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https://www.readbyqxmd.com/read/28239846/inhibition-of-6-phosphofructo-2-kinase-suppresses-fibroblast-like-synoviocytes-mediated-synovial-inflammation-and-joint-destruction-in-rheumatoid-arthritis
#1
Yaoyao Zou, Shan Zeng, Mingcheng Huang, Qian Qiu, Youjun Xiao, Maohua Shi, Zhongping Zhan, Liuqin Liang, Xiuyan Yang, Hanshi Xu
BACKGROUND AND PURPOSE: Abnormal glycolytic metabolism contributes to joint inflammation in rheumatoid arthritis (RA). The aims of this study were to investigate the role of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3), a bifunctional enzyme that controls the glycolytic rate, in regulating fibroblast-like synoviocyte (FLS)-mediated synovial inflammation and invasiveness in RA. EXPERIMENTAL APPROACH: A specific PFKFB3 inhibitor, PFK15, and siRNA were used to evaluate the role of PFKFB3...
February 27, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28239539/metabolic-syndrome-s-risk-factors-and-its-association-with-nutritional-status-in-schoolchildren
#2
Fabiana Costa Teixeira, Flavia Erika Felix Pereira, Avany Fernandes Pereira, Beatriz Gonçalves Ribeiro
The metabolic risk factors (RF) to the diagnosis of metabolic syndrome (MetS) have been evidenced at early ages, including children. The aim of the present study was to identify the prevalence of RF to the diagnosis of MetS and its association with nutritional status of schoolchildren from 6 to 10 years old. A cross-sectional study was carried out in 505 students of municipal schools in Macae, Brazil, conducted from 2013 to 2014. The RF evaluated were: blood pressure (mm Hg), triglycerides (mmol/L), HDL-cholesterol (mmol/L) fasting glucose (mmol/L) and waist circumference (cm)...
June 2017: Preventive Medicine Reports
https://www.readbyqxmd.com/read/28239482/aggressive-clinical-approach-to-obesity-improves-metabolic-and-clinical-outcomes-and-can-prevent-bariatric-surgery-a-single-center-experience
#3
Flavio A Cadegiani, Gustavo C Diniz, Gabriella Alves
BACKGROUND: The number of bariatric procedures has exponentially increased in the past decade, as a result of the lack of successful clinical weight-loss interventions. The main reasons for the failure of clinical obesity management are: (1) anti-obesity medications are administered as monotherapies (or pre-combined drugs); (2) lack of combination between pharmacotherapy and non-pharmacological modalities; (3) short duration of pharmacotherapy for obesity; (4) lack of weight-loss maintenance strategies; (5) misunderstanding of the complex pathophysiology of obesity; and (6) underprescription of anti-obesity medications...
2017: BMC Obesity
https://www.readbyqxmd.com/read/28239412/genetic-engineering-of-arabidopsis-to-overproduce-disinapoyl-esters-potential-lignin-modification-molecules
#4
Shinyoung Lee, Huaping Mo, Jeong Im Kim, Clint Chapple
BACKGROUND: Monolignol-like molecules can be integrated into lignin along with conventional monolignol units, and it has been shown that the incorporation of non-canonical subunits can be used to generate hydrolysable lignin by introduction of ester linkages into the polymer and that this type of lignin is more easily removable. Disinapoyl esters (DSEs), which to some degree resemble the monolignol sinapyl alcohol, may be promising lignin modifying units for this purpose. As a first step toward determining whether this goal is achievable, we manipulated metabolic flux in Arabidopsis to increase the amounts of DSEs by overexpressing sinapoylglucose:sinapoylglucose sinapoyltransferase (SST) which produces two main DSEs, 1,2-disinapoylglucose, and another compound we identify in this report as 3,4-disinapoyl-fructopyranose...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28239403/lipid-overload-during-gestation-and-lactation-can-independently-alter-lipid-homeostasis-in-offspring-and-promote-metabolic-impairment-after-new-challenge-to-high-fat-diet
#5
Laís Angélica de Paula Simino, Thaís de Fante, Marina Figueiredo Fontana, Fernanda Oliveira Borges, Márcio Alberto Torsoni, Marciane Milanski, Lício Augusto Velloso, Adriana Souza Torsoni
BACKGROUND: Nutritional status in early life is critically involved in the metabolic phenotype of offspring. However the changes triggered by maternal consumption of high-fat diet (HFD) in pre- or postnatal period should be better understood. Here we evaluated whether maternal HFD consumption during gestation and lactation could differently affect liver miR-122 and miR-370 expression leading to metabolic damages observed in offspring. Moreover, we investigate whether early overnutrition program offspring to more harmful response to HFD in later life...
