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Souvik Kumar Paul, Saikat Chakraborty
Sunn hemp fibre - a cellulose-rich crystalline non-food energy crop, containing 75.6% cellulose, 10.05% hemicellulose, 10.32% lignin, with high crystallinity (80.17%) and degree of polymerization (650) - is identified as a new non-food substrate for lignocellulosic biofuel production. Microwave irradiation is employed to rapidly rupture the cellulose's glycosidic bonds and enhance glucose yield to 78.7% at 160 °C in only 46 min. The reactants - long-chain cellulose, ionic liquid, transition metal catalyst, and water - form a polar supramolecular complex that rotates under the microwave's alternating polarity and rapidly dissipates the electromagnetic energy through molecular collisions, thus accelerating glycosidic bond breakage...
January 3, 2018: Bioresource Technology
Mark Roghair, Tim Hoogstad, David P B T B Strik, Caroline M Plugge, Peer Timmers, Ruud A Weusthuis, Marieke E Bruins, Cees J N Buisman
Chain elongation is an open-culture biotechnological process which converts volatile fatty acids (VFAs) into medium chain fatty acids (MCFAs) using ethanol and other reduced substrates. The objective of this study was to investigate the quantitative effect of CO2 loading rate on ethanol usages in a chain elongation process. We supplied different rates of CO2 to a continuously stirred anaerobic reactor, fed with ethanol and propionate. Ethanol was used to upgrade ethanol itself into caproate or to upgrade the supplied VFA (propionate) into heptanoate...
January 5, 2018: Environmental Science & Technology
Qiuzhuo Zhang, Yan Wei, Hui Han, Chen Weng
This study investigated the possibility of enhancing bioethanol production by combined pretreatment methods for water hyacinth. Three different kinds of pretreatment methods, including microbial pretreatment, microbial combined dilute acid pretreatment, and microbial combined dilute alkaline pretreatment, were investigated for water hyacinth degradation. The results showed that microbial combined dilute acid pretreatment is the most effective method, resulting in the highest cellulose content (39.4 ± 2...
December 27, 2017: Bioresource Technology
Reza Shaghaghi-Moghaddam, Hoda Jafarizadeh-Malmiri, Parviz Mehdikhani, Sepide Jalalian, Reza Alijanianzadeh
Efforts to produce bioethanol with higher productivity in a batch submerged fermentation were made by evaluating the bioethanol production of the five different strains of Saccharomyces cerevisiae, namely, NCYC 4109 (traditional bakery yeast), SFO6 (industrial yeast), TTCC 2956 (hybrid baking yeast) and two wild yeasts, PTCC 5052 and BY 4743. The bioethanol productivity and kinetic parameters for all five yeasts at constant fermentation conditions, during 72 h, were evaluated and monitored. The obtained results indicated that compared to the wild yeasts, both traditional bakery (NCYC 4109) and industrial (SFO6) yeasts had higher bioethanol productivity (0...
December 28, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
Joshua Osuigwe Madu, Bolade Oyeyinka Agboola
Bioethanol is an environmentally friendly alternative to petroleum energy sources. This study evaluated the effects of H2O, HCl, NaOH and FeCl3 pretreated rice husk feedstocks on the production of bioethanol. The pretreatments were carried out using water, 0.1 M HCl, NaOH and FeCl3 at 121 °C for 15 min, followed by simultaneous saccharification and fermentation (SSF) as well as separate hydrolysis and fermentation (SHF). The raw and pretreated lignocellulosic feedstocks were analyzed using Fourier transform infrared spectroscopy...
January 2018: 3 Biotech
Bahiru Tsegaye, Chandrajit Balomajumder, Partha Roy
The combination of NaOH pretreatment and microorganisms isolated from termite were used for releasing the wrapped polysaccharides in wheat straw biomass matrix. Different concentrations of NaOH (1, 3, 5, 7 and 10%) were considered to remove lignin and to release polysaccharides as a pretreatment method at 800C for 4 hours before subjecting it to microbial hydrolysis. Data obtained from compositional analysis of pretreated wheat straws shows significant amount of cellulose and lignin were released after NaOH pretreatments...
