keyword
https://read.qxmd.com/read/38390807/plentiplex%C3%A2-myd88-waldenstr%C3%A3-m-lymphoma-qpcr-assay-a-highly-sensitive-method-for-detection-of-myd88-l265p-mutation
#21
JOURNAL ARTICLE
Marcello Viscovo, Mia de Laurent Clemmensen, Federica Fosso, Elena Maiolo, Francesco Autore, Luca Laurenti, Stefan Hohaus, Patrizia Chiusolo
INTRODUCTION: Agarose gel-based conventional and real-time allele-specific polymerase chain reaction (AS-PCR) assays are currently used for sensitive detection and quantification of MYD88 L265P mutation. Visual inspection of an agarose gel can often be ambiguous. We propose a new allele-specific quantification PCR (AS-qPCR) assay, PlentiPlex™ MYD88 Waldenström lymphoma qPCR assay, that uses Intercalating Nucleic Acid (INA®) technology for increased affinity and specificity...
February 23, 2024: International Journal of Laboratory Hematology
https://read.qxmd.com/read/38389258/serum-protein-electrophoresis-in-european-mink-mustela-lutreola-reference-intervals-and-comparison-of-agarose-gel-electrophoresis-and-capillary-zone-electrophoresis
#22
JOURNAL ARTICLE
Sergio Villanueva-Saz, María Del Carmen Aranda, María de Los Ángeles Jiménez, Paloma Jimena de Andrés, Maite Verde, María Climent, María Eugenia Lebrero Berna, Diana Marteles Aragüés, Antonio Fernández
BACKGROUND: Knowledge of reference intervals for blood analytes, including serum protein fractions, is of great importance for the identification of infectious and inflammatory diseases and is often lacking in wild animal species. MATERIAL AND METHODS: Serum samples were obtained from European minks enrolled in the breeding program (n = 55). Agarose gel electrophoresis (AGE) and capillary zone electrophoresis (CZE) were used to separate and identify protein fractions...
December 2024: Veterinary Quarterly
https://read.qxmd.com/read/38383334/hsa_circ_0005397-promotes-hepatocellular-carcinoma-progression-through-eif4a3
#23
JOURNAL ARTICLE
Liu-Xia Yuan, Mei Luo, Ruo-Yu Liu, Hui-Xuan Wang, Lin-Ling Ju, Feng Wang, Ya-Li Cao, Zhong-Cheng Wang, Lin Chen
PURPOSE: The purpose of this study was to explore the expression and potential mechanism of hsa_circ_0005397 in hepatocellular carcinoma progression. METHODS: Quantitative reverse transcription-polymerase chain reaction(qRT-PCR) was used to measure the expression level of hsa_circ_0005397 and EIF4A3 from paired HCC tissues and cell lines. Western Blot (WB) and immunohistochemistry (IHC) were used to verify the protein level of EIF4A3. The specificity of primers was confirmed by agarose gel electrophoresis...
February 21, 2024: BMC Cancer
https://read.qxmd.com/read/38382175/allium-cepa-tests-exploring-bleomycin-induced-cyto-genotoxicity-and-altered-cell-cycle-kinetics-in-root-tips-meristematic-cells
#24
JOURNAL ARTICLE
Ria Das, Sanjib Ray
Bleomycin, commonly employed in treating Hodgkin's lymphoma and testicular cancer, is associated with significant pulmonary toxicity. While various studies have assessed the toxic impact of chemotherapeutic agents on aquatic and terrestrial environments, limited data exist on bleomycin's effects, especially concerning higher plants. To address this gap, we utilized the Allium cepa assays, renowned for evaluating chemical and biochemical agents' toxic effects, to investigate bleomycin's impact on the terrestrial ecosystem...
February 9, 2024: Mutation Research
https://read.qxmd.com/read/38370775/heat-conduction-simulation-of-chondrocyte-embedded-agarose-gels-suggests-negligible-impact-of-viscoelastic-dissipation-on-temperature-change
#25
Erik Myers, Molly Piazza, Mark Owkes, Ronald K June
Agarose is commonly used for 3D cell culture and to mimic the stiffness of the pericellular matrix of articular chondrocytes. Although it is known that both temperature and mechanical stimulation affect the metabolism of chondrocytes, little is known about the thermal properties of agarose hydrogels. Thermal properties of agarose are needed to analyze potential heat production by chondrocytes induced by various experimental stimuli (carbon source, cyclical compression, etc). Utilizing ASTM C177, a custom-built thermal conductivity measuring device was constructed and used to calculate the thermal conductivity of 4...
