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Timothy Howdeshell, Takuji Tanaka
A bioethanol byproduct (dried distiller's grains with solubles; DDGS) contains a high cellulose and starch. We hypothesized combinations of solid-state fermentation (SSF) and digestion by black soldier fly larvae (BSFL) (Hermetia illucens) could increase the recovery of glucose from this byproduct through concentrating and loosening cellulose matrix by their activities. DDGS was individually fermented with Aspergillus niger, Aspergillus fumigatus, Trichoderma koningii, Phanerochaete chrysosporium, or Lactobacillus plantarum...
May 18, 2018: Canadian Journal of Microbiology
Shaolong Sun, Lidan Zhang, Fang Liu, Xiaolin Fan, Run-Cang Sun
Background: To increase the production of bioethanol, a two-step process based on hydrothermal and dilute alkaline treatment was applied to reduce the natural resistance of biomass. However, the process required a large amount of water and a long operation time due to the solid/liquid separation before the alkaline treatment, which led to decrease the pure economic profit for production of bioethanol. Therefore, four one-step processes based on order of hydrothermal and alkaline treatment have been developed to enhance concentration of glucose of wheat straw by enzymatic saccharification...
2018: Biotechnology for Biofuels
Ioannis Papapetridis, Maarten D Verhoeven, Sanne J Wiersma, Maaike Goudriaan, Antonius J A van Maris, Jack T Pronk
Simultaneous fermentation of glucose and xylose can contribute to improved productivity and robustness of yeast-based processes for bioethanol production from lignocellulosic hydrolysates. This study explores a novel laboratory evolution strategy for identifying mutations that contribute to simultaneous utilization of these sugars in batch cultures of Saccharomyces cerevisiae. To force simultaneous utilization of xylose and glucose, the genes encoding glucose-6-phosphate isomerase (PGI1) and ribulose-5-phosphate epimerase (RPE1) were deleted in a xylose-isomerase-based xylose-fermenting strain with a modified oxidative pentose-phosphate pathway...
May 15, 2018: FEMS Yeast Research
N Gurdo, G F Novelli Poisson, Á B Juárez, M C Ríos de Molina, M A Galvagno
AIMS: To investigate multiple tolerance of Saccharomyces cerevisiae obtained through a laboratory strategy of adaptive evolution in acetic acid, its relation with enzymatic ROS detoxification and bioethanol 2G production. METHODS AND RESULTS: After adaptive evolution in acetic acid, a clone (Y8A) was selected for its tolerance to high acetic acid concentrations (13 g l-1 ) in batch cultures. Y8A was resistant to multiple stresses: osmotic, thermic, oxidative, saline, ethanol, organic acid, phenolic compounds and slow freeze-thawing cycles...
May 16, 2018: Journal of Applied Microbiology
Negar Jafari, Razieh Shafiee Alavijeh, Ali Abdolahnejad, Hossein Farrokhzadeh, Mohammad Mehdi Amin, Afshin Ebrahimi
This article investigates the innovative attached cultivation of Chlorella vulgaris (C. vulgaris) using different materials as an alternative to high capital techniques of harvesting such as centrifugation, flocculation, and filtration. A simple attached algal cultivation system was proposed that was equipped by 10 submerged supporting materials which can harvest algal cells, efficiently. The effect of operational parameters such as light intensity, the rate of aeration, and auto-harvesting time was investigated...
May 10, 2018: Environmental Science and Pollution Research International
Amal A Elgharbawy, Md Zahangir Alam, Muhammad Moniruzzaman, Nassereldeen Ahmad Kabbashi, Parveen Jamal
The pretreatment of empty fruit bunch (EFB) was conducted using an integrated system of IL and cellulases (IL-E), with simultaneous fermentation in one vessel. The cellulase mixture ( PKC-Cel ) was derived from Trichoderma reesei by solid-state fermentation. Choline acetate [Cho]OAc was utilized for the pretreatment due to its biocompatibility and biodegradability. The treated EFB and its hydrolysate were characterized by the Fourier transform infrared spectroscopy, scanning electron microscopy, and chemical analysis...
May 2018: 3 Biotech
Alan Rempel, Tainara Machado, Helen Treichel, Eliane Colla, Ana Cláudia Margarites, Luciane Maria Colla
We aimed to use physical methods of microalgal biomass rupture to study saccharification strategies using free and immobilized amylolytic enzymes. The biomass of Spirulina platensis, which consists of 50-60% carbohydrates, was exposed to physical cell rupture treatments, with better results obtained using freeze/thaw cycles following by gelatinization. In saccharification tests, it was possible to hydrolyze Spirulina biomass with hydrolysis efficiencies above 99% and 83%, respectively, using 1% (v/v) of free enzymes or 1% (m/v) of amylolytic enzymes immobilized together...
