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https://www.readbyqxmd.com/read/28721850/amphotericin-b-induces-apoptosis-like-programmed-cell-death-in-naegleria-fowleri-and-naegleria-gruberi
#1
Roberto Cárdenas-Zúñiga, Angélica Silva-Olivares, José D' Artagnan Villalba-Magdaleno, Virginia Sánchez-Monroy, Jesús Serrano-Luna, Mineko Shibayama
Naegleria fowleri and Naegleria gruberi belong to the free-living amoebae group. It is widely known that the non-pathogenic species N. gruberi is usually employed as a model to describe molecular pathways in this genus, mainly because its genome has been recently described. However, N. fowleri is an aetiological agent of primary amoebic meningoencephalitis, an acute and fatal disease. Currently, the most widely used drug for its treatment is amphotericin B (AmB). It was previously reported that AmB has an amoebicidal effect in both N...
July 18, 2017: Microbiology
https://www.readbyqxmd.com/read/28721511/molecular-and-phylogenetic-characterization-of-bovine-coronavirus-virus-isolated-from-dairy-cattle-in-central-region-thailand
#2
Kanokwan Singasa, Taweesak Songserm, Preeda Lertwatcharasarakul, Pipat Arunvipas
Bovine coronavirus (BCoV) is involved mainly in enteric infections in cattle. This study reports the first molecular detection of BCoV in a diarrhea outbreak in dairy cows in the Central Region, Thailand. BCoV was molecularly detected from bloody diarrheic cattle feces by using nested PCR. Agarose gel electrophoresis of three diarrheic fecal samples yielded from the 25 samples desired amplicons that were 488 base pairs and sequencing substantiated that have BCoV. The sequence alignment indicated that nucleotide and amino acid sequences, the three TWD isolated in Thailand, were more quite homologous to each other (amino acid at position 39 of TWD1, TWD3 was proline, but TWD2 was serine) and closely related to OK-0514-3strain (virulent respiratory strain; RBCoV)...
July 18, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28721300/extracellular-matrix-alterations-in-the-peyronie-s-disease
#3
Marcelo Silva Watanabe, Thérèse Rachel Theodoro, Natália Lima Coelho, Aline Mendes, Monica Luzia Pereira Leonel, Ana Maria Mader, Helena Bonciani Nader, Sidney Glina, Maria Aparecida Silva Pinhal
Peyronie's disease is characterized by fibrous plaque formation of the tunica albuginea, causing penile deformity and fertility problems. The aim of the present study was to investigate alterations in the extracellular matrix in Peyronie's disease. The study used tissues collected by surgical procedure from individuals that presented a well-established disease, while control samples were obtained by biopsies of fresh cadavers. Immunohistochemistry analysis followed by digital quantification was performed to evaluate TGF-β, heparanases and metalloproteinases (MMPs)...
July 2017: Journal of Advanced Research
https://www.readbyqxmd.com/read/28715381/modified-terminal-restriction-fragment-analysis-for-quantifying-telomere-length-using-in-gel-hybridization
#4
Frank J Jenkins, Charles M Kerr, Elise Fouquerel, Dana H Bovbjerg, Patricia L Opresko
There are several different techniques for measuring telomere length, each with their own advantages and disadvantages. The traditional approach, Telomere Restriction Fragment (TRF) analysis, utilizes a DNA hybridization technique whereby genomic DNA samples are digested with restriction enzymes, leaving behind telomere DNA repeats and some sub-telomeric DNA. These are separated by agarose gel electrophoresis, transferred to a filter membrane and hybridized to oligonucleotide probes tagged with either chemiluminescence or radioactivity to visualize telomere restriction fragments...
July 10, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28714559/how-we-make-dna-origami
#5
Klaus F Wagenbauer, Floris A S Engelhardt, Evi K Stahl, Vera K Hechtl, Pierre Stömmer, Fabian Seebacher, Letizia Meregalli, Philip Ketterer, Thomas Gerling, Hendrik Dietz
DNA ORIGAMI has attracted substantial attention since its invention ten years ago due to the seemingly infinite possibilities that it affords for creating customized nanoscale objects. While the basic concept of DNA origami is easy to understand, using custom DNA origami in practical applications requires detailed know-how for designing and producing the particles with sufficient quality, and preparing them at appropriate concentrations with the necessary degree of purity in custom environments. Such know-how is not readily available for newcomers to the field, thus slowing down the rate at which new applications outside the field of DNA nanotechnology may emerge...
