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https://www.readbyqxmd.com/read/28433899/sustainable-production-of-a-new-generation-biofuel-by-lipase-catalyzed-esterification-of-fatty-acids-from-liquid-industrial-waste-biomass
#1
Athanasios Foukis, Olga A Gkini, Panagiota-Yiolanda Stergiou, Vasilios A Sakkas, Agapi Dima, Konstantina Boura, Athanasios Koutinas, Emmanuel M Papamichael
In this work we suggest a methodology comprising the design and use of cost-effective, sustainable, and environmentally friendly process for biofuel production compatible with the market demands. A new generation biofuel is produced using fatty acids, which were generated from acidogenesis of industrial wastes of bioethanol distilleries, and esterified with selected alcohols by immobilized Candida antarctica Lipase-B. Suitable reactors with significant parameters and conditions were studied through experimental design, and novel esterification processes were suggested; among others, the continuous removal of the produced water was provided...
April 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28433187/a-study-of-co-syngas-bioconversion-by-clostridium-autoethanogenum-with-a-flexible-gas-cultivation-system
#2
Huijuan Xu, Cuiyi Liang, Zhenhong Yuan, Jingliang Xu, Qiang Hua, Ying Guo
Bioconversion of CO/syngas to produce ethanol is a novel route in bioethanol production, which can be accomplished by some acetogens. Specific culture vessels and techniques are needed to cultivate these microorganisms since they are anaerobic and substrates are gaseous. In this work, gas-sampling bag was applied as a gas-cultivation system to study CO/syngas bioconversion by Clostridium autoethanogenum and was demonstrated to be efficient because of its flexibility and excellent ability to maintain the headspace atmosphere...
June 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28432390/biomass-resources-agriculture
#3
Ingeborg N Kluts, Marnix L J Brinkman, Sierk A de Jong, H Martin Junginger
Bioenergy is the single largest source of renewable energy in the European Union (EU-28); of this, 14% was produced from agricultural feedstocks in 2012. This chapter provides an overview of the current use (for bioenergy) and future potential of agricultural feedstocks for (amongst others) biorefinery purposes in the European Union. The main application of these feedstocks is currently the production of biofuels for road transport. Biodiesel makes up 80% of the European biofuel production, mainly from rapeseed oil, and the remaining part is bioethanol from wheat and sugar beet...
April 22, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28429154/developing-elite-neurospora-crassa-strains-for-cellulosic-ethanol-production-using-fungal-breeding
#4
Joshua C Waters, Andrew Nixon, Morgan Dwyer, Justin C Biffinger, Kwangwon Lee
The demand for renewable and sustainable energy has generated considerable interest in the conversion of cellulosic biomass into liquid fuels such as ethanol using a filamentous fungus. While attempts have been made to study cellulose metabolism through the use of knock-out mutants, there have been no systematic effort to characterize natural variation for cellulose metabolism in ecotypes adapted to different habitats. Here, we characterized natural variation in saccharification of cellulose and fermentation in 73 ecotypes and 89 laboratory strains of the model fungus Neurospora crassa...
April 20, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28413447/enhancement-of-ethanol-production-from-green-liquor-ethanol-pretreated-sugarcane-bagasse-by-glucose-xylose-cofermentation-at-high-solid-loadings-with-mixed-saccharomyces-cerevisiae-strains
#5
Yanzhi You, Pengfei Li, Fuhou Lei, Yang Xing, Jianxin Jiang
BACKGROUND: Efficient cofermentation of glucose and xylose is necessary for economically feasible bioethanol production from lignocellulosic biomass. Here, we demonstrate pretreatment of sugarcane bagasse (SCB) with green liquor (GL) combined with ethanol (GL-Ethanol) by adding different GL amounts. The common Saccharomyces cerevisiae (CSC) and thermophilic S. cerevisiae (TSC) strains were used and different yeast cell mass ratios (CSC to TSC) were compared. The simultaneous saccharification and cofermentation (SSF/SSCF) process was performed by 5-20% (w/v) dry substrate (DS) solid loadings to determine optimal conditions for the co-consumption of glucose and xylose...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28411492/evaluation-of-an-integrated-process-to-fully-utilize-bamboo-biomass-during-the-production-of-bioethanol
#6
Zhaoyang Yuan, Yangbing Wen
Aiming for the complete utilization of bamboo biomass, an integrated process which combines ethanol production with the recovery of silica and lignin was proposed. To reduce chemical charge required for the fractionation of silica and lignin from bamboo and improve the digestibility of the obtained substrate, a sequentially two-stage pretreatment process of autohydrolysis and alkaline extraction was carried out. From the view of enhancing enzymatic hydrolysis and recovery of silica and lignin, a two-stage treatment of autohydrolysis at 180°C for 90min followed by alkaline extraction at 100°C with 6% NaOH (based on pretreated chips) for 120min was optimized...
