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Agarose gel

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https://www.readbyqxmd.com/read/29792547/a-method-to-quantify-genotoxicity-of-malathion-in-rainbow-trout-using-the-weighted-averaging
#1
Hadi Poorbagher, Hamed Ghaffari Farsani, Hamid Farahmand
DNA breakage has been frequently used as a biomarker of the pesticide toxicity. The present study introduced a method to quantify the DNA breakage in Oncorhynchus mykiss exposed to the pesticide Malathion. Specimens were exposed to different concentrations of malathion for 1-9 days and their gill and liver were sampled. DNA was extracted and electrophoresed using agarose gel. The pixel density curves were obtained from the gel smears. The area under the curves were arbitrarily divided from 3 up to 7 segments using a Java macro in the software ImageJ...
May 24, 2018: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/29786746/luteolin-induces-myelodysplastic-syndrome%C3%A2-derived-cell-apoptosis-via-the-p53%C3%A2-dependent-mitochondrial-signaling-pathway-mediated-by-reactive-oxygen-species
#2
Weimin Dong, Yan Lin, Yang Cao, Yue Liu, Xiaobao Xie, Weiying Gu
Luteolin, a common dietary flavonoid, induces the apoptosis of cells in several types of cancer. However, its role in myelodysplastic syndrome (MDS) and the potential underlying mechanisms remain to be elucidated. To evaluate the potential benefit and underlying mechanisms of luteolin in MDS cells, the viability of SKM‑1 cells and primary bone marrow (PBM) mononuclear cells from patients with intermediate‑ or high‑risk MDS were assessed using a Cell Counting Kit‑8 assay. The apoptotic features of cell morphology were assessed using Wright‑Giemsa staining, DNA fragmentation was analyzed by agarose gel electrophoresis, and the extent of apoptosis was quantified by flow cytometry (FCM)...
May 18, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29784272/design-and-synthesis-of-new-antitumor-agents-with-the-1-7-epoxycyclononane-framework-study-of-their-anticancer-action-mechanism-by-a-model-compound
#3
Ángel M Montaña, Julia Lorenzo, Stefano Ponzano, Maria-Filomena Sanasi
This article describes the design, synthesis and biological evaluation of a new family of antitumor agents having the 1,7-epoxycyclononane framework. We have developed a versatile synthetic methodology that allows the preparation of a chemical library with structural diversity and in good yield. The synthetic methodology has been scaled up to the multigram level and can be developed in an enantioselective fashion. The study in vitro of a model compound, in front of the cancer cell lines HL-60 and MCF-7, showed a growth inhibitory effect better than that of cisplatin...
May 18, 2018: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29781218/fabrication-of-single-gel-with-different-mechanical-stiffness-using-three-dimensional-mold
#4
Gulsan Ara Sathi Kazi, Kazi Anisur Rahman, Mahmoud Farahat, Takuya Matsumoto
The hydrogel is considering as functional substrates for three-dimensional (3D) environment mimicking the native tissue in vitro. To get the cell or tissue culture result in different stiffness, researchers are used separate gel at the different time. Sometimes these results are manipulated by surrounding environment. To overcome this, we prepared a single hydrogel with different young modulus using a 3D printed mold and cell culture and tissue culture was performed to check the functional capacity. In this proposed device we successfully produced a multi properties agarose hydrogel on a single platform...
May 20, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29776310/preparation-and-evaluation-of-ribonuclease-resistant-viral-hiv-rna-standards-based-on-armored-rna-technology
#5
Mohammad Gholami, Mehrdad Ravanshad, Kazem Baesi, Siamak M. Samiee, Negin Hosseini Rozbahani, Minoo Mohraz
BAckground: The human immunodeficiency virus type 1 (HIV-1) is an infectious viral agent that gradually extinguishes the immune system, resulting in acquired immune deficiency syndrome (AIDS). The aim of this study was to construct an RNA-positive control based on armored (AR) RNA technology, using HIV-1 RNA as a model. Methods: The MS2 maturase, a coat protein gene (at positions 1765 to 1787) and HIV-1 pol gene were cloned into pET-32a plasmid. The prepared plasmid was transformed into Escherichia coli strain BL2 (DE3), and the expression of the construct was induced by 1 mM of isopropyl-L-thio-D-galactopyranoside (IPTG) at 37 °C for 16 h to obtain the fabricated AR RNA...