December 18, 2017: Environmental Technology
Bárbara Catarina Bastos de Freitas, Eduarda Holz Brächer, Etiele Greque de Morais, Daniel Ibraim Pires Atala, Michele Greque de Morais, Jorge Alberto Vieira Costa
New strategies for reformulating species-specific culture media are of great interest to increase the productivity of microalgae. In the search for alternative carbon sources for microalgae cultivation, pentoses can be considered interesting alternatives since the most abundant global source of renewable biomass is lignocellulosic waste, which contains significant quantities of pentoses. However, the use of pentoses (C5) in the cultivation of microalgae is still not widely studied and only recently the first metabolic pathway for pentose absorption in microalgae was proposed, so the numbers of works that exploring this subject is still very low...
December 18, 2017: Environmental Technology
Bianca Ferrazzo Naspolini, Antonio Carlos de Oliveira Machado, Walter Barreiro Cravo Junior, Denise Maria Guimarães Freire, Magali Christe Cammarota
Vinasse, a residue from bioethanol production containing high organic matter concentration, was used as substrate in submerged fermentation of Pseudomonas aeruginosa PA1 for biosurfactant production. About 2.7 g/L of rhamnolipids was obtained, with surface tension of 29.2 mN/m and critical micelle concentration of 80.3 mg/L. After separation of rhamnolipid and biomass, residual fermentation media were submitted to anaerobic biodegradation in mesophilic conditions. The residual medium derived from fermentation with vinasse diluted to 1 : 1, without addition of nitrogen, C : N 21, and for 168 h, led to 63...
2017: BioMed Research International
Behzad Satari, Keikhosro Karimi
Mucoralean fungi are suitable microorganisms for the sustainable production of food, fodder, and fuels from inexpensive natural resources. Ethanol-producing Mucorales are particularly advantageous for second-generation ethanol production in comparison to the conventional ethanolic yeasts and bacteria. They are able to ferment a wide range of sugars to a range of valuable products, while they are typically resistance against the inhibitors available in different substrates, including untreated lignocellulosic hydrolysates...
December 15, 2017: Applied Microbiology and Biotechnology
Kittipong Rattanaporn, Prapakorn Tantayotai, Theerawut Phusantisampan, Peerapong Pornwongthong, Malinee Sriariyanun
Effective lignocellulosic biomass saccharification is one of the crucial requirements of biofuel production via fermentation process. Organic acid pretreatments have been gained much interests as one of the high potential methods for promoting enzymatic saccharification of lignocellulosic materials due to their lower hazardous properties and lower production of inhibitory by-products of fermentation than typical chemical pretreatment methods. In this study, three organic acids, including acetic acid, oxalic acid, and citric acid, were examined for improvement of enzymatic saccharification and bioethanol production from oil palm trunk biomass...
December 16, 2017: Bioprocess and Biosystems Engineering
Jeroen G Nijland, Hyun Yong Shin, Paul P de Waal, Paul Klaassen, Arnold J M Driessen
AIMS: Optimizing D-xylose transport in Saccharomyces cerevisiae is essential for efficient bioethanol production from cellulosic materials. We have used a gene shuffling approach of hexose (Hxt) transporters in order to increase the affinity for D-xylose. METHODS AND RESULTS: Various libraries were transformed to a hexose transporter deletion strain and shuffled genes were selected via growth on low concentrations of D-xylose. This screening yielded two homologous fusion proteins (fusion 9,4 and 9,6), both consisting of the major central part of Hxt2 and various smaller parts of other Hxt proteins...
December 14, 2017: Journal of Applied Microbiology
Cristhyane M Freita, Lidyane A Freita, Leticia F Tralli, Aline F Silva, Franciele Q Mendes, Vitor Teixeira, Marcia J R Mutton
One of the most important steps is to clarify the juice, which are added synthetic polymer acrylamide base, aiming the fast settling of impurities present in the juice. However, this input is expensive and may have carcinogenic and neurotoxic actions to humans. The search for new natural flocculants that have similarity with the commercial product is of great value. A bioextract that may be promising and has coagulant action is the Moringa oleifera Lam. In this context, the objective of the research was to evaluate the consequences of the use of moringa seed extracts and various concentrations of commercial polymer, such as sedimentation aids in clarifying sugarcane juice in the ethanol production, comparing the efficiency of the bioextract moringa...
December 11, 2017: Anais da Academia Brasileira de Ciências
William P Bewg, Heather D Coleman
Sugarcane bagasse is an abundant source of lignocellulosic material for bioethanol production. Utilisation of bagasse for biofuel production would be environmentally and economically beneficial, but the recalcitrance of lignin continues to provide a challenge. Further understanding of lignin production in specific cultivars will provide a basis for modification of genomes for the production of phenotypes with improved processing characteristics. Here we evaluated the expression profile of lignin biosynthetic genes and the cell wall composition along a developmental gradient in KQ228 sugarcane...