April 30, 2018: Bioresource Technology
Natthaya Mangkorn, Pattanop Kanokratana, Niran Roongsawang, Navadol Laosiripojana, Verawat Champreda
Alcohol oxidase (AOX) functions in oxidation of primary alcohols into the corresponding aldehydes with potential on catalyzing synthesis reactions in chemical industry. In this study, AOX from a thermotolerant methylotrophic yeast, Ogataea thermomethanolica (OthAOX) was purified to high homogeneity using a single step chromatographic separation on a DEAE-Sepharose column. The purified OthAOX had a specific activity of 15.34 U/mg with 77.5% recovery yield. The enzyme worked optimally at 50 °C in an alkaline range (pH 9...
May 5, 2018: Protein Expression and Purification
Daniela L Aguilar, Rosa M Rodríguez-Jasso, Elisa Zanuso, Diana Jasso de Rodríguez, Lorena Amaya-Delgado, Arturo Sanchez, Héctor A Ruiz
The production of tequila in Mexico generates a large amount of agave bagasse per year. However, this biomass can be considered as a potential source for biofuel production. In this study, it is described how the hydrothermal pretreatment was scaled in a bench scale, considering the severity index as a strategy. The best condition was at 180 °C in isothermal regime for 20 min with 65.87% of cellulose content and high concentration of xylooligosaccharides (15.31 g/L). This condition was scaled up (using severity factor: [logR0 ] = 4...
April 30, 2018: Bioresource Technology
Jérémie Damay, Iulian-Zoltan Boboescu, Xavier Duret, Olivier Lalonde, Jean-Michel Lavoie
Sweet sorghum was subjected to an impregnation step, which recovered most of the 1st generation sugars, prior to a steam-treatment extraction of the 2nd generation sugars, at three different severity factors (SF). A medium severity (3.56 SF) treatment proved to be an optimal compromise between the amount of sugars extracted and the fermentation inhibitors generated following the subsequent depolymerization approaches applied on the broth. Next, a series of detoxification approaches (ozonation, overliming and a combination of both) were investigated following a concentration and depolymerization step...
April 13, 2018: Bioresource Technology
Nisha Singh, Munish Puri, Deepak K Tuli, Ravi P Gupta, Colin J Barrow, Anshu S Mathur
To overcome the challenges associated with combined bioprocessing of lignocellulosic biomass to biofuel, finding good organisms is essential. An ethanol producing bacteria DBT-IOC-DC21 was isolated from a compost site via preliminary enrichment culture on a pure hemicellulosic substrate and identified as a Clostridium strain by 16S rRNA analysis. This strain presented broad substrate spectrum with ethanol, acetate, lactate, and hydrogen as the primary metabolic end products. The optimum conditions for ethanol production were found to be an initial pH of 7...
May 2, 2018: Anaerobe
Angela Boyce, Gary Walsh
A locally isolated strain of Aspergillus niger van Tieghem was found to produce thermostable β-xylosidase activity. The enzyme was purified by cation and anion exchange and hydrophobic interaction chromatography. Maximum activity was observed at 70-75 °C and pH 4.5. The enzyme was found to be thermostable retaining 91 and 87% of its original activity after incubation for 72 h at 60 and 65 °C, respectively, with 52% residual activity detected after 18 h at 70 °C. Available data indicates that the purified β-xylosidase is more thermostable over industrially relevant prolonged periods at high temperature than those reported from other A...
May 5, 2018: Applied Biochemistry and Biotechnology
Lei Qin, Xiong Zhao, Wen-Chao Li, Jia-Qing Zhu, Li Liu, Bing-Zhi Li, Ying-Jin Yuan
Background: Improving ethanol concentration and reducing enzyme dosage are main challenges in bioethanol refinery from lignocellulosic biomass. Ethylenediamine (EDA) pretreatment is a novel method to improve enzymatic digestibility of lignocellulose. In this study, simultaneous saccharification and co-fermentation (SSCF) process using EDA-pretreated corn stover was analyzed and optimized to verify the constraint factors on ethanol production. Results: Highest ethanol concentration was achieved with the following optimized SSCF conditions at 6% glucan loading: 12-h pre-hydrolysis, 34 °C, pH 5...