2017: Nutrition & Metabolism
https://www.readbyqxmd.com/read/28239181/high-dietary-magnesium-intake-is-significantly-and-independently-associated-with-higher-insulin-sensitivity-in-a-mexican-mestizo-population-a-brief-cross-sectional-report
#6
Carlos Moctezuma-Velázquez, Miguel Ángel Gómez-Sámano, María Belén Cajas-Sánchez, Diana Lorena Reyes-Molina, Mariana Galindo-Guzmán, Clara-Elena Meza-Arana, Daniel Cuevas-Ramos, Francisco Javier Gómez-Pérez, Alfonso Gulias-Herrero
BACKGROUND: Magnesium acts as a cofactor in many intracellular reactions including phosphorylation of the insulin receptor; therefore, its imbalance can potentially cause insulin resistance. Low serum magnesium concentration has been associated with the development of metabolic syndrome and type 2 diabetes mellitus. OBJECTIVE: To study the association between the daily dietary magnesium intake and insulin resistance estimated by the homeostatic model assessment of insulin resistance and homeostatic model assessment 2, as well as insulin sensitivity estimated by the Matsuda index...
January 2017: Revista de Investigación Clínica; Organo del Hospital de Enfermedades de la Nutrición
https://www.readbyqxmd.com/read/28239025/the-role-of-single-nucleotide-variants-of-the-energy-metabolism-linked-genes-sirt3-ppargc1a-and-apoe-in-amyotrophic-lateral-sclerosis-risk
#7
Diego Albani, Elisabetta Pupillo, Elisa Bianchi, Armando Chierchia, Rosalba Martines, Gianluigi Forloni, Ettore Beghi
Amyotrophic lateral sclerosis (ALS) is a multifactorial disease, possibly with contributions from genetics and lifestyle. We examined variants in genes relevant to energy metabolism and physical activity in a case-control association study, with the aim of assessing genetics and physical activity as contributors to ALS risk. A well-characterized sample of Italian ALS patients (101) and controls (101) from the EURALS Consortium underwent a questionnaire interview on demographic, physical and other lifestyle habits, and venipuncture for DNA extraction...
February 24, 2017: Genes & Genetic Systems
https://www.readbyqxmd.com/read/28238901/evaluation-of-14-c-and-13-c-sucrose-as-blood-brain-barrier-permeability-markers
#8
Mohammad K Miah, Ekram A Chowdhury, Ulrich Bickel, Reza Mehvar
Non-specific quantitation of [(14)C]sucrose in blood and brain has been routinely used as a quantitative measure of the in vivo blood-brain barrier (BBB) integrity. However, the reported apparent brain uptake clearance (Kin) of the marker varies widely (∼100 fold). We investigated the accuracy of the use of the marker in comparison with a stable isotope of sucrose ([(13)C]sucrose) measured by a specific LC-MS/MS method. Rats received single doses of each marker, and the Kin values were determined. Surprisingly, the Kin value of [(13)C]sucrose was 6-7 fold lower than that of [(14)C]sucrose...
February 23, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28238814/neural-basis-of-cognitive-assessment-in-alzheimer-disease-amnestic-mild-cognitive-impairment-and-subjective-memory-complaints
#9
Jordi A Matías-Guiu, María Nieves Cabrera-Martín, María Valles-Salgado, Alicia Pérez-Pérez, Teresa Rognoni, Teresa Moreno-Ramos, José Luis Carreras, Jorge Matías-Guiu
INTRODUCTION: Interpreting cognitive tests is often challenging. The same test frequently examines multiple cognitive functions, and the functional and anatomical basis underlying test performance is unknown in many cases. This study analyses the correlation of different neuropsychological test results with brain metabolism in a series of patients evaluated for suspected Alzheimer disease. METHODS: 20 healthy controls and 80 patients consulting for memory loss were included, in which cognitive study and (18)F-fluorodeoxyglucose PET were performed...
February 3, 2017: American Journal of Geriatric Psychiatry
https://www.readbyqxmd.com/read/28238759/in-vitro-metabolic-engineering-for-the-production-of-%C3%AE-ketoglutarate
#10
Barbara Beer, André Pick, Volker Sieber
α-Ketoglutarate (aKG) represents a central intermediate of cell metabolism. It is used for medical treatments and as a chemical building block. Enzymatic cascade reactions have the potential to sustainably synthesize this natural product. Here we report a systems biocatalysis approach for an in vitro reaction set-up to produce aKG from glucuronate using the oxidative pathway of uronic acids. Because of two dehydrations, a decarboxylation, and reaction conditions favoring oxidation, the pathway is driven thermodynamically towards complete product formation...
