keyword
https://read.qxmd.com/read/38527285/support-for-research-biobanks-among-medical-students-in-the-republic-of-kazakhstan
#1
JOURNAL ARTICLE
Dariusz Walkowiak, Alua Miraleyeva, Tamara Batyrova, Jan Domaradzki
Background: Over the past few years, several biobanks have been organized in the Republic of Kazakhstan (RoK). As research biobanks are becoming an increasingly important tool for precision medicine, they require competent biobankers who will help disseminate the idea of biobanking throughout the society and support donation for research purposes. This study aimed to assess the support for research biobanks among medical students (MSs) in Kazakhstan. Methods: This research includes data from an anonymous, self-administered online questionnaire regarding MSs' support for research biobanks in the RoK...
March 25, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38527284/effect-of-moderate-static-magnetic-fields-on-mice-oocyte-vitrification-calcium-related-genes-expression
#2
JOURNAL ARTICLE
Sara Soleimani Pargoo, Farzaneh Baniasadi, Vida Sadat Kazemein Jasemi, Samira Hajiaghalou, Mohsen Gharanfoli, Rouhollah Fathi
The ability to cryopreserve oocytes without ultrastructural injury has been a concern in the development and use of methods to preserve female reproduction. The stability of the cell membrane must be preserved to reduce the damage caused by ice crystals during vitrification. One approach that has been explored is the use of static magnetic fields (SMFs), which are believed to influence cell membrane stability. In this study, the in vitro effects of SMF that range between 20-80 mT on the vitrification of mice germinal vesicle (GV) oocytes were studied...
March 25, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38526566/evaluation-of-collection-and-processing-conditions-for-gene-expression-analysis-using-human-myeloid-cells
#3
JOURNAL ARTICLE
Hitoshi Miyashita, Issey Takehara, Masatoshi Nishimura, Gensuke Takayama, Hiroyuki Sumi, Michinori Kadokura, Daisuke Nakai
Background: The population of blast cells among peripheral blood mononuclear cells (PBMCs) obtained from patients is a desirable specimen for analyzing gene expression in diseases including acute myeloid leukemia. Although the enrichment of blast cells often needs to be performed at a central laboratory, acceptable conditions for sample transport from clinical sites remain to be established. Methods: We evaluated storage temperature, duration, and tube type before initiating sample processing for the analysis of cluster of differentiation (CD)33+ myeloid cells among PBMCs as an alternative to CD34+ /CD33+ blast cells...
March 22, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38526565/catalase-and-uric-acid-prevent-morphological-damage-to-the-sperm-flagella-of-colossoma-macropomum-during-96-hours-at-low-storage-temperatures
#4
JOURNAL ARTICLE
Yugo M Pastrana, Jaydione L Marcon, Amanda P de Amaral, Francisco Bruno P Santos, Emerson S Lima, Leonard D R Acho, Rodrigo Otávio S de Souza, Carolina C Grando, Danilo P Streit Junior, Leandro Godoy
Oxidative stress is one of the main causes of loss of sperm function during chilled storage. The aim of the current study was to evaluate the effects of a fructose-based extender, which was supplemented with catalase or uric acid, on the motility, viability, morphological integrity, and lipid peroxidation (LPO) of Colossoma macropomum spermatozoa. Sperm was diluted in extenders containing catalase (0; 0.1; 0.8; and 1.5 kU/L) or uric acid (0; 0.25; 0.5; and 1.0 mmol/L) and then stored at 4.3 ± 0...
March 22, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38497765/update-of-the-minimum-information-about-biobank-data-sharing-miabis-core-terminology-to-the-3-rd-version
#5
JOURNAL ARTICLE
Niina Eklund, Cäcilia Engels, Michael Neumann, Andrzej Strug, Esther van Enckevort, Ronny Baber, Margreet Bloemers, Annelies Debucquoy, Aad van der Lugt, Heimo Müller, Lauri Parkkonen, Philip R Quinlan, Esmond Urwin, Petr Holub, Kaisa Silander, Gabriele Anton
Introduction: The Minimum Information About BIobank Data Sharing (MIABIS) is a biobank-specific terminology enabling the sharing of biobank-related data for different purposes across a wide range of database implementations. After 4 years in use and with the first version of the individual-level MIABIS component Sample , Sample donor, and Event , it was necessary to revise the terminology, especially to include biobanks that work more in the data domain than with samples. Materials & Methods: Nine use-cases representing different types of biobanks, studies, and networks participated in the development work...