April 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28411491/production-of-bio-sugar-and-bioethanol-from-coffee-residue-cr-by-acid-chlorite-pretreatment
#7
Ho Myeong Kim, Yong-Soo Choi, Dae-Seok Lee, Yong-Hwan Kim, Hyeun-Jong Bae
Nowadays, coffee residue (CR) after roasting is recognized as one of the most useful resources in the world for producing the biofuel and bio-materials. In this study, we evaluated the potential of bio-sugar and bioethanol production from acid-chlorite treated CR. Notably, CR treated three times with acid-chlorite after organic solvent extraction (OSE-3), showed the high monosaccharide content, and the efficient sugar conversion yield compared to the other pretreatment conditions. The OSE-3 (6% substrate loading, w/v) can produce bio-sugar (0...
March 28, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28409424/enhanced-saccharification-of-lignocellulosic-agricultural-biomass-and-increased-bioethanol-titre-using-acclimated-clostridium-thermocellum-dsm1313
#8
M Nisha, K Saranyah, Mukund Shankar, L M Saleena
Consolidated bioprocess assures an efficient lignocellulosic conversion to fermentable sugars and subsequently to bioethanol. Such a single-step hydrolysis and anaerobic fermentation was achieved with acclimated Clostridium thermocellum DSM 1313 on different mildly pre-treated agricultural lignocellulosic residues without any additional enzymes/and strains. Acclimation was achieved by serially sub-culturing in increasing concentration of individual substrates, such as rice husk, sugarcane bagasse, and banana pseudostem in the standard media, with cellobiose as an adjunct...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28405910/omics-analysis-of-acetic-acid-tolerance-in-saccharomyces-cerevisiae
#9
REVIEW
Peng Geng, Liang Zhang, Gui Yang Shi
Acetic acid is an inhibitor in industrial processes such as wine making and bioethanol production from cellulosic hydrolysate. It causes energy depletion, inhibition of metabolic enzyme activity, growth arrest and ethanol productivity losses in Saccharomyces cerevisiae. Therefore, understanding the mechanisms of the yeast responses to acetic acid stress is essential for improving acetic acid tolerance and ethanol production. Although 329 genes associated with acetic acid tolerance have been identified in the Saccharomyces genome and included in the database ( http://www...
May 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28400172/a-biorefinery-based-approach-for-the-production-of-ethanol-from-enzymatically-hydrolysed-cotton-stalks
#10
Meera Christopher, Anil K Mathew, M Kiran Kumar, Ashok Pandey, Rajeev K Sukumaran
Cotton post-harvest residue/cotton stalk (CS) - a major agro-residue in south asian countries was evaluated as a feed stock for bioethanol production. The common thermochemical pretreatment strategies based on dilute acid and alkali and different combinations of biomass hydrolyzing enzymes were evaluated for saccharification of CS biomass. A hydrolytic efficiency of 80% was achieved for alkali treated biomass using cellulase supplemented with beta glucosidase. Recycling of undigested/residual biomass and/or enzyme supported same final sugar concentration as for fresh hydrolytic experiments...
