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https://www.readbyqxmd.com/read/29166268/evaluation-of-usefulness-of-a-commercial-agarose-gel-electrophoresis-kit-quickgel-sp-for-bovine-serum-protein-electrophoresis
#1
Y Okatsu, N Yamagishi, K Hatate, B Devkota
The aim of this study was to show the usefulness of a commercial agarose gel electrophoresis (AGE) kit (QuickGel SP) for separating bovine serum protein fractions in comparison with conventional cellulose acetate electrophoresis (CAE). Serum protein bands were verified using five reference reagents corresponding to albumin and α1-, β1-, β2-, and γ-globulins. AGE clearly revealed six separated fractions of albumin and α1-, α2-, β1-, β2-, and γ-globulin fractions in 100% and 77.8% in serum samples of dairy cows from the healthy (n=27) and diseased groups (n=27), respectively...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29161491/dengue-virus-detection-in-aedes-aegypti-larvae-and-pupae-collected-in-rural-areas-of-anapoima-cundinamarca-colombia
#2
Myriam Lucía Velandia-Romero, Víctor Alberto Olano, Carolina Coronel-Ruiz, Laura Cabezas, María Angélica Calderón-Peláez, Jaime Eduardo Castellanos, María Inés Matiz
INTRODUCTION: There is a high incidence and prevalence of dengue in the department of Cundinamarca, and recently Aedes aegypti, the main vector of dengue virus (DENV), was detected in some of its rural areas. OBJECTIVE: To evaluate viral transovarial transmission in larvae and pupae collected in rural areas of the municipality of Anapoima, Cundinamarca. MATERIALS AND METHODS: Live larvae and pupae were collected from 53 homes and later they were taken to the laboratory in Anapoima, where they were classified, pooled and frozen...
March 29, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29158701/study-of-dna-damage-caused-by-dipyrone-in-presence-of-some-transition-metal-ions
#3
Bruna Corrêa Roriz, Horacio Dorigan Moya
The DNA damage in the presence of dipyrone (used as its sodium salt, NaDip) and some transition metal ions in an air saturated ([O2] ≈ 0.25 mM) non-buffered solution at T = (25.0 ± 0.5)°C was investigated by agarose gel electrophoresis. As metal ions Cu(2+), Fe(3+), Ni(2+) and Mn(3+) were selected and evaluated in the present study because of the important role they play in a biological system. pUC19 plasmid DNA damage-induced by NaDip (80-600 μM) was observed in the presence of 100 μM Cu(2+)...
November 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/29155749/immobilization-of-caenorhabditis-elegans-to-analyze-intracellular-transport-in-neurons
#4
Shinsuke Niwa
Axonal transport and intraflagellar transport (IFT) are essential for axon and cilia morphogenesis and function. Kinesin superfamily proteins and dynein are molecular motors that regulate anterograde and retrograde transport, respectively. These motors use microtubule networks as rails. Caenorhabditis elegans (C. elegans) is a powerful model organism to study axonal transport and IFT in vivo. Here, I describe a protocol to observe axonal transport and IFT in living C. elegans. Transported cargo can be visualized by tagging cargo proteins using fluorescent proteins such as green fluorescent protein (GFP)...
October 18, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29151339/a-novel-tool-for-estrogenic-activity-measurements-in-water-using-diffusion-gradients-in-thin-film-coupled-with-an-estrogen-bioassay
#5
Wei Guo, Kersten Van Langenhove, Michael S Denison, Willy F Baeyens, Marc Elskens, Yue Gao
A novel type of diffusive gradients in thin-film (DGT) was combined with a chemically activated luciferase gene expression bioassay (CALUX) to measure estrogens in aquatic systems. The performance of this novel method was assessed with 17β-estradiol (E2) as the model steroid hormone, XAD 18 resin gel as the binding phase in the DGT method and VM7Luc4E2 cells (formerly BG1Luc4E2) for the Estrogen Responsive Element (ERE)-CALUX bioassay. The measured effective diffusion coefficient of E2 in agarose diffusive gel was 4...
