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Anderson Albino Gomes, Elisa Pazinatto Telli, Luiz Claudio Miletti, Everton Skoronski, Marcos Gomes Ghislandi, Gustavo Felippe da Silva, Maria de Lourdes Borba Magalhães
Optimization of cellulose enzymatic hydrolysis is crucial for cost effective bioethanol production from lignocellulosic biomass. Enzyme immobilization in solid support allows enzyme recycling for reuse, lowering hydrolysis costs. Graphene is a nanomaterial isolated in 2004, which possesses exceptional properties for biomolecule immobilization. This study evaluates the potential for β-glucosidase recycling by immobilization on graphene nanosheets. Data reported here demonstrated that graphene immobilized β-glucosidase can be recycled for at least eight cycles...
June 21, 2017: Biotechnology and Applied Biochemistry
Srisakul Trakarnpaiboon, Nantana Srisuk, Kuakoon Piyachomkwan, Kenji Sakai, Vichien Kitpreechavanich
In the present study, solid state fermentation for the production of raw starch degrading enzyme was investigated by thermotolerant Rhizopus microsporus TISTR 3531 using a combination of agro-industrial wastes as substrates. The obtained crude enzyme was applied for hydrolysis of raw cassava starch and chips at low temperature and subjected to non-sterile ethanol production using raw cassava chips. The agro-industrial wastes ratio was optimized using a simplex axial mixture design. The results showed that the substrate mixture consisting of rice bran: corncob: cassava bagasse at 8 g: 10 g: 2 g yielded the highest enzyme production of 201...
June 21, 2017: Preparative Biochemistry & Biotechnology
Rhulani Makhuvele, Ignatious Ncube, Elbert Lukas Jansen van Rensburg, Daniël Coenrad La Grange
Producing biofuels such as ethanol from non-food plant material has the potential to meet transportation fuel requirements in many African countries without impacting directly on food security. The current shortcomings in biomass processing are inefficient fermentation of plant sugars, such as xylose, especially at high temperatures, lack of fermenting microbes that are able to resist inhibitors associated with pre-treated plant material and lack of effective lignocellulolytic enzymes for complete hydrolysis of plant polysaccharides...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
Bin Chao, Ruiliang Liu, Xueling Zhang, Xu Zhang, Tianwei Tan
The bioethanol production from a novel non-grain feedstock, acorn starch, was studied in this work. The inhibition of tannin in strain growth was investigated, and the effect of tannin was negligible when the tannin concentration was lower than 1g/L in medium. Therefore, the extraction of tannin was performed using 40% (v/v) ethanol-water solution as the solvent for three times under the conditions of solid/liquid ratio 1:20, 60°C, 3h, by which more than 80% of tannin in acorn was extracted and the content of tannin in acorn decreased from 7...
June 8, 2017: Bioresource Technology
Nassim Belmokhtar, Stéphanie Arnoult, Brigitte Chabbert, Jean-Paul Charpentier, Maryse Brancourt-Hulmel
HIGHLIGHTS Biomass production and cell wall composition are differentially impacted by harvesting year and genotypes, influencing then cellulose conversion in miniaturized assay.Using a high-throughput miniaturized and semi-automated method for performing the pretreatment and saccharification steps at laboratory scale allows for the assessment of these factors on the biomass potential for producing bioethanol before moving to the industrial scale. The large genetic diversity of the perennial grass miscanthus makes it suitable for producing cellulosic ethanol in biorefineries...
2017: Frontiers in Plant Science
Gezahegn T Mihiretu, Malin Brodin, Annie F Chimphango, Karin Øyaas, Bård H Hoff, Johann F Görgens
The viability of single-step microwave-induced pressurized hot water conditions for co-production of xylan-based biopolymers and bioethanol from aspenwood sawdust and sugarcane trash was investigated. Extraction of hemicelluloses was conducted using microwave-assisted pressurized hot water system. The effects of temperature and time on extraction yield and enzymatic digestibility of resulting solids were determined. Temperatures between 170-200°C for aspenwood and 165-195°C for sugarcane trash; retention times between 8-22min for both feedstocks, were selected for optimization purpose...
May 30, 2017: Bioresource Technology
Anish Ghimire, Gopalakrishnan Kumar, Periyasamy Sivagurunathan, Sutha Shobana, Ganesh D Saratale, Hyun Woo Kim, Vincenzo Luongo, Giovanni Esposito, Raul Munoz
The interest in microalgae for wastewater treatment and liquid bio-fuels production (i.e. biodiesel and bioethanol) is steadily increasing due to the energy demand of the ultra-modern technological world. The associated biomass and by-product residues generated from these processes can be utilized as a feedstock in anaerobic fermentation for the production of gaseous bio-fuels. In this context, dark fermentation coupled with anaerobic digestion can be a potential technology for the production of hydrogen and methane from these residual algal biomasses...
