keyword
MENU ▼
Read by QxMD icon Read
search

lipid—loaded cell

keyword
https://www.readbyqxmd.com/read/28088639/docosahexaenoic-acid-loaded-lipid-nanoparticles-with-bactericidal-activity-against-helicobacter-pylori
#1
Catarina Leal Seabra, Cláudia Nunes, Maria Gomez-Lazaro, Marta Correia, José Carlos Machado, Inês C Gonçalves, Celso A Reis, Salette Reis, M Cristina L Martins
Docosahexaenoic acid (DHA), an omega-3 polyunsaturated fatty acid present in fish oil, has been described as a promising molecule to the treatment of Helicobacter pylori gastric infection. However, due to its highly unsaturated structure, DHA can be easily oxidized loosing part of its bioactivity. This work aims the nanoencapsulation of DHA to improve its bactericidal efficacy against H. pylori. DHA was loaded into nanostructured lipid carriers (NLC) produced by hot homogenization and ultrasonication using a blend of lipids (Precirol ATO5(®), Miglyol-812(®)) and a surfactant (Tween 60(®))...
January 11, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28088205/quercetin-improves-macrophage-reverse-cholesterol-transport-in-apolipoprotein-e-deficient-mice-fed-a-high-fat-diet
#2
Yingjie Cui, Pengbo Hou, Fahui Li, Qinghua Liu, Shucun Qin, Guanghai Zhou, Xuelian Xu, Yanhong Si, Shoudong Guo
BACKGROUND: Quercetin, one of the most widely distributed flavonoids in plants, has been demonstrated to reduce hyperlipidaemia and atherosclerotic lesion formation. Reverse cholesterol transport (RCT) plays a crucial role in exporting cholesterol from peripheral cells, which is one mechanism utilized in the prevention and treatment of atherosclerosis. The aim of this study is to investigate whether quercetin reduces lipid accumulation by improving RCT in vivo. METHODS: Apolipoprotein E-deficient mice fed a high-fat diet were used to investigate the effect of quercetin on RCT by an isotope tracing method, and the underlying mechanisms were clarified by molecular techniques...
January 14, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28081459/targeted-delivery-of-in-situ-pcr-amplified-sleeping-beauty-transposon-genes-to-cancer-cells-with-lipid-based-nanoparticle-like-protocells
#3
Kun Ma, Duo Fu, Dongli Yu, Changhao Cui, Li Wang, Zhaoming Guo, Chuanbin Mao
A Sleeping Beauty (SB) transposon system is made of a transposon plasmid (containing gene encoding a desired functional or therapeutic protein) and a transposase plasmid (encoding an enzyme capable of cutting and pasting the gene into the host cell genome). It is a kind of natural, nonviral gene delivery vehicle, which can achieve efficient genomic insertion, providing long-term transgenic expression. However, before the SB transposon system could play a role in promoting gene expression, it has to be delivered efficiently first across cell membrane and then into cell nuclei...
January 2, 2017: Biomaterials
https://www.readbyqxmd.com/read/28072841/a-small-insulinomimetic-molecule-also-improves-insulin-sensitivity-in-diabetic-mice
#4
Sandip Mukherjee, Mrittika Chattopadhyay, Sushmita Bhattacharya, Suman Dasgupta, Sahid Hussain, Saitanya K Bharadwaj, Dhrubajyoti Talukdar, Abul Usmani, Bhola S Pradhan, Subeer S Majumdar, Pronobesh Chattopadhyay, Satinath Mukhopadhyay, Tushar K Maity, Mihir K Chaudhuri, Samir Bhattacharya
Dramatic increase of diabetes over the globe is in tandem with the increase in insulin requirement. This is because destruction and dysfunction of pancreatic β-cells are of common occurrence in both Type1 diabetes and Type2 diabetes, and insulin injection becomes a compulsion. Because of several problems associated with insulin injection, orally active insulin mimetic compounds would be ideal substitute. Here we report a small molecule, a peroxyvanadate compound i.e. DmpzH[VO(O2)2(dmpz)], henceforth referred as dmp, which specifically binds to insulin receptor with considerable affinity (KD-1...
2017: PloS One
https://www.readbyqxmd.com/read/28071140/development-and-characterization-of-polymer-lipid-hybrid-nanoparticles-for-oral-delivery-of-lmwh
#5
Supandeep Singh Hallan, Veerpal Kaur, Vikas Jain, Neeraj Mishra
The present study aimed to develop an improved oral delivery system for low-molecular-weight heparin (LMWH), novel polymer lipid hybrid nanoparticles were developed. LMWH loaded chitosan polymer lipid hybrid nanoparticles (LMWH-CS-PLNs) were developed using double emulsification and solvent evaporation method. The performance of developed formulations was evaluated by using in vitro and in vivo behavior, such as drug release studies, in vitro permeation study, in vivo venous thrombolytic study, in vitro uptake studies by using intestinal epithelium resembling Caco-2 cell lines...