May 19, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29771344/prevalence-of-rickettsia-species-rickettsiales-rickettsiaceae-in-dermacentor-variabilis-ticks-acari-ixodidae-in-north-carolina
#6
Madhavi L Kakumanu, Loganathan Ponnusamy, Haley Sutton, Steven R Meshnick, William L Nicholson, Charles S Apperson
The American dog tick, Dermacentor variabilis (Say), is a vector of spotted fever group (SFG) rickettsiae, including Rickettsia rickettsii the causative organism of Rocky Mountain spotted fever (RMSF). In North Carolina, SFG rickettsioses (including RMSF) are a leading cause of tick-borne illness. Knowledge of the infection rate and geographic distribution of D. variabilis ticks infected with Rickettsia spp. provides information on the spatial distribution of public health risk. Accordingly, we extracted genomic DNA from adult D...
May 16, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29771238/on-the-direct-acquisition-of-beam-s-eye-view-images-in-mri-for-integration-with-external-beam-radiotherapy
#7
Keith Wachowicz, Brad Murray, B Gino Fallone
The recent interest in the integration of external beam radiotherapy with a magnetic resonance (MR) imaging unit offers the potential for real-time adaptive tumour tracking during radiation treatment. The tracking of large tumours which follow a rapid trajectory may best be served by the generation of a projection image from the perspective of the beam source, or "beam's eye view" (BEV). This type of image projection represents the path of the radiation beam, thus enabling rapid compensations for target translations, rotations and deformations, as well time-dependent critical structure avoidance...
May 17, 2018: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/29768722/genoprotective-effect-of-phyllanthus-orbicularis-extract-against-uva-uvb-and-solar-radiation
#8
Marioly Vernhes Tamayo, André Passaglia Schuch, Teiti Yagura, Luis Baly Gil, Carlos Frederico Martins Menck, Angel Sánchez-Lamar
One approach to protect the human skin against harmful effects of solar ultraviolet (UV) radiation is to use natural products as photoprotectors. In this work, the extract from specie Phyllanthus orbicularis K was evaluated as a protective agent against the photodamage by UVB, UVA artificial lamps and environmental sunlight exposure. The plasmid DNA solutions were exposed to radiations using the DNA-dosimeter system in presence of plant extract. The DNA repair enzymes, E. coli Formamidopyrimidine-DNA glycosylase (Fpg) and T4 bacteriophage endonuclease V (T4-endo V), were employed to discriminate oxidized DNA damage and cyclobutane pyrimidine dimers (CPD) respectively...
May 16, 2018: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/29768691/detection-of-hepatitis-b-virus-dna-among-chronic-and-potential-occult-hbv-patients-in-resource-limited-settings-by-loop-mediated-isothermal-amplification-assay
#9
Arifa Akram, S M Rashedul Islam, Saif Ullah Munshi, Shahina Tabassum
Transmission of Hepatitis B Virus (HBV) usually occurs due to the transfusion of blood or blood products from chronic HBV (CHB) or occult HBV infected (OBI) patients. Besides serological tests e.g. HBsAg and anti-HBc (total), detection of HBV-DNA is necessary for the diagnosis of OBI patients. Different nucleic acid tests (NATs) including real-time-Polymerase Chain Reaction (qPCR) are used for the detect HBV-DNA. The NATs are expensive and require technical expertise which are barriers to introducing them in resource-limited settings...
May 16, 2018: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/29761414/spatially-resolved-kinetics-of-skeletal-muscle-exercise-response-and-recovery-with-multiple-echo-diffusion-tensor-imaging-mediti-a-feasibility-study
#10
E E Sigmund, S H Baete, K Patel, D Wang, D Stoffel, R Otazo, P Parasoglou, J Bencardino
OBJECTIVES: We describe measurement of skeletal muscle kinetics with multiple echo diffusion tensor imaging (MEDITI). This approach allows characterization of the microstructural dynamics in healthy and pathologic muscle. MATERIALS AND METHODS: In a Siemens 3-T Skyra scanner, MEDITI was used to collect dynamic DTI with a combination of rapid diffusion encoding, radial imaging, and compressed sensing reconstruction in a multi-compartment agarose gel rotation phantom and within in vivo calf muscle...
