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Peng-Fei Xia, Guo-Chang Zhang, Berkley Walker, Seung-Oh Seo, Suryang Kwak, Jingjing Liu, Heejin Kim, Donald Ort, Shu-Guang Wang, Yong-Su Jin
Global climate change caused by the emission of anthropogenic greenhouse gasses (GHGs) is a grand challenge to humanity. To alleviate the trend, the consumption of fossil fuels needs to be largely reduced and alternative energy technologies capable of controlling GHG emissions are anticipated. In this study, we introduced a synthetic reductive pentose phosphate pathway (rPPP) into a xylose-fermenting Saccharomyces cerevisiae strain SR8 to achieve simultaneous lignocellulosic bioethanol production and carbon dioxide recycling...
October 17, 2016: ACS Synthetic Biology
Ja Kyong Ko, Youngsoon Um, Sun-Mi Lee
The efficient fermentation of lignocellulosic hydrolysates in the presence of inhibitors is highly desirable for bioethanol production. Among the inhibitors, acetic acid released during the pretreatment of lignocellulose negatively affects the fermentation performance of biofuel producing organisms. In this study, we evaluated the inhibitory effects of acetic acid on glucose and xylose fermentation by a high performance engineered strain of xylose utilizing Saccharomyces cerevisiae, SXA-R2P-E, harboring a xylose isomerase based pathway...
October 6, 2016: Bioresource Technology
Joanna Berłowska, Katarzyna Pielech-Przybylska, Maria Balcerek, Urszula Dziekońska-Kubczak, Piotr Patelski, Piotr Dziugan, Dorota Kręgiel
Sugar beet pulp, a byproduct of sugar beet processing, can be used as a feedstock in second-generation ethanol production. The objective of this study was to investigate the effects of pretreatment, of the dosage of cellulase and hemicellulase enzyme preparations used, and of aeration on the release of fermentable sugars and ethanol yield during simultaneous saccharification and fermentation (SSF) of sugar beet pulp-based worts. Pressure-thermal pretreatment was applied to sugar beet pulp suspended in 2% w/w sulphuric acid solution at a ratio providing 12% dry matter...
2016: BioMed Research International
Maria Muñoz, Norbert V Heeb, Regula Haag, Peter Honegger, Kerstin Zeyer, Joachim Mohn, Pierre Comte, Jan Czerwinski
Bioethanol as an alternative fuel is widely used as a substitute for gasoline, also in gasoline direct injection (GDI) vehicles, which are quickly replacing traditional port-fuel injection (PFI) vehicles. Better fuel efficiency and increased engine power are reported advantages of GDI-vehicles. But increased emissions of soot-like nanoparticles are also associated with the GDI-technology with yet unknown health impact. In this study, we compare emissions of a flex-fuel Euro-5 GDI-vehicle operated with gasoline (E0) and two ethanol/gasoline blends (E10 and E85) under transient and steady driving conditions and report effects on particle, polycyclic aromatic hydrocarbon (PAH), alkyl- and nitro-PAH emissions and assess the genotoxic potential of them...
October 7, 2016: Environmental Science & Technology
Mariana B G Latarullo, Eveline Q P Tavares, Gabriel P Maldonado, Débora C C Leite, Marcos S Buckeridge
The precise disassembly of the extracellular matrix of some plant species used as feedstocks for bioenergy production continues to be a major barrier to reach reasonable cost effective bioethanol production. One solution has been the use of pretreatments, which can be effective, but increase even more the cost of processing and also lead to loss of cell wall materials that could otherwise be used in industry. Although pectins are known to account for a relatively low proportion of walls of grasses, their role in recalcitrance to hydrolysis has been shown to be important...
2016: Frontiers in Plant Science
Raúl N Comelli, Lisandro G Seluy, Miguel A Isla
This work focuses on the performance of ten commercial Saccharomyces yeast strains in the batch alcoholic fermentation of sugars contained in selected industrial wastewaters from the soft drink industry. Fermentation has been applied successfully to treat these effluents prior to their disposal. Although many strains were investigated, similar behaviour was observed between all of the Saccharomyces strains tested. When media were inoculated with 2gL(-1) of yeast, all strains were able to completely consume the available sugars in less than 14h...
