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https://www.readbyqxmd.com/read/27905050/transcriptional-analysis-of-genes-encoding-%C3%AE-glucosidase-of-schizophyllum-commune-kuc9397-under-optimal-conditions
#1
Young Min Lee, Hanbyul Lee, Young Mok Heo, Hwanhwi Lee, Joo-Hyun Hong, Jae-Jin Kim
The present study was conducted to determine the gene responsible for beta-glucosidase (BGL) production and to generate a full-length complementary DNA (cDNA) of one of the putative BGL genes, which showed a significant expression level when Schizophyllum commune KUC9397 was grown in optimized medium. The relative expression levels of seven genes encoding BGL of S. commune KUC9397 were determined with real-time quantitative reverse transcription PCR in cellulose-containing optimized medium (OM) compared to glucose-containing basal medium (BM)...
November 30, 2016: Folia Microbiologica
https://www.readbyqxmd.com/read/27900691/screening-for-cellulase-encoding-clones-in-metagenomic-libraries
#2
Nele Ilmberger, Wolfgang R Streit
For modern biotechnology there is a steady need to identify novel enzymes. In biotechnological applications, however, enzymes often must function under extreme and nonnatural conditions (i.e., in the presence of solvents, high temperature and/or at extreme pH values). Cellulases have many industrial applications from the generation of bioethanol, a realistic long-term energy source, to the finishing of textiles. These industrial processes require cellulolytic activity under a wide range of pH, temperature, and ionic conditions, and they are usually carried out by mixtures of cellulases...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27900528/the-thermophilic-biomass-degrading-fungus-thielavia-terrestris-co3bag1-produces-a-hyperthermophilic-and-thermostable-%C3%AE-1-4-xylanase-with-exo-and-endo-activity
#3
Yolanda García-Huante, Maribel Cayetano-Cruz, Alejandro Santiago-Hernández, Claudia Cano-Ramírez, Rodolfo Marsch-Moreno, Jorge E Campos, Guillermo Aguilar-Osorio, Claudia G Benitez-Cardoza, Sergio Trejo-Estrada, María Eugenia Hidalgo-Lara
A hyperthermophilic and thermostable xylanase of 82 kDa (TtXynA) was purified from the culture supernatant of T. terrestris Co3Bag1, grown on carboxymethyl cellulose (CMC), and characterized biochemically. TtXynA showed optimal xylanolytic activity at pH 5.5 and at 85 °C, and retained more than 90% of its activity at a broad pH range (4.5-10). The enzyme is highly thermostable with a half-life of 23.1 days at 65 °C, and active in the presence of several metal ions. Circular dichroism spectra strongly suggest the enzyme gains secondary structures when temperature increases...
November 29, 2016: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/27898315/single-step-single-organism-bioethanol-production-and-bioconversion-of-lignocellulose-waste-materials-by-phlebioid-fungal-species
#4
Hans Mattila, Jaana Kuuskeri, Taina Lundell
Ethanol production from non-pretreated lignocellulose was carried out in a consolidated bioprocess with wood-decay fungi of phlebioid Polyporales. Ethanol production was attempted on glucose, spruce wood sawdust and waste core board. Substantial quantities of ethanol were achieved, and isolate Phlebia radiata 0043 produced 5.9g/L of ethanol reaching the yield of 10.4% ethanol from core board lignocellulose substrate. Acidic initial culture conditions (pH 3) induced ethanol fermentation compared to the more neutral environment...
November 22, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27897278/separation-of-water-ethanol-solutions-with-carbon-nanotubes-and-electric-fields
#5
Winarto, Daisuke Takaiwa, Eiji Yamamoto, Kenji Yasuoka
Bioethanol has been used as an alternative energy source for transportation vehicles to reduce the use of fossil fuels. The separation of water-ethanol solutions from fermentation processes is still an important issue in the production of anhydrous ethanol. Using molecular dynamics simulations, we investigate the effect of axial electric fields on the separation of water-ethanol solutions with carbon nanotubes (CNTs). In the absence of an electric field, CNT-ethanol van der Waals interactions allow ethanol to fill the CNTs in preference to water, i...