2017: PeerJ
Katarzyna Szambelan, Jacek Nowak, Jakub Frankowski, Artur Szwengiel, Henryk Jeleń, Henryk Burczyk
The aim of this work was to study the potential of sorghum crop cultivated in European climate as an energy material. The investigation showed strong interaction between the fermentation method and the sorghum cultivar. It was also noted that the cultivar with the highest grain yield showed the highest yield of ethanol per hectare, achieving 1269 L/ha in SHF (separate hydrolysis and fermentation) and 1248 L/ha in SSF (simultaneous saccharification and fermentation). Chromatographic analysis of raw spirits showed that smaller amounts of impurities are formed in the SSF process than in the SHF process...
December 6, 2017: Bioresource Technology
Yubin Ma, Ming Zhu, Changjiang Yu, Yu Wang, Yu Liu, Mingliang Li, Youde Sun, Jinshan Zhao, Gongke Zhou
Duckweed has been considered as a promising feedstock for bioethanol production due to its high biomass and starch production. Selection of duckweed strain with high starch accumulation is an essential process in application of duckweeds for bioethanol production. Geographic differentiation had great influence on genetic diversity of duckweed. Biomass production, starch content and starch production of 20 Lemna aequinoctialis and Spirodela polyrhiza geographically isolated strains were calculated to evaluate their potential for bioethanol production...
December 9, 2017: Plant Biology
Raymond M Trevorah, Tien Huynh, Tony Vancov, Maazuza Z Othman
Optimisation of conditions for gamma-valerolactone (GVL) pretreatment of Australian eucalyptus sawdust for high cellulose biomass and bioethanol production was demonstrated. Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design. Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0...
November 28, 2017: Bioresource Technology
Chadaporn Krutpijit, Bunjerd Jongsomjit
Montmorillonite clay (MMT) is one of materials that can be "green material" due to its environmental safety. In this work, acid-activated MMT catalysts were prepared for the dehydration reaction of ethanol. To be the green process, the reaction with bioethanol was also studied. Ethanol concentrations in feed were varied in the range of 10-99.95 wt%. Moreover, the concentrations of hydrochloric acid activated MMT were investigated in range of 0.05-4 M. From the experiment, it reveals that different acid concentrations to activate MMT affect the catalytic activity of catalysts...
2017: Journal of Oleo Science
Matthias Frommhagen, Adrie H Westphal, Roelant Hilgers, Martijn J Koetsier, Sandra W A Hinz, Jaap Visser, Harry Gruppen, Willem J H van Berkel, Mirjam A Kabel
Lytic polysaccharide monooxygenases (LPMOs) have recently been shown to significantly enhance the degradation of recalcitrant polysaccharides and are of interest for the production of biochemicals and bioethanol from plant biomass. The copper-containing LPMOs utilize electrons, provided by reducing agents, to oxidatively cleave polysaccharides. Here, we report the development of a β-glucosidase-assisted method to quantify the release of C1-oxidized gluco-oligosaccharides from cellulose by two C1-oxidizing LPMOs from Myceliophthora thermophila C1...
December 2, 2017: Applied Microbiology and Biotechnology
Ja Kyong Ko, Sun-Mi Lee
Cellulosic fuels are expected to have great potential industrial applications in the near future, but they still face technical challenges to become cost-competitive fuels, thus presenting many opportunities for improvement. The economical production of viable biofuels requires metabolic engineering of microbial platforms to convert cellulosic biomass into biofuels with high titers and yields. Fortunately, integrating traditional and novel engineering strategies with advanced engineering toolboxes has allowed the development of more robust microbial platforms, thus expanding substrate ranges...
November 27, 2017: Current Opinion in Biotechnology
Yong Chen, Li-Ping Sun, Zhi-Hui Liu, Greg Martin, Zheng Sun
Managing waste is an increasing problem globally. Microalgae have the potential to help remove contaminants from a range of waste streams and convert them into useful biomass. This article presents a critical review of recent technological developments in the production of chemicals and other materials from microalgae grown using different types of waste. A range of novel approaches are examined for efficiently capturing CO2 in flue gas via photosynthetic microalgal cultivation. Strategies for using microalgae to assimilate nitrogen, organic carbon, phosphorus, and metal ions from wastewater are considered in relation to modes of production...
November 27, 2017: Topics in Current Chemistry (Journal)
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