October 1, 2016: New Biotechnology
Kentaro Inokuma, Tomohisa Hasunuma, Akihiko Kondo
N-acetyl-D-glucosamine (GlcNAc) is the building block of chitin, which is one of the most abundant renewable resources in nature after cellulose. Therefore, a microorganism that can utilize GlcNAc is necessary for chitin-based biorefinery. In this study, we report on the screening and characterization of yeast strains for bioethanol production from GlcNAc. We demonstrate that Scheffersomyces (Pichia) stipitis strains can use GlcNAc as the sole carbon source and produce ethanol. S. stipitis NBRC1687, 10007, and 10063 strains consumed most of the 50 g/L GlcNAc provided, and produced 14...
December 2016: AMB Express
Muthusamy Sanjivkumar, TamilSelvan Silambarasan, Arunachalam Palavesam, Grasian Immanuel
The present study was undertaken to evaluate the xylanolytic properties of an actinobacterium Streptomyces olivaceus (MSU3) isolated from the sediment sample of mangrove environment. It showed highest xylanase activity on initial screening in Breg's mineral salts medium supplemented with 0.5% xylan. Further the organism expressed maximum xylanase production at optimized culture conditions of pH 7, temperature 30 °C with 2.5% of inoculum size at 72 h of incubation, the nutrient sources like sucrose (2%) and yeast extract (3%) have observed as best carbon and nitrogen sources respectively...
September 28, 2016: Protein Expression and Purification
C Sambusiti, F Monlau, N Antoniou, A Zabaniotou, A Barakat
Pyrolysis is a sustainable pathway to transform renewable biomasses into both biofuels and advanced carbonaceous materials (i.e. pyrochar) which can be used as adsorbent of furan compounds. In particular, the aim of this study was to: i) evaluate the effect of vibro-ball milling on physical characteristics of pyrochar and its consequent performance on solely detoxification of a synthetic medium, containing furans and soluble sugars; ii) study the simultaneous detoxification and bioethanol fermentation, by adding activated pyrochar into fermentation medium...
December 1, 2016: Journal of Environmental Management
Lili Sheng, Ying Zhang, Nigel P Minton
The industrially important thermophile Geobacillus thermoglucosidasius has the potential to produce chemicals and fuels from biomass-derived sugar feedstocks. Here, we present the genome sequence of strain NCIMB 11955, the progenitor of an ethanologenic industrial strain, revealing 11 single-nucleotide polymorphisms and 2 indels compared to strain DSM 2542 and two novel plasmids.
September 29, 2016: Genome Announcements
Olivia A Thompson, Gary M Hawkins, Steven W Gorsich, Joy Doran-Peterson
BACKGROUND: Lignocellulosic biomass continues to be investigated as a viable source for bioethanol production. However, the pretreatment process generates inhibitory compounds that impair the growth and fermentation performance of microorganisms such as Saccharomyces cerevisiae. Pinewood specifically has been shown to be challenging in obtaining industrially relevant ethanol titers. An industrial S. cerevisiae strain was subjected to directed evolution and adaptation in pretreated pine biomass and resultant strains, GHP1 and GHP4, exhibited improved growth and fermentative ability on pretreated pine in the presence of related inhibitory compounds...
2016: Biotechnology for Biofuels
Kaumeel Chokshi, Imran Pancha, Arup Ghosh, Sandhya Mishra
Dairy wastewater collected from local dairy industry was used as a growth media (without any pre-treatment) for the cultivation of microalgae Acutodesmus dimorphus. The level of COD reduced over 90% (from 2593.33±277.37 to 215±7.07mg/L) after 4days of cultivation; whereas, ammoniacal nitrogen was consumed completely (277.4±10.75mg/L) after 6days of cultivation. Dry biomass of 840 and 790mg/L was observed after 4 and 8days of cultivation, respectively, which is about 5-6 times more than that of BG-11 grown culture (149mg/L after 8days)...
December 2016: Bioresource Technology
Sravanthi Koti, Sai Prashanthi Govumoni, Jahnavi Gentela, L Venkateswar Rao
The main aim of the present study was to mutate yeast strains, Pichia stipitis NCIM 3498 and Candida shehatae NCIM 3501 and assess the mutant's ability to utilize, ferment wheat straw hemicellulose with enhanced ethanol yield. The organisms were subjected to random mutagenesis using physical (ultraviolet radiation) and chemical (ethidium bromide) mutagens. The mutant and wild strains were used to ferment the hemicellulosic hydrolysates of wheat straw obtained by 2 % dilute sulphuric acid and enzymatic hydrolysis by crude xylanase separately...