April 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28399848/distinctive-molecular-and-biochemical-characteristics-of-a-glycoside-hydrolase-family-20-%C3%AE-n-acetylglucosaminidase-and-salt-tolerance
#11
Junpei Zhou, Zhifeng Song, Rui Zhang, Rui Liu, Qian Wu, Junjun Li, Xianghua Tang, Bo Xu, Junmei Ding, Nanyu Han, Zunxi Huang
BACKGROUND: Enzymatic degradation of chitin has attracted substantial attention because chitin is an abundant renewable natural resource, second only to lignocellulose, and because of the promising applications of N-acetylglucosamine in the bioethanol, food and pharmaceutical industries. However, the low activity and poor tolerance to salts and N-acetylglucosamine of most reported β-N-acetylglucosaminidases limit their applications. Mining for novel enzymes from new microorganisms is one way to address this problem...
April 11, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28385593/a-genome-scale-model-of-geobacillus-thermoglucosidasius-c56-ys93-reveals-its-biotechnological-potential-on-rice-straw-hydrolysate
#12
Ahmad Ahmad, Hassan B Hartman, S Krishnakumar, David A Fell, Mark G Poolman, Shireesh Srivastava
Rice straw is a major crop residue which is burnt in many countries, creating significant air pollution. Thus, alternative routes for disposal of rice straw are needed. Biotechnological treatment of rice straw hydrolysate has potential to convert this agriculture waste into valuable biofuel(s) and platform chemicals. Geobacillus thermoglucosidasius is a thermophile with properties specially suited for use as a biocatalyst in lignocellulosic bioprocesses, such as high optimal temperature and tolerance to high levels of ethanol...
April 4, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28384593/thermo-acid-stable-phytase-mediated-enhancement-of-bioethanol-production-using-colocasia-esculenta
#13
Melvin Makolomakwa, Adarsh Kumar Puri, Kugen Permaul, Suren Singh
Phytase production by the thermophilic mould Thermomyces lanuginosus SSBP was enhanced 8.56-fold in submerged fermentation, which was further improved in fed-batch cultivations. The protein was purified to homogeneity using ammonium sulphate precipitation, Resource Q anion exchange and Superdex gel-filtration chromatography, with an overall purification of 24.7-fold and a yield of 5.16%. The purified 49kDa protein was optimally active at 55°C and pH 5.0, and was stable between 50 and 90°C from pH 3.0-6.0, with a half-life of 138...
March 29, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28381218/development-of-an-efficient-technique-for-gene-deletion-and-allelic-exchange-in-geobacillus-spp
#14
Leann F Bacon, Charlotte Hamley-Bennett, Michael J Danson, David J Leak
BACKGROUND: Geobacillus thermoglucosidasius is a thermophilic, natural ethanol producer and a potential candidate for commercial bioethanol production. Previously, G. thermoglucosidasius has been genetically modified to create an industrially-relevant ethanol production strain. However, creating chromosomal integrations and deletions in Geobacillus spp. is laborious. Here we describe a new technique to create marker-less mutations in Geobacillus utilising a novel homologous recombination process...
April 5, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28372036/bioethanol-production-from-hydrodictyon-reticulatum-by-fed-batch-fermentation-using-saccharomyces-cerevisiae-kctc7017
#15
Seul Ki Kim, Cuong Mai Nguyen, Eun Hye Ko, In-Chul Kim, Jin-Seog Kim, Jin-Cheol Kim
The aim of this study was to develop a potential process for bioethanol production from Hydrodictyon reticulatum (HR), a filamentous freshwater alga, using Saccharomyces cerevisiae (KCTC7017). From the sugar solutions prepared by the four different hydrolysis methods, bioethanol production ranged from 11.0 g/100 g dried material (acid hydrolysis) to 22.3 g/100 g dried material (enzymatic hydrolysis, EH). Bioethanol was fermented from a highly concentrated sugar solution obtained by decompression-mediated (vacuum) enrichment method (VE)...