May 14, 2018: Magma
https://www.readbyqxmd.com/read/29757289/use-of-two-dimensional-semi-denaturing-detergent-agarose-gel-electrophoresis-to-confirm-size-heterogeneity-of-amyloid-or-amyloid-like-fibers
#11
Sarah Hanna-Addams, Zhigao Wang
Amyloid or amyloid-like fibers have been associated with many human diseases, and are now being discovered to be important for many signaling pathways. The ability to readily detect the formation of these fibers under various experimental conditions is essential for understanding their potential function. Many methods have been used to detect the fibers, but not without some drawbacks. For example, electron microscopy (EM), or staining with Congo Red or Thioflavin T often requires purification of the fibers...
April 26, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29755768/detecting-promoter-methylation-pattern-of-apoptotic-genes-apaf1-and-caspase8-in-gastric-carcinoma-patients-undergoing-chemotherapy
#12
Fatemeh Azarkhazin, Golnaz Asaadi Tehrani
Background: DNA methylation patterns in cells dysregulation CpG island methylation of genes involved in cancer leads to increased levels of the cancer. Restoration of the apoptotic route in tumor cells of stomach in order for placing Casp8 and Apaf1 genes is a proper approach for new treatments of gastric cancer. The objective of the present study was to investigate the relationship between the pattern of methylation promoter in apoptotic genes of Casp8 and Apaf1 and gastric carcinoma in patients receiving chemotherapy...
April 2018: Journal of Gastrointestinal Oncology
https://www.readbyqxmd.com/read/29753951/preparation-of-well-defined-brush-like-block-copolymers-for-gene-delivery-applications-under-biorelevant-reaction-conditions
#13
Joana R Góis, Fábio Reis, Ana M Almeida, Patrícia Pereira, Fani Sousa, Arménio C Serra, Jorge F J Coelho
Well-defined oligo(ethylene glycol) methyl ether methacrylate (OEOMA) based block copolymers with cationic segments composed by N,N-(dimethylamino) ethyl methacrylate (DMAEMA) and/or 2-(diisopropylamino) ethyl methacrylate (DPA) were developed under biorelevant reaction conditions. These brush-type copolymers were synthesized through supplemental activator and reducing agent (SARA) atom transfer radical polymerization (ATRP) using sodium dithionite as SARA agent. The synthesis was carried out using an eco-friendly solvent mixture, very low copper catalyst concentration, and mild reaction conditions...
May 3, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29751036/guar-gum-blended-alginate-agarose-hydrogel-as-a-promising-support-for-the-entrapment-of-peroxidase-stability-and-reusability-studies-for-the-treatment-of-textile-effluent
#14
Misha Ali, Qayyum Husain
Ginger peroxidase (GP) was entrapped into the hydrogels of guar gum (GG)-alginate/agarose and these immobilized GP preparations were employed for the treatment of textile effluent. GG is a natural hydrophilic polysaccharide which may form complex with enzyme increasing its molecular dimension that can be easily retained inside the entrapping support. Therefore, the activity retention by alginate-guar gum (ANGG) and agarose-guar gum (AGG) was higher than that of alginate and agarose alone. Scanning electron microscopy (SEM) analysis also revealed that GG-GP complex was successfully entrapped inside the alginate/agarose gels...
May 8, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29748810/analyzing-the-uncertainty-of-diffusive-gel-based-passive-samplers-as-tools-for-evaluating-the-averaged-contamination-of-surface-water-by-organic-pollutants
#15
Angel Belles, Claire Alary, Nellaïdeve Laguerre, Christine Franke
Agarose gel-based passive samplers are nowadays one of the most effective sampling devices able to provide a quantitative evaluation of water contamination level for a broad range of organic contaminants. These devices show significant improvements in comparison to the previous passive samplers dedicated to hydrophilic compounds because they tend to reduce the effect of hydrodynamic flow conditions on the uptake rate of compounds and thus subsequently to improve their accuracy. However, albeit their effects minimized, hydrodynamic water flow and temperature are reported as variables likely to change the uptake rate of compounds that may lead to some inaccuracy if they are not adequately taken into account...