February 9, 2024: bioRxiv
https://read.qxmd.com/read/38370737/unfolded-von-willebrand-factor-binds-protein-s-and-reduces-anticoagulant-activity
#26
Martha M S Sim, Molly Y Mollica, Hammodah R Alfar, Melissa Hollifield, Dominic W Chung, Xiaoyun Fu, Siva Gandhapudi, Daniëlle M Coenen, Kanakanagavalli Shravani Prakhya, Dlovan F D Mahmood, Meenakshi Banerjee, Chi Peng, Xian Li, Alice C Thornton, James Z Porterfield, Jamie L Sturgill, Gail A Sievert, Marietta Barton-Baxter, Ze Zheng, Kenneth S Campbell, Jerold G Woodward, José A López, Sidney W Whiteheart, Beth A Garvy, Jeremy P Wood
Protein S (PS), the critical plasma cofactor for the anticoagulants tissue factor (TF) pathway inhibitor (TFPI) and activated protein C (APC), circulates in two functionally distinct pools: free (anticoagulant) or bound to complement component 4b-binding protein (C4BP) (anti-inflammatory). Acquired free PS deficiency is detected in several viral infections, but its cause is unclear. Here, we identified a shear-dependent interaction between PS and von Willebrand Factor (VWF) by mass spectrometry. Consistently, plasma PS and VWF comigrated in both native and agarose gel electrophoresis...
February 9, 2024: bioRxiv
https://read.qxmd.com/read/38369336/brain-structures-in-a-human-embryo-imaged-with-mr-microscopy
#27
JOURNAL ARTICLE
Kazuki Kunieda, Kazuyuki Makihara, Shigehito Yamada, Masayuki Yamaguchi, Takashi Nakamura, Yasuhiko Terada
PURPOSE: To delineate brain microstructures in human embryos during the formation of the various major primordia by MR microscopy, with different contrasts appropriate for each target. METHODS: We focused mainly on the internal structures in the cerebral cortex and the accessory nerves of the brain. To find appropriate sequence parameters, we measured nuclear magnetic resonance (NMR) parameters and created kernel density plots of T1 and T2 values. We performed T1-weighted gradient echo imaging with parameters similar to those used in the previous studies...
February 16, 2024: Magnetic Resonance in Medical Sciences: MRMS
https://read.qxmd.com/read/38367540/different-behavior-of-ferguson-plot-between-agarose-and-polyacrylamide-gels
#28
JOURNAL ARTICLE
Yui Tomioka, Teruo Akuta, Masao Tokunaga, Tsutomu Arakawa
In this study, we conducted Ferguson plot analyses using both agarose and polyacrylamide gels in native electrophoresis and SDS-PAGE. The results revealed intriguing differences in the behavior of bovine serum albumin (BSA) and other model proteins. Specifically, BSA exhibited Ferguson plot slopes that were dependent on the oligomer size in agarose native gel electrophoresis, while such size-dependent behavior was not observed in native-PAGE or SDS-PAGE. These findings suggest that Ferguson plot analysis is a suitable approach when using agarose gel under the electrophoretic conditions employed in this study...
February 13, 2024: Biophysical Chemistry
https://read.qxmd.com/read/38365802/impact-of-artisanal-refining-activities-on-bacterial-diversity-in-a-niger-delta-fallow-land
#29
JOURNAL ARTICLE
Emmanuel Oliver Fenibo, Rosina Nkuna, Tonderayi Matambo
Hydrocarbon pollution is a major ecological problem facing oil-producing countries, especially in the Niger Delta region of Nigeria. In this study, a site that had been previously polluted by artisanal refining activity was investigated using 16S rRNA Illumina high-throughput sequencing technology and bioinformatics tools. These were used to investigate the bacterial diversity in soil with varying degrees of contamination, determined with a gas chromatography-flame ionization detector (GC-FID). Soil samples were collected from a heavily polluted (HP), mildly polluted (MP), and unpolluted (control sample, CS) portion of the study site...
February 16, 2024: Scientific Reports
https://read.qxmd.com/read/38363412/occurrence-of-existing-bcr-abl-baseline-mutations-and-associated-haplotype-nmr-among-cml-patients-with-diverse-im-response-a-hospital-based-study-from-north-east-india
#30
JOURNAL ARTICLE
Gautam Hazarika, Manash Jyoti Kalita, Partha Pratim Das, Simanta Kalita, Kalpajit Dutta, Lipika Lahkar, Anjanjyoti Rajkonwar, Mohammed Ghaznavi Idris, Vinotsale Khamo, Giriraj Kusre, Subhash Medhi
Highly polymorphic BCR-ABL kinase domains have been reported to harbor more than a hundred mutations, and among these, 40-60% have been identified as influencers of imatinib mesylate (IM) resistance. The emergence of IM resistance poses a significant challenge in the management of Chronic Myeloid Leukemia (CML). M351T (rs121913457), E255K (rs387906517), and Y253H (rs121913461) are of particular clinical significance due to their association with high-level imatinib resistance. This study was conducted to investigate the potential role of three significant SNPs in CML progression due to IM resistance...
