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https://www.readbyqxmd.com/read/28107376/deletion-of-ph-regulator-pac-3-affects-cellulase-and-xylanase-activity-during-sugarcane-bagasse-degradation-by-neurospora-crassa
#1
Amanda Cristina Campos Antoniêto, Wellington Ramos Pedersoli, Lílian Dos Santos Castro, Rodrigo da Silva Santos, Aline Helena da Silva Cruz, Karoline Maria Vieira Nogueira, Rafael Silva-Rocha, Antonio Rossi, Roberto Nascimento Silva
Microorganisms play a vital role in bioethanol production whose usage as fuel energy is increasing worldwide. The filamentous fungus Neurospora crassa synthesize and secrete the major enzymes involved in plant cell wall deconstruction. The production of cellulases and hemicellulases is known to be affected by the environmental pH; however, the regulatory mechanisms of this process are still poorly understood. In this study, we investigated the role of the pH regulator PAC-3 in N. crassa during their growth on sugarcane bagasse at different pH conditions...
2017: PloS One
https://www.readbyqxmd.com/read/28101807/direct-bioethanol-production-from-wheat-straw-using-xylose-glucose-co-fermentation-by-co-culture-of-two-recombinant-yeasts
#2
Yuanyuan Zhang, Caiyun Wang, Lulu Wang, Ruoxin Yang, Peilei Hou, Junhong Liu
To achieve a cost-effective bioconversion of lignocellulosic materials, a novel xylose/glucose co-fermentation process by co-culture of cellulose-utilizing recombinant Saccharomyces cerevisiae (S. cerevisiae) and xylan-utilizing recombinant Pichia pastoris (P. pastoris) was developed, in which ethanol was produced directly from wheat straw without additional hydrolytic enzymes. Recombinant S. cerevisiae coexpressing three types of cellulase and recombinant P. pastoris coexpressing two types of xylanase were constructed, respectively...
January 18, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28099889/evaluation-of-organosolv-pretreatment-on-the-structural-characteristics-of-lignin-polymers-and-follow-up-enzymatic-hydrolysis-of-the-substrates-from-eucalyptus-wood
#3
Bing Wang, Xiao-Jun Shen, Jia-Long Wen, Lin Xiao, Run-Cang Sun
In the present study, Eucalyptus was subjected to organosolv pretreatment processes with aqueous 2-propanol at 200 to 220°C to obtain lignin with benign characters for its valorization and digestible substrates for bioethanol production. Results showed that different delignification ratios (64.00%-81.26%) and molecular weights (Mw=610-2680g/mol) of lignin fractions were dissociated from various pretreatment conditions, and the glucose yields of all the pretreated substrates significantly increased to 54.65-88...
January 15, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28097168/purification-and-biochemical-properties-of-a-thermostable-haloalkaline-cellulase-from-bacillus-licheniformis-amf-07-and-its-application-for-hydrolysis-of-different-cellulosic-substrates-to-bioethanol-production
#4
Fatemeh Azadian, Arastoo Badoei-Dalfard, Abdolhamid Namaki-Shoushtari, Mehdi Hassanshahian
A thermophilic strain AMF-07, hydrolyzing carboxymethylcellulose (CMC) was isolated from Kerman hot spring and was identified as Bacillus licheniformis based on 16S rRNA sequence homology. The carboxymethylcellulase (CMCase) enzyme produced by the B. licheniformis was purified by (NH4)2SO4 precipitation, ion exchange and gel filtration chromatography. The purified enzyme gave a single band on SDS- PAGE with a molecular weight of 37 kDa. The CMCase enzyme was highly active and stable over broad ranges of temperature (40-80ºC), pH (6...
September 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28089136/recent-insights-into-biohydrogen-production-by-microalgae-from-biophotolysis-to-dark-fermentation
#5
REVIEW
Dillirani Nagarajan, Duu-Jong Lee, Akihiko Kondo, Jo-Shu Chang
One of the best options to alleviate the problems associated with global warming and climate change is to reduce burning of fossil fuels and search for new alternative energy resources. In case of biodiesel and bioethanol production, the choice of feedstock and the process design influences the GHG emissions and appropriate methods need to be adapted. Hydrogen is a zero-carbon and energy dense alternative energy carrier with clean burning properties and biohydrogen production by microalgae can reduce production associated GHG emissions to a great extent...