May 29, 2017: Bioresource Technology
Narendra Naik Deshavath, Mood Mohan, Venkata Dasu Veeranki, Vaibhav V Goud, Srinivasa Rao Pinnamaneni, Tamal Benarjee
Conversion of lignocellulosic biomass into monomeric carbohydrates is economically beneficial and suitable for sustainable production of biofuels. Hydrolysis of lignocellulosic biomass using high acid concentration results in decomposition of sugars into fermentative inhibitors. Thus, the main aim of this work was to investigate the optimum hydrolysis conditions for sorghum brown midrib IS11861 biomass to maximize the pentose sugars yield with minimized levels of fermentative inhibitors at low acid concentrations...
June 2017: 3 Biotech
Priya Phadtare, Swati Joshi, T Satyanarayana
Codon adaptation index (CAI) of a 1263bp long endoglucanase encoding gene from the thermophilic mould Myceliopthora thermophile BJA has been improved from 0.44 to 0.76 by in vitro gene synthesis. The codon optimized endoglucanase gene (Mt-egl) has been constitutively expressed in Pichia pastoris under the regulation of GAP promoter. Recombinant endoglucanase (rMt-egl), purified by size exclusion chromatography, has been confirmed to be a monomeric protein of ∼47kDa. rMt-egl is optimally active at pH 10 and 50°C, displaying stability in broad pH and temperature ranges, with a t1/2 of 60 and 15min at 90 and 100°C, respectively...
June 4, 2017: International Journal of Biological Macromolecules
Anindita Dutta, Katherine Brito, Galina Khramstova, Ariel Mueller, Sireesha Chinthala, Donee Alexander, Damilola Adu, Tope Ibigbami, John Olamijulo, Abayomi Odetunde, Kehinde Adigun, Liese Pruitt, Olufunmilayo Olopade, Oladosu Ojengbede, Sarosh Rana, Christopher O Olopade
BACKGROUND: Maternal exposure to ambient air pollution affects placental growth markers. OBJECTIVES: Investigate impact of household air pollution (HAP) on placental growth markers. METHODS: Two groups of pregnant women were identified: firewood/kerosene stove-users (A, n=33) and bioethanol stove-users (B, n=44) that participated in a randomized control trial in Ibadan, Nigeria. A third group of non-smoking and presumed liquefied petroleum gas-using Chicago women (C, n=19) were included in this exploratory pilot to assess for possible differences between similar racial groups...
December 1, 2017: Science of the Total Environment
Amir Mahboubi, Päivi Ylitervo, Wim Doyen, Heleen De Wever, Bart Molenberghs, Mohammad J Taherzadeh
Finding a technological approach that eases the production of lignocellulosic bioethanol has long been considered as a great industrial challenge. In the current study a membrane bioreactor (MBR) set-up using integrated permeate channel (IPC) membrane panels was used to simultaneously ferment pentose and hexose sugars to ethanol in continuous fermentation of high suspended solid wheat straw hydrolysate. The MBR was optimized to flawlessly operated at high SS concentrations of up to 20% without any significant changes in the permeate flux and transmembrane pressure...
May 21, 2017: Bioresource Technology
Ramon Peres Brexó, Anderson de Souza Sant'Ana
Microbial interactions represent important modulatory role in the dynamics of biological processes. During bioethanol production from sugar cane must, the presence of lactic acid bacteria (LAB) and wild yeasts is inevitable as they originate from the raw material and industrial environment. Increasing the concentration of ethanol, organic acids, and other extracellular metabolites in the fermentation must are revealed as wise strategies for survival by certain microorganisms. Despite this, the co-existence of LAB and yeasts in the fermentation vat and production of compounds such as organic acids and other extracellular metabolites result in reduction in the final yield of the bioethanol production process...
June 2, 2017: Critical Reviews in Biotechnology
Bernd Hackauf, Stefan Haffke, Franz Joachim Fromme, Steffen R Roux, Barbara Kusterer, Dörthe Musmann, Andrzej Kilian, Thomas Miedaner
Genetic diversity in elite rye germplasm as well as F 2:3 testcross design enables fast QTL mapping to approach genes controlling grain yield, grain weight, tiller number and heading date in rye hybrids. Winter rye (Secale cereale L.) is a multipurpose cereal crop closely related to wheat, which offers the opportunity for a sustainable production of food and feed and which continues to emerge as a renewable energy source for the production of bioethanol and biomethane. Rye contributes to increase agricultural crop species diversity particularly in Central and Eastern Europe...
