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Agarose gel

Manuel Ramírez, Rocío Velázquez, Antonio López-Piñeiro, Belén Naranjo, Francisco Roig, Carlos Llorens
Viral M-dsRNAs encoding yeast killer toxins share similar genomic organization, but no overall sequence identity. The dsRNA full-length sequences of several known M-viruses either have yet to be completed, or they were shorter than estimated by agarose gel electrophoresis. High-throughput sequencing was used to analyze some M-dsRNAs previously sequenced by traditional techniques, and new dsRNAs from atypical killer strains of Saccharomyces cerevisiae and Torulaspora delbrueckii. All dsRNAs expected to be present in a given yeast strain were reliably detected and sequenced, and the previously-known sequences were confirmed...
September 19, 2017: Toxins
Adriana Patarroyo Vargas, Yaremis Meriño Cabrera, José Cola Zanuncio, Francelina Rocha, Wellington Garcia Campos, Maria Goreti de Almeida Oliveira
The kinetics of Anticarsia gemmatalis Hübner gut serine proteases with tripeptidyl substrates, and the enzymatic inhibition model using synthetic peptides Gor 3, Gor 4, and Gor 5 are described. The enzymes were purified on p-aminobenzamidine agarose affinity column in an FPLC system using electrophoresis with 12.5% polyacrylamide gel. Michaelis-Menten constants and the inhibition model were determined according to the Dixon methodology and Lineweaver-Burk's double reciprocal. The KM values and catalytic constants of peptide substrates show that A...
September 17, 2017: Protein and Peptide Letters
Yu Ting Chong, Marinah Mohd Ariffin, Norhayati Mohd Tahir, Saw Hong Loh
Electro-mediated microextraction (EMM) combined with micro-high performance liquid chromatography-ultraviolet detection was successfully developed for the determination of selected phenols, namely 4-chlorophenol (4CP), 2-nitrophenol (2NP) and 2,4-dichlorophenols (2,4 DCP) in water. A solvent-impregnated agarose gel disc was utilized as a solvent holder in this study. Under optimum extraction conditions, the method showed good linearity in the range of 0.1-250µgL(-1), 0.3-250µgL(-1) and 0.2-500µgL(-1) for 4CP, 2NP and 2,4 DCP, respectively with correlation coefficients of ≥ 0...
January 1, 2018: Talanta
Jianyin Huang, William W Bennett, Peter R Teasdale, Nadeeka Rathnayake Kankanamge, David T Welsh
A modified diffusive gradients in thin films (DGT) technique uses both a mixed binding layer (PrCH and A520E resins for NH4-N and NO3-N, respectively) and multiple binding layers (Metsorb binding layer for PO4-P overlying the mixed binding layer) for the simultaneous measurement of dissolved inorganic nitrogen (nitrate and ammonium) and phosphate in freshwater (INP-DGT). High uptake and elution efficiencies were determined for a mixed (PrCH/A520E) binding gel for dissolved inorganic nitrogen and an agarose-based Metsorb binding layer for PO4-P...
October 2, 2017: Analytica Chimica Acta
Shuzhen Wang, Yongliang Zheng, Fu Xiang, Shiming Li, Guliang Yang
Momordica charantia L., a vegetable crop with high nutritional value, has been used as an antimutagenic, antihelminthic, anticancer, antifertility, and antidiabetic agent in traditional folk medicine. In this study, the antifungal activity of M. charantia seed extract toward Fusarium solani L. was evaluated. Results showed that M. charantia seed extract effectively inhibited the mycelial growth of F. solani, with a 50% inhibitory rate (IC50) value of 108.934 μg/mL. Further analysis with optical microscopy and fluorescence microscopy revealed that the seed extract led to deformation of cells with irregular budding, loss of integrity of cell wall, as well as disruption of the fungal cell membrane...
October 2016: Journal of Food and Drug Analysis
A O Ibitayo, O B Afolabi, A J Akinyemi, T I Ojiezeh, K O Adekoya, O O Ojewunmi
The advent of Wi-Fi connected high technology devices in executing day-to-day activities is fast evolving especially in developing countries of the world and hence the need to assess its safety among others. The present study was conducted to investigate the injurious effect of radiofrequency emissions from installed Wi-Fi devices in brains of young male rats. Animals were divided into four equal groups; group 1 served as control while groups 2, 3, and 4 were exposed to 2.5 Ghz at intervals of 30, 45, and 60 consecutive days with free access to food and water ad libitum...
2017: BioMed Research International
Fatima Dahmani, Souad Benkirane, Jaafar Kouzih, Aziz Woumki, Hassan Mamad, Azlarab Masrar
Hemoglobinopathies are congenital disorders resultimg from hemoglobin abnormalities. Major forms are often severe, their management is difficult and associated with a great psychosocial impact on patients and their families. They are classified as rare diseases and are still insufficiently known by health professionals. This lack of knowledge is at the origin of diagnostic errors, delay in their management and therefore high morbidity and mortality rate for these patients. In 2008, the World Health Organization (WHO) has published data on hemoglobinopathies epidemiology: more than 330...