November 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29149837/assessment-of-genetic-diversity-in-vigna-unguiculata-l-walp-accessions-using-inter-simple-sequence-repeat-issr-and-start-codon-targeted-scot-polymorphic-markers
#6
David Okeh Igwe, Celestine Azubike Afiukwa, Benjamin Ewa Ubi, Kenneth Idika Ogbu, Omena Bernard Ojuederie, George Nkem Ude
BACKGROUND: Assessment of genetic diversity of Vigna unguiculata (L.) Walp (cowpea) accessions using informative molecular markers is imperative for their genetic improvement and conservation. Use of efficacious molecular markers to obtain the required knowledge of the genetic diversity within the local and regional germplasm collections can enhance the overall effectiveness of cowpea improvement programs, hence, the comparative assessment of Inter-simple sequence repeat (ISSR) and Start codon targeted (SCoT) markers in genetic diversity of V...
November 17, 2017: BMC Genetics
https://www.readbyqxmd.com/read/29134971/detection-of-dna-fragmentation-in-liver-of-goats-exposed-to-lead-poisoning-in-bagega-district-of-zamfara-state-nigeria
#7
A T Jubril, O A Fagbohun, A A Adekola
The ubiquitous presence of lead (Pb) and its release by anthropogenic activities has remained a majorenvironmental pollution risk to both humans and animals. Lead toxicity has been associated with different systemic toxicitiesand biochemical impacts (such as oxidative stress and DNA damaging effects) with dire health consequences. In Nigeria,the health problem associated with lead toxicity has been overwhelming in the Bagega District of Zamfara State whereartisanal gold mining has resulted in widespread environmental lead contamination...
June 30, 2017: Nigerian Journal of Physiological Sciences: Official Publication of the Physiological Society of Nigeria
https://www.readbyqxmd.com/read/29131996/development-and-application-of-the-diffusive-gradients-in-thin-films-technique-for-simultaneous-measurement-of-methcathinone-and-ephedrine-in-surface-river-water
#8
Yan Zhang, Tingting Zhang, Changsheng Guo, Song Hou, Zhendong Hua, Jiapei Lv, Yuan Zhang, Jian Xu
In this study, a passive sampling technique, diffusive gradients in thin films (DGT) was developed to simultaneously measure two drugs, methcathinone (MC) and ephedrine (EPH) in surface water. Four types of binding gels and four types of filter membranes were tested for the optimal configuration. XAD18 agarose binding gel and agarose diffusive gel, together with polyethersulfone filter membrane were used for measuring MC and EPH in the DGT device. 5% NH3 in acetonitrile was used as the elution solvent, with the elution efficiency for MC and EPH higher than 71%...
November 10, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29130341/topoisomerases-inhibitory-activities-and-dna-binding-properties-of-9-methoxycamptothecin-from-nothapodytes-nimmoniana-j-graham-mabberly
#9
Haiyan Wang, Mingzhang Ao, Weitu Liu, Yun Bai, Yuanmin Zhu, Longjiang Yu
We have reported previously that 9-methoxycamptothecin (MCPT) showed significant antitumor activity in vitro. Here, agarose gel electrophoresis experiments were performed to evaluate MCPT's unwinding ability toward plasmid DNA and inhibitory activities against topoisomerases (Topo) I and II. Binding properties of MCPT to calf thymus DNA (CT-DNA) were evaluated by UV-vis, melting temperature, fluorescence, circular dichroism methodologies and molecular docking technique. Results showed that MCPT at 100 μM inhibited Topo I activity, but had no effect on Topo II...
November 13, 2017: Natural Product Research
https://www.readbyqxmd.com/read/29126301/coagulation-testing-in-the-core-laboratory
#10
William E Winter, Sherri D Flax, Neil S Harris
Primary hemostasis begins with endothelial injury. VWF, produced by endothelial cells, binds to platelets and links them to subendothelial collagen. Platelet-derived ADP and thromboxane activate non-adhered platelets via their GPIIb/IIIa receptors, allowing these platelets to participate in platelet aggregation. Secondary hemostasis is initiated with the binding of factor VII to extravascular tissue factor (TF). Factors II, VII, IX and X are vitamin K-dependent factors. The role of vitamin K is to assist in the addition of gamma carboxylate groups to glutamic acids in the "GLA" domains of these factors...