February 16, 2024: Biochemical Genetics
https://read.qxmd.com/read/38359063/antioxidant-and-antiproliferative-effect-of-a-glycosaminoglycan-extract-from-rapana-venosa-marine-snail
#31
JOURNAL ARTICLE
Alexandra Gaspar-Pintiliescu, Laura M Stefan, Elena Mihai, Catalina Sanda, Vasile S Manoiu, Daniela Berger, Oana Craciunescu
Marine glycosaminoglycans (GAG) isolated from different invertebrates, such as molluscs, starfish or jellyfish, have been described as unique molecules with important pharmacological applications. Scarce information is available on GAG extract from Rapana venosa marine snail. The aim of this study was to isolate a GAG extract from R. venosa marine snail and to investigate its physicochemical, antioxidant and antiproliferative properties for further biomedical use. The morphology, chemical and elemental composition of the extract were established as well as the sulfate content and N- to O-sulfation ratio...
2024: PloS One
https://read.qxmd.com/read/38356693/-in-vitro-gadolinium-retro-microdialysis-in-agarose-gel-a-human-brain-phantom-study
#32
JOURNAL ARTICLE
Chisomo Zimphango, Marius O Mada, Stephen J Sawiak, Susan Giorgi-Coll, T Adrian Carpenter, Peter J Hutchinson, Keri L H Carpenter, Matthew G Stovell
RATIONALE AND OBJECTIVES: Cerebral microdialysis is a technique that enables monitoring of the neurochemistry of patients with significant acquired brain injury, such as traumatic brain injury (TBI) and subarachnoid haemorrhage (SAH). Cerebral microdialysis can also be used to characterise the neuro-pharmacokinetics of small-molecule study substrates using retrodialysis/retromicrodialysis. However, challenges remain: (i) lack of a simple, stable, and inexpensive brain tissue model for the study of drug neuropharmacology; and (ii) it is unclear how far small study-molecules administered via retrodialysis diffuse within the human brain...
2024: Front Radiol
https://read.qxmd.com/read/38353618/easy-to-use-crispr-cas9-genome-editing-in-the-cultured-pacific-abalone-haliotis-discus-hannai
#33
JOURNAL ARTICLE
Ruohui Li, Yue Xu, Fucun Wu, Zhangjie Peng, Jiulin Chan, Linlin Zhang
The Pacific abalone is an important aquaculture shellfish and serves as an important model in basic biology study. However, the study of abalone is limited by lack of highly efficient and easy-to-use gene-editing tools. In this paper, we demonstrate efficient gene knockout in Pacific abalone using CRISPR-Cas9. We developed a highly effective microinjection method by nesting fertilized eggs in a low-concentration agarose gel. We identified the cilia developmental gene β-tubulin and light-sensitive transmembrane protein r-opsin as target genes and designed highly specific sgRNAs for modifying their genomic sequences...
February 2024: CRISPR Journal
https://read.qxmd.com/read/38351209/isoelectric-trapping-and-discrimination-of-histones-from-plasma-in-a-microfluidic-device-using-dehydrated-isoelectric-gate
#34
JOURNAL ARTICLE
Shadi Shahriari, Sreekant Damodara, P Ravi Selvaganapathy
Histones are basic proteins with an isoelectric point around 11. It has been shown that the level of plasma circulating histones increases significantly during sepsis, and circulating free histones are associated with sepsis severity and mortality. It was found that the median plasma total free histone concentration of sepsis ICU non-survivors is higher compared to survivors. Therefore, histone concentration can serve as a prognostic indicator and there is a need for a simple, low-cost, and rapid method for measuring histone levels...
February 13, 2024: Mikrochimica Acta
https://read.qxmd.com/read/38350650/development-of-a-novel-dgt-passive-sampler-for-measuring-cs-137-in-situ-in-marine-environments
#35
JOURNAL ARTICLE
Ahmed Elsenbawy, Jacqueline M Pates, Nariman H M Kamel, Tarek Morsi, Mohammed Mekewi, Ayman El-Gamal, Nabawia A Moussa, Hao Zhang
Cs-137 is the most released fission product in the marine environment. It is important to develop a robust in situ technique for its monitoring. The existing diffusive gradients in thin films (DGT) passive sampling techniques for in situ measurement of Cs+ have some limitations due to the ion competition and high pH of seawater. A new DGT sampler based on potassium zinc hexacyanoferrate (KZFCN) as a binding layer has been developed and investigated for the measurement of the time-weighted average concentration of Cs-137 in seawater...
