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Borja Lagoa-Costa, Haris Nalakath Abubackar, María Fernández-Romasanta, Christian Kennes, María C Veiga
Syngas bioconversion is a promising method for bioethanol production, but some VFA remains at the end of fermentation. A two-stage process was set-up, including syngas fermentation as first stage under strict anaerobic conditions using C. autoethanogenum as inoculum, with syngas (CO/CO2/H2/N2, 30/10/20/40) as gaseous substrate. The second stage consisted in various fed-batch assays using a highly enriched PHA accumulating biomass as inoculum, where the potential for biopolymer production from the remaining acetic acid at the end of the syngas fermentation was evaluated...
May 5, 2017: Bioresource Technology
Chun-Han Ko, Fan-Chun Yu, Fang-Chih Chang, Bing-Yuan Yang, Wen-Hua Chen, Wen-Song Hwang, Ta-Chih Tu
Using plants to absorb and accumulate heavy metals from polluted soil, followed by the recycling of explants containing heavy metals, can help achieve the goal of reverting contaminated soil to low heavy-metal content soil. However, the re-use of recovered explants can also be problematic. Meanwhile, bioethanol has become a popular energy source. In this study, napier grass was used for the remediation of soil contaminated with heavy metals (artificially contaminated soil). The influence of bioethanol production from napier grass after phytoremediation was also investigated...
May 11, 2017: Journal of Environmental Management
Ming-Ju Wu, Guan-Fu Ye, Ching-Hao Wang, Hong-Ting Victor Lin, Chien-Chung Chen, Cheng-Huang Lin
Hollow, poly(L-lactic acid) microtube array membranes (MTAM) were used in preparing membranes that contained immobilized yeast cells. To evaluate the performance of the developed system for continuous and fed-batch fermentation, a gas chromatography/milli-whistle device was used to on-line monitor the production of ethanol. The milli-whistle was connected to the outlet of a GC capillary, and when the fermentation gases and the GC carrier gas passed through it, a sound with a fundamental frequency was produced...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
Mohammad Perwez, Razi Ahmad, Meryam Sardar
A multipurpose magnetic nanobiocatalyst is developed by conjugating Pectinex 3XL (a commercial enzyme containing pectinase, xylanase and cellulase activities) on 3-aminopropyl triethoxysilane activated magnetic nanoparticles. The nanobiocatalyst retained 87% of pectinase, 69% of xylanase and 58% of cellulase activity after conjugation on modified nanoparticles as compared to their soluble counterparts. Thermal stability data at 70°C showed increase in enzyme stability after conjugation to nanoparticles and the kinetic parameters (Km and Vmax) remain unaltered after immobilization...
May 8, 2017: International Journal of Biological Macromolecules
Suryang Kwak, Yong-Su Jin
Efficient xylose utilization is one of the most important pre-requisites for developing an economic microbial conversion process of terrestrial lignocellulosic biomass into biofuels and biochemicals. A robust ethanol producing yeast Saccharomyces cerevisiae has been engineered with heterologous xylose assimilation pathways. A two-step oxidoreductase pathway consisting of NAD(P)H-linked xylose reductase and NAD(+)-linked xylitol dehydrogenase, and one-step isomerase pathway using xylose isomerase have been employed to enable xylose assimilation in engineered S...
May 11, 2017: Microbial Cell Factories
Gang Gao, Run-Qian Mao, Yue Xiao, Jing Zhou, Yu-Huan Liu, Gang Li
The endoglucanase gene endo753 from Aspergillus flavus NRRL3357 strains was cloned, and the recombinant Endo753 was displayed on the cell surface of Saccharomyces cerevisiae EBY100 strain by the C-terminal fusion using Aga2p protein as anchor attachment tag. The results of indirect immunofluorescence and Western blot confirmed the expression and localization of Endo753 on the yeast cell surface. The hydrolytic activity test of the whole-cell enzyme revealed that Endo753 immobilized on the yeast cell surface had high endoglucanase activity...
June 2017: World Journal of Microbiology & Biotechnology
Kengo Sasaki, Yota Tsuge, Hideo Kawaguchi, Masahiro Yasukawa, Daisuke Sasaki, Takashi Sazuka, Eiji Kamio, Chiaki Ogino, Hideto Matsuyama, Akihiko Kondo
The juice from sweet sorghum cultivar SIL-05 (harvested at physiological maturity) was extracted, and the component sucrose and reducing sugars (such as glucose and fructose) were subjected to a membrane separation process to purify the sucrose for subsequent sugar refining and to obtain a feedstock for repeated bioethanol production. Nanofiltration (NF) of an ultrafiltration (UF) permeate using an NTR-7450 membrane (Nitto Denko Corporation, Osaka, Japan) concentrated the juice and produced a sucrose-rich fraction (143...