January 10, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28068880/low-intensity-vibrations-normalize-adipogenesis-induced-morphological-and-molecular-changes-of-adult-mesenchymal-stem-cells
#6
Oznur Baskan, Gulistan Mese, Engin Ozcivici
Bone marrow mesenchymal stem cells that are committed to adipogenesis were exposed daily to high-frequency low-intensity mechanical vibrations to understand molecular, morphological and ultrastructural adaptations to mechanical signals during adipogenesis. D1-ORL-UVA mouse bone marrow mesenchymal stem cells were cultured with either growth or adipogenic medium for 1 week. Low-intensity vibration signals (15 min/day, 90 Hz, 0.1 g) were applied to one group of adipogenic cells, while the other adipogenic group served as a sham control...
January 1, 2017: Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine
https://www.readbyqxmd.com/read/28067223/the-microbiota-maintain-homeostasis-of-liver-resident-%C3%AE-%C3%AE-t-17-cells-in-a-lipid-antigen-cd1d-dependent-manner
#7
Fenglei Li, Xiaolei Hao, Yongyan Chen, Li Bai, Xiang Gao, Zhexiong Lian, Haiming Wei, Rui Sun, Zhigang Tian
The microbiota control regional immunity using mechanisms such as inducing IL-17A-producing γδ T (γδT-17) cells in various tissues. However, little is known regarding hepatic γδT cells that are constantly stimulated by gut commensal microbes. Here we show hepatic γδT cells are liver-resident cells and predominant producers of IL-17A. The microbiota sustain hepatic γδT-17 cell homeostasis, including activation, survival and proliferation. The global commensal quantity affects the number of liver-resident γδT-17 cells; indeed, E...
January 9, 2017: Nature Communications
https://www.readbyqxmd.com/read/28065730/potential-of-cecl3-msio2-nanoparticles-in-alleviating-diabetic-cataract-development-and-progression
#8
Jin Yang, Xiaoqun Gong, Lei Fang, Qi Fan, Lei Cai, Xiaodi Qiu, Bo Zhang, Jin Chang, Yi Lu
Cataract is a major cause of visual impairment for diabetic patients. It is imperative to develop efficient therapeutic agents against diabetic cataract (DC) because diabetes confers higher risk for complications after cataract surgery. We have previously reported the role of CeCl3 loaded mesoporous silica (CeCl3@mSiO2) nanoparticles in reducing the oxidative stress of lens epithelial cells. However, the potential of CeCl3@mSiO2 in preventing diabetic cataract development remains unclear. In this study, we applied CeCl3@mSiO2 nanoparticles with a size of 87...
January 5, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28063902/application-of-aluminum-chloride-phthalocyanine-loaded-solid-lipid-nanoparticles-for-photodynamic-inactivation-of-melanoma-cells
#9
Patrícia L Goto, Marigilson P Siqueira-Moura, Antonio C Tedesco
Cutaneous melanoma is the most aggressive skin cancer and is particularly resistant to current therapeutic approaches. Photodynamic therapy (PDT) is a well-established photoprocess that is employed to treat some cancers, including non-melanoma skin cancer. Aluminum chloride phthalocyanine (ClAlPc) is used as a photosensitizer in PDT; however, its high hydrophobicity hampers its photodynamic activity under physiological conditions. The aim of this study was to produce solid lipid nanoparticles (SLN) containing ClAlPc using the direct emulsification method...
January 4, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28053531/enhanced-photocytotoxicity-of-curcumin-delivered-by-solid-lipid-nanoparticles
#10
Shan Jiang, Rongrong Zhu, Xiaolie He, Jiao Wang, Mei Wang, Yechang Qian, Shilong Wang
Curcumin (Cur) is a promising photosensitizer that could be used in photodynamic therapy. However, its poor solubility and hydrolytic instability limit its clinical use. The aim of the present study was to encapsulate Cur into solid lipid nanoparticles (SLNs) in order to improve its therapeutic activity. The Cur-loaded SLNs (Cur-SLNs) were prepared using an emulsification and low-temperature solidification method. The functions of Cur and Cur-SLNs were studied on the non-small cell lung cancer A549 cells for photodynamic therapy...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28049043/perfluoroalkyl-acids-induced-liver-steatosis-effects-on-genes-controlling-lipid-homeostasis
#11
Kaberi P Das, Carmen R Wood, Mimi J Lin, Anatoly A Starkov, Christopher Lau, Kendall B Wallace, J Christopher Corton, Barbara D Abbott
Persistent presence of perfluoroalkyl acids (PFAAs) in the environment is due to their extensive use in industrial and consumer products, and their slow decay. Biochemical tests in rodent demonstrated that these chemicals are potent modifiers of lipid metabolism and cause hepatocellular steatosis. However, the molecular mechanism of PFAAs interference with lipid metabolism remains to be elucidated. Currently, two major hypotheses are that PFAAs interfere with mitochondrial beta-oxidation of fatty acids and/or they affect the transcriptional activity of peroxisome proliferator-activated receptor α (PPARα) in liver...
