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Bioethanol

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https://www.readbyqxmd.com/read/28811016/activated-biochar-preparation-characterization-and-electroanalytical-application-in-an-alternative-strategy-of-nickel-determination
#1
Cristiane Kalinke, Paulo Roberto Oliveira, Geovane Arruda Oliveira, Antonio Sálvio Mangrich, Luiz H Marcolino-Junior, Márcio F Bergamini
This work reports for the first time the use of chemically activated biochar as electrode modifier for nickel determination. The biochar activation was performed by refluxing with HNO3, which promoted a higher nickel preconcentration compared to unmodified and modified biochar precursor electrodes. Morphological and structural characterization revealed the increase of surface acid groups, surface area and porosity of biochar after activation. Nickel determination was investigated adopting an alternative voltammetric methodology based on monitoring the Ni(II)/Ni(III) redox couple...
August 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28804804/life-cycle-environmental-impacts-of-bioethanol-production-from-sugarcane-molasses-in-iran
#2
Saeid Shahvarooghi Farahani, Mohammad Amin Asoodar
In recent years, bioethanol from sugarcane molasses has been produced on an industrial scale in Iran. The aim of this study was to evaluate molasses-based bioethanol production from an environmental point of view. Data were collected from Debel Khazai agro-industry situated in southern region of Iran by using face-to-face interviews and annual statistics of 2010 to 2016 (6-year life cycle of sugarcane cultivation). Ten impact categories including abiotic depletion (AD), acidification (AC), eutrophication (EP), global warming potential (GWP), ozone layer depletion (OLD), human toxicity (HT), freshwater aquatic ecotoxicity (FE), marine aquatic ecotoxicity (ME), terrestrial ecotoxicity (TE), and photochemical oxidation (PO) were selected based on CML methodology...
August 13, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28799253/production-of-bioethanol-in-sugarcane-bagasse-hemicellulosic-hydrolysate-by-scheffersomyces-parashehatae-scheffersomyces-illinoinensis-and-spathaspora-arborariae-isolated-from-brazilian-ecosystems
#3
R M Cadete, M A Melo-Cheab, K J Dussán, R C L B Rodrigues, S S da Silva, F C O Gomes, C A Rosa
AIMS: This study aimed to evaluate new d-xylose-fermenting yeasts from Brazilian ecosystems for the production of second-generation ethanol. METHODS AND RESULTS: d-xylose-fermenting yeasts isolated from rotting wood and wood-boring insects were identified as the species Scheffersomyces parashehatae, Sc. illinoinensis, Spathaspora arborariae, and Wickerhamomyces rabaulensis. Among the yeasts tested, those of Scheffersomyces parashehatae exhibited the highest ethanol production when cultivated on complex medium (Yp/s(et) = 0...
August 11, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28780270/development-of-natural-cellulase-inhibitor-mediated-intensified-biological-pretreatment-technology-using-pleurotus-florida-for-maximum-recovery-of-cellulose-from-paddy-straw-under-solid-state-condition
#4
Manickam Naresh Kumar, Rajarathinam Ravikumar, Senniyappan Thenmozhi, Muthuvelu Kirupa Sankar
Inhibitor mediated intensified bio-pretreatment (IMBP) technology using natural cellulase inhibitor (NCI) for maximum cellulose recovery from paddy straw was studied. Pretreatment was carried out under solid state condition. Supplementation of 8% NCI in pretreatment medium improves cellulose recovery and delignification by 1.2 and 1.5-fold respectively, compared to conventional bio-pretreatment due to inhibition of 61% of cellulase activity in IMBP. Further increase in NCI concentration showed negative effect on Pleurotus florida growth and suppress the laccase productivity by 1...
July 22, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28777990/additives-enhancing-enzymatic-hydrolysis-of-lignocellulosic-biomass
#5
Javier Rocha-Martín, Claudio Martinez-Bernal, Yolanda Pérez-Cobas, Francisco Manuel Reyes-Sosa, Bruno Díez García
Linked to the development of cellulolytic enzyme cocktails from Myceliophthora thermophila, we studied the effect of different additives on the enzymatic hydrolysis yield. The hydrolysis of pretreated corn stover (PCS), sugar cane straw (PSCS) and microcrystalline cellulose (Avicel) was performed under industrial conditions using high solid loadings, limited mixing, and low enzyme dosages. The addition of polyethylene glycol (PEG4000) allowed to increase the glucose yields by 10%, 7.5%, and 32%, respectively in the three materials...