July 17, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28711992/oleuropein-and-hydroxytyrosol-inhibit-the-n-formyl-methionyl-leucyl-phenylalanine-induced-neutrophil-degranulation-and-chemotaxis-via-akt-p38-and-erk1-2-map-kinase-inhibition
#6
Samia Bedouhene, Farida Moulti-Mati, Pham My-Chan Dang, Jamel El-Benna
PURPOSE: Oleuropein and hydroxytyrosol are polyphenols that are extracted from olives and are major biological active components of olives and olive oil. Oleuropein and hydroxytyrosol exhibit interesting pharmacological effects on cells, and have been shown to have many health benefits such as anti-inflammatory effects. These effects were mainly attributed to their ability to scavenge the reactive oxygen species (ROS) produced by phagocytes such as neutrophils. The aim of this study was to investigate the effect of oleuropein and hydroxytyrosol on other neutrophil functions...
July 15, 2017: Inflammopharmacology
https://www.readbyqxmd.com/read/28701019/decontamination-of-mikania-glomerata-leaves-by-gamma-irradiation-coumarin-determination-by-hplc-dad-microbiological-control-and-genotoxicological-studies
#7
Marcos Martins Gouvêa, Samara Cristina Ferreira-Machado, Thalita Martins da Silva, Julien Silva Lima Lima, Cláudio Sérgio Corrêa Lau, Luciana Maria Ramires Esper, Samanta Cardozo Mourão, Elizabeth Valverde Macedo, Helio de Carvalho Vital, Edson Ramos Andrade, Annibal Duarte Pereira Netto, Flávia Ferreira de Carvalho Marques, Carlos Augusto de Freitas Peregrino
Gamma irradiation as a decontaminating physical agent could be an important tool in the production chain of herbal medicines by improving the microbiological quality of raw materials and the safety of final products. This study was undertaken to investigate the genotoxic potential and eventual chemical modifications of a batch of Mikania glomerata raw material decontaminated by different doses of gamma irradiation (2.0, 3.5, and 5.0 kGy), using a cesium-137 source. DNA damage was assessed in vitro by agarose gel electrophoresis in regard to double-chain breaks of plasmid pUC 9...
July 12, 2017: Planta Medica
https://www.readbyqxmd.com/read/28696055/practices-and-exploration-on-competition-of-molecular-biological-detection-technology-among-students-in-food-quality-and-safety-major
#8
Yaning Chang, Yuke Peng, Pengfei Li, Yingping Zhuang
With the increasing importance in the application of the molecular biological detection technology in the field of food safety, strengthening education in molecular biology experimental techniques is more necessary for the culture of the students in food quality and safety major. However, molecular biology experiments are not always in curricula of Food quality and safety Majors. This paper introduced a project "competition of molecular biological detection technology for food safety among undergraduate sophomore students in food quality and safety major", students participating in this project needed to learn the fundamental molecular biology experimental techniques such as the principles of molecular biology experiments and genome extraction, PCR and agarose gel electrophoresis analysis, and then design the experiments in groups to identify the meat species in pork and beef products using molecular biological methods...
July 8, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/28692132/plasma-protein-electrophoresis-as-a-prognostic-indicator-in-aspergillus-species-infected-gentoo-penguins-pygoscelis-papua-papua
#9
Adam D Naylor, Simon J Girling, Donna Brown, Claire G Crompton, Romain Pizzi
BACKGROUND: Avian aspergillosis presents a significant threat to captive penguin populations. Currently, a lack of objective prognostic factors limits disease staging, objective reassessment throughout treatment, comparative evaluation of treatment regimes, and appropriate timing of euthanasia. OBJECTIVE: The study objective was to investigate absolute and relative plasma protein fractions by agarose gel electrophoresis (EPH) as predictors of survival in Gentoo penguins (Pygoscelis papua papua) under treatment for aspergillosis...
July 10, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28692121/molecular-analysis-of-the-gstt1-gene-polymorphism-in-patients-with-clinical-manifestation-of-atherosclerosis
#10
J V M Martins, D A Rodrigues, K S F Silva, I R Costa, M H Lagares, F L Campedelli, A M Barbosa, M P Morais, K K V O Moura
Atherosclerosis is a chronic inflammatory disease formed by the accumulation of lipids in the innermost layer and large-caliber artery (tunica intima). This accumulation, along with platelet factors, stimulates the proliferation of muscle cells in this region. Over than 400 genes may be related to the pathology since they regulate endothelial function, coagulation, inflammation, metabolism of amino acids, lipids, and carbohydrates. Glutathione S-transferases (GST) are enzymes that catalyze the polymorphic detoxification of metabolites produced by oxidative stress within the cells, which is induced by reactive oxygen species...