February 22, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28238722/hormetic-modulation-of-hepatic-insulin-sensitivity-by-advanced-glycation-end-products
#11
Nelly T Fabre, Karina Thieme, Karolline S Silva, Sérgio Catanozi, Ana Mercedes Cavaleiro, Danilo A C Pinto, Maristela M Okamoto, Mychel Raony P T Morais, Bárbara Falquetto, Telma M Zorn, Ubiratan F Machado, Marisa Passarelli, Maria Lúcia Correa-Giannella
Because of the paucity of information regarding metabolic effects of advanced glycation end products (AGEs) on liver, we evaluated effects of AGEs chronic administration in (1) insulin sensitivity; (2) hepatic expression of genes involved in AGEs, glucose and fat metabolism, oxidative stress and inflammation and; (3) hepatic morphology and glycogen content. Rats received intraperitoneally albumin modified (AlbAGE) or not by advanced glycation for 12 weeks. AlbAGE induced whole-body insulin resistance concomitantly with increased hepatic insulin sensitivity, evidenced by activation of AKT, inactivation of GSK3, increased hepatic glycogen content, and decreased expression of gluconeogenesis genes...
February 23, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28238672/thermodynamics-of-interactions-between-mammalian-cytochromes-p450-and-b5
#12
Evgeny Yablokov, Anna Florinskaya, Alexei Medvedev, Gennady Sergeev, Natallia Strushkevich, Alexander Luschik, Tatsiana Shkel, Irina Haidukevich, Andrei Gilep, Sergey Usanov, Alexis Ivanov
Cytochromes P450 (CYPs) play an important role in the metabolism of xenobiotics and various endogenous substrates. Being a crucial component of the microsomal monooxygenase system, CYPs are involved in numerous protein-protein interactions. However, mechanisms underlying molecular interactions between components of the monooxygenase system still need better characterization. In this study thermodynamic parameters of paired interactions between mammalian CYPs and cytochromes b5 (CYB5) have been evaluated using a Surface Plasmon Resonance (SPR) based biosensor Biacore 3000...
February 23, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28238629/apolipoprotein-a1-rs5070-a-g-polymorphism-with-stroke-subtypes-in-taiwan
#13
Li-Chi Hsu, Tsong-Hai Lee
BACKGROUND: Apolipoprotein A1 (ApoA1) is a structural protein of high-density lipoprotein cholesterol; ApoA1 is involved in lipid and cholesterol metabolism. This study evaluated the association of ApoA1 polymorphism (rs5070) with different stroke subtypes in Taiwanese individuals. METHODS: A total of 2139 cases, including 614 controls and 708 large artery atherosclerosis (LAA), 377 small-vessel occlusion, and 440 hypertensive intracranial hemorrhage cases, were enrolled in this study...
February 23, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/28238530/a-membrane-associated-adenylate-cyclase-modulates-lactate-dehydrogenase-and-creatine-kinase-activities-required-for-bull-sperm-capacitation-induced-by-hyaluronic-acid
#14
Silvina Fernández, Mariana Córdoba
Hyaluronic acid, as well as heparin, is a glycosaminoglycan present in the female genital tract of cattle. The aim of this study was to evaluate oxidative metabolism and intracellular signals mediated by a membrane-associated adenylate cyclase (mAC), in sperm capacitation with hyaluronic acid and heparin, in cryopreserved bull sperm. The mAC inhibitor, 2',5'-dideoxyadenosine, was used in the present study. Lactate dehydrogenase (LDH) and creatine kinase (CK) activities and lactate concentration were determined spectrophotometrically in the incubation medium...
February 20, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28238496/prevalence-of-polycystic-ovary-syndrome-in-chinese-obese-women-of-reproductive-age-with-or-without-metabolic-syndrome
#15
Peiwen Liang, Liuqing Xi, Juan Shi, Wanyu Li, Shaoqian Zhao, Yuying Deng, Rui Wang, Yingkai Sun, Bin Gu, Lihui Yuan, Yifei Zhang, Weiqiong Gu, Weiqing Wang, Jie Hong
OBJECTIVE: To compare the prevalence of polycystic ovary syndrome (PCOS) and related clinical characteristics between metabolically unhealthy obese (MUO) and metabolically healthy obese (MHO) women of reproductive age. DESIGN: Cross-sectional clinical study. SETTING: Tertiary hospital. PATIENT(S): We studied 299 MUO and 122 MHO Chinese women matched on body mass index. Metabolically healthy obese was defined as obesity with no more than one metabolic abnormality...