April 3, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28366693/cellulase-production-through-solid-state-tray-fermentation-and-its-use-for-bioethanol-from-sorghum-stover
#16
Ayman Salih Omer Idris, Ashok Pandey, S S Rao, Rajeev K Sukumaran
The production of cellulase by Trichoderma reesei RUT C-30 under solid-state fermentation (SSF) on wheat bran and cellulose was optimized employing a two stage statistical design of experiments. Optimization of process parameters resulted in a 3.2-fold increase in CMCase production to 959.53IU/gDS. The process was evaluated at pilot scale in tray fermenters and yielded 457IU/gDS using the lab conditions and indicating possibility for further improvement. The cellulase could effectively hydrolyze alkali pretreated sorghum stover and addition of Aspergillus niger β-glucosidase improved the hydrolytic efficiency 174%, indicating the potential to use this blend for effective saccharification of sorghum stover biomass...
March 20, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28363963/improved-xylose-metabolism-by-a-cyc8-mutant-of-saccharomyces-cerevisiae
#17
Jeroen G Nijland, Hyun Yong Shin, Leonie G M Boender, Paul P de Waal, Paul Klaassen, Arnold J M Driessen
Engineering Saccharomyces cerevisiae for the utilization of pentose sugars is an important goal for the production of second-generation bioethanol and biochemicals. However, S. cerevisiae lacks specific pentose transporters and, in the presence of glucose, pentoses enter the cell inefficiently via endogenous hexose (HXT) transporters. By means of in vivo engineering, we have evolved a quadruple hexokinase deletion mutant of S. cerevisiae into a strain that efficiently utilizes D-xylose in the presence of high D-glucose concentrations...
March 31, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28360937/enhanced-fermentative-performance-under-stresses-of-multiple-lignocellulose-derived-inhibitors-by-overexpression-of-a-typical-2-cys-peroxiredoxin-from-kluyveromyces-marxianus
#18
Jiaoqi Gao, Hualiang Feng, Wenjie Yuan, Yimin Li, Shengbo Hou, Shijun Zhong, Fengwu Bai
BACKGROUND: Bioethanol from lignocellulosic materials is of great significance to the production of renewable fuels due to its wide sources. However, multiple inhibitors generated from pretreatments represent great challenges for its industrial-scale fermentation. Despite the complex toxicity mechanisms, lignocellulose-derived inhibitors have been reported to be related to the levels of intracellular reactive oxygen species (ROS), which makes oxidoreductase a potential target for the enhancement of the tolerance of yeasts to these inhibitors...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28360170/draft-genome-sequence-of-saccharomyces-cerevisiae-barra-grande-bg-1-a-brazilian-industrial-bioethanol-producing-strain
#19
Natalia Coutouné, Aline Tieppo Nogueira Mulato, Diego Mauricio Riaño-Pachón, Juliana Velasco de Castro Oliveira
Here, we present the draft genome sequence of Saccharomyces cerevisiae BG-1, a Brazilian industrial strain widely used for bioethanol production from sugarcane. The 11.7-Mb genome sequence consists of 216 scaffolds and harbors 5,607 predicted protein-coding genes.
March 30, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28351729/valorization-of-carob-waste-definition-of-a-second-generation-bioethanol-production-process
#20
Hajar Bahry, Agnès Pons, Rawa Abdallah, Guillaume Pierre, Cédric Delattre, Nidal Fayad, Samir Taha, Christophe Vial
The aim of this work was to develop a strategy for second-generation ethanol production from carob solid waste issued from Lebanese food industry. The pros and cons of submerged (SF) and solid-state fermentations (SSF) using S. cerevisiae on ethanol yield and productivity were compared, including the respective roles of upstream and downstream processes, such as the size reduction, or sugar and ethanol recovery processes. The design of experiments methodology was applied. Experimental results demonstrated that SSF applied to cut carob waste from carob syrup preparation was simpler to operate and more cost-effective, maintained yield and productivity (0...
March 11, 2017: Bioresource Technology
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