November 29, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27894041/valorization-of-exhausted-sugar-beet-cossettes-by-successive-hydrolysis-and-two-fermentations-for-the-production-of-bio-products
#6
A B Díaz, C Marzo, I Caro, I de Ory, A Blandino
Exhausted sugar beet cossettes (ESBC) show an enormous potential as a source of sugars for the production of bio-products. Enzyme hydrolysis with the combined effect of mainly cellulases, xylanases and pectinases, turned out to be very efficient, obtaining almost double the concentration of sugars measured with the sole action of Celluclast® and β-glucosidase, and increasing 5 times the hydrolysis rate. As the sole pretreatment, ESBC soaked in the hydrolysis buffer were autoclaved, avoiding the application of severe conventional biomass pretreatments...
November 19, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27890094/functional-expression-production-and-biochemical-characterization-of-a-laccase-using-yeast-surface-display-technology
#7
Brandt Bertrand, María R Trejo-Hernández, Daniel Morales-Guzmán, Luis Caspeta, Ramón Suárez Rodríguez, Fernando Martínez-Morales
A Trametes versicolor laccase was functionally expressed on the membrane surface of Saccharomyces cerevisiae EBY100. Laccase expression was increased 6.57-fold by medium optimization and surpassed production by the native strain. Maximal laccase and biomass production reached 19 735 ± 1719 Ug(-1) and 6.22 ± 0.53 gL(-1) respectively, after 2 d of culture. Optimum oxidization of all substrates by laccase was observed at pH 3. Laccase showed high affinity towards substrates used with Km (mM) and Vmax (μmol min(-1)) values of 0...
December 2016: Fungal Biology
https://www.readbyqxmd.com/read/27888730/production-and-purification-of-amylolytic-enzymes-for-saccharification-of-microalgal-biomass
#8
Éllen Francine Rodrigues, Aline Matuella Moreira Ficanha, Rogério Marcos Dallago, Helen Treichel, Christian Oliveira Reinehr, Tainara Paula Machado, Greice Borges Nunes, Luciane Maria Colla
The aim of this study was the production of amylolytic enzymes by solid state or submerged fermentations (SSF or SF, respectively), followed by purification using chemical process or microfiltration and immobilization of purified enzymes in a polyurethane support. The free and immobilized enzymes obtained were used to evaluate enzymatic hydrolysis of the polysaccharides of Spirulina. Microfiltration of the crude extracts resulted in an increase in their specific activity and thermal stability at 40°C and 50°C for 24h, as compared to extracts obtained by SSF and SF...
November 16, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27878508/calcium-supplementation-abates-the-inhibition-effects-of-acetic-acid-on-saccharomyces-cerevisiae
#9
Hongwei Zhao, Jingyuan Li, Jiming Wang, Xin Xu, Mo Xian, Huizhou Liu, Haibo Zhang
The toxic level of acetic acid could be released during the pretreatment of lignocellulosic biomass, and an economical method was reported to minimize the acidic stress on the fermentation of Saccharomyces cerevisiae by cation supplementation. A dose-dependent protection of Ca(2+) was monitored, and the optimal concentration of Ca(2+) was 8 mM under 4.5 g/L acetic acid stress. The activities of catalase and superoxide dismutase of yeast cells supplemented with optimal Ca(2+) increased by 18.6 and 27.3 %, respectively, coupling with an obvious decrease of reactive oxygen species content...
November 22, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27875964/bioengineering-for-microbial-inulinases-trends-and-applications
#10
Puneet Kumar Singh, Vishal Kumar, Ruby Yadav, Pratyoosh Shukla
Inulinase has attracted attention due to their number of applications in various industries viz. pharmaceuticals, food and bioethanol. Enzymes due to their unique properties and enormous power of catalysis at very wide range of temperature are always in demand in industries. There are certain techniques which are employed to improve the productivity of enzymes as well as enhancing their catalytic activity. Modeling of structure of inulinase will provide an overview of the catalytic domain and help in improvising catalytic potential...