February 23, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28238323/design-of-strategies-to-study-the-metabolic-profile-of-highly-polar-compounds-in-plasma-by-reversed-phase-liquid-chromatography-high-resolution-mass-spectrometry
#16
Elena Sánchez-López, Antonio L Crego, María Luisa Marina
Amino acids and related compounds are paramount analytes which are involved in numerous metabolic pathways. Most of these compounds are unable to be retained on Liquid Chromatography with Reversed-Phase stationary phases due to their high hydrophilic character. An interesting strategy is to reduce their polarity through their derivatization with a labelling reagent, such as the commercially available 9-fluorenylmethyloxycarbonyl (FMOC) which forms stable complexes with primary and secondary amine moieties rapidly...
February 15, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28238261/lipidomic-evaluation-of-aryl-hydrocarbon-receptor-mediated-hepatic-steatosis-in-male-and-female-mice-by-2-3-7-8-tetrachlorodibenzo-p-dioxin
#17
Rance Nault, Kelly A Fader, Todd A Lydic, Timothy R Zacharewski
The environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces hepatic steatosis mediated by the aryl hydrocarbon receptor. To further characterize TCDD-elicited hepatic lipid accumulation, mice were gavaged with TCDD every 4 days for 28 days. Liver samples were examined using untargeted lipidomics with structural confirmation of lipid species by targeted high-resolution MS/MS and data were integrated with complementary RNA-Seq analyses. Approximately 936 unique spectral features were detected, of which 379 were confirmed as unique lipid species...
February 27, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28238222/calcium-and-phosphorus-supplementation-of-human-milk-for-preterm-infants
#18
REVIEW
Jane E Harding, Jess Wilson, Julie Brown
BACKGROUND: Preterm infants are born with low skeletal stores of calcium and phosphorus. Preterm human milk provides insufficient calcium and phosphorus to meet the estimated needs of preterm infants for adequate growth. Supplementation of human milk with calcium and phosphorus may improve growth and development of preterm infants. OBJECTIVES: To determine whether addition of calcium and phosphorus supplements to human milk leads to improved growth and bone metabolism of preterm infants without significant adverse effects...
February 26, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28238189/retinal-neurodegeneration-in-patients-with-type-1-diabetes-mellitus-the-role-of-glycemic-variability
#19
Fabiana Picconi, Mariacristina Parravano, Dorina Ylli, Patrizio Pasqualetti, Sara Coluzzi, Ilaria Giordani, Ilaria Malandrucco, Davide Lauro, Fabio Scarinci, Paola Giorno, Monica Varano, Simona Frontoni
AIMS: Recent studies have identified neuroretinal abnormalities in persons affected by diabetes mellitus, before the onset of microvascular alterations. However, the role of glycemic variability (GV) on early retinal neurodegeneration is still not clarified. METHODS: To explore the relationship between glycemic control and neuroretinal characteristics, 37 persons with Type 1 diabetes mellitus (Type 1 DM) divided into two groups with no signs (noRD) and with mild non-proliferative diabetic retinopathy (NPDR) compared to 13 healthy control participants (C) were recruited...
February 25, 2017: Acta Diabetologica
https://www.readbyqxmd.com/read/28237843/characterization-of-energy-and-neurotransmitter-metabolism-in-cortical-glutamatergic-neurons-derived-from-human-induced-pluripotent-stem-cells-a-novel-approach-to-study-metabolism-in-human-neurons
#20
Blanca I Aldana, Yu Zhang, Maria Fog Lihme, Lasse K Bak, Jørgen E Nielsen, Bjørn Holst, Poul Hyttel, Kristine K Freude, Helle S Waagepetersen
Alterations in the cellular metabolic machinery of the brain are associated with neurodegenerative disorders such as Alzheimer's disease. Novel human cellular disease models are essential in order to study underlying disease mechanisms. In the present study, we characterized major metabolic pathways in neurons derived from human induced pluripotent stem cells (hiPSC). With this aim, cultures of hiPSC-derived neurons were incubated with [U-(13)C]glucose, [U-(13)C]glutamate or [U-(13)C]glutamine. Isotopic labeling in metabolites was determined using gas chromatography coupled to mass spectrometry, and cellular amino acid content was quantified by high-performance liquid chromatography...
February 22, 2017: Neurochemistry International
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