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https://www.readbyqxmd.com/read/28631163/evaluation-of-streck-bct-and-paxgene-stabilised-blood-collection-tubes-for-cell-free-circulating-dna-studies-in-plasma
#1
Kristina Warton, Nicole L Yuwono, Mark J Cowley, Mark J McCabe, Alwin So, Caroline E Ford
INTRODUCTION: Blood samples for studies of circulating DNA in disease are often collected in clinical settings where prompt processing of samples is not possible. In order to avoid problems associated with leukocyte lysis after prolonged blood storage, stabilised blood tubes have been developed containing preservatives that prevent cell lysis. We evaluated Streck BCT tubes and PAXgene ccfDNA tubes, as well as standard EDTA blood collection tubes, in terms of DNA yield and fragment size...
June 19, 2017: Molecular Diagnosis & Therapy
https://www.readbyqxmd.com/read/28630890/drd4-gene-polymorphisms-as-a-risk-factor-for-children-with-attention-deficit-hyperactivity-disorder-in-iranian-population
#2
Seyed Mahmoud Tabatabaei, Shahrokh Amiri, Sara Faghfouri, Seyed Gholamreza Noorazar, Shahin AbdollahiFakhim, Ali Fakhari
BACKGROUND AND OBJECTIVE: Dopamine dysfunction is known to be associated with attention deficit hyperactivity disorder (ADHD). Dopamine D4 receptor gene (DRD4) is one of the important genes in this pathway. This study intended to investigate the variable number of tandem repeats (VNTR) in exon 3 of the DRD4 gene in Iranian children and adolescents. MATERIALS AND METHODS: In this study, 130 children with ADHD, aged 6-14 years, and 130 healthy children, within the same age range, were enrolled...
2017: International Scholarly Research Notices
https://www.readbyqxmd.com/read/28627973/profile-of-gene-expression-of-tlr-signaling-pathways-in-colorectal-cancer-tissues
#3
Martyna Bednarczyk, Małgorzata Muc-Wierzgoń, Katarzyna Walkiewicz, Teresa Kokot, Edyta Fatyga, Urszula Mazurek
Toll-like receptors (TLRs) are involved in transduction of molecular signals in immune process such as induction and regulation of immunity, production of cytokines, and recognition of specific molecular patterns on the surface of microorganisms, but also in cancer development-which was partially proven in previous studies. There is a lack of detailed research on differentiating levels of TLR expression in colorectal cancer at different stages of its advancement, so in our study we want to determine whether there is such a difference of TLRs and TLR-connected protein expression...
June 1, 2017: International Journal of Immunopathology and Pharmacology
https://www.readbyqxmd.com/read/28627827/a-case-of-waldenstrom-macroglobulinemia-with-temporary-appearance-of-7s-igm-half-molecule
#4
Mayumi Imoto, Koji Yoshida, Yasuhiro Maeda, Ken-Ichi Nakae, Masatoshi Kudo, Ikunosuke Sakurabayashi, Toshiyuki Yamada, Toshinori Kamisako
BACKGROUND: We encountered a rare case of Waldenstrom macroglobulinemia with temporary appearance of 7S IgM half molecule and with monoclonal proteins binding to agarose gel. METHODS: The patient's serum and urine were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. The N-terminal amino acid sequences of the IgM with abnormal mass (68 kDa) were determined and compared with those of known immunoglobulin. RESULTS: The 68 kDa IgM consisted of a defective μ chain (36 kDa) and an intact κ chain...
May 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28623584/dnase-i-sim-a-simplified-in-nucleus-method-for-dnase-i-hypersensitive-site-sequencing
#5
Sergei A Filichkin, Molly Megraw
Identifying cis-regulatory elements is critical in understanding the direct and indirect interactions that occur within gene regulatory networks. Current approaches include DNase-seq, a technique that combines sensitivity to the nonspecific endonuclease DNase I with high-throughput sequencing to identify regions of regulatory DNA on a genome-wide scale. Yet, challenges still remain in processing recalcitrant tissues that have low DNA content. Here, we describe DNase I SIM (for Simplified In-nucleus Method), a protocol that simplifies and facilitates generation of DNase-seq libraries from plant tissues for high-resolution mapping of DNase I hypersensitive sites...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28620670/pesticide-vapor-sensing-using-an-aptamer-nanopore-and-agarose-gel-on-a-chip
#6
Satoshi Fujii, Aiko Nobukawa, Toshihisa Osaki, Yuya Morimoto, Koki Kamiya, Nobuo Misawa, Shoji Takeuchi
A pesticide vapor sensor was developed using an agarose gel-based chip containing a nanopore sensing system. Vaporized omethoate was detected by the absorption into the gel, the complex formation with a DNA aptamer, and its obstruction at the nanopore. This strategy is applicable to other vapors, expanding the versatility of nanopore sensors.