2017: Pan African Medical Journal
Jian-Wei Chen, Ning Shao, Yuchen Zhang, Yuanshou Zhu, Litao Yang, Sheng-Ce Tao
There is an urgent demand for affordable, rapid and easy-to-use technology to simultaneously detect many different DNA targets within one reaction. Conventional multiplex PCR is an effective methodology to simultaneously amplify different DNA targets. However, its multiplicity is limited due to the intrinsic interference and competition among primer pairs within one tube. Here, we present an easy multiplex PCR microchip system, which can simultaneously detect 54 targets. The design of the microchip is quite simple...
June 20, 2017: Yi Chuan, Hereditas
Nirmala Ganji, Vijay Kumar Chityala, Pradeep Kumar Marri, Rambabu Aveli, Vamsikrishna Narendrula, Sreenu Daravath, Shivaraj
Two new series of binary metal complexes [M(L(1))2] and [M(L(2))2] where, M=Cu(II), Ni(II) & Co(II) and L(1)=4-((3,4-dimethylisoxazol-5-ylimino)methyl)benzene-1,3-diol; L(2)=2-((3,4-dimethylisoxazol-5-ylimino)methyl)-5-methoxyphenol were synthesized and characterized by elemental analysis, (1)H NMR, (13)C NMR, FT-IR, ESI mass, UV-Visible, magnetic moment, ESR, SEM and powder XRD studies. Based on these results, a square planar geometry is assigned for all the metal complexes where the Schiff base acts as uninegatively charged bidentate chelating agent via the hydroxyl oxygen and azomethine nitrogen atoms...
August 31, 2017: Journal of Photochemistry and Photobiology. B, Biology
Huda H Al-Griw, Zena A Zraba, Salsabiel K Al-Muntaser, Marwan M Draid, Aisha M Zaidi, Refaat M Tabagh, Mohamed A Al-Griw
Efficient extraction of genomic DNA (gDNA) from biological materials found in harsh environments is the first step for successful forensic DNA profiling. This study aimed to evaluate two methods for DNA recovery from animal tissues (livers, muscles), focusing on the best storage temperature for DNA yield in term of quality, quantity, and integrity for use in several downstream molecular techniques. Six male Swiss albino mice were sacrificed, liver and muscle tissues (n=32) were then harvested and stored for one week in different temperatures, -20°C, 4°C, 25°C and 40°C...
2017: Open veterinary journal
Özden Tarı, Fatma Gümüş, Leyla Açık, Betül Aydın
The aim of this study was to synthesize and evaluate plasmid DNA interaction of new platinum(II) complexes with some 2-substituted benzimidazole derivatives as carrier ligands which may have potent anticancer activity and low toxicity. Twelve benzimidazole derivatives carrying indole, 2-/or 3-/or 4-methoxyphenyl, 4-methylphenyl, 3,4-dimethoxyphenyl, 3,4,5-trimethoxyphenyl, 4-methoxybenzyl, 3,4,5-trimethoxybenzyl, 3,4,5-trimethoxystyryl, 3,4,5-trimethoxybenzylthio or dimethylamino ethyl groups in their position 2 and twelve platinum(II) complexes with these carrier ligands were synthesized...
September 1, 2017: Bioorganic Chemistry
Mona S Larijani, Seyed M Sadat, Mehri Nikbin, Nasir Mohajel
METHODS: This case-control study, was performed on 136 blood samples based on 81 patients with chronic HCV genotypes 1 and 3 including 64 SVR positive and 17 negative and 55 healthy individual controls. DNA was isolated from the samples and the frequency of the polymorphism was analyzed using a PCR-RFLP method. Finally, the products were detected on 3.5% agarose gel electrophoresis. RESULTS: The analysis of the data for C/T polymorphism indicated that the CC genotype was found in 19 of 64 patients who achieved SVR, while the TT genotype was detected in 3 patients and SVR was achieved in 2...
September 1, 2017: Clinical Laboratory
Natalie Naidoo, Meenu Ghai, Kiresen Moodley, Lwamkilekele Mkhize, Lauren Martin, Sharon McFarlane, Stuart Rutherford
Ratoon stunt caused by bacterium Leifsonia xyli subsp. xyli (Lxx) results in substantial yield losses in sugarcane (Saccharum spp. L. hybrid). Since RS does not produce reliable symptoms in the field, laboratory-based techniques are necessary for detection. Loop-Mediated Isothermal Amplification (LAMP) assay overcomes the limitations of laboratory-based techniques which are costly, time consuming and cannot be used for near-field detection. A sensitive LAMP assay was developed to detect Lxx at 65°C in 30 min...