2016: SpringerPlus
Marc Sugnaux, Manuel Happe, Christian Pierre Cachelin, Olivier Gloriod, Gérald Huguenin, Maxime Blatter, Fabian Fischer
Ethanol, electricity, hydrogen and methane were produced in a two stage bioethanol refinery setup based on a 10L microbial fuel cell (MFC) and a 33L microbial electrolysis cell (MEC). The MFC was a triple stack for ethanol and electricity co-generation. The stack configuration produced more ethanol with faster glucose consumption the higher the stack potential. Under electrolytic conditions ethanol productivity outperformed standard conditions and reached 96.3% of the theoretically best case. At lower external loads currents and working potentials oscillated in a self-synchronized manner over all three MFC units in the stack...
December 2016: Bioresource Technology
B Cassells, K Karhumaa, V Sànchez I Nogué, G Lidén
Wheat straw is one of the main agricultural residues of interest for bioethanol production. This work examines conversion of steam-pretreated wheat straw (using SO2 as a catalyst) in a hybrid process consisting of a short enzymatic prehydrolysis step and a subsequent simultaneous saccharification and fermentation (SSF) step with a xylose-fermenting strain of Saccharomyces cerevisiae. A successful process requires a balanced design of reaction time and temperature in the prehydrolysis step and yeast inoculum size and temperature in the SSF step...
September 8, 2016: Applied Biochemistry and Biotechnology
Carlos Eduardo De Farias Silva, Eleonora Sforza, Alberto Bertucco
Synechococcus PCC 7002 is an interesting species in view of industrial production of carbohydrates. The cultivation performances of this species are strongly affected by the pH of the medium, which also influences the carbohydrate accumulation. In this work, different methods of pH control were analyzed, in order to obtain a higher production of both Synechococcus biomass and carbohydrates. To better understand the influence of pH on growth and carbohydrate productivity, manual and automatic pH regulation in CO2 and bicarbonate system were applied...
September 13, 2016: Applied Biochemistry and Biotechnology
Bruna Medeia Campos, Marcelo Vizona Liberato, Thabata Maria Alvarez, Leticia Maria Zanphorlin, Gabriela Cristina Ematsu, Hernane Barud, Igor Polikarpov, Roberto Ruller, Harry J Gilbert, Ana Carolina de Mattos Zeri, Fabio Marcio Squina
Carbohydrate-binding modules (CBMs) are appended to glycoside hydrolases and can contribute to the degradation of complex recalcitrant substrates such as the plant cell wall. For application in bioethanol production, novel enzymes with high catalytic activity against recalcitrant lignocellulosic material are being explored and developed. In this work, we report the functional and structural study of CBM_E1, discovered through a metagenomics approach, which is the founding member of a novel CBM family, CBMxx...
September 12, 2016: Journal of Biological Chemistry
B S Choudri, Mahad Baawain
This review includes works published in the general scientific literature during 2015 on the production of bioenergy and biofuel from waste residues generated during bioethanol and biodiesel production with a brief overview of current and emerging feedstocks. A section of this review summarizes literature on culturing algae for biofuels including bioreactors and open pond cultivation systems with the utilization of inorganic and organic sources of nutrients. New methods applicable to the mass culture of algae are highlighted...
October 2016: Water Environment Research: a Research Publication of the Water Environment Federation
Rishikesh Shukla, Manoj Kumar, Subhojit Chakraborty, Rishi Gupta, Savindra Kumar, Dinabandhu Sahoo, Ramesh Chander Kuhad
The algal biomass of different species of Gracilaria were collected from coasts of Orissa and Tamil Nadu, India and characterized biochemically. Among various species, G. verrucosa was found to be better in terms of total carbohydrate content (56.65%) and hence selected for further studies. The agar was extracted from algal biomass and the residual pulp was enzymatically hydrolyzed. The optimization of algal pulp hydrolysis for various parameters revealed a maximum sugar release of 75.8mg/ml with 63% saccharification yield...
November 2016: Bioresource Technology
Tao Yuan, Xiekun Li, Shiyuan Xiao, Ying Guo, Weizheng Zhou, Jingliang Xu, Zhenhong Yuan
Nowadays, microalgae are being considered as promising raw material for bioethanol production. In this work, three process variables during liquid hot water (LHW) pretreatment prior to enzymatic hydrolysis by response surface methodology on Scenedesmus sp. WZKMT were investigated to enhance glucose recovery. Results indicated that the order of significance for three parameters was temperature>solid-to-liquid ratio>time. The optimal condition was 1:13 (w/v), 147°C and 40min. The concentration and recovery of glucose under this condition were 14...
November 2016: Bioresource Technology
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