November 22, 2016: Current Protein & Peptide Science
https://www.readbyqxmd.com/read/27873164/bacterial-signals-n-acyl-homoserine-lactones-induce-the-changes-of-morphology-and-ethanol-tolerance-in-saccharomyces-cerevisiae
#11
Ge Ren, Anzhou Ma, Weifeng Liu, Xuliang Zhuang, Guoqiang Zhuang
The bacterial quorum sensing signals N-acyl homoserine lactone (AHL) signals are able to regulate a diverse array of physiological activities, such as symbiosis, virulence and biofilm formation, depending on population density. Recently, it has been discovered that the bacterial quorum sensing (QS) signal molecules can induce extensive response of higher eukaryotes including plants and mammalian cells. However, little is known about the response of fungi reacting to these bacterial signals. Here we showed that Saccharomyces cerevisiae, as an ancient eukaryote and widely used for alcoholic beverage and bioethanol production, exposed to short-chain 3-OC6-HSL and long-chain C12-HSL appeared obvious changes in morphology and ethanol tolerance...
December 2016: AMB Express
https://www.readbyqxmd.com/read/27872659/whole-genome-de-novo-sequencing-combined-with-rna-seq-analysis-reveals-unique-genome-and-physiological-features-of-the-amylolytic-yeast-saccharomycopsis-fibuligera-and-its-interspecies-hybrid
#12
Jin Ho Choo, Chang Pyo Hong, Jae Yun Lim, Jeong-Ah Seo, Young-Suk Kim, Dong Wook Lee, Sin-Gi Park, Gir Won Lee, Emily Carroll, Yin-Won Lee, Hyun Ah Kang
BACKGROUND: Genomic studies on fungal species with hydrolytic activity have gained increased attention due to their great biotechnological potential for biomass-based biofuel production. The amylolytic yeast Saccharomycopsis fibuligera has served as a good source of enzymes and genes involved in saccharification. Despite its long history of use in food fermentation and bioethanol production, very little is known about the basic physiology and genomic features of S. fibuligera. RESULTS: We performed whole-genome (WG) de novo sequencing and complete assembly of S...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27866804/evaluation-of-ultrasound-assisted-potassium-permanganate-pre-treatment-of-spent-coffee-waste
#13
Rajeev Ravindran, Swarna Jaiswal, Nissreen Abu-Ghannam, Amit K Jaiswal
In the present study, novel pre-treatment for spent coffee waste (SCW) has been proposed which utilises the superior oxidising capacity of alkaline KMnO4 assisted by ultra-sonication. The pre-treatment was conducted for different exposure times (10, 20, 30 and 40min) using different concentrations of KMnO4 (1, 2, 3, 4, 5%w/v) at room temperature with solid/liquid ratio of 1:10. Pretreating SCW with 4% KMnO4 and exposing it to ultrasound for 20min resulted in 98% cellulose recovery and a maximum lignin removal of 46%...
November 9, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27865731/physicochemical-characterization-modelling-and-optimization-of-ultrasono-assisted-acid-pretreatment-of-two-pennisetum-sp-using-taguchi-and-artificial-neural-networking-for-enhanced-delignification
#14
Sonali Mohapatra, Snigdha Jyotsna Dandapat, Hrudayanath Thatoi
Acid as well as ultrasono-assisted acid pretreatment of lignocellulosic biomass of two Pennisetum sp.; Denanath grass (DG) and Hybrid Napier grass (HNG) have been investigated for enhanced delignification and maximum exposure of cellulose for production of bioethanol. Screening of pretreatment with different acids such as H2SO4, HCl, H3PO4 and H2NO3 were optimized for different temperature, soaking time and acid concentrations using Taguchi orthogonal array and the data obtained were statistically validated using artificial neural networking...
November 16, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/27856231/bioprospecting-thermotolerant-ethanologenic-yeasts-for-simultaneous-saccharification-and-fermentation-from-diverse-environments
#15
Jairam Choudhary, Surender Singh, Lata Nain
Lignocellulosic biomass, a promising renewable energy source, can be used for the production of second generation bioethanol. Simultaneous saccharification and fermentation (SSF), the process which alleviates the problem of separate hydrolysis and fermentation (SHF), requires thermotolerant ethanologenic yeast for bioethanol production. Therefore, ten yeast strains isolated from diverse sources, belonging to various genera like Saccharomyces, Candida, Pichia and Wickerhamomyces were evaluated for their thermotolerance, sugar utilization pattern, inhibitor tolerance and ethanol production potential with glucose, xylose and alkali pretreated paddy straw...