September 6, 2017: Letters in Applied Microbiology
Ruth Núñez-Flores, Deysi Cando, A Javier Borderías, Helena M Moreno
To address the effect of absence of NaCl on myosin heavy chain polymerization during two-step surimi gelation (different setting temperatures/times -5°C/24h and 30°C/30min-followed by heating at 90°C/30min) were considered. In gel samples made without salt (Lot A), no myosin heavy chain (MHC) polymerization was observed, only aggregation, as indicated by the electrophoresis in polyacrylamide/agarose gel profile. Moreover, these gels were characterized by weakly stabilized protein networks as denoted by the dynamic oscillatory measurement and FTIR analysis, resulting in poor quality gels...
January 15, 2018: Food Chemistry
Zidong Li, Erkin Seker
Advanced biomedical device coatings have shown significant promise in delivery of therapeutics (e.g., small-molecule drugs, proteins) for a wide range of medical interventions ranging from targeted cancer therapy to management of atherosclerosis. In order to accelerate the development of such coatings, there is a need for tools to investigate the loading capacity and release kinetics with high temporal resolution and in a variety of physiological conditions. To address this need, we report a microfluidic platform, where the coating on a substrate can be mounted onto the microchannel and the device can be configured in two physiologically-relevant modes: (i) flow-mode allows for monitoring the release from the coating in contact with a liquid flowing at a specific rate, modeling the case of a drug-eluting stent...
September 4, 2017: Lab on a Chip
Sergio M Carballo, Linda Haas, Christian G Krueger, Jess D Reed
In this work we characterize the interaction of cranberry (Vaccinium macrocarpon) proanthocyanidins (PAC) with bovine serum albumin (BSA) and hen egg-white lysozyme (HEL) and determine the effects of these complexes on macrophage activation and antigen presentation. We isolated PAC from cranberry and complexed the isolated PAC with BSA and HEL. The properties of the PAC-protein complexes were studied by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), gel electrophoresis and zeta-potential...
September 1, 2017: Food & Function
Chengshui Liao, Xiaoli Wang, Wenjing Tian, Mengke Zhang, Chunjie Zhang, Yinju Li, Tingcai Wu, Xiangchao Cheng
Although there are 125 predicted DNase Ⅱ-like family genes in the Trichinella spiralis genome, plancitoxin-1-like (Ts-Pt) contains the HKD motif, a typical conserved region of DNase Ⅱ, in N- and C-terminal. It is generally believed that histidine is the active site in DNase Ⅱ. To study the nuclease activity of recombinant Ts-Pt with mutations in the active site from T. spiralis, different fragments of the mutated Ts-Pt genes were cloned using overlap PCR technique and inserted into the expressing vector pET-28a(+), and transformed into Escherichia coli Rosseta (DE3)...
August 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
Matheus M Dos Santos, Alessandro S Prestes, Gabriel T de Macedo, Assis Ecker, Rômulo P Barcelos, Aline A Boligon, Diego Souza, Andreza F de Bem, João B T da Rocha, Nilda V Barbosa
ETNOPHARMACOLOGICAL RELEVANCE: Syzygium cumini (L.) Skeels is a plant widely used in folk medicine to treat diabetes mellitus (DM). The tea from its leaves is frequently used by diabetics for lowering hyperglycemia. There is a close relationship between DM and atherosclerosis, a chronic immuno-inflammatory disease, were the early stages encompass oxidative and glycative modifications in the structure of low density lipoprotein (LDL). AIM OF THIS STUDY: To investigate the potential protective effects of aqueous-leaf extract from Syzygium cumini (S...
August 26, 2017: Journal of Ethnopharmacology
Susanne Heid, Harald Unterweger, Rainer Tietze, Ralf P Friedrich, Bianca Weigel, Iwona Cicha, Dietmar Eberbeck, Aldo R Boccaccini, Christoph Alexiou, Stefan Lyer
Superparamagnetic iron oxide nanoparticles (SPIONs) have attracted great attention in many biomedical fields and are used in preclinical/experimental drug delivery, hyperthermia and medical imaging. In this study, biocompatible magnetite drug carriers, stabilized by a dextran shell, were developed to carry tissue plasminogen activator (tPA) for targeted thrombolysis under an external magnetic field. Different concentrations of active tPA were immobilized on carboxylated nanoparticles through carbodiimide-mediated amide bond formation...
August 24, 2017: International Journal of Molecular Sciences
Hilda Tiricz, Bettina Nagy, Györgyi Ferenc, Katalin Török, István Nagy, Dénes Dudits, Ferhan Ayaydin
Improving efficiency of oligonucleotide-directed mutagenesis (ODM) is a prerequisite for wide application of this gene-editing approach in plant science and breeding. Here we have tested histone deacetylase inhibitor treatments for induction of relaxed chromatin and for increasing the efficiency of ODM in cultured maize cells. For phenotypic assay we produced transgenic maize cell lines expressing the non-functional Green Fluorescent Protein (mGFP) gene carrying a TAG stop codon. These transgenic cells were bombarded with corrective oligonucleotide as editing reagent to recover GFP expression...
August 23, 2017: Journal of Plant Research
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