July 6, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28690796/a-novel-method-of-caenophidian-snake-sex-identification-using-molecular-markers-based-on-two-gametologous-genes
#11
Nararat Laopichienpong, Panupong Tawichasri, Lawan Chanhome, Rattanin Phatcharakullawarawat, Worapong Singchat, Attachai Kantachumpoo, Narongrit Muangmai, Sunutcha Suntrarachun, Kazumi Matsubara, Surin Peyachoknagul, Kornsorn Srikulnath
Sex identification provides important information for ecological and evolutionary studies, as well as benefiting snake conservation management. Traditional methods such as cloacal probing or cloacal popping are counterproductive for sex identification concerning very small species, resulting in difficulties in the management of their breeding programs. In this study, the nucleotide sequences of gametologous genes (CTNNB1 and WAC genes) were used for the development of molecular sexing markers in caenophidian snakes...
July 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28690181/hydrogen-peroxide-agarose-gels-for-electrophoretic-analysis-of-rna
#12
Renu Pandey, Daman Saluja
Efficient electrophoretic separation of isolated total RNA utilizes chemicals and agents to aid in nuclease free environment. However cost, extensive pre-run processing protocols as well as toxic byproducts limit the usage of such protocols. Moreover, these treatments affect the overall electrophoretic results by altering the conductivity of the running buffer and weaken the gel strength. We here provide a protocol for RNA visualization that obviates these shortcomings by preparation of agarose gel with hydrogen peroxide using the regular TAE buffer...
July 6, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28671653/protein-trna-agarose-gel-retardation-assays-for-the-analysis-of-the-n-6-threonylcarbamoyladenosine-tcda-function
#13
Francisco J Fernández, Sara Gómez, Sergio Navas-Yuste, Miguel López-Estepa, M Cristina Vega
We demonstrate methods for the expression and purification of tRNA(UUU) in Escherichia coli and the analysis by gel retardation assays of the binding of tRNA(UUU) to TcdA, an N(6)-threonylcarbamoyladenosine (t(6)A) dehydratase, which cyclizes the threonylcarbamoyl side chain attached to A37 in the anticodon stem loop (ASL) of tRNAs to cyclic t(6)A (ct(6)A). Transcription of the synthetic gene encoding tRNA(UUU) is induced in E. coli with 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) and the cells containing tRNA are harvested 24 h post-induction...
June 21, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28668699/cxcr2-1208-ct-genotype-may-predict-earlier-clinical-stage-at-diagnosis-in-patients-with-prostate-cancer
#14
Juliana M Franz, Pâmela Portela, Patricia H Salim, Milton Berger, Luiz Fernando Jobim, Rafael Roesler, Mariana Jobim, Gilberto Schwartsmann
Interleukin-8 (IL-8) is an angiogenic CXC chemokine that plays an important role in both the development and progression of several human malignancies including prostate cancer (PC). A single nucleotide polymorphism (SNP) at -251 upstream of the transcriptional start site of the IL-8 gene has been shown to influence its production. The effects of IL-8 are mediated by two highly related chemokine receptors, CXCR1 and CXCR2. The present study investigated the influence of the IL-8 and CXCR2 gene variation on susceptibility and clinicopathological characteristics of PC in a group of Brazilian subjects...