November 8, 2017: Laboratory Medicine
https://www.readbyqxmd.com/read/29117135/zno-nanoparticles-protect-rna-from-degradation-better-than-dna
#11
Jayden McCall, Joshua J Smith, Kelsey N Marquardt, Katelin R Knight, Hunter Bane, Alice Barber, Robert K DeLong
Gene therapy and RNA delivery require a nanoparticle (NP) to stabilize these nucleic acids when administered in vivo. The presence of degradative hydrolytic enzymes within these environments limits the nucleic acids' pharmacologic activity. This study compared the effects of nanoscale ZnO and MgO in the protection afforded to DNA and RNA from degradation by DNase, serum or tumor homogenate. For double-stranded plasmid DNA degradation by DNase, our results suggest that the presence of MgO NP can protect DNA from DNase digestion at an elevated temperature (65 °C), a biochemical activity not present in ZnO NP-containing samples at any temperature...
November 8, 2017: Nanomaterials
https://www.readbyqxmd.com/read/29116152/fabrication-of-artificial-leaf-to-develop-fluid-pump-driven-by-surface-tension-and-evaporation
#12
Minki Lee, Hosub Lim, Jinkee Lee
Plants transport water from roots to leaves via xylem through transpiration, which is an evaporation process that occurs at the leaves. During transpiration, suction pressure is generated by the porous structure of mesophyll cells in the leaves. Here, we fabricate artificial leaf consisting of micro and nano hierarchy structures similar to the mesophyll cells and veins of a leaf using cryo-gel method. We show that the microchannels in agarose gel greatly decrease the flow resistance in dye diffusion and permeability experiments...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29115844/scalable-fabrication-framework-of-implantable-ultrathin-and-flexible-probes-with-biodegradable-sacrificial-layers
#13
Xiangbing Jiao, Yuan Wang, Quan Qing
For long-term biocompatibility and performance, implanted probes need to further reduce their size and mechanical stiffness to match that of the surrounding cells, which, however, makes accurate and minimally invasive insertion operations difficult due to lack of rigidity and brings additional complications in assembling and surgery. Here, we report a scalable fabrication framework of implantable probes utilizing biodegradable sacrificial layers to address this challenge. Briefly, the integrated biodegradable sacrificial layer can dissolve in physiological fluids shortly after implantation, which allows the in situ formation of functional ultrathin film structures off of the initial small and rigid supporting backbone...
November 15, 2017: Nano Letters
https://www.readbyqxmd.com/read/29111376/analysis-of-the-proficiency-of-single-radial-immunodiffusion-assays-for-quality-control-of-influenza-vaccines-in-korea
#14
Yejin Choi, Sunmi Lee, Seong Yi Kwon, Younghee Lee, Yong Keun Park, Sang Ja Ban
Influenza vaccine potency, which is determined by quantitatively measuring the content of Hemagglutinin (HA), is an essential index representing the efficacy of the vaccine. Standardization of the single radial immunodiffusion (SRID) assay, a method for measuring HA content, and proficiency of the testing institutions are crucial for influenza vaccine quality control. Herein, we assessed the proficiency of SRID assays at the National Control Laboratory (NCL) of Korea and several vaccine manufacturers. Eight laboratories participated in this study, and the proficiencies of all laboratories yielded satisfactory results in overall SRID assays...
October 27, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/29108575/agarose-hydrogel-containing-immobilized-ph-buffer-microemulsion-without-increasing-permselectivity
#15
Miguel Coll Crespi, Gaston A Crespo, Xiaojiang Xie, Romain Touilloux, Marylou Tercier-Waeber, Eric Bakker
A heterogeneous pH buffer based on a colloidal emulsion containing ion-exchanger and lipophilic base is described that can be integrated into hydrogels without affecting their ion-exchange properties. Each sphere works on the basis of reversible ion-exchange of hydrogen ions with solution cations, acting as a pH buffer while staying removed from solution in the nonpolar core of the spheres. The ion-exchange mechanism is supported by titration experiments in aqueous emulsion, showing that the nature and concentration of the exchanging solution cations influences the buffer action, with increasing lipophilicity moving the equilibrium to lower pH values...