December 30, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/28088640/bioethanol-production-from-cellulosic-hydrolysates-by-engineered-industrial-saccharomyces-cerevisiae
#6
Ye-Gi Lee, Yong-Su Jin, Young-Lok Cha, Jin-Ho Seo
Even though industrial yeast strains exhibit numerous advantageous traits for the production of bioethanol, their genetic manipulation has been limited. This study demonstrates that an industrial polyploidy Saccharomyces cerevisiae JHS200 can be engineered through Cas9 (CRISPR associated protein 9)-based genome editing. Specifically, we generated auxotrophic mutants and introduced a xylose metabolic pathway into the auxotrophic mutants. As expected, the engineered strain (JX123) enhanced ethanol production from cellulosic hydrolysates as compared to other engineered haploid strains...
December 18, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/28081526/phosphomolybdic-acid-and-ferric-iron-as-efficient-electron-mediators-for-coupling-biomass-pretreatment-to-produce-bioethanol-and-electricity-generation-from-wheat-straw
#7
Yi Ding, Bo Du, Xuebing Zhao, J Y Zhu, Dehua Liu
Phosphomolybdic acid (PMo12) was used as an electron mediator and proton carrier to mediate biomass pretreatment for ethanol production and electricity generation from wheat straw. In the pretreatment, lignin was oxidized anaerobically by PMo12 with solubilization of a fraction of hemicelluloses, and the PMo12 was simultaneously reduced. In an external liquid flow cell, the reduced PMo12 was re-oxidized with generation of electricity. The effects of several factors on pretreatment were investigated for optimizing the conditions...
January 3, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28081524/effects-of-acidified-aqueous-glycerol-and-glycerol-carbonate-pretreatment-of-rice-husk-on-the-enzymatic-digestibility-structural-characteristics-and-bioethanol-production
#8
Majid Ebrahimi, Oliver B Villaflores, Emma E Ordono, Alvin R Caparanga
Rice husk as an abundant biomass was used in this study, and it contained 30.1% glucan and 13.5% xylan, 22.4% lignin. The pretreated rice husk with glycerol carbonate and acidified aqueous glycerol (10% water) at 90°C and 130°C for 60min had the maximum yield of glucan digestibility which was 78.2% and 69.7% respectively, using cellulase for 72h. The simultaneous saccharification and fermentation was conducted anaerobically at 37°C with Saccharomyces cerevisiae, 5% w/v glucan and 10FPU/g glucan of cellulase...
January 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28074753/conversion-of-wastes-to-bioelectricity-bioethanol-and-fertilizer
#9
Abdul Majeed
This research article presents production of bioelectricity, bioethanol and fertilizer from different industrial wastewaters supplemented with waste fruit and vegetables. Bioelectricity was generated from wastewater through the development of different microbial fuel cells (MFCs). It was observed that the voltage was increased in series combination whereas current was increased in parallel combinations. The series combination of four units of single chamber and eight units of double chamber MFCs produced the power output of 5...
January 4, 2017: Water Environment Research: a Research Publication of the Water Environment Federation
https://www.readbyqxmd.com/read/28063510/converting-citrus-wastes-into-value-added-products-economic-and-environmently-friendly-approaches
#10
REVIEW
Kavita Sharma, Neelima Mahato, Moo Hwan Cho, Yong Rok Lee
Citrus fruits, including oranges, grapefruits, lemons, limes, tangerines, and mandarins, are among the most widely cultivated fruits around the globe. Its production is increasing every year due to rising consumer demand. Citrus-processing industries generate huge amounts of wastes every year, and citrus peel waste alone accounts for almost 50% of the wet fruit mass. Citrus waste is of immense economic value as it contains an abundance of various flavonoids, carotenoids, dietary fiber, sugars, polyphenols, essential oils, and ascorbic acid, as well as considerable amounts of some trace elements...