May 10, 2017: Applied Microbiology and Biotechnology
Rachana Singh, Parul Parihar, Madhulika Singh, Andrzej Bajguz, Jitendra Kumar, Samiksha Singh, Vijay P Singh, Sheo M Prasad
Cyanobacteria and algae having complex photosynthetic systems can channelize absorbed solar energy into other forms of energy for production of food and metabolites. In addition, they are promising biocatalysts and can be used in the field of "white biotechnology" for enhancing the sustainable production of food, metabolites, and green energy sources such as biodiesel. In this review, an endeavor has been made to uncover the significance of various metabolites like phenolics, phytoene/terpenoids, phytols, sterols, free fatty acids, photoprotective compounds (MAAs, scytonemin, carotenoids, polysaccharides, halogenated compounds, etc...
2017: Frontiers in Microbiology
Venkatachalam Narayanan, Jenny Schelin, Marie Gorwa-Grauslund, Ed Wj van Niel, Magnus Carlquist
BACKGROUND: Production of second-generation bioethanol and other bulk chemicals by yeast fermentation requires cells that tolerate inhibitory lignocellulosic compounds at low pH. Saccharomyces cerevisiae displays high plasticity with regard to inhibitor tolerance, and adaptation of cell populations to process conditions is essential for reaching efficient and robust fermentations. RESULTS: In this study, we assessed responses of isogenic yeast cell populations in different physiological states to combinations of acetic acid, vanillin and furfural at low pH...
2017: Biotechnology for Biofuels
Shuo-Fu Yuan, Gia-Luen Guo, Wen-Song Hwang
Renewable and low-cost lignocellulosic wastes have attractive applications in bioethanol production. The yeast Saccharomyces cerevisiae is the most widely used ethanol-producing microbe; however, its fermentation temperature (30-35°C) is not optimum (40-50°C) for enzymatic hydrolysis in the simultaneous saccharification and fermentation (SSF) process. In this study, we successfully performed an SSF process at 42°C from a high solid loading of 20% (w/v) acid-impregnated steam explosion (AISE)-treated rice straw with low inhibitor concentrations (furfural 0...
May 5, 2017: Microbial Biotechnology
M C Jonathan, J DeMartini, S Van Stigt Thans, R Hommes, M A Kabel
BACKGROUND: Corn stover is lignocellulosic biomass that has potential to be used as raw material for bioethanol production. In the current research, dilute ammonia pretreatment was used to improve the accessibility of corn stover carbohydrates to subsequently added hydrolytic enzymes. Some carbohydrates, however, were still present after enzymatic hydrolysis and fermentation. Hence, this research was aimed to characterise the recalcitrant carbohydrates, especially the oligosaccharides that remained after hydrolysis and fermentation of dilute ammonia-pretreated corn stover (DACS)...
2017: Biotechnology for Biofuels
Sandeep K Panda, Ramesh C Ray, Swati S Mishra, Eugenie Kayitesi
The review focuses on some of the high value-end biocommodities, such as fermented beverages, single-cell proteins, single-cell oils, biocolors, flavors, fragrances, polysaccharides, biopesticides, plant growth regulators, bioethanol, biogas and biohydrogen, developed from the microbial processing of fruit and vegetable wastes. Microbial detoxification of fruit and vegetable processing effluents is briefly described. The advances in genetic engineering of microorganisms for enhanced yield of the above-mentioned biocommodities are elucidated with selected examples...
May 2, 2017: Critical Reviews in Biotechnology
Chengqiang Wang, Jianzhi Zhao, Chenxi Qiu, Shihao Wang, Yu Shen, Binghai Du, Yanqin Ding, Xiaoming Bao
Efficient and cost-effective fuel ethanol production from lignocellulosic materials requires simultaneous cofermentation of all hydrolyzed sugars, mainly including D-glucose, D-xylose, and L-arabinose. Saccharomyces cerevisiae is a traditional D-glucose fermenting strain and could utilize D-xylose and L-arabinose after introducing the initial metabolic pathways. The efficiency and simultaneous coutilization of the two pentoses and D-glucose for ethanol production in S. cerevisiae still need to be optimized...