March 18, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38497547/technology-for-biobanking-of-epididymal-spermatozoa-from-patient-with-obstructive-azoospermia-case-report-about-baby-born-after-conventional-freezing-only-with-a-nonpermeable-cryoprotectant-360-kda-polyvinylpyrrolidone
#6
JOURNAL ARTICLE
Maryna Petrushko, Taisiia Yurchuk, Volodymyr Piniaiev, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, Volodimir Isachenko
This publication reports, for the first time, the birth of a healthy child after intracytoplasmic sperm injection (ICSI) of motile spermatozoa after conventional ("slow") freezing of epididymal spermatozoa using 5% polyvinylpyrrolidone (PVP) of high molecular weight (360 kDa). Cryopreservation solution with 10% PVP was added to 30 µL of spermatozoa suspension in a 1:1 ratio, with a final PVP concentration of 5%. Then, polycarbonate capillaries for oocyte denudation with a diameter of 170 µm were filled with 60 µL of the resulting sperm suspension...
March 18, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38484300/visualization-of-ice-crystal-behavior-in-mouse-oocytes-during-high-speed-quench-cooling-and-ice-inhibition-by-antifreezing-hydrogels
#7
JOURNAL ARTICLE
Xin Li, Shuyong Zhang, Yuqi Zhang, Xinli Zhou
Oocyte vitrification has become a widely adopted method in clinical practice. However, the solidification behavior and its impact on oocytes during the ultrarapid cooling process remain poorly understood. In this study, we established a system and methodology to observe crystallization behavior in oocytes during quench cooling and warming. Subsequently, the threshold concentration of cryoprotective agents (CPAs) required for oocyte vitrification was determined through a visualization method. The results demonstrated that the ice front could not be observed in the image sequence when using 16...
March 14, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38457650/a-survey-of-community-perceptions-on-brain-donation-for-research
#8
JOURNAL ARTICLE
Alicia Sweeney, Amanda Rush, Julia Stevens, Greg T Sutherland
Postmortem brain donation for medical research is a little-known form of organ donation. While most brain research is carried out using animal models, many neurological diseases are uniquely human. Greater availability of human postmortem brain tissue from diseased individuals and controls would therefore improve the development of treatments for neurological and neuropsychiatric diseases. Globally, organ donation for medical research is dwarfed by organ donation for transplantation. In 2021, 36% of Australians were registered organ donors for transplantation, with public "in-principle" support even higher, at 76%...
March 8, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38452333/proteomic-analysis-of-preeclampsia-amniotic-fluid-based-on-a-novel-solid-state-preservation-method
#9
JOURNAL ARTICLE
Meiling Ge, Mengru Wang, Yanhong Liu, Hu Yue, Jie Ding, Xiaowei Wang, Tianlin Yao, Hong Gao
Objective: Amniotic fluid (AF) plays a crucial role in diagnosing and predicting perinatal diseases, specifically preeclampsia (PE). Adequate preservation of AF samples is essential for advancing the development of PE-related biomarkers and understanding the disease's mechanisms. Materials and Methods: This study presents a method for preserving proteins in AF on a solid medium, specifically a nitrocellulose membrane, which is referred to as an AF membrane. Samples were collected from normotensive subjects and PE patients and treated with direct freezing and the AF membrane methods, respectively...
March 7, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38452159/attenuation-of-oxidative-stress-in-erythrocytes-stored-with-vitamin-c-and-l-carnitine-in-additive-solution-7
#10
JOURNAL ARTICLE
Pallavi Masannagari, Vani Rajashekaraiah
Background: Blood transfusion has advanced toward component therapy for specific requirements during trauma and surgery. Oxidative stress is induced in erythrocytes during storage. Hence, antioxidants as additives can be employed to counteract oxidative stress and enhance antioxidant defenses. Therefore, this study investigates the combinatorial effects of vitamin C and l-carnitine on erythrocytes during storage. Methodology: Erythrocyte samples were categorized into control and experimental groups-vitamin C (10 mM) and l-carnitine (10 mM) and stored under blood bank conditions (at 4°C) for 35 days...
March 7, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38452158/effect-of-trehalose-supplementation-in-egg-yolk-free-extender-on-conventional-parameters-and-gene-expression-related-to-reactive-oxygen-species-apoptosis-and-motility-of-frozen-dog-spermatozoa
#11
JOURNAL ARTICLE
Saddah Ibrahim, Sangmin Shin, Nabeel Abdelbagi Hamad Talha, Yubyeol Jeon, Il-Jeoung Yu
The present study was conducted to evaluate the effects of trehalose supplementation in egg-yolk (EY)-free tris extender on dog spermatozoa. Pooled spermatozoa were diluted with extender 1 (EY-free tris extender supplemented with 0, 10, 15, 20, or 30 mM trehalose) and cooled (2 × 108 sperm/mL) for 1 hour at 4°C. After that, extender 2 (extender 1 containing 1 M glycerol) was added (v:v) to the diluted sperm, loaded in 0.5-mL straws (1 × 108 sperm/mL), and incubated at 4°C for 30 minutes...