June 26, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28770369/bioethanol-production-from-raw-sugar-beet-cossettes-in-horizontal-rotating-tubular-bioreactor
#6
Mladen Pavlečić, Tonči Rezić, Mirela Ivančić Šantek, Predrag Horvat, Božidar Šantek
Fossil fuels are still major energy sources, but the search for renewable energy sources has been encouraged. Bioethanol has been recognized as an alternative to fossil fuels and nowadays it represents more than 90% of the global biofuel production. Bioethanol production from raw sugar beet cossettes as a semi-solid substrate was studied. The study was carried out in the horizontal rotating tubular bioreactor (HRTB) with Saccharomyces cerevisiae as a microbial production strain. The impact of different combinations of HRTB operational parameters such as, rotation speed (5-15 min(-1)), rotation type [constant or interval (3-15 min h(-1))] and working volume (ratio V W/V T = 0...
August 2, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28768815/synthesis-and-self-assembly-of-cellulose-microfibrils-from-reconstituted-cellulose-synthase
#7
Sung Hyun Cho, Pallinti Purushotham, Chao Fang, Cassandra Maranas, Sara M Díaz-Moreno, Vincent Bulone, Jochen Zimmer, Manish Kumar, B Tracy Nixon
Cellulose, the major component of plant cell walls, can be converted to bioethanol and is thus highly studied. In plants, cellulose is produced by cellulose synthase, a processive family-2 glycosyltransferase. In plant cell walls, individual β-1,4-glucan chains polymerized by CesA are assembled into microfibrils that are frequently bundled into macrofibrils. An in vitro system in which cellulose is synthesized and assembled into fibrils would facilitate detailed study of this process. Here, we report the heterologous expression and partial purification of His-tagged CesA5 from Physcomitrella patens...
August 2, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28758291/xylanase-production-from-marine-derived-trichoderma-pleuroticola-08%C3%A3-k001-strain-isolated-from-mediterranean-coastal-sediments
#8
Melih N Korkmaz, Sennur C Ozdemir, Ataç Uzel
Xylanases constitutes one the most important enzymes with diverse applications in different industries such as bioethanol production, animal feedstock production, production of xylo-oligosaccharides, baking industry, paper and pulp industry, xylitol production, fruit juice, and beer finishing, degumming, and agriculture. Currently, industrial xylanases are mainly produced by Aspergillus and Trichoderma members. Since the marine environments are less studied compared to terrestrial environments and harbors great microbial diversity we aimed to investigate the xylanase production of 88 marine-derived filamentous fungal strains...
July 31, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28756542/bioethanol-production-from-soybean-residue-via-separate-hydrolysis-and-fermentation
#9
Trung Hau Nguyen, Chae Hun Ra, In Yung Sunwoo, Pailin Sukwong, Gwi-Taek Jeong, Sung-Koo Kim
Bioethanol was produced using polysaccharide from soybean residue as biomass by separate hydrolysis and fermentation (SHF). This study focused on pretreatment, enzyme saccharification, and fermentation. Pretreatment to obtain monosaccharide was carried out with 20% (w/v) soybean residue slurry and 270 mmol/L H2SO4 at 121 °C for 60 min. More monosaccharide was obtained from enzymatic hydrolysis with a 16 U/mL mixture of commercial enzymes C-Tec 2 and Viscozyme L at 45 °C for 48 h. Ethanol fermentation with 20% (w/v) soybean residue hydrolysate was performed using wild-type and Saccharomyces cerevisiae KCCM 1129 adapted to high concentrations of galactose, using a flask and 5-L fermenter...
July 29, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28741083/construction-of-bioengineered-yeast-platform-for-direct-bioethanol-production-from-alginate-and-mannitol
#10
Toshiyuki Takagi, Yusuke Sasaki, Keisuke Motone, Toshiyuki Shibata, Reiji Tanaka, Hideo Miyake, Tetsushi Mori, Kouichi Kuroda, Mitsuyoshi Ueda
Brown macroalgae are a sustainable and promising source for bioethanol production because they are abundant in ocean ecosystems and contain negligible quantities of lignin. Brown macroalgae contain cellulose, hemicellulose, mannitol, laminarin, and alginate as major carbohydrates. Among these carbohydrates, brown macroalgae are characterized by high levels of alginate and mannitol. The direct bioconversion of alginate and mannitol into ethanol requires extensive bioengineering of assimilation processes in the standard industrial microbe Saccharomyces cerevisiae...