2018: Biotechnology for Biofuels
Yusuke Sasaki, Toshiyuki Takagi, Keisuke Motone, Toshiyuki Shibata, Kouichi Kuroda, Mitsuyoshi Ueda
A co-culture platform for bioethanol production from brown macroalgae was developed, consisting of two types of engineered Saccharomyces cerevisiae strains; alginate- and mannitol-assimilating yeast (AM1), and cellulase-displaying yeast (CDY). When the 5% (w/v) brown macroalgae Ecklonia kurome was used as the sole carbon source for this system, 2.1 g/L of ethanol was produced, along with simultaneous consumption of alginate, mannitol, and glucans.
April 30, 2018: Bioscience, Biotechnology, and Biochemistry
Késia S Lourenço, Noriko A Cassman, Agata S Pijl, Johannes A van Veen, Heitor Cantarella, Eiko E Kuramae
Organic vinasse, a residue produced during bioethanol production, increases nitrous oxide (N2 O) emissions when applied with inorganic nitrogen (N) fertilizer in soil. The present study investigated the role of the ammonia-oxidizing bacteria (AOB) community on the N2 O emissions in soils amended with organic vinasse (CV: concentrated and V: non-concentrated) plus inorganic N fertilizer. Soil samples and N2 O emissions were evaluated at 11, 19, and 45 days after fertilizer application, and the bacterial and archaea gene ( amo A) encoding the ammonia monooxygenase enzyme, bacterial denitrifier ( nir K, nir S, and nos Z) genes and total bacteria were quantified by real time PCR...
2018: Frontiers in Microbiology
Yeshona Sewsynker-Sukai, E B Gueguim Kana
This study investigates the simultaneous saccharification and fermentation (SSF) process for bioethanol production from corn cobs with prehydrolysis (PSSF) and without prehydrolysis (OSSF). Two response surface models were developed with high coefficients of determination (>0.90). Process optimization gave high bioethanol concentrations and bioethanol conversions for the PSSF (36.92 ± 1.34 g/L and 62.36 ± 2.27%) and OSSF (35.04 ± 0.170 g/L and 58.13 ± 0.283%) models respectively. Additionally, the logistic and modified Gompertz models were used to study the kinetics of microbial cell growth and ethanol formation under microaerophilic and anaerobic conditions...
April 17, 2018: Bioresource Technology
Shan Wei, Yanan Liu, Meiling Wu, Tiantai Ma, Xiangzheng Bai, Jin Hou, Yu Shen, Xiaoming Bao
Background: The recombinant Saccharomyces cerevisiae strains that acquired the ability to utilize xylose through metabolic and evolutionary engineering exhibit good performance when xylose is the sole carbon source in the medium (designated the X stage in the present work). However, the xylose consumption rate of strains is generally low after glucose depletion during glucose-xylose co-fermentation, despite the presence of xylose in the medium (designated the GX stage in the present work)...
2018: Biotechnology for Biofuels
Daniel Gomes, Miguel Gama, Lucília Domingues
Background: In spite of the continuous efforts and investments in the last decades, lignocellulosic ethanol is still not economically competitive with fossil fuels. Optimization is still required in different parts of the process. Namely, the cost effective usage of enzymes has been pursued by different strategies, one of them being recycling. Results: Cellulase recycling was analyzed on recycled paper sludge (RPS) conversion into bioethanol under intensified conditions...
2018: Biotechnology for Biofuels
Chaochen Tang, Songbo Li, Meng Li, Guang H Xie
Field experiments were conducted in marginal lands, i.e., sub-humid climate and saline-land (SHS) and semi-arid climate and wasteland (SAW), to evaluate ethanol potential based on the biomass yield and chemical composition of biomass type (var. GN-2, GN-4, and GN-10) and sweet type (var. GT-3 and GT-7) hybrids of energy sorghum [ Sorghum bicolor (L.) Moench] in comparison with sub-humid climate and cropland (SHC) in northern China. Results showed that environment significantly ( p < 0.05) influenced plant growth, biomass yield and components, and subsequently the ethanol potential of energy sorghum...
2018: Frontiers in Plant Science
Yueying Chu, Xiuzhi Gao, Xin Zhang, Guangtong Xu, Guangchao Li, Anmin Zheng
In this work, a density functional theory (DFT) study was carried out to address the fundamental description of the effective phosphorous species that could improve the framework stability and reduce the coke deposition formation on the P-ZSM-5 zeolite. On the basis of the dealumination barriers of ZSM-5 with all the possible phosphorous species bound on the zeolite framework, PO4H4 was ascertained to be the effective phosphorous species that could improve the ZSM-5 zeolite hydrothermal stability and reduce its acid strength...
April 23, 2018: Physical Chemistry Chemical Physics: PCCP
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