May 31, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Samkelo Malgas, Mariska Thoresen, J Susan van Dyk, Brett I Pletschke
Cellulosic ethanol production relies on the biochemical (enzymatic) conversion of lignocellulose to fermentable sugars and ultimately to bioethanol. However, the cost of lignocellulolytic enzymes is a limiting factor in the commercialisation of this technology. This therefore necessitates the optimisation of lignocellulolytic enzyme cocktails through the elucidation of synergistic interactions between enzymes so as to improve lignocellulose hydrolysis and also lower protein loadings in these reactions. However, many factors affect the synergism that occurs between these lignocellulolytic enzymes, such as enzyme ratios, substrate characteristics, substrate loadings, enzyme loadings and time...
August 2017: Enzyme and Microbial Technology
Mohamed A Gomaa, Raeid M M Abed
Fecal waste is an environmental burden that requires proper disposal, which ultimately becomes also an economic burden. Because fecal waste is nutrient-rich and contains a diverse methanogenic community, it has been utilized to produce biomethane via anaerobic digestion. Carbohydrates and lipids in fecal waste could reach up to 50% of the dry weight, which also suggests a potential as a feedstock for bioethanol and biodiesel production. We measured biomethane production from fecal waste of cows, chickens, goats and humans and compared the microbial community composition before and after anaerobic digestion...
May 22, 2017: Journal of Biotechnology
Danay Carrillo-Nieves, Héctor A Ruiz, Cristóbal N Aguilar, Anna Ilyina, Roberto Parra-Saldivar, J Antonio Torres, José L Martínez Hernández
Three alternatives for bioethanol production from pretreated mango stem bark after maceration (MSBAM) were evaluated as a biorefinery component for the mango agroindustry. These included separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and pre-saccharification followed by simultaneous saccharification and fermentation (PSSF). The effects on ethanol concentration, yield and productivity of pretreated MSBAM solids loading, Tween 20 addition, and temperature were used for process comparisons...
September 2017: Bioresource Technology
José Carlos Martínez-Patiño, Encarnación Ruiz, Inmaculada Romero, Cristóbal Cara, Juan Carlos López-Linares, Eulogio Castro
Olive tree biomass (OTB) can be used for producing second generation bioethanol. In this work, extracted OTB was subjected to fractionation using a sequential acid/alkaline oxidative pretreatment. In the first acid stage, the effects of sulfuric acid concentration and reaction times at 130°C were investigated. Up to 71% solubilization of hemicellulosic sugars was achieved under optimized conditions (2.4% H2SO4, 84min). In the second stage, the influence of hydrogen peroxide concentration and process time were evaluated at 80°C...
September 2017: Bioresource Technology
Halimatun Saadiah Hafid, Abdul Rahman Nor 'Aini, Mohd Noriznan Mokhtar, Ahmad Tarmezee Talib, Azhari Samsu Baharuddin, Md Shah Umi Kalsom
In Malaysia, the amount of food waste produced is estimated at approximately 70% of total municipal solid waste generated and characterised by high amount of carbohydrate polymers such as starch, cellulose, and sugars. Considering the beneficial organic fraction contained, its utilization as an alternative substrate specifically for bioethanol production has receiving more attention. However, the sustainable production of bioethanol from food waste is linked to the efficient pretreatment needed for higher production of fermentable sugar prior to fermentation...
May 17, 2017: Waste Management
Borja Lagoa-Costa, Haris Nalakath Abubackar, María Fernández-Romasanta, Christian Kennes, María C Veiga
Syngas bioconversion is a promising method for bioethanol production, but some VFA remains at the end of fermentation. A two-stage process was set-up, including syngas fermentation as first stage under strict anaerobic conditions using C. autoethanogenum as inoculum, with syngas (CO/CO2/H2/N2, 30/10/20/40) as gaseous substrate. The second stage consisted in various fed-batch assays using a highly enriched PHA accumulating biomass as inoculum, where the potential for biopolymer production from the remaining acetic acid at the end of the syngas fermentation was evaluated...
September 2017: Bioresource Technology
Chun-Han Ko, Fan-Chun Yu, Fang-Chih Chang, Bing-Yuan Yang, Wen-Hua Chen, Wen-Song Hwang, Ta-Chih Tu
Using plants to absorb and accumulate heavy metals from polluted soil, followed by the recycling of explants containing heavy metals, can help achieve the goal of reverting contaminated soil to low heavy-metal content soil. However, the re-use of recovered explants can also be problematic. Meanwhile, bioethanol has become a popular energy source. In this study, napier grass was used for the remediation of soil contaminated with heavy metals (artificially contaminated soil). The influence of bioethanol production from napier grass after phytoremediation was also investigated...
May 11, 2017: Journal of Environmental Management
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