January 15, 2018: Talanta
https://www.readbyqxmd.com/read/29097916/a-method-for-manufacturing-oncological-phantoms-for-the-quantification-of-18f-fdg-pet-and-dw-mri-studies
#16
Francesca Gallivanone, Irene Carne, Matteo Interlenghi, Daniela D'Ambrosio, Maurizia Baldi, Daniele Fantinato, Isabella Castiglioni
The aim of this work was to develop a method to manufacture oncological phantoms for quantitation purposes in 18F-FDG PET and DW-MRI studies. Radioactive and diffusion materials were prepared using a mixture of agarose and sucrose radioactive gels. T2 relaxation and diffusion properties of gels at different sucrose concentrations were evaluated. Realistic oncological lesions were created using 3D-printed plastic molds filled with the gel mixture. Once solidified, gels were extracted from molds and immersed in a low-radioactivity gel simulating normal background tissue...
2017: Contrast Media & Molecular Imaging
https://www.readbyqxmd.com/read/29089077/-prokaryotic-expression-and-purification-of-recombinant-rat-hmgb1-a
#17
Linquan He, Jiayun Liu, Yuanli Qu, Xitao Liu, Xiaodong Li, Yin Long
Objective To obtain the recombinant A-domain of high mobility group box 1 (HMGB1-A) protein. Methods Using genetic engineering techniques, we cloned and recombined rat HMGB1-A gene. Using prokaryotic expression technique, we expressed and purified the recombinant rat HMGB1-A protein, which was verified by Western blotting. Results Agarose gel electrophoresis analysis showed that HMGB1-A cassette gene size was about 250 bp; double digestion for identifying the recombinant clone pUC-A showed that the product size was about 3000 bp, 250 bp; PCR for identifying the recombinant clone pGEX-A showed that the product size was about 250 bp...
September 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/29083312/bioprinted-chitosan-gelatin-thermosensitive-hydrogels-using-an-inexpensive-3d-printer
#18
Kevin D Roehm, Sundararajan V Madihally
The primary bottleneck in bioprinting cell-laden structures with carefully controlled spatial relation is a lack of biocompatible inks and printing conditions. In this regard, we explored using thermogelling chitosan-gelatin (CG) hydrogel as a novel bioprinting ink; CG hydrogels is unique in that it undergoes a spontaneous phase change at physiological temperature, and does not need post-processing. In addition, we used a low cost (<$800) compact 3D printer, and modified with a new extruder to print using disposable syringes and hypodermic needles...
October 30, 2017: Biofabrication
https://www.readbyqxmd.com/read/29079358/monoclonal-protein-reference-change-value-as-determined-by-gel-based-serum-protein-electrophoresis
#19
Mina Salamatmanesh, Christopher R McCudden, Arleigh McCurdy, Ronald A Booth
INTRODUCTION: The International Myeloma Working Group recommendations for monitoring disease progression or response include quantitation of the involved monoclonal immunoglobulin. They have defined the minimum change criteria of ≧25% with an absolute change of no <5g/L for either minimal response or progression. Limited evidence is available to accurately determine the magnitude of change in a monoclonal protein to reflect a true change in clinical status. Here we determined the analytical and biological variability of monoclonal proteins in stable monoclonal gammopathy of undetermined significance (MGUS) patients...
October 24, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/29074292/subarachnoid-hemorrhage-induces-neuronal-nitric-oxide-synthase-phosphorylation-at-ser-1412-in-the-dentate-gyrus-of-the-rat-brain
#20
Kentaro Wada, Koji Osuka, Yasuo Watanabe, Nobuteru Usuda, Motoaki Fukasawa, Yoshio Araki, Sho Okamoto, Toshihiko Wakabayashi
INTRODUCTION: We previously demonstrated that cyclic AMP-dependent protein kinase (PKA) phosphorylates neuronal nitric oxide synthase (nNOS) at Ser(1412) in the hippocampal dentate gyrus after forebrain ischemia; this phosphorylation event activates NOS activity and might contribute to depression after cerebral ischemia. In this study, we revealed chronological and topographical changes in the phosphorylation of nNOS at Ser(1412) immediately after subarachnoid hemorrhage (SAH). METHODS: In a rat single-hemorrhage model of SAH, the hippocampus and adjacent cortex were collected up to 24 h after SAH...
October 23, 2017: Nitric Oxide: Biology and Chemistry
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