May 11, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29747479/synthesis-and-biological-evaluation-of-bisthiazoles-and-polythiazoles
#16
Mohammed A Al-Omair, Abdelwahed R Sayed, Magdy M Youssef
Small heterocyclic compounds containing nitrogen and sulfur atoms, such as thiazole derivatives, represent a significant class of organic azoles that exhibit promising bioactivities and have a great potential in medicinal and agricultural fields. A convenient and high-yielding synthetic approach for a range of organic molecules is presented. The nuclease-like activities of compounds were studied with the aid of E. coli AB1157 DNA and agarose gel electrophoresis. The antioxidant evaluation of the compounds was carried out with different antioxidant techniques, such as ABTS and NO scavenging efficiency...
May 10, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29746616/a-regulatory-insertion-deletion-polymorphism-in-the-fads-gene-cluster-influences-pufa-and-lipid-profiles-among-chinese-adults-a-population-based-study
#17
Peiqin Li, Jing Zhao, Kumar S D Kothapalli, Xiang Li, Hui Li, Yuxuan Han, Shengquan Mi, Wenhua Zhao, Qizhai Li, Hong Zhang, Yiqing Song, J Thomas Brenna, Ying Gao
Background: Arachidonic acid (AA) is the major polyunsaturated fatty acid (PUFA) substrate for potent eicosanoid signaling to modulate inflammation and thrombosis and is controlled in part by tissue abundance. Fatty acid desaturase 1 (FADS1) catalyzes synthesis of omega-6 (n-3) AA and n-3 eicosapentaenoic acid (EPA). The rs66698963 polymorphism, a 22-base pair (bp) insertion-deletion 137 bp downstream of a sterol regulatory element in FADS2 intron 1, mediates expression of FADS1 in vitro, as well as exerting positive selection in several human populations...
May 9, 2018: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/29745740/co-delivery-of-gambogenic-acid-and-vegf-sirna-with-anionic-liposome-and-polyethylenimine-complexes-to-hepg2-cells
#18
Qiongfang Yu, Bian Zhang, Yali Zhou, Qin Ge, Chang Jiali, Yunna Chen, Kaiqi Zhang, Daiyin Peng, Weidong Chen
Background and Objective The combination of two or more different mechanisms of drugs in the treatment of cancer has become one of the effective methods. The purpose of this study was to successfully prepare a non-viral delivery system that could efficiently co-delivery siRNA and gambogenic acid (GNA) to improve the anti-cancer efficiency in HepG2 cells. Methods The delivery system was prepared by a two-step method. Firstly, the GNA anionic liposome took shape by a solvent evaporation method, and then the liposome was bound to the PEI/siRNA complex by electrostatic interaction to form the final carrier system (lipopolyplexes)...
May 10, 2018: Journal of Liposome Research
https://www.readbyqxmd.com/read/29739120/concordance-of-capillary-electrophoresis-and-conventional-gel-electrophoresis-in-two-different-groups-of-patients-with
#19
Suekyeung Kim, Hyung-Seok Yang, Anbok Lee, Sun Young Cho
BACKGROUND: Serum and urinary protein electrophoresis play an important role in the identification of monoclonal gammopathy. Recently, capillary electrophoresis (CE) has been adapted in many clinical laboratories because of several advantages such as short turnaround time, automation, and high reproducibility. However, there have been unsolved concerns for the concordance between conventional gel and automated capillary electrophoresis methods for protein separation in clinical specimens...
March 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/29736383/analysis-of-kras-gene-from-induced-pancreatic-cancer-rats-administered-with-momordica-charantia-and-ocimum-ba-s-ilicum-leaf-extracts
#20
J B Minari, C A Okelola, N C Ugochukwu
Objective: To analyze K-ras gene from induced pancreatic cancer rats administered with Momordica charantia and Ocimum basilicum leaf extracts. Methods: Twenty-five (25) adult rats weighing between 90-120 g were divided into 5 groups namely RA, RB, RC, NC and PC, each group had 5 rats. The PC which served as the control was fed with normal fish meal and water ad libitum; the NC which is the negative control received 20 mg/ml/week of Nitrosamines only while other groups received different concentrations of aqueous extract of both M ...
April 2018: Journal of Traditional and Complementary Medicine
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