February 2017: Nutrition
https://www.readbyqxmd.com/read/28063146/pretreatment-optimization-of-the-biomass-of-microcystis-aeruginosa-for-efficient-bioethanol-production
#11
Muhammad Imran Khan, Moon Geon Lee, Jin Hyuk Shin, Jong Deog Kim
Microalgae are considered to be the future promising sources of biofuels and bio products. The algal carbohydrates can be fermented to bioethanol after pretreatment process. Efficient pretreatment of the biomass is one of the major requirements for commercialization of the algal based biofuels. In present study the microalga, M. aeruginsa was used for pretreatment optimization and bioethanol production. Treatment of algal biomass with CaO before acid and/or enzymatic hydrolysis enhanced the degradation of algal cells...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28062914/determination-of-kinetics-and-heat-of-hydrolysis-for-non-homogenous-substrate-by-isothermal-calorimetry
#12
D Tafoukt, A Soric, J-C Sigoillot, J-H Ferrasse
The competitiveness of the second-generation bioethanol by biotechnological process requires an effective and quantitative control of biochemical reactions. In this study, the potential of isothermal calorimetry technique to measure heat and kinetics of a non-homogeneous substrate enzymatic hydrolysis is intended. Using this technique, optimum temperature of the enzymes used for lignocellulosic molecules hydrolysis was determined. Thus, the amount of substrate-to-enzyme ratio was highlighted as an important parameter of the hydrolysis yield...
January 6, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28057426/bioethanol-strains-of-saccharomyces-cerevisiae-characterised-by-microsatellite-and-stress-resistance
#13
Vanda Renata Reis, Ana Teresa Burlamaqui Faraco Antonangelo, Ana Paula Guarnieri Bassi, Débora Colombi, Sandra Regina Ceccato-Antonini
Strains of Saccharomyces cerevisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cerevisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S...
December 22, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28053664/the-coptotermes-gestroi-aldo-keto-reductase-a-multipurpose-enzyme-for-biorefinery-applications
#14
Robson Tramontina, João Paulo L Franco Cairo, Marcelo V Liberato, Fernanda Mandelli, Amanda Sousa, Samantha Santos, Sarita Cândida Rabelo, Bruna Campos, Jaciane Ienczak, Roberto Ruller, André R L Damásio, Fabio Marcio Squina
BACKGROUND: In nature, termites can be considered as a model biological system for biofuel research based on their remarkable efficiency for lignocellulosic biomass conversion. Redox enzymes are of interest in second-generation ethanol production because they promote synergic enzymatic activity with classical hydrolases for lignocellulose saccharification and inactivate fermentation inhibitory compounds produced after lignocellulose pretreatment steps. RESULTS: In the present study, the biochemical and structural characteristics of the Coptotermes gestroi aldo-keto reductase (CgAKR-1) were comprehensively investigated...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28025673/wet-fractionation-of-the-succulent-halophyte-salicornia-sinus-persica-with-the-aim-of-low-input-water-saving-biorefining-into-bioethanol
#15
Ayah Alassali, Iwona Cybulska, Alejandro Ríos Galvan, Mette Hedegaard Thomsen
In this study Salicornia sinus-persica, a succulent halophyte was assessed for its potential to be used as a feedstock for bioethanol production. For such succulent, salty, green biomasses, direct fractionation and fermentation allow for water preservation in the process. Fresh biomass of S. sinus-persica was collected and split into two fractions by wet fractionation; liquid (juice) and solid (pulp). Sugar contents were found to be 1.0-1.5% for the juice fraction and 50% (w/w) for the fresh pulp. Direct fermentation of the juice using Saccharomyces cerevisiae showed no salt inhibition of the yeast and ethanol yields of ~70% were achieved...