December 31, 2016: Toxicology
https://www.readbyqxmd.com/read/28045496/magnetic-nanoliposomes-as-in-situ-microbubble-bombers-for-multimodality-image-guided-cancer-theranostics
#12
Yang Liu, Fang Yang, Chuxiao Yuan, Mingxi Li, Tuantuan Wang, Bo Chen, Juan Jin, Peng Zhao, Jiayi Tong, Shouhua Luo, Ning Gu
Nano-sized drug delivery systems have offered promising approaches for cancer theranostics. However, few are effective to simultaneously maximize tumor specific uptake, imaging and therapy in a single nanoplatform. Here, we report a simple yet stimuli-responsive anethole dithiolethione (ADT) loaded magnetic nanoliposomes (AMLs) delivery system, which consists of ADT, hydrogen sulfide (H2S) pro-drug, doped in the lipid bilayer, and superparamagnetic nanoparticles (MNPs) encapsulated inside. HepG2 cells could be effectively bombed after 6 h co-incubation with AMLs...
January 3, 2017: ACS Nano
https://www.readbyqxmd.com/read/28041717/the-effect-of-cholesterol-loaded-cyclodextrins-on-post-thawing-quality-of-buffalo-semen-in-relation-to-sperm-dna-damage-and-ultrastructure
#13
M F Abouelezz, A E Montaser, M S Hussein, A M Eldesouky, M R Badr, A O Hegab, A Z Balboula, S M Zaabel
The cryopreservation of germ cells is a major tool for the propagation of animals with desired genetic traits. Although cryopreservation of spermatozoa in some animals is effective, its effectiveness is variable. For example, cryopreservation efficiency of buffalo bull spermatozoa remains very poor. In this study, we evaluated sperm DNA damage and ultrastructure in buffalo bull spermatozoa vitrified in the presence or absence of cholesterol-loaded cyclodextrins (CLC). Our results showed that cryopreserved buffalo spermatozoa had elevated levels of deteriorated plasma and mitochondrial membranes, which are the likely causes of DNA damage after vitrification...
December 29, 2016: Reproductive Biology
https://www.readbyqxmd.com/read/28032534/improved-oral-bioavailability-and-therapeutic-efficacy-of-dabigatran-etexilate-via-soluplus-%C3%A2-tpgs-binary-mixed-micelles-system
#14
Mei Hu, Jinjie Zhang, Rui Ding, Yao Fu, Tao Gong, Zhirong Zhang
The clinical use of dabigatran etexilate (DABE) is limited by its poor absorption and relatively low bioavailability. Our study aimed to explore the potential of a mixed micelle system composed of Soluplus(®) and D-alpha tocopheryl polyethylene glycol 1000 succinate (TPGS) to improve the oral absorption and bioavailability of DBAE. DBAE was first encapsulated into Soluplus/TPGS mixed micelles by a simple thin film hydration method. The DBAE loaded micelles displayed an average size distribution of around 83...
December 29, 2016: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28027539/construction-and-comparison-of-different-nanocarriers-for-co-delivery-of-cisplatin-and-curcumin-a-synergistic-combination-nanotherapy-for-cervical-cancer
#15
Changming Li, Xiangcheng Ge, Liguo Wang
PURPOSE: Co-delivery of two or more drugs into the same cancer cells or tissues in the same nanocarriers provides a new paradigm in cancer treatment. In this study, two kinds of nanocarriers: lipid-polymer hybrid nanoparticles (LPNs) and polymeric nanoparticles (PNPs) were constructed and compared for co-delivery of cisplatin (DDP) and curcumin (CUR). METHODS: DDP and CUR loaded LPNs (D/C/LPNs) and PNPs (D/C/PNPs) were prepared. Two kinds of nanocarriers were characterized in terms of particle size, zeta potential, drug encapsulation efficiency (EE), and drug release...
