Martha M S Sim, Molly Y Mollica, Hammodah R Alfar, Melissa Hollifield, Dominic W Chung, Xiaoyun Fu, Siva Gandhapudi, Daniëlle M Coenen, Kanakanagavalli Shravani Prakhya, Dlovan F D Mahmood, Meenakshi Banerjee, Chi Peng, Xian Li, Alice C Thornton, James Z Porterfield, Jamie L Sturgill, Gail A Sievert, Marietta Barton-Baxter, Ze Zheng, Kenneth S Campbell, Jerold G Woodward, José A López, Sidney W Whiteheart, Beth A Garvy, Jeremy P Wood
Protein S (PS), the critical plasma cofactor for the anticoagulants tissue factor (TF) pathway inhibitor (TFPI) and activated protein C (APC), circulates in two functionally distinct pools: free (anticoagulant) or bound to complement component 4b-binding protein (C4BP) (anti-inflammatory). Acquired free PS deficiency is detected in several viral infections, but its cause is unclear. Here, we identified a shear-dependent interaction between PS and von Willebrand Factor (VWF) by mass spectrometry. Consistently, plasma PS and VWF comigrated in both native and agarose gel electrophoresis...
February 9, 2024: bioRxiv