March 7, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38416864/cell-free-microbial-dna-analysis-effects-of-blood-plasma-and-serum-quantity-biobanking-protocols-and-isolation-kits
#12
JOURNAL ARTICLE
Darja Nikitina, Rokas Lukosevicius, Deimante Tilinde, Tomas Muskieta, Johannes Roksund Hov, Espen Melum, Janis Klovins, Elin Org, Gediminas Kiudelis, Juozas Kupcinskas, Jurgita Skieceviciene
Recent studies highlight the presence of bacterial sequences in the human blood, suggesting potential clinical significance for circulating microbial signatures. These sequences could presumably serve in the diagnosis, prediction, or monitoring of various health conditions. Ensuring the similarity of samples before bacterial analysis is crucial, especially when combining samples from different biobanks prepared under varying conditions (such as different DNA extraction kits, centrifugation conditions, blood collection tubes, etc...
February 27, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38393689/cryopreservation-and-biobanking-of-gametes-embryos-and-reproductive-tissues
#13
EDITORIAL
Frank Gao, Ruidong Ma, Shen Ren, Dayong Gao, Zhiquan Shu
No abstract text is available yet for this article.
February 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38393688/acknowledgment-of-reviewers-2023
#14
JOURNAL ARTICLE
(no author information available yet)
No abstract text is available yet for this article.
February 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38393687/isber-2024-annual-meeting-dreaming-beyond-barriers-the-future-of-biobanking
#15
JOURNAL ARTICLE
Cassandra Griffin, Gregory Grossman, Amanda Moors
No abstract text is available yet for this article.
February 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38346330/applying-findable-accessible-interoperable-and-reusable-principles-to-biospecimens-and-biobanks
#16
JOURNAL ARTICLE
Amanda Rush, Jennifer A Byrne, Peter H Watson
The importance of stimulating greater sharing of data for use and reuse in health research is widely recognized. To this end, the findable, accessible, interoperable, and reusable (FAIR) principles for data have been developed and widely accepted in the research community. Research biospecimens are a resource that leads to much of this health research data but are also a form of data. Therefore, the FAIR principles should apply to biospecimens. Nevertheless, there is a widespread problem of not sharing biospecimen resources that is clearly visible within the research arena...
February 12, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38265832/evaluation-of-cryopreserved-ram-sperm-with-nano-ozone-solution-and-post-thaw-life-span-by-flow-cytometric-analysis
#17
JOURNAL ARTICLE
Mehmed Berk Toker, Ahmet Ümit Sabancı, Gülcan Avcı, Ahmet Aktar, Barış Denk, Özge Bari, Gözde Rabia Özalp
Ozone has been used as a therapy tool in medical science for conditions such as ulcers, peritonitis, wounds, and mostly joint problems. Ozone therapy strengthens the resistance to infections by kick-starting antioxidant, anti-inflammatory, and immune modulation systems. Ozone creates a defensive response against oxidative stress in membranes and protects metabolism against reactive oxygen species (ROS). Sperm membranes are one of ROS's main targets; therefore, the cells' cryopreservation process requires more defensive elements for better results...
January 24, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38265831/cryopreservation-induced-morphological-changes-in-freshwater-fish-sperm-a-systematic-review
#18
JOURNAL ARTICLE
Bruna Bitencourt da Costa, Paula Graziela Lassen, Danilo Pedro Streit
A systematic review was performed to summarize the scientific evidence and critically evaluate the effects of cryopreservation on sperm morphology in freshwater fish, and to assess the methodologies for sperm morphology classification. The search strategy was applied to four electronic databases (CAB Direct, Pub Med, Scopus, and ISI Web of Science). The main inclusion criteria involved studies on semen from freshwater fish subjected to the cryopreservation process and evaluation of sperm quality through morphology...
January 24, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38237124/biobanking-legislation-in-spain-advancing-or-undermining-its-ethical-values
#19
JOURNAL ARTICLE
Inmaculada de Melo-Martín, Eva Ortega-Paíno
Biobanks are important resources for improving public health and individual care. Some legal frameworks can be more or less conducive to advancing the potential benefits of biobanks. The purpose of this article is to assess biobanking legislation and practices in Spain to determine how well they fare in such a regard. We focus here on some of the primary ethical values that ground relevant legislation and that we believe are consistent with promoting biobanking benefits: the value of scientific research; efficient use of scarce resources; and respect for the dignity of donors...
January 18, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38227396/vitrification-of-mammalian-oocytes-recent-studies-on-mitochondrial-dysfunction
#20
JOURNAL ARTICLE
Yixiao Zhu, Hongyu Liu, Lv Zheng, Yuwen Luo, Guizhen Zhou, Jun Li, Yunpeng Hou, Xiangwei Fu
Vitrification of reproductive cells is definitely essential and integral in animal breeding, as well as in assisted reproduction. However, issues accompanied with this technology such as decreased oocyte competency and relatively low embryo survival rates appear to be a tough conundrum that has long perplexed us. As significant organelles in cell metabolism, mitochondria play pivotal roles in numerous pathways. Nonetheless, extensive evidence has demonstrated that vitrification can seriously impair mitochondrial function in mammalian oocytes...
January 16, 2024: Biopreservation and Biobanking
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