November 14, 2016: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/27852874/high-speed-scanning-for-the-quantitative-evaluation-of-glycogen-concentration-in-bioethanol-feedstock-synechocystis-sp-pcc6803-using-a-near-infrared-hyperspectral-imaging-system-with-a-new-near-infrared-spectral-camera
#16
Mika Ishigaki, Akihito Nakanishi, Tomohisa Hasunuma, Akihiko Kondo, Tetsu Morishima, Toshiaki Okuno, Yukihiro Ozaki
In the present study, the high-speed quantitative evaluation of glycogen concentration accumulated in bioethanol feedstock Synechocystis sp. PCC6803 was performed using a near-infrared (NIR) imaging system with a hyperspectral NIR spectral camera named Compovision. The NIR imaging system has a feature for high-speed and wide area monitoring and the two-dimensional scanning speed is almost 100 times faster than the general NIR imaging systems for the same pixel size. For the quantitative analysis of glycogen concentration, partial least squares regression (PLSR) and moving window PLSR (MWPLSR) were performed with the information of glycogen concentration measured by high performance liquid chromatography (HPLC) and the calibration curves for the concentration within the Synechocystis sp...
November 16, 2016: Applied Spectroscopy
https://www.readbyqxmd.com/read/27833653/valorization-of-lignin-and-cellulose-in-acid-steam-exploded-corn-stover-by-a-moderate-alkaline-ethanol-post-treatment-based-on-an-integrated-biorefinery-concept
#17
Sheng Yang, Yue Zhang, Wen Yue, Wei Wang, Yun-Yan Wang, Tong-Qi Yuan, Run-Cang Sun
BACKGROUND: Due to the unsustainable consumption of fossil resources, great efforts have been made to convert lignocellulose into bioethanol and commodity organic compounds through biological methods. The conversion of cellulose is impeded by the compactness of plant cell wall matrix and crystalline structure of the native cellulose. Therefore, appropriate pretreatment and even post-treatment are indispensable to overcome this problem. Additionally, an adequate utilization of coproduct lignin will be important for improving the economic viability of modern biorefinery industries...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27816352/economic-and-environmental-assessment-of-cellulosic-ethanol-production-scenarios-annexed-to-a-typical-sugar-mill
#18
Mohsen Ali Mandegari, Somayeh Farzad, Johann F Görgens
In this work different biorefinery scenarios were investigated, concerning the co-production of bioethanol and electricity from available lignocellulose at a typical sugar mill, as possible extensions to the current combustion of bagasse for steam and electricity production and burning trash on-filed. In scenario 1, the whole bagasse and brown leaves is utilized in a biorefinery and coal is burnt in the existing inefficient sugar mill boiler. Scenario 2 & 3 are assumed with a new centralized CHP unit without/with coal co-combustion, respectively...
October 28, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27800014/dry-grind-processing-using-amylase-corn-and-superior-yeast-to-reduce-the-exogenous-enzyme-requirements-in-bioethanol-production
#19
Deepak Kumar, Vijay Singh
BACKGROUND: Conventional corn dry-grind ethanol production process requires exogenous alpha and glucoamylases enzymes to breakdown starch into glucose, which is fermented to ethanol by yeast. This study evaluates the potential use of new genetically engineered corn and yeast, which can eliminate or minimize the use of these external enzymes, improve the economics and process efficiencies, and simplify the process. An approach of in situ ethanol removal during fermentation was also investigated for its potential to improve the efficiency of high-solid fermentation, which can significantly reduce the downstream ethanol and co-product recovery cost...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27777968/adaptability-and-stability-study-of-selected-sweet-sorghum-genotypes-for-ethanol-production-under-different-environments-using-ammi-analysis-and-gge-biplots
#20
Justice Kipkorir Rono, Erick Kimutai Cheruiyot, Jacktone Odongo Othira, Virginia Wanjiku Njuguna, Joseph Kinyoro Macharia, James Owuoche, Moses Oyier, Alex Machio Kange
The genotype and environment interaction influences the selection criteria of sorghum (Sorghum bicolor) genotypes. Eight sweet sorghum genotypes were evaluated at five different locations in two growing seasons of 2014. The aim was to determine the interaction between genotype and environment on cane, juice, and ethanol yield and to identify best genotypes for bioethanol production in Kenya. The experiments were conducted in a randomized complete block design replicated three times. Sorghum canes were harvested at hard dough stage of grain development and passed through rollers to obtain juice that was then fermented to obtain ethanol...
2016: TheScientificWorldJournal
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