February 13, 2024: Analytical Chemistry
https://read.qxmd.com/read/38350580/rational-design-of-agarose-dextran-composite-microspheres-with-tunable-core-shell-microstructures-for-chromatographic-application
#36
JOURNAL ARTICLE
Jinming Ye, Yi Ru, Huifen Weng, Liling Fu, Jun Chen, Fuquan Chen, Qiong Xiao, Anfeng Xiao
A new type of core-shell microsphere was prepared by a pre-crosslinking method, consisting of cross-linked agarose microspheres as the core and agarose-dextran as the shell. After optimizing the preparation process, the microspheres with a uniform particle size were obtained and characterized using cryo-scanning electron microscopy to determine their surface and cross-sectional morphology. Results from flow rate-pressure and chromatographic performance tests showed that the core-shell agarose microspheres were supported by the core microspheres and composed of composite polysaccharides, forming an interpenetrating polymer network structure as a hard shell...
February 11, 2024: International Journal of Biological Macromolecules
https://read.qxmd.com/read/38345638/enhancement-of-%C3%AE-galactosidase-production-using-novel-actinoplanes-utahensis-b1-strain-sequential-optimization-and-purification-of-enzyme
#37
JOURNAL ARTICLE
K Balumahendra, T C Venkateswarulu, D John Babu
α-Galactosidase is an important exoglycosidase belonging to the hydrolase class of enzymes, which has therapeutic and industrial potential. It plays a crucial role in hydrolyzing α-1,6 linked terminal galacto-oligosaccharide residues such as melibiose, raffinose, and branched polysaccharides such as galacto-glucomannans and galactomannans. In this study, Actinoplanes utahensis B1 was explored for α-galactosidase production, yield improvement, and activity enhancement by purification. Initially, nine media components were screened using the Plackett-Burman design (PBD)...
February 12, 2024: World Journal of Microbiology & Biotechnology
https://read.qxmd.com/read/38344063/quality-control-method-and-device-for-producing-agarose-micropads
#38
JOURNAL ARTICLE
Martin Vo, Lance Kuo-Esser, Mauricio Dominguez, Julia Driggers, Kennedy Kuchinski, Sheny Perez, Taylor Luck, The Tran, Thang Tran, David Gerberry, Hanna Wetzel, Wilber Escorcia
In brightfield and fluorescence microscopy, capturing images that show well-focused and immobile microorganisms can be challenging. An agarose-based gel pad reduces the variability of results, especially in conditions like uneven specimen staging, variable fluid dynamics, and Brownian motion that plague conventional wet mount setups. To correct these discrepancies during image acquisition, we analyzed three micropad preparation setups. We tested the quality and consistency of pads and images resulting from each setup...
2024: microPublication. Biology
https://read.qxmd.com/read/38334173/use-of-therapeutic-plasma-exchange-to-remove-lipoprotein-x-in-a-patient-with-vanishing-bile-duct-syndrome-presenting-with-cholestasis-pseudohyponatremia-and-hypercholesterolemia-a-case-report-and-review-of-literature
#39
JOURNAL ARTICLE
Yujung Jung, Heather A Nelson, David Ming-Hung Lin
INTRODUCTION: Lipoprotein X (Lp-X) is an abnormal lipoprotein found in multiple disease conditions, including liver dysfunction and cholestasis. High Lp-X concentrations can interfere with some laboratory testing that may result in spurious results. The detection of Lp-X can be challenging, and there is currently a lack of consensus regarding the management of Lp-X other than treating the underlying disease. CASE PRESENTATION: A 42-year-old female with Hodgkin's lymphoma treated with dexamethasone, high dose cytarabine and cisplatin and vanishing bile duct syndrome confirmed by liver biopsy presented with cholestasis, pseudohyponatremia (sodium, 113 mmol/L; reference range 136-146 mmL/L; serum osmolality, 303 mOsm/kg), and hypercholesterolemia (> 2800 mg/dL, reference range < 200 mg/dL)...
February 2024: Journal of Clinical Apheresis
https://read.qxmd.com/read/38332584/nonspecific-membrane-matrix-interactions-influence-diffusivity-of-lipid-vesicles-in-hydrogels
#40
JOURNAL ARTICLE
Nicky W Tam, Otto Schullian, Amaia Cipitria, Rumiana Dimova
The diffusion of extracellular vesicles and liposomes in vivo is affected by different tissue environmental conditions and is of great interest in the development of liposome-based therapeutics and drug-delivery systems. Here, we use a bottom-up biomimetic approach to better isolate and study steric and electrostatic interactions and their influence on the diffusivity of synthetic large unilamellar vesicles in hydrogel environments. Single-particle tracking of these extracellular vesicle-like particles in agarose hydrogels as an extracellular matrix model shows that membrane deformability and surface charge affect the hydrogel pore spaces that vesicles have access to, which determines overall diffusivity...
February 7, 2024: Biophysical Journal
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