June 16, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28618874/von-willebrand-factor-multimeric-structure-and-functional-activity-in-patients-with-atrial-fibrillation-with-and-without-oral-anticoagulation
#7
Sandra Lopez-Castaneda, Ignacio Valencia-Hernández, Carlos Arean, Daniel Godínez-Hernández, Martha Eva Viveros-Sandoval
von Willebrand factor (vWF) is a multimeric glycoprotein present in blood plasma. It is synthesized in megakaryocytes and endothelial cells, secreted into circulation in the form of high-molecular-weight multimers (HMWMs), and cleaved into shorter, less active multimers by ADAMTS13. It is essential for platelet adhesion and aggregation. Previous studies have investigated the relationship between vWF levels and thromboembolic events with little regard to vWF multimeric structure. Patients with atrial fibrillation (AF) exhibit higher plasma vWF and lower ADAMTS13 levels...
January 1, 2017: Clinical and Applied Thrombosis/hemostasis
https://www.readbyqxmd.com/read/28618746/male-infertility-is-significantly-associated-with-multiple-deletions-in-an-8-7-kb-segment-of-sperm-mtdna-in-pakistan
#8
Irfan Afzal Mughal, Asma Irfan, Sarwat Jahan, Abdul Hameed
BACKGROUND/AIM: This study aimed to find a link between sperm mitochondrial DNA mutations and male infertility in Pakistan. MATERIALS AND METHODS: DNA from semen samples was extracted and amplified by PCR using 7.8-kb deletion-specific primers. The PCR products were separated on agarose gel, visualized under UV-illumination, and then photographed. The results were genotyped and the data were analyzed using SPSS. RESULTS: Deletion analysis of the 8...
June 12, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28613249/hypoxia-inducible-factor-2-alpha-and-prolinhydroxylase-2-polymorphisms-in-patients-with-acute-respiratory-distress-syndrome-ards
#9
Annika Dötsch, Lewin Eisele, Miriam Rabeling, Katharina Rump, Kai Walstein, Alexandra Bick, Linda Cox, Andrea Engler, Hagen S Bachmann, Karl-Heinz Jöckel, Michael Adamzik, Jürgen Peters, Simon T Schäfer
Hypoxia-inducible-factor-2α (HIF-2α) and HIF-2 degrading prolyl-hydroxylases (PHD) are key regulators of adaptive hypoxic responses i.e., in acute respiratory distress syndrome (ARDS). Specifically, functionally active genetic variants of HIF-2α (single nucleotide polymorphism (SNP) [ch2:46441523(hg18)]) and PHD2 (C/T; SNP rs516651 and T/C; SNP rs480902) are associated with improved adaptation to hypoxia i.e., in high-altitude residents. However, little is known about these SNPs' prevalence in Caucasians and impact on ARDS-outcome...
June 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28612188/direct-quantification-of-solute-diffusivity-in-agarose-and-articular-cartilage-using-correlation-spectroscopy
#10
Janty S Shoga, Brian T Graham, Liyun Wang, Christopher Price
Articular cartilage is an avascular tissue; diffusive transport is critical for its homeostasis. While numerous techniques have been used to quantify diffusivity within porous, hydrated tissues and tissue engineered constructs, these techniques have suffered from issues regarding invasiveness and spatial resolution. In the present study, we implemented and compared two separate correlation spectroscopy techniques, fluorescence correlation spectroscopy (FCS) and raster image correlation spectroscopy (RICS), for the direct, and minimally-invasive quantification of fluorescent solute diffusion in agarose and articular cartilage...
