specific Antigen (PSA). PSA is enzymatically active in the tumor microenvironment but is presumed to be enzymatically inactive in the blood due to complex formation with serum protease inhibitors α-1-antichymotrypsin and α-2-macroglobulin (A2M). PSA-A2M complexes cannot be measured by standard ELISA assays and are also rapidly cleared from the circulation. Thus the exact magnitude of PSA production by prostate cancer cells is not easily measured...
April 16, 2018: Prostate
https://www.readbyqxmd.com/read/29625245/itraq-based-quantitative-proteomic-analysis-of-procambarus-clakii-hemocytes-during-spiroplasma-eriocheiris-infection
#8
Yinbin Xu, Wenjing Hao, Tao Xiang, Haifeng Zhou, Wen Wang, Qingguo Meng, Zhengfeng Ding
As a new-found aquaculture pathogen, Spiroplasma eriocheiris, has resulted in inconceivable economic losses in aquaculture. In the infection of S. eriocheiris, the Procambarus clakii hemocytes have indicated to be major target cells. What was designed to examine in our study is the hemocytes' immune response at the protein levels. Before the pathogen was injected and after 192 h of post-injection, the differential proteomes of the crayfish hemocytes were analyzed immediately by isobaric tags for relative and absolute quantization (iTRAQ) labeling, followed by liquid chromatogramphytandem mass spectrometry (LC-MS/MS)...
April 3, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29564590/proteomic-analysis-of-the-antidepressant-effects-of-shen-zhi-ling-in-depressed-patients-identification-of-proteins-associated-with-platelet-activation-and-lipid-metabolism
#9
Chao Chen, Yuan Hu, Xian-Zhe Dong, Xiao-Jiang Zhou, Li-Hua Mu, Ping Liu
Shen-Zhi-Ling (SZL) is a Chinese medicine formulated from a Kai-Xin-San decoction that is commonly used to treat depression caused by dual deficiencies in the heart and spleen. However, the underlying mechanisms remain unclear. We investigated biological changes in depression patients (DPs) exhibiting antidepressant responses to SZL treatment using proteomic techniques. We performed label-free quantitative proteomic analysis and liquid chromatography-tandem mass spectrometry to discover and examine altered proteins involved in depression and antidepressant treatment...
March 21, 2018: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/29544770/acclimation-to-cold-and-warm-temperatures-is-associated-with-differential-expression-of-male-carp-blood-proteins-involved-in-acute-phase-and-stress-responses-and-lipid-metabolism
#10
Mariola A Dietrich, Piotr Hliwa, Mikołaj Adamek, Dieter Steinhagen, Halina Karol, Andrzej Ciereszko
The environmental temperature affects plasma biochemical indicators, antioxidant status and hematological and immunological parameters in fish. So far, only single blood proteins have been identified in response to temperature changes. The aim of this study was to compare the proteome of carp blood plasma from males acclimated to warm (30 °C) and cold (10 °C) temperatures by two-dimensional differential gel electrophoresis followed by MALDI-TOF/TOF mass spectrometry. A total of 47 spots were found to be differentially regulated by temperature (>1...
March 12, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29518990/biosynthesis-of-rishirilide-b
#11
Philipp Schwarzer, Julia Wunsch-Palasis, Andreas Bechthold, Thomas Paululat
Rishirilide B was isolated from Streptomyces rishiriensis and Streptomyces bottropensis on the basis of its inhibitory activity towards alpha-2-macroglobulin. The biosynthesis of rishirilide B was investigated by feeding experiments with different 13 C labelled precursors using the heterologous host Streptomyces albus J1074::cos4 containing a cosmid encoding of the gene cluster responsible for rishirilide B production. NMR spectroscopic analysis of labelled compounds demonstrate that the tricyclic backbone of rishirilide B is a polyketide synthesized from nine acetate units...
March 7, 2018: Antibiotics
https://www.readbyqxmd.com/read/29486203/a-method-for-the-extraction-of-the-endogenous-tryptic-peptides-peptidome-from-human-edta-plasma
#12
Jaimie Dufresne, Angelique Florentinus-Mefailoski, Pete Bowden, John G Marshall
The proteins identified from endogenous peptides agreed between serum versus plasma, and tryptic versus non-tryptic peptides, when collected by C18 alone and analyzed by liquid chromatography electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS) including amyloids, apolipoproteins, haptoglobin, complements, fibrinogens, hemopexin, antitrypsin and alpha 2 macroglobulin. Precipitation of polypeptides from plasma in 9 vol of 100% organic solvent followed by stepwise extraction of the insoluble pellet with an increasing fraction of water identified thousands of proteins...