2017: BioMed Research International
Yoko Ishida, Trinh Thi My Nguyen, Shingo Izawa
Lignocellulosic biomass conversion inhibitors such as vanillin, furfural, and 5-hydroxymethylfurfural (HMF) inhibit the growth of and fermentation by Saccharomyces cerevisiae. A high concentration of each fermentation inhibitor represses translation and increases non-translated mRNAs. We previously reported that the mRNAs of ADH7 and BDH2, which encode putative NADPH- and NADH-dependent alcohol dehydrogenases, respectively, were efficiently translated even with translation repression in response to severe vanillin stress...
April 27, 2017: Journal of Biotechnology
In Yung Sunwoo, Jung Eun Kwon, Trung Hau Nguyen, Chae Hun Ra, Gwi-Taek Jeong, Sung-Koo Kim
Conditions for ethanol production were evaluated using waste seaweed obtained from Gwangalli beach, Busan, Korea, after strong winds on January 15, 2015. Eleven types of seaweed were identified, and the proportions of red, brown, and green seaweed wastes were 26, 46, and 28%, respectively. Optimal pretreatment conditions were determined as 8% slurry content, 286 mM H2SO4 for 90 min at 121 °C. Enzymatic saccharification with 16 units/mL Celluclast 1.5L and Viscozyme L mixture at 45 °C for 48 h was carried out as optimal condition...
April 29, 2017: Applied Biochemistry and Biotechnology
Elias Luiz de Souza, Noeli Sellin, Cintia Marangoni, Ozair Souza
Pseudostem of the Musa cavendishii banana plant was submitted to chemical pretreatments with acid (H2SO4 2%, 120 °C, 15 min) and with alkali (NaOH 3%, 120 °C, 15 min), saccharified by commercial enzymes Novozymes® (Cellic CTec2 and HTec2). The influences of the pretreatments on the degradation of the lignin, cellulose and hemicellulose, porosity of the surface, particle crystallinity, and yield in reducing sugars after saccharification (Y RS), were established. Different concentrations of biomass (70 and 100 g/L in dry matter (dm)), with different physical differences (dry granulated, crushed wet bagasse, and whole pseudostem), were used...
April 28, 2017: Applied Biochemistry and Biotechnology
Ioannis Papapetridis, Marlous van Dijk, Antonius J A van Maris, Jack T Pronk
BACKGROUND: Glycerol, whose formation contributes to cellular redox balancing and osmoregulation in Saccharomyces cerevisiae, is an important by-product of yeast-based bioethanol production. Replacing the glycerol pathway by an engineered pathway for NAD(+)-dependent acetate reduction has been shown to improve ethanol yields and contribute to detoxification of acetate-containing media. However, the osmosensitivity of glycerol non-producing strains limits their applicability in high-osmolarity industrial processes...
2017: Biotechnology for Biofuels
Qurat-Ul-Ain Ahmad, Shang-Tian Yang, Maleeha Manzoor, Javed Iqbal Qazi
BACKGROUND: Biofuels obtained from first-generation (1G) sugars-starch streams have been proven unsustainable as their constant consumption is not only significantly costly for commercial scale production systems, but it could potentially lead to problems associated with extortionate food items for human usage. In this regard, biofuels' production in alkali-thermophilic environs from second-generation (2G) bio-waste would not only be markedly feasible, but these extreme conditions might be able to sustain aseptic fermentations without spending much for sterilization...
2017: Biotechnology for Biofuels
Ranjan Parajuli, Marie Trydeman Knudsen, Morten Birkved, Sylvestre Njakou Djomo, Andrea Corona, Tommy Dalgaard
This study evaluates the environmental impacts of biorefinery products using consequential (CLCA) and attributional (ALCA) life cycle assessment (LCA) approaches. Within ALCA, economic allocation method was used to distribute impacts among the main products and the coproducts, whereas within the CLCA system expansion was adopted to avoid allocation. The study seeks to answer the questions (i) what is the environmental impacts of process integration?, and (ii) do CLCA and ALCA lead to different conclusions when applied to biorefinery?...
April 25, 2017: Science of the Total Environment
Annachiara Forte, Amalia Zucaro, Massimo Fagnano, Angelo Fierro
A life cycle assessment (LCA) was applied to assess the environmental load of a prospective local bioethanol (EtOH) production system in Southern Italy by using lignocellulosic Fiber sorghum (FS) feedstock. A two steps analysis was carried out considering: (i) a "cradle-to-farm gate" LCA to investigate thoroughly the FS cultivation on hilly marginal land and (ii) a "cradle-to-wheels" system boundary encompassing the environmental pressure of the whole EtOH supply-use chain. Primary data related to lignocellulosic biomass production were combined with experimental feedstock conversion processes through advanced second generation technology...
April 24, 2017: Science of the Total Environment
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