July 24, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28738551/biomass-saccharification-is-largely-enhanced-by-altering-wall-polymer-features-and-reducing-silicon-accumulation-in-rice-cultivars-harvested-from-nitrogen-fertilizer-supply
#11
Zahoor, Dan Sun, Ying Li, Jing Wang, Yuanyuan Tu, Yanting Wang, Zhen Hu, Shiguang Zhou, Lingqiang Wang, Guosheng Xie, Jianliang Huang, Aftab Alam, Liangcai Peng
In this study, two rice cultivars were collected from experimental fields with seven nitrogen fertilizer treatments. All biomass samples contained significantly increased cellulose contents and reduced silica levels, with variable amounts of hemicellulose and lignin from different nitrogen treatments. Under chemical (NaOH, CaO, H2SO4) and physical (hot water) pretreatments, biomass samples exhibited much enhanced hexoses yields from enzymatic hydrolysis, with high bioethanol production from yeast fermentation...
July 12, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28711801/effect-of-different-nitrogen-fertilizer-treatments-on-the-conversion-of-miscanthus%C3%A3-giganteus-to-ethanol
#12
Bogdan Dubis, Katarzyna Bułkowska, Małgorzata Lewandowska, Władysław Szempliński, Krzysztof Józef Jankowski, Jakub Idźkowski, Natalia Kordala, Karolina Szymańska
Miscanthus×giganteus is a perennial rhizomatous grass which is used as a biofuel crop. Due to its high yields, low production costs, resistance to low temperatures, low soil requirements and, above all, high cellulose content, miscanthus can be a useful resource for ethanol production. The aim of this study was to determine the effect of two fertilization regimes (sewage sludge/mineral NPK) during miscanthus cultivation on the chemical composition of biomass, the content of major lignocellulosic factions and the effectiveness of miscanthus conversion to bioethanol...
July 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28709070/ethanol-production-in-a-simultaneous-saccharification-and-fermentation-process-with-interconnected-reactors-employing-hydrodynamic-cavitation-pretreated-sugarcane-bagasse-as-raw-material
#13
Ruly Terán Hilares, João Vitor Ienny, Paulo Franco Marcelino, Muhammad Ajaz Ahmed, Felipe A F Antunes, Silvio Silvério da Silva, Júlio César Dos Santos
In this study, sugarcane bagasse (SCB) pretreated with alkali assisted hydrodynamic cavitation (HC) was investigated for simultaneous saccharification and fermentation (SSF) process for bioethanol production in interconnected column reactors using immobilized Scheffersomyces stipitis NRRL-Y7124. Initially, HC was employed for the evaluation of the reagent used in alkaline pretreatment. Alkalis (NaOH, KOH, Na2CO3, Ca(OH)2) and NaOH recycled black liquor (successive batches) were used and their pretreatment effectiveness was assessed considering the solid composition and its enzymatic digestibility...
July 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28704553/screening-and-characterizing-of-xylanolytic-and-xylose-fermenting-yeasts-isolated-from-the-wood-feeding-termite-reticulitermes-chinensis
#14
Sameh Samir Ali, Jian Wu, Rongrong Xie, Feng Zhou, Jianzhong Sun, Miao Huang
The effective fermentation of xylose remains an intractable challenge in bioethanol industry. The relevant xylanase enzyme is also in a high demand from industry for several biotechnological applications that inevitably in recent times led to many efforts for screening some novel microorganisms for better xylanase production and fermentation performance. Recently, it seems that wood-feeding termites can truly be considered as highly efficient natural bioreactors. The highly specialized gut systems of such insects are not yet fully realized, particularly, in xylose fermentation and xylanase production to advance industrial bioethanol technology as well as industrial applications of xylanases...