May 15, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29475030/screening-for-protein-adducts-of-naphthalene-and-chrysene-in-plasma-of-exposed-atlantic-cod-gadus-morhua
#13
Karianne S Enerstvedt, Magne O Sydnes, Eivind Larssen, Daniela M Pampanin
Polycyclic aromatic hydrocarbons (PAHs) are well known contaminants, ubiquitously present in the habitat and spawning areas for Atlantic cod (Gadus morhua). The Atlantic cod is a key species and a globally important food source, thus continuous monitoring of PAHs is considered highly valuable to ensure ecosystem sustainability and human food safety. PAH adducts to plasma proteins are applied as sensitive biomarkers of PAH exposure in humans and other species, thus the presence of PAH protein adducts in Atlantic cod plasma was investigated to identify PAH protein adduct biomarker candidates of exposure to PAHs...
June 2018: Chemosphere
https://www.readbyqxmd.com/read/29407617/marine-actinomycetes-as-bioremediators-in-penaeus-monodon-rearing-system
#14
Divya T Babu, K Archana, Bhavya Kachiprath, Solly Solomon, G Jayanath, I S Bright Singh, Rosamma Philip
Actinomycetes (277 Nos) isolated from marine environment and shrimp culture pond sediments were tested for hydrolytic enzyme production and biogranulation property. Potential isolates were screened for their efficacy in bioremediation of shrimp culture system. Based on the BOD reduction efficiency and water quality parameters, five actinomycete isolates viz., Streptomyces coelicoflavus (A6), Streptomyces diastaticus (A44), Nocardiopsis alba (A55), Streptomyces parvus (A56) and Streptomyces champavatii (R32) were subjected for tertiary screening in Penaeus monodon larval rearing system and the animals were challenged with white spot syndrome virus (WSSV)...
April 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29342131/survey-of-plasma-proteins-in-children-with-progeria-pre-therapy-and-on-therapy-with-lonafarnib
#15
Leslie B Gordon, Susan E Campbell, Joseph M Massaro, Ralph B D'Agostino, Monica E Kleinman, Mark W Kieran, Marsha A Moses
BACKGROUND: Hutchinson-Gilford progeria syndrome (HGPS) is an ultra-rare, fatal, segmental premature aging syndrome caused by the aberrant lamin A protein, progerin. The protein farnesyltransferase inhibitor, lonafarnib, ameliorates some aspects of cardiovascular and bone disease. STUDY DESIGN: We performed a prospective longitudinal survey of plasma proteins in 24 children with HGPS (an estimated 10% of the world's population at the time) at baseline and on lonafarnib therapy, compared to age and gender-matched controls using a multi-analyte, microsphere-based immune-fluorescent assay...
January 17, 2018: Pediatric Research
https://www.readbyqxmd.com/read/29323132/assembly-of-an-atypical-%C3%AE-macroglobulin-complex-from-pseudomonas-aeruginosa
#16
Samira Zouhir, Mylène Robert-Genthon, Daniel Maragno Trindade, Viviana Job, Marko Nedeljković, Cécile Breyton, Christine Ebel, Ina Attrée, Andréa Dessen
Alpha-2-macroglobulins (A2Ms) are large spectrum protease inhibitors that are major components of the eukaryotic immune system. Pathogenic and colonizing bacteria, such as the opportunistic pathogen Pseudomonas aeruginosa, also carry structural homologs of eukaryotic A2Ms. Two types of bacterial A2Ms have been identified: Type I, much like the eukaryotic form, displays a conserved thioester that is essential for protease targeting, and Type II, which lacks the thioester and to date has been poorly studied despite its ubiquitous presence in Gram-negatives...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29302075/foxo1-a2m-and-tgf-%C3%AE-1-three-novel-genes-predicting-depression-in-gene-x-environment-interactions-are-identified-using-cross-species-and-cross-tissues-transcriptomic-and-mirnomic-analyses
#17
Annamaria Cattaneo, Nadia Cattane, Chiara Malpighi, Darina Czamara, Anna Suarez, Nicole Mariani, Eero Kajantie, Alessia Luoni, Johan G Eriksson, Jari Lahti, Valeria Mondelli, Paola Dazzan, Katri Räikkönen, Elisabeth B Binder, Marco A Riva, Carmine M Pariante
To date, gene-environment (GxE) interaction studies in depression have been limited to hypothesis-based candidate genes, since genome-wide (GWAS)-based GxE interaction studies would require enormous datasets with genetics, environmental, and clinical variables. We used a novel, cross-species and cross-tissues "omics" approach to identify genes predicting depression in response to stress in GxE interactions. We integrated the transcriptome and miRNome profiles from the hippocampus of adult rats exposed to prenatal stress (PNS) with transcriptome data obtained from blood mRNA of adult humans exposed to early life trauma, using a stringent statistical analyses pathway...