December 24, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28025072/enhancing-biopharmaceutical-attributes-of-phospholipid-complex-loaded-nanostructured-lipidic-carriers-of-mangiferin-systematic-development-characterization-and-evaluation
#16
Rajneet Kaur Khurana, Arvind K Bansal, Sarwar Beg, Andrea Julie Burrow, O P Katare, Kamalinder K Singh, Bhupinder Singh
Mangiferin (Mgf), largely expressed out from the leaves and stem bark of Mango, is a potent antioxidant. However, its in vivo activity gets tremendously reduced owing to poor aqueous solubility and inconsistent gastrointestinal absorption, high hepatic first-pass metabolism and high P-gp efflux. The current research work, therefore, was undertaken to overcome the biopharmaceutical hiccups by developing the Mgf-phospholipid complex (PLCs) loaded in nanostructured lipidic carriers (NLCs). The PLCs and NLCs were prepared using refluxing, solvent evaporation and hot emulsification technique, respectively with three molar ratios of Mgf and Phospholipon 90G, i...
December 23, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28024291/novel-transferrin-modified-and-doxorubicin-loaded-pluronic-85-lipid-polymeric-nanoparticles-for-the-treatment-of-leukemia-in-vitro-and-in-vivo-therapeutic-effect-evaluation
#17
Baomin Zhu, Huanying Zhang, Lianling Yu
PURPOSE: Childhood leukemia is a common malignant disease in children. Doxorubicin (DOX) was widely used for the treatment of leukemia. However, severe toxic side effects and drug resistance are the major limitations of DOX. Nanocarriers offer the opportunity to overcome these drawbacks, there are many attempts to enhance the activity of DOX against drug resistance. This study aimed to develop a novel transferrin (Tf) modified and doxorubicin (DOX) loaded Pluronic 85/lipid-polymeric nanoparticles for the treatment of leukemia...
December 23, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28013140/assessment-of-municipal-wastewaters-at-various-stages-of-treatment-process-as-potential-growth-media-for-chlorella-sorokiniana-under-different-modes-of-cultivation
#18
Prathana Ramsundar, Abhishek Guldhe, Poonam Singh, Faizal Bux
Wastewater utilization for microalgal biomass production is potentially the most economical route for its fuel and feed applications. In this study, suitability of various wastewater streams within a domestic wastewater treatment plant was evaluated for microalgal cultivation. Pre-treatment methods were evaluated to minimize bacterial load. Biomass, cell physiology, nutrient removal efficiencies and biochemical constituents of Chlorella sorokiniana were investigated in influent (INF) and anaerobic tank centrate (AC) under mixotrophic (Mixo) and heterotrophic (Hetero) cultivation...
March 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28011344/dermal-quercetin-lipid-nanocapsules-influence-of-the-formulation-on-antioxidant-activity-and-cellular-protection-against-hydrogen-peroxide
#19
T Hatahet, M Morille, A Shamseddin, A Aubert-Pouëssel, J M Devoisselle, S Bégu
Quercetin is a plant flavonoid with strong antioxidant and antiinflammatory properties interesting for skin protection. However, its poor water solubility limits its penetration and so its efficiency on skin. For this purpose, quercetin lipid nanocapsules were formulated implementing phase inversion technique wherein several modifications were introduced to enhance quercetin loading. Quercetin lipid nanocapsules were formulated with two particle size range, (50nm and 20nm) allowing a drug loading of 18.6 and 32mM respectively...
December 21, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28008422/pathogenic-mycobacteria-achieve-cellular-persistence-by-inhibiting-the-niemann-pick-type-c-disease-cellular-pathway
#20
Paul Fineran, Emyr Lloyd-Evans, Nathan A Lack, Nick Platt, Lianne C Davis, Anthony J Morgan, Doris Höglinger, Raju Venkata V Tatituri, Simon Clark, Ian M Williams, Patricia Tynan, Nada Al Eisa, Evgeniya Nazarova, Ann Williams, Antony Galione, Daniel S Ory, Gurdyal S Besra, David G Russell, Michael B Brenner, Edith Sim, Frances M Platt
BACKGROUND: Tuberculosis remains a major global health concern. The ability to prevent phagosome-lysosome fusion is a key mechanism by which intracellular mycobacteria, including Mycobacterium tuberculosis, achieve long-term persistence within host cells. The mechanisms underpinning this key intracellular pro-survival strategy remain incompletely understood. Host macrophages infected with persistent mycobacteria share phenotypic similarities with cells taken from patients suffering from Niemann-Pick Disease Type C (NPC), a rare lysosomal storage disease in which endocytic trafficking defects and lipid accumulation within the lysosome lead to cell dysfunction and cell death...
November 18, 2016: Wellcome Open Res
keyword
keyword
35845
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"