December 26, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28018390/emerging-technologies-for-the-production-of-renewable-liquid-transport-fuels-from-biomass-sources-enriched-in-plant-cell-walls
#16
REVIEW
Hwei-Ting Tan, Kendall R Corbin, Geoffrey B Fincher
Plant cell walls are composed predominantly of cellulose, a range of non-cellulosic polysaccharides and lignin. The walls account for a large proportion not only of crop residues such as wheat straw and sugarcane bagasse, but also of residues of the timber industry and specialist grasses and other plants being grown specifically for biofuel production. The polysaccharide components of plant cell walls have long been recognized as an extraordinarily large source of fermentable sugars that might be used for the production of bioethanol and other renewable liquid transport fuels...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28010766/heterologous-expression-in-pichia-pastoris-and-characterization-of-a-%C3%AE-glucosidase-from-the-xylophagous-cockroach-panesthia-angustipennis-spadica-displaying-high-specific-activity-for-cellobiose
#17
Yihai Li, Gaku Arakawa, Gaku Tokuda, Hirofumi Watanabe, Manabu Arioka
A β-glucosidase (BG), PaBG1b, from the xylophagous cockroach Panesthia angustipennis spadica was heterologously expressed in the methylotrophic yeast Pichia pastoris, purified, and biochemically characterized. Post-translational modification and N-terminal sequencing analysis demonstrated that the expression product was comprised of two polypeptides with different N-terminal sequences, presumably due to the presence of lysine-arginine (KR) sequence in the putative mature region. Substrate specificity analysis showed that PaBG1b hydrolyzed a broad range of substrates including cellohexaose, with the preference for aryl β-d-fucosyl linkage and laminaribiose...
February 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28005227/in-planta-production-and-characterization-of-a-hyperthermostable-gh10-xylanase-in-transgenic-sugarcane
#18
Jae Yoon Kim, Guang Nong, John D Rice, Maria Gallo, James F Preston, Fredy Altpeter
Sugarcane (Saccharum sp. hybrids) is one of the most efficient and sustainable feedstocks for commercial production of fuel ethanol. Recent efforts focus on the integration of first and second generation bioethanol conversion technologies for sugarcane to increase biofuel yields. This integrated process will utilize both the cell wall bound sugars of the abundant lignocellulosic sugarcane residues in addition to the sucrose from stem internodes. Enzymatic hydrolysis of lignocellulosic biomass into its component sugars requires significant amounts of cell wall degrading enzymes...
December 22, 2016: Plant Molecular Biology
https://www.readbyqxmd.com/read/28004152/comparative-transcriptomes-reveal-novel-evolutionary-strategies-adopted-by-saccharomyces-cerevisiae-with-improved-xylose-utilization-capability
#19
Wei-Yi Zeng, Yue-Qin Tang, Min Gou, Zhao-Yong Sun, Zi-Yuan Xia, Kenji Kida
Production of ethanol from xylose by recombinant Saccharomyces cerevisiae is suboptimal with slow fermentation rate, compared with that from glucose. In this study, a strain-expressing Scheffersomyces stipitis xylose reductase-xylitol dehydrogenase (XR-XDH) pathway was subjected to adaptive evolution on xylose; this approach generated populations with the significantly improved cell growth and ethanol production rate. Mutants were isolated, and the best one was used for sporulation to generate eight stable mutant strains with improved xylose fermentation ability...
December 21, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27997079/optimization-of-enzymatic-hydrolysis-and-fermentation-conditions-for-improved-bioethanol-production-from-potato-peel-residues
#20
Imen Ben Taher, Patrick Fickers, Sofien Chniti, Mnasser Hassouna
The aim of this work was the optimization of the enzyme hydrolysis of potato peel residues (PPR) for bioethanol production. The process included a pretreatment step followed by an enzyme hydrolysis using crude enzyme system composed of cellulase, amylase and hemicellulase, produced by a mixed culture of Aspergillus niger and Trichoderma reesei. Hydrothermal, alkali and acid pretreatments were considered with regards to the enhancement of enzyme hydrolysis of potato peel residues. The obtained results showed that hydrothermal pretreatment lead to a higher enzyme hydrolysis yield compared to both acid and alkali pretreatments...
December 20, 2016: Biotechnology Progress
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