June 13, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28611596/ethidium-bromide-modifies-the-agarose-electrophoretic-mobility-of-cag%C3%A2-ctg-alternative-dna-structures-generated-by-pcr
#11
Mário Gomes-Pereira, Darren G Monckton
The abnormal expansion of unstable simple sequence DNA repeats can cause human disease through a variety of mechanisms, including gene loss-of-function, toxic gain-of-function of the encoded protein and toxicity of the repeat-containing RNA transcript. Disease-associated unstable DNA repeats display unusual biophysical properties, including the ability to adopt non-B-DNA structures. CAG•CTG trinucleotide sequences, in particular, have been most extensively studied and they can fold into slipped-stranded DNA structures, which have been proposed as mutation intermediates in repeat size expansion...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28610775/cytotoxic-effect-of-a-novel-naphthylchalcone-against-multiple-cancer-cells-focusing-on-hematologic-malignancies
#12
Mariana Franzoni Maioral, Camila do Nascimento Bodack, Natália Marceli Stefanes, Álisson Bigolin, Alessandra Mascarello, Louise Domeneghini Chiaradia-Delatorre, Rosendo Augusto Yunes, Ricardo José Nunes, Maria Cláudia Santos-Silva
Chalcones are natural compounds described in the literature by its several properties including cytotoxic activity against several tumor types. Considering that the search for new chemotherapeutic agents is still necessary, the aim of this study was to investigate the cytotoxic mechanisms involved in cell death induced by a synthetic chalcone (A23) on different tumor cells. Chalcone A23 reduced the cell viability of twelve tumor cell lines in a concentration and time dependent manner and it was more cytotoxic against acute leukemia cells...
June 10, 2017: Biochimie
https://www.readbyqxmd.com/read/28599438/androgen-deprivation-therapy-has-no-effect-on-pim-1-expression-in-a-mouse-model-of-prostate-cancer
#13
Jiang Wang, Gang Li, Bo Li, Hualin Song, Zhiqun Shang, Ning Jiang, Yuanjie Niu
The aim of the present study was to observe the dynamic changes of proto-oncogene, serine/threonine kinase, Pim-1 at the gene and protein level in a mouse model of prostate cancer following surgical castration. Using LNCaP cells to establish a subcutaneous xenograft model and orthotopic prostate cancer BALB/c nude mouse models, the xenograft models were divided into an androgen-dependent prostate cancer group (ADPC), an androgen deprivation therapy (ADT) group and an androgen independent prostate cancer (AIPC) group...
June 2017: Oncology Letters
https://www.readbyqxmd.com/read/28599057/dynamic-nuclear-polarization-studies-of-nitroxyl-spin-probes-in-agarose-gel-using-overhauser-enhanced-magnetic-resonance-imaging-omri
#14
V Meenakumari, Hideo Utsumi, Fuminori Hyodo, A Jawahar, A Milton Franklin Benial
Agarose is a tissue-equivalent material and its imaging characteristics similar to those of real tissues. Hence the dynamic nuclear polarization studies of carboxy-PROXYL in agarose gel were carried out. The DNP parameters such as spin lattice relaxation time, longitudinal relaxivity, leakage factor, saturation parameter and coupling parameter were estimated for 2 mM carboxy-PROXYL in PBS solution and water/agarose mixture (99:1). From these results, the spin probe concentration was optimized as 2 mM, and the reduction in enhancement was observed for carboxy-PROXYL in water/agarose mixture (99:1) compared with PBS solution...
June 9, 2017: Magnetic Resonance in Chemistry: MRC
https://www.readbyqxmd.com/read/28590243/detection-of-actinomyces-spp-in-cervical-exudates-from-women-with-cervical-intraepithelial-neoplasia-or-cervical-cancer
#15
Alejandra García-García, Jaime Coronel-Martínez, David Cantú-de Leon, María Del Socorro Romero-Figueroa, Yolanda Elizabeth Caballero-Pantoja, Gauddy Lizeth Manzanares-Leal, Miguel Rodriguez-Morales, Horacio Sandoval-Trujillo, Ninfa Ramírez-Durán
PURPOSE: Under certain circumstances, Actinomyces behaves as an opportunistic microorganism and can cause actinomycosis, a chronic and inflammatory granulomatous infection. The purpose of this project was to detect the presence of Actinomyces in cervical exudates from women with cervical intraepithelial neoplasia (CIN) and women with cervical cancer. METHODOLOGY: Cervical samples from 92 women were divided into three groups: CIN, cervical cancer and healthy women...