2017: PloS One
https://www.readbyqxmd.com/read/28702017/acetic-acid-causes-endoplasmic-reticulum-stress-and-induces-the-unfolded-protein-response-in-saccharomyces-cerevisiae
#15
Nozomi Kawazoe, Yukio Kimata, Shingo Izawa
Since acetic acid inhibits the growth and fermentation ability of Saccharomyces cerevisiae, it is one of the practical hindrances to the efficient production of bioethanol from a lignocellulosic biomass. Although extensive information is available on yeast response to acetic acid stress, the involvement of endoplasmic reticulum (ER) and unfolded protein response (UPR) has not been addressed. We herein demonstrated that acetic acid causes ER stress and induces the UPR. The accumulation of misfolded proteins in the ER and activation of Ire1p and Hac1p, an ER-stress sensor and ER stress-responsive transcription factor, respectively, were induced by a treatment with acetic acid stress (>0...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28690620/sugarcane-water-stress-tolerance-mechanisms-and-its-implications-on-developing-biotechnology-solutions
#16
REVIEW
Thais H S Ferreira, Max S Tsunada, Denis Bassi, Pedro Araújo, Lucia Mattiello, Giovanna V Guidelli, Germanna L Righetto, Vanessa R Gonçalves, Prakash Lakshmanan, Marcelo Menossi
Sugarcane is a unique crop with the ability to accumulate high levels of sugar and is a commercially viable source of biomass for bioelectricity and second-generation bioethanol. Water deficit is the single largest abiotic stress affecting sugarcane productivity and the development of water use efficient and drought tolerant cultivars is an imperative for all major sugarcane producing countries. This review summarizes the physiological and molecular studies on water deficit stress in sugarcane, with the aim to help formulate more effective research strategies for advancing our knowledge on genes and mechanisms underpinning plant response to water stress...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28690167/evaluation-of-lignins-from-side-streams-generated-in-an-olive-tree-pruning-based-biorefinery-bioethanol-production-and-alkaline-pulping
#17
José I Santos, Úrsula Fillat, Raquel Martín-Sampedro, María E Eugenio, María J Negro, Ignacio Ballesteros, Alejandro Rodríguez, David Ibarra
In modern lignocellulosic-based biorefineries, carbohydrates can be transformed into biofuels and pulp and paper, whereas lignin is burned to obtain energy. However, a part of lignin could be converted into value-added products including bio-based aromatic chemicals, as well as building blocks for materials. Then, a good knowledge of lignin is necessary to define its valorisation procedure. This study characterized different lignins from side-streams produced from olive tree pruning bioethanol production (lignins collected from steam explosion pretreatment with water or phosphoric acid as catalysts, followed by simultaneous saccharification and fermentation process) and alkaline pulping (lignins recovered from kraft and soda-AQ black liquors)...
July 6, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28688047/assessment-of-antioxidant-and-antimicrobial-properties-of-lignin-from-corn-stover-residue-pretreated-with-low-moisture-anhydrous-ammonia-and-enzymatic-hydrolysis-process
#18
Mingming Guo, Tony Jin, Nhuan P Nghiem, Xuetong Fan, Phoebe X Qi, Chan Ho Jang, Lingxiao Shao, Changqing Wu
Lignin accounts for 15-35% of dry biomass materials. Therefore, developing value-added co-products from lignin residues is increasingly important to improve the economic viability of biofuel production from biomass resources. The main objective of this work was to study the lignin extracts from corn stover residue obtained from a new and improved process for bioethanol production. Extraction conditions that favored high lignin yield were optimized, and antioxidant and antimicrobial activities of the resulting lignin were investigated...
July 7, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28683384/mild-chemical-pretreatments-are-sufficient-for-complete-saccharification-of-steam-exploded-residues-and-high-ethanol-production-in-desirable-wheat-accessions
#19
Zahoor, Yuanyuan Tu, Lingqiang Wang, Tao Xia, Dan Sun, Shiguang Zhou, Yanting Wang, Ying Li, Heping Zhang, Tong Zhang, Meysam Madadi, Liangcai Peng
In this study, a combined pretreatment was performed in four wheat accessions using steam explosion followed with different concentrations of H2SO4 or NaOH, leading to increased hexoses yields by 3-6 folds from enzymatic hydrolysis. Further co-supplied with 1% Tween-80, Talq90 and Talq16 accessions exhibited an almost complete enzymatic saccharification of steam-exploded (SE) residues after 0.5% H2SO4 or 1% NaOH pretreatment, with the highest bioethanol yields at 18.5%-19.4%, compared with previous reports about wheat bioethanol yields at 11%-17% obtained under relatively strong pretreatment conditions...
June 23, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28675841/comparison-of-microwave-and-conduction-convection-heating-autohydrolysis-pretreatment-for-bioethanol-production
#20
Alejandra Aguilar-Reynosa, Aloia Romaní, Rosa M Rodríguez-Jasso, Cristóbal N Aguilar, Gil Garrote, Héctor A Ruiz
This work describes the application of two forms of heating for autohydrolysis pretreatment on isothermal regimen: conduction-convection heating and microwave heating processing using corn stover as raw material for bioethanol production. Pretreatments were performed using different operational conditions: residence time (10-50 min) and temperature (160-200°C) for both pretreatments. Subsequently, the susceptibility of pretreated solids was studied using low enzyme loads, and high substrate loads. The highest conversion was 95...
June 20, 2017: Bioresource Technology
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