June 29, 2017: Cytokine
https://www.readbyqxmd.com/read/28664742/delivery-of-hiv-1-nef-linked-to-heat-shock-protein-27-using-a-cationic-polymer-is-more-effective-than-cationic-lipid-in-mammalian-cells
#15
A Milani, A Bolhassani, M Heshmati
BACKGROUND: Different adjuvants and delivery systems have been used to enhance the potency of DNA vaccines against viral diseases. Among them, heat shock proteins (HSPs) are stress proteins that have multiple roles such as chaperon activity and anti-apoptotic and adjuvant properties. The goal of this study was to compare the expression of HIV-1 Nef, Hsp27 and Hsp27-Nef genes transfected in HEK-293T mammalian cells by TurboFect and Lipofectamine as a cationic polymer and lipid, respectively...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/28664367/a-quick-and-effective-in-house-method-of-dna-purification-from-agarose-gel-suitable-for-sequencing
#16
Obrador-Sánchez José Abraham, Tzec-Sima Miguel, Higuera-Ciapara Inocencio, Canto-Canché Blondy
Sequencing of DNA fragments (e.g., ITS, 16S, 18S, particular genes, and molecular markers) is increasingly required in studies on microbial diversity, microbial genetic population and phylogeny, sequencing of alleles, and searching for SNPs, among others. The cost of obtaining these DNAs, in quantity and quality for sequencing, is high as it involves special kits to recover DNA from gel after PCR, or the cloning and purification of plasmids with commercial kits. Genetic population and other studies require the analyses of many samples, and therefore, the high cost represents an obstacle for carrying out such projects in countries where there is great biodiversity, such as the tropical and subtropical developing countries, where funds are limited...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28664141/induction-of-apoptosis-by-dae-hwang-mok-dan-tang-in-hct-116-colon-cancer-cells-through-activation-of-caspases-and-inactivation-of-the-phosphatidylinositol-3-kinase-akt-signaling
#17
Cheol Park, Su Hyun Hong, Yung Hyun Choi
BACKGROUND: Dae-Hwang-Mok-Dan-Tang (DHMDT), a traditional Korean medicine, contains five species of medicinal plants and has been used to treat patients with digestive tract cancer for hundreds of years; however, its anticancer mechanism is poorly understood. In the present study, we investigated the proapoptotic effects of DHMDT in human colon cancer HCT-116 cells. METHODS: Cytotoxicity was evaluated using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay...
June 2017: Integrative medicine research
https://www.readbyqxmd.com/read/28651169/anticancer-activity-of-hydroxy-and-sulfonamide-azobenzene-platinum-ii-complexes-in-cisplatin-resistant-ovarian-cancer-cells
#18
Katia G Samper, Sierra C Marker, Pau Bayón, Samantha N MacMillan, Ivan Keresztes, Òscar Palacios, Justin J Wilson
The syntheses of three platinum(II) complexes bearing sulfonamide- ( (E)-2-(4-methylphenylsulfonamido)-2',6'-difluoroazobenzene, HL1) and hydroxy-azo-2,6-difluorobenzene ((E)-2-((2,6-difluorophenyl)diazenyl)phenol, HL2) bidentate ligands is described. These complexes, [Pt(L1)(DMSO)Cl] (1), [Pt(L2)(DMSO)Cl] (2), and [Pt(L2)2] (3), were characterized by multinuclear NMR spectroscopy, mass spectrometry, and X-ray crystallography. Despite bearing azobenzene functional groups, none of the three complexes undergo photoisomerization...
September 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28649589/data-supporting-the-anticancer-activity-of-posterior-salivary-gland-psg-toxin-from-the-cuttlefish-sepia-pharaonis-ehrenberg-1831
#19
Ramachandran Karthik, Venkatesan Manigandan, Kumar Ebenezar, Raghavan Vijayashree, Ramachandran Saravanan
The data presented illustrated the in vitro anti-proliferative effect of the PSG toxin from the cuttlefish, Sepia pharaonis. The cytostatic potentials of the PSG toxin were determined by the lymphocyte migration inhibition assay. The PSG toxin (50 μg/ml) exhibited commendable inhibition of the migration of lymphocytes across the agarose gel matrix under the presence of lipopolysaccharide mitogen, with a mean migration index of 0.625. The cytotoxicity of the PSG toxin against selected cancer cell lines was determined using the MTT assay...
August 2017: Data in Brief
https://www.readbyqxmd.com/read/28648925/new-imidoyl-indazole-platinum-ii-complexes-as-potential-anticancer-agents-synthesis-evaluation-of-cytotoxicity-cell-death-and-experimental-theoretical-dna-interaction-studies
#20
Alan R Cabrera, Christian Espinosa-Bustos, Mario Faúndez, Jaime Meléndez, Pablo Jaque, Constantin G Daniliuc, Adam Aguirre, Rene S Rojas, Cristian O Salas
Four new neutral N,N imidoyl-indazole ligands (L1, L3, L6, L7) and six new Pt(II)-based complexes (C1-5 and C7) were synthesized and characterized by spectroscopic and spectrometric techniques. Additionally, compounds L6, L7, C3, C5 and C7 were analyzed using X-ray diffraction. An evaluation of cytotoxicity and cell death in vitro for both ligands and complexes was performed by colorimetric assay and flow cytometry, in four cancer cell lines and VERO cells as the control, respectively. Cytotoxicity and selectivity demonstrated by each compound were dependent on the cancer cell line assayed...
September 2017: Journal of Inorganic Biochemistry
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