January 4, 2018: Molecular Psychiatry
https://www.readbyqxmd.com/read/29296203/the-pathogenesis-of-diclofenac-induced-immunoallergic-hepatitis-in-a-canine-model-of-liver-injury
#18
Saravanakumar Selvaraj, Jung-Hwa Oh, Reinhard Spanel, Florian Länger, Hyoung-Yun Han, Eun-Hee Lee, Seokjoo Yoon, Jürgen Borlak
Hypersensitivity to non-steroidal anti-inflammatory drugs is a common adverse drug reaction and may result in serious inflammatory reactions of the liver. To investigate mechanism of immunoallergic hepatitis beagle dogs were given 1 or 3 mg/kg/day (HD) oral diclofenac for 28 days. HD diclofenac treatment caused liver function test abnormalities, reduced haematocrit and haemoglobin but induced reticulocyte, WBC, platelet, neutrophil and eosinophil counts. Histopathology evidenced hepatic steatosis and glycogen depletion, apoptosis, acute lobular hepatitis, granulomas and mastocytosis...
December 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/29287080/serum-apolipoprotein-a1-and-haptoglobin-in-patients-with-suspected-drug-induced-liver-injury-dili-as-biomarkers-of-recovery
#19
Valentina Peta, Chantal Tse, Hugo Perazzo, Mona Munteanu, Yen Ngo, An Ngo, Nittia Ramanujam, Lea Verglas, Maxime Mallet, Vlad Ratziu, Dominique Thabut, Marika Rudler, Vincent Thibault, Ina Schuppe-Koistinen, Dominique Bonnefont-Rousselot, Bernard Hainque, Françoise Imbert-Bismut, Michael Merz, Gerd Kullak-Ublick, Raul Andrade, Florian van Boemmel, Eckart Schott, Thierry Poynard
BACKGROUND: There is a clear need for better biomarkers of drug-induced-liver-injury (DILI). AIMS: We aimed to evaluate the possible prognostic value of ActiTest and FibroTest proteins apoliprotein-A1, haptoglobin and alpha-2-macroglobulin, in patients with DILI. METHODS: We analyzed cases and controls included in the IMI-SAFE-T-DILI European project, from which serum samples had been stored in a dedicated biobank. The analyses of ActiTest and FibroTest had been prospectively scheduled...
2017: PloS One
https://www.readbyqxmd.com/read/29284148/itraq-based-comparative-proteome-analysis-for-molecular-mechanism-of-defense-against-acute-ammonia-toxicity-in-pacific-white-shrimp-litopenaeus-vannamei
#20
Xia Lu, Sheng Luan, Ping Dai, Xianhong Meng, Baoxiang Cao, Kun Luo, Jie Kong
In the practical farming of Litopenaeus vannamei, the intensive culture system and environmental pollution usually results in a high concentration of ammonia, which brings large detrimental effects to shrimp, such as increasing the susceptibility to pathogens and even causing high mortality. We have revealed that the survival time under acute ammonia stress varied substantially among different families and obtained ammonia-tolerant (LV_T) and ammonia-sensitive (LV_S) families. In order to understand the molecular mechanism of defense against ammonia toxicity in shrimp, we performed iTRAQ LC-MS/MS proteomic analysis between LV_T and LV_S groups of L...
March 2018: Fish & Shellfish Immunology
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