June 8, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28587967/thermal-stability-of-red-algal-galactans-effect-of-molecular-structure-and-counterions
#16
Marju Robal, Kalle Truus, Olga Volobujeva, Enn Mellikov, Rando Tuvikene
Thermal degradation of κ-, ι-, λ-carrageenans, furcellaran, funoran and agarose samples in dry and sol states was investigated. The polysaccharides subjected to heat treatment were characterized by (1)H NMR, (13)C NMR and FTIR spectroscopy, size exclusion chromatography and static rheometry methods. The microstructure of galactan gels was studied using a cryofixation method in combination with freeze-drying and SEM techniques. Thermal stability at high temperatures decreases in the order of agarose>furcellaran>funoran>κ-carrageenan>λ-carrageenan>ι-carrageenan for dry preparations...
June 3, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28585492/electrosensitization-increases-antitumor-effectiveness-of-nanosecond-pulsed-electric-fields-in-vivo
#17
Claudia Muratori, Andrei G Pakhomov, Loree Heller, Maura Casciola, Elena Gianulis, Sergey Grigoryev, Shu Xiao, O N Pakhomova
Nanosecond pulsed electric fields are emerging as a new modality for tissue and tumor ablation. We previously reported that cells exposed to pulsed electric fields develop hypersensitivity to subsequent pulsed electric field applications. This phenomenon, named electrosensitization, is evoked by splitting the pulsed electric field treatment in fractions (split-dose treatments) and causes in vitro a 2- to 3-fold increase in cytotoxicity. The aim of this study was to show the benefit of split-dose treatments for in vivo tumor ablation by nanosecond pulsed electric field...
January 1, 2017: Technology in Cancer Research & Treatment
https://www.readbyqxmd.com/read/28585379/groel-is-a-suitable-genetic-marker-for-detecting-vibrio-parahaemolyticus-by-loop-mediated-isothermal-amplification-lamp-assay
#18
Mahbubul Pratik Siddique, Won Je Jang, Jong Min Lee, Sun-Hee Ahn, Sharmin Suraiya, Chang-Hoon Kim, In-Soo Kong
A groEL gene-based loop-mediated isothermal amplification (LAMP) assay was developed to detect Vibrio parahaemolyticus in contaminated seafood and water. The assay was optimized and conducted at 63°C for 40 min using Bacillus stearothermophilus (Bst) DNA polymerase, large fragment. Amplification was analyzed via multiple detection methods, including opacity, formation of white precipitate, DNA intercalating dyes (ethidium bromide and SYBR Green I), metal ion-binding indicator dye, calcein, and 2% agarose gel electrophoresis...
June 5, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28583097/nramp1-and-vdr-gene-polymorphisms-in-susceptibility-to-pulmonary-tuberculosis-among-andhra-pradesh-population-in-india-a-case-control-study
#19
Rooth Vasantha Medapati, Sridevi Suvvari, Sudhakar Godi, Paddaiah Gangisetti
BACKGROUND: The aim of the present study was to evaluate the association of NRAMP1 -3'UTR, 274-CT,VDR- Fok1 VDR-Taq1 Polymorphisms with the risk of pulmonary tuberculosis. METHOD: A case -control study was conducted on Andhra Pradesh Population of India. Analysis of gene polymorphisms of NRAMP1 gene (3'UTR, 274CT) and VDR gene (Fok1 and Taq1) was done by using Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in Tuberculosis (TB) patients and healthy controls...
June 5, 2017: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/28577992/simultaneous-detection-of-4-prototypic-rat-parvoviruses-using-the-luminex-xtag-assay-in-laboratory-animal-health-monitoring
#20
Fengjiao Xu, Wen Yuan, Tongyuan Zhang, Yujun Zhu, Yuexiao Lian, Yu Zhang, Ren Huang, Pengju Guo
There are currently four rat parvoviruses including Kilham rat virus (KRV), Toolans H-1 parvovirus (H-1virus), rat parvovirus type 1a (RPV-1a) and rat minute virus (RMV). Virus detection methods are commonly based on conventional PCR - agarose gel electrophoresis or serological assay methods These methods are both time-consuming and lack specificity. In this study, we developed a bead array xTAG assay for the simultaneous detection and discrimination of four rat parvoviruses. The detection limits ranged from 100-1000 copies/μL of input purified plasmid DNA...
May 31, 2017: Journal of Virological Methods
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